LOCUS       DAYYCM010000001       103076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000001 DAYYCM010000000
VERSION     DAYYCM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 103076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.001"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..3162)
                     /locus_tag="ACHAY1_000001"
     CDS             complement(<1..3162)
                     /locus_tag="ACHAY1_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592037.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANS"
     gene            complement(3285..4835)
                     /locus_tag="ACHAY1_000002"
     CDS             complement(3285..4835)
                     /locus_tag="ACHAY1_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8592038.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(4837..5202)
                     /locus_tag="ACHAY1_000003"
     CDS             complement(4837..5202)
                     /locus_tag="ACHAY1_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8592039.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(5250..6803)
                     /locus_tag="ACHAY1_000004"
     CDS             complement(5250..6803)
                     /locus_tag="ACHAY1_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8592040.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(6864..7037)
                     /locus_tag="ACHAY1_000005"
     CDS             complement(6864..7037)
                     /locus_tag="ACHAY1_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8592041.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            7332..7637
                     /locus_tag="ACHAY1_000006"
     CDS             7332..7637
                     /locus_tag="ACHAY1_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592042.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(7698..8735)
                     /locus_tag="ACHAY1_000007"
     CDS             complement(7698..8735)
                     /locus_tag="ACHAY1_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8592043.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(8851..9759)
                     /locus_tag="ACHAY1_000008"
     CDS             complement(8851..9759)
                     /locus_tag="ACHAY1_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8592044.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(9749..10168)
                     /locus_tag="ACHAY1_000009"
     CDS             complement(9749..10168)
                     /locus_tag="ACHAY1_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8592045.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(10169..10576)
                     /locus_tag="ACHAY1_000010"
     CDS             complement(10169..10576)
                     /locus_tag="ACHAY1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8592046.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(10563..10730)
                     /locus_tag="ACHAY1_000011"
     CDS             complement(10563..10730)
                     /locus_tag="ACHAY1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592047.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(10740..14630)
                     /locus_tag="ACHAY1_000012"
     CDS             complement(10740..14630)
                     /locus_tag="ACHAY1_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8592048.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(14627..15319)
                     /locus_tag="ACHAY1_000013"
     CDS             complement(14627..15319)
                     /locus_tag="ACHAY1_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8592049.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(15396..15797)
                     /locus_tag="ACHAY1_000014"
     CDS             complement(15396..15797)
                     /locus_tag="ACHAY1_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592050.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(15868..17928)
                     /locus_tag="ACHAY1_000015"
     CDS             complement(15868..17928)
                     /locus_tag="ACHAY1_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8592051.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            complement(18448..18588)
                     /locus_tag="ACHAY1_000016"
     CDS             complement(18448..18588)
                     /locus_tag="ACHAY1_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592052.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(18636..19016)
                     /locus_tag="ACHAY1_000017"
     CDS             complement(18636..19016)
                     /locus_tag="ACHAY1_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592053.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(19026..19616)
                     /locus_tag="ACHAY1_000018"
     CDS             complement(19026..19616)
                     /locus_tag="ACHAY1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8592054.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(19619..19960)
                     /locus_tag="ACHAY1_000019"
     CDS             complement(19619..19960)
                     /locus_tag="ACHAY1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592055.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(19957..20388)
                     /locus_tag="ACHAY1_000020"
     CDS             complement(19957..20388)
                     /locus_tag="ACHAY1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8592056.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(20381..20719)
                     /locus_tag="ACHAY1_000021"
     CDS             complement(20381..20719)
                     /locus_tag="ACHAY1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8592057.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(20716..21027)
                     /locus_tag="ACHAY1_000022"
     CDS             complement(20716..21027)
                     /locus_tag="ACHAY1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8592058.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(21038..22249)
                     /locus_tag="ACHAY1_000023"
     CDS             complement(21038..22249)
                     /locus_tag="ACHAY1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8592059.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(22253..23008)
                     /locus_tag="ACHAY1_000024"
     CDS             complement(22253..23008)
                     /locus_tag="ACHAY1_000024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8592060.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(22908..24236)
                     /locus_tag="ACHAY1_000025"
     CDS             complement(22908..24236)
                     /locus_tag="ACHAY1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8592061.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(24302..25036)
                     /locus_tag="ACHAY1_000026"
     CDS             complement(24302..25036)
                     /locus_tag="ACHAY1_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592062.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(25092..26657)
                     /locus_tag="ACHAY1_000027"
     CDS             complement(25092..26657)
                     /locus_tag="ACHAY1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8592063.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(26740..26883)
                     /locus_tag="ACHAY1_000028"
     CDS             complement(26740..26883)
                     /locus_tag="ACHAY1_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592064.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(26880..27056)
                     /locus_tag="ACHAY1_000029"
     CDS             complement(26880..27056)
                     /locus_tag="ACHAY1_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8592065.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(27040..27234)
                     /locus_tag="ACHAY1_000030"
     CDS             complement(27040..27234)
                     /locus_tag="ACHAY1_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592066.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(27236..27613)
                     /locus_tag="ACHAY1_000031"
     CDS             complement(27236..27613)
                     /locus_tag="ACHAY1_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8592067.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(27687..28922)
                     /locus_tag="ACHAY1_000032"
     CDS             complement(27687..28922)
                     /locus_tag="ACHAY1_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8592068.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(28922..29704)
                     /locus_tag="ACHAY1_000033"
     CDS             complement(28922..29704)
                     /locus_tag="ACHAY1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8592069.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(29719..30291)
                     /locus_tag="ACHAY1_000034"
     CDS             complement(29719..30291)
                     /locus_tag="ACHAY1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8592070.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(30425..30790)
                     /locus_tag="ACHAY1_000035"
     CDS             complement(30425..30790)
                     /locus_tag="ACHAY1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8592071.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCELCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(30958..31386)
                     /locus_tag="ACHAY1_000036"
     CDS             complement(30958..31386)
                     /locus_tag="ACHAY1_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8592072.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(31383..32735)
                     /locus_tag="ACHAY1_000037"
     CDS             complement(31383..32735)
                     /locus_tag="ACHAY1_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8592073.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(32735..33019)
                     /locus_tag="ACHAY1_000038"
     CDS             complement(32735..33019)
                     /locus_tag="ACHAY1_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8592074.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(33016..33225)
                     /locus_tag="ACHAY1_000039"
     CDS             complement(33016..33225)
                     /locus_tag="ACHAY1_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592075.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(33363..35606)
                     /locus_tag="ACHAY1_000040"
     CDS             complement(33363..35606)
                     /locus_tag="ACHAY1_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8592076.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(35599..35943)
                     /locus_tag="ACHAY1_000041"
     CDS             complement(35599..35943)
                     /locus_tag="ACHAY1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8592077.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(35936..36706)
                     /locus_tag="ACHAY1_000042"
     CDS             complement(35936..36706)
                     /locus_tag="ACHAY1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8592078.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(36917..38854)
                     /locus_tag="ACHAY1_000043"
     CDS             complement(36917..38854)
                     /locus_tag="ACHAY1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8592079.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(38915..39457)
                     /locus_tag="ACHAY1_000044"
     CDS             complement(38915..39457)
                     /locus_tag="ACHAY1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8592080.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(39459..40601)
                     /locus_tag="ACHAY1_000045"
     CDS             complement(39459..40601)
                     /locus_tag="ACHAY1_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8592081.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(40602..40916)
                     /locus_tag="ACHAY1_000046"
     CDS             complement(40602..40916)
                     /locus_tag="ACHAY1_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8592082.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(40921..41127)
                     /locus_tag="ACHAY1_000047"
     CDS             complement(40921..41127)
                     /locus_tag="ACHAY1_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592083.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(41224..41841)
                     /locus_tag="ACHAY1_000048"
     CDS             complement(41224..41841)
                     /locus_tag="ACHAY1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592084.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            42236..43186
                     /locus_tag="ACHAY1_000049"
     CDS             42236..43186
                     /locus_tag="ACHAY1_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8592085.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            43179..43625
                     /locus_tag="ACHAY1_000050"
     CDS             43179..43625
                     /locus_tag="ACHAY1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8592086.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            43628..45460
                     /locus_tag="ACHAY1_000051"
     CDS             43628..45460
                     /locus_tag="ACHAY1_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8592087.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            45476..45679
                     /locus_tag="ACHAY1_000052"
     CDS             45476..45679
                     /locus_tag="ACHAY1_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592088.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(45730..46866)
                     /locus_tag="ACHAY1_000053"
     CDS             complement(45730..46866)
                     /locus_tag="ACHAY1_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8592089.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(46856..48811)
                     /locus_tag="ACHAY1_000054"
     CDS             complement(46856..48811)
                     /locus_tag="ACHAY1_000054"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8592090.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(48804..51521)
                     /locus_tag="ACHAY1_000055"
     CDS             complement(48804..51521)
                     /locus_tag="ACHAY1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8592091.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(51522..51743)
                     /locus_tag="ACHAY1_000056"
     CDS             complement(51522..51743)
                     /locus_tag="ACHAY1_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592092.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(51892..52728)
                     /locus_tag="ACHAY1_000057"
     CDS             complement(51892..52728)
                     /locus_tag="ACHAY1_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8592093.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(52937..54313)
                     /gene="rlmD"
                     /locus_tag="ACHAY1_000058"
     CDS             complement(52937..54313)
                     /gene="rlmD"
                     /locus_tag="ACHAY1_000058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8592094.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(54424..55479)
                     /locus_tag="ACHAY1_000059"
     CDS             complement(54424..55479)
                     /locus_tag="ACHAY1_000059"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8592095.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(55489..56919)
                     /gene="gatB"
                     /locus_tag="ACHAY1_000060"
     CDS             complement(55489..56919)
                     /gene="gatB"
                     /locus_tag="ACHAY1_000060"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8592096.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(56916..58388)
                     /gene="gatA"
                     /locus_tag="ACHAY1_000061"
     CDS             complement(56916..58388)
                     /gene="gatA"
                     /locus_tag="ACHAY1_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8592097.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(58388..58693)
                     /gene="gatC"
                     /locus_tag="ACHAY1_000062"
     CDS             complement(58388..58693)
                     /gene="gatC"
                     /locus_tag="ACHAY1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8592098.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(58799..60835)
                     /gene="ligA"
                     /locus_tag="ACHAY1_000063"
     CDS             complement(58799..60835)
                     /gene="ligA"
                     /locus_tag="ACHAY1_000063"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8592099.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(60852..63086)
                     /gene="pcrA"
                     /locus_tag="ACHAY1_000064"
     CDS             complement(60852..63086)
                     /gene="pcrA"
                     /locus_tag="ACHAY1_000064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8592100.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            63241..64800
                     /locus_tag="ACHAY1_000065"
     CDS             63241..64800
                     /locus_tag="ACHAY1_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8592101.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(64911..66842)
                     /locus_tag="ACHAY1_000066"
     CDS             complement(64911..66842)
                     /locus_tag="ACHAY1_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8592102.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(66860..67774)
                     /gene="pfkB"
                     /locus_tag="ACHAY1_000067"
     CDS             complement(66860..67774)
                     /gene="pfkB"
                     /locus_tag="ACHAY1_000067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8592103.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(67771..68523)
                     /locus_tag="ACHAY1_000068"
     CDS             complement(67771..68523)
                     /locus_tag="ACHAY1_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8592104.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(68682..68891)
                     /locus_tag="ACHAY1_000069"
     CDS             complement(68682..68891)
                     /locus_tag="ACHAY1_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592105.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(68902..70230)
                     /locus_tag="ACHAY1_000070"
     CDS             complement(68902..70230)
                     /locus_tag="ACHAY1_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592106.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            70366..70521
                     /locus_tag="ACHAY1_000071"
     CDS             70366..70521
                     /locus_tag="ACHAY1_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8592107.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(70595..71182)
                     /gene="yihA"
                     /locus_tag="ACHAY1_000072"
     CDS             complement(70595..71182)
                     /gene="yihA"
                     /locus_tag="ACHAY1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8592108.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(71265..72515)
                     /gene="clpX"
                     /locus_tag="ACHAY1_000073"
     CDS             complement(71265..72515)
                     /gene="clpX"
                     /locus_tag="ACHAY1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8592109.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(72762..74051)
                     /gene="tig"
                     /locus_tag="ACHAY1_000074"
     CDS             complement(72762..74051)
                     /gene="tig"
                     /locus_tag="ACHAY1_000074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8592110.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(74205..75137)
                     /locus_tag="ACHAY1_000075"
     CDS             complement(74205..75137)
                     /locus_tag="ACHAY1_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592111.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(75294..75953)
                     /locus_tag="ACHAY1_000076"
     CDS             complement(75294..75953)
                     /locus_tag="ACHAY1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8592112.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(75956..76981)
                     /gene="sppA"
                     /locus_tag="ACHAY1_000077"
     CDS             complement(75956..76981)
                     /gene="sppA"
                     /locus_tag="ACHAY1_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8592113.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(76994..77284)
                     /locus_tag="ACHAY1_000078"
     CDS             complement(76994..77284)
                     /locus_tag="ACHAY1_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592114.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            77694..79499
                     /gene="glmS"
                     /locus_tag="ACHAY1_000079"
     CDS             77694..79499
                     /gene="glmS"
                     /locus_tag="ACHAY1_000079"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8592115.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(79571..80926)
                     /gene="glmM"
                     /locus_tag="ACHAY1_000080"
     CDS             complement(79571..80926)
                     /gene="glmM"
                     /locus_tag="ACHAY1_000080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8592116.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(80950..82119)
                     /locus_tag="ACHAY1_000081"
     CDS             complement(80950..82119)
                     /locus_tag="ACHAY1_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8592117.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(82116..83003)
                     /gene="cdaA"
                     /locus_tag="ACHAY1_000082"
     CDS             complement(82116..83003)
                     /gene="cdaA"
                     /locus_tag="ACHAY1_000082"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8592118.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(83192..86872)
                     /gene="nifJ"
                     /locus_tag="ACHAY1_000083"
     CDS             complement(83192..86872)
                     /gene="nifJ"
                     /locus_tag="ACHAY1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8592119.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            87070..87591
                     /locus_tag="ACHAY1_000084"
     CDS             87070..87591
                     /locus_tag="ACHAY1_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8592120.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(88290..88855)
                     /locus_tag="ACHAY1_000085"
                     /pseudo
     CDS             complement(88290..88855)
                     /locus_tag="ACHAY1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(89034..89690)
                     /locus_tag="ACHAY1_000086"
     CDS             complement(89034..89690)
                     /locus_tag="ACHAY1_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592121.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(90258..91193)
                     /gene="whiA"
                     /locus_tag="ACHAY1_000087"
     CDS             complement(90258..91193)
                     /gene="whiA"
                     /locus_tag="ACHAY1_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8592122.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(91227..92225)
                     /gene="yvcK"
                     /locus_tag="ACHAY1_000088"
     CDS             complement(91227..92225)
                     /gene="yvcK"
                     /locus_tag="ACHAY1_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8592123.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(92222..93106)
                     /gene="rapZ"
                     /locus_tag="ACHAY1_000089"
     CDS             complement(92222..93106)
                     /gene="rapZ"
                     /locus_tag="ACHAY1_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8592124.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(93241..93972)
                     /locus_tag="ACHAY1_000090"
     CDS             complement(93241..93972)
                     /locus_tag="ACHAY1_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8592125.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(93976..95241)
                     /locus_tag="ACHAY1_000091"
     CDS             complement(93976..95241)
                     /locus_tag="ACHAY1_000091"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8592126.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(95504..98320)
                     /gene="uvrA"
                     /locus_tag="ACHAY1_000092"
     CDS             complement(95504..98320)
                     /gene="uvrA"
                     /locus_tag="ACHAY1_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8592127.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(98330..100324)
                     /gene="uvrB"
                     /locus_tag="ACHAY1_000093"
     CDS             complement(98330..100324)
                     /gene="uvrB"
                     /locus_tag="ACHAY1_000093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8592128.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            100583..102085
                     /locus_tag="ACHAY1_000094"
     CDS             100583..102085
                     /locus_tag="ACHAY1_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8592129.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            102087..102824
                     /locus_tag="ACHAY1_000095"
     CDS             102087..102824
                     /locus_tag="ACHAY1_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8592130.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        28534 a        22908 c        18257 g        33377 t
ORIGIN      
        1 agaattagca ggtaatttta agtagaattt tcctttactt tccacaaaat aataatcttc
       61 caaaacgatt gactgaccat cttgctcaac atctttagca gatattttgc tgcatggaat
      121 aatctgaata cgttttaatg aactaacatt cttactattg tcatctatcg tagataatac
      181 cgcagttttg atatcatcaa tctctttttt gaaatcctca gaataatctt ccaataactc
      241 aaatgtttcc aattttaaat ttgttttgaa gcgttcaacc ccaagccatt catatatcgt
      301 agttgtacta gctttttcag gcacgtaaat aaaacgcatt tgtttttcta ttgatttagg
      361 cagtcgctta tcagcatacc ttaattcttt gtttggataa aagaagccat ctttggctaa
      421 gactgataat ccttcggtag aataagtagg attcattcct tttttatttc ttataagttc
      481 attatataat ctgcgactta tttcgccttt atcgaatttt ggtaactcaa ctaatatagc
      541 tcttatttca tcatccgaaa acatcgccat attttttctg aaatccaact gttcaacaat
      601 gcgttgcccc aagagttcca ttaagctttg ttcagaaact ccatacaatc ccggaacgtg
      661 tgcctggagc ttttcatata gcacaatttg tctcgggcta tatttttctc catcaatctt
      721 aatccatgca gttttattca aaatgtattt cacatactca tttgaattaa agtacaatcc
      781 actccagttc gattgctgac ttgcacttga ttcttttttt accgaagata ataaatctgt
      841 caattcttcg tcttgattga gccaattgac aatgtctttc gtatctaatt tgtctattaa
      901 ttcttcaaaa ttactgatgt acttagaagt taaataatta atattcgtaa attttatttc
      961 attcttatat ttgttataaa taaacttttt aaatccaaca tcatttttta aagaatcgtt
     1021 atcataaatc ttaggatatt tggaaacacc acatcgaaga ataaatgaca gaaaatctct
     1081 ctcctcagca aaactagagc aatctatttt tttcaatgcc gtgtaatctg tatcttgaaa
     1141 aagcttatct cctaattcat tgccatactc ttttccaata tacattttat tggaaggttg
     1201 aacgtcacca gcaacgtttg gaagattaaa tggaacttta gacagctcac ttccagctga
     1261 tagtttttca ttgtagttat cataaaacca attaatgaaa ctgatagatt tttcaacagt
     1321 gtcaatttgt cgattaatcg ttccaatgat caaatctgaa ctgctttgct cagtgaattc
     1381 aattgcaaga tatttttcac tgaatgcatc tagaatacgt ttatctcctg tacgttcagc
     1441 tttgtatgca ttagctgttt cttcccattt atttttccaa cttgatgaag tcttaatctg
     1501 ataaattaac tcatcaacat atgtttgttt caaaatgctt aaactaaccc aagaagacag
     1561 ctcttttgga agcacagtaa cacctgtatc ggtcggtaag aataccttat ctgatttcat
     1621 caaccaattg tcactattgt cctttaatag cttagggaca atcggagaac ttttatattg
     1681 ctcactccac cataagaaca cttttgtcct tattctattt tcccaagaag atgaaagaat
     1741 gttgatttta tcagatagtt catctacaac aaacgacagg ccagtgtatt cattataatt
     1801 agcaagttct tgtactagtt ggtcattttc gtttgtttct aataattgca acaaggtgga
     1861 aaatgtctca cctattagct cctcagggaa ttcttgaagg aaaagttttg gattatcttt
     1921 gatagaaata tattgccaat tgacagtcgg tagtatttgg gcattagata aaatacccaa
     1981 gaattcgttc attaagtcaa atacatctaa gtcccatagt ttattctctt taaaaggagt
     2041 taccattttt aatgctatgt cttcatattc aggcttagat aatttttctg ctacttcaga
     2101 aataaatttg agcacctcaa taaatatttt cttatttatt tgttcactat catccgggac
     2161 gtcatcacgt gaattatttg ttataaacgg tgcatttata agtgcatgaa ttggtgtacc
     2221 aaaattacgt actggaaagt agcaatatag tttttctttg gagtaatcgt aatcagaatc
     2281 tttagaaacg gctagtgcaa ttttagcaac tttatctcct atcatagatt ctttgtcaaa
     2341 aacaaaatat tcttcagacg gtgattgacc agtaacatca cttttttcta tcaatacatc
     2401 atttccatcc acaattttct ctgatatttt ttcatcatca gttgtttcaa taacaatttt
     2461 tgttatattt ggtaaaaata acagtgactt ataaaatggc tgctgaagtt gtttagaaac
     2521 actcgtgcct ttacttaaat tagtttcttt tagttttact cgaattaatg tatcaaaacc
     2581 agaatcaatt ttgtttattg gttgaggaca gttcattatt ggaaaacata aatcataatc
     2641 ttcttttttc caatttttct tctgctcaaa aattaattca ctgaatttgt cagaatctct
     2701 tgatacatac tgctcaaaaa gcttttttgt gaattcttca ctaaatcgaa tagcaaattc
     2761 tcccgaatac agttcgaccc actcagcatc gtttagcaag gaacgaaaac cagtcccctt
     2821 atttccgata ttttgtgatg atttattact cgctcttcct aagcacaatc tttcaattgt
     2881 atcttttgaa aatgaagtgc ctgtattccc aacttccaag atattatttt tatatgttat
     2941 ctgaactttc acttcgtcat tgctagcatc attttctgat tttatatctc cataggcatc
     3001 gtctacattc tgtaataact ctaaaatctg acgcccatta taatcacgca tcaattcata
     3061 ttcttcgctg caggcctttt caagttcatt actcaactca gaatccgtat atttacgaat
     3121 aaaaatatcc cttaattcat caatggtatt ccattggctc acagaacttc gcccccagcc
     3181 tattttttac ataatatatt atatcatcaa tcactttatg aaatccaatc aataaagacg
     3241 ttaatatggc tacccacatt aacgtctagt cctatctatt caaattaaat ttctacagtc
     3301 actctcaccc cacttttgaa atcaacaaca atgttctttt caaaaatggt gaccttctca
     3361 atcaaccttc gaacaagcac ttcattatac tcattgatga catcgtgttc ttttacaaag
     3421 actaccatct catcaacccg ttgttgcaaa ttagtattcg ctgctatttg ttttgcaacc
     3481 gcatctcgtt catctcttag tgaaaatatt tggcttgcca attcatcgta accctgaccg
     3541 ccaactgcca ggctaataat ttcagcttca agctctttca actgactgtc tagttctgcc
     3601 aatcggtcag ttaatccatc agtcataact gagttgatat tgtcatttaa ttgctgaaga
     3661 aaagcatcac gatcgattaa cgtttcattg attgccttga taacagctcc atgcaggtct
     3721 gtttcgtaga ttgttcgacc cgtgcaatta tgttctcgtt tatccactct ggatttgcat
     3781 cgccacacag ttcctcgatt cttccaatga actctgcggt aagcattgcc acagtcaccg
     3841 caaactgtga taccagataa cgcatacttc ccagaaaagc actgtgactt tttgtttttc
     3901 attgatttac gccggtctag ttcttgctgg gcttgcatga agatatcttt tgggataatg
     3961 gccggatggc tatcttcaac ataatactgc tgagcgtaac tcccgtcatt cttcactcgt
     4021 ttctttgtca agaaatcggt agtatatgtt ttttgaagca gcgcatcccc catgtacttc
     4081 tcgtttcgaa gaatcaatct gactccttct cctcgccaga gctttcgctt ccctcccgtt
     4141 aggatgccat cagccattag accattagca atttcttgca aggaactgcc ctcaaggtat
     4201 tcgcggaaga tgcgtttgat gacttctgct tcctttggtt caatcaccaa gttaccatct
     4261 tcgtccttag tgtacccaag aaaatgattg tggttgatac gtagctgtcc ctgctggaaa
     4321 cgatactgta cgcccatctt ggtgttttga cttagtgact ggctttcttg ctgggccaga
     4381 cttgccataa tggtcaaaag tacctcaccc ttcgcatcca ttgtattgat gttttccttt
     4441 tcaaaaaaca ctgcaatctt cagttctttt aactgtcgta tgtattgcaa gcaatcaacc
     4501 gtattacggg caaagcgtga aattgatttt gtgatgatgt actcaacttc gccgttttga
     4561 cacgcctcaa tcatccggtt aaaaccgtca cgctttttcg tcccagttcc tgaaatccct
     4621 tcatccgcaa atattcctac aagctcccaa tcgggatttc tttgaatgta gtccgtatag
     4681 tgttgaacct gtgcttcgta agaactcgcc tgttcctcca tttcagtaga aacacgacaa
     4741 taggctgcca ctttcaactt tggtttctct tcggtattct ctgctgccca ttgtctaggc
     4801 ttcattggaa taaccgtcac ctgttttcct gccatttcac ttaacctcct tgatttttga
     4861 atatgcatat tgcgcttgtt tgaatgggtc agaatacttt tcggtaatct tgccagattt
     4921 aaaatgagtg ggtattacct tcgtatctgg cactttgatc tccttgcgtt ttttaaagaa
     4981 cttaattctt cgttcttgct ccagatccgc ttggaagaaa atatcctcat caatgatttt
     5041 tggatatatc tcatttccta agtaaacctc attcatcaaa atacgtttca taaagggatg
     5101 cggcttgtta atgccagagc gtttgccagc ttcttgtaag ccaacaccct caaggtaaat
     5161 attgaagaac tcacgtattt gttgagcttg ctgttcatcc acgttgattt ctccattctt
     5221 aatgacatac ccgtatggaa ttgtattgtt cattctggta acctctcttt caaaagtagt
     5281 ccgcacttaa gttcaaatcc gacttcttgg cggttgtaaa ctgtaatgct gtttgtgaac
     5341 ttcttgaata aatcttcatc ccagtccgtc aacatctctg ccatccgcgc gaatttgtat
     5401 aaggcttcta gttcaatttg ctgagtgctt ttgccgttga ctgactcaat gatgaggcgc
     5461 tgcttttctt gtgcgacagc cctctcttgt tgaagtttgc tttgttcttc taaagacacc
     5521 ttcgttgata aaatactgga agcagtcaaa cttgccagaa cctcaagctt ttcttgaatc
     5581 ttgtcgattt catcagcaag cacttcgagc ccttcctgat tcgcacctgc tttattcaaa
     5641 gacttcataa aaggttccag aaccaacttg tgtccgaaaa tcaacttatt caacatggtc
     5701 aaataagcaa ccttcaaatc ttcatccgta attggtaatg catcgcagct atctttattt
     5761 ttaagatgcc ccgtacactt ccactgaacc ttcttcgtat tacctttcca ataagtgtga
     5821 acccgcttga atttgctgcc gcatttatca cagatgatgt tgcctgaaaa tgcgtatcgc
     5881 ttggtgtact ttttcccgtc accagtattc cctttggcct cagcttttct ccgaatgagt
     5941 tcttgaactc ggtcgtaatc ttctttagat actatgggtt cgtgattgtt tttgatctgg
     6001 tacattggca attcgccctt gtttatgcgt cgtttgtact ggtcatcaac aaaggtttgt
     6061 tgcatcgtca aagtcccggt ataaacttca tttttcaaaa tacttctgac tgatgttgct
     6121 tgccaatttg ccccacgttt agtgaattgt tttccatcgt ttaaccgctt ggcaatcatt
     6181 ccagtcgaaa tgccatctaa tgcccactgg aacatttgtt taacaacttt tgcttcagct
     6241 gcaacgatgt gaaaatcata gcctttccgc ttgtagccat acggcgggta agctttgaat
     6301 ttccctttag acatccggtt tcgaatcgcc cacttcaagt tttctgaatg ggaataggat
     6361 tcactttctg ccaagcttga taaaattgaa agcaacaact cgctttccat atcaccagta
     6421 ttaatgtttt ccttttcaaa aaagatggtg acaccaatat cagttagctg ccgaaccaga
     6481 ttcaagcagt caactgtatt tcttgaaaat ctggaaatag actttgtcag aatcaaatca
     6541 atctttccaa gctctgcatc cttaatcaat cgttttaaac catcacgttt tacagtcttg
     6601 gttccagaaa tcccttcatc ataataaaca ccgacatagt cccattgttc atggttcttg
     6661 atgtgatttt catagtgagt aatctgggtt tgtaaacttg ccagctgttc attacttgat
     6721 gttgatacac gagcgtatgc tgccaccttt agtttggaag cttccggcaa ctctggacgt
     6781 gccaatttat gtatcgtttt catcagctca actcctttcc attaggtgac atgttagctc
     6841 taaagtgccg ttatatcaac gtttcagacc attatccttg ataaaaaagg attgaatgtt
     6901 tcccggtttt tattgtcgat tttgacgtat tcttccaggt tgattttacc ggtaacaagt
     6961 aagttcagaa ggatctgctg tgaaatgtaa taggctgctt catctgaata gttaatttct
     7021 gttttcttgt tagtcattgc tttcgcctcc tcaagataca ggcgaagaaa atccatgaaa
     7081 gtaaacccaa tcacaaattt tcctttgcct ttcatccata tgcgaaagaa ataaccgact
     7141 cgaacccaca aaacgaaaaa agcctgcaag aatgaaacga atcactcttg caggcttcta
     7201 agtttgttta tatatagtag gaattattct ttgtaaattt tgacttttta cttcccaaac
     7261 atcaaataag cctttacaag cttcatccaa taaataacaa tataaatatc aattgtggga
     7321 aggagaaagt tatgagtagt tttgatatgg aagggttaga aaaagaatat gaacattttt
     7381 gcaaatctgc gacaacaaag gaactggagg aatttgatga aagcttcaaa caagaatttg
     7441 aaaagtgctt taaaaccata gaggctgaag aagctttata ccagttttta caagccaata
     7501 taaacgcgat gaataatcgt atggaatcaa tagaatcgcc tcttttctgc atcaattaca
     7561 aagagcaggt atttcagtta agaaatcatt taatcctaat gcaaaatgct gtaaacacct
     7621 accttaaaga aaactagacg aaaaaaaggc cccacaataa aacttgtgag gctgttttga
     7681 attataaaga cgtcaaacta cttattgaaa attttgtagc tcgttggaac catcgtgaag
     7741 ttttcttcag taattgggcc atcataaatt ttgattttgc aaacttttga cgccacatca
     7801 aggtcgtgaa caaagtaaat tttatatggt gtatctccaa gggaggcagc aacctcatta
     7861 gtactcattt caaatccgtc agagaagttt gtttttgaag ctttcacttc aatatggatt
     7921 tcattacctg attggtcaaa cgcacaaata tcatagccat gaccatctcc ttcagtttta
     7981 gaaacatgtt caggaagttt tgttttgttc ttttctgctt cctgaatgag gaaatcaagt
     8041 acgatttctt cgcctagagc acccgttttt tgttttgcct tgttgacacg ctcccaatca
     8101 gtatggcgtc cagtgaattt tcgtttctga gtcccattcg tgcgatttgg tcccacttca
     8161 tcaacaagaa cagtaaatcc ttttgaactt tcttgctcaa tttcttcaac ttcctcgttt
     8221 gtaagatcca gttcagacgg caacgattgt gtatcatcga ttgggataag atgacgattt
     8281 tcaatcaatg caacaaaatc ggcataggta atttcattca ttccatactg atcccagaag
     8341 taggcccatg aattcggctt tttacgcttc tttttgctag gtgtattcca atcgaagttt
     8401 tcaataaatg aatcaccttg tttaattgcc ggttcgattg taaatgcgtg tgtaattact
     8461 gccgtaacat ttccagcttc atcaggttca gaaatgctct caacgtatcc gcccccgtaa
     8521 atttcccact tacggtcttt agtaattttc cccggatgac gattcaaaac aaccgcgccg
     8581 gctttcaatt taccatattg gcgcatgttc caaccgtaac ttttagcatc atcagcgtat
     8641 ttcaactgac catcatcttc tgttttcccg tgatctaaaa tgaaagcttg ttggctttct
     8701 gttggttctg gtaacaaatc tttaaacgta accatatcga tacctctttc tataattcaa
     8761 tcggaacaaa tttatgttct ggttgaatta ataataacac gttcgatatt ttaagtcaac
     8821 aatccggaca taattttgtt ccgattaaat ttatgaaatc aaaatataat cgtcagattc
     8881 cgtcttacca gtattcttac cgacagcaca atatccaatc gggcaacgag cccataccca
     8941 accatccgca atcgtcatct tctctgactt cggaagcgtc caagttgcgc ctttattgta
     9001 tgaaccaaca accgcaccac ttaaacttgg cttgttgcga acgttgagac cactcacttg
     9061 aaccacaaga gttttcacgt tggatgttga tgacccagat ggataaactt gattaccgtt
     9121 ctggtcgaac accttataac cagaatggct atctgcagcc cgcttcgcat tgttcaagtc
     9181 cttataggca ccaatttgtg acttggcgtc agcccatgat ttgcgaacac gatacagacc
     9241 actagctggc gtgcttgggg ttgatgaacc attattaatc gcattggctt tatccgcaat
     9301 ctcctgcagt ttaccaagca actgtcccgg acatgatgtg gccgaaaaat gtgagtgagg
     9361 gaagatattc ttgttcacca ccaatttccc tagaccataa cgtttggcaa tatctgcaca
     9421 caactttgca cttgcatcaa tagttgcttg attgataccc cagttcggtg atccagtgct
     9481 attgcagtgc tcaatcccga tacttgagat attggcgttc caattaccag cgtgccaagc
     9541 tgtgtttcct tcgtccacgt atgctcgaat ttctccattt tttccaatac cgtagtgtgc
     9601 tgacgcctca cgcgttttcc aaatatcagg aacaatatca tagttggtgg ttgccatgtg
     9661 atgaatcact atcttatcta ctttggcacc actacgacca ttcgagtaat ggctaaatga
     9721 ctgaaatcca tattttgttg ctagattact aattcccatt ttctgtttcc tccttctttt
     9781 cgtgtaactg ctgcaagatt tcacgcaact tttccggcac tggcagtcca atcgccacgg
     9841 cattctccag cagtgaaatt ccttcgtttg agatatagaa gaaaatgacc gcatcacgca
     9901 gtacatttcc acctttgatg acttccacat caagcatatg agccacacca accaaaagaa
     9961 aaatcatgat tttcttagtg atgccctttg ccccaatctt gctggatagt ttcttttcgt
    10021 tgattgcacg aagcagacca gttacatagt ccactaccac aaaagttaca agcgtataaa
    10081 tcaaaccatc aattcctccc acataccatc caatcactcc tccaccacct gcgatggcga
    10141 gttgaatcca attccacact tctttcattt acaaatcctc caattgttct aaaattaaat
    10201 cgagcatttc agcatctttc cctgaaatgg tgacttcact attttcaaat ccagcaacca
    10261 ataaaggcag cttgtcttga agttcatcca cgttgatgtg ggtttcttcg ttgagcaagt
    10321 ctgccttttc tttgtgatat tcagttgcgg tttctgggat gagtgaaaag gcaccgtcat
    10381 gttggaccaa attgccgtgc tcatctttct ttccgtattt ttgaagcaag tctacctcgt
    10441 cttgatagag ttcatcaacc ttcacctgga tgagttttgc aagcttcgtt cgcactcggc
    10501 taatctttgg tggtaagtca aaacctttta agaaactcat cacgtccacc aaatcttcat
    10561 ttctaatttt gagcattatc tatcttctcc tttaaatcac caatctggac acaaagatca
    10621 tccacgacca tcacaagcaa atcaaccata tccgtcccaa ctttcattgg ctttggaata
    10681 tctgcctcga taggttcagt tactatcact tcttcttttt cttcgttcat ggcggcctcc
    10741 taactgtatg tggtcattga attaaacgac tgcggattgt accaaccact cgctgtaccg
    10801 ttgctactga aaccggttgg taaagcgatt gttcgtcccg caagtttggc acagatttca
    10861 cggatgactc caaagtcaac ccacttccca tcatctccga gcatcagatt agaaccgttg
    10921 aatgcaatac cagcgctgcc ggaacttgag ccaatcttag tataaaggga actactaaat
    10981 gtaatcccac caacagatat ttgatgttgt gtccctatac gaagcattga tggtaaataa
    11041 atgtcgtcgt caaagtggaa ccccttctcc ataccagact gcaatgttct cgcataccaa
    11101 gaaagcttct gggtgtagac actcgcagag gaggaatctt tatgagccca tgacatatac
    11161 tctgttccat actcaagatc aaataccaat ccacgccaat cagagtgacc actgatgtta
    11221 tttgcaccaa ttgttccgac tgtcgttcca ccacgtcgaa tggtcattcc ctgcgagttt
    11281 atttcagtca caagagtgct tcctgaataa tatccaatcg tagttcctgt gattcgaatg
    11341 gacgaactcg cagtcaatgc tgttaggata ttagcagcaa tcttatcagc cgtaatcgag
    11401 ttcgcgccaa tacgggctgc cgacaaagtt cctgtggtaa tcttcgccgc atcaatgctt
    11461 gcaatttttg cactttgaat tgtggcatct gcaatctttg cgtttgtgat ggccgcattt
    11521 tggatcatgg cagaactaat cactgcacta tcaatggttg tttgaccagt gatgtgcacc
    11581 ttgtttccag caatcaaaat actttcaggt gagatattga tttgattaat cacgtctcct
    11641 ttaccaactc gtaaattgat taagtctttc tgcatgagaa ttgctgaata taatgtgttg
    11701 gcattggtat agtcttgcct tgtggtcgta ggataaatat ttccttcata ttcatcgggt
    11761 ttgatgctcg tgttcactcg gactcgcatt ttcaatgtgc cactcgggac tgtaattgct
    11821 ccgctcgaac cagtataaga gatttgatga taacttgaac catccgacgg gtttagagta
    11881 aaaggtgtgt tgcttgaatt ataaatccca gcatatgtgc cagtcttaac tgaaaagaaa
    11941 gctagataca cgctgtcagt agtaatccct gatatctgct cagccgttaa cgtccaagaa
    12001 taagtggcat tgctgctact tgtaacggtc gaaatctgtt cccaagcacc acctgtataa
    12061 agcaagacaa tattatctga tgtatccttg cgaccattcc acgaaattac actcggagcg
    12121 actttgcctt ccaatggcaa tttgtaaatg accagataat ctcctgaatt tgaatagggg
    12181 tttgttgtca cgccacttgt aatactcatc gattcaacgt tcttacttac tagataactc
    12241 gcaatggtcg cattagtata agttcccgcc gttagggttt cactaaattg ttttgccgtt
    12301 gggacaaaat caatgtaagg taaatccatg ccgtagtaat gatagtagaa agcagcttga
    12361 gttccgtcac gcagttgccc gatatactta tctcccactt tgacatcaag gaactcactt
    12421 cggagataat tggttgcgga cacttctgaa ccgtcagtgg tactcaaact tccttgttcc
    12481 cattgattga ctttaaatgg aacgccgatt gcttctttgg tttctgaaac acttaacagt
    12541 aaggcatcag tggtcgctgt aagttttacg acgttactta tctcggataa atcaacatag
    12601 ccatttgtca catccgtttt caacatatag caactgatgg tttctggtgc atcactagtt
    12661 gttaaacgaa tgcgaataaa agcggcactg cttggaacag taagcggtgt ggtggtgttt
    12721 ccaaattggg tgtacgataa aaaggactta tcctctttgt aataaagcag ataagccgtt
    12781 gcgtaagtag agtaaagttt cccacccaca aatgtttgaa gtagatactt ttcgccattt
    12841 ctaactcttg taaaacctga gcgaacgtat ccaccatagg tattttcttt tccatcattt
    12901 gtattcagac caccaactgc ccactgcaaa tctgcaatgc tcataccggc taaaacattg
    12961 accgctttat ttgttttaga aagagatagc tcaaatctat cagcattttg ggtgatggtg
    13021 ctttctgcag ttgtaatacg ccctgtcaga ttatcaaccg tcgattgact tgccttacta
    13081 gcaatctgcc cagcttgcac tgtcagagtt gattcagctg tgattactcg tccttcaaga
    13141 gttgacatac cactcgccac taatgaaatc ttgctagtat tctgttcaac ggatgtagta
    13201 agcgtttcaa cacttgcacc tacactttca gctgcattca aagcattcac agcatcgtta
    13261 aatgcttgat tggccttttc agtggcagta ttggcatcct tttgtgcttg ttccacggct
    13321 tgctttactt cttctgccgt catatctgtc ataagtggat accactgagt gacgccatca
    13381 cggatttcat acacccacat ttcaaacttg tcaccatttt ctttgtacca aacgtcaccg
    13441 agtttcggat tatttggagt tgtcgggcca tagaagttgg tgtttttacc atttgccgaa
    13501 ctcaatgcgt aattggcatc gtctttggct tgctccgcct tgttgtccac ccggttgatg
    13561 tccttcgcca catccgtgaa ttttggcgtg atgtttccga gtgttactga gatatacgct
    13621 ttcagaaatg gatcgtattt gtatgagacc attctagctg tgatgttcaa tccatcttcg
    13681 gcatgaataa ctgaaacagt atcacccaaa ttaatcgttt caagctgtgc aaaatcttta
    13741 tattcttcag ttcgctcaag cggggcaaac tccacatcat aggtggcttt tggcaaatcg
    13801 accttgttag ctgagtattc taacccagcc aatctgcgaa gttcatcata ggcctcggct
    13861 tgagtaaact catcctcttc atcaccaact ttgacgttgt cgtacttgat gactttgatt
    13921 ttaggctgga cgtattggtt gatgtttgga ctgtccacgt atttttcggg aagaaatagt
    13981 ccatcatacc cttgtggcat aatgcgagta acaattccag agtaatcaac atcagcttta
    14041 tatcctgtca gattcttctt gtctcggatt tgtacgccgt tgttactgcc acgagtcgtt
    14101 aacattgaaa tcgtgaagtt atcgcgtaca atttcaccac cataactgct ttgaaaacca
    14161 ttctctaggt cactatcaag caacacctct acaggattta gtcgcaccaa gcggacatta
    14221 ttgaccgcac tgatgtttga tgtaccggta aaacggtgca tgaattgagt tgcaccaagc
    14281 agttgtgtca aagctcccga cccgttcttg ttgacgatat aagtatcttc aataaagttt
    14341 gtggctaggt catagaaaat atgatgagct actatgtgca aaaaaccacc aagtgcatca
    14401 tctcgttcaa caatcctgaa aagctggtcg gacagttctg gtactggtgc tttcacaatc
    14461 atttctggca acaattcctc actgtgtgga gcatgaattg gataatcgaa ttcaaaggaa
    14521 aagagtccgt tcaattcctc actcaccacg gggttcagaa tgttgccatc aaggacagcg
    14581 agaccgttat gattgaaatt tgattctgtt ttgttgtaaa gcgtaatcat aagtatcgcc
    14641 acctcggttc tatttctact ttggttatac ctgtaccaag agtcacagta ttactgccga
    14701 ctcgaaactc aggaaagtca cctgtcatgt attgattggc tgccacatcc ccgtaaaagc
    14761 attctttcaa atccgaatct agtgtcaggt tgccattcgt cagatttaac acgataggct
    14821 tgccatttat cgtgagtgtc ctagttccat tgccgtagac cttaatcttc ggcaatgagt
    14881 aaatgttgcc gggattattg accgttcctg acgtggttcg attgatagtc gctacattgg
    14941 tcttataacg aaaaggcgaa caggttaact ccatttcaaa ctcccacatg gaagataaaa
    15001 tctgagttag tccgctcgcc ttagcatact tgattgggta aaacacgttt ggttcatttg
    15061 aaaatgcgat agttttagcg tttaggattt gtggcaaaat tatctgccag ctagtccaaa
    15121 catcagattt ccaaatcact accttaaaac cgaaagtcac atctttccag cctttcagaa
    15181 tggtcaaatc tccttcacgt ccatcaatct caatactatc aatgacacgt tccgtcactg
    15241 gaataactgg cacttccgta attcgcaaac ctaaatcagt ccgtgaatta attgttccgt
    15301 ctaaaataaa tgcgttcaat tgaccaccgc ccatctaaat aactaaaaaa ggaccagaca
    15361 acaatgtcta atcctaaagt ttataatcta tcatcctatt cggttgtttc ttccagccct
    15421 attttcttgg taatcgctaa atacgcgtat acaaccagca cgccggaaat tccatatatt
    15481 gcatcgatta gatctggtgt attcagaaaa gtcatcatcg tttcacatac aatatttgca
    15541 acaatcagac cagctcccat cgcagtaaga acccttaaat tcgttcccat aagcaaccaa
    15601 agatagccta tcaccaaata gaaaagaaag cttaatgcga agtttgaaat gtgagaatta
    15661 atctgattgt tgatgaatac aaaaccatga tttatgccgc tataccatct tactgccgtt
    15721 aaaaataatc caatagcaag cagtagaata tatcctatca gtgcaggttt ctttgcttta
    15781 aatttatgct tcaacatatg attaccacca tctcttcagt aaacactcgt tagttatagt
    15841 atacagtcaa aatgtgaaaa tccaacacta atatgctaca cgtctacgat tcaacgtcgc
    15901 caagctctta tcaatttctg gagcaagttt gccgaccaat gttccatcat tcaagacgac
    15961 tttaatgtcc attgctttaa gtagcgttgg caacatttca ctcgtgatgt ttgttaaggc
    16021 atcaacctta gcacctaaat caagcatcga gaatgtgcta tcaacggcag cgtggatatc
    16081 ggtatcaata tcaaagcttg gcatataaag ttttgttgga atagctttct gcatcgtttt
    16141 agaaacacca gtcatttcct tatcaaaacc tacgccaatc ccttgtgcca tgtacttccc
    16201 gatttcatca cgaaacagtg ttgatggtga atgaatacca aagaagtcct tgatgttgtc
    16261 tactacaccg ccaaagaatc ctgaaatctt gttgcgtaac caacttccag catcagcgat
    16321 accattccac aatcctgtaa tcaagttctt cccgatagtt cccatagttg aaacatactc
    16381 accgaaagca ttcgcaagcc ctgtgatgat ttgcggtact gctttgacaa tggcagaaat
    16441 gattgctggt aagttttgaa ctaatgccac aagcaacttc acaccagcat tgatgatttt
    16501 gtcgatgttg tttaccaaag ttcctgttat tccgttgata atttgcggca tggcattcgc
    16561 aatcgttgtg ataatttgcg gtaacgcacc aatcaatgcc acaagtaact gaatacctgc
    16621 gtcaatcaac tgagggattg ccccaatcac ggcattgatg atattttgaa taatcactgg
    16681 aatagctgca acaatcgcat tgatgatatc cggcaaagct gtcacaagtg atgtcaaaag
    16741 ttgaatacca gtctgaatga tttgtggaat agcactgata ataaagttca cgattcctgt
    16801 aataatctgt ggcagacttt caatcagtat tggaagtgct gtgataagtc cttgggcaag
    16861 tcccatgata agttgtaaag cagcatccaa cagtaatggc aagttttcaa tcaagccgtt
    16921 cacaatcgtt atgattgcct gtactgtaat tggaataagt tctggcagcg cttcgcctag
    16981 tccttggata agtgttgtca caagttgaaa ggcagcgtca atcagtaaag gtaagttctc
    17041 aataagtgtt tgaacaatcg ttaaaacggc ttgaaccacc actggaataa gtgtgggaag
    17101 taagccaagt atcgtttcaa gcacttgtga gaataatgag gttactgctt ctaagagtgt
    17161 tggcaataac tgccccaatg cttgaataat ggcatccacc actgccggta aagccgacac
    17221 gatattttga atgaccggta gaatattctt gatgacgttt tgaaattgtt ccaccacatt
    17281 gccaatcaac ataccaatgt cagcgctgct gttaccaagt ccagccatca agtttgagat
    17341 tgcagacttc ataccgtcaa tcgaaccact tatcgtctca ctcgcttcct tggcggttgt
    17401 accagtgatg cccatttcgg tttgaataac gtgaatcgct tcagtcacat cagcgaagtt
    17461 cgagatatca tatttgacac cagaaatctt ctcagcctct tggagcaaac gttccatttc
    17521 ggacttcgtg cccccaaaac ctaatttcaa gttgtccagc atcccgtaat tttgcttggc
    17581 gaaaccttga taggcgttct ggatggactc aatatccgtc cccatcttgt ttgcattatc
    17641 gctcatatca gtaacagctt gatccgcaac tcctgcggct ttcttggtat caccaccgag
    17701 agatgaaatc aaggcagctg agaaacctgt gacagtttcc atgtattcat tggctgacat
    17761 tccggcagtt ttgaaagcat tgtcggcatt cttttgaacg gtggatgacg catccttgaa
    17821 aagagtatcc accccaccga ctaattgttc atagtccgcg taagcagata ccacttgttt
    17881 tcctagctcc acggcagcag cgccagcagc aagtgccact gtacccattg ctaccccgat
    17941 accttttaga acgccaccga acttttcaaa tttacttcca gacttttcag cctcgtcacc
    18001 tgtatccttg agttcgttgc caagctcatc agttttctta gcagcatcaa cttcttcagt
    18061 acccatggcg tcaattgcct tctcgttgtt agcaacttca cgctccatat tattgagttg
    18121 ggcttttgca ttgtttaatt gcactgtcca attctgagta cgcttatcgt tttcaccaaa
    18181 actgtcacta gcgttcttca aagcattttc aagggtggtg attttttctt tctgggcatc
    18241 aatcgatttg tttaagactt ggttgcgagc agacagtgag ctgatgcttt tatcgttttt
    18301 atcaaattcg gatgatacca gtttcatttc tgaacctagc accttgaatg attggttgat
    18361 ttcacgcaac gagttcttaa actccttttc accttcaacg ccaatcttta aaccaaaatt
    18421 atctgccaaa taaatcacca ccttccctta aattccactc ggtatcacat catcaatgaa
    18481 taactcaact ttcggcttgc taatcccagt aaattgctta tgacactccc ataaatccaa
    18541 aaacagtcca agcgagcagt accagaaatc atctgacgtc atacccattt gaactgtccc
    18601 atagtaatac aggcgagtaa atgtttcttc atcacttact cgcctgcttt gttttttgaa
    18661 tctgtttcac tttcgatatt gcgagctgtg cccttgaaca ttgcttcagt gattgctgat
    18721 ttatattcag ccaactgcaa cggactcgtc atcaactcca catattcagt cgtgagttca
    18781 tctttctttt ctgtgttctt caagttgtaa atcttgatgg actgattcgc caacagcgta
    18841 atcaaccaga tgatttcatc aagcgccaat tcaaagttct cggatttcaa caatttctca
    18901 ccaaggttat caagcccacc gtagtgactg gcgatttctt tcgtggcttt tgttgtcaaa
    18961 atcagttcat attcatctcc gccaaggcta atcttggcac tgcgttcttc actcattgtt
    19021 gcctcctagt ctgctgtgaa atctggttca taaacactcg tgtaccatcc attgatagtt
    19081 gtttgttcaa ctcccgtatc gttttcatca acttctgctt tccatggatg cttaccacta
    19141 gcatctaact tgttacgacg catgactgta ccctcaattg ttggtgttga gaatgtaatg
    19201 tcgtcacctt ttgttgctag gcttgtgctg ggcacggcaa acttcactcg gtacaaccac
    19261 aaatatttgt atttgccatt cgatttacga gcacggaaac caatcgcaac tggtgtgcca
    19321 ccatcttcgg ttgtcgaaat taagacaccg ttttgatcca cggttgcacc aacaagcact
    19381 gccgccacag cacgtccaat atcatcaaca ccgagcgtta actttccatt tttaaattct
    19441 ttcactactt ctgatgcacc atcatcggcg tataaagttg cttcagcaag ttccaccgaa
    19501 agctcagctg agattgcttt cgcaagcata gcgggtgtgc cgtatgtttc ttcgccagta
    19561 gttgccgact ctgtaatcgg tgcataatat aatttatcca atccaatcgt tgccattttc
    19621 agacctccat ttcataatgc ttggcgacat caatcgccac atgatagtaa tttgtatctt
    19681 tttcaaatcc tacaaataat cgttcagtaa tcgtaaactc gtgtttcaac aagttctgaa
    19741 ccagttgttt ttgcgtgttc agatagttgc ctttcacaaa caaggaaatg cgaacttcct
    19801 gtacatcaac ggttggaaag ttatccccgt aaacatcgaa cctgtcagcg agtggtgtca
    19861 gcacacaata cttgtctggc gctttatcac taaactcgcc cgtttcaaca ggaatatgaa
    19921 gtgtggcaaa aagactgttc aattcctcta aaatgttcaa atgccgttta cctcactttc
    19981 catttttgat ttcattgttt caatgcatga cttgcgactt tgtgattttg caggtttcaa
    20041 gaatggcttt gctggctgac cgtgttttcc atattcgaga atgttcgcaa tcttagcatt
    20101 tgaactgcca tcgattcgag gctcatcaaa accaaccttg atgttccagt tgccgctttt
    20161 atcttgacga gcttgtgtga taccaagggc tgattcaagt tcaccagttg aacgacttgg
    20221 tacttttgta tttgagccaa caacggcagt taagttactt cggactttgc cttccaccac
    20281 ttcagcacca ctttctaaaa ccttggggat gatttcatct gtcttgtttt ctagttttga
    20341 aacctttaac aagaaatctt ctggcatctt catcaatgct ttagccatcc gcactcacct
    20401 ccatttcagt agtcagcatt tcaaggtaca tattgcgatt tcggacattc tccaccgaca
    20461 caatctgata ccttttctga tctgaaatga caaccatctc tgtcgtaacc tctagattag
    20521 gaatataacg aatccgaaac aggcaggtcg ctgaactaaa agtcgccatg tttacccatt
    20581 tctcagtcgc attcttttcc tctttgtatg catggacagc tgcaagaact tcatcttgtt
    20641 tcactgaaaa tccagcgcta tccttttggt tcgtcacttt aacgatttgt attcgttgat
    20701 tcatctttcc aaaactcata cttgccaccg cctatcgagc ctgagtaaca gattgaccgt
    20761 ttcccagact tgcttactgg cattgacgtt atccgcaaag aagccaccag ttgaaccatc
    20821 acggctttcg taaaagttac tggctaacac aataaccgcc tgttcagtgg ttggcggcat
    20881 ttcatgttct gaatagtagc cagactcaat atgttgatag ctctcagcat aagaaagagc
    20941 ggtcagaatc aatcgttcca acagctcatc atcttcgtta tgctcaagta tgaggtttgc
    21001 cttgacctta tttagtaagt cactcatagg ctactcctta agatgaggca gatgctttca
    21061 tcttcaagat ttgcactgct tcaggtaaca caagtttacc gtccacacgt tctttagcta
    21121 agaaaccaac catgccatta cctgcaaaga gttcacgcaa ctggtcgaat gaacgcacgc
    21181 cacggtcgcc aatgttatag tagctgaaat caccaaaagc aatgactggc gcacctgcct
    21241 taacggttgg aacaaactcc gaagtataaa ctggataacc gagcagacgg tcaggttcac
    21301 ctgctaccaa agctggctgc cacatgtagg caccattgtt gtctttcaac ttacgaagca
    21361 agctgatggt ttggtcgttc atgataaact tcgcgttttt acgatatgga cgtttcaaag
    21421 catacacaag gttcaccaca tcatctgccg taactgcact ttgagtatta gttgtaatgg
    21481 caacttgtcc gccacctgtt gctgcaaaaa taccgagtgg tttaccaaca ccgtcaccat
    21541 tcaagaacgc atcttcttcc gcgtttgcta aggctttggt gaattggtcg atgatatagt
    21601 tttcaaggtt aaaggcgttg tcataaagca attcatcagt caccttgatt gccacatgaa
    21661 gtttgtgagc atcaagcaag atttgatcga acgttgcatc cccaaagctc aactcgccac
    21721 cttcttcaat ccatgaagcg gctggtttag aacctgcaat attaatcttg tgttcaccac
    21781 ttgtcgtaat tgttgtcgca agtgtacgca tgatgttttc agaagtcatg ccgtcaatca
    21841 aacgtgagtc atattcttct ggcaccaaat aaccgcctgc cgtatccaca ccttcttgaa
    21901 gaacatttga aacatgtcgg aagtttgagc gcaaggcttc aagtacacct tctttatatt
    21961 cagctgaagc acgtcctgtt ttcttaggtt ccattttatt ttcagtagtc ataggttgcg
    22021 aagttaatgg ttggctcatt ggcttttcaa gttcacgatc catcgcttcc attgactgca
    22081 tacgttcaat ttccatcgta aagttcttca cttttgtttc catttccgca taagtcttgg
    22141 catcttcttc agacatcaag ccatctttat cttgctttgc ttctacaaaa gcctttgctc
    22201 cgttccaagc ttgtgcacgt ttttccatca attcttgaat tttgttcatg ggttagttcc
    22261 tccaattttt cattaaatta agccgactca ttaacgaatc ggctgggata gtgttattaa
    22321 tttcttctcg ttcctttggt tcaggtggtt caatacgaca cttcttagca atcgctgaag
    22381 tcagtgaatt agttactttg acttccgagt acatcgaatt attaaccgga atatccgggt
    22441 caatctcacc gatgatttca tcggcaaatc ctaggtgaac agctttattt gcgtccatcc
    22501 acgtctccgc atccatcata tgtgccagtt gcacacgcga taaattcgtt ttacgttcat
    22561 aggcgttgat aatacttgct ttcacttcat caagcatggc aatggctttc tccatatcaa
    22621 ctttttcgcc aaacgcaacc gctgacggat tgtgaatcat caccatcgcc acaggactca
    22681 tgagtacctt gtcacctgcc attgcaataa cacttgcggc cgaagcggcg atgccgtcaa
    22741 tcttgactgt gactttacct ttgtagtcag caagcatgtt gtaaatactt gcggcagcga
    22801 cacaatcgcc accacccgaa ttaatccaga ctgtaacgtt acctccacct gaatttaact
    22861 catctttgaa aatctgcggt gtgacttcat catcaaacca gctctcctca gcgattggtc
    22921 cgttgaggta gagggttctc tctaggattt cctcttggtt gtcttgattc agaaccgttt
    22981 gattcttcca cttccaaaac ttcttcatct tgttcctcgc tttcttcttt gtttacgcca
    23041 taagctgccc cggcttgttc aattggcagc atattgccat tgaagtaata tttcgcacca
    23101 gcaccaccag ggattgggtt caagtcctca agttcacgca catcatcagg agataggaac
    23161 ccattttgaa taccggttgc atagccttgt tgacgtgtgg catagtcacc acgcaataag
    23221 ccatccacat taaactttat gaaatacttt tctttttcat cgcgacttaa cagtgattta
    23281 ttaagtgctt gttcccaacg attgagccat ggtgataatg agaacttcac aaaatccaaa
    23341 ctcaaatgtt caacgtttga gaaggtcgcg tggtctaagt cgccaaccat gtgaaggggc
    23401 actcggaata tccgtgcgat ttcttccagt tgaaacttac gtgtttcaag gaactgggct
    23461 tgttctggtg acatggaaat aggcgtatac ttcatgcctt cttcaagtac tgcaaccttg
    23521 gccgcatttg aactgccacc aaatgtcgca ttccaacttt cacgaagctt tcctgggtct
    23581 ttcaaagttc caggatgttc taggacacca ccaggattgg caccattggc aaaaaattta
    23641 gaaccatatt cctctgttgc aatcgcaagc ccgatagcat tcttagccat ggcaattggt
    23701 gaataaccaa ccaagccatc aaagccaagg ccaggaatgt gaagcacgtc agcttttgaa
    23761 agtctgactt gcccgacatc agcttgatat aaataataga tttccttgtt gtcatcgcga
    23821 ttaaccgtca tcttatctgg cattaaagga tacaaagcta caacttcacc tttgccattt
    23881 cggataattt gcgcataggc gttaccccaa agtagcaggt gagtcattaa ggtttcacga
    23941 aatacaaatg atgtcatttc cgaatttggt tcatcatgca gcaagaaata caacgaatgt
    24001 ttcattgctt tatctttgcc gccagcatct cggtattggt aaaagtgtaa tggcaatcct
    24061 gccacggctt ctgatagcac gcgcacacat gagtacacag ccgtcatctg catagctgtt
    24121 tgttcggtca cgcttttacc agctgatgag ccgccaaaca taaatcggta tggattcatg
    24181 atggtttgat ttgttggttt atcgcgtgat ttgaatagtg attttattat gcccattgat
    24241 tgctcctttc aaaatttgag tatcaaaaaa gcacctacga tttgtagatg ctgtgttcac
    24301 attagatata aaaccgactt cctcttgaca aaattatctc aattgcatta tttaaaaagg
    24361 tatttacttc ctctaaacat ttcgattttt cgtcaggtgt taaggatttt ctttgataag
    24421 gaaagaatga actacttggt gcaagttttt taaacgtcat ataatcttca tcatcgaaga
    24481 aatcttgtaa ataatttagt tctataaaac ttttccaatc tttgaattta gagaaatagt
    24541 ctttattatt aacgataaat ttatcagcgt tagttgatga accatatcgt ttatctctta
    24601 catcagaata aaataaatca ggaaagtcat gatacggccc tttaccttta acaccattga
    24661 gagaaggttt tgatacatat agtggcataa aatttgcaag agtatgactt aaatcataga
    24721 attttacaaa tttcactttc atctcttcgg tgatatcatc ggaatataaa atgactttca
    24781 aacgcccata atgactatct ggcattgaaa agttcttttg attatagcat ttcaataacc
    24841 gtatcgttgc accagcaatt gtgttgaaag aaataatcgt ttctccccta taattcccaa
    24901 ttccttcagc atactttgtc aaatcttcag ttagaatttt tccccaccat tttctataaa
    24961 acagtctaga aattaatgtg ttgtccgggt ctgaagatat tttatctggt gctacttgaa
    25021 tcaaatattc attcatacac atacacctcc aaagacaatt atatcaatgg attgtaataa
    25081 aagctcatat ttcacagaaa caacaatccc ctatcatcat aaacactggc accattatca
    25141 tttccacaac gaattgcacg atccagagcc ataattgtcg cgatggcacc atcaatcttc
    25201 tcagtggatt tttctttatc agctttgata ttacctgccg ggtcagttcg gacgaaaatg
    25261 ttgtccatat tccaacgaag aaccggatga ccgccatggg ctaatttctg ttcaagagtc
    25321 aacttcatca actctttagt agccgggctc atatccttga aaccttgacc gaaaggaact
    25381 acagtaaatc ccataccttc taagttttga accatttgaa ctgctcccca acggtcaaag
    25441 gcaatctccc ggatattgta tttctcaccc agttcctcaa taaatttttc aatgaaacca
    25501 tagtggacta cgtttccctc agtggtttgt atgtacccct gcttggccca caaatcataa
    25561 ttcacatggt cgcgtttaac tcgtaagtcc aaggtatctt ctggcaccca gtaatatggc
    25621 agtatcacaa acttgtcatc ctcatcttca ggtggaaaca ccaaaacgaa ggatgtaagg
    25681 tcggtcgtgc tggaaaggtc gagacctccg taacaaactc gacctctcaa actttcttca
    25741 tctgcattga atgaacaagc gtcccattta tccattggca tccaacgcac tgcttgtttc
    25801 acccattggt tcaggcgaag ctgtctgaat gagttttctt cagctggatt ttgtttagcc
    25861 gaatcacaag cagccaccac cttttcttct gcaatcgtta tcccaagtga cggattggct
    25921 ttcttccaaa cttctggatc agtccaatca tcatcttgct tgataccgta aattaacgga
    25981 tagaatgttt tatcatgctt tcgaccttca ataatgtcga gagctttttg atgctgctca
    26041 taacaaattg aattggtatc attccctgcc gttgtaatca aaaagaatag cggttgcgtt
    26101 ctagcatccc ctgccccttt cgtcatgaca tcaaacagct ttctgttagg ttgtgcgtga
    26161 agctcatcaa atacaaccgc agaactgttt aatccgtgtt tagagtatgc ttcagctgat
    26221 aatacttgat agaaactatt cgttggctga taaataattc gtttttgtga agtcaggatt
    26281 ttcactcgtt tgcttaacgc tgggttctgg cgaatcatat caacagctac gtcaaatacg
    26341 attgaagcct gcgctcgatc agcagcacag ccatatacct cagcgcgttc ttccccatca
    26401 ccgcaagtca aaagcaaagc cacggcagcc gctaactcac tcttaccatt cttcttaggt
    26461 atttcaacat aagccgtatt gaattgtctg tagccatttg ccttaatcgt tccgaataag
    26521 tcacggataa tcttttcttg ccaaggcatc agtttgaacg gtttcccgaa ccactgacct
    26581 ttcgtgtgtt tcaacaactc aataaaggcc acggcataat ctgcacgtga cttatcataa
    26641 tgggagtctt tgtccataaa ttcagttggt ttgtaattat cgattggaac tcactccctt
    26701 ctacgcaaaa agcagctgtt gtcagctgcc ttaatctctt tattccatca tatcggcaat
    26761 gaactgaatg gcttcagggt tgttcttgtt atcccgcagc aattgcacca gttcttgcca
    26821 atcgtagtat ccttcttcaa tgccatcttc attatctggc atgtaaattt gataatccat
    26881 catttatcac cctccaagat aaagcggctg tactcatctt tgtgttcttc aatgaaaatc
    26941 accaactcgt aaaagtgccg gtcatgtgca attcgctgca ctgcaatcgc atcaaacata
    27001 tttgtcagac cactttctgc aatctcaatg atttgttgct taatctttgc gttcattttg
    27061 accgtccttt ctgcctgcct cataggccgc aattaaggca tcttccaaac accaaaccgc
    27121 aatgtccaag aagtcttcac ggtcgctgtt gtgggccttc aggtcgccac gttcaggcaa
    27181 ctgaacgatg taattctttg cgatgttgta aatcttacgt ttactcactt tcatcttaaa
    27241 gcccctttct gatgtagatg gtatcttctg tttttgactc aaggtcaacc cagaccaaat
    27301 cgtggttcaa tatcatgtcc gtcacgtatt gccaagcatc attgaaggtt tcaaatcctt
    27361 ctcttctacc gttttgaaag accaattcga agtctggttt gatgatgtgc accacatctt
    27421 cgccgtatac cgcgttcagt gttgaaccgt tgtcccattg gataaggatt gaaccaatgt
    27481 cgtcaacacc ggttactgtg ccttgtgtgc ccttcgctgg ggcatgtggg tcgttcatct
    27541 tgatgagttc tacacgacta ccttcagggt aacgtttttt cataccctcg atttgttcat
    27601 gagttggaaa cataactttg tcttccgtat tcttttggta gtctatatat cactctaaag
    27661 ggattttata gcaagtgata cgttatttat tcaggatttt ctaggtcatt aaatgcgata
    27721 gtctttccat ctcgaatcac tgaaacgtct gcatcgcttc cagcctgctc gatataacgc
    27781 ttaacaatca catcacagaa cttttcatcc agttcgatcg tatggcaaat ccggtcggtc
    27841 tgttcgcaag caatcaaagt tgatccgctg ccaccaaaac tatccaacac gatgcagttg
    27901 ctgagtgacg aattggtaat cggatatgcc aacagcggaa ttggtttcat cgttgggtgg
    27961 tcaccattct tctttggttt atcaaattcc cagatggtcg attccttacg tccggtgtac
    28021 cattgatgtt tgcctttctt cttccagcca tagagcaccg gctcatgctg ccattggtaa
    28081 ggcgaacgac caagcactag actctgtttc ttccagatgc acgtacctga taaatagaac
    28141 cctgcatcca cgaatgcttt tctgaagttc aagccttccg tatccgcatg gaaaacataa
    28201 atactggcgt cgttggccat cacactttcc atacaagaaa aggcatcata caagaagttg
    28261 taaaatgcct cgtccttcag attatcattt ttaatcgttc ccgctttacc ttgatatgag
    28321 accccataag gtggatctgt caccacaaga tttgctagtt tgccgtccat gagtttctca
    28381 taagtttcag gttttgttga gtcaccacaa actaaacggt gacgaccaag caaccacaag
    28441 tcccctgttt tactgaaggc tggcttttca agttcctcat ctacatcaaa gtcatcatct
    28501 tgggcatcat cattaccgtt gagtaattta tcaagttcat cagtttcaaa accaagcaaa
    28561 tccaaatcaa actcttcacc ttgcaggtct gataactcaa ccgccaacat ttcatcatcc
    28621 caaccggcgt tcattgctag tcggttatca gcaagtatgt acgcacgctt ttgtgcctcg
    28681 gttaggtgtt ccacaaacac gcacggtacc tgcttgatac cttcttcttt agccgcaaga
    28741 atacgtccgt gacctgcaat caagttataa tccttatcaa caatcaccgg atttatgaac
    28801 ccaaactcac gcatagaagc ttgaatctgt ttcacttgat ccttactgtg ggtacgggca
    28861 tttctggcat aagggaccag cttatcaata tcaactttct caaaacgttc tgttgtgttc
    28921 attagaacaa cccccattct gctagtttct caaacccgcc aagctcattc acataatcac
    28981 gcgcaacttc cacgatttca gaataaggca cgccatcaat ctcagtgtca ccaatcgcac
    29041 agcttaactc aacgggcata ccagtttctt gagctttcaa gaatgcgtag atgttcactg
    29101 acacatcagc ttttgataag tctttcccat ggataccgcc acctgttact ccgtctgcca
    29161 tatcggaacc aagcttgcga ttagtcgcac ctgtatccac atccgggccg ccagtccaat
    29221 cacctagagg gttgactgta gcgtttggat atttttgttt taactcattt gtctttgcgt
    29281 ggctctgaca aattatcaag cgttcaccat caagaatgta ctttccatca aatggatggt
    29341 cctgatataa gtcacgcgca atttttgaga gctgtaactg ttcatcagtc aatggcatcc
    29401 cacgaaagat tccattatcg ccacaacgaa tttcagtcgc ttgattgtca gccaactctt
    29461 tatcttgggg cacaatcacc acatcttttt taattttgcc tgcgatacga ttaatcgctt
    29521 ggctgatttc attcaaattt aaagcagttg aagtttcaat aatcacatga cagatgccat
    29581 gaccaattaa aacctcaact gctatctttg gattattttc ttctgcgtat gccaagtcca
    29641 caatggctcc ggcaatacga tctgcaactt tatctggatg ttgcggatta accttctcta
    29701 tcataaattc tgttcctttc aaccattctt agaacgtaac aatcgttcca tcgcatccgt
    29761 attcggatct cctgtgtatt ccactgatgc attctctttt acaatctgtt gaatctcata
    29821 ccacgtcaca tttgcttgct tttggaagtt ctgcgacatc gccacaaatg gggaggccat
    29881 gactgccttg gtagtcgggt gttttccgag caaaccatat tgactcagag cattttcaca
    29941 ttgaacaaaa cgcgcaaagt tctgagcgta ggactcaacc attcgtgggg ctatcaacgt
    30001 ttcgcatccg cggtctttca accattggaa cgttgagtta taaatttcat cagcgcctag
    30061 cacagtgcca tccttctgtt ttgcagaaag gtaagcactt ggttcaggat aatcattgcc
    30121 ctcaagctct acaccctcac caatatcttc accaattaat aattcaacat ccggtaagtc
    30181 aaattccatc cgcgatgctt tctttccagc agcaacttta tcaacgagtg gctgtgagcg
    30241 ttcccctgaa cgaggtcgtc tgccaccgcg ttgtgttcca tcttttgcca aaatttatca
    30301 cctctttttc ttgtttgggg ctatcccccg tttgaactga acttttttcg cgtaagggcc
    30361 tagcgcgctg tacagtttag aagggtttag ggataattac cgcccctccc ccccttgtta
    30421 tttttcacat gattgtttta tcttttgtgt ttcgtcgagt catgtgaatt ttcgagtgac
    30481 atgatttgca gacactcatc aaattatttt catcatgcgt accaccatca cttaattcct
    30541 tgatgtgatg cacaatctca accgaagtaa agcgtccttc cttcagacac aactcacata
    30601 ggggatgtgc tgccacgtac cttgcccgta ttttcttcca cgctctgccg taacgtttgt
    30661 tgattgccgg atcacgctcg tatttctcgt aacgtttcct cgcaagtgtt ttatgttcat
    30721 cacaataagt atcatgcgta cggttcggac aacctgggaa cttacagggg ttagctggtt
    30781 tcattggcaa gtggggtcac atcctttcgg gcaaaagaaa agcccttgaa ggatttttcc
    30841 cacaaaggct tcgagtattt gttcgatact tctattgctg gtattgcttg agtatcgttt
    30901 gtcttttttg acatcttaat gatatcacta aaggctactg acattcactg acacatttca
    30961 aatgactatg ggttcatctg gtagcttcaa gttctcaaga gctttgctat gccaacgaaa
    31021 gactgtccgc acgtcagcgt gaagatcttc agcaatcttg tcccagctct cttccaacag
    31081 gtatcgattg attaacacaa tcttttcgtc cacgtttgtg agttcatcaa tcacgccacg
    31141 catttgtcgt ttgaggtcta caaacttatc aatctctgca tcaatttcag cttccaattg
    31201 gtaacacttc tcaaggttac gaacaaatgg tgcctcagta ttccgtgtgc cacttgaaat
    31261 cttttcttcg aatgttgtgg aagaaactgt caccgccagt tgatagagct ccgccttctc
    31321 tttaatccgg tcgtcaattc gtttattcaa acggtacgct tgtttcaagt attcttttgg
    31381 tgtcacttgc ttccctccaa tctagctttc actgcatcaa tcaaacatga ttgtgtttta
    31441 tcttttcgtt tcaaagccaa catcacatct tcatcaatcg tatctttcgc aataatgtga
    31501 tgaatgacca cagtatcact ttgcccttgt cgccacagcc ttgcattggt ttgttggtaa
    31561 agttccaaac tccacgtaag accaaaccat acaagtgttg aaccaccagc ttgtaaattc
    31621 aatccatgtc ctgcactagc cggatgaatg acagcgatgg ggatgctacc ttcgttccaa
    31681 tcaataatgt cctgagacgt tttgatctgt cgaacgttga atcgttcctt gatacgctcc
    31741 agatcatgct ggaaccaata ggcaatcaga atcggtttgc catttgtacc ttcaatcaaa
    31801 tcttccaaag catctaactt ctggtcatgg atgtgatgac tcacattaaa ctcatcatag
    31861 attgctccat tcgccatttg tagcagttta ttcgaaagca ccgctgtatt ggctgcatca
    31921 atggtggctt ctttcaattg tagcaccaag tctgccttaa tttcatcgta cttttgcttt
    31981 tctttgagtc ccatatcaac tggcacttca ttcatcacaa gctctggcat atccaagaaa
    32041 tcgactgact tcattgaaat cgtgatatct gaaatcttgt cgtagatttc ttgttctgca
    32101 tcttcccttg gtttccaaga ataaatctgc atgccatttt gcttgtcagg tagaaagtat
    32161 ttatctcgat aacgtgaaat ataatagccc aatctctcgc ccatatccag aaccttgaac
    32221 tccgcaaaca aatccatcaa accattactt gaaggtgttc cggtcaatcc aacaattcgg
    32281 tcaaccttgt gtctgacttt cataaagcta gtgaaccgtt tagcttttaa gtttttgaag
    32341 gacgacagtt cgtcaatcac caccatatca aaatcaaacg taaaacccga tttgttaatc
    32401 agccagtcca agttctcacg attgatcaga tagatgttgg cattattttg taatgctgcc
    32461 attcgctgac ttggtgttcc aagaaccacg gaatacgata aatctttcaa atgatcccac
    32521 ttttgaattt cagttggcca tgttgattgc gtcacgcgca acggtgcaat aattaagaca
    32581 cgttgtattg taaagtcatc atgcatcaag tctttaatag ctgtcagcgt tgtcaccgtt
    32641 ttacccaagc ccatgtccaa tagaagggcg gcagttttat gatctttgat aaaatcaatc
    32701 gcatatttct ggtagttatg tggtacgaac ttcatcagac atcacctcct attctttcta
    32761 gtaactcagg tatctgatca atctcatcaa gcacgaaaca ctgaaaccct aaatctgtca
    32821 gttgtttcat acgaaaaagc tgtaaagacc gtggcttctt ccctggcgcc ttcacttcga
    32881 caaatccaag tttcccattt ggcataagta ccaatcggtc aggcacacca gcaagccccg
    32941 gtgatgtaaa cttcgggcaa ataccaccta cccttttgac ggctttcact agtgcttgct
    33001 caacctgctt ttctctcata gccacaccgt caaatactcg aaatacgcat cacgcaagat
    33061 ttccaagtat tccttttctg cgcagtgaat catcagatgt gcgacttgct tatcgaatga
    33121 tttcttccaa ggatacgtga catccgacat ggcaaactcg taaaactcat caaatcgtaa
    33181 atcacgttta ccgattccaa tttgtaacca ctgtttaaat ttcatttaga tattctccaa
    33241 tctgcaagta tggtgtaacc cgtgaatacg caatacataa ctttatatag gtggtaattt
    33301 tttatccctt aagaaaagtt ctatataaga cctacacagg ttacactttc ctttaaaatc
    33361 aactattcca agaactcgga tttgagttgc aaaccaacaa tcttgtttcc tttattagtt
    33421 cgccggcgct taaatccgcc cgcctccaga gccgtgtaaa aatcggctga actacgaatg
    33481 tactcaccag ttcgcgtaca aaacgcacga tactctgcat acaactcacc agatttttct
    33541 tcaaagtcaa cagcagtctc gcagcactcg tctaagaaat ggccaagcca atcattcgcc
    33601 gtcttatatt cagaaattgc attagcgaca acttgtggaa ccgttaaatg aaagtctgcg
    33661 gcgatgactt ttttcgcacc gtcaaggatc cattgcaaaa tggctccgcc ggcattttca
    33721 aaaaggtagt ccgcataatt cttgatgtcc ttattgccgt caatcgtagc aaggaatgga
    33781 atcacaatta aacgacgcca ggttccttta tcaatcgctc caacttttgg gaggtggttg
    33841 gtatagagca ccaaagtatg cgttgggaca tacttgaatg ggtctttgta tttcttctca
    33901 gccgcaattt catccgtcga gcagagctgc ttaatgttag atgtgttgag tcgcatcccc
    33961 tcttcaagct cggctgcgat gagtaggcgt ttccccttcg cttccgcaag ttcaggtttc
    34021 acattgcgtc ggatttgact tgtaagaata tcagctgaga tgctgccaga gtaattaccg
    34081 agcacacggc tgatgacatt ccagaacgtg gacttcccgt tacgcccttc accatacgag
    34141 atgataagcg cttcaacgta aactttacca atagctgcaa gtcctacaat catctggaca
    34201 taatcaatca actcttggtc acctacgaaa atggtgttca gagcgtccaa ccaaatctgc
    34261 tgattggcgt cactcggggc acattccgtt tgcttcgtga tgtaatcttc tgagttgtgg
    34321 tcttgtgatt tgcctgttcg caaatcaaag gttgctgttg gtgtgttcag caagaactcg
    34381 ttcacatcta gtaaccgttg ctcgatttca agcatgggct ttgcctcggt taatgctgac
    34441 gagagatact tgctgtcgcg gcgtttgaca gcaaacttct tatactcttg tgcgtgttgg
    34501 tacaaatcaa aactatgacg ttgcgctgtg ttgaacatgg tgactgcctt tttcggacca
    34561 acactagcaa gtacatcgaa cgcgccatta ctaaccattt ccttggtacg cttctcaatt
    34621 tcggtttcag attcttccag ttgacgttct gtcaatgcgt ggatcacacc ttgcgctttt
    34681 ggtttcgact cttcccaata gctgccgttg tagacaaggt aaccagttga cggtgagtaa
    34741 tgaagcttac attcatactc gcgagctaag accgttgctt gacctaagtc cgagtagtcg
    34801 gttggctcaa gtgataaatc agagttgtac tgctctggag gaatgtagcc ttcttgttgt
    34861 ttcactttct tcccaaagtt gaccgcgcta ttccagatag ctttaagctc ctcctccggc
    34921 agtggcggat tacatttttg ggattgttcg agatacttgt gatacgtatc ttccgttgca
    34981 ccgtagcgtt tgatgagctt tcctgcgatg tgactcatgc ttgaattacg gctgccttcg
    35041 ctaatctctg actgcgcatt atcccaatca gcaaacagat catcttcgag aaaatcagtg
    35101 atcaacttac taccttcata gatttcaact tcagagtttg gtgttccgaa aagcaaacgt
    35161 gccgcatcca tcgcattgtt gtcaaagtat gggaactcac tttgaatctg cttcttgagt
    35221 tccacatatt catctttatc agttgtggca ggaatcggga aatatacgtg aaaacgtgga
    35281 cgtgctgatt tgccaccttt gtctttcatg ttgttacgtg aatagctgac cgcaaacgtc
    35341 acatcaggaa actgcatcgc cacttcgaaa ggggctaccc aatcatctgg attgtctgag
    35401 tggtcattgt cgcaatcaag tggaatcaca tcagactgga tgaagttgtc acctttgcga
    35461 tagttgcctt tgtactcagc catcacatga tccagcttga ccgtggcaac aagtgtttct
    35521 ttatctgtca ccacttgctt ggttgggaac aatccattct tggaattacc cacctgattg
    35581 gatgtgaata acgtcatctt aagcatttga gacctccttc atctcatcat cgaagtacct
    35641 gatatgtttc ttccaatact ccgcttgttc aatttcaact gccatgcctt gggagatggt
    35701 gtcacccacc acccagactt cctcacattt gcccatgaga acaagtgaca tgtgcattgc
    35761 cacatcacgc tccgttgcgt cgtccatgaa ttgtggatac atcagatgtg ccgcaactgg
    35821 catgtagcca cggtcaagag caatacggca gtaacgctta gtgtttttat ggttgacgac
    35881 aacatttcct gagaacggcg agcagacata gactgatgtt ctgtaatctt tacgtctagg
    35941 cattattatc acctcgctca atcactggta agatgttctt cttcgatttc agcaagtcgt
    36001 acaagaacaa acgacctttc tgcgtccagt aggtatgcat cacgctgcgc tctgcatcta
    36061 tcgcatgagt ttttgattgt gtgtagcctt gattggcata cttctgataa agcaaccaca
    36121 tcttgccttg cttgtactga attcccagtt catgaagcaa cttgttcatt gcctgtggtg
    36181 acataccgta gtccttcgag attttagaaa tcgacatcac agaattgttt tgaagaatca
    36241 aatcgtagta gctaactttt ggtttcatct ccaaaatcat ttgttgttgc atcgccacca
    36301 tttctttttt ctcttgtagc tgcttcaaca ctgaaatggc ccatgctgga tcttcgattg
    36361 actttgtgat gaaatcttct gtcgcgtaca caccgtgttg gcggatagac ggcaagacct
    36421 catttgtcac ccaacgcttg aatttcttcg cgttcggcat cttgctagaa atgatgagtg
    36481 agtacaatcc actttcgttg ataaggatag ccattgtccc attaacgctg aacgattcgt
    36541 tcaccgtctt atcttcctca tcaacatgat ctcgtaatgc tttttgtgga tttgtgtagc
    36601 caagaattga tgctacatct ttaccaacaa agtgagggac atcgccaatc atcaccgtgc
    36661 gtacagagcc aaactccgca ttttcaaaaa ctttcaattc gttcatcttg tgaacctcct
    36721 gtaatagaat tttcaggaga ggcattttgc cccttctaac ttacaggcga agaaaatggt
    36781 cgaaagtaaa ctctgactcg aatttatttt tgattttttt gccttcgtct ataagcgaag
    36841 aaagaaccca attcgaacct tatttttgat aaaaaaattt gaaaaaaata tggagccatt
    36901 tctgactcca cattgactaa tccttttgat agaattcaca ctcgtatcca tcagcgttca
    36961 gtagcaagcc ttcagcccac ttaggcgttt gactcatcaa ctgacagatg tcttcaacct
    37021 tcgattccat tggcacttcg ataatcactt cgtcgtggac gtgcccgaca atgcgatact
    37081 tacgaagatt ctccattgcg aaacagagaa tgtctctagc tattgcctgc acgatgtttt
    37141 cgacaaactt gggaccatag ctttcaagac gttcccattt cttggtagca ccgacacctt
    37201 cataggtaac agcttcaccg ccgaatttgt tctcaccaat ctttggtttt acataaacta
    37261 atttacgtcc tgacaagagt tggatgaaca gaaaacccga cttgtattca aacttgatgc
    37321 catgcgtcgc tgtgctccgt cgttctttca cacaagtctt tactgcctta tcaacagccc
    37381 accaaagctc cgttatgtta atgtttgagt ctcgccaaga attaacaagc ggctgtaatt
    37441 catcctcagt tagtcccatt tcaagagcat ccattgcttt caatgcaccc accgatcctc
    37501 cgtaccctag agcaagctca gcaattttcc ctttttgacg tagatgccca ttcacgccat
    37561 gcttttcaac tggcacaccg aacatctgac tggcagacgc acagtaaata tcgccaccat
    37621 ttttgaatac ttcttgacgc cacttctcac ccgacagcca agcaatcaca cgtgcttcaa
    37681 tcgctgagaa gtcagatacg ataaatttca tcccttcacg tggtacaaac gctgtccgga
    37741 ggagttctga gagtacgttc gggacattgc cgtagagaat gttaagcatt tcaaaattgc
    37801 tttgttttac taaactacgc gcttcagata aatctgacat atggttctga ggcaagtttt
    37861 gaagctggat gccacgtcct gcaaagcgtc cggtacggtt ggctccatag aattggaata
    37921 aaccacgaga acgattatca gaacacgcaa agttttgcat ggcttgatac tttttcacgg
    37981 atgacttggc gagttgctga cgtaagacca ggacttgctt taaattttca tcaataggtt
    38041 gttcgagtag ttcggccact gcctttttac cgagtgactc ggtttcaagt ccgtggtcta
    38101 gtaaccatcc ccgcatttga agcacgctgt ttggattttc tagtccagtt aattcttgca
    38161 attgttgatt caatccagct tttgataatt catccaactg aacggcagct tccacaaagt
    38221 ccttatcaat ctcaattcct cggtcgttga tttcctgatc cagccagtat tctttccaaa
    38281 cagaatcaag cactggaaaa ttggaaagtt ttccttggat agctatttca acttccacat
    38341 cacgactatt gtatgtctta aatgctgacc atttagttgg cgcatggtgt ggtaagttcc
    38401 tcgtgcgtcc accattaact ttggtcggat tgcatggcac gcaaaagtat cggatgaggt
    38461 cttttccttc tgtgagcttt tgtttatcca aaccaagaac agcaccaact cctacaagtg
    38521 acaacggcaa acctaatgtc gcaccccaca ccattgtgca ttgccaccct cgtggattca
    38581 aaaagccatc caaccccaga aagcgtgaca aacacacacg ttcaaaactc gcattgaagg
    38641 cgtgtttaat cactgtttca tctgatagcg aagttaatat ttcagccggg atagtttcgt
    38701 ccatcttcag atcaaccact tcgacgggac caccatctac actgtatgca aatagcagta
    38761 tttcaaaatc gggggactct gaatatttgt agacaccaca tttcgataaa tcgacactac
    38821 tatacgtttc caaatccaat gacaaatgtt tcattttctt cctccaatca aacaggcgac
    38881 agcacttctg ccatggccgt ttttgtttta atgattaagc caagaaatca tcatccgcca
    38941 aggtttcaaa ctcatcgctt gcagatactt gtgagccgag tggttcgccg tcacgtacaa
    39001 actggatatt cccaagccct gccgctaccc cgcgatttcc atttgcgtta aaggcataga
    39061 aattaatcga cacacgagcg tagcaaccac tatacacttg tgaacgatcc aagattggtt
    39121 gaacgtgctg atccacgatt tgtggtgcag tggttgagtt cgcattaatg aagtaatggc
    39181 cttggtatgc ttcatcgtcg cgttcgatat ccccatcacg taatggcagc ttgatttctg
    39241 ctttgtttgg tttcttccca ccgaatttcg caagtccctc ttcgattgct gcatcgattg
    39301 ctttttcaat ggtatcaatc gttctcttat ctgattttgg aatcaagact gctgttgaat
    39361 atttctctgc gcccccgttg atggatgctg gttcccaacc gtggaagtat gagagacgtg
    39421 tgtttgtacc agtgattact ttgcttgttt tagacatatt attgttcctc cgttaatggt
    39481 ttaaattctt cgtttaagtt gtttactgtg accgcttgtc gtttatctga accatcgact
    39541 agcgtcagct tgccttttgg tttgatgatg tattcgccca agatttcatt gaatttctct
    39601 ttaccgagtg ccttcgtcat tgctgtgaca ccaagtaact ttttatcata tgggtcgtat
    39661 ccagccttga tgaccgccgc agcgacatca ttgtcattag aatacttacg agtagagcga
    39721 ccctcgacta atttgtgatg agtccattgt ttgccaccga gtgcaagttt caaagcacag
    39781 tccttgatat cgttactcca agaaaccagg tcatccactt taccgatgat ttcttcgatt
    39841 tcatcatcac taagaagctc tggtggtttg aactcatatt gcgccagttt caagttatgg
    39901 tctgctcgtg cacgacagct attcttcgcc ggacagaatc cacaccattt cccgcattgg
    39961 tactcaccct cacctttgaa agctaaatct gcggcaggcg acaacacatc ttttgcccac
    40021 tgaagtaatt cttctttcag cagtgtgtac gtactcacat tctcacgcct tggctggaaa
    40081 atggtcatct tgacttcttg aatgtcataa ataacatcga aaagctccaa cgctcccaag
    40141 gcataacaca tcatctgtgg gttctgatac gcatctacca aaattcctag accgtatttc
    40201 atgtcaataa cagatagggt tccatccgca atgacgagac agtcgccggt cccaaagccg
    40261 tctgggacgt accttgagaa gtcgagccgc tgttctacta ttacaataga gtcaactgtt
    40321 gtctgtttcg ccttagcgac ttcttccatg acgaaggtcg cataactttc agcacactgc
    40381 tccatctcct cgtcgtagaa ttcaagcgta gatgttgggt ctgtgacgtc attaccaatg
    40441 aacttattca gtttgtactc acataaggta tgagcatcag tgccttgttg ggcatacggg
    40501 ctcgacttgt tttcaaagtg ctctgacaag cgaacacttg gcggacagtg aatccaccga
    40561 ttacttgaag atgcggatag gactgcgtgt tgtgttggca tctacaatcc ctccgcatct
    40621 gcaaccaaag ccgcgtagtt ttctgtcggc acatcactca agcgactggc accatatttc
    40681 tcaatcagtg ctttcacttc agttgactta ccttcttgag atttacgtgc caacaaacca
    40741 cggacttctt caagcgtcgg ttgtttgact tctgattttg tttccattgt cggttctggt
    40801 gtttcaactt gtggttcagg ttcattggtt ttcatcgcca cagccaactc ttgtaagcta
    40861 tcagctaacg cttggacgtc ctcaacgacc tgaagaaata gttttgtttt actcacgtca
    40921 ttacgctccc ttcttgttaa cttcacggat ttcgactgat tcgattgtgt cccctggtgt
    40981 gagcacgaga attttgtcca tgttgccctt accgaacagc caccgtgcaa accagcgagg
    41041 caaatgacgc acaccacctt gtaggacagt ttctttgtta cccgtgtcat cagtcacgtt
    41101 tactcggatt ttgtgtttta ggttcatgat ggtcctcctt catctttatt tgtttaggat
    41161 ctactccctt acacaataca ggcgaaggaa aacggggaaa gtaaactccc ccgtaacttt
    41221 tttctaaaat aattctttga tcttgtcttt gatgattcca aaggtgcgat tgattgactg
    41281 ttttttcttg tcaagcttgt ccgccacttc agtctgagac atgttttcag tgacgagcat
    41341 gtgatagatt tcttggtggt acggtaataa ttgcgtcacg acttcacgca aacgatcgat
    41401 acgaggatct tcttcattat cttggtaagc atcgtaaacc aatgattgga tggggttgtc
    41461 cccctcatcg cattcatcat caaattcatt gagcgacaaa ttgtagttac gtttgaagta
    41521 atcgttggct tcaccatcca cgtattcgtc cattggataa tagccgtgct cattattaaa
    41581 gtcttcacgg aacttctctt tccaagcctt gatcttttct ttttcttctt ctgtgcgttc
    41641 aggacgtgcg tttttataaa agttgtaaat cgcacggtcg tcagcgcgat gcagctcctg
    41701 aatgttaagc tcagtgacgc cgtcttcgcc tgggcgaatc tcaaccactt ctttttggat
    41761 acgtccattg gcatcagtac gctcaaacac gtacttgtaa gtgctgcggt cgttttggtg
    41821 tgttttcata aatctactca ttagatccct ctttcttgcc tgaaagagct tttgagcaag
    41881 gaaaacaaac agggaccgtg ccaatacgta caccgtccct catcacgaaa aaagagcgca
    41941 ataaagtaag gcatacaata tctgaaaaat tttctggata accagttctt ttcagtattt
    42001 gtaaccttgc cctattgcaa ctcaggcatt gttatatttt attagtagga ctattcgctt
    42061 ttaagataaa ttcgttcact ttcttgaaac ataatgtagt gtttatgaac cataaatgat
    42121 aaaattaaat agttaaacta ctatgtttta attatagaaa aaggcctccc ttgttttcgt
    42181 cagtcatcgg cggcaaacgg cggtcaacgg cggacgtaac aaaggaggtt gtattttgga
    42241 aataagtgaa attactcaaa cgctttatcc gtttatggga agcgagtacg tgaagccaca
    42301 ggatttttta attgctttac tggatagtgt ttcaaatgat gacgaggcca ccattttcgg
    42361 tttttctgat gataacctac gaagatttta caatggtact cgaccattaa cacaagagta
    42421 tgcagttcgg gttgctggaa aattcaacat cgacaaatgc acgactttta tttatgaacg
    42481 tcttatagat gatgctccaa ccgtccttaa gcaacaattg attgataaag gtgtggtaat
    42541 ccccaaagat acagacgttt ctgaaatagt ggcccatctt ttacttgatg aaatcacctc
    42601 aattgcttct caaacaaaag taaaaacaaa aaaggccaag ccgaagctta gcgaattaga
    42661 gctcgcaagt ttaagaatca acactaatgg tgatttctct tataatgatg aagtgaaaaa
    42721 attctttgac gatttgccag ttccccttga tattagagct gaggagatga cgtatgtcat
    42781 agctcttttt tcagcatatg cagatgccga tggatacgaa gtatacgagc atacaaacga
    42841 cctgccaaat tcgcggctaa aggatttcaa gagacagcga agaaattact ataaagctga
    42901 aagcatacgt cgtggggtcc gtgataactt tacacaagag gaaggaactg agcactttga
    42961 agctttaaaa gaagatatgt acgacggcat cgaggaagtc tatgaagaag actacgatga
    43021 tggcgttgag agactcaaag ccgttctaca gcagtcgtct gttataacat taaacggatc
    43081 aatcttatcg tttataccgg gattgctgca aaattctgct aaaaaaggca tttgccacat
    43141 gctggttaat gacggaaaga tcaaatgggt gactgaagat gaatagtttg tttaatactc
    43201 catttgaaat ttcggttcga gtaatgttac tgctctctga atttaaagag cctgtcagcg
    43261 ctgatagaat tttaatcaca gacttcatca ccacgtatgg tcgcgacttt gaaatttccg
    43321 attacaactt aaatggtgat aacagctata gattcagtga atttattgcg cgacgtgaat
    43381 tagttaaaga tgcagtcaaa aatcttgtac tgcaacatgt tatccaacca tcgcaaagca
    43441 aagaaggttt taagtatgct ttatcagaag atggcctaaa gttgatatca caattcgtat
    43501 cggtatatgc agaaaaatac ttacaaatag ctgacgaatc tgttaaatac attcaagata
    43561 aaagtgacgt tgagcttctg cgacaaataa accaacgcgc aaaggaattt aaaggagagc
    43621 aatgataatg caaaattatt atattgaacg tgttaccata aaaggcaaag atcgtgaaga
    43681 tgcaactgtc aagttcacca atggactaaa cataatttct ggcccttcaa ataccggaaa
    43741 aactagcatt gttacggcaa tcgattttct ctatggcgcg aaaaaagacg ctgctccctt
    43801 ttcaaccgat gcaaccggat acagtaaagt tgaactgatt attcaatcga ctaatggcaa
    43861 atattcatta actagagaat ttgatagcaa ctccattttg gtatccaaaa taacgagttc
    43921 aggtgaatat gtagattatg aaaaatataa tgcacgatct gggaaaaatc caattagcca
    43981 tttttggtta caacttttag gcatcgatga tgattatcag attatcaaga atgaggattt
    44041 caaacgtcaa aaattaactt ggcggacatt agcgcctagc acacttattc cacaagatag
    44101 agtcaatacc agcgcctctg ttctttttcc tggggaaaca acccaacgca cagctttttt
    44161 atcatcgttg ttacttctgc tatacggttc agattttgcg aactcggaag aaaacgaaac
    44221 aaaagaacat aaaaaaatta gaaggaactc ggtgattaac tacatcaatt caagcctttc
    44281 tgaattatat gaaaactacg aagtggctaa atcagaatta cctgaggctc cctctgatga
    44341 ttttcaacaa attgtcgatg gaatgttcgc tgaacttgaa acaattgaaa agcaaatcaa
    44401 tgatgcgtta gccagcagca aaatcttgat aaaagagatt tatcaaaagg aacagtatct
    44461 tcatgaaact caacttcttt tgaataggta caaacagtta cattatcaat atcaatcgga
    44521 tattgaacga ctaacactaa tcgtagatgg tgagcgatta ttaaatgatc ataatgaaca
    44581 tgatacttca catagctgtc cattctgtaa cggcgaacta agtaaacctg tccaaacaac
    44641 ctacatcgat actgctaggt cagaattaca ttcattgctc tctcaattat cagatttgac
    44701 ggaagaatct catgataccg aagttgaact gcaagagtta atggaacagc ttgatgagct
    44761 taaaacaaga aagaaatcgc ttgatgcagc aatcaacgat gaactaaaac cgaaagctga
    44821 gacgttaaag gatcgaatca ataaaatcga aacttacagg gatattaaac aacagctaat
    44881 ctcgatggaa aaaatgcaaa ataagcttaa aagtgatctt gaaaaaatcg agaatgagcc
    44941 agaagacgaa cgtatccttt ataaacctaa agagaaattt gatgcagaat tctacaaaga
    45001 aatgtctgag ttactggact ccatgttacg taatatgcat tataaaccta atgaatttcg
    45061 taacgcattt ttcgccaagg atattttcga catcaaaatc aatggaaatc taaaatggac
    45121 cgaaaacgga catggatata cagcgtttat caattcagta gtggtaatgg catatcgaca
    45181 tctcttgtat aatcacgcta agtacacacc ttctgtgttc attatcgatt cccctcttct
    45241 tggattagat gaacgcaaaa aacaagatgg cgatgacaat atgaaagaag gacttttcaa
    45301 atactttatg gatcatcaaa acgaaggaca acttatcatc gttgagaata caacgaactt
    45361 gccagcacta aactatgatg ataaacaagt aaatcagatt gtctttactg aagataaaaa
    45421 caatggacgc tatggatatc tatacgatat ttataactag aacaggaatg attacatgaa
    45481 gtttagctac aatccgcttt ggaaactcct aatcgacaga ggactgaata aagcacaact
    45541 cagaaaaatg actgatatca gttcagcaac actctcaaaa ctaaataaag gcgaaaatgt
    45601 aacaacagat atcttacttc gcatctgtga agcacttgat tgtgatttat cggatattgt
    45661 tgaaatcgtt cgcgattaaa tatcaaaaat gcactgtatt cgattcatga gtacagtgca
    45721 ttttcatatt tatttttgta atccagcgat tacttcattg attttcgatt caatatcatt
    45781 caatggaacc atataccatt cagtagcttt tttgaagttt ccatcctgaa agacaacttg
    45841 tttgtcctgg aatacatgat ggagagtgcg ctcaagtagc tgagcgttga cattttgtac
    45901 ttcagcagaa agcatgacct tcactggtgc atttaagtag gtcacgtcgt tttccgcatt
    45961 ggcaattcta ctttcaacat catttcgagt gaaaccaatc ttatacaggt tattccactt
    46021 gctaatatcc ggattcgtgc ttaatgactt cagcacgtag atataacctg tcgtaaactc
    46081 ttctccaaag ctttcagcca gtgtttcctc atctaccggc tctgtcacca tccttccatg
    46141 acgttcagtc gagtatagag acgaagctaa agattgtaac aacagattct tattctctgt
    46201 cccattctca taaacaacat gcattctggc atttgttttc ccattatcag cgataaaggt
    46261 atccttcgaa atggaaacga tatacatcat tacgccgttt tggatgtaga atctattttc
    46321 atgaatatca tactgctcaa acgggataat ttttcgtctg ccagatgcga tatctgcatg
    46381 cacttgatcg aacaagctct tatacttttc aaaatgactt gcatgttttc tgcggccaat
    46441 tttatctgca gcgttcacag ttctacgata gcgggaaaca tccaacaagt ctgaaaaatc
    46501 atcaatcata tcatctgagc ctaagatatc atctaaattc aactcgtcat cgatttcaac
    46561 tgaatcagcg agaaactctc catccggttc gaattctttc tccaataaac caagagtgtc
    46621 atacttctta accttctcag acctttcggg ttttcctcga aatccgctaa gacgagccca
    46681 caatgcgcgt tcactagacc attcaggagt tttttctggc tctcgtccgt tttcattcac
    46741 aaagtctata atctcctgaa atttagcaac ttctgggtct tgttttacaa ctttcttttt
    46801 ttcaacttgc ttggttaaat catcaaaagc gtcatcttca aagatgctat ctaaactatt
    46861 cgccatcttc tttcgctgcc tctctttcac gttttatctc agcaagtcgt agcaaagcaa
    46921 aggccaagta tttttcacca tcatctttac tatttcgatc aggatttcga ccattttctg
    46981 cacggaactt cttagcagct tggtaggccg caaccaattg ttcgtcagta aaatcatact
    47041 tatttgatga caaataatcc tgaataaacc gaagagtcgg tgcatcaata tcacgactaa
    47101 tcacttcata cgcacgttgg aacggattca cttgatcgat taaatcaata ttcaattcat
    47161 caatgttaat gaaacgatcg gccatcttaa taatttcgcg gctaccatct tctgaagttg
    47221 ttgtccgtcc agccgcaata tttatatcgg caactgtatg ttgacgaacg gtttctaact
    47281 cttcgttcgt caaatcagga taacgatcaa tgatgacacg tggaatcaac gtcttattaa
    47341 tcatcttcgc atcagcacct gaaacaatcg cattttgaat atcttggctc tgtaaaatgc
    47401 tagatttcaa atcttccata tcattttcga tgattttctt tgtacgttcc gtcgtatcag
    47461 caggtttcag tcctctgatt gaaagatcac ctggtttgtc tggcttacca tcttttggtt
    47521 taaacttgta aactggcgcc aacacttgtt ccataagtag agatgcagaa attgccttca
    47581 taatactgtt aaccgcgaat aatacttcgt catcctgtgc atctggctgc gcaataagat
    47641 ttgtaaactg tgcatgagtc ttattggggc tgtcacgagt tacgcgacca atgatttgaa
    47701 tcacttcagt taatgagtta cgataaccga ttgtcagtgc atattccgca aatggccaat
    47761 cgaagccttc ttttgccatt ccaagtgcaa tgatgatatc aagcttatca agatctttaa
    47821 tgtcatccgt aatacttgct agatatgctg agactttttc acgtccttct tcattcacaa
    47881 ggtcggccac taatagtttt ttaccatctg gacgttcgat ttcataaata ccatcctcgt
    47941 tctgtgtgat attaccaccg tccgtcaata aatcaagtat ttcatcaact tccgcgtatt
    48001 tatctttagt agactcacca gaattaacat taggaatatg gataattgat tttttgttga
    48061 catcaattac tttatccaaa gcatctgtat attttccacg atagaaacta taccccattg
    48121 caaaggattt cagatattta tatccttcca gttgctcata ataagtgtaa gtgaccttat
    48181 caaatttatc ttcgtcggct ggttccagaa tctgaacaga gtcgccacgg aaatatgaac
    48241 cagtcattgc taagatatgc gcattagagt ttgacatgat gctacgtaag gcattaccta
    48301 acacagattt atcatcacgg ctcacatgat ggaactcatc aatcgctaat agcgtattgt
    48361 caaataatga atcgtccaac tcttcaaaag caaatcgcaa cgtagcatgt gtggcaatca
    48421 ggatattatc acttgggtca gtcgagttca tgaatttaac aaagtgttga acctttgatt
    48481 tttcaccgcc cccaatagtt aagttgtcac ttggctttac ttcccaatcc caataaaaac
    48541 caaacttcga taattcagta tctttaaatg atttaccaat tgagcgttca ggaactgcaa
    48601 taaccacttt tttaattcct tgattatgga gtttatccaa gccaacaaac atcaatgcac
    48661 gtgattttcc tgaagctggt ggtgatttta ccaacaaata ctgtgacgca cgcttttcat
    48721 aaacacgttc ttgcatttca cgcataccga ggtcatttgt tgtttttgat tgatttttat
    48781 caaaattaat ttcaaataaa ttactagtca ttgattgtca tctcctgata gagttttagt
    48841 aatgttccaa gtcgatcttc gtcagattca aacgggcgtt gctgataagc tctatcaaca
    48901 atcccatcca gttcctggtg tttcttacgt aatgattctg gcatactgtc tttattatac
    48961 aaatcggcta acgtgccgcc ttcgtattct cgtaaatcaa gaatttctgt catcacgcga
    49021 gtcatttcat ttttacgctg ggcggataaa tcctgaatcg gaaaagtatt atagactaac
    49081 ccggctgaat aacggtaccg agtttctaat ttcccaccga cagagcgcag ccacaccata
    49141 tgcattcggg attctaacag accaagcagc cacattggag cgttatagat tgccatagcc
    49201 gaatctgaaa ttatagtatc gctcccaact atacccatag gaacataatc tcttttttct
    49261 gatgacacac gaggaattag aatactattc tcactggttt catcataacg aacttcacca
    49321 aatgaccatg gcttctcagc tagtgctcga gtttcttttc tcttgctttt ttccctataa
    49381 ctttttacat tttttattgt ttctttaata agagaatttt gtttgtatct agttgcatct
    49441 tcctcggtca accataaagt atatcttaag tctccattta ttaatcctgt tgatcctaaa
    49501 tatttcttaa ataaatgttt aatatctgga tatttttgaa tcaaattcca gtattctgcc
    49561 actgaaaaaa ctagattacc accatcattt gccatactgc caaatttcaa agagggtaaa
    49621 tttgaaatac tatttcttga ttcgttgaca aatacatttt caccattagc taaatatgca
    49681 ttaatatttg aaacactcat tactgaccca ttctctaaat ataacttagg atgaatattc
    49741 acattatttg tcataccaat aatgacaaca gtcacaccgg cattattctt tgcattgttg
    49801 ttccacttaa agctctgata tgcaaatgaa attcttacct catcttttaa aaggtacggc
    49861 caaaggatac taacttgttc accttgacaa attgaattag tagtaacaaa tgcgaacgag
    49921 ccatgagatg ttgcaatata ttgtttagct ttccaaaacc acccaaaaat ataatcaagc
    49981 aatttatatt ttcgaatatc gccaacaagt ttttccatat cttcacgttg ttcagcattt
    50041 tgaagacttt tgcccagata cggtggattt cccattatat atatttcatg attttctttg
    50101 agtcgcaaca cctcattcca atcaattcga agagaattgg cacacactat attcccagca
    50161 tcttttaacg gtaacaactc agcaatggta cctggtattg cttcttcaag ctctttattc
    50221 atttgatgtt cagcaatcca taatgaaagt cttgctactt catgcgcaaa atcatccaat
    50281 tcaatcccat taaaattacc aagatgaatc actgatagtg gcatggaatc attattttgt
    50341 aagtcgccca acaattgcaa aatcttaatt tcaagtctac ggatttcctt ataagcaatg
    50401 atcagaaaat taccagaacc actggcggga tccaaaaact taatattaga aattcgcgtc
    50461 aacaaagcac ttagatcatt gatgattgtt ctcttattat cgtttcttgt tttttcagta
    50521 atgtccttca gctcgttttc ttcactgcga agctttaaag tttcaaatga ctctgtcaaa
    50581 tcatctaaga ataacggctt gatgactttc atgatattcg gcacgcttgt ataatgcata
    50641 ccggcaacgt gacggtcttc tgctgaagca actgattgaa tcattgcgcc aaggatatca
    50701 ggattgattt cattccaatt caataattcc cctgcttcaa taagcagctc tcttgaaact
    50761 tttgaaaagg tcaaatccac atgtggctca ccaaacagtt ttccattaac atatggaaat
    50821 tcaagtagcc actcatcctt gccattgcga gctaactcat taatatccaa gattgcaaag
    50881 agttctttga ttacttcgtt aaaattcgaa ccatcttcag ccgttcttgt ttttagaaca
    50941 tttgtaaagg aacccttatt cataattcct gtatcttctg caaacaacaa gaacagcact
    51001 cgaatcaaga acaagttaaa tgcatgctcg ttggcatcag gattatcttt agccaccaca
    51061 tcatacaatt ttgcaaaacg ttcagcagct tttcggtcag ctggattttc agcttgataa
    51121 tcagccttct caatgccatt ccacgctaag aaaaagtcag cattagccgg tagctcttca
    51181 attggaatat tcaaagtttc atgagttttc gtatcaattg cagcaatatc taaataatcg
    51241 tttaccaaga tatatcgtgg tttagatttt tgctctttga ttgattgagc aatcgcatca
    51301 atcgcaatga caacgttgcc ttcaatttct tcataatgaa gtttgttctt aatcacaaat
    51361 ggttcaccat tatcgaattt tgattttgca cgtgtaatcg acgttttggg gatatcgtac
    51421 aaggacaaaa attgagtgat gaaatcatcg cccctatttg aaagtatttt ttttacacca
    51481 tcttcaattt cagtgatgtt taaaacttta ttttttgcca tttatttaac atcctccgca
    51541 tctataacat ctgaactaat tatttcacta acatcaacat ttaaggcagc agcaattttc
    51601 aataatgact ccattgcaac caactcattt ttaccgagtt ttgctattac attgggactg
    51661 acgccagata tttcacgaag ttgagttttg ttcatattca aatcaatcaa taacttccac
    51721 aatttattat aagaaattga catactacac ctctattcta ctggtatctt caattatatc
    51781 atctatgagc tatcattatt atacgaaaca tgaaatattt tcgcgaacgc aaactatttt
    51841 tctaaaataa aagccttcag catagattta ctaaaggcga gataacaaat attaattcca
    51901 ctttatttta accggaatcc ccaattgacg agctagttta tcaattttga ttctgacatt
    51961 ctttttgttg aaatttgcat aaacccatac tccatcagct accctggctg aatttggtct
    52021 tccgttatac atggatggtt tacgtcctaa aattatagcg aacgacggcg tctcatcgac
    52081 aattaattct tgcagttgat tgttgtagtt tccatccgca aatagctgag aaacaaaatc
    52141 aaccaataaa cctctagcag atttcgaaga tatctttttc ccatcatact caatttcata
    52201 ttgtccagat gaactaaatt cataaatttg ggtttcactg tcttcggaag taccaaacaa
    52261 atcgatattt gcaatttcat ctataatttc aaacactgtg tcatatagct gatccgattt
    52321 tgctttttga agtttatcaa tatacgaagt attaccagca gttgaattca tcatttcata
    52381 gtcagatttt tcttggaaat cttggataag tcgtttttca agataatctg tatctgcttt
    52441 tgacattttc ccatctgctc ttgtaaagaa taaaactgat tcaacccaag ctttatattc
    52501 cttgagaaag tgttgagata aacgaagtga aattgattgc cctgctgcct gacccacgta
    52561 acgcttatta ccaccaatca aaacataaac agctggataa tttgaaaaat cctgttcctt
    52621 caaatacggc aaatcactac gactcattga aaccaagatt gctggctcgc gttcaaaata
    52681 agaaatatta tctttctgtg tatctgaaaa tataaaaatt tgcttcataa gtttgtctcc
    52741 cttgaaaact cgataatata ttatatcacg taaccgttat caaaatcatc taacctatca
    52801 agtctacggt gtaactccta tcaactcaaa gctccatagc atttccatca actctacgct
    52861 cacccaaaaa tcgtcatttc aacacgaaaa aagagcaatc cggaaaccat cccgaatcgc
    52921 tctaatcctt gtcatatcaa cgttccacac gtgtaagcag tcctacacac tcgatgtgat
    52981 gggtttgcgg gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa
    53041 ataacttagc atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg
    53101 gtgacatttc gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa
    53161 ctacgatcac agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt
    53221 ttccgacttc ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt
    53281 caattgcttc tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca
    53341 agccaatcgt acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg
    53401 caaactcgat ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg
    53461 actttgctga gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat
    53521 ataaaacttt ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga
    53581 tgaccgaaac cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa
    53641 agaatttttc tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac
    53701 ggataatgat gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga
    53761 accgttgcaa gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat
    53821 aaaaatcttc gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact
    53881 gtccatcgat acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca
    53941 ttccgaccgt tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct
    54001 ctacttgttt ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga
    54061 tacctatgcg aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa
    54121 atgtttcgac ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt
    54181 cttctccagg gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca
    54241 ttccttcata actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt
    54301 gctgttctgt catattttgc tcctttaagc ctatttttca atcctactga actctactat
    54361 aaatcagcta attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt
    54421 tgattaattc ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc
    54481 atccgttaaa cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc
    54541 atctgtacct gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg
    54601 ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat
    54661 cattactggt cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata
    54721 gattattctt ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa
    54781 gatctcaaag atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt
    54841 ggcatctggc gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat
    54901 cgaagcattt ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac
    54961 tcgagggagc atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag
    55021 ttctgatgga acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa
    55081 gtaactgtta tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc
    55141 tgctgctgct ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt
    55201 accagctggg atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac
    55261 ttcgttgatt gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac
    55321 tctacatgct tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc
    55381 aaatcctgct tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt
    55441 tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc
    55501 taattcatct ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt
    55561 catgatttga ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac
    55621 agattgttgg ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga
    55681 taattgcacc caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa
    55741 ttcacggaag acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat
    55801 cattcctgct aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt
    55861 caagtaagca gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt
    55921 ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc
    55981 atactctgga agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg
    56041 caaactagct tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg
    56101 ctctgggaag taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt
    56161 cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt
    56221 tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt
    56281 tttcaattct gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc
    56341 atcacagcgc atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat
    56401 tgatcgcaat gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga
    56461 aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat
    56521 accatgcatg ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt
    56581 ctttttacct tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg
    56641 ggaaatttga tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt
    56701 atctttagag atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc
    56761 tttattcatt actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt
    56821 tggttctgca ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc
    56881 gacgtggact tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata
    56941 gctggttgtt gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc
    57001 gttttttcat caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa
    57061 cctgccggta cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca
    57121 ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt
    57181 gaagatggcc cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc
    57241 aatgtacgga cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag
    57301 aatgtaccta acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg
    57361 acatatacat catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg
    57421 aatcgttgca agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg
    57481 tattttgaat gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct
    57541 gctgctttca atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta
    57601 ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact
    57661 gaaacgcctg aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat
    57721 gcattatctt tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca
    57781 atcaaaccaa aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa
    57841 gctgctggtt ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca
    57901 gctgcaactg ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa
    57961 gcattttttg ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc
    58021 atgtttagct taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca
    58081 tcataaattg gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt
    58141 gttacgatat tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc
    58201 cctttttcat ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat
    58261 gcttcgacct caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt
    58321 acagtgattt ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat
    58381 tctgtcatta tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt
    58441 cttctgtttc cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat
    58501 cttgtcgcat cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat
    58561 ctacttcttc cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg
    58621 caagttcttc atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt
    58681 cactaatcgc catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta
    58741 acacaagagt gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc
    58801 attcttctac tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt
    58861 cgattcctaa ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga
    58921 tatccgtttt tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt
    58981 ttgcttcttc acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa
    59041 aaggagactc gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc
    59101 ctgcttgttt taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta
    59161 cactgtcggc aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt
    59221 tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga
    59281 catgtcggat tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga
    59341 ttgctgtcaa aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt
    59401 cttctgtcaa tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt
    59461 ctagtacgcg cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca
    59521 aaccttcttt catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat
    59581 ccaaatggac aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc
    59641 tgtctttagg tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt
    59701 tataaatcag aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat
    59761 gcaggctggc acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg
    59821 gcgttactac ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt
    59881 cttctggcgg aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt
    59941 gatcttgtag cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat
    60001 ccacacgttt gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata
    60061 gacgtttttc ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact
    60121 gggtttttgc ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc
    60181 ttttcgctgt gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat
    60241 tcgcaaagat atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt
    60301 ttggcatgaa acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga
    60361 ccgaccggac agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag
    60421 tcgcacctct cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt
    60481 ttagttcaca gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag
    60541 ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga
    60601 ctttttcaaa accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga
    60661 tcaaatcagg atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat
    60721 agtcttccac tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg
    60781 tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt
    60841 accctccact actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct
    60901 tgacttggga aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact
    60961 gttcctagac cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc
    61021 cctccagtag cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct
    61081 gtttttttag cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt
    61141 aactcctgat cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat
    61201 aatgttctgg agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca
    61261 tatgcgagac ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga
    61321 aaaaccccct cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca
    61381 tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca
    61441 agtgccagat catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca
    61501 ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt
    61561 tcgatgcgtg cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta
    61621 cgatccaaaa cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact
    61681 tcttggatca tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc
    61741 agatccacat tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt
    61801 agtttttcga tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa
    61861 ctgattgaat cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct
    61921 ttacgatcgt agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga
    61981 gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa
    62041 ttccgatgat tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct
    62101 cgttcattat ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag
    62161 ttttttggtt ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt
    62221 ttcgtggagc gatagttttg ttctagcaaa attactgcag catctggata atctttttca
    62281 aaatccaaga tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct
    62341 cccacgacgc acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg
    62401 tgatttgtat cttggtactc atctacatga atataatgga atttgttttg ataatatgtg
    62461 agtgaatcag gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc
    62521 atacattgat tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct
    62581 tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct
    62641 tgagagattg ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct
    62701 tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg
    62761 ttatacccga taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc
    62821 gatacccaga catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct
    62881 tttgcagcct tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc
    62941 tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc
    63001 attagcagta atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg
    63061 tttaataatt cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat
    63121 tatagcattt tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat
    63181 ggatttttcc ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga
    63241 atggaaaaaa aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa
    63301 ggaatatttg ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa
    63361 aagctaagcg atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta
    63421 attggcctag ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat
    63481 gaaccggata tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta
    63541 tcaatgaact ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt
    63601 gcgggccttt ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa
    63661 ggagctggac aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt
    63721 gcaagtagcg gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta
    63781 gaagaaatcc atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc
    63841 actgctaact tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac
    63901 cttccttctt tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc
    63961 atcttaggtt ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg
    64021 cctttaccag cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat
    64081 ttatggccta gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc
    64141 ttgccaatca ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct
    64201 tacggtggtg gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc
    64261 ggtagctttt atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat
    64321 tttgtcgtta ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca
    64381 attattttag tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct
    64441 gctcttgttg cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta
    64501 ttggaacgtc tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt
    64561 gattttccag taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg
    64621 cctaaaaata tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt
    64681 gatacgatca tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca
    64741 agaatcaagc aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa
    64801 cgaacatgga aaaactattt tacaacaact attcatctac actacttgat tctatatgat
    64861 tctataaaaa aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct
    64921 gctgctgcag gagcttttct caataaacca taaatcacac cagatacgac tgctccgata
    64981 gcaataaaca ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca
    65041 tgaggagcca gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca
    65101 ataataaaac ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc
    65161 gtgacaaacg atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg
    65221 aatttattct taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca
    65281 gccataacag aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct
    65341 gtaccaaaca cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca
    65401 gctaataatg caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc
    65461 aatgcatcat tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc
    65521 agtaagccaa acactggata aaagaggatc gttttaattc catccaatga ttttggtaag
    65581 ttcttgaata atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt
    65641 gcacctaaga atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc
    65701 aaaccagggc ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa
    65761 ccaaatgcag cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga
    65821 tttgataatt gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata
    65881 gcgattcctc caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg
    65941 taaattcttt gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca
    66001 tcaccatgga atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta
    66061 cggataccat cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct
    66121 acttttttgt ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat
    66181 cggtttttga ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc
    66241 gcttttttct tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct
    66301 gtaacggcaa caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct
    66361 tgttctgctt tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact
    66421 tcttctggtg tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga
    66481 gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg
    66541 aaaaacagat aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt
    66601 gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca
    66661 tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg
    66721 atatcggaaa tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt
    66781 ttatcagtcg cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc
    66841 atgattatcc tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat
    66901 caatcaattc gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc
    66961 cccatttaaa ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa
    67021 tcattgaatc ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat
    67081 aagttccttg ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct
    67141 gtgccccttc agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg
    67201 ttgtattgta aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa
    67261 caagtgcatt ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc
    67321 gaatgatatc aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg
    67381 acaatacgac gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt
    67441 cttcatctga aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt
    67501 tgatattgat tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt
    67561 caagccactt cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt
    67621 gagaatcgat ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga
    67681 agtcttcctt cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat
    67741 caattgatgg attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt
    67801 ttcacggaat tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc
    67861 caaaggtgca acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa
    67921 tgtctcttcg gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt
    67981 cgtgaatcct tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa
    68041 attttttagt tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt
    68101 tccacctaaa ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga
    68161 atttgtcact actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga
    68221 gccagcgtct aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat
    68281 ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa
    68341 taattgctct ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc
    68401 ttgtaaatca cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga
    68461 tttcacgatt ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag
    68521 cattttcacg atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa
    68581 tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa
    68641 aaagactggt tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg
    68701 cttcttgttt ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa
    68761 taaagtccca tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta
    68821 ggaacaaaaa agagacagac ggataataat gatgccggaa ccattattcg ataatagagt
    68881 tgtttatcca ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg
    68941 gtaatctaaa aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact
    69001 tcgccgcttt ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt
    69061 taacggtaat tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc
    69121 ttgatatttt tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc
    69181 ttcgattcca ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga
    69241 gcgattctcg tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct
    69301 ttctttataa tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt
    69361 tttaggaaca ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt
    69421 gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg
    69481 atcaaaccca agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca
    69541 gttaaagggg tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt
    69601 ttctacttgt tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact
    69661 ataatacatt tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg
    69721 aaattggttg caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc
    69781 gatttcatcc tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat
    69841 aaactcgcta taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat
    69901 gaagtctcga atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat
    69961 caataattga ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa
    70021 ccaactttcg tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata
    70081 cggatccgct tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa
    70141 aatagcccaa tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat
    70201 agtcatcaat aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat
    70261 ttaattataa ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac
    70321 gattacatga tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt
    70381 gaacgtacag ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca
    70441 gttgcaccaa aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa
    70501 aaagacaaga aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg
    70561 aaagagtttg actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg
    70621 ctgcccacgc ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat
    70681 tgcgatcaaa atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag
    70741 ggatcttatc tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt
    70801 gatacatctg gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg
    70861 cacgtagctg gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct
    70921 cagttttgga tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct
    70981 cgatcaaata aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat
    71041 tctttcgatc aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca
    71101 gagctatttc tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa
    71161 tttcagcttg atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg
    71221 gctgtcgcaa aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc
    71281 tctgaatctt tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata
    71341 atcacttttt cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct
    71401 tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg
    71461 gctctcaacg cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga
    71521 tattgtttga ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc
    71581 aatttctcta aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat
    71641 tttagtaaat cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg
    71701 ttttgtgtac caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg
    71761 ccatcaaatg caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct
    71821 tgatgtggat gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt
    71881 ttcagcaagg cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg
    71941 cttttacgcg caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc
    72001 acattgtaat cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca
    72061 taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt
    72121 gctaatgttt gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg
    72181 cttttttgca gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag
    72241 tgattgtata cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta
    72301 ttcaatactt caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct
    72361 tcataaaatt cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag
    72421 acccctggac cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag
    72481 cgtacggtct cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa
    72541 agtatgcaag aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa
    72601 aaagaaaaag tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa
    72661 caaaaaataa ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt
    72721 tctagaagta gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc
    72781 gctgtacctg tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct
    72841 tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg
    72901 atttctgcat tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg
    72961 atcacaagat ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct
    73021 tcttctgtag aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg
    73081 ttgttcaaga attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat
    73141 tcaacgattt ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc
    73201 gctgcttttt cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat
    73261 gttgatactt cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt
    73321 gcttttactt catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct
    73381 tgataatctt ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact
    73441 aattgatctt caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg
    73501 ccttttccgc catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta
    73561 tcgccatttt cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca
    73621 cgtttgatac gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact
    73681 agatctttat attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc
    73741 cagtcttgac ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg
    73801 ccagcttctt tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca
    73861 tataaagctt cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg
    73921 aatccaggta cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg
    73981 atttcgtctt gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat
    74041 gttgcagtca tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc
    74101 atgcatacct ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga
    74161 atccttgttt ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc
    74221 atttgctgca aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct
    74281 tcatacatcg tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct
    74341 tcgatgtatg tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt
    74401 aacacggaac taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc
    74461 tggtaacttg cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt
    74521 tcttcaatcg tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt
    74581 aattttgccc ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg
    74641 ataaactcat gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt
    74701 tccaacgaag gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt
    74761 tgtccggata attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca
    74821 taaaaatctg tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac
    74881 aagtcaaaat aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt
    74941 gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta
    75001 ttagcctcaa agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga
    75061 tattgctggt ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga
    75121 aaatcaatcg gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt
    75181 agcaaaacaa aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt
    75241 ttagatctga cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg
    75301 ttgacttcct ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa
    75361 aaatcgccta gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc
    75421 aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta
    75481 agttcttctt catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga
    75541 ccatgattga atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat
    75601 ccgtagatac tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca
    75661 agtgttaaac gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg
    75721 ataaaaaaac ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt
    75781 gtgtccgtat actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc
    75841 tctctttcaa aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc
    75901 tgctgttgct tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact
    75961 cacctccgta ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg
    76021 ataagatctg actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta
    76081 accatgtact agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa
    76141 gctgttgttc ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta
    76201 aacctacttc ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt
    76261 cttcttcaga tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt
    76321 cgatatatgc ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca
    76381 tatctttcaa cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc
    76441 ctgaataatt caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg
    76501 caaagatttt atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca
    76561 tgctgacata cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt
    76621 ccgcactttc gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg
    76681 ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag
    76741 aagcaaataa acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga
    76801 ttttttgcgt actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata
    76861 accaactgtt caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa
    76921 ctaacgaaac cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca
    76981 ttctttctcc tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag
    77041 tgtcgaaccg ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt
    77101 catcttctac cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt
    77161 ggatgctatc ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg
    77221 cttcgaattt ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat
    77281 ccatttttac tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg
    77341 atagataaaa acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata
    77401 taccaattta ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt
    77461 taaatgtttc cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca
    77521 ggtctgtgta aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag
    77581 ggactgcact ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt
    77641 cctgcagctg gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg
    77701 gaattgttgg aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa
    77761 aattagaata ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt
    77821 atctggtcaa agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta
    77881 caggaaatat aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa
    77941 acgcccatcc acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg
    78001 aaaactttga agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg
    78061 atactgagat tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg
    78121 cgtttttaaa agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga
    78181 cagcacctga tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg
    78241 atggattcaa tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg
    78301 ttgagattga agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt
    78361 ttgcaggtga catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg
    78421 agaaaggtac ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc
    78481 gtcgtatcgc tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg
    78541 tcgatacatt gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg
    78601 cggggttagc aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt
    78661 tagccagtga atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct
    78721 tgagtcaaag tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg
    78781 gctacccttc acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct
    78841 ttacactgct attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg
    78901 cacaaatagc tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa
    78961 cttctcttgt attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg
    79021 tcatcgccca aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa
    79081 atgcctttta cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat
    79141 tgaaagaaat cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa
    79201 cgattgcttt gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg
    79261 gtgcgcatac tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta
    79321 ttgtctcaga gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt
    79381 atctaactac tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat
    79441 tacggggatt cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat
    79501 aaaagaacaa ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg
    79561 aaaatatctc ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat
    79621 agtaattaac tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac
    79681 cggaaggacg aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca
    79741 cttttttgat tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta
    79801 atttttgcgg atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt
    79861 tcatgatatt cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta
    79921 agaaaatcat gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt
    79981 ctacaacgta acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg
    80041 ctttgtggaa tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat
    80101 ttttttctgc caagtatcta gcacagataa acatgatttt atcaccgtcg acaatgtttc
    80161 caagttcatc caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg
    80221 ctcctttttc aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat
    80281 tgatatttaa cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa
    80341 acaaacgatt aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac
    80401 cggaaaggtc accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg
    80461 gaaattcttc tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag
    80521 catctaataa tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac
    80581 cgaaaaattt gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg
    80641 cgcttgcttt ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct
    80701 ggaacacttc gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac
    80761 ctgagattct tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct
    80821 ggcttaggac ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct
    80881 tgttggcttc accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac
    80941 tcctttaact taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc
    81001 tgatgtttct tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat
    81061 ggttgttgtc gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt
    81121 attttcttgt gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg
    81181 tgtaatctcc acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc
    81241 aattttacga gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc
    81301 accgatcgat gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact
    81361 gaaatcatag ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac
    81421 cgacttgctt ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat
    81481 acttaatact tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc
    81541 atctgtgacc atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt
    81601 gtcacctgta gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc
    81661 agatggtgtg aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt
    81721 ctcaaccgtt actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt
    81781 ttgaattttt agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac
    81841 gcggaacatc cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga
    81901 aagtttgaca gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga
    81961 aatcttgatt ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt
    82021 tggaacggac tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa
    82081 actgtaaaga atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc
    82141 ataccgtcta ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc
    82201 agttctctac gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta
    82261 tctaatgtaa tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg
    82321 acttcgctta taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata
    82381 ctttctgata gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact
    82441 gcaccgtcat gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata
    82501 tctgcatcta atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata
    82561 gtaattagcg caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag
    82621 atcatcttct catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca
    82681 agatgttcca gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg
    82741 tacgtaatga cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg
    82801 attcggataa caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc
    82861 agcagttgga tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc
    82921 aaaaaattca gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa
    82981 agttccccga tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag
    83041 tatatcatat gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca
    83101 taattttcag gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa
    83161 cttcagagaa ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt
    83221 gttccatttt tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg
    83281 tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt
    83341 tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt
    83401 cttggatagt cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg
    83461 aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt
    83521 ccaaagtcat tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc
    83581 aatagccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca
    83641 aaccatgtgt gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg
    83701 cttcttcaaa agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat
    83761 aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac
    83821 tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat
    83881 tggaatagac ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat
    83941 gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc
    84001 tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc
    84061 gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa
    84121 acatgtgtac caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata
    84181 cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat
    84241 aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt
    84301 cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg
    84361 cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt
    84421 cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca
    84481 agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc
    84541 aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg
    84601 cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac
    84661 ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt
    84721 ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac
    84781 attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc
    84841 gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa
    84901 gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta
    84961 tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg
    85021 ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta
    85081 cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca
    85141 cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga
    85201 tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa
    85261 tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt
    85321 cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat
    85381 agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg
    85441 ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt
    85501 tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga
    85561 tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga
    85621 atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat
    85681 catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat
    85741 caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac
    85801 caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag
    85861 gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat
    85921 gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa
    85981 aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa
    86041 ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt
    86101 cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta
    86161 atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt
    86221 tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt
    86281 tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct
    86341 cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag
    86401 caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag
    86461 caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat
    86521 ttgtcgtcac tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct
    86581 tgtacatatt cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg
    86641 caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta
    86701 ctacttttac tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta
    86761 attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat
    86821 aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc
    86881 atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt
    86941 tcttttattt ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga
    87001 caaacagtct aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg
    87061 tgttttaaca tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc
    87121 ctttatggtt catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg
    87181 tattgccgac aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg
    87241 caattgcagc gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac
    87301 gtgatgcacc ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa
    87361 atattcgata ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca
    87421 gtagataaac taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac
    87481 gaagctgagc aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt
    87541 gatgaatcta tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag
    87601 gagcgtagga caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg
    87661 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta
    87721 ttccgaataa atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa
    87781 gctgagcaaa ttatctccac caaataattt attcatattt ttgacttgcg catactaact
    87841 aattcttaaa ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg
    87901 gtgattcaca ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa
    87961 aaatagttgc ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg
    88021 tttattttaa acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg
    88081 gcaaacacaa atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact
    88141 cctaactcct ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca
    88201 cagcctagta tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact
    88261 ggctgatgaa atggattaat agatttcgat tattcaaagc gattttctat tggtggattg
    88321 tgttcccaac taacaagacg gattccaaag tcattatata actcttcaag agttaaccct
    88381 ttcctgttcc caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg
    88441 ccaagacctg gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa
    88501 atccagctcc tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata
    88561 attgttgtgc cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc
    88621 tacctgcatc tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc
    88681 tttccccatc tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc
    88741 ttggtggctt aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca
    88801 cagctataac aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc
    88861 gcaaatcacc tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc
    88921 tactgatcgt aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca
    88981 tcagctttta cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca
    89041 ccaaaattgt taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga
    89101 tttaacaatt cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat
    89161 tctttgtcat gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca
    89221 ctaggaaaag tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct
    89281 ggaaaataaa atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg
    89341 ctggctaaac gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca
    89401 tcaaattcaa ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca
    89461 agtctaaaag gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa
    89521 ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct
    89581 gctcgaagta aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa
    89641 ataaaaagta ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc
    89701 cctttataaa catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat
    89761 aacgtattcc aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa
    89821 atcactccat ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc
    89881 taatccaatg aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt
    89941 aagaagagca gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc
    90001 cagttataga taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt
    90061 atgtacaaaa ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa
    90121 aacaatagaa gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg
    90181 aagaaaagca agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta
    90241 tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt
    90301 ttgcgaattc ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct
    90361 aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt
    90421 tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta
    90481 gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc
    90541 gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca
    90601 ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca
    90661 ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct
    90721 tggttatgct gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta
    90781 ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt
    90841 cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc
    90901 atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat
    90961 ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa
    91021 tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc
    91081 acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct
    91141 cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc
    91201 tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat
    91261 tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg
    91321 gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact
    91381 agatactcat catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc
    91441 aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca
    91501 tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca
    91561 gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg
    91621 gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact
    91681 tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg
    91741 gttttacggt cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca
    91801 gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt
    91861 tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg
    91921 atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg
    91981 aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca
    92041 ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca
    92101 acagtcacta ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc
    92161 agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt
    92221 ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt
    92281 tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac
    92341 cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca
    92401 atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca
    92461 tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat
    92521 gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt
    92581 taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact
    92641 cattgatttt ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag
    92701 cttgggcttt caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac
    92761 cgtccatcgc cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt
    92821 ctgaagcatc taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca
    92881 gcatgttttg gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt
    92941 ttgtgacttt gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca
    93001 tattatcgat acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg
    93061 caccgctcat acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct
    93121 ctcacattgt gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt
    93181 cttttacaaa aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt
    93241 ttacttttcc gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt
    93301 ccgatctgct aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc
    93361 atgcatcaat gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac
    93421 cgcttcttct aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc
    93481 atgatagatc agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa
    93541 tccaagattt ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag
    93601 aatcgctact ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat
    93661 atgaaggata ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt
    93721 acaagtcaac acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag
    93781 aaacggcatt ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt
    93841 tgcatgattg aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg
    93901 gttgatcaag cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa
    93961 aaaattctcc atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata
    94021 gttatggaac atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa
    94081 cacagtagta ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt
    94141 ttcccgagca tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc
    94201 tgattgattc gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt
    94261 gacgaaacga gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct
    94321 caaattgatt tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt
    94381 ggcaaagcct gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc
    94441 gttataatct ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa
    94501 cagtggatga ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag
    94561 cacacgcata ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata
    94621 accggcttcg tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa
    94681 agcttttttt cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa
    94741 agcaactggg aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc
    94801 tctgacgatc aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct
    94861 tactttattg atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc
    94921 tgacagctct tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa
    94981 aagatatttt ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat
    95041 aaacacaggg tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg
    95101 ggtaggggct aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa
    95161 agaacgtgcc ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc
    95221 attttcaata ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc
    95281 aactacacaa ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt
    95341 gctcaattct tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg
    95401 tagaaagaac taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt
    95461 cattttttta tgttttttta actatctcag ctatctatcc tctttatacc attatttttt
    95521 tcaaatagtg acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg
    95581 caaggatcgt tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg
    95641 ttttgattac atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc
    95701 tttgtaatac atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat
    95761 ctagtatata gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc
    95821 gttgtgcctc accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc
    95881 caacatcaac aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta
    95941 cagcatcttc tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt
    96001 ctaatgtttc agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag
    96061 gcaaaaagtg catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc
    96121 cttttacgtt gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt
    96181 gagcaaataa cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg
    96241 gcgtccggcc gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg
    96301 aaattgtctt gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt
    96361 tctttaagat actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta
    96421 caaattctcc taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag
    96481 tgatcttgat tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt
    96541 tccctgaaag atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc
    96601 ctgcagccac gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat
    96661 ctgctgccca catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac
    96721 gcattttctt taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg
    96781 gttcatctaa aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga
    96841 ttcgctgcgc ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta
    96901 gtcccacatt ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa
    96961 ttactttctc ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag
    97021 acaactcatt tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat
    97081 tcaatcgata acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct
    97141 cacgcgtgaa atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt
    97201 cgaaagtcgt ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa
    97261 gatcatctgt cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg
    97321 tatctaaatc gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag
    97381 agctgattgg attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa
    97441 tcactaaatc ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc
    97501 caaaaggcgc attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat
    97561 aaggacaggc atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag
    97621 catagccgtc tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc
    97681 cttctttcac aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca
    97741 gctcaggcac ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct
    97801 ctttttggat tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg
    97861 ccaacacttg gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta
    97921 ctgattgact ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa
    97981 acaacaagcg taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt
    98041 tacttgttgt tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt
    98101 ccggcttatc catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc
    98161 tccgctgccc ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca
    98221 aacctgtaac gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat
    98281 gggcacgtgc accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag
    98341 cttttaattc taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg
    98401 ccgcattttt catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca
    98461 tttcttcata cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag
    98521 aaatcaaatc tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt
    98581 acttctgctg aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg
    98641 ttactttgtc tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg
    98701 tttggactaa ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa
    98761 caagggaaac ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat
    98821 caaattcacc taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac
    98881 tatgcaaata tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca
    98941 tttttttcgt caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt
    99001 cgcctactag atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca
    99061 gccctgtcgg acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc
    99121 ctggcgtcgc agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa
    99181 gtggccgatt gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc
    99241 tcgctcggtc accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg
    99301 ccacgatcaa aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt
    99361 tacgtccgtc catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca
    99421 tcatttcaat atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt
    99481 catttcgcaa gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct
    99541 ccatatgttc atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt
    99601 ctcctaaaac ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa
    99661 aaaactccac acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac
    99721 cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga
    99781 ctaagtcacg aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt
    99841 gtttttggta ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga
    99901 tcacatcatt tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat
    99961 catttacact tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct
   100021 gataataatc ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga
   100081 attccccgta cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat
   100141 tcactttctc aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata
   100201 agatttgtgc ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct
   100261 gatcaccagc cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa
   100321 tcattcttcg tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag
   100381 gaaagcaaag gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt
   100441 tttatcttat tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt
   100501 cattttttcg agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata
   100561 ttggaaattt aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt
   100621 aatgacagga cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac
   100681 ggcagagtca agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt
   100741 aattgccagt gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga
   100801 aggcatcgat gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat
   100861 ggactaccgt ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat
   100921 gattgccgga atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta
   100981 ctttgatagc gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga
   101041 agatttgaaa ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag
   101101 cgaccatgaa tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta
   101161 cgacgggtta aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta
   101221 tgcgatcaaa caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata
   101281 tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt
   101341 gaaagaatta aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg
   101401 ttcagaaact tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca
   101461 aaacaactat caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc
   101521 ctttgctttg gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg
   101581 cgctcttcgc acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt
   101641 tcttgcgttc tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt
   101701 ccttgctggt gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg
   101761 aggcgggatc aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc
   101821 ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc
   101881 attcgtcaat cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt
   101941 agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt
   102001 ttacttgatc attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa
   102061 agttttagaa aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca
   102121 aaaaatacgg cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg
   102181 tcgtctgtat cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc
   102241 gtttagaaga accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa
   102301 gcacagatat caacttggtg agacaacata tcggaatggt attccagcac tttaacctat
   102361 tccctcatct ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat
   102421 ctagagaaga agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata
   102481 aaaaagatat gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc
   102541 gtgctctagc gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc
   102601 ccgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga
   102661 tggttatcgt tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca
   102721 ttgacgacgg aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa
   102781 atgaacggac aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc
   102841 ccggtcgctt catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca
   102901 gccgcatcgc ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag
   102961 aaagtaccat cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta
   103021 tgatgattat cttattctaa agctggcttt tttgttggta caaacatgac aactac
//
LOCUS       DAYYCM010000002        91889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000002 DAYYCM010000000
VERSION     DAYYCM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.002"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1582)
                     /locus_tag="ACHAY1_000096"
     CDS             complement(8..1582)
                     /locus_tag="ACHAY1_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8592131.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="ACHAY1_000097"
     CDS             complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="ACHAY1_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8592132.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="ACHAY1_000098"
     CDS             complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="ACHAY1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8592133.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3359..3997)
                     /locus_tag="ACHAY1_000099"
     CDS             complement(3359..3997)
                     /locus_tag="ACHAY1_000099"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8592134.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3998..4648)
                     /gene="rpe"
                     /locus_tag="ACHAY1_000100"
     CDS             complement(3998..4648)
                     /gene="rpe"
                     /locus_tag="ACHAY1_000100"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8592135.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="ACHAY1_000101"
     CDS             complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="ACHAY1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8592136.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="ACHAY1_000102"
     CDS             complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="ACHAY1_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8592137.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7791..8531)
                     /locus_tag="ACHAY1_000103"
     CDS             complement(7791..8531)
                     /locus_tag="ACHAY1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8592138.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="ACHAY1_000104"
     CDS             complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="ACHAY1_000104"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8592139.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9902..10840)
                     /gene="fmt"
                     /locus_tag="ACHAY1_000105"
     CDS             complement(9902..10840)
                     /gene="fmt"
                     /locus_tag="ACHAY1_000105"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8592140.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10833..11324)
                     /gene="def"
                     /locus_tag="ACHAY1_000106"
     CDS             complement(10833..11324)
                     /gene="def"
                     /locus_tag="ACHAY1_000106"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8592141.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11339..13747)
                     /gene="priA"
                     /locus_tag="ACHAY1_000107"
     CDS             complement(11339..13747)
                     /gene="priA"
                     /locus_tag="ACHAY1_000107"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8592142.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(14000..14305)
                     /gene="rpoZ"
                     /locus_tag="ACHAY1_000108"
     CDS             complement(14000..14305)
                     /gene="rpoZ"
                     /locus_tag="ACHAY1_000108"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8592143.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="ACHAY1_000109"
     CDS             complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="ACHAY1_000109"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8592144.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15411..15695)
                     /locus_tag="ACHAY1_000110"
     CDS             complement(15411..15695)
                     /locus_tag="ACHAY1_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592145.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15713..16603)
                     /locus_tag="ACHAY1_000111"
     CDS             complement(15713..16603)
                     /locus_tag="ACHAY1_000111"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8592146.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16623..16841)
                     /locus_tag="ACHAY1_000112"
     CDS             complement(16623..16841)
                     /locus_tag="ACHAY1_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8592147.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16811..18511)
                     /locus_tag="ACHAY1_000113"
     CDS             complement(16811..18511)
                     /locus_tag="ACHAY1_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8592148.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18778..19551
                     /locus_tag="ACHAY1_000114"
     CDS             18778..19551
                     /locus_tag="ACHAY1_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592149.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="ACHAY1_000115"
     CDS             complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="ACHAY1_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8592150.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20267..20369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20375..21346)
                     /locus_tag="ACHAY1_000116"
     CDS             complement(20375..21346)
                     /locus_tag="ACHAY1_000116"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8592151.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="ACHAY1_000117"
     CDS             complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="ACHAY1_000117"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8592152.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23316..24134)
                     /gene="purR"
                     /locus_tag="ACHAY1_000118"
     CDS             complement(23316..24134)
                     /gene="purR"
                     /locus_tag="ACHAY1_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8592153.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24301..25125)
                     /locus_tag="ACHAY1_000119"
     CDS             complement(24301..25125)
                     /locus_tag="ACHAY1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8592154.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25098..25778)
                     /locus_tag="ACHAY1_000120"
     CDS             complement(25098..25778)
                     /locus_tag="ACHAY1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8592155.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25801..26739)
                     /locus_tag="ACHAY1_000121"
     CDS             complement(25801..26739)
                     /locus_tag="ACHAY1_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8592156.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26984..27835)
                     /gene="ispE"
                     /locus_tag="ACHAY1_000122"
     CDS             complement(26984..27835)
                     /gene="ispE"
                     /locus_tag="ACHAY1_000122"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8592157.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="ACHAY1_000123"
     CDS             complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="ACHAY1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8592158.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30703..31509
                     /gene="proC"
                     /locus_tag="ACHAY1_000124"
     CDS             30703..31509
                     /gene="proC"
                     /locus_tag="ACHAY1_000124"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8592159.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31588..33144)
                     /gene="rny"
                     /locus_tag="ACHAY1_000125"
     CDS             complement(31588..33144)
                     /gene="rny"
                     /locus_tag="ACHAY1_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8592160.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33555..34604)
                     /gene="recA"
                     /locus_tag="ACHAY1_000126"
     CDS             complement(33555..34604)
                     /gene="recA"
                     /locus_tag="ACHAY1_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8592161.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34713..35954)
                     /locus_tag="ACHAY1_000127"
     CDS             complement(34713..35954)
                     /locus_tag="ACHAY1_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8592162.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="ACHAY1_000128"
     CDS             complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="ACHAY1_000128"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8592163.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36981..37904)
                     /locus_tag="ACHAY1_000129"
     CDS             complement(36981..37904)
                     /locus_tag="ACHAY1_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8592164.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38643..39929)
                     /locus_tag="ACHAY1_000130"
     CDS             complement(38643..39929)
                     /locus_tag="ACHAY1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8592165.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39922..41190)
                     /locus_tag="ACHAY1_000131"
     CDS             complement(39922..41190)
                     /locus_tag="ACHAY1_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8592166.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41442..41903
                     /gene="mscL"
                     /locus_tag="ACHAY1_000132"
     CDS             41442..41903
                     /gene="mscL"
                     /locus_tag="ACHAY1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8592167.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41962..42408)
                     /locus_tag="ACHAY1_000133"
     CDS             complement(41962..42408)
                     /locus_tag="ACHAY1_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592168.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42501..42746)
                     /locus_tag="ACHAY1_000134"
     CDS             complement(42501..42746)
                     /locus_tag="ACHAY1_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8592169.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42861..43439)
                     /locus_tag="ACHAY1_000135"
     CDS             complement(42861..43439)
                     /locus_tag="ACHAY1_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8592170.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43516..44046)
                     /locus_tag="ACHAY1_000136"
     CDS             complement(43516..44046)
                     /locus_tag="ACHAY1_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8592171.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="ACHAY1_000137"
     CDS             complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="ACHAY1_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8592172.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44903..45298)
                     /locus_tag="ACHAY1_000138"
     CDS             complement(44903..45298)
                     /locus_tag="ACHAY1_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8592173.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45295..46707)
                     /gene="cysS"
                     /locus_tag="ACHAY1_000139"
     CDS             complement(45295..46707)
                     /gene="cysS"
                     /locus_tag="ACHAY1_000139"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8592174.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46704..47240)
                     /gene="epsC"
                     /locus_tag="ACHAY1_000140"
     CDS             complement(46704..47240)
                     /gene="epsC"
                     /locus_tag="ACHAY1_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8592175.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47326..47565)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47562..49022)
                     /gene="gltX"
                     /locus_tag="ACHAY1_000141"
     CDS             complement(47562..49022)
                     /gene="gltX"
                     /locus_tag="ACHAY1_000141"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8592176.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49192..50349)
                     /locus_tag="ACHAY1_000142"
     CDS             complement(49192..50349)
                     /locus_tag="ACHAY1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8592177.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50383..51753)
                     /gene="radA"
                     /locus_tag="ACHAY1_000143"
     CDS             complement(50383..51753)
                     /gene="radA"
                     /locus_tag="ACHAY1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8592178.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51816..52343)
                     /locus_tag="ACHAY1_000144"
     CDS             complement(51816..52343)
                     /locus_tag="ACHAY1_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8592179.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52573..53379
                     /gene="proB"
                     /locus_tag="ACHAY1_000145"
     CDS             52573..53379
                     /gene="proB"
                     /locus_tag="ACHAY1_000145"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8592180.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53376..54623
                     /locus_tag="ACHAY1_000146"
     CDS             53376..54623
                     /locus_tag="ACHAY1_000146"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8592181.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54699..55043
                     /locus_tag="ACHAY1_000147"
     CDS             54699..55043
                     /locus_tag="ACHAY1_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592182.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55160..55486)
                     /locus_tag="ACHAY1_000148"
     CDS             complement(55160..55486)
                     /locus_tag="ACHAY1_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592183.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56101..57303)
                     /locus_tag="ACHAY1_000149"
     CDS             complement(56101..57303)
                     /locus_tag="ACHAY1_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8592184.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57420..57935
                     /locus_tag="ACHAY1_000150"
     CDS             57420..57935
                     /locus_tag="ACHAY1_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8592185.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58134..58580)
                     /locus_tag="ACHAY1_000151"
     CDS             complement(58134..58580)
                     /locus_tag="ACHAY1_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8592186.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="ACHAY1_000152"
     CDS             complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="ACHAY1_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8592187.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60459..61088)
                     /locus_tag="ACHAY1_000153"
     CDS             complement(60459..61088)
                     /locus_tag="ACHAY1_000153"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8592188.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61072..61536)
                     /locus_tag="ACHAY1_000154"
     CDS             complement(61072..61536)
                     /locus_tag="ACHAY1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8592189.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62023..63291)
                     /locus_tag="ACHAY1_000155"
     CDS             complement(62023..63291)
                     /locus_tag="ACHAY1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8592190.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63522..64259)
                     /locus_tag="ACHAY1_000156"
     CDS             complement(63522..64259)
                     /locus_tag="ACHAY1_000156"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8592191.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64326..64895)
                     /locus_tag="ACHAY1_000157"
     CDS             complement(64326..64895)
                     /locus_tag="ACHAY1_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8592192.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65023..65895
                     /locus_tag="ACHAY1_000158"
     CDS             65023..65895
                     /locus_tag="ACHAY1_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8592193.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66237..67109
                     /locus_tag="ACHAY1_000159"
     CDS             66237..67109
                     /locus_tag="ACHAY1_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8592194.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67106..69064
                     /gene="nagE"
                     /locus_tag="ACHAY1_000160"
     CDS             67106..69064
                     /gene="nagE"
                     /locus_tag="ACHAY1_000160"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8592195.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69396..70247)
                     /locus_tag="ACHAY1_000161"
     CDS             complement(69396..70247)
                     /locus_tag="ACHAY1_000161"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8592196.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70370..70837
                     /locus_tag="ACHAY1_000162"
     CDS             70370..70837
                     /locus_tag="ACHAY1_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8592197.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71100..71582)
                     /locus_tag="ACHAY1_000163"
     CDS             complement(71100..71582)
                     /locus_tag="ACHAY1_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8592198.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71682..72545)
                     /locus_tag="ACHAY1_000164"
     CDS             complement(71682..72545)
                     /locus_tag="ACHAY1_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8592199.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            73143..74018
                     /locus_tag="ACHAY1_000165"
     CDS             73143..74018
                     /locus_tag="ACHAY1_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8592200.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(74163..74315)
                     /locus_tag="ACHAY1_000166"
                     /pseudo
     CDS             complement(74163..74315)
                     /locus_tag="ACHAY1_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(74583..75914)
                     /locus_tag="ACHAY1_000167"
     CDS             complement(74583..75914)
                     /locus_tag="ACHAY1_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8592201.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(76063..76776)
                     /locus_tag="ACHAY1_000168"
     CDS             complement(76063..76776)
                     /locus_tag="ACHAY1_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8592202.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            76951..78375
                     /locus_tag="ACHAY1_000169"
     CDS             76951..78375
                     /locus_tag="ACHAY1_000169"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8592203.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            78674..79381
                     /locus_tag="ACHAY1_000170"
     CDS             78674..79381
                     /locus_tag="ACHAY1_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8592204.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            79368..79691
                     /locus_tag="ACHAY1_000171"
     CDS             79368..79691
                     /locus_tag="ACHAY1_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8592205.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(79780..80817)
                     /locus_tag="ACHAY1_000172"
     CDS             complement(79780..80817)
                     /locus_tag="ACHAY1_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8592206.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(81138..82121)
                     /locus_tag="ACHAY1_000173"
     CDS             complement(81138..82121)
                     /locus_tag="ACHAY1_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8592207.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(82536..83306)
                     /locus_tag="ACHAY1_000174"
     CDS             complement(82536..83306)
                     /locus_tag="ACHAY1_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592208.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(83299..84234)
                     /locus_tag="ACHAY1_000175"
     CDS             complement(83299..84234)
                     /locus_tag="ACHAY1_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592209.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(84550..86199)
                     /locus_tag="ACHAY1_000176"
     CDS             complement(84550..86199)
                     /locus_tag="ACHAY1_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8592210.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(86316..88919)
                     /locus_tag="ACHAY1_000177"
     CDS             complement(86316..88919)
                     /locus_tag="ACHAY1_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8592211.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(88931..89980)
                     /locus_tag="ACHAY1_000178"
     CDS             complement(88931..89980)
                     /locus_tag="ACHAY1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8592212.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(90006..90320)
                     /locus_tag="ACHAY1_000179"
     CDS             complement(90006..90320)
                     /locus_tag="ACHAY1_000179"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8592213.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(90336..90782)
                     /locus_tag="ACHAY1_000180"
     CDS             complement(90336..90782)
                     /locus_tag="ACHAY1_000180"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8592214.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(90783..>91889)
                     /locus_tag="ACHAY1_000181"
     CDS             complement(90783..>91889)
                     /locus_tag="ACHAY1_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8592215.1"
                     /translation="VPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT        26206 a        19441 c        16193 g        30049 t
ORIGIN      
        1 atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa ccaactttta
       61 cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca atcgctacgt
      121 ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct tgagaaactg
      181 aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca cgaccagtta
      241 cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga tctttaccgc
      301 cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct gcaccattca
      361 cacttccaga aggagctgga gcaggtgtta caggttttgg ttgttcaggt gttggtgttg
      421 gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt tgatctgttg
      481 atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca ggcactgttg
      541 ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt
      601 caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat tcttctgttg
      661 aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat gatgattctt
      721 cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt gcagtagatg
      781 aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt tcagcttttt
      841 cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga atacgagctt
      901 gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct tcagcagttg
      961 cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta gataaaagat
     1021 cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt ttagcatcgt
     1081 tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc atcaagtcgt
     1141 tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct gctaatgaat
     1201 cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg
     1261 cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct gcaatgtctt
     1321 ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt
     1381 gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt gcatctgctt
     1441 gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa tcaaaatcgt
     1501 ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat accattactg
     1561 ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta ataaatatct
     1621 aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg tgtcaacgat
     1681 ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa aactgttcgt
     1741 ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga taacaacgat
     1801 tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga ataatttctt
     1861 aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag gtcctaaaaa
     1921 ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact ggaccaccat
     1981 cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag taaagatgtt
     2041 gacatggata acgtctttca ttacatacat caaccataca attaaaggga gagctactgc
     2101 atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa caatcgtggt
     2161 aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca atatcagaaa
     2221 gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg ttaaatgacc
     2281 atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg tttctttctt
     2341 caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca cgtcatacat
     2401 ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg gtttcgtttt
     2461 aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg atgtctggac
     2521 aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta aggcatggag
     2581 cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac
     2641 tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag acgaagccgt
     2701 attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca tatttgcttc
     2761 gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt caggcgaacg
     2821 agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca actcaagttc
     2881 ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa cgtcatcata
     2941 gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa gattatggac
     3001 agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat caaccattgc
     3061 aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg ctcgctgcgc
     3121 agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa taataatatt
     3181 tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc ttgtgttcaa
     3241 ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct tttttttagc
     3301 aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg tttatggatc
     3361 aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct gcggtatctg
     3421 aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt tccaatacat
     3481 acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa gctaaatact
     3541 tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac aaataatttt
     3601 gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc atacctaacc
     3661 agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg
     3721 cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta tcgtcttttt
     3781 ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc tcttcgttcg
     3841 acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga agcaggtaca
     3901 gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac tgcggccagt
     3961 tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc tcttttaatc
     4021 gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca acgaagacat
     4081 ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca tctacttcga
     4141 ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt ttttctactg
     4201 tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact agtacttgat
     4261 cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg ataactactc
     4321 cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga ggagttgctt
     4381 cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg tagttttctg
     4441 gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg attgcctgaa
     4501 caatatttgg ccctaatgtg atatttggga caaatcggcc atccattaca tctacgtgga
     4561 tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg gcaaaatctg
     4621 cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt ttaccttctt
     4681 tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa taattgtcgt
     4741 aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag ccaggttctt
     4801 tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat tcaggaaatt
     4861 gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat cctggtgtat
     4921 ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta tgccgtcctc
     4981 ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat atttgattca
     5041 gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt tctgggaagt
     5101 aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga tagcctattt
     5161 tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag tcggtctttg
     5221 tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag cggtcaagta
     5281 gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga tcaacatttg
     5341 tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga tagccgtctg
     5401 tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt ttccgattcc
     5461 ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca tagtaaaaac
     5521 cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt gtttatttta
     5581 ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat atggcttgaa
     5641 aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat gtcgcattcc
     5701 cttctgatat attttattca gcacttattt tgaactttct gaggtgctag ctgatgaact
     5761 tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc tacttgagct
     5821 gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg gtcctttaga
     5881 gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg gtactgtacg
     5941 aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc cgatatattc
     6001 tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg agatatcatt
     6061 caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt ctgctacaac
     6121 ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt cgctatagtc
     6181 ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat tacgaacagt
     6241 ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt tgctgactgt
     6301 gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag attgcgaaat
     6361 gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca agtcagcaga
     6421 aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac tgtaatcatc
     6481 taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt tttgcttgac
     6541 tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt tgtcatctgc
     6601 aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt ctctagcaga
     6661 agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc cagaagcaaa
     6721 gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca gccaccattt
     6781 tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta aggtttcgtt
     6841 ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg gtgcatcttc
     6901 cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc atttttcttc
     6961 gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct cggctgactt
     7021 ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg
     7081 cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat
     7141 tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat aaaggatgat
     7201 ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct gctcagggga
     7261 cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc
     7321 gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca gtacgttttg
     7381 gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag cagaaaggat
     7441 ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt ggatatatcg
     7501 tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt cttcttcacc
     7561 aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta aagcttctct
     7621 ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc
     7681 tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac caccgctacc
     7741 gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga tcatacactg
     7801 tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc ttcattggca
     7861 cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat gatcgttcct
     7921 atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag tcgatctttt
     7981 agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc gacagaacgt
     8041 gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc tcctgatttg
     8101 actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc ttcacgaatc
     8161 aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga cagagcggcc
     8221 gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg ctgataaatc
     8281 agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc atcgtcaaca
     8341 gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct
     8401 ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc tgaacggctg
     8461 gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat
     8521 tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa aaatccatct
     8581 gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga ttgtagcata
     8641 ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca
     8701 acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat accggaagac
     8761 tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa attcgctaat
     8821 tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc aagacctgaa
     8881 caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt actgacttct
     8941 ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc tgcattatct
     9001 tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc accgcatgac
     9061 ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag
     9121 acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg
     9181 atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt tttcgctact
     9241 ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg caaaacagtg
     9301 atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg gctaggctga
     9361 tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac cagtcgtttc
     9421 gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc agcaatgcct
     9481 tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac aaatttccct
     9541 gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca
     9601 gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat cttgacccag
     9661 tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga
     9721 tggctaatcg taccatagat caattctgta aataaaccca catctttttg atttagctcg
     9781 ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt gttcactcgc
     9841 tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg gatttttttc
     9901 attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg aaatcatgta
     9961 cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg gtctgtttac
    10021 cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt gctttgtctg
    10081 tagtttctgc caatacttca accgataata atttccagcg tatgccctca taggtcgtga
    10141 atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct gcagttttat
    10201 gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct tctgactcgt
    10261 cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc aatagctttc
    10321 ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta gtgattggta
    10381 tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc atgatcgtta
    10441 caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct ccccgatatt
    10501 taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc aataactttt
    10561 caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat tcaatgattt
    10621 cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata ccgtgcttca
    10681 gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg
    10741 gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca agaatcggca
    10801 cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc ctccatatat
    10861 tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa gataccgtct
    10921 aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt cacttctatc
    10981 tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt gaccgttcca
    11041 tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt tgcttcgatg
    11101 atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac agcaattctc
    11161 ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat cattgtctca
    11221 tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg ctgagcagtt
    11281 ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg gccctttctt
    11341 aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc tgttctttct
    11401 ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt ttatatttga
    11461 tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc gttggcccta
    11521 atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga aagattttct
    11581 ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag taataaggag
    11641 gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca taattttgtt
    11701 ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg acttcgcctg
    11761 ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat gtatgctcac
    11821 tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca actaatgtca
    11881 cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata tctgcttctt
    11941 ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg gtatctacat
    12001 ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct actttttgag
    12061 tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga ttgggaatag
    12121 gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc agtgtaagag
    12181 agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc acaaaagacg
    12241 aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat cgattttgta
    12301 ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac tcttcacgca
    12361 tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg tttaactgca
    12421 ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc
    12481 ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct ctcgcatgat
    12541 aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata atgatcagcc
    12601 cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact tgcgcttctt
    12661 cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga
    12721 ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga gtcaatgcga
    12781 tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg attgcctgaa
    12841 gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc tgatcttttt
    12901 gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca ttcagttcca
    12961 atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt tctgattcta
    13021 taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg attcctttat
    13081 ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa agaagttctt
    13141 tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc ttttcagtta
    13201 acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact tcatatttga
    13261 tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct tcttcttctg
    13321 cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct ggtgtgtctg
    13381 ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc atcgtttgta
    13441 gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat gaaagtaatt
    13501 cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa gatggattag
    13561 ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga ttgccattac
    13621 caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca gagggaacca
    13681 aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact tcagctgtta
    13741 atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa aaagttcctt
    13801 gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct aacaaaaaat
    13861 taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc cttttctacc
    13921 agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc tttttagatg
    13981 ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat tccaattcgt
    14041 gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt
    14101 ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct aaagcttgac
    14161 cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca tccaattcat
    14221 gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga tctaatagtg
    14281 agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt aatatattta
    14341 ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat tcgttcaact
    14401 gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat ttcgatttct
    14461 tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc tcggcctacg
    14521 atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac accatcgggc
    14581 actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac atcttttcct
    14641 tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc gacatattct
    14701 gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt gcttcggaag
    14761 aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat tgagatcgaa
    14821 tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc ttttcctaca
    14881 ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac gtcctttcaa
    14941 ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt caaggttatc
    15001 tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc
    15061 tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt ttttcgtttg
    15121 gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa
    15181 gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac atttttgcat
    15241 aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag
    15301 attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg
    15361 aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg ctaaaagccc
    15421 atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct
    15481 gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg
    15541 taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct
    15601 ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca
    15661 cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa
    15721 tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt
    15781 taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga
    15841 tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca tctagttttt
    15901 gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg
    15961 ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt
    16021 tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta
    16081 gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt
    16141 tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg
    16201 ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagca
    16261 cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa
    16321 ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt
    16381 tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact
    16441 gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta
    16501 tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt
    16561 ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct
    16621 cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg
    16681 cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga
    16741 ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata
    16801 gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat
    16861 aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac
    16921 gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt
    16981 gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt
    17041 gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca
    17101 tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca
    17161 tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc
    17221 aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca
    17281 aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat
    17341 agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc
    17401 tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga
    17461 taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa
    17521 atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac
    17581 cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag
    17641 aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt
    17701 ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga
    17761 gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc
    17821 cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt
    17881 cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata
    17941 aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg
    18001 aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa
    18061 tctgataagc accgtgcgct cctcctctac ctaaaacaag tgctgtatga tagaccagct
    18121 ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt
    18181 ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag
    18241 cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat
    18301 taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata
    18361 ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat
    18421 gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt
    18481 tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca
    18541 gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa
    18601 ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag
    18661 agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt
    18721 tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg
    18781 actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg
    18841 actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat
    18901 ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca
    18961 gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag
    19021 tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa
    19081 ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag
    19141 aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta
    19201 gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat
    19261 gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg
    19321 aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg
    19381 atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa
    19441 ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga
    19501 cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc
    19561 ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc
    19621 ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt
    19681 tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc
    19741 attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc
    19801 actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg
    19861 atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc
    19921 atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg
    19981 acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc
    20041 agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat
    20101 gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct
    20161 gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg
    20221 aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca
    20281 caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc
    20341 agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg
    20401 gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac
    20461 ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa
    20521 ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat
    20581 gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg
    20641 tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt
    20701 gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg
    20761 caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg
    20821 gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa
    20881 gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat
    20941 gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt
    21001 tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag
    21061 gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca
    21121 actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc
    21181 cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg
    21241 attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat
    21301 ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt
    21361 tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc
    21421 aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat
    21481 tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg
    21541 acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag
    21601 aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga
    21661 cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca ccaacataag
    21721 tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc
    21781 cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat
    21841 ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct
    21901 caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg
    21961 tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg
    22021 catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt
    22081 taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca
    22141 ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga
    22201 tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta
    22261 acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa
    22321 gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt
    22381 cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac
    22441 ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg
    22501 gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt
    22561 gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc
    22621 ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt
    22681 tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta
    22741 aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat
    22801 aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta
    22861 tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa
    22921 aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc
    22981 ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg
    23041 acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc
    23101 ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta
    23161 atactcagga caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt
    23221 tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct
    23281 tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata atttcctggc
    23341 acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa
    23401 tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt
    23461 tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg
    23521 aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc
    23581 ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt
    23641 atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg
    23701 gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat
    23761 ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag
    23821 agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat
    23881 tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca
    23941 gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt
    24001 cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag
    24061 gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg
    24121 cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta cattatataa
    24181 gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt
    24241 aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat
    24301 ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa
    24361 caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct
    24421 agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt
    24481 gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa
    24541 aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat
    24601 catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag
    24661 aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc
    24721 taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat
    24781 acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc
    24841 agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc
    24901 caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa
    24961 aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga
    25021 ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc
    25081 ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt
    25141 ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc
    25201 attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt
    25261 ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta
    25321 gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc
    25381 cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct
    25441 ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg
    25501 atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata
    25561 cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc
    25621 agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa
    25681 atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca
    25741 tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac
    25801 ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc
    25861 gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac
    25921 tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat
    25981 cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg
    26041 ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa
    26101 ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc
    26161 ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa
    26221 agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt
    26281 tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc
    26341 ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc
    26401 tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa
    26461 ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt
    26521 tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc
    26581 ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt
    26641 caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc
    26701 acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa
    26761 caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt
    26821 caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg
    26881 gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt
    26941 taggattcct cagtttttca tcacctattt tacttcttat attttattgc agggttcgta
    27001 ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat
    27061 aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc
    27121 cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat
    27181 cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt
    27241 cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg
    27301 tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag
    27361 gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt
    27421 aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca
    27481 gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta
    27541 atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt
    27601 atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa
    27661 atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat
    27721 ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat
    27781 ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct
    27841 cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc
    27901 ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct
    27961 tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac
    28021 tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga
    28081 aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt
    28141 tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca
    28201 tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc
    28261 ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat
    28321 agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct
    28381 tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac
    28441 cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc
    28501 gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca
    28561 gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct
    28621 gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg
    28681 tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg
    28741 caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca
    28801 ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac
    28861 caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc
    28921 gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt
    28981 tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta
    29041 aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa
    29101 acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg
    29161 caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc
    29221 aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa
    29281 tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac
    29341 caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc
    29401 tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga
    29461 atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg
    29521 atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa
    29581 ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat
    29641 gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat
    29701 ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt cctaagatca
    29761 tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag
    29821 caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga
    29881 tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa
    29941 gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga
    30001 aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac
    30061 tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag
    30121 tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct
    30181 ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt
    30241 tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc
    30301 cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca
    30361 gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc
    30421 aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta
    30481 acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca
    30541 tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac
    30601 cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg
    30661 atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc
    30721 ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg
    30781 aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa
    30841 actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt
    30901 ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag
    30961 aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga aacagcgctt
    31021 gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca
    31081 ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg
    31141 atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc
    31201 attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg
    31261 actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt
    31321 atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa
    31381 gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc
    31441 atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat
    31501 aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt
    31561 gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg
    31621 aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat
    31681 atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg
    31741 aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat
    31801 attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg
    31861 tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc
    31921 cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc
    31981 agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc
    32041 aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc
    32101 taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact
    32161 agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc
    32221 gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga
    32281 tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc
    32341 caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc
    32401 cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg
    32461 accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac
    32521 agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt
    32581 cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa
    32641 ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga
    32701 taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt
    32761 ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga
    32821 gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa
    32881 acgattctct tgagatttta attctaactt actttctctc agctcacttt caacttcaga
    32941 acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc
    33001 ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt
    33061 ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat
    33121 agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc
    33181 atctatacag cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat
    33241 gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa
    33301 ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca
    33361 ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta
    33421 gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct
    33481 ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt
    33541 gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct
    33601 gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc
    33661 atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga
    33721 tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg
    33781 tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt
    33841 ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata
    33901 tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat
    33961 ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca
    34021 cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta
    34081 cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg
    34141 tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc
    34201 ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa
    34261 agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc
    34321 tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt
    34381 gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg
    34441 taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt
    34501 tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt
    34561 tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt
    34621 atatttacaa atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa
    34681 gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa
    34741 agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga
    34801 tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat
    34861 tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt
    34921 gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat
    34981 tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta
    35041 acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag
    35101 gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc
    35161 actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt
    35221 tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt
    35281 ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg
    35341 atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa
    35401 aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga
    35461 cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac
    35521 gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga
    35581 accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt
    35641 gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg
    35701 aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat
    35761 aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga
    35821 ttatctccta caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac
    35881 aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt
    35941 atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt
    36001 ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa
    36061 taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa
    36121 agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc
    36181 cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca
    36241 cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac
    36301 cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag
    36361 ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta
    36421 aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg
    36481 cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg
    36541 ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta
    36601 accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca
    36661 gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga
    36721 aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga
    36781 ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt
    36841 tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa
    36901 gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat
    36961 gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac attttttgga
    37021 ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca
    37081 ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc
    37141 tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct
    37201 aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata
    37261 tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca
    37321 cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag
    37381 gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt
    37441 ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca
    37501 accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc
    37561 tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat
    37621 cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca
    37681 gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa
    37741 tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca
    37801 tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa
    37861 gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa
    37921 agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa
    37981 cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct
    38041 gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt
    38101 aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag
    38161 atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
    38221 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta
    38281 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    38341 cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat
    38401 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    38461 cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat
    38521 ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt
    38581 ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg
    38641 gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca
    38701 gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt
    38761 cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat
    38821 tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc
    38881 ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg
    38941 tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca
    39001 aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct
    39061 aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct
    39121 tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct
    39181 tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct
    39241 tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca
    39301 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
    39361 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
    39421 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
    39481 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
    39541 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
    39601 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
    39661 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
    39721 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
    39781 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
    39841 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
    39901 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
    39961 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
    40021 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
    40081 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
    40141 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
    40201 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
    40261 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
    40321 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
    40381 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
    40441 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
    40501 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
    40561 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
    40621 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
    40681 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
    40741 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
    40801 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
    40861 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
    40921 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
    40981 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat aattcaaact
    41041 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
    41101 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
    41161 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
    41221 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
    41281 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
    41341 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
    41401 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
    41461 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
    41521 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
    41581 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
    41641 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
    41701 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
    41761 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
    41821 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
    41881 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
    41941 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
    42001 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
    42061 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
    42121 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
    42181 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
    42241 cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt catcaacttc
    42301 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
    42361 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
    42421 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
    42481 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
    42541 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
    42601 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
    42661 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
    42721 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
    42781 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
    42841 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
    42901 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
    42961 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
    43021 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt
    43081 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
    43141 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
    43201 atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg
    43261 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
    43321 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
    43381 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
    43441 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
    43501 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
    43561 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
    43621 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
    43681 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
    43741 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
    43801 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
    43861 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
    43921 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
    43981 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
    44041 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
    44101 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
    44161 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
    44221 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
    44281 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
    44341 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
    44401 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
    44461 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
    44521 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
    44581 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
    44641 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
    44701 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
    44761 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
    44821 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
    44881 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
    44941 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
    45001 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
    45061 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
    45121 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
    45181 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
    45241 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
    45301 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
    45361 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
    45421 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
    45481 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
    45541 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
    45601 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
    45661 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
    45721 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
    45781 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
    45841 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
    45901 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
    45961 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
    46021 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
    46081 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
    46141 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
    46201 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
    46261 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
    46321 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
    46381 tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
    46441 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
    46501 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
    46561 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
    46621 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
    46681 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
    46741 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
    46801 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
    46861 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
    46921 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
    46981 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
    47041 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
    47101 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
    47161 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
    47221 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
    47281 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
    47341 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
    47401 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
    47461 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
    47521 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
    47581 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
    47641 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
    47701 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
    47761 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
    47821 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
    47881 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
    47941 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
    48001 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
    48061 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
    48121 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
    48181 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
    48241 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
    48301 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
    48361 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
    48421 gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg
    48481 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
    48541 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
    48601 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
    48661 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
    48721 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
    48781 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
    48841 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
    48901 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
    48961 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
    49021 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
    49081 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
    49141 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
    49201 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
    49261 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
    49321 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
    49381 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
    49441 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
    49501 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
    49561 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
    49621 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
    49681 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
    49741 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
    49801 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
    49861 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
    49921 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
    49981 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
    50041 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
    50101 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
    50161 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
    50221 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
    50281 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
    50341 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
    50401 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
    50461 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
    50521 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
    50581 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
    50641 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
    50701 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
    50761 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
    50821 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
    50881 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
    50941 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
    51001 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
    51061 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
    51121 aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg
    51181 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
    51241 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
    51301 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
    51361 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
    51421 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
    51481 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
    51541 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
    51601 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
    51661 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
    51721 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
    51781 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
    51841 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
    51901 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
    51961 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
    52021 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
    52081 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
    52141 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
    52201 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
    52261 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
    52321 acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac
    52381 atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa
    52441 gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt
    52501 tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga
    52561 ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga
    52621 acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct
    52681 tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct
    52741 gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag
    52801 caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt
    52861 gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa
    52921 agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc
    52981 gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat
    53041 aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt
    53101 tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat
    53161 cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca
    53221 tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac
    53281 agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga
    53341 aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac
    53401 aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc
    53461 tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc
    53521 ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc
    53581 agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag
    53641 acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca
    53701 aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga
    53761 cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag
    53821 aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg
    53881 gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt
    53941 tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc
    54001 aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg
    54061 tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag
    54121 cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac
    54181 cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg
    54241 atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata
    54301 ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg
    54361 cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg
    54421 ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc
    54481 gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt
    54541 tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg
    54601 gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt
    54661 tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt
    54721 tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc
    54781 ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt
    54841 ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat
    54901 tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga
    54961 ttacgctact gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc
    55021 acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc
    55081 ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt
    55141 ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa
    55201 gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt
    55261 tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct
    55321 gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta
    55381 ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta
    55441 attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca
    55501 ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg
    55561 agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt
    55621 tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt
    55681 tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc
    55741 gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt
    55801 tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg
    55861 tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    55921 ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg
    55981 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
    56041 tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc
    56101 ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat
    56161 aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac
    56221 acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga
    56281 agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat
    56341 aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt
    56401 tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg
    56461 ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag
    56521 cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa
    56581 tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac
    56641 tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt
    56701 tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa
    56761 tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg
    56821 gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt
    56881 accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac
    56941 accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc
    57001 agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact
    57061 agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa
    57121 cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc
    57181 tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact
    57241 tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc
    57301 catccctttc tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt
    57361 aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt aggattttta
    57421 tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg
    57481 aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc
    57541 ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa
    57601 aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg
    57661 aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc
    57721 tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca
    57781 tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac
    57841 gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc
    57901 aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa
    57961 tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc
    58021 gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca
    58081 agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca
    58141 ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac
    58201 ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca
    58261 ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc
    58321 atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta
    58381 aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg
    58441 aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa
    58501 ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct
    58561 tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta
    58621 gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac
    58681 ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg
    58741 atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg
    58801 ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt
    58861 cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa
    58921 gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg
    58981 aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac
    59041 tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt
    59101 acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc
    59161 atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata
    59221 gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg
    59281 cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat
    59341 ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc tgatttgttg
    59401 ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat
    59461 cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt
    59521 tttctccaat tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc
    59581 tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact
    59641 aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt
    59701 tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag
    59761 acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc
    59821 cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata
    59881 ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa
    59941 aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat
    60001 ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc
    60061 aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct
    60121 atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa
    60181 aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct
    60241 gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa
    60301 ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa
    60361 tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca
    60421 aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat agtgatcttt
    60481 tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata
    60541 ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg
    60601 aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata
    60661 gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg
    60721 taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat
    60781 tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc
    60841 atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag
    60901 atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat attgacagaa
    60961 gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat
    61021 gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt
    61081 tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa
    61141 tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac
    61201 ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg ctttttctgt
    61261 tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt
    61321 tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg
    61381 caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt
    61441 aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat
    61501 ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga
    61561 ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc
    61621 tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa
    61681 tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca
    61741 aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa tttccgaaat
    61801 cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat
    61861 aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt
    61921 gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg
    61981 ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc
    62041 tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt
    62101 atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc
    62161 accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc
    62221 agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg
    62281 ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag
    62341 ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag
    62401 acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc
    62461 gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca
    62521 agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac
    62581 gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta
    62641 cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga
    62701 agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt
    62761 aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat
    62821 gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc
    62881 aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt
    62941 ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg
    63001 gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc
    63061 ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga
    63121 gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt ctgaagaact
    63181 cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac
    63241 tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct
    63301 cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca
    63361 atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc tgcataaaaa
    63421 gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac
    63481 tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc ctgtgtcgtc
    63541 ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc aaatgctaca
    63601 gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg aagttcaaga
    63661 gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg
    63721 atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg
    63781 acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca
    63841 gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa
    63901 attccattta acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga
    63961 ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga
    64021 ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct
    64081 gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct
    64141 tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct
    64201 tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt
    64261 ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt
    64321 actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg
    64381 gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca
    64441 atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg
    64501 attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg
    64561 aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg
    64621 aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct
    64681 gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct
    64741 agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct
    64801 tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct
    64861 ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact
    64921 ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta ttccttttaa
    64981 ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg
    65041 acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat
    65101 atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca
    65161 cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata tctttattca
    65221 aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca
    65281 atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct
    65341 gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg
    65401 aatgcagatg atatcataga caaactggat agcggattac ttgattttgg actcgtaatc
    65461 gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga
    65521 cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc gcaggaactg
    65581 aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg
    65641 ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct
    65701 tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact
    65761 tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc actgaatctc
    65821 atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg
    65881 gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag
    65941 ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc ttccttttat
    66001 ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt
    66061 ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca
    66121 tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg
    66181 ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga
    66241 aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag
    66301 tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg
    66361 atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta
    66421 atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg
    66481 ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt
    66541 ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag
    66601 tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc
    66661 ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac
    66721 gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca
    66781 tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt
    66841 attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg
    66901 ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga
    66961 tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag
    67021 aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg
    67081 ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct
    67141 tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg
    67201 ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt
    67261 aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc
    67321 aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac
    67381 tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca
    67441 gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg
    67501 tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc
    67561 aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc
    67621 ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc
    67681 aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa
    67741 ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga
    67801 aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg
    67861 gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac
    67921 agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa cagaaccact
    67981 tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg
    68041 gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg
    68101 atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat
    68161 cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa
    68221 attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac
    68281 gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg
    68341 agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga
    68401 tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat
    68461 cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat
    68521 ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg tggttaaaac
    68581 agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa atgggaaact
    68641 gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta
    68701 tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat
    68761 tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca
    68821 tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg taaaagaagg
    68881 tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc
    68941 aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga
    69001 aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc
    69061 ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa
    69121 tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat
    69181 aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc ttattacttg
    69241 atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag tttatcggta
    69301 tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttcatactc
    69361 aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa tatttctttt
    69421 aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc atcagaggaa
    69481 atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc agctgcttct
    69541 tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc ttcaataatt
    69601 tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata gtcagttaga
    69661 ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt tgctgcagct
    69721 tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc ataaacgaat
    69781 tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata ccaattattc
    69841 cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa ttggtgatct
    69901 ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc gatataagat
    69961 accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg atttcctgga
    70021 acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat tgagtctaaa
    70081 tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc tcctttttct
    70141 tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa gaattccagc
    70201 gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt tgccactccc
    70261 tattcttttt atttgttaag atttacagct gtacatttat aaattacacc tttatcataa
    70321 agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga tgaagtattc
    70381 tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc aagatacccg
    70441 atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa
    70501 aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa aaccgcagcc
    70561 ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca gcatcgaagg
    70621 taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa
    70681 tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag aagaagaaat
    70741 ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa aagaaaaaag
    70801 accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt gcctgcattt
    70861 ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa gccttgtcat
    70921 gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt cttgataaac
    70981 ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat
    71041 ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc tctatattat
    71101 catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt ggtaactccc
    71161 atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc tcttaaaaca
    71221 tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc tgctaaagcg
    71281 attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt tggaacatcc
    71341 tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc gttgactgta
    71401 aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt accgataact
    71461 ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt gctactcttc
    71521 aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct atactttgcc
    71581 atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa gggaaatgac
    71641 acgattacgc tcttttttct agggatattt ttatttttta actatttttt tgctttcact
    71701 gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt aaaagagatc
    71761 tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata gattttcccc
    71821 caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc ccaagtgtgt
    71881 tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt agcaaagcca
    71941 atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc ataagaaacg
    72001 ttgattactt gatttggata taaatctcct aacttatgca tctcttccct ttgagtctcc
    72061 gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt gacaggaact
    72121 tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa ttccatcgat
    72181 tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat
    72241 ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga aaaaccttcg
    72301 actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc taggagatcg
    72361 cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat ccctgcaata
    72421 aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc tattatttta
    72481 taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt cagatcttta
    72541 agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa agtcaattac
    72601 ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa
    72661 ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat gttttacctt
    72721 tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa aaatatcttt
    72781 tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg ttcaaactgc
    72841 tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt atcaattgga
    72901 acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa aaacaaaaaa
    72961 agttccatct ttaaaacacg ctattctttt actttttctc acattctcta agaaaagaaa
    73021 aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta aaatcagcta
    73081 cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga aaggagaact
    73141 agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt tacagctatc
    73201 atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc gcaacagttt
    73261 ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac atgacttgtg
    73321 attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt
    73381 acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg tcccttgttt
    73441 cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata gaacatccgc
    73501 aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat tcagataaat
    73561 taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac aggaaaacag
    73621 cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg ctcattcagc
    73681 aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact ctcgaaacat
    73741 tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt tattatttat
    73801 ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg
    73861 tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata aaaatcctga
    73921 aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat aaaaaaacta
    73981 aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc
    74041 ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga
    74101 agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg
    74161 ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca ttgttgatag
    74221 attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt atattggata
    74281 aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag ataaatatta
    74341 aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc tatctttgca
    74401 tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa tgattatcct
    74461 taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg atccttattg
    74521 aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta tctcatagta
    74581 aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg taaaaacggt
    74641 aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc tcgccagtct
    74701 ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat ttgcactact
    74761 acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga aaaaatcggt
    74821 gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc aaaaataata
    74881 ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc cttataatct
    74941 tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc cgatccaccc
    75001 attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg ctctttgttt
    75061 gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa caatgaacct
    75121 gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga tgtaattaac
    75181 aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgtaactat cgtggtaatt
    75241 agcttattta tatccatatt aaacaattga atgattataa atgacaataa agcaaacgag
    75301 gtaatcgtaa taatgattgg gattaataca ttaaaagact ttaaaacagc gggaggaata
    75361 tttccggata gctgaatctg tagtttctta ttattagaaa gtttaataaa taattctgta
    75421 gccgctaatc caacaaatat acctacaaac attcctgaag ctcctgtata ggatataggt
    75481 atcacacccg tcacttcaac agctttatcc gcattttcag gagtaaacat catctgcata
    75541 ggtgttaata caacaaatgt cgaaatagca actaataccg ctgcaatatg atttgtataa
    75601 tttctgcttg cacacatatg ataagatact gctgctacaa ctaataatgt agttatgctt
    75661 aatgtaccat tggcaattga agtaccaatt tgttgtaacg tggtcaatgt actagtatcg
    75721 acaatttccc ccatgaatcc tgttggttct aagatcactg aattaataag tgtcacaaat
    75781 cctgctaaca tcataaatgg cataaatgta gcaaacgcat ctcgcaaagt tcttaaatgg
    75841 atctgattcc ccaacttagt cgcaactata ccaaattttt caaatacaat gtccttcctg
    75901 ctacttttgc ccatattttt tctcctttat gcttattgta atcgattact ttttccaccc
    75961 aaataataca tcacttatca aaaaaagtaa atacttttaa ttaaatatat atatcttttt
    76021 tagtataaaa aaataagctc tgaaaaaata attttcccga gcttattttt caacaaaaac
    76081 agcttcaaaa acaaagtctt gatataaatg tctcgatacc gaatactcga aaggtttacc
    76141 agacgataga gcaacaactt tgtctatctc aatcataaat tcacctggat ctatttttaa
    76201 tatttttgcg tcaaattcat tcgcagtatc tcctttgata cgtatgtgcg aagtctttat
    76261 ttccagaccc agttcatttg tgatataagc atatacagat ttctttaaat gttttggttc
    76321 caagcctggt atgactgcta atggcatgaa tgtttgctca atcgagtatg gttgacaatc
    76381 aataaaacgg cttcgtacaa tattatatac atagtctttt acactgatac ttaatttatc
    76441 agctactttt ttcgatggaa gttcaattga gaaagtatgg atttctgaat ctacgtgttc
    76501 ttcaccaacc acgctaaaca aaccttcgtt tggaccaaga actcgtgagt tatcaattgg
    76561 tgttctgacg acttcagtcc catacccact tctacttcgc aaaactcctt cggaaacaag
    76621 taagtctacg cctttcttta ctgttaactt gcttgtatga taaataactg ccaattcatc
    76681 tcctgtagga agtttctcac caattttata ctctccattt acaatttttt tctttatatc
    76741 taagtaaatt tccatatact tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat
    76801 atcttcatct atactttaac ataaataaac cgataaaatc cacatcaaaa ttatacacac
    76861 aataaaaaag atatatatat ttaaacaaaa ctatttactt taagttgtaa gcggtgttat
    76921 cattaagtgg aaacaggagg taaaaaaatt atgacaacta tgaaaaagct aacttttcca
    76981 gatgattttt ggtggggttc cgcttggtca gctgagcaag cagaaggaaa aggagaaaca
    77041 ggaaaagcag agactatctg ggatcgctgg tttaaagaac aaccttaccg gttttatcaa
    77101 cgaatcgatt cctctattac aaccgatcac atccatcaat ataaagaaga tatagaacta
    77161 atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt cttgggcacg aatgtttcca
    77221 aatggcggta ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa attattttcc
    77281 gaaatgaata ataacggaat caaagtattt gccaacttat atcattttga catgcctgct
    77341 actcttcaag ataaaggagg atgggaatca cgtgaagtag ttgatgctta tgttcattac
    77401 gctgaaactt gttttaaaga atttggtgac ttagtttgtc attggtttac cttcaatgaa
    77461 ccacttgggc ctattttagg tacttatctt gaagattttc attatccaaa cctcattgac
    77521 tttaaacgcg gagcgcaagc agcattcttc actatacttg cacacgcaaa agcaatacaa
    77581 gtatttaaag aactagctct tcctagtaaa ataggcgtta ttctaaattt gagtcctact
    77641 tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga tttattttat
    77701 acgcggagct ttttagatcc tatggttaaa gggaattttt ctaaaaagct tatcctgtta
    77761 ttacaagaat ataatcagct tcctgaagat tatacgaaag aagatcttgt atgtatctac
    77821 gaaaataccg ctcaaattct tggactaaac tattacgagc cacgtcgcgt caaatctcgt
    77881 tcttctatgg tggatacatc tagtccattt ttaccagagt ggtttttcga accacatatc
    77941 atgcctggac gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa aggaatttat
    78001 gatttgtgca tggatatcaa gaaccattat ggaaacatcg aagcatttat ctcggagaac
    78061 ggaatgggcg ttgcggatga agaacgtttc atggatgata cagggcaaat aattgatgat
    78121 tatcggattg agtatatcaa agatcatctt gcttacttat ggaaggcaat aaatgaagga
    78181 tgtaacatta aggggtatca tctttggaca tttattgatt gctggtcatg gatcaacgcc
    78241 tataaaaacc gatatgggct ggtttcactc gaccttacta ctcaaaaaag aacaattaaa
    78301 aaaagtggcg aattttacaa gcaattaagt gatacaaatg gatttagata cgatgtagac
    78361 ttgttagttg aataatattc taaaattcca cttgaagtga acacattaga aaacaagaat
    78421 aatgagaaat accaataaca aatacttttt cttcatactt catttatact tgtcagtata
    78481 agagactgtg acaaatgtag tgatcctcag aagttaggtc tggaaatcta acttgagaag
    78541 atttttttaa actacgtcca gtctttttta tttgtttaaa attgaagttg acaagtcttc
    78601 attaaaaatt tagaatattc acaagtttga cgacaattat gaaaatttat gtcgtgctaa
    78661 aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc
    78721 taccgttttt ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg
    78781 gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt
    78841 tatcacggtc agcatgcttg ctagccatag tccactgctc tccatcgttc tctcgacctt
    78901 tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa
    78961 tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg
    79021 aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa
    79081 ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt
    79141 tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt
    79201 actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat
    79261 tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagta acttagtgat
    79321 tttagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg
    79381 agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct aggatcctac
    79441 catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg
    79501 tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac
    79561 atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta
    79621 ttctttcaaa aagcttattg gtgatcgtct tggtaggaat tctttccctt tcgttgattc
    79681 gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt
    79741 tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact
    79801 aacaacgact tttcctaata tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt
    79861 cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt
    79921 tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc
    79981 tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat
    80041 atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg
    80101 tttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc
    80161 gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac
    80221 acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc
    80281 agcccaatgc gctagttgat tcaaaatcga accgacgccc ccagctccgt tgatcactaa
    80341 gatttttttc ccgcggttgg cattttcttc tggagtcaga ccaaattttt caaataacaa
    80401 ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg aaagattttt
    80461 tggtgcaaga gcgacaatac gttcgtcaac cagttggtat tcttgattgc ttcctgctct
    80521 tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcatcacc
    80581 tacagcaaca actttcccga ctccatcaaa ccctaagata cgtaaagcat ttcttatccc
    80641 gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag
    80701 aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc
    80761 atcgatcgac aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg
    80821 aattcctcct tagcttcatc aagttggagc atccatacag ttttcatttc aatatatata
    80881 gtatgctatt tgttccactt ttattcatca taattattct cttacttaaa gtaaactatt
    80941 tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttttaaac
    81001 ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac gacaaaatat gaggagctat
    81061 ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacgacc tctaattcac
    81121 ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg
    81181 tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac tcgcaagtca
    81241 tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa
    81301 cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag tagtccgatc
    81361 agttcttttt ctgtatgttc cctcggttct ttttcaaatg cataaggatt cttgaaaatc
    81421 ccccggccaa tcatgatgcc atctacgcca tatttttcgg ccaattcttc tcctgtctgg
    81481 cgatctggga tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa
    81541 gcaatgattt tagggatgac ttcccaatgt gcatctactt tactcatctc tttacgcgta
    81601 cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat ccattcttcc
    81661 atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa accgccagct
    81721 ttagccgctt caatcaattg tgccgctact tctgggcgaa ggatcaagcc actgcctttt
    81781 ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc ttggaagccc
    81841 atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc ccaaatatgg
    81901 gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca
    81961 tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc cggtgctgct
    82021 gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg tgctaaaaca
    82081 aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt actctccatt
    82141 ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact aggaaacaaa
    82201 aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa agactgtgac
    82261 aaaaatctta tcacaattcc ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg
    82321 taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    82381 ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct
    82441 cggtattagc tcaacaaacg acaaaatatg aggaactatt ttcccgtttg tttctctaat
    82501 actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta
    82561 ttgatcaaat accaagtaac ggcgtacaaa acaacgatga atagcacaag tacgatcatc
    82621 gatgttgcta ctggcacatc gattgttcca aggaatccgt aacggaaacc ggagatcata
    82681 taaacgatcg gattgacttt cgaaacagct tgccaaaatg gcggcaacat agagatggca
    82741 tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca
    82801 tcgtcataag actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa caagattgcc
    82861 gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac
    82921 aaggaaatga tcgttacgag agagccgacg agaatactgc gacccactcc gccaatcaca
    82981 aagccccaaa tgatcacatg cgtaggaaca ggcgctacca agatttcttc gatgtttttc
    83041 tggaattttt gagagaagaa ggacgaagaa acgttggcat acgaactagt gatagcagac
    83101 atcataatca aaccaggcac gatgaattcc atatatgaaa aaccacccat gtcaccgatc
    83161 cgaccaccga tcatttttcc gaaaataaca aagtacaagg atgtcgtgat cacaggcgga
    83221 accagtgttt ggacccagat acgcaaatag cggttcgttt ccttgatagc taaacttttt
    83281 aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat
    83341 tttcaaaaac agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc
    83401 ttgtcttgtc agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc
    83461 gattgtctgt tcatcttcaa atgtacaagc gtatccttca atcacaggtt tggtctcata
    83521 tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg gatagaagct ctttcatact
    83581 agtattctcg atcagttcac ctgattggat gatcccgatg ttgcgacaaa gcatttcagc
    83641 ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcggag
    83701 aaactcccac atctcgcgtc tcaattcaat atcgactcct gctgttggtt catccaagat
    83761 cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc
    83821 ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt
    83881 ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac
    83941 gatttgctgg accgtttcaa agggattgaa attgaactct tgcgggacta atccaatctg
    84001 ttgctttgct cggactagat ccgtatccag atcataaccg aagacattca cttttccaga
    84061 tgttttattg actaaagaag taatgattcc gatcgttgta gacttcccag ctccatttgg
    84121 tcctaatagc gcatagaaat ctccttcttt cacggaaaga tcgattcctc gcaatgcttc
    84181 gaccccactt ccataaacct ttttcaaatt atttatttca agcgcatttg ccatttattt
    84241 cctatctcct tatagacatg attctttatt gtatcatgga atacagcaga tgaaaaaaca
    84301 acttggttcc agcgaaaaat caggttgctg ggctcaggaa aatctcctaa tgaaagacat
    84361 caatcggacg aagtcagaca accttttcag aaaaaacaaa tcgaagataa aggattacta
    84421 ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag aacacttttg tctcacgctc
    84481 taaacacgta taaacggcgg aagcaaaagc caagattttt gatccaataa atactttcga
    84541 tcacttcgcc tatcccaata catgccactg ccgcttttca tcaaataacg cggcttccgt
    84601 ttttgcggtt tctactaaag tctctgggta atccaaaact ttcagcaagg caatcgcatt
    84661 tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt
    84721 tacttgttct tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat
    84781 atcatgtgtg gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc
    84841 ggaagaagca atccgttcga tcgtatttgt cccttttaaa atctcatcta cgaagcagta
    84901 gcagcgttct ttggaagcca cttggtccaa caaacgtttg atcgatttga tttctgaaac
    84961 aaaataactg tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc
    85021 aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct
    85081 gatggctata cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt
    85141 attcttctgc cagttgaccg gattcactac cgcttctttt aacaatggat gatagatatc
    85201 tttcgctgtc acaccgccct cagtaaactc tggtcggcaa gtgatgggca tataagtacg
    85261 gtaattcaat attgcagcgg ctacttctaa ttctcccagc aattcccaca gcttgatggc
    85321 ttcagttgaa tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat
    85381 gaacatgatg ttgacatatt caaaaatcaa ctctgcttca ctcccactct tcatacgaaa
    85441 tgaaaaacct aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctcttcttg
    85501 aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa ccaaataact
    85561 catgctattc agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa
    85621 tactaaccca ccgattgcca aatagattcc tagtacttgt cctaaaagaa gcaaacccaa
    85681 acccaccagc ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact
    85741 ttttccatcc gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa
    85801 ttgatattgg attttttctc tcagttgagg attttcctga taaaaagcaa tcagtttttc
    85861 taactgctga tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc
    85921 tacactagaa taggtactgt tgattccttc gaatacttcg aacatatcca ggtcatacca
    85981 agtaagatca tcgatttcgc tatcccatga acggaacttc ttctccgtga gccatgcttc
    86041 cttcaagctt tcttctttat caaatctcgg ttgataagga attttttccc atttgctacg
    86101 aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa gcaactaata aaataacagc
    86161 tgcaataata attaaaacga taaattctct ttgacccata tccctctccg ttcctttatt
    86221 tgttagcttt agtatagcac tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca
    86281 aaataaaagg tgtttttttc ttgtatatta catatttatc tctcatttat caaaattgac
    86341 aaataatcat agggaggaat tgttgttaat tcttgtttat tctccagtgc attgacaact
    86401 gtcccaaggg aaaggatagc ctcaggtact attttttctt gttctgttgg attagctaag
    86461 cgaagaacaa atccttgttc atgaaaataa gaaggataaa tcgatgatac aattaagtct
    86521 tcaggtaatt caatcaatga aaatttacga ggaacatcaa gttcattttg taatggccaa
    86581 atcttgttat ctaacctgtg aataaataca ttcagtttct gcttttgata agaaatagat
    86641 tgttctaacc gtttttctgc ctgttttgcc actttgaact cattaaatct tccttcttct
    86701 acattaaatc caaaagtgac tttatactca cctatctctt gagctagcgg tgtttccatc
    86761 atgacatgcc cttcatttgt cgtatctcct gaagctcgcc caggtcgcca agcaagatta
    86821 ggttttccaa gttcccctgt tgttgaaaat aaggttatga ttaattgatt ttctatacgt
    86881 tcatactctt ttaaaccatc tgcataaaac gtaaaataat gatcattttc tgccacagag
    86941 acactcttat cgaatatttc taaattaaca ggtttctcta ccatagtttc tgaccaattc
    87001 ggaggaatga catcaggggt attttcaata aatccgtttt gaatttgtgc aattgatttt
    87061 tcagatttat ctaaaacatt aattgctaag cgcatacgat gcgagtaaac agtattatga
    87121 attttgagtg tgccttcaac gatttgactt tctcccagac tgaacgatat ttcataagat
    87181 atttcttcca tttcgggttt ttcagataaa cgatctttta aatctttagg aagttgagct
    87241 tttccgtaaa caactaatgt ttcttgtaaa gaatctttat atactttagc tgtttctaat
    87301 tttagagata gttctgtatc tccttctaat ggtgaataat catacgtatc gccatcattt
    87361 gctgaatcga tcaaatgaac gaaatcaaga tattgtctat ctccaactat caaattcaat
    87421 tgcccatctt taaaagaaag tttatattta ttgttactta tctcagtttt gtctatctcg
    87481 attgctgttt ctaatttttc attatttttc tcaaattcaa caacagtgta tcctagtgct
    87541 ggcacattta catttatttt tatgtcaagt tcataataag ctggttcatc aaaatattcg
    87601 aaacctgtag caatcatccg ataagcattt ttccgttctg gaaaataaac ttctttttca
    87661 atgatattat agggactacc tttgaagtgt attttctttg aatcagctac tacatgcacg
    87721 attttcgatc cactataatt ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt
    87781 agctttaatg catcagccat ccgttttaca ataaggtttt cgattccgtc agctatctca
    87841 ttagcttctt tgattcgatg gaaaatatct tccgcaacat tatctgaaac acagccacca
    87901 atactatcat gagcctggtt ttgcaaaatc tttttccata accgacgaag taggccttta
    87961 ctgatatgga taccatttct ttctgcaatc agcatgagcg gctcaactcg ttttagaatt
    88021 ttttgttcca atttaaaatt ttctattttc atttttgttc ttactgagcc aatagaacga
    88081 tgaacacgag agtaaaccgg ctgacgaagt tcacctttat attcaggtaa ttcttttttg
    88141 tttctgataa cttctacata agaaggataa tctgaaataa tattgtggaa tttactgtat
    88201 ttattcaact tctctacgac ttgatcaaaa tttaaaatca ttgacttttg atctacaccc
    88261 gaaggtacta aaatatcttt gttttttcct tgcatatgaa gcatttcaac tgcttcatcc
    88321 aacttttctt ttgcaaattc aggaatatct ttaattgcgt ctaaggtcat cattccagtc
    88381 atataaccaa atggaaaatt catggcataa acaaatttat ctcccaatcc tttccagata
    88441 aaataaggag atggaaccat cgtttcaaag tttaatcctc gccaacacat gaaattatcg
    88501 atttctactt gatgcaaaag tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa
    88561 taccctacca tcattggatc tccatattta aggatcgtat cactgattcc aatcattaag
    88621 tttcttaaaa ttgattcagc atccactaat aaggcatctg tttgtgcata ccacggtccc
    88681 acgaatagtt gtcctgtttt catcagtcgt tggataacct taagcttttg cggattgatc
    88741 tccaaatact catcgacgat tgaagtttgt ccatctaaac aaaagtttac tttagggttc
    88801 ctttccaatt catctagaac ttcagtaaag acctgatcag acaatacgat ggcatcttca
    88861 tttgtaaaat accattctct gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata
    88921 tcagattctc ctactttcct tcttctactg aatttacttg ttgttctttt ttatacttga
    88981 tggattttag tgcattgacc attaacgctg taaccactcc cccaataaca attgatacta
    89041 aatataaagg tatattcgta acaaaaggaa ttgccataaa ggttgaaagc attgtttctt
    89101 gagtaacacc aaaaaatcca tttagaccac atgctactgc agtgcccacc acgatgcttg
    89161 gtattactcg taaaggatct ttcacggcaa acgggatcgt cccttcagta atccccacaa
    89221 ggcccataat catagctgtc tttgcattat ctcgttccga ttcatcataa agttttttgt
    89281 ttaaaattgt tgctaaaaac attccaaatg gcggaatagc aattcctaat aacatggctg
    89341 tactaggtcc aaagcttccg cttgaaaaag aagcaacccc aaaagcatat gcgattttat
    89401 tgataggtcc tcccatatca aacgccatca ttcctcctaa aatagccgac atcaaagcag
    89461 catttgctcc tgacatgcct tctagccaat tagtcagtcc tgttgtcatt gcttgaatag
    89521 gcgtagctag tacataccat aatatgattc caacaactaa tgtagtaaca aaaggaatca
    89581 gcataattga ttttagacta ataacttgca tcggtacttt tacttttttc aatgcattag
    89641 ctaaatagcc acaagctaat ccagcaacca ttcctccaat aaatcccata ccaagatttt
    89701 gacacatcat cccagtgata aaagctggtg ctagaccggg acggtcagca attgaatatg
    89761 cgatataggc tgaaatcatc ggtacgataa actgtaatcc cgtctgacca atcattcgca
    89821 ggactcccca gaatgtatcc gtctggtccc aaacacctgt gccaccaaaa atgactgcaa
    89881 tagaagttag taaccctgct gctacgacaa gcgggatcat ataggaaacc ccactttgga
    89941 aatgatttag aatttctctt ccttgtttct tgaattccat tagtaactcc cccacgatat
    90001 attttttatt tgttaattaa tgcaattgct tcatcaatcg tctcttcacc atcttttact
    90061 acctctgagg tccctactgt taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca
    90121 tttgtatcta ctgcaaatat gacaacatct gcctcggaaa catccgaaat tgaaagtttg
    90181 ttttctatcc ccatagcccc ctgtgtttct actttaatat ctaacccttt cttcttcgca
    90241 aacttttcta aattagcttt tgccatataa gtatgggcaa tccctgcgat acatgctgtt
    90301 actgctacta tttttgtcat atgaaattcc tccttttaaa atgatagagc ttttaccaat
    90361 tcttttttat ttctcgaatc ttttattctt ttaacaaact cgtcatccat caatcttcca
    90421 gataattctg ctaaaatacg taaatgatta gtacctttat cagtgtcttt tattgctaac
    90481 aaaaagacga gatcaactgg ttgatcatct aatgcgcccc attcaacaga ccgctttaac
    90541 ttggctacag ccactgttga ttcaattact gctaggcttt tgccatgagg aatggcgata
    90601 tttttcccaa ttcctgtggt catatggttt tctctgtcaa atacgtcttt ttgaaattgt
    90661 ttgtggtcag acacaaaccc attagtctct aacaatttcg ataatttttc gactacactt
    90721 tttttatcag atgcatttaa atctatcact atattattta tattgatttt ttcactgatc
    90781 atttataagc cttctttcaa tatataaact aatttttcaa acgacgaact cgtttttaaa
    90841 tttgctaata actcatgatt cgaagcgatt tcattgaatg tttcataaat tttattcaat
    90901 tcaggtttta ctttttcatt aagtgcaaaa aagaaaacag aatgaacaag tgtcccatcc
    90961 caatcaattc cttctggatt aacgtagaca cagattactg gcttttttat aaaatctact
    91021 tcgatatgcg gcatagcaaa atcattcatc gctgttgaag atagtttttc tcttttgatt
    91081 gctccctcta gtatcccttc tttcgcgtaa ttttctttta ccaatttttt ggtaattata
    91141 tttaaaacgt tcacttgatt atcctttatt tggtgggtaa aaaagcaaaa tcgtttatct
    91201 aataaattta gaaaagcatc ttctgtttgt tcatttaact gtagatactg ttttactttt
    91261 ctgacatctg cccctaacat caaaggacta acaacaataa caggaaaatt tgtattttca
    91321 atcggaatcg tgcttataac aagttcatca gtgacttttc tatcttgtag ttcattgatg
    91381 cttagtacat cttctatctc aaccatatct gcaaaaccgt tatttattct ttgctctaat
    91441 aattttgatg ttccatatcc gctactacaa actaatatca ctttcgtttt gtcaggtttg
    91501 taacgatcca aaagtgattg aatatggagt gttacgtaag cgatttcatc ttcattaaaa
    91561 tgtatacaga atctctcttc gatttcctct aataaatcta cacttatcat aaatgacctt
    91621 ttaaaagaat atttgatttt gtccgtataa gggttataga tatttactcc caatttgagt
    91681 cttttaactg ctgcattgag atgtaatact agattattga tcaattcttc atcaggataa
    91741 aattcattta atttaattct tactaactct tgtagctcaa cacccatatc taaataggaa
    91801 agcttcgttt gatctaagtc gtgattcata gcattttttt caatcgaagt aatgtgaatg
    91861 tcaacataag cttgttcatc tattggcac
//
LOCUS       DAYYCM010000003        91545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000003 DAYYCM010000000
VERSION     DAYYCM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.003"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1689)
                     /locus_tag="ACHAY1_000182"
     CDS             complement(76..1689)
                     /locus_tag="ACHAY1_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8592216.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1830..2738)
                     /locus_tag="ACHAY1_000183"
     CDS             complement(1830..2738)
                     /locus_tag="ACHAY1_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8592217.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            2921..4045
                     /locus_tag="ACHAY1_000184"
     CDS             2921..4045
                     /locus_tag="ACHAY1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8592218.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4272..5189
                     /locus_tag="ACHAY1_000185"
     CDS             4272..5189
                     /locus_tag="ACHAY1_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592219.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5182..6015
                     /locus_tag="ACHAY1_000186"
     CDS             5182..6015
                     /locus_tag="ACHAY1_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592220.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6098..7216
                     /locus_tag="ACHAY1_000187"
     CDS             6098..7216
                     /locus_tag="ACHAY1_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8592221.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7237..9108
                     /locus_tag="ACHAY1_000188"
     CDS             7237..9108
                     /locus_tag="ACHAY1_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8592222.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9102..10325
                     /locus_tag="ACHAY1_000189"
     CDS             9102..10325
                     /locus_tag="ACHAY1_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8592223.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10411..12345)
                     /locus_tag="ACHAY1_000190"
     CDS             complement(10411..12345)
                     /locus_tag="ACHAY1_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8592224.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            12571..13227
                     /locus_tag="ACHAY1_000191"
     CDS             12571..13227
                     /locus_tag="ACHAY1_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8592225.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13301..13654)
                     /locus_tag="ACHAY1_000192"
     CDS             complement(13301..13654)
                     /locus_tag="ACHAY1_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8592226.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            13846..14775
                     /locus_tag="ACHAY1_000193"
     CDS             13846..14775
                     /locus_tag="ACHAY1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8592227.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14786..15622
                     /locus_tag="ACHAY1_000194"
     CDS             14786..15622
                     /locus_tag="ACHAY1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8592228.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            15870..16646
                     /locus_tag="ACHAY1_000195"
     CDS             15870..16646
                     /locus_tag="ACHAY1_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8592229.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            16822..17565
                     /locus_tag="ACHAY1_000196"
     CDS             16822..17565
                     /locus_tag="ACHAY1_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592230.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            17558..19138
                     /locus_tag="ACHAY1_000197"
     CDS             17558..19138
                     /locus_tag="ACHAY1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8592231.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19389..19868)
                     /locus_tag="ACHAY1_000198"
     CDS             complement(19389..19868)
                     /locus_tag="ACHAY1_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8592232.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19884..20636)
                     /locus_tag="ACHAY1_000199"
     CDS             complement(19884..20636)
                     /locus_tag="ACHAY1_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8592233.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            20753..20823
                     /locus_tag="ACHAY1_000200"
     tRNA            20753..20823
                     /locus_tag="ACHAY1_000200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20785..20787,aa:Gly,seq:ccc)
     gene            21184..21909
                     /locus_tag="ACHAY1_000201"
     CDS             21184..21909
                     /locus_tag="ACHAY1_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8592234.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22164..22430
                     /locus_tag="ACHAY1_000202"
     CDS             22164..22430
                     /locus_tag="ACHAY1_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8592235.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22427..22858
                     /gene="ruvX"
                     /locus_tag="ACHAY1_000203"
     CDS             22427..22858
                     /gene="ruvX"
                     /locus_tag="ACHAY1_000203"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8592236.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            22883..23188
                     /locus_tag="ACHAY1_000204"
     CDS             22883..23188
                     /locus_tag="ACHAY1_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8592237.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23269..24414)
                     /locus_tag="ACHAY1_000205"
     CDS             complement(23269..24414)
                     /locus_tag="ACHAY1_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8592238.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24475..25122)
                     /locus_tag="ACHAY1_000206"
     CDS             complement(24475..25122)
                     /locus_tag="ACHAY1_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8592239.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25316..25609
                     /locus_tag="ACHAY1_000207"
     CDS             25316..25609
                     /locus_tag="ACHAY1_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592240.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            25653..25964
                     /locus_tag="ACHAY1_000208"
     CDS             25653..25964
                     /locus_tag="ACHAY1_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592241.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            25961..26585
                     /locus_tag="ACHAY1_000209"
                     /pseudo
     CDS             25961..26585
                     /locus_tag="ACHAY1_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            26726..26884
                     /locus_tag="ACHAY1_000210"
     CDS             26726..26884
                     /locus_tag="ACHAY1_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592242.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            26885..27247
                     /locus_tag="ACHAY1_000211"
     CDS             26885..27247
                     /locus_tag="ACHAY1_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592243.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27284..28132
                     /locus_tag="ACHAY1_000212"
     CDS             27284..28132
                     /locus_tag="ACHAY1_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8592244.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28122..29597
                     /locus_tag="ACHAY1_000213"
     CDS             28122..29597
                     /locus_tag="ACHAY1_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8592245.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            29863..30270
                     /locus_tag="ACHAY1_000214"
     CDS             29863..30270
                     /locus_tag="ACHAY1_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8592246.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30273..30488
                     /locus_tag="ACHAY1_000215"
     CDS             30273..30488
                     /locus_tag="ACHAY1_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592247.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30492..30872
                     /locus_tag="ACHAY1_000216"
     CDS             30492..30872
                     /locus_tag="ACHAY1_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8592248.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31006..31161
                     /locus_tag="ACHAY1_000217"
     CDS             31006..31161
                     /locus_tag="ACHAY1_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8592249.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31230..31703
                     /locus_tag="ACHAY1_000218"
     CDS             31230..31703
                     /locus_tag="ACHAY1_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8592250.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            31700..33394
                     /locus_tag="ACHAY1_000219"
     CDS             31700..33394
                     /locus_tag="ACHAY1_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8592251.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33360..33545
                     /locus_tag="ACHAY1_000220"
     CDS             33360..33545
                     /locus_tag="ACHAY1_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592252.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            33549..34724
                     /locus_tag="ACHAY1_000221"
     CDS             33549..34724
                     /locus_tag="ACHAY1_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8592253.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            34717..36240
                     /locus_tag="ACHAY1_000222"
     CDS             34717..36240
                     /locus_tag="ACHAY1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8592254.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36296..36580
                     /locus_tag="ACHAY1_000223"
     CDS             36296..36580
                     /locus_tag="ACHAY1_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8592255.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            36567..36902
                     /locus_tag="ACHAY1_000224"
     CDS             36567..36902
                     /locus_tag="ACHAY1_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8592256.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37197..37823
                     /locus_tag="ACHAY1_000225"
     CDS             37197..37823
                     /locus_tag="ACHAY1_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592257.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            38565..39401
                     /locus_tag="ACHAY1_000226"
     CDS             38565..39401
                     /locus_tag="ACHAY1_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8592258.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39465..39896
                     /locus_tag="ACHAY1_000227"
     CDS             39465..39896
                     /locus_tag="ACHAY1_000227"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592259.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(39958..41280)
                     /locus_tag="ACHAY1_000228"
     CDS             complement(39958..41280)
                     /locus_tag="ACHAY1_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8592260.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVIYKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41434..43074)
                     /locus_tag="ACHAY1_000229"
     CDS             complement(41434..43074)
                     /locus_tag="ACHAY1_000229"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8592261.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGARTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43407..43877
                     /locus_tag="ACHAY1_000230"
     CDS             43407..43877
                     /locus_tag="ACHAY1_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8592262.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44099..45202
                     /locus_tag="ACHAY1_000231"
     CDS             44099..45202
                     /locus_tag="ACHAY1_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8592263.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45486..46166
                     /locus_tag="ACHAY1_000232"
     CDS             45486..46166
                     /locus_tag="ACHAY1_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8592264.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46256..47290
                     /locus_tag="ACHAY1_000233"
     CDS             46256..47290
                     /locus_tag="ACHAY1_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8592265.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47304..47639
                     /locus_tag="ACHAY1_000234"
     CDS             47304..47639
                     /locus_tag="ACHAY1_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592266.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            47623..50103
                     /locus_tag="ACHAY1_000235"
     CDS             47623..50103
                     /locus_tag="ACHAY1_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592267.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50159..51718
                     /locus_tag="ACHAY1_000236"
     CDS             50159..51718
                     /locus_tag="ACHAY1_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592268.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            51715..53079
                     /locus_tag="ACHAY1_000237"
     CDS             51715..53079
                     /locus_tag="ACHAY1_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8592269.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53250..54320
                     /locus_tag="ACHAY1_000238"
     CDS             53250..54320
                     /locus_tag="ACHAY1_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592270.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54335..55003
                     /locus_tag="ACHAY1_000239"
     CDS             54335..55003
                     /locus_tag="ACHAY1_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592271.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55023..55601
                     /locus_tag="ACHAY1_000240"
     CDS             55023..55601
                     /locus_tag="ACHAY1_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8592272.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56002..56913
                     /locus_tag="ACHAY1_000241"
     CDS             56002..56913
                     /locus_tag="ACHAY1_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8592273.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57291..58208)
                     /gene="rnhC"
                     /locus_tag="ACHAY1_000242"
     CDS             complement(57291..58208)
                     /gene="rnhC"
                     /locus_tag="ACHAY1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8592274.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58410..58847
                     /gene="zapA"
                     /locus_tag="ACHAY1_000243"
     CDS             58410..58847
                     /gene="zapA"
                     /locus_tag="ACHAY1_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8592275.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            58905..59453
                     /locus_tag="ACHAY1_000244"
     CDS             58905..59453
                     /locus_tag="ACHAY1_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8592276.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            59568..61928
                     /locus_tag="ACHAY1_000245"
     CDS             59568..61928
                     /locus_tag="ACHAY1_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8592277.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62015..62329
                     /gene="trxA"
                     /locus_tag="ACHAY1_000246"
     CDS             62015..62329
                     /gene="trxA"
                     /locus_tag="ACHAY1_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8592278.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62511..64289
                     /gene="uvrC"
                     /locus_tag="ACHAY1_000247"
     CDS             62511..64289
                     /gene="uvrC"
                     /locus_tag="ACHAY1_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8592279.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64434..65333
                     /locus_tag="ACHAY1_000248"
     CDS             64434..65333
                     /locus_tag="ACHAY1_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592280.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65326..66588
                     /locus_tag="ACHAY1_000249"
     CDS             65326..66588
                     /locus_tag="ACHAY1_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592281.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            66749..68647
                     /locus_tag="ACHAY1_000250"
     CDS             66749..68647
                     /locus_tag="ACHAY1_000250"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8592282.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(68977..69696)
                     /locus_tag="ACHAY1_000251"
     CDS             complement(68977..69696)
                     /locus_tag="ACHAY1_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8592283.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    69886..69933
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70033..70365
                     /locus_tag="ACHAY1_000252"
     CDS             70033..70365
                     /locus_tag="ACHAY1_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592284.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70352..72355
                     /locus_tag="ACHAY1_000253"
     CDS             70352..72355
                     /locus_tag="ACHAY1_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8592285.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72374..72844
                     /locus_tag="ACHAY1_000254"
     CDS             72374..72844
                     /locus_tag="ACHAY1_000254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8592286.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            72865..73449
                     /locus_tag="ACHAY1_000255"
     CDS             72865..73449
                     /locus_tag="ACHAY1_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8592287.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73464..74450
                     /locus_tag="ACHAY1_000256"
     CDS             73464..74450
                     /locus_tag="ACHAY1_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8592288.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74440..74751
                     /locus_tag="ACHAY1_000257"
     CDS             74440..74751
                     /locus_tag="ACHAY1_000257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8592289.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            74773..76554
                     /locus_tag="ACHAY1_000258"
     CDS             74773..76554
                     /locus_tag="ACHAY1_000258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8592290.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76547..77923
                     /locus_tag="ACHAY1_000259"
     CDS             76547..77923
                     /locus_tag="ACHAY1_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8592291.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            77930..78565
                     /locus_tag="ACHAY1_000260"
     CDS             77930..78565
                     /locus_tag="ACHAY1_000260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8592292.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78595..79950
                     /locus_tag="ACHAY1_000261"
     CDS             78595..79950
                     /locus_tag="ACHAY1_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8592293.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80142..81536)
                     /locus_tag="ACHAY1_000262"
     CDS             complement(80142..81536)
                     /locus_tag="ACHAY1_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8592294.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81628..82599
                     /locus_tag="ACHAY1_000263"
     CDS             81628..82599
                     /locus_tag="ACHAY1_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8592295.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82596..83378
                     /locus_tag="ACHAY1_000264"
     CDS             82596..83378
                     /locus_tag="ACHAY1_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8592296.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83547..83918)
                     /locus_tag="ACHAY1_000265"
     CDS             complement(83547..83918)
                     /locus_tag="ACHAY1_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8592297.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84052..84495
                     /locus_tag="ACHAY1_000266"
     CDS             84052..84495
                     /locus_tag="ACHAY1_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8592298.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84538..85347)
                     /gene="yidA"
                     /locus_tag="ACHAY1_000267"
     CDS             complement(84538..85347)
                     /gene="yidA"
                     /locus_tag="ACHAY1_000267"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8592299.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85366..86733)
                     /locus_tag="ACHAY1_000268"
     CDS             complement(85366..86733)
                     /locus_tag="ACHAY1_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8592300.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86717..87547)
                     /locus_tag="ACHAY1_000269"
     CDS             complement(86717..87547)
                     /locus_tag="ACHAY1_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8592301.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87710..88147
                     /locus_tag="ACHAY1_000270"
     CDS             87710..88147
                     /locus_tag="ACHAY1_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8592302.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88223..88843
                     /gene="rpoE"
                     /locus_tag="ACHAY1_000271"
     CDS             88223..88843
                     /gene="rpoE"
                     /locus_tag="ACHAY1_000271"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8592303.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89032..89538
                     /locus_tag="ACHAY1_000272"
     CDS             89032..89538
                     /locus_tag="ACHAY1_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8592304.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            89555..90454
                     /locus_tag="ACHAY1_000273"
     CDS             89555..90454
                     /locus_tag="ACHAY1_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8592305.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            90541..91425
                     /locus_tag="ACHAY1_000274"
     CDS             90541..91425
                     /locus_tag="ACHAY1_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8592306.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        30212 a        15537 c        18866 g        26930 t
ORIGIN      
        1 cctatatact ttttcatttt tactcctcaa gtaaaaataa ctcctagacc gcggctaccc
       61 accgcggtcg tttttttatt cttcaatcaa aatcgctttg atacccaaac ctgccaagat
      121 tcgggcagaa gtataagtat tcaatcctct tctgaaagta atgtaaactt ttttatcttt
      181 tgggatttca ttgattcgat ctcgtaactc attcattgga atattttttg ttgccgagat
      241 actgccagaa ggtgctttat taggttcacg tatatctaaa aagaacgctt ctttcaaatc
      301 gttttcgggt atatcacttg ctttgatcgt ttctacgata ttcgtcattt gattgattgc
      361 gacataacca gctatattca acgggtcacg ggtagtagaa tacggtggag agtaaggcaa
      421 ctccaaatca ggcaaatcaa agactgtcaa gtttccggtg attgctacag aaagttctcc
      481 catccgttta tcgacacctt tttctccaaa tgcttgtcca cctaaaatac gaccgctatc
      541 tgcatcaaaa atcagcttta gatttaatct tgtagcttct ggatagaaat aggcatggtc
      601 aaagggagtg ataaaaacag ttttataatt tgttatgcct gatgctttta attcatgttc
      661 tgtcattccc gcataacttg ctgtatgatc aaagattttt gcgacacctg caccaatata
      721 tccgcgatag cgcataggcg taccatttaa aatatctgcc agcatatgtc cttgacgatt
      781 ggccgcactg gaaagcatac tttggatcgg ctgaccagtc actacactag tcgtttcgat
      841 aatatcgcca atcgcataga tgtccggcag attggtttgt aactggtcat caacaatgat
      901 ctgacctgta tctgataatt gtatgccggt tgctttcacc acttcattct tcggagaaac
      961 accaacagca aaaattagca tatcagcaga aaggacggag ccatctgata atactatttc
     1021 tcgaccttta tcacggatct cagtaactct tgtttctaaa tgaacggcta atccttcttt
     1081 cagcaatttg tcataaacta aattagcaat ttctggatca taagggaaag cgacttgtgg
     1141 caattgatcg attaaagtca cgtctaagcc gcgatgcttg aaattctcag ctaattccag
     1201 acccataaca cctgcaccaa gaatgacgac atgttgtgga tttttttctt caaggaacga
     1261 tttgattcga tccgcatccg tcacactacg gaggacaaag ccgttttctg cttcaaaaac
     1321 tccgggaata tccggataat ctggtctagc accagaagag atgatcaact tatcgtaatc
     1381 tgtttttatt tcttcatttg tggataggtc tttaactgtt aatcgcttag tagaaggatc
     1441 gattgctgtt acttcgtgtt tagtaaagac atcgatgttg ttttttgttt tcaatatttc
     1501 tggtgtacgt tcgatcagcg agtcacggtc agtgatcaca cctcctaaat aataaggcaa
     1561 agcacaactt gcataagaaa tattttctcc gcgttcatag ataatgattt cgtgcgcttc
     1621 attcaatcga cggaaacgag tggcaaatga aggaccgcca gctacaccgc caatgacaac
     1681 aactttcata aaaacatctc cttttctacg ttgatcgtac tatgttatgg taaaaaacag
     1741 caaataattg tgccggaaaa ttattgttta ttttgaatag acagaaaata tagagaaata
     1801 atttgtggat aaaacctaaa aatttttttc tattttaagg atagataata ctttgaaggg
     1861 gataaccctg tatatttttt aaaagctgaa gaaaaatgat ggatcgaatg aaatcctaat
     1921 atatctgcaa tttctgtata ggttaattca ttttctagca cgagacgttt tgctttttcc
     1981 atttttagtt ctttaaaata gctaataaca ctttttccat tcacttttct aaaatgtttt
     2041 tttaagctgg ctggactgat aaaaaaaatt tgggctaatt gttcaagcaa aaaattttct
     2101 gaaagatgtt cttctaaata tcgtatgatt ggatcaacta attcatctga ataaagaata
     2161 tgcttttttt taggaatgac tttctcattt gaaggtattg tcaataaagc aagttcctgt
     2221 tgataatgcc ttggtaagga agacaattga attttttgca gacaataagt aactaataag
     2281 gaaagcaaat gaatgctcat ctgttggcat agtatgaaaa aagaattgat ttcctgatca
     2341 taggaaatag ctggcttact atgttttaga taatcaattt tgaactgttc atttttcaga
     2401 aaagtaatga tagatgttat ttcttgatct atagaaaaag attgtcccgt aatatgtgct
     2461 aataattgct catcaaaata aaactggata atgataaaag tttgtgtttc cttttgagta
     2521 gaaaagattt ggacttcttg ctgtttatca atgaataaaa gggaaggatt atctagttct
     2581 atagatttat gtgcaaattc agcatataaa ttagcgcttt catttattag tagtcgaaaa
     2641 tagggagcag cagataaatg aagatgggta tctatggaat aggttagtgt ttttttgaca
     2701 agattggagg attggttgtt tgttaacaaa agaaccactt tcctttctct ttttatataa
     2761 aaagctttct atttataatt aatgactatt ataacaaaaa tgaaaagcca aaacgacaag
     2821 taaaatattt ttttatataa atatttgtgt tactaattcc gttaatatac ataagtgaaa
     2881 gcgttttata taagaacttg aaaagaaaga agggatagac atgaaaaaaa gatacaaatt
     2941 ggtgatagct ttgctaaaca ttaccctatt attcagctta agtgcatgtg gaaaaactaa
     3001 cgaagaagta acagcaaaca tcgcagatcc aatgataaaa aaagccacta cattagaagt
     3061 atatgtcgca aacggcaaac tgacaaacta tgttgaaaat gctgcaaaaa tctataaccg
     3121 caaaactgga gcaaatattg acttaaaaat tgtgaatgta gcttcgggaa cagcaacagt
     3181 acaaatgatc accccaaaat tggtaacaca cgaagatatg ccggatatta tctccatggc
     3241 agacacttca gcagcaggtg tgctggaaaa atttcaagat gctttttata gtgctgatga
     3301 atatggattt tatgacaaat atggcgatca gttttatgat caaaaattga atattttgcg
     3361 tcaacaaacg agtaatgaaa cgattattcc ttgggctagt gattttaccc cagcactttg
     3421 ttattatcaa ccaaaaaaat ttgctgaaat aggtgtagat tttgaagaaa tcagttcttg
     3481 ggaagaatat atccaggtgg caaaagcatt aaaagaaaaa actggtagtt ttggaattgc
     3541 cttgccagaa tctggggacc aagaattgtt tatcaacatg ctagcccagc agaatcaatc
     3601 tttgcttgat gaagaaggaa atatccgtct gaatactaaa gaagcgaaac atgctgcaaa
     3661 gttaatcaaa gaaatgatcg actcagatat tgtacatttc tacggtgcac aagatgctga
     3721 gaaagcattt caagaatctg caatgtttgt tgctggcggc tggtatgcta cgaatatgtc
     3781 tttgaatttt cctgatgcat ctgacaaatg gcgcatggct ccactgattc ctttttcaaa
     3841 agaaaatcca ggaaaaggtg cagtatcagg aggatcaagt ttttatgtcc ctaaagatgc
     3901 aaaaaatcca caagtggcac aacaatttct tacatttatg ctgacagatg aagaatgctt
     3961 agccaacgca ctggagctgg gcgtggctac atctaactca ttggcctatc aaacagaagc
     4021 agctgagaaa aagtttgact actaaggagg acaaaaatac tatgaactat tgaataaaat
     4081 caatgaaaat acatcaaata ctgttttacc agccacttat tcagatgcta cgtcttattt
     4141 gactaccgct gcatataact attggcaaac aggtgatttt gaaaattcat atctaaaaga
     4201 agcagaaaat tttgctcata agtacggcgt aaaattaact ggtgattaaa aaatattagg
     4261 aggacttaac gatgatgcaa tcttcgttac caagcaagac aataaagaac agcaagaaaa
     4321 aagcaacaga tacgtctcat aaaaaaagtg cttatggatt ggtcacacct tttgttattt
     4381 tctttattgt gacgattttg attcccatca ttatctcgtt tgcttttagt tttatgaaaa
     4441 ttggctactc atttgaattt gttggatttg acaattatct agctatcttc cgagatccat
     4501 tgtttattaa atcttacacg aatgttttga ttatcatggc aggatcaatt ccaataacga
     4561 tgctattagc tatttacttt gctgttttat tgaattcagc acagttaaaa ggaaaaggat
     4621 tttttagaac tgtctattat attccaacca ttacatcaac ggtggctgta gcttccgtat
     4681 tcatgacttt tttcaatccg actgggttat tcaatggatt tttgaatgcg ctaggaatta
     4741 acggtgtaca gtggttaaca aatccttttt ggatacgaat atcaatgatc atcacgatga
     4801 tttggcttag tgtaggatat aacactattt tatttttagc tggtcttcaa gggatttcta
     4861 aagagatata cgaaagtgct gaaatagatg gagcatcgaa atttcggcaa ttctttttat
     4921 taacgatccc catgctaaag ccaatcattt taatggccac agttttagcc actatcaatg
     4981 gcttgggtaa tttcaatatc ccaaatatat tctttgggac ttccaatgga ccagaaaatt
     5041 cagctttggt cgtaggagtc aatttatata aaacaagttt tgaaatggtt gattttggaa
     5101 aagcctctgc tattgcctgg acgatggtgt ttgtatctgt tatattatcg gctattcaat
     5161 ttaaattcgg aggtgataaa aatgactaaa caaaagatac aaagcagaat ttttacgtac
     5221 gtcattattg tgtttggtgc cttaatattt ttgatgccat atttatatat gattgtagct
     5281 tcaacgcaaa gtaatcaaga aattttaagt ggcggaatca atttcaagtt tggggatatg
     5341 ctattaaaaa actggcaagc catcatggcg agatataatt atcccttgat tatttggaat
     5401 tcgattgtta ttgctgtaat cagtacagtt ttagctacag ccagttctac aatggcgggt
     5461 tatgcattag caaagtatcg ttttatgggg aataaactgt tgttcaatac agttatgcta
     5521 tctaggatgg tgcctttttt taccacattg attcctttat tttacattat gagtcgttta
     5581 ggattatcga atacttatgc aggtgtgatc atcccttcga ttgccagtac aacttcagtg
     5641 tttatgatgc gacaatatag cagtcaattt cctacagcac tgatggaggc tgccagaatc
     5701 gatggtgcca atgagtgggt gatttttacg aaaatcgctg taccgatttt atcacctacg
     5761 attattacta gtggtttact tatctttatg ggaagttgga atcaatatct atttccatta
     5821 gtcatgcttt cagataaaga taaatatacg gttcccttaa ttatcaaaaa tatgacggtt
     5881 tctagtacag gtgagccaat taattatggc gcaattatgc taattttagc aacttcagtg
     5941 atcccaatgg tcttgattta tgtgtgggcg cagtctaaat ttaaggagaa tgacattgga
     6001 gctgcaaata agtaaaaaaa ataacgaatg ttaaccatct agttcgttct aggagcaact
     6061 ccacattcat aaaaattaaa agaatggcgg gatatccatg aatgaacaga cgaaggaatg
     6121 gtcaagaaaa attttgaatg tagcttctga aaagatagat gcggaattag acagaatggg
     6181 gaataaaatt ccatatattt cttacaacgg aaaatataca gacgtaataa aggacagagg
     6241 aatcgactgg tggaccaatg gtttttggag tggtatttta tggcagctct ttcacaagac
     6301 aaaacgagaa aagttcaaag aggcagcaat acaacaagaa caattattag aatctgccct
     6361 gtaccatttt tcagacgttc atcatgatgt aggtttttta ttcttaccat cggctattgc
     6421 acattatcaa cagtctcaaa acaaacatag tcgacaacta gggttacatg cagctacggt
     6481 acttgctgga cgctataata tttcagggaa ctatttagta gcatggaatg accataaacc
     6541 tggttggatg attattgata gtatgatgaa tttaccatta ctgtattggg cttcgaggga
     6601 aactggtgat ccgaggttct ctaaaattgc tcggcagcat gcagatacaa cagctaagta
     6661 tctagtgagg aaagatggat caaccgggca tatcgcagaa tttgatgcag aatcagggaa
     6721 atatattcgg ttacttggcg ggcaaggata caatgagaat tcttgttgga gcagaggaaa
     6781 tgcatgggct ttatatggat tcgcgttaag ttatcgttac acaaaaaatc cgtactatct
     6841 agctatagca gaaaagatag cagtaaattt tattcagaaa atcgaaaaaa ctggttatat
     6901 tcctctcatt gattttgctg taaaagattc gaatattgat accagtgctg gagtatgcgc
     6961 tgcatgcggt cttctggaat tagcaggata tgtttcctct gtacgatcaa aaatgtatca
     7021 tgaagctgct atcaatatac tccaaaagac agaaaagcac tatgcaaatt ggaatatgga
     7081 tgaagatggg atcattggag gcggagcaga agcataccat agagaactga cttataatgt
     7141 gccactgatc tattctgatt atttttttat tgaaggctta cttcgattag aacagtcgga
     7201 attagctatt tggtagtaaa aagaggagaa aatacaatga aacatccagt accactatta
     7261 aggaccagac aggattactt tgacttattt aaaaagataa atcaaccact ccagccgttt
     7321 tatcgagcaa gtggcgcaca aattgattat atacatcaag gggtaggata tggaaataaa
     7381 attgcaggaa tggaaggatt ctctcgggtt ttatggggag cggggcccgc aattgaccaa
     7441 ttagatgaac gatggttgaa attaattcta tcaggaataa aagctggaac agatccttcc
     7501 catcccgatt attggggaga aatacatgaa agagaccagc gtatggtaga aatgcccgca
     7561 atgttattag cgttatatca tcatgaacaa ttattatgga aaaaattaga tacaggagaa
     7621 aaaagccaga ttattcattg gttctcgcaa atttttgaat atgaatgtgc agatggtaat
     7681 tggcaatttt ttaagataat tgtcggaaca attatccaaa aatttggtta tccagttaaa
     7741 gaatcagcac tgaaagaagc atttgataat atagaaaatt gttatcttca aaatggctgg
     7801 tatcgagaca gttcaagagg tcgccaagat tattataatc cttttgcatt tcattattat
     7861 ggtttgattt atagcgtact agaaccagaa aagcccatta gccagatata taagaaccga
     7921 gctattgatt tttctagaga ctatattcat ttttttgcgg aagatggtgc aaatgtgcct
     7981 tttggtcgaa gcatgattta tcgttttgca gtttcaagct tttggtcagc tatgttgtgg
     8041 gccgatcttt tgccttttaa attaggtgaa atcaaggggt tagtcaatcg gaatataaga
     8101 tggtggatgc aaaaagaaat atttgatgaa aaaggtatcc tgaatattgg ttatacctac
     8161 cctcaattga cattgactga accctataat agtacgttat ctccattatg gctgaataag
     8221 atttttttat tgttggcgct acctgaagac catgagtatt ggacggctaa agaaatgcct
     8281 atgcctattc gtgaaggaac taaacttctt tcagaagcca atttgctcat acaacatgat
     8341 aatggacata catttttttt gaatgcggga caattagggc caaatttcca tacattgacc
     8401 aatgaaaaat atttaaaatt tgcttattct agtcaatttg gtttttcgat accgcgagcg
     8461 aatcaattga aagcagaaat ggcaatggat tcaatgctag ggattcagcg agcggacaca
     8521 tatatcacga ccagttttaa gaatgagagc atcaaaacct atggacagtt catcgttcga
     8581 aataatgtac gagacattgt atgtaagaaa gaggtacttg cctctacatg ggaacctact
     8641 ttacaggcaa aaatcagaac ttggcttatt ccttttgaag gctggcagat tcgcgtacac
     8701 aaagtagaat tagatacaga atatgttcta tatgagacag ggtttgctat tgaatgttct
     8761 cctgagaaag aaggaataat catagaagaa gacaaggaaa actatagggt gagtgaagct
     8821 ggcttttcag gtattatctg tttaagtgac gatactatta aacgaaaatc cacagctatt
     8881 atgggtttgc ccaatacaaa cttgatgaca tgggaaaaca cttttattcc tggattagag
     8941 ggtacatttg gaaaaggaac ccattggctc ggaacaggag tggttgctca tcaagatcgg
     9001 gattatagtt tagaagtatg gaacaatcgt cccaaaattg attttgatgg gacaaccggg
     9061 ctaactatcc aaaataaaaa caataaaaaa agactaaaat tatgctaaag gctggtgggg
     9121 aaatgcttga taaagtgatg tttgaagaga taatacatcc agaaaaatgg ataaatgaat
     9181 gttataaaga attaatggaa aaagaatgga ataatgagat cgagcgttgt cgcagacaca
     9241 cggagaggtt aatcaaacgt atcagtaaca gtcagtatca attcccaagc gagtgtccta
     9301 taaaaggacg ttatccatta agagacaata ttggttggac agaaggattt tggacaggaa
     9361 ttaattggtt gttatttgag aagacgggtg atcctttatt tcaacaaaca gctgaaaaag
     9421 cagatataga ttttatccac aggattgact gtcaaattat cactaatcat cacgatcttg
     9481 gttttttgta ttctttatca accgtagcag attataaaat aacgggaaat cgaaaaatga
     9541 aggaagcttc aattgatgct gcagaaacat tacttcagcg tttttgggag aaggcaggag
     9601 ttatacaagc ttggggagat cctagtgatc cttcacaaca aggtagaatg atcatggatt
     9661 gttgcatgaa tcttccatta ttgtattggg cgagtgaaga aactggaaat caaaaatata
     9721 aaaaagctgc tcaggctcat attcaaaatg cagcagatta tttagtaaga gaagatggtt
     9781 caacttatca tacattttat atggatgtca aagatggaaa accaagatat ggaagtacgc
     9841 atcaaggtta tagtgataat tcctgttggg ctagaggcca agcatggggg atttacggtt
     9901 ttgcattaag ttatcgttat ttgaaagaac aaaaatattt tgatttaagc aaaatattag
     9961 ccaattattt tatgaatcgg ttgccagaag ataccatttg ttattgggat ttgatctttg
    10021 atgattctag cagagaagaa aaagatacat ctagtatggt gatcatggtt tgtggattat
    10081 tagaattaat agaatcaggt ctattagaca aaacagaaaa gaacttatac cagtctgctg
    10141 tttggaaaat tatgcgacaa ttgaaagaaa gatatgctac aacggaagaa gaagatggct
    10201 ttttaacaca gtctgtttat aataagccca aaaataatgg ggtaaataca ccttgtattt
    10261 ggggtgatta ctattattta gaagctttag tgagaatgac tagtaattgg tcttcttatt
    10321 ggtgaaaaag gtaaatatgg aatttttgtt tgataaaata aaaaaagaaa aaccaaaaca
    10381 gcatgaatgg tttctgtttt ggttttttcg ttaattttca tattcttcta attccaagct
    10441 tagttcttcc cattccgtca attttgtttc ttgatcttgt ctagcagctt ctaaatcttg
    10501 gttcagctca ttgagtttta cgtgatcttc caggttttct ggcgcgacca tttcttgttc
    10561 caactgatcg atccattcat cgatccgcgc catttcttct tcaacctgag taatttttcg
    10621 ttgaaggcta cggataagct tttgctgttc tttgctttgg taataatcag atttgttcgt
    10681 gggagcagga gcagattctg gctcatcttg tgccaacaat tcggcgattt cttcttcttc
    10741 ctgctttttc tctaaataat aatcatagtc tcctagatag actttgctgc cattttcaga
    10801 gagttcgacg acttttgtcg cgatccgatt gatgaaatat cggtcatggg aaacaaaaag
    10861 cagtgtgccg tcataatcga tcaatgcatt ttctaatact tctttgttgt caatatctaa
    10921 atggtttgtt ggttcatcca aaatcaaaaa attttctttg ttcatcgcta acttggctaa
    10981 agcaacccgt gctttttcac cgccacttag cagagggatt ggtttttcta catcttcgcc
    11041 agaaaataag aaagcaccta aagcacttcg gatctctttt tctggagtgg taggatgctc
    11101 atcccacaat tctgctagga tcgtcttgtt tgtatgcaga tcagcttgac cttgatcgta
    11161 atagccgatg gtgacatttg tccctagcgt ttcttttcca cggataaaag ggatctggtg
    11221 aatgattgat ttcagcaacg tcgatttccc gataccattc ggacctacta gagcaatcgc
    11281 ttcttgacga cggatatcta gattgattgg ttcagataag gtgacattat catatccgat
    11341 agcggcatct tcgatttgca agactacatt tccagaaggc ttttctatac tgaatagaaa
    11401 gtttgcttgt ttttcatccc cttgaggacg atccagccgg tccatttttt ctaatgtctt
    11461 tctgcggctt tgcgctcgtt ttgtggtaga ggcgcgaaca aggttccgag caacgaagtc
    11521 ttctaattta tggatttctg tttgctgctt ttcataagcc ttccattcac ttgctaactg
    11581 ttctgctttc aaatctagat atttcgaata gttacctttg taatggcgca tttttcttct
    11641 gcttaattca tagacttcat tgactacttt atctaaaaaa taacggtcat gggaaacaat
    11701 caataatgca ccagtatagc tttgcagata attctcaagc caggaaagtg tctcgatatc
    11761 taaatggttc gttggttcat ctagaatcaa aatgtcaggg tgttgcaaaa gcatgcgagc
    11821 taaagctaaa cgtgtgcgct gtccgcctga taaagtatcg attttctgat cataaaatga
    11881 agaatcgaat ttaaacccat gcaatacgga acgaatttca ttttcataac catagccgtt
    11941 tcgatctgaa aaatcgtgtt gcagccggtc gtattctttt aaataagaac tataagcagg
    12001 tgtgtctggc gtaccttcgc taatgaaaat ctctagctcg cgcatccgct cttccatttg
    12061 tctaacttgt tcaaaagctt tcagcatctc gttccaaact gtttcattgg aatcaagtcc
    12121 tgtgtcctga gcgaggtatc cgagagtcgc tgttttattt ttagcgatct ttccttcatc
    12181 aggtgcttca attccagcaa tgatctttaa taaagtcgat tttccagcgc catttctgcc
    12241 aactaaagcg acacgagcgc ctgtactgat ttctaaatgt atgttttcaa ataagacttc
    12301 tgcaccaaaa tggcgagcaa tctgattggc ttgtaataat atcatggaat cctcttttct
    12361 actttgtgaa ttctcctcaa ataaaaaact atttttcaat tagtagtcta ccataaattc
    12421 cattatactg cgagatttgc agaaatgata aataaatgaa taaagaataa aatttcacaa
    12481 tatctgagca atggaattga ttttttccta taaatactgc tatgatggag aaatcaaaga
    12541 tgtaattaga caatacgtgg aggtcatttc gtgaaagatc atattattcc aaaggcgaca
    12601 gctaaaagac tgcctttgta ttatcgctat ttaaggattt taaataatgc tggaaagaaa
    12661 aaagtgtctt caactgaatt aagtgaagcc gttcaagtag atagtgcaac gatccgtcga
    12721 gatttttctt attttggcga attaggaaaa cgtgggtatg gttatgatgt agaagatctt
    12781 atgcactttt ttgcaaagac attaaacgac gatcaattga caaatgttgc gctgatcggt
    12841 gtagggaact tgggaagtgc tttactaaaa tacaaattcc atcaaagtaa tagcatacgt
    12901 atcagttgtg cttttgatgt gaatgaggaa atcgttgggc gcatcgtaga cggtatcccc
    12961 gtttatccaa tggaagatat gtcagaacaa atcaagatcc aacaaatcga agtagcaatt
    13021 ttaacgatac ccgctgaaag agcacaagaa gtagtcaatc agttaacgga agcaggaatc
    13081 aaaggaatct tgaactttac cgctgctcga ttgaatgctc cgtcagatgt attgatccaa
    13141 aatgttgatt tgacgaatga attgcaaacc ttgatctatt tcttgcattc tgacgcaaat
    13201 gcgcattccg aaaatacagc agaataaaaa taaaaagcga ttgaacaaat gaaggcaaaa
    13261 ctttcacttg ttcaatcgct tttttctgtt tggacaacga ttattttgtt cggaagattt
    13321 cttcataaga aagggaatca tcttctgctg tcagttcatc tagtagataa cggatttttt
    13381 taaagtcgat gaaccgtgct tgccgaaaga cttcttttcc ttgatgaaaa atcaggacaa
    13441 ctggcgcagt cagcacttca aatttactag ctacttctgg tgtttcaaat gcatccaaat
    13501 gaagtgccgg aatatcataa tgtgtgagaa gttcctctag tcgtggacga acagcgtgac
    13561 aaacactgca attaggcatc gaaagataaa tgacggcaac ggatgactgt tgcagtatgt
    13621 cttgaacttc agtcaagtta gtggtttgtt tcatttttat tctccctttt atgatagaag
    13681 tgatatgtat gagaccttaa tttaatggga ataacagaaa aagtcaattg aagtgctttg
    13741 gtaaaaaata agagttgtgt aaaaatttac agagcaactt gttttttttg aataaaagcg
    13801 tatatttgca aagcgaatga actagaaata aaaaggagga aactcatgtt ttcttttatt
    13861 ccaaattcgg ttttgcagat gagtacgatt ttcttatcga tcgttattga agcattacct
    13921 ttcgtattat taggctgtct tatctcaggc gccttacaag tatttttgac cccagaacgt
    13981 gtgaagagat ggcttcctga aaatcgtatc ttctctattt ttactggttc gatcttagga
    14041 tttttctttc cttcatgtga atgcggcatc gtaccgatcg ttcatcaatt tgttaaaaaa
    14101 ggcgtaccag ttcacacagc gtttgctttt atgctgactg ctccgatcat caatccaatc
    14161 gtgatttttt ccacttttat cgcatttggc aattcttggc agatggcaag ctggcgggtg
    14221 ataggcagct tttttgttgc ccttgtgatt ggtgtctggc ttgcctattt tcaaaaagac
    14281 gctgtattga aagaaaaaat cacgtctaaa gtagcagaaa atcaccatca tcatgtccat
    14341 gaagaagaac aaagtgagaa gaatgaacca aaaaatcagc tgatccatca aactgggcat
    14401 gtgttgacac attcaatcga cgaatttttt gatacaggtc gttatttgat cattggcgga
    14461 ttaatcgcag ctgcgatgca gacttattta ccaactagaa ttatgctaac gctcggctca
    14521 acaaagattt tagccattat catcatgctg ttattggcct ttacgatgtc tctttgctca
    14581 gaagcagatg cgtttatagg atcttctttg ttaagtctgt tcggcacagc acctgtagct
    14641 gcctttttgg tctttggccc aatggtcgat atcaaaaatc tactgatgat gaaacgatat
    14701 tttacaggac gctttatttt atcgctgatc ggactgattg cccttagcgt tattggattc
    14761 acattattta tttaggaggg aaaatatgct gcggtttttg attttatctg ggtatttcga
    14821 attgatgatg tacttgcaag tttctggcaa actcgatcaa tatatcaatg tccattatcg
    14881 ctacttagct attctttcaa tgattttatc tgctcttttg gctttagttc agctgtattt
    14941 gtgggtcaaa aacggaaaag cagaagaaaa ggcacacgag cacgatcatg accatgggtt
    15001 gtcaaaacct tatcaacgtg gaatcgctta tgtactgcta gctttgccag taatcgtggg
    15061 gacgttgttt ccaacagtta gtctcgacac tacgatcgta gaagccaaag gatttaattt
    15121 cccagtaagt aaagaatcag taggggatcc ggagatgcag acacaatact taaagccgga
    15181 taccagtatt tatttcaaca aatcagacta cttagaccaa atgaatgagt tgaaagaaaa
    15241 gtatggggat aagcaaacta tcaagatcac agatgaaaac tatttagaag ttatggagct
    15301 aatttataat taccctagcg aattcattgg caaaaaaatc agctatgaag gatttgttta
    15361 ccaaacaccg aacgatagca atacagatat cttcttgttc cgattcggca ttatccattg
    15421 tgtggcagat tcaggagtct ttggtttgtt gacgcatatg ccggaaggag taacggttaa
    15481 aaatgatgaa tggtataaaa tagaaggaac catccaatca gactattatc aaccattcaa
    15541 gcgtgaaatc ccatctgtag ttgttacaaa cgctgagaaa gtctctgctc cgaaaaatca
    15601 gtatgtgtat cgttcttttt aaaagaacaa aataaataaa catagctcag acagctgaca
    15661 cgcaaggacg tcggttgtct ggcttttttt gcatgatgta ttcatttgtt tgatatttat
    15721 tttagaagca gcatcctgaa agtatttgat atcggtttca aaggtgttgg aaaagattgt
    15781 gaagagaatt acaatttatt cctaacatgt atgttgaaaa gttgtatgaa atgattttga
    15841 tagtaaaata tcgagtggag gtacttttta tggtaacgcg tatatttatt ttggatgata
    15901 gtttggtttt tgaaaaaatg ctagtagaaa ttttggaaga atccagaatt ttattaagtc
    15961 cttggaaatt tgagatcgtt cagggattga acgttataca atttgttgaa ttcattaaaa
    16021 ataatgaaat ctattcctca gatatttttt tcctagacat cgatttgaac gcctattata
    16081 gcggtatcga actaggcgaa atgcttagag aaaagaatga aaagtgtaaa attgtttatc
    16141 taacgtctca tccgaataaa gcaataactg ttattaatcg aaagattacg cctagtggtt
    16201 atttgataaa agaattcact tatgaaaaaa ataaaaaaag catccaaagt tacttattga
    16261 aagaaaaaga aaactatcct gttcaacatg ctacgaaaaa aaggtggaca actgtccgtt
    16321 atggtaatag cgatcactat atccattacg atgaaatcct ttatctaatg agcatgccag
    16381 gttataaaaa taaaataggc ctagtattga aagatgaaga gattctcgta aacggcacaa
    16441 ttaaaaaata taaaaaatca ctcgaatatg agtatttatg taaagattta aaagcttata
    16501 tcattaatac tgaaaacatt acatctatta gcagagtaaa cgaaatagta acatttgcaa
    16561 atggagaaca actagagatg ggaaagagaa gtattgataa actaaagaat tttatgcaag
    16621 aaaagataga aaatgaatca ttttaaagaa aacaaagact catatcttac ctgttagaag
    16681 agtgttttcc tgtagaagag aacaacccaa gtagcagcct agtcgtttct atcctttttt
    16741 gttatgcact ctttttgaca tttactccac caataacgaa gggtacaata aaaagtaata
    16801 acaaacaaaa ggagtacaga aatgattgaa ttcatcaatg taagtaaaag ttacgggaaa
    16861 aaacaagcat taaaagactt gtctctctcg ataagacaag gggaaatttt tggattttta
    16921 ggacataatg gtgccggaaa atcaactacg attaaaagtt tagttagtat tattgaacca
    16981 tcatcgggaa cgattttagt agatggaatg aaattgacgg aaaatcgatt gagtatcaaa
    17041 caaaaaatcg gttatgtgcc tgattcccca gatatttttt tacaattgac ggctggagaa
    17101 tattgggact tgatttcagc tgcctatgaa ttagacacac aaaaaaaaga aaagcgttta
    17161 gcagaattga cagcgctttt tgatatgtat agtcatcaaa atgaaacaat cgcaagtttt
    17221 tcacatggaa tgcgtcaaaa aacgattttg atcggcacgt tgctgccaga tccagatatt
    17281 tgggtattgg atgaaccttt gcaaggactt gatcctcaag cagcatttga tttgaaagaa
    17341 atgatgaagg ctcatgccgc aaaaggaaaa acagtcattt tttctacaca tgtattggat
    17401 accgcacaac agttatgcga taaactggct attttgaaga aaggtgaatt gatttatcag
    17461 ggggcagtta gtgaattatt gaatggctca cctgatgaaa cgctagaaaa gatttatttg
    17521 aaaatggctg gaaggcaagc gacaggagat ggaccgtatg tataagcgac aattattaga
    17581 attaattcga gttaatcttt tatatgttaa tccacagctc acgaataaag caagagagaa
    17641 aggaaaaaag ggaaagcgat taactatcta tcttttgtct caaaatcttt tttcagggct
    17701 gattttttta ctgatttacg gatttgctat gtttgctatg aactttgtta aattgccagg
    17761 tttctttact tattacgtgg cactttttgg tatgttaggt ttaactcaaa gtatatcagt
    17821 catttataat atcttttttg aaggaaatga cctgcaagta tttttgcctc tgccttttgg
    17881 acaagaacag gttttttcag caaagatttt ggtagttgct ttgaccatca ttccttttgt
    17941 ttttccgctt ctagtcatgt ttattttaac cggatggcga gctgatctag ggattttgct
    18001 tactatcatt atctcaatcc ttttgttttc tctatttttg attattcttt tttgtctggc
    18061 tagtttgatt gtttttggtt tggctcgaac agcagtgttt aaaaaacata gaaaaacagt
    18121 ttctagtatc ttgcttggca gctcgatgct gatcgcattt gctggaatca tgtggatgaa
    18181 tcatcaaacg gggactattg aaaatgagtt aacagatcgg catccgattt cgatattatt
    18241 gccattctat gatgctgcca gtcagccact ttctcaacaa ggtttattgg gttggggaat
    18301 actagctatc acggctctgg gatttttcct tgctgtaaaa cagctgatag taccaaagct
    18361 tacagagcaa ctgactgatt caaacagtga gttcaaaata aaaagaaccc ataaaaagaa
    18421 ccaaaatgtg catcagcttt tatttagtta caatgctcaa ttagtaaaag aacctaattt
    18481 gattatgcaa gttctttctt cttccttgtt aactccaatc atttttattc ttgcttttgc
    18541 cctaggaggc gaaataaaat taactgattt agataataag ttaatcggtg ttgttttcct
    18601 ggtagggatc gcattggctt tcttaacagt taatcagact tcttttatca gcaatcttat
    18661 ttctttggat caagaaaatt ttctgtttat ccgttctttg ccaatctcta tgaaccaata
    18721 tttaaaagaa aagttcagat tcggatggat attgcagagt tttttaactg gtgggatcgc
    18781 attattaagc ggactgtctt ttcagctgcc tttgtttttt atccctagct tgctatttgg
    18841 ttctttattc ggctgctact tattgtcact acgttatttc gctagagatt atcggttgct
    18901 gctgctgaac tggaccaata ttaaccaatt atttaacaga ggttcaggaa gtttgggttt
    18961 agtcgcaact atgatgggtt cattgatcat cagtattatt cttatcgtct tatacggaat
    19021 gttggctttg ttttatccat tttggttagt gaatattcct gtgatcggtt tagttctaat
    19081 actttccgct ctatggatcg tttattatca acgaacgttt tggaaaaaat ttgaataaag
    19141 aagaggggcc gtgacaaaaa tcatgtcgca gccccttttt tacagataga gggtgttcaa
    19201 aagtcagttt ctcagtatta agtcaatttt atgtaaaaat gtgagaagct atttttacaa
    19261 aacttttttt aatactcata attacttctt attgctcctg cggttacttg tcgcaatgtt
    19321 tgtgagatat tttgtcatcg ctcaaggctg aacgtctctt tcacaacctt ttctttgtta
    19381 cacttcgttt atttaaaaag ccgttttttt cgtttcatca tgattgcgtc acgctttact
    19441 ttgaaattac ggatgttatg gttaaagact tctgataata caagacctaa agcgattgct
    19501 ccagcaatca taattgcttg gaccgcataa tgagccgctt ctcgatagga ttcagtaacg
    19561 agatttcgaa ccgcttgata agctaaacca ccagggacta gaggaaccat cccgggaata
    19621 ttgaaaatcg ttacgggcat tttcaaacgt ttagagaaaa gatcgctgat gaatgctact
    19681 cctaaagaac caagcaatga accaaaagca gcagatccgc ctatttgaac acagatccag
    19741 tagatcatcc agccaaaagc accagaaaga ccacaacaaa tcaatgaacg cctaggtaca
    19801 tttgtaataa cggcaaatgc cgcagtcgct aaaaaactaa aagaaaactg aataaatatc
    19861 gtccccatac ttgtgttcct ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa
    19921 ccgatcatcg tagcagtcat catcgcttct gttccgcgag aaactcctga gacataatgt
    19981 ccagctagca aatcacggat agcgttcgta atttgaactc caggaactaa aggcatcaca
    20041 catccgataa tgattagatc ctgattgctt cctaagccaa aacgtacgct gtagatagcg
    20101 gctatgccaa tcaataaaga agataaaaat tccgataaaa atttgattct aaaaaatttc
    20161 aaactatata gataagctgc gtatcccaat cctccaatga ctaaagtcac aggtaaatcg
    20221 gtcatctttc cgccaaataa caccataatt gttccactga tcactgccgc gctaacgatt
    20281 tgcaaccaga caggaaagaa agaacggtct cggtcgattg cttgcagttc gcagtaaagt
    20341 tcttctaatg ttagctctga agcaacatat ttacgtgaaa gatcatttac ttttactact
    20401 ttttctaaat taatcgaacg gttggtaatt tgttccattc ggatggtcga ggtacgatca
    20461 agaccaataa agagaccggt ttgagtgaca tagctgacca aacggaaatt tccagaagct
    20521 aaagcaattc gactcatcgt atcttctacc cgatacatct ccgcattgct ttccatcatg
    20581 attttcccag cgagtaaaca agtgtccagc aacagatcta attcttttcc cgtcatagca
    20641 tcttctccaa taattgattg attagtttta ttttactaca aaacatacca gaagcgtaaa
    20701 gaataaaaaa acttgaggaa ggaataaaag aggcgtatac tgtttttgta ttgcgggtgt
    20761 agtttagtgg taaaatccca gcttcccaag ctgatgtcgc gggttcgatt cccgtcaccc
    20821 gcttaaaata ggttgtgaaa aagctgctct gcaacaagta ataagaacag ccaagcaaaa
    20881 acaacttctt atgtttttaa cttggatgga cttattaccg cagatgcaag cttttgcgca
    20941 ccgtttacca agcggttgtg aaatcagtgt attgtcttga gtatgaagaa aatttttgaa
    21001 taataataaa taccttacag atagagtggt gactgattag agacgaggaa tccgtctctt
    21061 ttttgtattt ttaagccatc ttaaaacagg ttttattttt tgctgtttct atttctttct
    21121 agtatttatt tataggggaa catatgttaa gatttaggga gctgttagta aaggagtaaa
    21181 gaaatgagca aaaaaacaac caaggcgatc gtggggatcg tcgctgctgt gattgcaggg
    21241 gcattcggtg tttccaccac acaaaaagat tcagatatct ttcagcagat tacaactatt
    21301 tttgattcca caccacaagc ttctcaagta gcggatgtgc cagaatatga tggaaaacat
    21361 caagaaatag aaatcaatgg gaatcagccg gaatttacaa aagaggatct tagcctttcc
    21421 aaaggaacat gggaaagcta ttcaaatatt gataaattga acagagtagg acaagccaat
    21481 gctatgcttg gaaaagattt gttccctact gaaaagagag agcctttgta tattgacccg
    21541 acaggttgga aacaaaagaa attaagtgac gggcaatggt tatataatcg cagccattta
    21601 attggtttcc agctgactgg tcaaaataac aacattaaaa acttgatgac agggacacgt
    21661 tcgttgaatg ctccatacat gcttgctcac gaaaatgata ttgcggcata tatcaaagaa
    21721 acaaatcatc atgtacgcta ccgtgtcact cctcattttg aggggaatga actggtagcc
    21781 aggggagtgc aattggaagc agaaagcatt gaagacaaaa aaattgaatt caatgtcttt
    21841 atctacaatg ttcaagatgg ctacacgatc aattatgaga caggacaagc aacaaaaaca
    21901 aaaaattagt agcagctatt cgaatttttt atgaatatta tgaaaaaatt gtgtaaaaac
    21961 atacattcaa agaaatatag cgtatacttt atgaaaagag tacttttgat aaagctacag
    22021 aaaaaaggaa aaaaacatac aaatagtgtt tcttaattta ttcggaagtt tggaatattt
    22081 gtttgtagtt ttccctttct tcgagtaaaa taaggttgta gaaggtaagc tggaattacc
    22141 ttgcgggaaa gaggtgtaga atcatgggtt ttacagatga aaccgtacgt tttggttttg
    22201 acgatagtcg caaaaaagaa gtgagcgaaa cattagcgat tgtttatcgg gctttggaag
    22261 aaaaaggcta caatccaatc aatcagattg tcggctactt gctttctgga gacccgtcct
    22321 acattcctcg ttatcgtgat gctcgaaatt tgattcgccg tcacgaacgc gatgagatca
    22381 tggaagtaat tgtcaaggac tacctctcaa atcatggagt gaatttatga gagtaatggg
    22441 cttggatgtc ggttctaaga cagtaggtgt tgctatcagt gacccctttg gctggacagc
    22501 tcaaggagta gagatcattc ggatcaatga agcagaagaa gagtttggct ttgatcggtt
    22561 gaaggaatta gtagcacaat atgaagtaga ccggtttgta gttggattac ctaaaaatat
    22621 gaacaattcc atcggaccaa gggcagaagc ttccatgtca tacggagaaa agatcaaaga
    22681 actattctcc ttgccagttg attatcaaga tgaacgatta accactgttc aggcagaacg
    22741 tatgctagta gagcaggcaa atacttcaag agccaaacga aaaaaagtca tcgataaatt
    22801 agcagctgtg atgatcttac aaaattattt agacggctca ggtaagcaat tattttaata
    22861 ataaaagaaa agaggtaatc ttatgacaga acataaccat tcccacgacc atgaaggaca
    22921 cgaacacatc acattggtgg atgaccaagg aaatgaaaca ttatatgaaa tcctactaac
    22981 agttgacgga caagaagaat ttggacgtaa ctatgttctt ttatatcctg ctggtgtttc
    23041 agaagatgaa gatgtagaat tacaagctta tgcatacatc gaaaacgaag atggaacaga
    23101 aggcgagtta gaacaaatcg aaacagacgc agaatgggac atgattgaag aagtattcaa
    23161 tacattcatg gcggaagaag aagaatagac tataaagcta ggagaaacct tgatatgata
    23221 gggtttctcc tttttttatt ttttactgta tgccatattg tatgccatct agtcaatatt
    23281 taagtgttgt actaattttt caacagtctt ttctttcgtt tcttttgcca agtgaccgta
    23341 acaatcgatc gtctgactaa tttttgtatg ccgtaatcta gtttgaactt ctaccatagt
    23401 agctccagcg tccaagagca ttgaagcatg agtgtgtctt gctacatgca caccgagaaa
    23461 tggaaccata gctttttcac aaatagtaat caatcttgca taaatagcgt ggtgtgtgat
    23521 tagttcttta tagataccac aaaaaatcat agtcggatca gttacaccaa gtgtcagcat
    23581 atatttccgc tgattaatct gccatttttt taatatagaa attgttcttt catctagtgg
    23641 aagaacccca gcactacaat cagttttagg atcactgatt ttatagccat ttttggattg
    23701 gcttagtgtt ttattgatgg tgaccgtatt ttcttgaaaa tcaatgtctg accaattcaa
    23761 tgcaagggct tcactgatcc taaccccgct ataaaagaga aagcgtagta acgttttatc
    23821 aaagtcgttt cgataagttt caggtttgct gtttaggtaa tcaaacaagg tgatcagttg
    23881 ttctttactg taaactttga ccttttcttt atgcttagtg gtggttcttt taggcattaa
    23941 tacgttgtct agtggattag tctcaataat cccaagattg atagcgtagc taaaaatacg
    24001 cttagaagtt gaatgaagta ccttgtattg gtcagtagtt tcagcccagc tatttacaat
    24061 ttgttgggct ttttttaccg tgacttcatt cacacgaaga tttccatatt ttggaagaat
    24121 ccactttcta aacttgattt cagttttcat gaaggtggct tctcggacag tggttttata
    24181 tgtttctagc cacaagtcat aaacttcttt aaacgtttcg ttgttcgctt ttttaatacc
    24241 gtttttatcg tagtccactt gcaagcgtga caaggcaagc tgggcttctt tttttgtttt
    24301 gtaacctcgt ctacgaacct tatcaggttt gccagtcatt ttgttcattc caagataggc
    24361 ttcatagagc caaaatttac cttttttagt ttcatattgc ttgaatgttg ccataatagt
    24421 tttccctcca tagtgtagca agccgtgggg gcggttggag gaggcagttt attattattt
    24481 tgaatagtgt ttatttagct cagtaacaag agctgtgaac tcttttagaa gatcattttc
    24541 ataaaattct tgatactctt ttttctcatc tgtatttttt tctgaatcat taattaatga
    24601 gtcatatcgt aaaagcgtat ttagaagagc tgcgagataa gcaattgggg tactcccgtc
    24661 aggaaattca attcgagatc ctgttttaag atattttatt gcgtttgtta aataaatctt
    24721 ctgtgagaca gtcaggttat cttcttcgat gttattgata gcattagaaa gattagaatc
    24781 aattatagag ttattttttt caatagtttt tttcaattgc ttttcaagca catttcgctt
    24841 aatttcgggc atcatatgca tatctcttaa tgtataagat ccgtttaata gataggtagt
    24901 ggatacatct aatacctttg aaagttgttt aagcctggtg ttattgggaa gattatagcc
    24961 attttcccaa ttactaacgg cacctttact ggccggtggg gataatttac ctccaagttg
    25021 ttccattgag agtccctttt caattctagc cagtctaatt ctttgtcctg tctccactgc
    25081 ttcggggtat ttttttctag ccacattttc accaccattc atatattttc tctactatat
    25141 cacgatttaa atatgtgtga aacataaaag ttcaaaaaag tatgttaaaa ttcttgcaaa
    25201 tgaaaaggta tgatggtata ttgagtttat aagaggtata gaaatgtatt caagagcctc
    25261 ttgcattttt ttaaaatctt tgtatagaaa tgtatgctaa cgagggggga taaatatgga
    25321 attaaaaatt gacaaactca cgcttgatga tccagctttt aaaattattg acgatcgagt
    25381 tgttcaacaa gtagagcaaa ctcaaggaac gttactttca ttattagaaa aaaagtacca
    25441 gtttccaaaa tatatgaaca aggcacaagc tgctaagtat atgaacgttt catacaatac
    25501 tatgatgcag aaatatgttc ctaacggttt aaagttgatt atagttgatg gagtcgtaag
    25561 aatttcacaa gatgaatgtg atcgctttat ggaagaatac caaaaataaa cgcaagccgt
    25621 gggggcaaag ggattaatag gaggaaataa taatggaatc gacacaaaat aaagcaatcg
    25681 aaaaagtatt atcaacaatt cttacagaag acacagcggc agaattagca aacttggaag
    25741 gcaaagcgct agaagaaacg tttgaatgtt tgtacgaaca aatggactat caaaaattgc
    25801 taccacaaga accaacggta agcggagtat tgcgtggctt gaacgattta gtacaagcag
    25861 agtttaaaga acgactttct attgaagagt atcaagaaat tttgtatcag caagttgagt
    25921 tgttagcgag cttgctaggg cttgaactgg aggacgttga atgaaaactg aaatttggaa
    25981 cggacacatt ataagatttg tcgatattaa tgatgaatgg tgggcagtgg cgaaagatgt
    26041 tgcggaagcg ttaggtctta aacaggttac tagagccatt cactctttgc ctaaagatgg
    26101 ggttactact agtaaggtca tcgattcatt gggcagaaca caagatgtaa acatcatcaa
    26161 cgaaaaaaaa tatttaccgc atggcattca aaagccgtaa aaaagaagcc gaggcatttc
    26221 aagactggat tttcgacatc atcaaagaac tacgccagtc cacaggactt gaaggctttc
    26281 aagtattccg catgctagac aaagaacacc aaaaagaagc aatgactaaa ttaagccacg
    26341 ctattactga accaaaacca gtagactaca ttaaagcgaa tgtgatagcc aataaagctg
    26401 tatcaactat ttatggtcat tccaaaatgg taaaaaagaa agatatgact cctgaaatgt
    26461 tggttgatcg tgaacctatt ttagatgaga cagtagaatt aatgactgtt aaagaaaaat
    26521 atgggttgca gttttcagtg agtgagaaaa tttataatcg ttctgctgaa ttgcagacaa
    26581 cttaggagca ttaacatgat cgagaacaaa gaaaaagcct tatccataac agtcttggcg
    26641 gacaggataa aggcaactta attgggataa aacccgatat tctttgttcc gattatacca
    26701 tattaaaaaa tcggagggaa ccattatgaa acgaaactta attataggaa gcattttaat
    26761 cggcggaatt ttcggcttat taccattaat tgcaaaaata gctatctttg caggattagc
    26821 gttatatctc ttcactgctt atgacgagta cgactatcaa ttaagaactg gagggcataa
    26881 gtagatgtac aaaacaaaac tattaaacca actcgatagc cttgaattag aggaaattaa
    26941 tcaagggatc gctgaactag aaaataatat cgggaaaacc tactttggaa attcattcaa
    27001 tgaaaaacta acagttttgt atgtactcaa aaaacatgca gaacacaaaa taatttgtcg
    27061 agaaatcaac gagctaaaaa atcagatttt aaccgcatgg ttaaacatta ctgatatgca
    27121 agaagcaagg gtgaaaacat ttaatacatg ggtaaagtat cagaatcaat taaaaggtgc
    27181 tgaatttgtt cgggatgggt tgaaatacga acttgagcag ttgaagctca tggaggtgtc
    27241 tgaatgattt ataccagtat tttgaaaaag gaggagaggt ttattggcaa ataattatca
    27301 agcagcaacg gaattacaaa agtcgggatg gtcaatttat cccttatctc ctggaactaa
    27361 aattccaata aaaggctcta gtggttggaa tgatgcaact aacgattcta atcaaattaa
    27421 tgaatggtgg acggaagatg caactagaaa tattgggtta atgcttgaag atcatcaaat
    27481 ggtggtagtt gacctagatc aacattctga aacacgaaac ggcgttgaga attataaaaa
    27541 acttgcttca atgtatgatc catttccttc tacttatacg gaaatcacac caggtaaagg
    27601 aatccatttc ttctttaaaa ttcctgatgg tgtaattatc caacaagaaa caggtgcatt
    27661 tgctgatatc ttcgggcgtg atgataaagg taaaagttta agcgggattg atattatcac
    27721 taaaggagtt ccaatagcgc caactatgat tatttcaaga agtgttggga gagcatatga
    27781 acctattgaa aacgtatctc taaataatat tgcaccagct cccgattggc tagtgaacct
    27841 actgctaaaa aaaccgcaag aaataaagac tcaataccga cccgtagcaa aaacagggac
    27901 agcaaaaaaa ttagatatga ttgttcaagg cgctggcgaa ggtagtagaa acgatcattt
    27961 aactaagtta tgcggttggt tactatggca cggtgtagat aatcaaactt tagcagaatt
    28021 gatttataca gcaaatgcgt acaatgaacc accgctaaaa gataaagaag tgaatcaaat
    28081 tattcgatcg atgattaaaa aagatttgag ggggaaagct catggctcat aagcataaat
    28141 tagatagtat tttagacttt cccgaagcca gcgagcgtga agacaatatc attgaattga
    28201 aaacctggat gtcacgatta agatgcaata aagacgatca gattaaaagt aacagtgttg
    28261 tgaatgcaga attgatttta accaatgata gcaatttagc tggaacaatt gcttataacg
    28321 aatttagcgg atacattcac ctattaaaag attctccttg gatcaatcgt accgctggag
    28381 aatgggaaga cagttttgag gatgccttaa cggcatatat tgaagaaaac tacaatgtag
    28441 tttttgatga caacaaaata cacaaagcag tagtaaatgt ggctagaaag aatgttttca
    28501 acccagtaaa agaacgtatc gaaaaagtta aatgggatca acagcctaga ctggaaacac
    28561 tgtttattga ccttttaggg gtagacgaca acctttacac acgagaagta acaaaacgtt
    28621 ggatagtcgg atcagtcgca cgtatctata aaccaggaat aaagtttgag atcgttccag
    28681 ttttggatgg accgcaaggg attggtaaga gtaccgttcc agcattactt tacacggatg
    28741 attttttcac cgattcatta gattcgctag gtgaaaagaa agatgattat atgcagcttc
    28801 aaggcaatgt aattattgaa cttggagaat tatcttcaat gaacaaaact aaaattgaac
    28861 agataaaaaa cttcatcagt gccaagatcg ataaaattag acctccttat ggtcggaatg
    28921 tcatagcttg ggcaaggcaa tgtgtattta ttggaacgtc gaatgatggg cagtatttga
    28981 aagatgatac aggaaaccgc cgtttttatc ctttgccttg taagaataag ccaaagatga
    29041 atccgtttaa aacaaaggat gaatattttc ttcaagtgtt agctgaagca aaggtgcttt
    29101 ttgataaagg acaacgaatc tattttagtc ccgatgaaga taaagaagtc ttagaaattg
    29161 caaaagacta tcaagaggat gccaagatgg agaatccaat taaagaagca atcactaaat
    29221 atttggaaat ggaaatcccg tataaatggg aagaagctcc agcgtgggcg agacgttctt
    29281 attatcagaa ctatcctgac agtaaatgta atgagaaaaa tttacagcat ttctataatg
    29341 gagctactat gcaacaagag ttttatttag ttgatagcgt actcaccgct gatattttgg
    29401 aagctgtttt tgataaacaa gcaaaggatt tattgaatgg tcgatcagat gcagaaacta
    29461 aaaagattgc cttgattatt agcagtattc ctggttggga acggaagcaa ctgccaaaaa
    29521 gaaataaacg acgaggattt tacaataagt caaatgccta tgaaaataaa aatcgagctg
    29581 gaaagtacaa aaagtaacgg gtagtaacgg gtcagtaaca cctagcccgt tactagttta
    29641 cccttactgc cccaagggat acagcaattt ggtaacgggg taacacctta tttccttaaa
    29701 acttttgaaa tagttagcta atatgctggg gaataatgtt actggatttt acccgttacc
    29761 tgttaccaaa acgctagaac ccttgtcccc gttgggattg agtggtaacg ggtacctgtt
    29821 acgtaggtgt taccacgtgt tacgaatcaa ggaggaaagt aaatgctcaa tattatttca
    29881 acaaacaaag caccgaactt tcaatataca gatgaaatgg atcggtttct aatgaacacg
    29941 ctggcattta gtgtgggatt ggtaacagaa gattattcca catttgatcc agaagtatta
    30001 aagataatgg aagaagaacc tgattggctg caggagtcag tagcgtggtg tcaatcgcta
    30061 gtagtgggat cattggtaga tagtggcaac tatgatgata caggtgagct aatggatgaa
    30121 tttaattgtc tgcttaacct atatgatcga gcgagacaac gagagcttac atcgaatgag
    30181 gataatttgt ttttaaacat ccatgataag ttcttggctt tgctactaac agatgatgaa
    30241 ttgataacta atttattgga ggtagaataa ttatgcatat ggaaagtatt agatacttcg
    30301 atggtgagaa acacattgag tattcaaaca ctcaagaaga tgtagacttt attagttttg
    30361 aggaagataa aatcactaaa gttaatttta aagacggaac gtgcttgaag atagtttcgc
    30421 cttatattga atacaaatca aaatgggaac aagatgattc tgacgaacga gatcgctttg
    30481 gttggtaatc tatggcagtt aaaaaacaat gtaatcatgc tggatgtaaa acattgattg
    30541 attataggca gaagtattgt gggaagcata aagcaaagca aacggcaatt aagcgtgaag
    30601 aaagaaagca aagcgaaggt aaatattttc aattctatca aagtagaaca tggcgaaagg
    30661 cttcatactt gtatcggtta aatcatccag tatgtgagga ttgcctagaa gaaggcttga
    30721 taaggaaagc tgatgttgta gatcacaaaa ttgaactaaa agatgattgg aatagacggt
    30781 tagatgaaac taattttcgt tctttatgcc atgcacacca taattccaag acagccaatg
    30841 aaagacaaag acgagaaaag agtacccttt gagttaaggg ggcatagtgt gaaacttact
    30901 gacaatcgat gcctactcat tttggtacaa ataaccgttg tgaaaaggca taagggtaat
    30961 tacaaataac gattacactt gtaatcacaa gtgaaaaagt atataatgaa agtaggagat
    31021 ttattgaaaa atacaattgt tattactgat aaaacaggga aacagcgcac cattgagtta
    31081 cctcattttg gtcaagtgac cattcaaatg cagaacggaa aaatcattta tatagataaa
    31141 ttagataaag aaaaattctg atcgaaaaac gaaggaatgt gagcttaatt gcttacgttc
    31201 ctttttcttt tgtctgaaag gaggacaaca tgggacaccc taaaatatta cacgatacaa
    31261 aaggcaatat atcaagtgaa gaaaaagcaa tccgtgtgga cgctagagaa gaattgttca
    31321 agcagcaacc attaataaat atcacgcccc ctgactggat ggcagcgagc gctagaagtg
    31381 aatggaatcg tatcgtacca acattaaaaa aagattatcc attgagtgaa gcagattatg
    31441 gttcattggt agcctattgt ttagcctttg ctcgaatgaa aacagccgaa gccgagataa
    31501 gaaaatcagg tacgtttatc acatgcgaaa acggagtaaa gaaagccaat ccagcggttc
    31561 gggttcaatc acaagctatg agtgatttga aaaaacaagc cacctcacta ggtatgacct
    31621 tagaatcacg atcaaaacta gctttgaaca aggttaaaaa tgatgaaccc gaagacccgt
    31681 tcaaagagtt gatgaattca tgaatgatta cattgagaaa gtcctatcag gtaagctgat
    31741 tgcacccaag aaaattatcc aagcgtgtga gcgtcatata agcgatttgg agcgttctaa
    31801 gtcagatagc ttcccttatg tgttcgatga agaacaagcc accaaagcga ttaaattcat
    31861 tgagttgcta ccatctacgg acggtaaagc aatcaagatg ttaggatttc aaaaattcat
    31921 tctaggcagt ctttatggct ggcgtactaa agaagggaat tacaggcgat tcaatcgagc
    31981 gtttaccagt atgagccgta agaatgggaa aacgtatatc gcaagtggca tggctgccaa
    32041 tgcgttgatt atggaacaag aaccagcaga agcaaggcaa gtattgtttg taagtaacgc
    32101 tttgaaacaa gctaaattgg gctatgatat gctgtctaat tcacttagaa acgtggtcaa
    32161 gtctagtaag tttttaagac cacaactgaa aattatgaac tctaagattc agcacttgcc
    32221 ctctaattcg ttcgctatgg cactggctag tgaaaccagc acgctagatg ggtttgcacc
    32281 aacaaccgca attcttgacg agtggcacga agcaaaaact cgtaaaacgt acaacgtcat
    32341 taagtcagga atgacccaac aaaagaacgg cttattgtgt gttattagta ccgctgggct
    32401 tgatttaaac gttcctatgt acgaggaata cttattgtta gatcgtgtgc taaaaggcga
    32461 agaacaagcc gacaggtact ttatagcgat atgggaattg gacgatcccg aagaaattca
    32521 cgatcaagag aaatggatca aagccaatcc gatttttgaa agtgaagaaa tcaaaaaagt
    32581 aatgattcca accattcaag atgatgtgaa ccttgctttg aaacaaaata accttaattc
    32641 tgtattggtg aaaaacttca atttatggag acaagcgagt gaggacagct atatgattgc
    32701 tgaagactgg caggcaaccg aagtagaacc acaagatatt acaggcaagc ccgtttatat
    32761 cggagtggat ttatctaaaa cagatgattt aactagcgtt tcatggatcg taccgctaga
    32821 taacggcgaa ctttattgtg attcacatag ctttgtagcc accaaatatg ggcttcaaga
    32881 caaagaaaag cgcgacggtt tgccttatcg agaactagaa aaagctggtg agtgttccat
    32941 tactcaatta gaaagtggaa tcgtggacta tgaccaagta tttcagttta ttcaagattt
    33001 gattcaagaa aatgatttgg aatgtatggg gatttgttat gacccgtata acgctaattc
    33061 gcttatcagt aaagctgaaa aagccaacta cccaatgtta gaagtaagac aaggaacgat
    33121 tactctaaac gttccgaccc gaacttttag agaacaagtt tatgaaggca acgttattca
    33181 caataaaaat acgattctca cccatgcagt gaacaacgct attttaaaaa cggataacaa
    33241 cggcattcag attaataaat caaagaacag taacaaaatt gatccaatag ctgcattaat
    33301 caatgcctat gtgtttgcaa tggattactt caccacaacg gaaggagcga aagcagacaa
    33361 tgaattttat acaagtgaag aattttcttt ctaattacat tcataccgtt cttttacttc
    33421 ttggattggt gtgtgtgttg gttgctatca ccttactaac aaatgtctac tatggcttgt
    33481 tagcgctggg catagtgctt attggggtag cggtcatgct aaatacagaa cagaagggag
    33541 gttaaaagat ggcatttttt aaagcgagac aaaatacaac gggagatcct ttcctacaac
    33601 atgtggtatc aatccaatcg gatgattaca ccaccagctt tacaagcgtt cgtgcattaa
    33661 gaaatagtga tgtgtttgca gccgttcgaa ttattgctag tgatattgct tcaagtccga
    33721 ttcaattggt caaaaacaat atgccacaag ctgataatga actggtgaag ttactaaacg
    33781 agaaacctaa ttcagaaatg gatggttggc atttaaagtt tgctttggcg gtcaatatgt
    33841 tgctaaacgg taatagcttt gcagagatta aacgcaacgg tgaaaaggta gaagaaattc
    33901 acttattacc taactcaagt gtaacggtta ctcaattaga taatggtacg ttgtcttatc
    33961 agattggtga taaaaaaaga cgtgtgaagt ctagcgatat tttgcacttc aaatatttca
    34021 cccaagatgg tttaacagga ttaccaccac tttatgcgtt acgtgatgaa ctgaaaattc
    34081 aacaagctgg caaccgcaca ttgcacaatt tctttactcg tggtgtcagt gggtcaggta
    34141 ttttgaaggt tcataagtca gatttagacg gatcagcaaa gaacgcaatt cgtgaaaagt
    34201 ttgaagaagc caacggttca agtagtggag ataatgctct tagaacgatc attcttgatg
    34261 aaacaatgga ctacaaaaca ttagaaataa acaccgatgt tttgaagctt atcaattcca
    34321 atgattggaa cacaaaacaa attgccaagg cgtttggtgt accgattgag cgtttaggcg
    34381 ttgaaaatga acattctagc accgttcaaa gtaatctcca gtatatccaa agtacgctga
    34441 ttcattactt taatgtgttt gtgagtgaat tagatacgaa acttgaaacc aatatccgct
    34501 ttaattccga tcagttacta gagactgatc ctgaaacaaa agtaaaaaat gtattggatc
    34561 aggtcaaagg gtcacttctc acgattaatg aggggcggtc gaaaatgggg ctacccccca
    34621 tggatggtgg cgatcgttta ctagcaagtt tgaactttac gtatttagat acgttggaga
    34681 aatatcaatt aaaagaacag gaaggagtta caccagttga ataatgaaga agaaaaggaa
    34741 aaacggctga cagaagaagc tgagctaaca gccgattctc ccaaagtaga gaaagaaaat
    34801 gaaaaacaac caacagacgg caaaactatt tcaggctatg cgttgaaatt cgggcaaccg
    34861 tcaaaagatt taggcggctt tgtagaagtg attacacccg aagcattaaa agaggtggat
    34921 ttatcaaatg tgttcttatt atatggacac gattattcaa agccactagc tagtgtcaaa
    34981 gcaggtacgt tgaaattgaa tgtcgatgat acaggattac attttgaagc agagctaacc
    35041 gacacgacct attcaaatga tgtgtatgag aatatctcaa aaggggttgt tgattctatg
    35101 tcctttgggt tcgtgcttgg attagattca ttcgacaaaa aagaagatgg cacaattgaa
    35161 cgatcaatag ataaaatcaa agcacttaat gaaattagtg tcgtgaccgt tcccgcttat
    35221 gattcatcaa atgtccaagt aaataagcgt tcttacgaat cgtttatgag taacaaccaa
    35281 gcaaaacaaa caaacaatag cttagaatcc acttctaaag cacaaaagga gagtaataac
    35341 atggaaaaaa ctttaatcga taacgaaaaa acagaattgc gtgggtatga agaatatatc
    35401 cgttcacaag gcgaagtgcg tgatggagtc actactgtaa atgcagcggc agttgttccc
    35461 gaagaagtaa tcggtgaagt ctttgatttg aaacgttcaa attataactt agctcaatat
    35521 gcaacagtaa aaacagtatc aaatggacaa ggtaaatatc cagtagcaac taaccaacaa
    35581 gcagtgttag caacaaaagc tgaacttgct gaaattggtg atattgacgc tgaaatgttt
    35641 acttcagttg attataaagt agaaactcgt gctggtaaga ttgccttatc aaatgaggtt
    35701 gtggaagatt cagcagtgaa tattgtacaa gaggtcaaag atcagttagc gaaattggta
    35761 gaaaacactg acaataagca tatcatggat ttattaaaaa cattcactaa gaaaacggct
    35821 gctacgttgg acgatttgaa acaactatac aatgtggcat tagacccagc attaaataaa
    35881 atggtaattc taaaccaaag cggatattac cacctagata cattgaaaga ttcagatgga
    35941 cgttacattt tacaacccga tgtgacagca cctagtggta aatcattatt cggtatgcca
    36001 gtagtattga ttgcagatac attgtttgcc aatcctaaag ctggaacatt ccctatgatt
    36061 atgggggata ttgcacaatc tatctttgtt gctcgtagaa atcaagtaac gactcaatgg
    36121 gaaaaattcg attactactc acaaggactt gcagtgatcg ttcgcaacga ttataagaaa
    36181 attgatcaaa atgcttcagt gtatattgag tttacgccag ttgtaacacc aaaagtatag
    36241 aaaaatattg ggcggcggtt tatcccactg cctttttttc attaggagag ataaaatggt
    36301 aattttggat agcataaaaa aaagtatgcg gatcgatcac actattgatg atgactttat
    36361 tcaacaattg attgatacag caggcgaata tataaaaagt gctattgata gtagcgcaac
    36421 ggataaagat atggataatt atcagcaatt tgatttggcg gtgtcattac ttactcaaca
    36481 ttggtatttg aaccgtcaag aagccagcag cgaacggata ccagtaacgg tacaagcatt
    36541 agtacaacaa atgagaggtg cttattatgc caatcattaa gaatgtaaat gaattgactg
    36601 agagagtcaa gtttaaaaag acaaaacggg taaaagatga agatgggcag atggtggata
    36661 gtgaagaaac tgtatttgaa tgttgggcta atgtgcgttc acaaatgcta aaagacgtgc
    36721 ttgctagtgt aggtaccatt cttgaaggaa cgttgacgtt tattattcga tacgatcaag
    36781 attatgaact aacaaacgat atgaaagtag cttggaaaaa caagagttat aaaattattt
    36841 caatcaatga aggaacggcg tttaaagatt atacaacgat catagctaaa cagatttctt
    36901 agaatgatta cacttgtaaa cgttgtgggt atttagtata attaaggtag taaatgaagg
    36961 gttagctgcc tgaattttgc aagacttagc tagtcgaaat ctattgttgg actgaaaatt
    37021 gtaggtgtgg ttgcaaacat gctggactaa gaaagatgat agatgatgaa cgttcaattt
    37081 taatacaggc atgctaggac tgattggatg gttttggcaa gctgattgtg tagaaatcaa
    37141 agaacacgtc tgttgtggac gtgttttttt tgttataatt caaaagaggt gatttaatgg
    37201 acaaagcaga gtttacaaat ttttattgga agtattatct acatttagaa aatgagttta
    37261 ttaataccac agattttgtg atgctcgatg agaataattt caaaacattc tctattgaat
    37321 atcaaaaact actactagcg attggttcgg agtgtgagat tattttcaaa gaactttgcg
    37381 gatttgatag cagtagcaat aaaagcataa ctgattttaa agctgttatt aaatctagcg
    37441 aattaatgtg tttggataat ggtgttaggg ttatgaattc tttaacattg acttcactaa
    37501 aaccttttgg agatgggtgg cctgaagata caccagattg gtggaaaata tataacagag
    37561 taaaacatgg tcgttcttca aattataagg aagcaaattt ggaaaatgtg ttgtatgctt
    37621 tagcaagtct atatttatta gaggaatatt tgcataagaa agtaatacta gaaggtgaag
    37681 tagactttat aagtccagag tcaaatctat ttacgttatc ttgggaaaga aaacattcgt
    37741 cgatgcaaaa agtgacgttt gaaaaagtgg attcaaatta tgtagctcca ctcattgatt
    37801 ttaaagaaca agaagaaatt taaaaacccc cacggcttat tgaagttttt gtatgccata
    37861 ttgtatgcca tttggtttaa atcgcttgaa aaatagtgat agataactct aattcattta
    37921 gtcataaacg ctgttttgat aacctttata gttctttgat atgggtttct cattcatggc
    37981 ggaagaagaa gaatagaaca aaagaatgaa ttaataagat ataaaaaaag ggattgtgac
    38041 aaacgtcttg tcacaatccc tttttccaaa taaacgatgt tcaatcagct gcccctcggc
    38101 attcgctcga aaaacggcga aatatgaaga actattttgc catttgtttc tctaataatc
    38161 aggtcaaaac gttgacttca cagtctcttc cttaaacggt gttctcctgc caactcctcg
    38221 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
    38281 ctcggagctg aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa agcttgcatc
    38341 tgcggtaata agttcaacca aacgaaaatt atgaggagct atttttgttt ggctgttctt
    38401 attacttgat gcaggacagc tttttcacaa cctcttttcg tttagcacta aatttcctta
    38461 aaaagtagaa aagctttcga agcctcgttt gtttctttta ctgtataata aaagaaaaga
    38521 gtaacaatcg ctaaaagata taaacaagag aaaggaaata caagatgatt ggtctgattt
    38581 attttgtggt gatcgttctg gcgaacacag ttggcgctat ttccgggatg ggtggaggcg
    38641 tgttgatcaa accgattctt gatcttatcg gcgcccattc tgttgcaggg atttcttttt
    38701 actcaacagt tgcggtattc acgatgtcca ttgtttcgac tgtgcgacaa gtatcaagcg
    38761 ggaaatcact gaattggcag attgtcggtt gggtatctag tggagctgtt gttggaggag
    38821 tagtcggcaa cattgtattc gaagtttttt tacagttatt tgaaaacgag aagcatgtgc
    38881 agctgatcca aatttttttg acggtattga ccttggtttt tgcgttcttc tacaccaagc
    38941 atcatcagcc aaaatttcat ttgacgtcat ggacttggta ccttatatgt gggggggttc
    39001 tgggcttttt agctagtttt ttaggtattg gtggtggacc agtcaatgtt tctttgttga
    39061 tgctgatgtt tgcattgcca ataaaagaag caacactata ttctctatct acgattttct
    39121 tttctcaact ggcaaaattg gtaacgattg ctttaacaag tagctttatg cgctttgatt
    39181 tatctatgtt gttttatgtt atccccgctg ctgttattgg aggtttgtgg ggagcaagat
    39241 ttagcagggt tttgtctcca aaaaaagtga cctttatttt tcaagtaatt gtcctcgtgg
    39301 ttcttttgat caatctttac aatgctttcg tcatcttaca aacattagga gaatctagac
    39361 taacgcaagt caaaatcact gaaacatatt gttctaaatg aaaaaaggag tatactgtga
    39421 taaaaaaatt catttaaaaa aataaaaatt ggctggtgat ttggatgttg cgggaaatgc
    39481 gtttaggaga tgcagaagca ctacgagaaa tcaatgcgga acaattagga tatgatgtac
    39541 cattaaccgt tacggtgaga caaatgaaga aactgttgaa ggagccagaa aaaaattttt
    39601 tcttggtcta tgaagatcct acttctaaaa aagcagctgg ctatgtacaa gcggagtcct
    39661 atgaatcgat tttcagcgat ccgatgttca atattatggc acttgctgtg tctaaacatg
    39721 ctgaaaaaag aggaatcgga aaggccttga tgactggcgt agaagaagcg aaaagacgag
    39781 gaatatcagc aataagactt aattctgcag aatacagagt agaagcacac cgattttatg
    39841 aacgcatcgg ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc ctgtaaattt
    39901 ttatcaaaaa atacctggtc atcaagccga tacttgatga tcaggtattt tttttgacta
    39961 agaaagtaat tgcatcaaga aacctgagat gatcaaaacg atggcaccac caagccgatt
    40021 gcccatttga gcaaaagcaa taagttccat ccgattagct gcagataaga cagctacatt
    40081 tcctgtacca cccatcgagt tgttgcataa gccagcagta atagttgatt cgattggata
    40141 taaaccaaac aaacgaccga caaatcctga aactactgaa atcgcgataa cacttgtcaa
    40201 acataagaca acaaattgcc atgtcaaagc agaagccaat acatttagat tcaataacgc
    40261 aataccgata ccggcaagaa cagctgctgt caagtttttg acaatcaagt tgttgaacat
    40321 gatagctgca tcttcgtaat attctggtaa taggtttgcg attttataga taacaacgat
    40381 aatgatcatg aatgcgtaag catggacttt aggaacaaaa tagttacaga tttgtcctaa
    40441 gatgaagaaa gtcaatgata ccatcaaacc gacacctaat tgtggaatgg aaggttttac
    40501 atttctaggt tttccatcgc caaggtcgcc tttttccatt aatttaccat taccgttgaa
    40561 ttttggcatt gattgtcccg cacgagcaac taacgctgca ccaatgatag ctaggacatt
    40621 tgtcattgct gtcgcaggaa tcaggcggga aatgatagaa gaagcgtccg tacctaaaac
    40681 atttgcataa atcgttgata aaggtacagc tccggctcca acaccaccag ccattgcggg
    40741 caatgagata tataaaacgg aatttgcaaa accatttccg ataagcatac ctacgagtcc
    40801 tacggcaaaa aaggagacga ccattgaaat aagtgcaacg ggaataaaac gaacagaagc
    40861 tttcatcaat agattacggt tcataccaag gatgctacca gtaatcaagg cagcaatata
    40921 aaagtctaaa aagcccatat tgctgacaaa gttttctgtg gattttacta catactcagg
    40981 taagatccca aatgtagcaa tcgcggcaga ggcaaagatc gcaaaaacgg agccaccacc
    41041 taaataactt ttgacgatag ggatttttgt tccgataaaa tgaaataaat taccgaaaat
    41101 gaccaaaaca gcgattgggc caatcatatt cgtcggtaat tttcctaaag ccattgcaat
    41161 gatcaggaca aggaccatga tcaaataaag gggcaaactt acgccggtaa tttttgttga
    41221 ccaaaaccct tcttttttct tttcctcaac tgcagttgcg gttgttgttt tttgtgccat
    41281 ttttacagat cctttcttat tctgtatcgt atgaaaaaag ccaaagagaa gcggcaaaag
    41341 agcagtagtt ggctggctgc tgttttgccg cagtcgcttt tttcttttta gacgtcatac
    41401 aaagtttctg tatgttcttt tactataaca atcttaatct tctaaagatt tatattcagg
    41461 aacccattta gcagattcaa cagcttcttt gatatccgtg atttcttcac gattcaattt
    41521 ttgtgcaaca actgattttg cgacagtttc agcaatcgtt tgagagaatt cagtgatttt
    41581 tgctactgga ggcaagatag ctgctcctgg ttttgttaca tcaacgattc cgcctaatgc
    41641 tcgactagct tgagaaatga tttcagcatt tactcgtgta gctgttgaag caatcaaacc
    41701 aagccctaaa cctgggtaca tcaacgcatt atttgcttga ccgatttggt aagttacacc
    41761 gttatattcc acatcagcag cagggatacc tgttccaact aaagcttttc cgtccgtcca
    41821 ttcaatcaaa tcttttgctt ttgcttctgc caattttgtt ggattagaca aaggcatgat
    41881 gattggacgt ggggtatgag ccgccatttc tttcacgatt tcttcagtga atgctccagg
    41941 ttgggtagaa gtaccgatca tgatcgttgg atggattgct tttacaactg cttctaagtt
    42001 tgtcaattca tcagcattag tgaattctga gcgtttacga gcgaatggga tttgaccagg
    42061 agtcaatcct tttgtatctt caaacaataa accctgttta tctacttgat agaaatgttg
    42121 gcgtgcttct tcttcaggta caccttgacg gatcatttca tctaaaagga tgtttgcgat
    42181 acctacacca gcagtacgag caccaaatgt caagatcgtt tgatctttca attgctcacc
    42241 tgatatattc aatccaccta atacaccagc tagtacaacg attcctgtgc cttggatatc
    42301 atcattgaaa gtagtgattt tatcttcgta tttttctaag atcgttgcag catttcctcg
    42361 tccaaaatct tcccaatgta ataacaattc tgggaataat tcagtagctg attgaacgaa
    42421 ttgatcaatg aattcgtagt aagtttcgcc ttctacacga gcatgacgat tccctaagta
    42481 taatggatta ttcaataatt cttcgttatt agtaccagcg tcgattgata caggcaagac
    42541 ttgtgcagga ttgataccag ccgcagcagt gtagaccatc aatttaccga tagcgatgtc
    42601 aacaccgttc acaccccagt cacccatacc taagattcct tctgcatctg tcacaacgat
    42661 caaacgaatg tcgcgtccat ctgctgcgtt tttcaaactt gctttgatat tttctggttc
    42721 gtcgattgaa agaaatgcag catcttgtgg tttcaagaaa atttcgttgt attgttcgat
    42781 tgaatccgca acgacaggat cataaacgac aggcatgaat tcaactaaat gttcgcccat
    42841 caatttataa aacaatgtcc ggttcgtatt gaatagattc attaagaaga tacgtttttc
    42901 caaatcggtc tgtttgctta agtattgacc gtaagcttgg acagattgtt gttccaatgt
    42961 ttggacagta cttggaagca taccagtgat tccgtatttt gcccgttctt cttttgtaaa
    43021 agctgttcct ttatttagaa aaggattatt taataagttt agccctgtaa gcatgaatga
    43081 ttcctcctaa gttttaatac aaacgaagta taggagtcag atgaggatat agtcaaaaag
    43141 agcaggtata ataaatttta tttatatgag taatagagtc aaaagaaatc aaaatagcgg
    43201 tatttgttct ttttatatca acaatccttg cttgattact atgcgtatct cttatatgct
    43261 aaagaaagaa aagtagaaga ataaaggata tgttagatag ataaatagaa tctctgaaga
    43321 ataaatcacg tgatcgtgac gataaaatac acttacgtca ggtgataaaa aatacataag
    43381 tagaaaacta agaggaggaa cagaaaatga aagaagttca attgatcaga aaaagcgaac
    43441 tatcagaagg tggttgcaat gcatgcggcg tagtagaagc aacaagctat acattaaagc
    43501 ttggtgccaa caaagcgata atctctgagt tgacagtagg cggtcttgtg gacagtctgg
    43561 cgcttgctga aggttttatt ggagaagata tttatgagat gttttcagaa atccgtcagt
    43621 taaaaaaagg ggaaaactgc atagaggttc atcatgagag cccgaatgtg agattcaaac
    43681 gtggtgataa tgagatgatc ttcaataatc atgtgtccga tcacaccgag ctatatggaa
    43741 tcgtgaatca aatactaact gagctttttg gattgggacc ttatgcgttc aaggaagaaa
    43801 acggtaatcc aaaattaaac gaagaatggc aagaaacgat tgaaatacaa agaaacaacc
    43861 cgcacttatt tcaataggca tccacagtat ttcgtaaaag ggactttgac acgacttttg
    43921 tcaaagtccc atcgtttttt atctatttgt gttctaaaca tttctttcag agtagtaaca
    43981 actggaatga ctgaaatgta aacagattat ttgcctgtat caatacggaa gagtaatata
    44041 aagtgtgtaa acgctatcat attattcatt caatttttta tagagggaag gcattgaaat
    44101 ggaaaaaacg tatcaagcag gtacaaatga aggaatagtg gattttatca atatggaaga
    44161 tttagaaata gccgcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg
    44221 tgctggcgac ttatttactt atcaagaaaa tgaacgggca ttcaatcatc agctgattat
    44281 tcctcatgta ttaaaagatg tagaattacc cgatacaact acacattttg acaaagaaac
    44341 gctgactgca ccaatcatca tggcaccagt tgctgctcat ggcttagctc atgtgaaggc
    44401 ggagaaagct tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta
    44461 cgcttcttgt acattagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt
    44521 tcaattttat atgagcaaag atgatgggat caatttggat attcttgaag tggccaagcg
    44581 taatggagca aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac
    44641 agaccgcaga aatgggttta ccttcccact gccaatgccg atcgttcagg cttatcaatc
    44701 aggagtaggc caaacgatgg atgctgtgta caaatcttcc aaacagaagc ttagtccaaa
    44761 agatgtagaa tttattgctg ctcattctga tttgccagtc tatgtgaaag gtgtccagtc
    44821 agaagaagat gtctatcgtt cattagaatc tggtgctggc ggaatctggg tatctaatca
    44881 tggtggccgt caactagatg gtggtccagc agcgtttgat tcgttgcaat atgtggcaga
    44941 agcagtagat aaacgtgtac caatcgtgtt tgacagcggt gtacgacgcg gacagcatgt
    45001 tttcaaagca atcgcatcag gtgcagatct tgttgctatt ggcagacccg ttatttatgg
    45061 attgtcttta ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt
    45121 ggaaatggtc atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt
    45181 gcgtgagaat cgttttattt aagaaacaaa tgacgaaaaa aataggagtg cggcagatga
    45241 tgccgtactc ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa
    45301 aataaaattg aaatattttt tcatacttaa taaaaaagga agaatattca tgtaaacgat
    45361 tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt
    45421 catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat
    45481 ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct
    45541 tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg
    45601 aaccaacaaa taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag
    45661 cagatccgga aaatccagca actggccaga caggtccttt aacactggat gtagttcccg
    45721 aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt
    45781 caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc
    45841 aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt
    45901 tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat
    45961 ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa
    46021 tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa
    46081 atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga
    46141 acctcgtggc tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa
    46201 tactataggt gaaggatttt ttaccgcagt ttttgttgaa aggatgtggt ggacaatgaa
    46261 tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc
    46321 gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga
    46381 gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca
    46441 gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt
    46501 caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt
    46561 agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa
    46621 tgtatcagca catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt
    46681 taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa
    46741 tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca
    46801 agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt
    46861 caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa
    46921 tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa
    46981 tgcattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa
    47041 agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg
    47101 gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc
    47161 cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt
    47221 tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga
    47281 aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa
    47341 gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga
    47401 ttacatttta tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa
    47461 aacagcagac acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga
    47521 cgaatgaaca aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg
    47581 cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa
    47641 ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt
    47701 tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg
    47761 tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc
    47821 caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca
    47881 aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca
    47941 aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat
    48001 ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac
    48061 ttgtatattg ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc
    48121 cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt
    48181 gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca
    48241 gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt
    48301 ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt
    48361 tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca
    48421 ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct
    48481 gcaagtgcag agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa
    48541 tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc
    48601 tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca
    48661 tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca
    48721 atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac
    48781 agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag
    48841 tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag
    48901 ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag
    48961 ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca
    49021 gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca
    49081 ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt
    49141 actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact
    49201 cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac
    49261 cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa
    49321 gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg
    49381 atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact
    49441 atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata
    49501 ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa
    49561 tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt
    49621 gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa
    49681 gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg
    49741 ttgaagacaa gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg
    49801 cttccaagca cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat
    49861 caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg
    49921 attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt
    49981 ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct
    50041 caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc
    50101 taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat
    50161 gagaaggaaa cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt
    50221 cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga
    50281 aaaaggagag gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt
    50341 gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt
    50401 tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta
    50461 ttatcattgt tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa
    50521 tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc
    50581 tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg
    50641 gcggctgacc ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg
    50701 acgtccctat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga
    50761 tctgctgcaa aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat
    50821 atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag
    50881 caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt
    50941 gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta
    51001 tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt
    51061 gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc
    51121 gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt
    51181 gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg
    51241 ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg
    51301 tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa
    51361 tgcccgcttt gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt
    51421 agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt
    51481 gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga
    51541 gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa
    51601 gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa
    51661 ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa
    51721 atcgtgatca ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt
    51781 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
    51841 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
    51901 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
    51961 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
    52021 acctatgata aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt
    52081 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
    52141 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca
    52201 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
    52261 tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
    52321 ataaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
    52381 tccgtacaac ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact
    52441 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
    52501 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
    52561 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
    52621 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
    52681 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
    52741 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
    52801 caatacgacg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
    52861 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
    52921 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
    52981 ctgtctgaat acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
    53041 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
    53101 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
    53161 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
    53221 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
    53281 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
    53341 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
    53401 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
    53461 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg
    53521 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
    53581 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
    53641 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag
    53701 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
    53761 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa
    53821 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
    53881 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
    53941 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
    54001 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
    54061 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
    54121 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
    54181 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
    54241 tgttagtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
    54301 cgttgaaggc aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt
    54361 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
    54421 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
    54481 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
    54541 agaattcagt aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt
    54601 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
    54661 agataagatt aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg
    54721 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
    54781 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
    54841 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
    54901 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
    54961 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
    55021 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
    55081 tgtttatgtc caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga
    55141 tcacccagcc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
    55201 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
    55261 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
    55321 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
    55381 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
    55441 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
    55501 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
    55561 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
    55621 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag
    55681 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    55741 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    55801 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    55861 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    55921 cttttacagc cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga
    55981 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    56041 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    56101 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    56161 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    56221 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat
    56281 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    56341 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    56401 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    56461 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    56521 acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    56581 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    56641 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    56701 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    56761 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    56821 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    56881 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    56941 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt
    57001 ttaaggaaga ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa
    57061 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    57121 cgtttaagaa aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga
    57181 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac
    57241 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
    57301 agtagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
    57361 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    57421 gacggaaggg tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca
    57481 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    57541 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    57601 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    57661 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    57721 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    57781 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    57841 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    57901 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    57961 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    58021 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    58081 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    58141 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    58201 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    58261 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    58321 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    58381 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    58441 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    58501 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    58561 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    58621 aaagaatatt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    58681 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    58741 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    58801 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    58861 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
    58921 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
    58981 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
    59041 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
    59101 cattttttga tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt
    59161 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
    59221 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
    59281 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
    59341 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
    59401 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
    59461 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
    59521 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
    59581 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
    59641 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
    59701 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
    59761 tacctaaaat tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga
    59821 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
    59881 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
    59941 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
    60001 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
    60061 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
    60121 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
    60181 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg
    60241 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
    60301 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
    60361 tcatccacaa tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
    60421 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
    60481 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
    60541 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
    60601 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
    60661 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
    60721 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
    60781 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
    60841 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
    60901 caacaacgca ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg
    60961 ccagcatgga gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc
    61021 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
    61081 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
    61141 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
    61201 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
    61261 aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa
    61321 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
    61381 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
    61441 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
    61501 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
    61561 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
    61621 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
    61681 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
    61741 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
    61801 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
    61861 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    61921 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    61981 ctagtcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    62041 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    62101 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    62161 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    62221 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    62281 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    62341 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    62401 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    62461 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    62521 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    62581 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    62641 attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    62701 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    62761 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    62821 taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac
    62881 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    62941 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    63001 atttgggaca atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    63061 tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    63121 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    63181 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    63241 tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt
    63301 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    63361 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    63421 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    63481 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga
    63541 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    63601 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    63661 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    63721 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    63781 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag
    63841 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    63901 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg
    63961 ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc
    64021 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    64081 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    64141 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    64201 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    64261 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    64321 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    64381 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    64441 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    64501 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    64561 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    64621 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    64681 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    64741 gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa
    64801 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    64861 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    64921 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    64981 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    65041 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    65101 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    65161 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    65221 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    65281 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    65341 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    65401 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    65461 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    65521 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    65581 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    65641 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    65701 caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa
    65761 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    65821 ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca
    65881 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    65941 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    66001 ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    66061 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    66121 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    66181 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    66241 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg
    66301 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    66361 attccattct tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg
    66421 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    66481 tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca
    66541 ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa
    66601 atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat
    66661 ttattttact atattggata tatattcatt gaaaatagtg tataacaact ttataatatt
    66721 gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa
    66781 attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
    66841 tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca
    66901 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca
    66961 aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt
    67021 aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct
    67081 tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga
    67141 atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga
    67201 ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac
    67261 gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat
    67321 ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt
    67381 agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
    67441 atgtttactg acacttttac ggattgcagc acgctataat tatttatttg aattagagaa
    67501 agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa
    67561 tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga
    67621 aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg
    67681 tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc
    67741 aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca
    67801 gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta
    67861 ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg
    67921 aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa
    67981 gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt
    68041 ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac
    68101 cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat
    68161 ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc
    68221 ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac
    68281 aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga
    68341 gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta
    68401 tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat
    68461 cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac
    68521 aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa
    68581 aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga
    68641 cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt
    68701 gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca
    68761 cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    68821 acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa
    68881 gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa
    68941 ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc
    69001 gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc
    69061 gaggatttta tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa
    69121 agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac
    69181 aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg
    69241 agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa
    69301 tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca
    69361 gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg
    69421 gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg
    69481 actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc
    69541 tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc
    69601 cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat
    69661 cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt
    69721 attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt
    69781 tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa
    69841 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    69901 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    69961 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    70021 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    70081 gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa
    70141 gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa
    70201 gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    70261 gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc
    70321 ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    70381 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    70441 cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt
    70501 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa
    70561 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    70621 ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa
    70681 tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga
    70741 agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    70801 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    70861 aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat
    70921 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt
    70981 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    71041 tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa
    71101 gaagataacg acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga
    71161 agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac
    71221 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt
    71281 gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga
    71341 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga
    71401 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat
    71461 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct
    71521 gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg
    71581 tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag
    71641 ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat
    71701 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    71761 acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt
    71821 gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt
    71881 ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat
    71941 cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg
    72001 tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag
    72061 ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat
    72121 gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc
    72181 tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt
    72241 gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct
    72301 gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga
    72361 ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt
    72421 attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat
    72481 gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct
    72541 gattctgcaa ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat
    72601 ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc
    72661 tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc
    72721 aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    72781 gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc
    72841 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac
    72901 gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa
    72961 aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa
    73021 atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga
    73081 caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact
    73141 gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca
    73201 ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct
    73261 cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    73321 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    73381 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    73441 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    73501 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    73561 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    73621 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    73681 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc
    73741 tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    73801 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    73861 ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    73921 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    73981 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    74041 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    74101 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    74161 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    74221 tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    74281 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    74341 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    74401 tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    74461 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    74521 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    74581 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    74641 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    74701 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    74761 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    74821 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    74881 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    74941 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    75001 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    75061 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    75121 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    75181 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    75241 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    75301 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    75361 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    75421 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    75481 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    75541 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    75601 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    75661 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    75721 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    75781 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    75841 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    75901 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    75961 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    76021 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    76081 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    76141 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    76201 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    76261 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    76321 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    76381 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    76441 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    76501 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    76561 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    76621 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    76681 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    76741 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    76801 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    76861 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    76921 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    76981 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    77041 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    77101 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    77161 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    77221 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    77281 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    77341 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    77401 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    77461 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    77521 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    77581 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    77641 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    77701 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    77761 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    77821 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    77881 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    77941 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    78001 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    78061 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    78121 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    78181 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    78241 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    78301 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    78361 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    78421 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    78481 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    78541 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    78601 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    78661 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    78721 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    78781 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    78841 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    78901 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    78961 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg
    79021 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    79081 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    79141 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    79201 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    79261 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    79321 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    79381 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    79441 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    79501 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    79561 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    79621 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    79681 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    79741 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    79801 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    79861 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    79921 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    79981 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    80041 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    80101 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    80161 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    80221 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    80281 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    80341 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    80401 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    80461 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    80521 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    80581 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    80641 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    80701 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    80761 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    80821 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    80881 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    80941 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    81001 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    81061 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    81121 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    81181 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    81241 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    81301 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    81361 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    81421 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    81481 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    81541 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    81601 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    81661 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    81721 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    81781 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    81841 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    81901 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    81961 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    82021 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    82081 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    82141 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    82201 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    82261 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    82321 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    82381 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    82441 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    82501 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    82561 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    82621 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    82681 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    82741 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    82801 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    82861 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    82921 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    82981 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    83041 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    83101 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    83161 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    83221 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    83281 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    83341 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    83401 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    83461 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    83521 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    83581 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    83641 tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    83701 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    83761 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    83821 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    83881 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    83941 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    84001 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    84061 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    84121 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    84181 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    84241 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    84301 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    84361 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    84421 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    84481 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    84541 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    84601 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    84661 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    84721 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    84781 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    84841 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    84901 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    84961 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    85021 cgaatggaga tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg
    85081 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    85141 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    85201 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    85261 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    85321 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    85381 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    85441 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    85501 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    85561 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    85621 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    85681 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    85741 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    85801 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    85861 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    85921 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    85981 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    86041 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    86101 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    86161 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    86221 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    86281 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    86341 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    86401 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    86461 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    86521 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    86581 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    86641 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    86701 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    86761 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    86821 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    86881 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    86941 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    87001 atcgcttcat gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa
    87061 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    87121 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    87181 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    87241 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    87301 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga
    87361 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    87421 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    87481 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    87541 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    87601 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    87661 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    87721 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    87781 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    87841 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    87901 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    87961 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    88021 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga
    88081 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    88141 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    88201 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    88261 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    88321 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    88381 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    88441 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    88501 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    88561 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    88621 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    88681 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    88741 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    88801 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    88861 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    88921 caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa
    88981 cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt
    89041 actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc
    89101 gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag
    89161 gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa
    89221 gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc attagatagt
    89281 tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag
    89341 ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg
    89401 gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact
    89461 atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta
    89521 cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa
    89581 aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg
    89641 ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct
    89701 ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa
    89761 tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat
    89821 cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa
    89881 tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga
    89941 aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta
    90001 ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag
    90061 gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa
    90121 gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt
    90181 tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta
    90241 ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc
    90301 tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga
    90361 aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac
    90421 gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt
    90481 tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata
    90541 gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa
    90601 attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    90661 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    90721 gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa
    90781 tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc
    90841 tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa
    90901 aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg
    90961 aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg
    91021 gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa
    91081 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
    91141 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    91201 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
    91261 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    91321 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
    91381 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    91441 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    91501 taacaatatc tatacaggta caacaaaaaa ctgtccacca atttt
//
LOCUS       DAYYCM010000004        79503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000004 DAYYCM010000000
VERSION     DAYYCM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.004"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..958)
                     /locus_tag="ACHAY1_000275"
     CDS             complement(218..958)
                     /locus_tag="ACHAY1_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592307.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1088..2035)
                     /locus_tag="ACHAY1_000276"
     CDS             complement(1088..2035)
                     /locus_tag="ACHAY1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8592308.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2164..2928)
                     /locus_tag="ACHAY1_000277"
     CDS             complement(2164..2928)
                     /locus_tag="ACHAY1_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8592309.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2932..3321)
                     /locus_tag="ACHAY1_000278"
     CDS             complement(2932..3321)
                     /locus_tag="ACHAY1_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592310.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3456..3998
                     /locus_tag="ACHAY1_000279"
     CDS             3456..3998
                     /locus_tag="ACHAY1_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8592311.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4146..4778
                     /locus_tag="ACHAY1_000280"
     CDS             4146..4778
                     /locus_tag="ACHAY1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592312.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4912..5037)
                     /locus_tag="ACHAY1_000281"
     CDS             complement(4912..5037)
                     /locus_tag="ACHAY1_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8592313.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5171..6064)
                     /locus_tag="ACHAY1_000282"
     CDS             complement(5171..6064)
                     /locus_tag="ACHAY1_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8592314.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6246..7172)
                     /locus_tag="ACHAY1_000283"
     CDS             complement(6246..7172)
                     /locus_tag="ACHAY1_000283"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8592315.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7257..8018)
                     /gene="pflA"
                     /locus_tag="ACHAY1_000284"
     CDS             complement(7257..8018)
                     /gene="pflA"
                     /locus_tag="ACHAY1_000284"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8592316.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8262..10493)
                     /gene="pflB"
                     /locus_tag="ACHAY1_000285"
     CDS             complement(8262..10493)
                     /gene="pflB"
                     /locus_tag="ACHAY1_000285"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8592317.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10770..13220)
                     /gene="parC"
                     /locus_tag="ACHAY1_000286"
     CDS             complement(10770..13220)
                     /gene="parC"
                     /locus_tag="ACHAY1_000286"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8592318.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13232..15277)
                     /gene="parE"
                     /locus_tag="ACHAY1_000287"
     CDS             complement(13232..15277)
                     /gene="parE"
                     /locus_tag="ACHAY1_000287"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8592319.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15443..15877)
                     /locus_tag="ACHAY1_000288"
     CDS             complement(15443..15877)
                     /locus_tag="ACHAY1_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8592320.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            16004..16642
                     /gene="plsY"
                     /locus_tag="ACHAY1_000289"
     CDS             16004..16642
                     /gene="plsY"
                     /locus_tag="ACHAY1_000289"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8592321.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16774..17649)
                     /locus_tag="ACHAY1_000290"
     CDS             complement(16774..17649)
                     /locus_tag="ACHAY1_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8592322.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17731..18522)
                     /gene="codY"
                     /locus_tag="ACHAY1_000291"
     CDS             complement(17731..18522)
                     /gene="codY"
                     /locus_tag="ACHAY1_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8592323.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18540..19940)
                     /gene="hslU"
                     /locus_tag="ACHAY1_000292"
     CDS             complement(18540..19940)
                     /gene="hslU"
                     /locus_tag="ACHAY1_000292"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8592324.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19954..20502)
                     /gene="hslV"
                     /locus_tag="ACHAY1_000293"
     CDS             complement(19954..20502)
                     /gene="hslV"
                     /locus_tag="ACHAY1_000293"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8592325.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20539..21444)
                     /gene="xerC"
                     /locus_tag="ACHAY1_000294"
     CDS             complement(20539..21444)
                     /gene="xerC"
                     /locus_tag="ACHAY1_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8592326.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21642..23018)
                     /gene="trmFO"
                     /locus_tag="ACHAY1_000295"
     CDS             complement(21642..23018)
                     /gene="trmFO"
                     /locus_tag="ACHAY1_000295"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8592327.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22987..25065)
                     /gene="topA"
                     /locus_tag="ACHAY1_000296"
     CDS             complement(22987..25065)
                     /gene="topA"
                     /locus_tag="ACHAY1_000296"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8592328.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25187..26044)
                     /gene="dprA"
                     /locus_tag="ACHAY1_000297"
     CDS             complement(25187..26044)
                     /gene="dprA"
                     /locus_tag="ACHAY1_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8592329.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26100..26867)
                     /locus_tag="ACHAY1_000298"
     CDS             complement(26100..26867)
                     /locus_tag="ACHAY1_000298"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8592330.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26860..27720)
                     /gene="ylqF"
                     /locus_tag="ACHAY1_000299"
     CDS             complement(26860..27720)
                     /gene="ylqF"
                     /locus_tag="ACHAY1_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8592331.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLATELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27980..28378
                     /locus_tag="ACHAY1_000300"
     CDS             27980..28378
                     /locus_tag="ACHAY1_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8592332.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28615..29157
                     /locus_tag="ACHAY1_000301"
     CDS             28615..29157
                     /locus_tag="ACHAY1_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8592333.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29240..29791)
                     /gene="lepB"
                     /locus_tag="ACHAY1_000302"
     CDS             complement(29240..29791)
                     /gene="lepB"
                     /locus_tag="ACHAY1_000302"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8592334.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29943..30170)
                     /locus_tag="ACHAY1_000303"
     CDS             complement(29943..30170)
                     /locus_tag="ACHAY1_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8592335.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30167..30685)
                     /gene="msrA"
                     /locus_tag="ACHAY1_000304"
     CDS             complement(30167..30685)
                     /gene="msrA"
                     /locus_tag="ACHAY1_000304"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8592336.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30705..31418)
                     /locus_tag="ACHAY1_000305"
     CDS             complement(30705..31418)
                     /locus_tag="ACHAY1_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8592337.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31421..32317)
                     /locus_tag="ACHAY1_000306"
     CDS             complement(31421..32317)
                     /locus_tag="ACHAY1_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8592338.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32475..33317)
                     /locus_tag="ACHAY1_000307"
     CDS             complement(32475..33317)
                     /locus_tag="ACHAY1_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8592339.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33449..34102
                     /locus_tag="ACHAY1_000308"
     CDS             33449..34102
                     /locus_tag="ACHAY1_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8592340.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34165..34692)
                     /locus_tag="ACHAY1_000309"
     CDS             complement(34165..34692)
                     /locus_tag="ACHAY1_000309"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8592341.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34712..35659)
                     /locus_tag="ACHAY1_000310"
     CDS             complement(34712..35659)
                     /locus_tag="ACHAY1_000310"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8592342.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35671..37554)
                     /locus_tag="ACHAY1_000311"
     CDS             complement(35671..37554)
                     /locus_tag="ACHAY1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8592343.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37717..39795)
                     /locus_tag="ACHAY1_000312"
     CDS             complement(37717..39795)
                     /locus_tag="ACHAY1_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8592344.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39808..40083)
                     /locus_tag="ACHAY1_000313"
     CDS             complement(39808..40083)
                     /locus_tag="ACHAY1_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592345.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40445..40900)
                     /locus_tag="ACHAY1_000314"
     CDS             complement(40445..40900)
                     /locus_tag="ACHAY1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592346.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41054..42262)
                     /locus_tag="ACHAY1_000315"
     CDS             complement(41054..42262)
                     /locus_tag="ACHAY1_000315"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8592347.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42374..42706)
                     /locus_tag="ACHAY1_000316"
     CDS             complement(42374..42706)
                     /locus_tag="ACHAY1_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8592348.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42854..43729
                     /locus_tag="ACHAY1_000317"
     CDS             42854..43729
                     /locus_tag="ACHAY1_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8592349.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43811..44392)
                     /locus_tag="ACHAY1_000318"
     CDS             complement(43811..44392)
                     /locus_tag="ACHAY1_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8592350.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44398..45657)
                     /locus_tag="ACHAY1_000319"
     CDS             complement(44398..45657)
                     /locus_tag="ACHAY1_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8592351.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45659..46705)
                     /locus_tag="ACHAY1_000320"
     CDS             complement(45659..46705)
                     /locus_tag="ACHAY1_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592352.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46940..47215)
                     /locus_tag="ACHAY1_000321"
     CDS             complement(46940..47215)
                     /locus_tag="ACHAY1_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8592353.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47458..48768)
                     /gene="der"
                     /locus_tag="ACHAY1_000322"
     CDS             complement(47458..48768)
                     /gene="der"
                     /locus_tag="ACHAY1_000322"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8592354.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48950..50179)
                     /gene="rpsA"
                     /locus_tag="ACHAY1_000323"
     CDS             complement(48950..50179)
                     /gene="rpsA"
                     /locus_tag="ACHAY1_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8592355.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50314..50994)
                     /gene="cmk"
                     /locus_tag="ACHAY1_000324"
     CDS             complement(50314..50994)
                     /gene="cmk"
                     /locus_tag="ACHAY1_000324"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8592356.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51043..51678)
                     /locus_tag="ACHAY1_000325"
     CDS             complement(51043..51678)
                     /locus_tag="ACHAY1_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8592357.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51744..53147)
                     /locus_tag="ACHAY1_000326"
     CDS             complement(51744..53147)
                     /locus_tag="ACHAY1_000326"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8592358.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53134..54165)
                     /locus_tag="ACHAY1_000327"
     CDS             complement(53134..54165)
                     /locus_tag="ACHAY1_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592359.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54196..54417
                     /locus_tag="ACHAY1_000328"
     CDS             54196..54417
                     /locus_tag="ACHAY1_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8592360.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54582..55319)
                     /locus_tag="ACHAY1_000329"
     CDS             complement(54582..55319)
                     /locus_tag="ACHAY1_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8592361.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55472..56812)
                     /locus_tag="ACHAY1_000330"
     CDS             complement(55472..56812)
                     /locus_tag="ACHAY1_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8592362.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57049..58572)
                     /locus_tag="ACHAY1_000331"
     CDS             complement(57049..58572)
                     /locus_tag="ACHAY1_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8592363.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58950..59561)
                     /locus_tag="ACHAY1_000332"
     CDS             complement(58950..59561)
                     /locus_tag="ACHAY1_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8592364.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59684..59809)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59891..60607)
                     /locus_tag="ACHAY1_000333"
     CDS             complement(59891..60607)
                     /locus_tag="ACHAY1_000333"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8592365.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60613..61182)
                     /gene="scpB"
                     /locus_tag="ACHAY1_000334"
     CDS             complement(60613..61182)
                     /gene="scpB"
                     /locus_tag="ACHAY1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8592366.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61166..61936)
                     /locus_tag="ACHAY1_000335"
     CDS             complement(61166..61936)
                     /locus_tag="ACHAY1_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8592367.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61933..62304)
                     /locus_tag="ACHAY1_000336"
     CDS             complement(61933..62304)
                     /locus_tag="ACHAY1_000336"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592368.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62323..63210)
                     /gene="xerD"
                     /locus_tag="ACHAY1_000337"
     CDS             complement(62323..63210)
                     /gene="xerD"
                     /locus_tag="ACHAY1_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8592369.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63306..63779)
                     /locus_tag="ACHAY1_000338"
     CDS             complement(63306..63779)
                     /locus_tag="ACHAY1_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8592370.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63884..64747)
                     /locus_tag="ACHAY1_000339"
     CDS             complement(63884..64747)
                     /locus_tag="ACHAY1_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8592371.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64943..67042)
                     /locus_tag="ACHAY1_000340"
     CDS             complement(64943..67042)
                     /locus_tag="ACHAY1_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8592372.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67262..68371)
                     /gene="rpoD"
                     /locus_tag="ACHAY1_000341"
     CDS             complement(67262..68371)
                     /gene="rpoD"
                     /locus_tag="ACHAY1_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8592373.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68395..70236)
                     /gene="dnaG"
                     /locus_tag="ACHAY1_000342"
     CDS             complement(68395..70236)
                     /gene="dnaG"
                     /locus_tag="ACHAY1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8592374.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70418..72733)
                     /locus_tag="ACHAY1_000343"
     CDS             complement(70418..72733)
                     /locus_tag="ACHAY1_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8592375.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72918..73778)
                     /locus_tag="ACHAY1_000344"
     CDS             complement(72918..73778)
                     /locus_tag="ACHAY1_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8592376.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73837..74469)
                     /locus_tag="ACHAY1_000345"
                     /pseudo
     CDS             complement(73837..74469)
                     /locus_tag="ACHAY1_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            74636..75397
                     /locus_tag="ACHAY1_000346"
     CDS             74636..75397
                     /locus_tag="ACHAY1_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8592377.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75423..78014
                     /gene="mprF"
                     /locus_tag="ACHAY1_000347"
     CDS             75423..78014
                     /gene="mprF"
                     /locus_tag="ACHAY1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8592378.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78202..79389)
                     /gene="tuf"
                     /locus_tag="ACHAY1_000348"
     CDS             complement(78202..79389)
                     /gene="tuf"
                     /locus_tag="ACHAY1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8592379.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT        21733 a        16749 c        13619 g        27402 t
ORIGIN      
        1 atcttcttta taaaaaaata aactattttt gcaaacgatc tgcgaaaata gtttgttttt
       61 ttattttgcc attattttct tacttattgg gagaaaaaat tcgcttaaac tcaaaaaaat
      121 ttgaggatac gatataaact ttcggataga gtataaaata aggaaacgcc gcagcgtttc
      181 ctttccaact gtagccgaat ataaatttgt atatcactta tttaaaatag ctattttctc
      241 ccaaagagtt tccttgatca tcgatcaatc cttgtaatga gcgttctaag ataccatggc
      301 tttttttcga ttttttttct tctgactgct ttgtttcctc taacgttttt gttcgattga
      361 atgttcgtac agacggctca ccatttcttt tttcttgata cggcgtcggt tctgtatcga
      421 acaacagata agagtcatcc gtttttttca tcgattcgat taaatgatcc tcagaaattt
      481 tgctttctct agggtctggg ataatcgatg cgggtacata tttaggaaca aaataagaac
      541 gctcccgcac cttcgtgtca acagatgcgt ttcgtgtagt atgcttggac tctgtccttg
      601 tttcactttg tctgacagaa cgagaggaat caccaaaaag tttttttcgt cctgtcgatg
      661 tgatttctga ttggtaatgg ccggtataat ccggtaattt tcctttatgc ttttccaaat
      721 tttccttacg cggagctgac ttcaatccat ttgttgcacg acgcttgcga cggattttct
      781 cggtgttcgt gttcgtgaaa tgaaattctt tcatctgttc aaacggggac tgattctgtt
      841 cagtcaaaag atcttcttgt ccgtcaaata atactcgctt cttgtcgttg gttaatttta
      901 cgcctaactc atcatcgtac gcaggaaaac ggaaatgttt tcgtctgatt tctttcattc
      961 aaaccatcct tctcaaaaat ggaacttttc tcaataatag cacagattca ttgttttcag
     1021 tagcaatgcg ctgattattt taagaaaata gcgatgaatt tcataaatat atatatagta
     1081 ataagactta attcgttgtc tctctgataa ctagttcagg aaaaaaacga cgataacctt
     1141 gactcgcccc tttttcttct atttttttta acaacatttc tccacatact tcacccatac
     1201 gtataatatc ttgcttgatc gttgtcaatt taggtgaact gatttggtct aaaaataccc
     1261 catcaaatcc aatcacacca aattcttcgg gcaagtgtcc tccagcattt ataatccctc
     1321 ttactgctcc tagtgctagc cgatctgaac tacatacaaa acaagtttta tctgtaaaat
     1381 ctgcccagtt ttctgtcata tatctttcta cttctgtcga atgatttgga aaccgcaaga
     1441 ttttactagg aagatcaact tccttcatca cagataaata accagtttcc cttgaaattt
     1501 caaaaggctc gtccacttcg ataccgatat aaataatcgt ttgataatct ctttctaagg
     1561 catattgcgt tgcttttgct gtaccatatt cattatcgct gtctacataa tcatatcctt
     1621 cagaattttc cccaaacaag acaactggtt tttccaaacg gtcgatccaa tcgaagtctt
     1681 tttttctgac acctgtgatg atgtatccat cacaagaacc gacgtcgaag gcatcatttg
     1741 taactaattg gagagaataa tgcgctttac cgatccgttt agcgattccc atcagcaaat
     1801 tcatgtaata gggctcagtt gtgtcgattt cttccagtat aaataatttg atgatctgcg
     1861 accgattcgt gacaagtgct tttgcagcaa tatttggacg gtaatccagt tcagccattg
     1921 ctgagaaaac taactctttc aattcgtcag tcacctgttc cggatgattg atgactcgag
     1981 agaccgtcat ttttgaaaca ttggcacgtt ttgcaacatc tgctaaagtg ggcatgctct
     2041 cttcctttct aacatgttgt cattcttact atcaattata ccaaattttc ctaacagagt
     2101 ttattatagc ccaaaataaa aaacggaaaa tcctctatcg tcatgagtga ttttccgttt
     2161 caattattta tactttttta tcaaattttt tgctaaaaaa gtggaagctt cttccattag
     2221 ttgtatttgt accatatgtc gctgtgtttt tgaggaacga aaaacaattt cttttccgta
     2281 agacttatgc cgattttttt ctacaaatcg ttccgtttgt atataaggga ttttttcgtc
     2341 agcttgtcca tgccaaaaga acagaggacg tccagctaac ttttccggct gcaatgacaa
     2401 atcatagctc ttaacccatg aaatcaaatc aaaatagtct tttggtacga cataattata
     2461 ttccgatgcg cgccttacga tttcttttcc atattccaaa ggtgcagggg aacccatcaa
     2521 acacacgccc gctactatat gaggatgttt agccaacaag gcgcatgttg tcattccccc
     2581 catcgaaacc ccgccaactg caagctgatc atgtagaaga cctctttttt ctaaatgctt
     2641 gataagtgtg tcaaattcaa acagatttcc gtagatacta tcccaaaatg tataggatgg
     2701 aatagaagaa accggctgtt tgcgctcccc atgattcatc gcatctggca ggatcacacg
     2761 aatgccctgc tgcgcaattt ttcttccttg cgtcaaaact aactccttac ttgtctgcca
     2821 tccgtgataa tagatgacaa cgggaatacg ttcctctctt aattctttca gaacaacttc
     2881 taacactgga atatgatgga cagaacgttt caatataatt ggattcaatt gttactcacc
     2941 tattttcatt aaatttcctg atcgccatag caaccatgct ccaacagttg tctggaacag
     3001 gttcagtgta ccaaataata taaatgcagg actgaatggc tcgggaaata agcacagaga
     3061 acaaagtccc accactccta atgctagcga aaggagtgga taaatagaaa aattcaaaaa
     3121 actggcaacg ctaccgaaaa tattaccaat aaacagtatc aaaaagaaaa aaacgcttaa
     3181 aattaaacca tctgtcagcg acatctgaaa aacaatgttc tctacctcaa ttggctgttg
     3241 aatcgaatag acacctaagt atatactgag aaggagactt agcacgtggc tggcacaaaa
     3301 cacacgattg accgttttca ttttaatcac ctaacctatc tttataattc tattataatc
     3361 attttatcat ttttttgtat gtataaagtt tcaatttttc ttcacttacg attttttttg
     3421 atattgttgt tatataaatg aaaggagttt tacaaatgat tgatccaaaa atgttgggga
     3481 tgatcttcct cattaatttt gcttatatca ctctgaacac gttacgtttc atgttgacaa
     3541 tgaaaggcta tcgactgatt gctccacttg tcagtatggt cgagatcacg atttatatcg
     3601 tcggcttatc ccttgtttta gatcgtttag ataatccact gaatttattt gtctatgcgt
     3661 taggttacgc agtaggtatc agtgtcggaa ttttgattga agacaaattg gcattaggat
     3721 acatcatggt cacaacgatt ttgccagcaa atagcgcaga agataaaagt ttgccgggta
     3781 tactgagaga acacggttat ggtgtgacac agacttctgc aatggggctg gaaggtgagc
     3841 ggctagttct cgaaatactt tctcctagaa aaagcgaacg tgaactgtat cgcctgatca
     3901 aagaaatcga agagcgtgct tttatcattt cttatgagcc aaaatatatt tcaggtggtt
     3961 tctggactaa aaaagtgaga aaacgacaag gaaaataact cacaaactta aaaactgtct
     4021 aatatctaaa ttaaatttta aatgttagac agtttttatt tattattttg attaaaaaac
     4081 atcgacatat actcatcaat ttgttattat actttatata cttatcaatt taatttggag
     4141 atattatgga aattcaacgt agaaccacta attttttaca tttaggctct ttttttgcaa
     4201 ctttgacgat cttatttttt ctatgttatt cataccttaa gacctcacat gcgccttcta
     4261 gtgcattagg ctggttgctt ttggatcatt ctgatatcac agaagccgaa gaaaatccgt
     4321 tttttttgat tttgtcttct ctttgcggct tactgttttc tgtttatttc ggtgcctatt
     4381 tttttcatcg acatcctggt acgttaaacg aagcttcaag cgtattgatt gcatttaaaa
     4441 cgttttcttt tttagcaatt cttttttatt cgtttaatca caatacgctt ttcttcttta
     4501 tcatgaatgg tttagtcgtg attttatcca tttttactta ttttattttt tcactcattt
     4561 ttagccaatt gaaatgtccg ctcttctttc gattagttcc tgtcacctgt tttatttaca
     4621 gcttgttttc cttttttgtc cttgctttaa tgccactgac caacccttcg acgatccaag
     4681 tcgcaggaaa tctgttggat atgctcttca tcgtcagtct aggtgtattt atgtggcaaa
     4741 gaaaaaacaa acaaaataaa aagcattttg aagcctgact attacgactt cataaatgct
     4801 tttttagttt taaacacttc aatctaaaag aataacccaa gattggataa attattaatg
     4861 actcgccatt cgtttcttag tgattctgga ttatcaaatg gacttcctaa tctaatcagc
     4921 tagttcttcc cataaaatgc catacttgac tgaatcataa tattgattat ttaaaaatct
     4981 aacttttctt atcactactt cctttgtcat tccacatttc tcaccatttt tttgcattcc
     5041 tttgtttcca gaccatgtag tgaatctaat aggtggtaga taggcaaaat actcaaataa
     5101 ttcttttaac cataaaggaa gagaaaggct cctcctgcaa gagtcagtat aatttttgct
     5161 tactgctctc ttaggtttta ctcactaaaa ctgttccata ttctttgata taagaaaatg
     5221 catgcggtat aaatttcttg ctcagctcag aaaagatttg tcgtttaaga aaggccattt
     5281 cctccattgt caatgaagga tccaatgttg gtaattcaat ctcaaaattg tttttttcga
     5341 caataggtaa ttctaatgtg aatgattctt cttcctttat agtattcgtc acaagtagag
     5401 actgatggaa aatactaggc cggcgatttt tatatttatt cttacgtatc ctgtcaacga
     5461 taacttctat ttgatcaaaa aagtacgatt catttacgtc tccaataagt tcggctaatt
     5521 ctttgaatgc cttcgttaca ggaagctgat tcttctgtac aaaaaagtaa ttagccaaag
     5581 taagcgggaa gtgttctata gaggaaagtg tgataaaacg gtacttctca tttcctgcag
     5641 ttgtcctcag caattctgtc tctcggctgt ttcttactcc ataaaaatac gcttctttcc
     5701 gttcatcttc gaaacaatac gcccaaataa cttctcccat cgctaatttt tgttcatcaa
     5761 cagagagtct cttcaattgt tcagctatgg tttcagcagc cgaagtggct tgttgcaagt
     5821 aatctttagg atcaaatatt ggaaagttca aatgatatct tcgttcttcc ctcttgatta
     5881 atcctgcatc gatcaataaa tcaagaaaat gggaaaattt attttgcgaa aattcttttt
     5941 tcaattctct tagaatcgtt tcttgttctc gatgttcata tagataatcg gtcaatgaag
     6001 aaaaaagcgg atcatcggtc agtttttgat aaaagttttc cttatttttt gaataatagt
     6061 tcatatatgt tctcttttct atccgtttta ttttacttaa aaggctttta cgtaaaaagg
     6121 aggaagaaca agagtcataa ttgactgtta ttctcctcca tttcttcggc ctttagtatg
     6181 ttctatgttt ctttttatat caatcgctta tccattcatt ttgaatgctt catactacca
     6241 tttgattagc taaatacagc cgtcaattga ggtacgactt gttttttacg tgaaacgaca
     6301 cctttaagca aagcacggtt gttggtcaaa gttgtttgga aagcttcttc tactttttct
     6361 tttcctttac cagcaacaag taattctgaa tcactgtcta agatgtttgt gacaactaat
     6421 acgaacagat cgtagccatt tgctttcatt tcatcttcca ttgctgcttc tagctctgct
     6481 tgtcgatcca tcacttcatt aaggtccact gtattgactt gagcgatacg aacgttggaa
     6541 ctgcccattg ggaaactttt tgcatccaaa tcaagaagaa ccgcagctga tttatcactt
     6601 agatttgttc ctgctttcaa catttcaagt ccataagtgt ttagatcgac ttcagcgatt
     6661 gctgcaagtt cttttgcagc tgcttcatct tcaggcgtac atgttggtga tttgaacaac
     6721 aatgtatctg aaatgatagc tgagagcatg attcctgcga tttctttagg aattgttact
     6781 tggttttctt tatatagttt taaaataatc gtacttgtac atccaactgg ttctgcacga
     6841 tagtacaatg gattagctgt ttcaaagttt gcaatacgat ggtggtctac tacagctaaa
     6901 atatttactt cgctgatatc tgaaatactt tgttggaatt cattatgatc cacaagcatg
     6961 acatctgcat tttctgcttt agctgactct actactcgtg gtgcagatag ctgaaagtgg
     7021 tctaatgcgt attgtgtttc ttcattaggt gtacctagag caactgcttc tgtttcttta
     7081 cctaattcat tttgcaagtg tgaaaacgca atggctgccc cgattgcatc tgtatctgga
     7141 ttttggtgac caaaaactaa aatttttgac atgtcattcg ctcctttgtg atttctccta
     7201 tttaataata gcaaaaaaac ggaaagaagc aatacttatc ttctttccga tgcgacctat
     7261 cgcgttaaat attttgtata ctcttctgta tgaagcaatc gtttagcatt ttcgacacgt
     7321 tcttttgtag gtggttcgat cccttctaat ggatatttca aaccaagtgt ttcccacttg
     7381 tattttccca tcgtatgata aggaagaatc tctactctgt ctacatttga caaggtttga
     7441 ataaagttgt ctagtcggat caaatattca tcataatcac ttcgctcagg aactagtaca
     7501 tggcgaatcc aaaccggttt gttgattttt gacaaatatt gagccatttc taaaatattt
     7561 tcattgtccc aatgcgttag ttttttatgc tcttcattat cgatgtgttt gatgtcaaat
     7621 aacagtaaat ctgtatactt catcaattct tgaaaacgtg aaaagaaagg ttcttcatac
     7681 gtaaaaggtt ttccgcatgt atccaatgta gtatgcatct tccgctcttt tgctttttta
     7741 aatagttcga tcaaaaaatc gatctgcaat agaggttctc caccactaac agtaatacca
     7801 cctttatctc cccagtaagg tctgaagcgt tctgctttat ctaaaagttc atctgctgtg
     7861 tattctttcc ctccgccaat attccaagta tctggattgt gacaaaattg gcaacgcatg
     7921 cggcatcctt gcataaacac gacaaagcgc agtccaggtc cgtctactga accgaaagtt
     7981 tcgatagaat gaacatagcc gattgttttt tcttccattt aagttcacat cttttctttg
     8041 atttcatttt aagtacaagt tagctgtcga agcgaagctt tctgaacaaa atttttcgca
     8101 attgccgctt cgttatgtgt aaaatttaca gggacggaac atacaagtcc tgcccctgta
     8161 aatcgtttct ttcaaataag catactgatt attcgatttt gatgcgatgg ctattcaacg
     8221 tcctttcgac gctaagtcga aaaacttttt tcacgaactt tttacattct gtcgtgtgaa
     8281 gttctcatca agacatctaa ttgttgttct cttgtcaagt cacggaattt aactgcataa
     8341 ccagataccc ggatcgtcaa gttcggatat ttcattggat tttccattgc atcacgcaat
     8401 aaatcattac tgaatacatt gacattcaaa tgataagctc cttttgagaa gtatccatct
     8461 aagacattac gcaagttttc gattcttgta tcttgttctt ttccaagacc tgaaggattg
     8521 attgtttggg tatttgaaat tccatccaat gcacagccgt aattcatttt agctgttgag
     8581 ttcaggctag ctaataaccc actttgttcc gcattgtaac ttggatttgc tcctggcgca
     8641 agtggttttc cagcttcacg gccatcagga gtatttcctg tatttttacc gtaaacaaca
     8701 tttgaagtaa tcgtcaataa tgaagttgtt ggttttgagt tgcggtaagt tttatgacgt
     8761 ttgatttgtc ccatgaagta ttccaagatc catgaaacta attcatctgc acggtcatca
     8821 tcatttccgt atttagggaa gtcgccatcc acttcaaaat caactgctat tcctttttca
     8881 ttacggattg cttttacttt tgcatatttg atcgctgaca agctgtctgc cgcatgagag
     8941 atcccagcaa tacctgtagc aaatgtgcgt tgcaagtcag aatccatcaa agccagctga
     9001 gctgcttcat aagaatattt atcatgcata taatgaatga tgttcaacgt attgacatat
     9061 agttctgcta accaatccat catattcatg aagttatgtt tcacttcttc aaagtctagt
     9121 atttctgatg tgattggacg atattctggt ccaacttggg cgccagtcac ttcatcgact
     9181 ccgccattga tggcatataa tagtgtttta gccaagttag cacgagcgcc aaagaattgc
     9241 atgtcttttc cgacaactgt tcctgataca cagcaagcaa tcgcacaatc atcagaaccc
     9301 cattgttcac gaagctgatt atcattttcg aattgaatcg aagaactttc gatagcgatt
     9361 ctgctagcat atgttctgaa tccttctgga aggtgttcag aatacaaaac agtcaagttt
     9421 ggttctggtg aaggtcccat atttgttaat gtatgaagca gacggaaatc attctttgta
     9481 actaatgaac gaccatctaa tcccatacct gcaatggata aagtagccca gatagggaat
     9541 cctgagaata attggttata ttcaggtgta cgcgcgaatt tgaccatacg taatttcatt
     9601 actaagtggt cgatcaattc ttgtgcttct ttttctgtga ttactccatt atccaaatca
     9661 cgttggatat agatatcaag gaatgctgag atacgtccga ttgacatcgc agcaccattt
     9721 tgtgatttga ttgctgctaa atatccgaag tatagccatt ggacagcttc ttttgcattt
     9781 gcagctggtt tagaaatatc atagccatat ttcgcagcca tttctttcat ttgcgctaat
     9841 gcacgatatt gttcagtgat ttcttcacgt aaacgaatca cttcttcact cattgtatga
     9901 taaccagtca tatcatgatc ttttttcttt tgttccatca aatagtcgat tccatacaat
     9961 gccacacgac gatagtcacc gatgatacgt ccgcgaccgt aagcgtctgg aagccctgta
    10021 atgattttgt ttttacgagc agcacgcatt tcagcagtat aagcgtcaaa tacaccttga
    10081 ttatgtgttt tgcgccagtc agtgaaaata tgtgtcaatt catcgtttgg tgtatagcca
    10141 ttgcttttta gcgcattgtt agccatattg attccgccga acggcatgaa tgcttgtttc
    10201 aaaggtttgt ctgtctgtag accgacgatt ttttccaaat ctttatccaa atagcctgca
    10261 tcgtgtgaag taatagttga tacgatttct gtgtccatat catagacacc gttacgctcg
    10321 aattggatat catttaactt ttgcaattca tcccataatt tttctgttgc ttctgttggg
    10381 ccttccaaaa agctgtcatc accgcgatat tcagtgtagt tattttgaat aaagtctcta
    10441 gtgtctacgc cttctttcca tttggtacct ttaaatccat tccattgctc cattgtatgg
    10501 acctcctaaa tttctgtttt atgtaacagt ttttacgtga ttagtataac acagcaccct
    10561 atctttgcaa gggttttctt gttttttatt taacaatttt tatgttttat ttacatatat
    10621 ttaaatcaac gttgacatac caacatttaa gcattgtgaa cactttcatc tttttttgca
    10681 aacgtataag gatttattaa aaatgaatga aaaagagacg ccacctgtat taatacagat
    10741 ggcgtctctt cattaaaata ctacaacagt tacgactcga tgaaaatctc aggagcatcg
    10801 ataacttcga tgacttctcc accttttttc tcatccatcg caaatgatcc attcgatgtt
    10861 cgatcaccta tagggaaatc agttgggtca atcgttaact gctgcccttt ttgattcatc
    10921 aaggttattt cttttgatct tccttccgac atatagataa tacggtgcgg attttttttc
    10981 agctcacgca tgaccgtcac tccccgcttc gcacgtgaag tttggctgat ttcttgagca
    11041 agcatgcgtt taacagctcc tcgctgagta acgattacga tcggcgtatc accttctgct
    11101 aacacaagca gcccgttgat aatcctatct ccatctttca aattcataga cttcactcca
    11161 gctgcctttg ctccaacaac gggaacatct gttaaaggat agcgcaaacc cattccttga
    11221 tgcgttacta agaaaacatc taagcctggc tgatcttctt tcagatacac agcaatcagt
    11281 tcatcttctt ccgcctttag cttcatacag ctgagcggac ggctcttata ggtgcgccat
    11341 ggttcgaact ctgtcattcg tgtttgcttg atcaatccat tcttgctgat gaatacaaat
    11401 gttttctctg cttgaatttt ttgatatggg aaaacagcta aaatcgattc gtctacagac
    11461 agattggaga ttgtttgaga aatgtgctca ccagcatctt tccatttcaa atcgggtagt
    11521 tcatggacag ggcggtagat cacatttgct ttatttgtga tcagaagaac atggtccaat
    11581 gtactcaatt cacctgagta tagcaagtag tcgccttctc gcatcccgat ttcttctggt
    11641 ttggaagcac tataagaacg aaggcttgtt cgcttcacat agccttctcg cgtcacactg
    11701 acaattacgt cttcttgtgc cactaagact tgcgtatcaa ttttgatttc ttcgatttcg
    11761 tcttcgattg tcgttagacg agcggaagaa taattttttt tcacttctcg caattctttt
    11821 ttcataacag agaaaagctc tttgtcattt gaaaggattt tattcaactc tgtgatttgc
    11881 gcaatcaagc tttcggattc cttttgaagc tctgtgatat ccgtgttcgt caaacggtat
    11941 aattgcaatg tgacaattgc ttctgcttgt tcttctgtaa attgaaagac gtcgactaaa
    12001 ttctttttgg cgtctttttt gtctttactt tcacgaatcg ttgcgattac ttcatccaga
    12061 atcgataaag ctttcatcaa tccttctacg atatgctgtc ttttttgagc tttggctaaa
    12121 tcgaattgac tacgttttgt tatgacttgc tttcgatgct cgatataact tcttaaaatg
    12181 tctttcagtc caacttgatg cggagtcata tggtcgatag cgaccatatt gaaattgtaa
    12241 ttgatctgta gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc attggcgtct
    12301 tttttcaatt cgacgacaat ttgcagacct gttctatcac tttcatctcg tacttcagct
    12361 attccatcga ttttcttatt caaacggatc tcatccattt ttttcaccaa ggtcgcttta
    12421 tttacttcat atgggatttc tgtgatcacg atttgctgtt tattaccctt cattgcctct
    12481 atctttgttt tcgaacgaag aataactttt cctcgtcctg tttcataagc ttttttgatt
    12541 tcatcttttc cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat atattccatc
    12601 aatttttcaa ggctcgcttg tggatgatcg atcatataga ttgtgccatc gatcacttca
    12661 gctaagttat gagtcgggat ttctgttgca tatccagcag atatacctgt cgatccattg
    12721 acgagaaggt ttgggtatcg agctggaagg actgttggtt ctttttctgt atcatcaaaa
    12781 ttccacacga gatcgactgt ttctttctcg atatctgcta gcatctctcc acttaacttg
    12841 gataatctgg cttcagtata acgcatcgca gcaggtggat caccgtccat actcccgtta
    12901 ttcccgtgca tttcgatcag tacttcccgc aacttccagt cttggctcat gcggaccatc
    12961 gcttcataaa tactgatgtc accatgagga tgataattcc ccatgatatt tccgacagat
    13021 tttgctgatt tacggaatcc tttgtcatac gtattgccat ctttgttcat ggcaaataat
    13081 atccggcgtt gcactggctt caatccatca cggatatcag gcagtgcacg ttcttggata
    13141 atatatttgg aataacgtcc gaagcggtca cccatcactt cttctaaagt taattcttgt
    13201 acttcttgtc gattttccat caggtgacct cctattctac ttcaaataac tgattgtttt
    13261 catttttatc tgctcgttct tcatctaata aatcattaga aactgaagga gagatttcag
    13321 tatcctcttt tttatctaag atactgccat cttcttcgag cgtaaattgc acgtgatttt
    13381 cgatccattt tcttcgaggt tctactttgt cacccatcag tgtggttact cgacgttcag
    13441 cttgcgcagc atcatcgata cggacgcgga tcaacgtacg ggaagtcgga tccatcgttg
    13501 tttcccacaa ttgttccgca ttcatctctc caagtccttt atatcgctgc agcatatatc
    13561 ctttgccgac ttttttaatc attgctgcca attcgtcatc tgtccacgca tattcaatga
    13621 cttgcttctt tccttgtcct ttggatactt tatataaagg cggtaacgcg atatatactt
    13681 tcccagcttc aatcagcggt ttcatataac gataaaagaa agtaagcagt aatacttgaa
    13741 tatgggcgcc gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa ttacagtctt
    13801 cgatagaaaa ctcaggaccg actcctgcac caatcgtata gatcatcgta ttgatttctt
    13861 catttttcaa gatatcttgc attttagctt tttcggtatt gatgactttt cctcgtaaag
    13921 gtagaattgc ttggaacttt ctgtctcttc cttgtttagc agatccgcca gcagaatccc
    13981 cttcgactaa atacaattca tttttcttag ggtttcggga ttgtgcgggt gtcaattttc
    14041 ctgaaagaag agattcgcct tttttacgct tcttcccatt tcggctttct tctctggctt
    14101 tacgtgctgc ttctctcgct tctctggctt tgattgcttt acggaccaac atctggctca
    14161 tttcgctatt ttcctgcaaa taaaagccca tttgttcact gatgacgtta tctactacgg
    14221 tacgagcaac tggcgtacca agtttttctt ttgtttgccc ttcaaattgc agaagatttt
    14281 caggtacacg gatagaaagt acagcagcta acccttctct gaaatcacta ccttccagat
    14341 tcttgtcccg ttctttcagc aaaccaactt ttctcgcata ttcattataa gatttcgtca
    14401 tcgccgcttt cataccagct tcatgggttc ccccatcttt tgtccgaacg ttattgacaa
    14461 aagataaaac attttctgaa taaccgtcat tgtattgata agcgacctct acttcgatac
    14521 cttctttttc tcctgaaaaa taaacaactg gtgtcaatgt atctttttct tcattcagat
    14581 aatcaacaaa ttctttgatt ccttcttcgt aatgaaaaat ttccttgact ggttcttctc
    14641 cacgaagatc agaaagagag attttaacgc cttttagaag aaaggcagat tctcttaacc
    14701 gttcggctaa aatttcatat gaaaatttcg ttgtactgaa tattgtatca tctggtaaaa
    14761 aatggacagt cgtcccgttt gctttttttg tttttcctat ctttttcaaa gtaccgtcag
    14821 gtttaccgcc gtttttaaat ttctgctgat actctacgcc atctctaacg atcgttacaa
    14881 ttagccattt cgataaggca tttaccacac tggctcctac accgtgcagt ccaccagaag
    14941 ttttgtaccc tccttgacca aactttccgc cagcatgcag cacagtaaaa atcacttcaa
    15001 ctgttggtat tccggaagca tgcattccta caggcatccc acgtccgcta tctgcaacag
    15061 ttatactatt gtcttcatgg atggtcacat caatctcact gccataacct gataatgctt
    15121 cgtcaactgc attatcgacg atttcataga ctaaatgatg caagcctcgg ctgtcagtgg
    15181 atccaatgta catccctggt ctttttctaa ctgcctctaa tccttccaat acttggatgg
    15241 acgagtcatt gtattcgttg tttacttttt ttgccaaggc aaacgctcct tcaataatac
    15301 tttgcggcaa aaccgcacca cgattatcat actagaaaag gtcgtgaaaa aaaagagggt
    15361 agacgagaaa tcaattttaa ttatctccta accgcttttt cgacattccc ctctatcgat
    15421 catctcttct ttgtggtcat ttttattttt ggttttcttt caacttacca agttcaatct
    15481 taatacaacg atccattacg atatctgtac gtcctgcttt tcttagcatc tcagctgctt
    15541 ctttgttttc aatccctaat tgtgcccaga acactttagc atcagttttc aaaaaatcac
    15601 ctgctatttc aggtaaaaat tcactccgtc taaatacatc tacgatatcg attggatcat
    15661 cgatttctgt caaattcgca tagactttct cgcctagaat ttcttcacca gccaataatg
    15721 gatttactgg tatgatttta tatccattgt cttgcaatac ctgtgcaacc atgtaacttg
    15781 ttcgttctgg tttattgctc aagcctacga cggcaatatt ttttgcttct tccaaatagt
    15841 tgaaaatcgt ttcttgtgtt ggatttttta cagacatttc tgccacccct tgcttttctt
    15901 tttattccat ctattatact tgtatctttc attttttcaa ttgttcctgt tacctattca
    15961 tgctaaaata aaggatagtt ttcaacttga aagagggtaa aaaatgaaat tgatcttatt
    16021 acttatttta gcatatcttt taggttctat tccttccggt gtctggattg gcaaattgtt
    16081 tttcaaaaaa gatatccgac aacacgggag cggtaatact ggaacgacca atacatttcg
    16141 tgtattaggc aaaacagccg ggattgttgt cctgcttatg gatattttaa aaggtacatt
    16201 ggctacttgt ttgccgatga tcttccacct ttctgggatt aaccctcttt ggtttggggt
    16261 atgcgccatt cttggccata catttccgat ttttgctggg ttcaaaggag gaaaagctgt
    16321 tgcgacaagt gcaggaatgc ttttaggatt taatcctgta ttttttgttt attcttgtat
    16381 catctttatc gttagtttat tttgtacaag tatggtgagt ttgagcagta tgatcagtgc
    16441 agtattgatc actttatcta cgatcgtcct tccttatgta gctcctgtga ttttggctaa
    16501 gcccaattgg ttactgacga tcattgcctt tttagtttct tcctttatct tttatcggca
    16561 taaagacaat atcaagcgta tcagaaacca tactgaaagt cgaatcccgt ttggtttgaa
    16621 tagcagcaag aaaaaatcct gatacttttt cattccaaag agataaaacg ggagtgtgac
    16681 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
    16741 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
    16801 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
    16861 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
    16921 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
    16981 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
    17041 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
    17101 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
    17161 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
    17221 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
    17281 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
    17341 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
    17401 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
    17461 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
    17521 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
    17581 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
    17641 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
    17701 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
    17761 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
    17821 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
    17881 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
    17941 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
    18001 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
    18061 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
    18121 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
    18181 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
    18241 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
    18301 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
    18361 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
    18421 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
    18481 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
    18541 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
    18601 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
    18661 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
    18721 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
    18781 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
    18841 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
    18901 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
    18961 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
    19021 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
    19081 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
    19141 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
    19201 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
    19261 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
    19321 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
    19381 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
    19441 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
    19501 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
    19561 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
    19621 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
    19681 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
    19741 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
    19801 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
    19861 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
    19921 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
    19981 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
    20041 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
    20101 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
    20161 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
    20221 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
    20281 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
    20341 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
    20401 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
    20461 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
    20521 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
    20581 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
    20641 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
    20701 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
    20761 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
    20821 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
    20881 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
    20941 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
    21001 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
    21061 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
    21121 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
    21181 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
    21241 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
    21301 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
    21361 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
    21421 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
    21481 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
    21541 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
    21601 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
    21661 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
    21721 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
    21781 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
    21841 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
    21901 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
    21961 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
    22021 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
    22081 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
    22141 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
    22201 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
    22261 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
    22321 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
    22381 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
    22441 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
    22501 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
    22561 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
    22621 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
    22681 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
    22741 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
    22801 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
    22861 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
    22921 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
    22981 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
    23041 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
    23101 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
    23161 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
    23221 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
    23281 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
    23341 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
    23401 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
    23461 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
    23521 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
    23581 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
    23641 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
    23701 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
    23761 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
    23821 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
    23881 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
    23941 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
    24001 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
    24061 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
    24121 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
    24181 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
    24241 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
    24301 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
    24361 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
    24421 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
    24481 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
    24541 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
    24601 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
    24661 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
    24721 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
    24781 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
    24841 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
    24901 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
    24961 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
    25021 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
    25081 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
    25141 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
    25201 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
    25261 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
    25321 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
    25381 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
    25441 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
    25501 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
    25561 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
    25621 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
    25681 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
    25741 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
    25801 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
    25861 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
    25921 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
    25981 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
    26041 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
    26101 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
    26161 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
    26221 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
    26281 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
    26341 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
    26401 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
    26461 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
    26521 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
    26581 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
    26641 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    26701 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    26761 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    26821 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    26881 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    26941 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    27001 cagtaattcc gttgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    27061 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    27121 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    27181 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    27241 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    27301 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    27361 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    27421 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    27481 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    27541 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    27601 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    27661 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    27721 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    27781 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    27841 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    27901 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    27961 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    28021 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    28081 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    28141 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    28201 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    28261 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    28321 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    28381 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    28441 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    28501 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    28561 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    28621 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    28681 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    28741 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    28801 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    28861 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    28921 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    28981 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    29041 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    29101 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    29161 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    29221 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    29281 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    29341 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    29401 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    29461 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    29521 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    29581 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    29641 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    29701 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    29761 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
    29821 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    29881 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    29941 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    30001 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    30061 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    30121 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    30181 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    30241 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    30301 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    30361 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    30421 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    30481 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    30541 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    30601 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    30661 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    30721 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    30781 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    30841 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    30901 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    30961 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    31021 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    31081 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    31141 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    31201 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    31261 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    31321 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    31381 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    31441 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    31501 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    31561 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    31621 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    31681 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    31741 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    31801 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    31861 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    31921 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    31981 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    32041 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    32101 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    32161 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    32221 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    32281 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    32341 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    32401 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    32461 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    32521 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    32581 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    32641 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    32701 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    32761 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    32821 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    32881 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    32941 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    33001 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    33061 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    33121 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    33181 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    33241 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    33301 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    33361 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    33421 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    33481 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    33541 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    33601 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    33661 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    33721 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    33781 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    33841 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    33901 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    33961 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    34021 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    34081 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    34141 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    34201 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    34261 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    34321 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    34381 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    34441 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    34501 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    34561 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    34621 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    34681 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    34741 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    34801 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    34861 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    34921 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    34981 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    35041 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    35101 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    35161 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    35221 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    35281 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    35341 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    35401 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    35461 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    35521 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    35581 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    35641 caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac tcaagtattc
    35701 ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt tttgcaattc
    35761 ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca atgagatttt
    35821 ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt ccatgtaaga
    35881 taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt tttcagcagg
    35941 ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat aaagttcttg
    36001 ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga aataacggtc
    36061 atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca aaatcgtcaa
    36121 tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag gttgctgaat
    36181 cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc ctatcagtgt
    36241 cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc cgatttgact
    36301 gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca tccgtttatc
    36361 tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt caccgataga
    36421 cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg tcgattttcc
    36481 cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcaata aatctaaatg
    36541 ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca gtactttttt
    36601 ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta cttggtacaa
    36661 gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg
    36721 tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct tttcttcttg
    36781 ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat aatttccttt
    36841 atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc tatctagaaa
    36901 ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat agttttccaa
    36961 ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata gtaaatcggg
    37021 ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg ataaagtact
    37081 gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg cgttggtatc
    37141 tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac gtttttgcac
    37201 atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg ctcgaacagt
    37261 ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt catcggaaaa
    37321 ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg
    37381 atcaccgtcg ctgctatcga taccggcaat aatatttaag agacttgttt tacccgtccc
    37441 gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa
    37501 caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt tcaacatgtt
    37561 ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa tttttttggt
    37621 atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg ttctgatatg
    37681 ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac tcgttttttt
    37741 tgactttgct cgaaacgcaa atcggaaaga aaagttatca gatggtttgt tcatcgtata
    37801 agattctaac aaattgatat tcctgcgata gctgttcgca gaaaacgtcg tgattttttc
    37861 agcaacttcg tattcatcaa tgatttttct ttcaagataa taacctctta gcattttttt
    37921 cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc aaattccccg agttcatttt
    37981 tattccttca agtttgcgtt gctggataaa aaattgaacc atcgtttcat cataaatatc
    38041 agtcaagtta tctaaacttt caataatcaa ctgagtattt ttttgatatg tctctctgat
    38101 ttcttggata tcttcatcac taataaatgt ctgacgatca gcatatcttc tttgccaaaa
    38161 cattttcgga tggagaacga ttcgcattaa cctacggaca acaatgaacc aaaagccgat
    38221 aaaagacaaa atctggcttg tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa
    38281 acgataacca ttagaagaaa gcttgcccga acgataactt tcatccaatc catcccgttc
    38341 gatcgacaaa atcagtgctt gtatttcttg tacttcttgc ttttcatcat cggttaaatc
    38401 ttccaagtac agatcacgta tccgttcttg ataattttct atcactgctt cagtggctaa
    38461 aaattctttt gtattacgat caatttcctt tatttctttt cgcaaaactt caacgacctc
    38521 ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat
    38581 aggcaagaca atgatcccta tgaccaaagt cactaagatg acacccgccg tcagaaacaa
    38641 aagcaaagac cgctggtaaa acatcatatt attgattgaa aatggcaaaa taaaaatcgt
    38701 agccaaactg actgttccct ttacaccgcc aaatgttaag atcaagatct cgttcaactg
    38761 ttttttgaac tttttactgc catctttgaa tacataaaaa agactgatag aaatgaatct
    38821 gatcacaaaa agcatgacag agattaatac gataatcgct aataaaagac cgtttggata
    38881 taaaccgtct ccccatactg gcgagaacac ttgcgtcaat tcgattccta aaaatataaa
    38941 tactaatgca ttcagtgtaa aagcaatagt tgtccatgta ctgtgggaca agctcgacaa
    39001 ttctgcatca aacacagaga ttttcctaaa accagaagct tggagaatac ctgctgccac
    39061 tgcagcaatg attccagata catcaaatgc ctctgccaat acataggcta gaaaaggaag
    39121 caaaagttcg atcagcagat accctgtgac atcttgtgct gatgcttttt cgatcaaatg
    39181 aattattttt tgtttgaacc agacaagcaa aaagccgatc aaagctcccc caatactaga
    39241 aactactaga ctcatccccg cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag
    39301 agcaaactgg aaggcagtaa caccagatgc atcattcaaa agaccttctc cttccaaaat
    39361 atgcatcgcc tttttaggga tattcactcg tccagaaaga gaactgactg ctaccgcatc
    39421 tgtcggacct aacgctgctc caaaagcaaa gcaggcagca agtgggacac ttggcaacag
    39481 gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg actcctccaa atgccaagaa
    39541 caaaattgtc ccgaaatttt tgagaatcga agaaatatct gctttttctc cttctctaaa
    39601 taacaatggt gcaataatca taactaagaa caattcaggt tcaaaatcga ttgattgtcc
    39661 ccactctgtc agacctaata ccactccaag gaaaatttgg atcagtggag ctggaatcat
    39721 tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt actgcaaata caataactaa
    39781 ctcagcaaat tccatcgtcc atccccttta ttttttattt tgtgtaagca tttttaaaaa
    39841 ttgttctttt ttctttaaaa ttttggtttc gactacttca acctgtgttt ggttcccctc
    39901 taattcacac tgtacacgtt ttgactccaa acgaagcaga tcatgtttca gacgttccaa
    39961 ctgacgatac atcaatatat ttcttttacg caacttacaa agtatttgac ggcggtcatc
    40021 catcgaaagg gcgccatagc gatcatgcaa cagttttttt tgttgttcaa aactctctct
    40081 cattttccag atctcctata cacaaacttc catcattata gcaaagttct ttttggatga
    40141 aaaatatttc attgtttcat cgtttattgc tagttagaaa tttccataaa tttttcaacg
    40201 cctcattcta ttccaaatag tatcagatat caagtaaaac ttaagaaaga taaaagtttc
    40261 tgacaaaagg atgtttactt tcccattcct gcctttcttg ttcttttatc acaacaaaaa
    40321 aatggggctg cgacaaaaat cttgtcacag tcccttattc tgaataaacg gtgtggaaga
    40381 gacagcctat cagtagtaat aagacacatc tctttcacag cctcattttt gcgtaaaaat
    40441 cgatttattc gaaaaattcc ccgtatacat cttctacacg ttgatctgga ttgatcacga
    40501 tatacaagtt acctgttttt tcgctgattt ttgttgattg atattgattg tctaatcctt
    40561 ctgttccttt atctagatat gggaagtaca caagatcagg tgtgccattt cggtaaagga
    40621 tttccattcg ctcgatatct tcgtatttca atgcgcgatt aaacatgccc atttccagtc
    40681 cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc atggaagatt tcgattttaa
    40741 atccagcaca cggatggatt tcaacaaatt cactgccatt gattcgtcca aaacttgttg
    40801 tgatttgttt gatccaaaga tcgccgatat gacggcggtt gatcgtccag gtttctccat
    40861 ttctaaaata aaattttagc gctgtcattt ctttactcat taaaaagact cctttatgat
    40921 gtaatttatt cttgtactac ctcagtttag cacaaattta tgtaagtggt cacatcttat
    40981 gttttttctt ctaatgacgt atctgcccac tttaagtagc caacaaagct gagcagatac
    41041 agtctagtgt tttttaaatc aatttttctt tcgataaaaa atccaaaacc tgttttttat
    41101 cattttttat tctttgttct acgacggctt tttcagctaa ttgcaaagct tcactaaccc
    41161 acttaccagg tttttttcca gtttcttcta gcaagatttt accatctact gctaattcgc
    41221 ttaatgactt gataggtaaa cgatcatagg attctcctac ggcatccatc tgaggtgttt
    41281 cgtcaaaata gatcatcaat tcttctactt gcagtgcatg ttttttgcct aagaagtaca
    41341 attcgaatgg ttccaaagct ccagtttctc gttgtcgtag tccttggaca agtgcttgaa
    41401 cgtctttaat catttggtta gaacacttcc acgatttcaa aaaggaacga atctcatttt
    41461 cttttagatc cagttggctg atcaataatg cccatgcttg gctctcttct ttcaatggga
    41521 cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc
    41581 aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca tttcccaaga
    41641 gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat agctcgtggt
    41701 tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga ctgacaaaac
    41761 gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga ttgcctacag
    41821 ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg atttctccat
    41881 cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct tctgccaacg
    41941 aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat tccgtccgaa
    42001 atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca tgttcgatcc
    42061 caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga
    42121 tatccacatc atggattggc tggttcagca agatatcacg aacacttcca cctacaaaat
    42181 aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt tggtattcca
    42241 gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg tcctgagata
    42301 aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca aggccaggac
    42361 cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt tgattgaatt
    42421 tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta gccatgatca
    42481 tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt tcagaagcct
    42541 ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct acttcttcag
    42601 ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc tggatatatt
    42661 catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt ttccctccaa
    42721 aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga aaatcgaatt
    42781 tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata cataattgaa
    42841 agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta tcattatagg
    42901 aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt tagcagaagg
    42961 gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc cagcttatac
    43021 gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg gtaaacgttc
    43081 cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga tctggcaaag
    43141 ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac ttgcgggttt
    43201 agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga
    43261 tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt ccctattggc
    43321 atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac gtatgatgta
    43381 tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag atggcggata
    43441 ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac cactactgat
    43501 gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct ctggtaattc
    43561 aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta agaggatcgt
    43621 ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag tggaagggca
    43681 aggctttact tatttgaagc cacagacaag attactgaaa aaactataag aaaaaagaca
    43741 aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt tagtactata
    43801 ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt gtctgcaaaa
    43861 ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct ccatcttcta
    43921 gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct ttttcttgaa
    43981 aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa gaagaaaatg
    44041 gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg aatatacatt
    44101 ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg tctgtgtccg
    44161 taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat agatcactga
    44221 tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg cttaaaatcg
    44281 cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta aaagatacat
    44341 gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc atttttctca
    44401 cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt caagtgttaa
    44461 ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta agaaaaaggc
    44521 atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt catagtgtac
    44581 agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc attctgcata
    44641 aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat atctttcatc
    44701 atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa
    44761 agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag cattttccga
    44821 ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt ctaacactgt
    44881 ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata gatataacga
    44941 ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct gctgattttc
    45001 tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc tttcttcttt
    45061 cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac ttaaggattg
    45121 tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg ccaacaatgt
    45181 ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt cagctagcgc
    45241 aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt ttgcttcact
    45301 cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc tttgcggata
    45361 gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga tctcattatc
    45421 ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt attggttgat
    45481 cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca attcttcgcc
    45541 tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc
    45601 ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg tattcatttc
    45661 aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc aataattctt
    45721 tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca agtatttttt
    45781 tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa atctgttcca
    45841 gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt agttggaaat
    45901 caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa aattttttca
    45961 tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca aacagtttac
    46021 cataatttga caggttttct gcctctaatt gataaaaata gatcatttct tcgatcatcc
    46081 cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac agattttgca
    46141 aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca atctgctttc
    46201 cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc agattgatgg
    46261 tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc
    46321 ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt gtatgttttt
    46381 gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata aacatggatt
    46441 gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt aaatgattgc
    46501 attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat aacgggttga
    46561 tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt ggctgatcaa
    46621 tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc gtcaacgcat
    46681 cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt attttaccat
    46741 tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg caaataaata
    46801 aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga atctctgtct
    46861 cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc ctagagccac
    46921 aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct ggtttgaatg
    46981 caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta cgacctttgc
    47041 gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact ttttcgccgt
    47101 tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt gcatcttttt
    47161 tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt gccatggata
    47221 gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac tttctgagtg
    47281 gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca cgatatcata
    47341 tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag tcatagaaac
    47401 gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt aaaatgccta
    47461 ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg cttttctgat
    47521 ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat tgacaaaaac
    47581 gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca ggcgttttcc
    47641 tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat tcaacacagc
    47701 cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag gcagacgttc
    47761 caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa gataccggaa
    47821 ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct ttacaagatc
    47881 ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac ccgctactcg
    47941 cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga tatcagaacg
    48001 gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt atacttttcc
    48061 gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt cagaaacgaa
    48121 gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc
    48181 tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa tcaaactaaa
    48241 cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca ctgcttcatc
    48301 taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat cgcctagccc
    48361 taaggaataa aattcataga tatcgtttct catttctgga ttatctactt tattgactgc
    48421 taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat ctgcgtctgt
    48481 cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa tcgcgatttc
    48541 tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc
    48601 gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat cacgagttac
    48661 tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga
    48721 cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat tatttcctcc
    48781 tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca agcatagatt
    48841 gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt caaaagaaaa
    48901 actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat tatttttcag
    48961 aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa atgtctccca
    49021 tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt ggttcttctt
    49081 ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt tcttctggat
    49141 ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca tgtggtgtcg
    49201 caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca gggaacactt
    49261 caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact gtgccaactg
    49321 gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa
    49381 cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca ttgacagcca
    49441 atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg actagaccgt
    49501 caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca cgtccactga
    49561 gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct ctttcgtttt
    49621 ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt tcgatgattt
    49681 taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga tcttccacca
    49741 ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct ttgacaacat
    49801 ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata tcttcccaaa
    49861 cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct ttatctttgc
    49921 cgatcgtact gataacgact aaatctaata catcaccgac ttttactact tcgctgatat
    49981 cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact ccagcccctt
    50041 cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc
    50101 caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg ttctcttgat
    50161 tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg taagtacggt
    50221 actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc gttttccttg
    50281 aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac cttttttcaa
    50341 aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg tatcgatacg
    50401 aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat aatggtctct
    50461 ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt cttggttctc
    50521 tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt tcacttctgc
    50581 attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac cgcgttcccc
    50641 tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag agacttcaga
    50701 aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg atgctatgaa
    50761 tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga tcagcgaaac
    50821 tagcccttct tcatctgaaa aagatacatt gttcgtaata gctaaataag ttactgcgcg
    50881 atacatggca ccggtatctg tataaatata attcaattct tttgctaata tcttggcaac
    50941 ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg tcatgatata
    51001 actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa ttcttaattc
    51061 ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa acaattgatc
    51121 agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt gaacagtcgt
    51181 tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc tgcttgaatc
    51241 ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg caatactgct
    51301 ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg tactagattc
    51361 tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt ctactactga
    51421 tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat ttctgatcca
    51481 gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa atagcgaaag
    51541 aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa cttcatcatc
    51601 tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg tagattgatg
    51661 tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt ataccatagg
    51721 aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa atttagtaaa
    51781 atcgttcccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg cagaagttct
    51841 gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt ttcgccaaaa
    51901 taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag catctgctgc
    51961 aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt
    52021 tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg agcttgctct
    52081 gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag
    52141 aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact ttcttcctga
    52201 tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg caaatcataa
    52261 tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt cgtagcaatg
    52321 agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg agctgcatca
    52381 agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata caatcgttcg
    52441 acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc catgaagtgt
    52501 tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg
    52561 actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat atctcttgct
    52621 actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg
    52681 atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga cacgcaatga
    52741 gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa aagagaagga
    52801 ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg acggaggacg
    52861 tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt
    52921 ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac gatgatgacc
    52981 agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc
    53041 aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc ttggccggga
    53101 cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct gtatcgcttc
    53161 ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt gtccatctac
    53221 attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca aacgttcgat
    53281 tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg cccactcaat
    53341 aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct ggtcaattgt
    53401 ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat tttgctgtaa
    53461 taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt gtgctaacaa
    53521 aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt tttttgccag
    53581 ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta aaaaatctgc
    53641 tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag tatcaatcaa
    53701 aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag tataaaaagg
    53761 acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa ttacttgaac
    53821 agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt caaaacgcaa
    53881 accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg ttagctttgc
    53941 ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa
    54001 cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt gggtaaatcc
    54061 ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag aactaatccg
    54121 cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat tctccttaag
    54181 ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg tattgcatgc
    54241 ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg aatcgtttta
    54301 tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga aacatatgta
    54361 aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa aatataaata
    54421 taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag ccgggatctg
    54481 tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta tctatagtta
    54541 attgactagt tttttcacgg accctatagg acccatttat attatttcaa ctttttcagc
    54601 cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat ttctgcctct
    54661 ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc attgattgat
    54721 acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc ctgattcaat
    54781 actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt caatgttcct
    54841 tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc tgaatgattc
    54901 ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat cacataatcg
    54961 gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac tgctagatca
    55021 agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg cgcccaaata
    55081 ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg agactttcca
    55141 tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc ttcttttacc
    55201 aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta ataaccggct
    55261 ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa tattttcact
    55321 ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga ttatacctta
    55381 aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg gaatatccat
    55441 ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga tacacagctt
    55501 ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca ttcgcctggt
    55561 gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg ttcatcgtac
    55621 gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta atatcgcgat
    55681 atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat gaatcaagga
    55741 agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat ttagctacta
    55801 aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt gtttgttctg
    55861 gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca tcttctgttt
    55921 tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag aaattgattg
    55981 ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca ttcactcctt
    56041 gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca ccttgttctt
    56101 cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt tctccagagt
    56161 aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa aactcgttcg
    56221 ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt aaaggaaatt
    56281 cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat ttttctaaac
    56341 aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc ttgaattcca
    56401 cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga cctttgtcat
    56461 cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat ggttttgttt
    56521 cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata ccgaataatt
    56581 catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta aatgttctaa
    56641 atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact tttttaccga
    56701 agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg atcaattcgc
    56761 tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc atgatattat
    56821 cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc ttcttctcaa
    56881 ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa atgatgaaag
    56941 aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat agccgtttat
    57001 cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt agaacattgc
    57061 tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt taccggtaaa
    57121 tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta cgatagcaga
    57181 tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga ttactaaatc
    57241 cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg cagcagctaa
    57301 gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg taataatgat
    57361 gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt
    57421 gattaatcca ttgttcataa ccacttggat accacgagca acagctacta cgattgctac
    57481 accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact ctttcatgcc
    57541 ataagtgatt gctactacga tggacattac taagaataac atagtaattt ctgggaagta
    57601 ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg gaaggtttgt
    57661 taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat
    57721 caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat gtttttgttt
    57781 tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca tatcttcttt
    57841 acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg cataacgata
    57901 cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc
    57961 ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag gatttaccgt
    58021 tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta ctactgtatc
    58081 aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg tttcttctgc
    58141 cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa taagcatttt
    58201 ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg cccctgtttt
    58261 gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg ggattgcagc
    58321 aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga ttggcgccat
    58381 gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc cagcgacgat
    58441 gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa gtacagcgat
    58501 tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt ttttcttttt
    58561 tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt tggctctaat
    58621 gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg taaaactttt
    58681 gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta aagctttctt
    58741 ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt ttaaaaggag
    58801 aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa aaaatactca
    58861 aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc aaaaaaaaag
    58921 accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg ttgttcgaac
    58981 tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa aaagacagca
    59041 ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac atagtttgct
    59101 aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta aagaggcgta
    59161 ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc tgtaatgaca
    59221 cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata ataaatcggg
    59281 aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt atccggagaa
    59341 aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac tccggccgct
    59401 ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat cttcaaaaaa
    59461 ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac
    59521 atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg
    59581 actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct attttccagg
    59641 gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc
    59701 agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca aaataggtcg
    59761 tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc ttatataatt
    59821 tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa cacttataaa
    59881 aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg ttcagttcac
    59941 ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca cgttttagtt
    60001 tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta cgtccttcgt
    60061 ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa atttggaatt
    60121 tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg
    60181 attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca tgttttggat
    60241 gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc gaagtatcgt
    60301 aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga tccgtaacaa
    60361 cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg tacaataaat
    60421 agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg tcatgtttcc
    60481 caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt cctgcaagga
    60541 tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact ttctgcaatc
    60601 tctccattac aattattctc ctttattcat tccatcaaag aatagatctg gtgacacgtc
    60661 ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg atttcaaacc
    60721 aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg gtccttctac
    60781 tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc ctgaaacctg
    60841 aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag cgatgattgc
    60901 taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat atcgcttaag
    60961 taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt caaaaatatg
    61021 caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta aacttgtgac
    61081 ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc cttcctcgcc
    61141 aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta tctcctactg
    61201 ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc tgcccttttt
    61261 tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa ggtgactcaa
    61321 aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg ataaatacca
    61381 ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt
    61441 tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg acttgtcctc
    61501 gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct tttgtgtaat
    61561 aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta tatttacggt
    61621 attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat tcttcatcgt
    61681 ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc aatgtagcag
    61741 ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta tggatataat
    61801 tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt tccatttgct
    61861 gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta atattgatct
    61921 ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa tgtaaggctc
    61981 tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt catctaacat
    62041 ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat ggatgatcag
    62101 ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg aaccttctgg
    62161 cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct ccttcaacaa
    62221 agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa ttttacggtt
    62281 ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc ttggaaaatg
    62341 ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct gagtcgtaga
    62401 gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat tttctagcag
    62461 atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa tcccagcctc
    62521 tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt tcccatgatt
    62581 attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa
    62641 gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct ctttatctcc
    62701 ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt tcaatccaat
    62761 caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac gatctcttat
    62821 ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg tcagagtcaa
    62881 tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc
    62941 agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga tcatccgagt
    63001 aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga tgactaaaaa
    63061 gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca agtactgttc
    63121 caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta
    63181 gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc tctatcatat
    63241 cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa aagccgtcta
    63301 gacactcaga cggactggtt tcttttttct ttttcatagc attcctgaca gattccatgg
    63361 aacgttagcc gatgatcttt tactaaaaaa tgataacgtt cttctactac ttgctccact
    63421 tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc agttcaagca tagtaaatga
    63481 tgatggaaat gtttggcgcc ttcttttctc aaatcataac gggctaatcc atcattaaaa
    63541 cttactttat cgacaatttt caattctgtc aagatttcaa gggttcgata aacagtagca
    63601 agtccaattt ccggactctt ttttttcact aaaaagaaga tttcttctgc agataaatgc
    63661 tctttctcat tttccaaaag cacaagcaga gtagcttctc tttgcggtgt cagcttaaac
    63721 cccgcttcat gtagctgctg ttttgttttt ttcatcgccg aaatagcatt catgaccatt
    63781 tactcaaccc caacaattta taataattac aaataaaaaa agatataaca ctatgttaat
    63841 taaaattatt ataagctagt taagaaaaac atgcaaggga ttctcaatta gattttttga
    63901 ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg
    63961 cgcgcttaaa tgctcctttg ctgataccaa aagctttcat gatttcttct ggatccgatt
    64021 tatctgtaaa gttgatttgc tgatctgcag aacgttctaa aatggttaaa atcatttgtg
    64081 catcatctga tatagcttca tatgctcttg gcttcaatga aagattcagt acaccgtctg
    64141 gtcggacacc aatcactcga ccttttatct gctcgcctag acgaggttca ttatatcgtt
    64201 cagaaggatg gataaaccca agatagaaat cttccgttaa aacgtacgta cctgttaatt
    64261 tcaagcggta tgctgttcct atcacatctt tgtttttcat ttcttctgtt ccatatttgc
    64321 ttaaagattt gaatattttt tcatctgcta aactgcccca aatacggtct tttgcatcaa
    64381 ctctaaggga aatcatcaag cgatcccctt ttttaggcca tagctctgtc attgtcggca
    64441 attcatcaag agatacgaca aagtctttat ctggaagacc gatatcgaca aacgcaccta
    64501 aatcttttcg tgtagcagta acgattccaa aagcgtaatg accgatacgg cttttaggaa
    64561 tttgtgtagt gatccgattt tcatgttttt gattttggta accgaatcct tcaactgctt
    64621 caccgatttt gtgttcacct tctgatttgc ttaagccaaa ggtttgtccg tttttttgga
    64681 caaagtagtt tttttcattt tcatcaataa taatacctgt aaatatagtt gctaataaat
    64741 cgttcatttt tttctccctc tatagtgaaa ggttttctta atacatttct ttgctatcat
    64801 aacataatgt tatctacttg aatagctgag ccttggtttg gaaaggtgaa aaaaggagta
    64861 tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg gcaggctcac
    64921 agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat ccagttttac
    64981 agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc atcaatgcag
    65041 attttgttga agtggcatat agattataat agccttcccc attttcgatt ctggcttttt
    65101 ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca atcactttgc
    65161 tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata tcacttgctc
    65221 tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag gatgccgctt
    65281 cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca gctgctgctt
    65341 gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct tttttctttt
    65401 cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc aattgttcat
    65461 tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt gccgtcttgt
    65521 attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata gctgtttttt
    65581 ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct tgttgaatca
    65641 attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct gctttcaact
    65701 tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac tcattcactt
    65761 tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac tctgaatatc
    65821 ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct tgatgaatca
    65881 tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa
    65941 gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct actttttgat
    66001 gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct aaaacgagat
    66061 aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt tctaagtttg
    66121 attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct tctgcttcaa
    66181 acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta ccctctttgc
    66241 ttacagattg attttcagca tcggattcac tatttatttc atcgggctct gttgactttt
    66301 tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc gttttttcct
    66361 tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt tttacgtctt
    66421 ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt tcagtttctt
    66481 gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc attttgagca
    66541 gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa gcggatagta
    66601 tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt cttttttgtt
    66661 ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca tgaacagttg
    66721 ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt ggttcttcgt
    66781 cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa tctttcaatt
    66841 ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct tcagttgatt
    66901 tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt tgctgatctt
    66961 ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt tttttcgatc
    67021 cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt ttacgctacc
    67081 atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat ttacttaaaa
    67141 ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag tctcaagctt
    67201 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
    67261 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
    67321 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
    67381 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
    67441 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
    67501 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
    67561 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
    67621 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
    67681 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
    67741 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
    67801 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
    67861 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
    67921 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
    67981 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
    68041 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
    68101 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
    68161 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
    68221 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
    68281 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
    68341 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
    68401 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
    68461 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
    68521 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
    68581 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
    68641 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
    68701 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
    68761 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
    68821 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
    68881 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
    68941 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
    69001 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
    69061 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
    69121 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
    69181 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
    69241 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
    69301 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
    69361 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
    69421 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
    69481 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
    69541 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
    69601 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
    69661 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
    69721 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
    69781 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
    69841 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
    69901 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
    69961 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
    70021 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
    70081 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
    70141 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
    70201 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
    70261 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
    70321 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
    70381 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
    70441 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
    70501 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
    70561 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
    70621 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
    70681 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
    70741 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
    70801 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
    70861 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
    70921 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
    70981 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
    71041 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
    71101 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
    71161 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
    71221 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
    71281 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
    71341 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
    71401 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
    71461 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
    71521 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
    71581 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
    71641 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
    71701 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
    71761 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
    71821 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
    71881 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
    71941 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
    72001 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
    72061 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
    72121 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
    72181 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
    72241 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
    72301 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
    72361 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
    72421 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
    72481 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
    72541 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
    72601 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
    72661 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
    72721 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
    72781 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
    72841 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
    72901 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
    72961 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
    73021 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
    73081 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
    73141 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
    73201 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
    73261 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
    73321 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
    73381 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
    73441 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
    73501 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
    73561 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
    73621 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
    73681 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
    73741 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgtact ccttctttca
    73801 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
    73861 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
    73921 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
    73981 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
    74041 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
    74101 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
    74161 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
    74221 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
    74281 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
    74341 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
    74401 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
    74461 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
    74521 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
    74581 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
    74641 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
    74701 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
    74761 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
    74821 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
    74881 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
    74941 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
    75001 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
    75061 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
    75121 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
    75181 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
    75241 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
    75301 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
    75361 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
    75421 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
    75481 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
    75541 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
    75601 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
    75661 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
    75721 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
    75781 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
    75841 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
    75901 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
    75961 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
    76021 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
    76081 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
    76141 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
    76201 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
    76261 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
    76321 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
    76381 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
    76441 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
    76501 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
    76561 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
    76621 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
    76681 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
    76741 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
    76801 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
    76861 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
    76921 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
    76981 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
    77041 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
    77101 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
    77161 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    77221 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    77281 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    77341 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    77401 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    77461 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    77521 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    77581 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    77641 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    77701 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    77761 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    77821 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    77881 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    77941 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    78001 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    78061 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    78121 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    78181 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    78241 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    78301 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    78361 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    78421 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    78481 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    78541 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    78601 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    78661 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    78721 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    78781 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    78841 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    78901 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    78961 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    79021 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    79081 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    79141 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    79201 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    79261 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    79321 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    79381 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    79441 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    79501 taa
//
LOCUS       DAYYCM010000005        78790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000005 DAYYCM010000000
VERSION     DAYYCM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.005"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACHAY1_000349"
     CDS             complement(<1..434)
                     /locus_tag="ACHAY1_000349"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8592380.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
     gene            680..1627
                     /locus_tag="ACHAY1_000350"
     CDS             680..1627
                     /locus_tag="ACHAY1_000350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8592381.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(1838..3910)
                     /locus_tag="ACHAY1_000351"
     CDS             complement(1838..3910)
                     /locus_tag="ACHAY1_000351"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8592382.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(3843..4220)
                     /locus_tag="ACHAY1_000352"
     CDS             complement(3843..4220)
                     /locus_tag="ACHAY1_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8592383.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(4349..4909)
                     /locus_tag="ACHAY1_000353"
     CDS             complement(4349..4909)
                     /locus_tag="ACHAY1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8592384.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            5059..6351
                     /locus_tag="ACHAY1_000354"
     CDS             5059..6351
                     /locus_tag="ACHAY1_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8592385.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(6411..7529)
                     /locus_tag="ACHAY1_000355"
     CDS             complement(6411..7529)
                     /locus_tag="ACHAY1_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8592386.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="ACHAY1_000356"
     CDS             complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="ACHAY1_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8592387.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="ACHAY1_000357"
     CDS             complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="ACHAY1_000357"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8592388.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10735..11334
                     /locus_tag="ACHAY1_000358"
     CDS             10735..11334
                     /locus_tag="ACHAY1_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8592389.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="ACHAY1_000359"
     CDS             complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="ACHAY1_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8592390.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(13029..13373)
                     /locus_tag="ACHAY1_000360"
     CDS             complement(13029..13373)
                     /locus_tag="ACHAY1_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8592391.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(13510..14655)
                     /locus_tag="ACHAY1_000361"
     CDS             complement(13510..14655)
                     /locus_tag="ACHAY1_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8592392.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(14835..15809)
                     /locus_tag="ACHAY1_000362"
     CDS             complement(14835..15809)
                     /locus_tag="ACHAY1_000362"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8592393.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(15889..16764)
                     /locus_tag="ACHAY1_000363"
     CDS             complement(15889..16764)
                     /locus_tag="ACHAY1_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8592394.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            16894..18801
                     /locus_tag="ACHAY1_000364"
     CDS             16894..18801
                     /locus_tag="ACHAY1_000364"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8592395.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(19342..22620)
                     /locus_tag="ACHAY1_000365"
     CDS             complement(19342..22620)
                     /locus_tag="ACHAY1_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8592396.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(22891..23436)
                     /locus_tag="ACHAY1_000366"
     CDS             complement(22891..23436)
                     /locus_tag="ACHAY1_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8592397.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(23448..24140)
                     /locus_tag="ACHAY1_000367"
     CDS             complement(23448..24140)
                     /locus_tag="ACHAY1_000367"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8592398.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(24159..24443)
                     /gene="macP"
                     /locus_tag="ACHAY1_000368"
     CDS             complement(24159..24443)
                     /gene="macP"
                     /locus_tag="ACHAY1_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8592399.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(24456..25019)
                     /locus_tag="ACHAY1_000369"
     CDS             complement(24456..25019)
                     /locus_tag="ACHAY1_000369"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8592400.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            25247..25909
                     /locus_tag="ACHAY1_000370"
     CDS             25247..25909
                     /locus_tag="ACHAY1_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8592401.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            26033..27229
                     /locus_tag="ACHAY1_000371"
     CDS             26033..27229
                     /locus_tag="ACHAY1_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8592402.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(27330..28706)
                     /locus_tag="ACHAY1_000372"
     CDS             complement(27330..28706)
                     /locus_tag="ACHAY1_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8592403.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(28743..29610)
                     /locus_tag="ACHAY1_000373"
                     /pseudo
     CDS             complement(28743..29610)
                     /locus_tag="ACHAY1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(29699..30724)
                     /locus_tag="ACHAY1_000374"
     CDS             complement(29699..30724)
                     /locus_tag="ACHAY1_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8592404.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(30742..31416)
                     /locus_tag="ACHAY1_000375"
     CDS             complement(30742..31416)
                     /locus_tag="ACHAY1_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8592405.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(31600..32493)
                     /locus_tag="ACHAY1_000376"
     CDS             complement(31600..32493)
                     /locus_tag="ACHAY1_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8592406.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(32567..34264)
                     /locus_tag="ACHAY1_000377"
     CDS             complement(32567..34264)
                     /locus_tag="ACHAY1_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8592407.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(34257..35570)
                     /locus_tag="ACHAY1_000378"
     CDS             complement(34257..35570)
                     /locus_tag="ACHAY1_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8592408.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(35570..39511)
                     /locus_tag="ACHAY1_000379"
     CDS             complement(35570..39511)
                     /locus_tag="ACHAY1_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8592409.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(39620..40078)
                     /locus_tag="ACHAY1_000380"
     CDS             complement(39620..40078)
                     /locus_tag="ACHAY1_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8592410.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(40376..40894)
                     /locus_tag="ACHAY1_000381"
     CDS             complement(40376..40894)
                     /locus_tag="ACHAY1_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8592411.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(40896..41513)
                     /locus_tag="ACHAY1_000382"
     CDS             complement(40896..41513)
                     /locus_tag="ACHAY1_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8592412.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(41510..42364)
                     /locus_tag="ACHAY1_000383"
     CDS             complement(41510..42364)
                     /locus_tag="ACHAY1_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592413.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(42361..43272)
                     /locus_tag="ACHAY1_000384"
     CDS             complement(42361..43272)
                     /locus_tag="ACHAY1_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592414.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(43313..44647)
                     /locus_tag="ACHAY1_000385"
     CDS             complement(43313..44647)
                     /locus_tag="ACHAY1_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8592415.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            44855..45685
                     /locus_tag="ACHAY1_000386"
     CDS             44855..45685
                     /locus_tag="ACHAY1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8592416.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(45929..46178)
                     /locus_tag="ACHAY1_000387"
                     /pseudo
     CDS             complement(45929..46178)
                     /locus_tag="ACHAY1_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(46369..46563)
                     /locus_tag="ACHAY1_000388"
     CDS             complement(46369..46563)
                     /locus_tag="ACHAY1_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592417.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(47068..47253)
                     /locus_tag="ACHAY1_000389"
     CDS             complement(47068..47253)
                     /locus_tag="ACHAY1_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592418.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(48103..48444)
                     /locus_tag="ACHAY1_000390"
     CDS             complement(48103..48444)
                     /locus_tag="ACHAY1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8592419.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(48444..48674)
                     /locus_tag="ACHAY1_000391"
     CDS             complement(48444..48674)
                     /locus_tag="ACHAY1_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8592420.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            48961..49299
                     /locus_tag="ACHAY1_000392"
     CDS             48961..49299
                     /locus_tag="ACHAY1_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592421.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(49504..50066)
                     /locus_tag="ACHAY1_000393"
                     /pseudo
     CDS             complement(49504..50066)
                     /locus_tag="ACHAY1_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(50798..51379)
                     /locus_tag="ACHAY1_000394"
     CDS             complement(50798..51379)
                     /locus_tag="ACHAY1_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8592422.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(51562..52188)
                     /locus_tag="ACHAY1_000395"
     CDS             complement(51562..52188)
                     /locus_tag="ACHAY1_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8592423.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(52210..53541)
                     /locus_tag="ACHAY1_000396"
     CDS             complement(52210..53541)
                     /locus_tag="ACHAY1_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8592424.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(53696..54097)
                     /locus_tag="ACHAY1_000397"
     CDS             complement(53696..54097)
                     /locus_tag="ACHAY1_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8592425.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(54183..54389)
                     /locus_tag="ACHAY1_000398"
     CDS             complement(54183..54389)
                     /locus_tag="ACHAY1_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592426.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(54484..55986)
                     /locus_tag="ACHAY1_000399"
     CDS             complement(54484..55986)
                     /locus_tag="ACHAY1_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8592427.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(56004..56459)
                     /gene="argR"
                     /locus_tag="ACHAY1_000400"
     CDS             complement(56004..56459)
                     /gene="argR"
                     /locus_tag="ACHAY1_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8592428.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(56557..57174)
                     /locus_tag="ACHAY1_000401"
     CDS             complement(56557..57174)
                     /locus_tag="ACHAY1_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8592429.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            57395..58768
                     /locus_tag="ACHAY1_000402"
     CDS             57395..58768
                     /locus_tag="ACHAY1_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8592430.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            58843..59955
                     /locus_tag="ACHAY1_000403"
     CDS             58843..59955
                     /locus_tag="ACHAY1_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8592431.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(60027..61664)
                     /locus_tag="ACHAY1_000404"
     CDS             complement(60027..61664)
                     /locus_tag="ACHAY1_000404"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8592432.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(61832..62440)
                     /locus_tag="ACHAY1_000405"
     CDS             complement(61832..62440)
                     /locus_tag="ACHAY1_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8592433.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(62824..63975)
                     /gene="queG"
                     /locus_tag="ACHAY1_000406"
     CDS             complement(62824..63975)
                     /gene="queG"
                     /locus_tag="ACHAY1_000406"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8592434.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(63994..64746)
                     /locus_tag="ACHAY1_000407"
     CDS             complement(63994..64746)
                     /locus_tag="ACHAY1_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592435.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(64887..65836)
                     /locus_tag="ACHAY1_000408"
                     /pseudo
     CDS             complement(64887..65836)
                     /locus_tag="ACHAY1_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(65837..66676)
                     /locus_tag="ACHAY1_000409"
     CDS             complement(65837..66676)
                     /locus_tag="ACHAY1_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8592436.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(66673..67419)
                     /locus_tag="ACHAY1_000410"
     CDS             complement(66673..67419)
                     /locus_tag="ACHAY1_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8592437.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            67657..69027
                     /locus_tag="ACHAY1_000411"
     CDS             67657..69027
                     /locus_tag="ACHAY1_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8592438.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(69082..69978)
                     /locus_tag="ACHAY1_000412"
     CDS             complement(69082..69978)
                     /locus_tag="ACHAY1_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8592439.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(70092..70262)
                     /locus_tag="ACHAY1_000413"
     CDS             complement(70092..70262)
                     /locus_tag="ACHAY1_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592440.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(70259..70936)
                     /locus_tag="ACHAY1_000414"
     CDS             complement(70259..70936)
                     /locus_tag="ACHAY1_000414"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8592441.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(71027..71932)
                     /locus_tag="ACHAY1_000415"
     CDS             complement(71027..71932)
                     /locus_tag="ACHAY1_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8592442.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            72107..73531
                     /locus_tag="ACHAY1_000416"
     CDS             72107..73531
                     /locus_tag="ACHAY1_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8592443.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            73577..74608
                     /locus_tag="ACHAY1_000417"
     CDS             73577..74608
                     /locus_tag="ACHAY1_000417"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8592444.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            74615..75802
                     /locus_tag="ACHAY1_000418"
     CDS             74615..75802
                     /locus_tag="ACHAY1_000418"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8592445.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            75958..77340
                     /locus_tag="ACHAY1_000419"
     CDS             75958..77340
                     /locus_tag="ACHAY1_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8592446.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            77377..77697
                     /locus_tag="ACHAY1_000420"
     CDS             77377..77697
                     /locus_tag="ACHAY1_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592447.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(77897..78565)
                     /locus_tag="ACHAY1_000421"
     CDS             complement(77897..78565)
                     /locus_tag="ACHAY1_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8592448.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        22555 a        16518 c        14074 g        25643 t
ORIGIN      
        1 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
       61 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
      121 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
      181 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
      241 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
      301 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
      361 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
      421 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
      481 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
      541 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
      601 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
      661 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
      721 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
      781 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
      841 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
      901 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
      961 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     1021 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     1081 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     1141 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     1201 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     1261 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     1321 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     1381 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     1441 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     1501 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     1561 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     1621 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     1681 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     1741 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     1801 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     1861 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     1921 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     1981 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     2041 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     2101 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     2161 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     2221 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     2281 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     2341 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     2401 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     2461 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     2521 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     2581 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     2641 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     2701 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     2761 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     2821 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     2881 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     2941 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     3001 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     3061 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     3121 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     3181 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     3241 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     3301 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     3361 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     3421 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     3481 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     3541 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     3601 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     3661 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     3721 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     3781 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     3841 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     3901 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     3961 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     4021 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     4081 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     4141 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     4201 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     4261 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     4321 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     4381 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     4441 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     4501 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     4561 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     4621 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     4681 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     4741 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     4801 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     4861 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     4921 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     4981 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     5041 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     5101 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     5161 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     5221 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     5281 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     5341 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     5401 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     5461 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     5521 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     5581 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     5641 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     5701 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     5761 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     5821 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     5881 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     5941 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     6001 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     6061 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     6121 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     6181 tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     6241 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     6301 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     6361 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     6421 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     6481 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     6541 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     6601 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     6661 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     6721 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     6781 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     6841 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     6901 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     6961 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     7021 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     7081 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     7141 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     7201 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     7261 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     7321 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     7381 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     7441 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     7501 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     7561 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     7621 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     7681 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     7741 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     7801 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     7861 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     7921 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     7981 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     8041 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     8101 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     8161 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     8221 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     8281 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     8341 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     8401 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     8461 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     8521 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     8581 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     8641 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     8701 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
     8761 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
     8821 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
     8881 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
     8941 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
     9001 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
     9061 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
     9121 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
     9181 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
     9241 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
     9301 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
     9361 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
     9421 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
     9481 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
     9541 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
     9601 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
     9661 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
     9721 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
     9781 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
     9841 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
     9901 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
     9961 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    10021 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    10081 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    10141 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    10201 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    10261 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    10321 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    10381 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    10441 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    10501 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    10561 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    10621 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    10681 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    10741 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    10801 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    10861 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    10921 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    10981 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    11041 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    11101 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    11161 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    11221 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    11281 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    11341 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    11401 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    11461 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    11521 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    11581 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    11641 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    11701 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    11761 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    11821 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    11881 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    11941 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    12001 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    12061 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    12121 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    12181 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    12241 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    12301 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    12361 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    12421 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    12481 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    12541 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    12601 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    12661 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    12721 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    12781 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    12841 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    12901 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    12961 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    13021 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    13081 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    13141 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    13201 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    13261 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    13321 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    13381 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    13441 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    13501 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    13561 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    13621 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    13681 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    13741 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    13801 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    13861 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    13921 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    13981 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    14041 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    14101 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    14161 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    14221 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    14281 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    14341 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    14401 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    14461 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    14521 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    14581 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    14641 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    14701 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    14761 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    14821 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    14881 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    14941 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    15001 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    15061 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    15121 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    15181 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    15241 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    15301 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    15361 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    15421 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    15481 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    15541 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    15601 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    15661 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    15721 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    15781 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    15841 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    15901 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    15961 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    16021 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    16081 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    16141 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    16201 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    16261 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    16321 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    16381 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    16441 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    16501 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    16561 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    16621 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    16681 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    16741 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    16801 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    16861 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    16921 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    16981 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    17041 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    17101 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    17161 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    17221 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    17281 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    17341 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    17401 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    17461 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    17521 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    17581 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    17641 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    17701 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    17761 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    17821 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    17881 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    17941 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    18001 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    18061 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    18121 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    18181 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    18241 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    18301 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    18361 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    18421 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    18481 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    18541 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    18601 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    18661 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    18721 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    18781 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    18841 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    18901 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    18961 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    19021 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    19081 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    19141 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    19201 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    19261 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    19321 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    19381 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    19441 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    19501 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    19561 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    19621 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    19681 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    19741 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    19801 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    19861 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    19921 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    19981 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    20041 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    20101 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    20161 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    20221 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    20281 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    20341 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    20401 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    20461 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    20521 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    20581 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    20641 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    20701 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    20761 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    20821 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    20881 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    20941 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    21001 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    21061 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    21121 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    21181 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    21241 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    21301 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    21361 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    21421 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    21481 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    21541 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    21601 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    21661 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    21721 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    21781 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    21841 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    21901 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    21961 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    22021 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    22081 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    22141 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    22201 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    22261 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    22321 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    22381 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    22441 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    22501 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    22561 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    22621 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    22681 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    22741 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    22801 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    22861 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    22921 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
    22981 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    23041 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    23101 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    23161 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    23221 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    23281 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    23341 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    23401 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    23461 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    23521 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    23581 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    23641 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    23701 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    23761 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    23821 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    23881 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    23941 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    24001 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    24061 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    24121 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    24181 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    24241 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    24301 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    24361 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    24421 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    24481 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    24541 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    24601 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    24661 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    24721 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    24781 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    24841 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    24901 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    24961 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    25021 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    25081 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    25141 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    25201 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    25261 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    25321 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    25381 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    25441 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    25501 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    25561 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    25621 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    25681 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    25741 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    25801 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    25861 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    25921 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    25981 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    26041 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    26101 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    26161 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    26221 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    26281 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    26341 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    26401 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    26461 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    26521 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    26581 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    26641 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    26701 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    26761 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    26821 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    26881 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    26941 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    27001 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    27061 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    27121 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    27181 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    27241 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    27301 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    27361 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    27421 acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc
    27481 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    27541 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    27601 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    27661 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    27721 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    27781 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    27841 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    27901 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    27961 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    28021 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    28081 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    28141 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    28201 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    28261 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    28321 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    28381 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    28441 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    28501 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    28561 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    28621 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    28681 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    28741 gattaaactg ttttcatata ccctaaaagg gataccacct aaaatgacta aaaggcaacc
    28801 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
    28861 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
    28921 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
    28981 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
    29041 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
    29101 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
    29161 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
    29221 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
    29281 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
    29341 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
    29401 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
    29461 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
    29521 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
    29581 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
    29641 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
    29701 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
    29761 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
    29821 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
    29881 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
    29941 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
    30001 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
    30061 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
    30121 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
    30181 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
    30241 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
    30301 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
    30361 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
    30421 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
    30481 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
    30541 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
    30601 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
    30661 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
    30721 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
    30781 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
    30841 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
    30901 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
    30961 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
    31021 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
    31081 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
    31141 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
    31201 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
    31261 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
    31321 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
    31381 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
    31441 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
    31501 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
    31561 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
    31621 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
    31681 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
    31741 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
    31801 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
    31861 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
    31921 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
    31981 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
    32041 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
    32101 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
    32161 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
    32221 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
    32281 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
    32341 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
    32401 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
    32461 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
    32521 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
    32581 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
    32641 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
    32701 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
    32761 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
    32821 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
    32881 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
    32941 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
    33001 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
    33061 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
    33121 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
    33181 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
    33241 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
    33301 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
    33361 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
    33421 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
    33481 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
    33541 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
    33601 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
    33661 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
    33721 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
    33781 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
    33841 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
    33901 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
    33961 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
    34021 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
    34081 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
    34141 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
    34201 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
    34261 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
    34321 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
    34381 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
    34441 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
    34501 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
    34561 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
    34621 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
    34681 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
    34741 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
    34801 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
    34861 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    34921 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    34981 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    35041 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    35101 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    35161 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    35221 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    35281 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    35341 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    35401 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    35461 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    35521 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    35581 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    35641 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    35701 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    35761 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    35821 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    35881 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    35941 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    36001 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    36061 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    36121 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    36181 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    36241 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    36301 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    36361 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    36421 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    36481 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    36541 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    36601 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    36661 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    36721 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    36781 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    36841 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    36901 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    36961 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    37021 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    37081 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    37141 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    37201 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    37261 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    37321 gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa
    37381 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    37441 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    37501 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    37561 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    37621 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    37681 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    37741 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    37801 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    37861 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    37921 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    37981 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    38041 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    38101 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    38161 tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    38221 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    38281 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    38341 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    38401 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    38461 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    38521 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    38581 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    38641 taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac
    38701 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    38761 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    38821 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    38881 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    38941 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    39001 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    39061 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    39121 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    39181 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    39241 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    39301 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt
    39361 cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc
    39421 caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc
    39481 tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag
    39541 cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa
    39601 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    39661 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    39721 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    39781 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    39841 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    39901 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    39961 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    40021 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    40081 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    40141 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    40201 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    40261 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    40321 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    40381 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    40441 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    40501 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    40561 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    40621 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    40681 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    40741 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    40801 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    40861 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    40921 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    40981 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    41041 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    41101 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    41161 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    41221 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    41281 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    41341 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    41401 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    41461 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    41521 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    41581 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    41641 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    41701 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    41761 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    41821 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    41881 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    41941 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    42001 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    42061 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    42121 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    42181 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    42241 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    42301 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    42361 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    42421 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    42481 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    42541 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    42601 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    42661 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    42721 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    42781 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    42841 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    42901 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    42961 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    43021 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    43081 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    43141 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    43201 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    43261 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    43321 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    43381 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    43441 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    43501 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    43561 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    43621 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    43681 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    43741 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    43801 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    43861 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    43921 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    43981 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    44041 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    44101 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    44161 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    44221 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    44281 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    44341 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    44401 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    44461 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    44521 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    44581 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    44641 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    44701 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    44761 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    44821 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    44881 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    44941 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    45001 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    45061 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    45121 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    45181 gcacgcgatc gtagatggct taggacctcg tcctgctgac agcgtgggca atccatggag
    45241 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    45301 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    45361 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    45421 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    45481 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    45541 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    45601 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    45661 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    45721 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    45781 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    45841 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    45901 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    45961 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    46021 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    46081 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    46141 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    46201 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    46261 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    46321 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    46381 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    46441 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    46501 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    46561 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    46621 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    46681 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    46741 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    46801 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    46861 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    46921 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    46981 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    47041 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    47101 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    47161 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    47221 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
    47281 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaacc
    47341 ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga
    47401 tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa
    47461 ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca
    47521 ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta
    47581 aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt
    47641 gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt
    47701 cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga
    47761 gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact
    47821 aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata
    47881 cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga
    47941 aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta
    48001 atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt
    48061 atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat
    48121 aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg
    48181 ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc
    48241 ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac
    48301 cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt
    48361 ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg
    48421 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
    48481 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
    48541 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
    48601 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
    48661 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
    48721 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
    48781 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
    48841 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
    48901 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
    48961 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
    49021 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
    49081 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
    49141 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
    49201 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
    49261 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
    49321 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
    49381 actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt
    49441 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
    49501 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
    49561 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
    49621 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
    49681 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
    49741 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
    49801 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
    49861 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
    49921 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
    49981 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
    50041 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
    50101 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
    50161 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
    50221 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
    50281 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
    50341 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac
    50401 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50461 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
    50521 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
    50581 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    50641 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
    50701 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    50761 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
    50821 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
    50881 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
    50941 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
    51001 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
    51061 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
    51121 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
    51181 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
    51241 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
    51301 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
    51361 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
    51421 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
    51481 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
    51541 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
    51601 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
    51661 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
    51721 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
    51781 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
    51841 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
    51901 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
    51961 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
    52021 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
    52081 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
    52141 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
    52201 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
    52261 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
    52321 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
    52381 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
    52441 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
    52501 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
    52561 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
    52621 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
    52681 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
    52741 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
    52801 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
    52861 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
    52921 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
    52981 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
    53041 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
    53101 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
    53161 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
    53221 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
    53281 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
    53341 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
    53401 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
    53461 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
    53521 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
    53581 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
    53641 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
    53701 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
    53761 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
    53821 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
    53881 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
    53941 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
    54001 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
    54061 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
    54121 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
    54181 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
    54241 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
    54301 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
    54361 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
    54421 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
    54481 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
    54541 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
    54601 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
    54661 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
    54721 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
    54781 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
    54841 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
    54901 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
    54961 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
    55021 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
    55081 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
    55141 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
    55201 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
    55261 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
    55321 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
    55381 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
    55441 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
    55501 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
    55561 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
    55621 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
    55681 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
    55741 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
    55801 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
    55861 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
    55921 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
    55981 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
    56041 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
    56101 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
    56161 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
    56221 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
    56281 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
    56341 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
    56401 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
    56461 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
    56521 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
    56581 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
    56641 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
    56701 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
    56761 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
    56821 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
    56881 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
    56941 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
    57001 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
    57061 attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg
    57121 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
    57181 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
    57241 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
    57301 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
    57361 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
    57421 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
    57481 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
    57541 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
    57601 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
    57661 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
    57721 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
    57781 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
    57841 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
    57901 gtttcttgtg cccgttttgt ttctttcttt gattatcggc tggtttgcta ttcccgagat
    57961 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    58021 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    58081 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
    58141 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    58201 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    58261 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    58321 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    58381 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    58441 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    58501 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    58561 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    58621 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    58681 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    58741 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    58801 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    58861 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    58921 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    58981 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    59041 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    59101 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    59161 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    59221 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    59281 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    59341 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    59401 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    59461 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    59521 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    59581 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    59641 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    59701 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    59761 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    59821 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    59881 ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg
    59941 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    60001 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    60061 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    60121 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    60181 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    60241 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    60301 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    60361 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    60421 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    60481 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    60541 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    60601 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    60661 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    60721 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    60781 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    60841 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    60901 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    60961 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    61021 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    61081 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    61141 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    61201 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    61261 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    61321 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    61381 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    61441 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    61501 atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga
    61561 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    61621 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    61681 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    61741 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    61801 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    61861 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    61921 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    61981 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    62041 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    62101 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    62161 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    62221 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    62281 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    62341 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    62401 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    62461 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    62521 aaacatgaca tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat
    62581 atttttttga tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat
    62641 gtttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    62701 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    62761 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    62821 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    62881 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    62941 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    63001 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    63061 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    63121 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac
    63181 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    63241 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    63301 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    63361 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    63421 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    63481 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    63541 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    63601 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    63661 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    63721 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    63781 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    63841 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    63901 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    63961 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    64021 tttttctttt ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat
    64081 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    64141 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    64201 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    64261 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    64321 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    64381 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    64441 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    64501 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    64561 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    64621 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    64681 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    64741 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    64801 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    64861 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    64921 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    64981 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    65041 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    65101 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    65161 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    65221 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    65281 aattctttta gtttttccac atatgcttct gcattttttt atagagtgtc gcattttctg
    65341 catctttttc agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt
    65401 ctagccaagc atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt
    65461 ctacattttt gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca
    65521 agttatcgaa ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg
    65581 catcgcttgc ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga
    65641 ctggtacgat actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca
    65701 agatcgaatt ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag
    65761 aagcctgctt tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg
    65821 atttttttgt tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa
    65881 ataaaaaagc aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc
    65941 aattgtaact ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag
    66001 ctgtaatgat cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga
    66061 tcaacatggc aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg
    66121 tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca
    66181 ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga
    66241 cgattccact aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc
    66301 caaataaaat atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta
    66361 atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct
    66421 gagtcacgaa accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt
    66481 aactaaaacc aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt
    66541 ctcccatcaa agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc
    66601 ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa
    66661 taaaggattg gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata
    66721 ggccaattga aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg
    66781 tttatttaaa agaataacct catcgaaata ttcttctact ttatgaagat catgatggac
    66841 gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc
    66901 acttaacgca tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc
    66961 tagagctctt gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct
    67021 tttggcatat tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt
    67081 tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa
    67141 tacatcaatt ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt
    67201 acggactgag tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa
    67261 ctctagcaaa gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt
    67321 aattttttgt ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc
    67381 ggctgaaagg tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt
    67441 ttacctaact ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat
    67501 gatgaaaaag tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca
    67561 tcaagagttc aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt
    67621 ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga
    67681 agtcttagtg cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg
    67741 tttatgggtg cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg
    67801 attgcaggat tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat
    67861 ccttcgactg gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac
    67921 ttgaccgctt ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct
    67981 gtaggatctt atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt
    68041 gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa
    68101 ttttggtttt cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg
    68161 gtaatcttat ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa
    68221 aatggtggat tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt
    68281 gcttcctatc aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa
    68341 aagacgatca ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt
    68401 gctattttta tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct
    68461 tttgtacaaa ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc
    68521 gtgattacag cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc
    68581 tatacacttg ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt
    68641 gcgccttttt atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat
    68701 tatcttctac caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca
    68761 agtattttgc ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa
    68821 aagaagcacg cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg
    68881 aattatgtga cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa
    68941 acgagaatct cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt
    69001 gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt
    69061 cgttcttttt tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata
    69121 attctccatg ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc
    69181 ggctatttga tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc
    69241 gctgttggta ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc
    69301 agaagcttct accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt
    69361 tgactctgct tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg
    69421 attcgtataa ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt
    69481 ttctgctgcc tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag
    69541 ctcacgtgct ttttggttag caactgctga taaaccagct attccctctt gatttccctg
    69601 aatttctgtc tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata
    69661 atcatttacg attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt
    69721 ttgtgtctct tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt
    69781 cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag
    69841 acccgcaact cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat
    69901 ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca
    69961 ctcattttca tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac
    70021 gcttcatatt cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt
    70081 ataagaaaat ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc
    70141 tgcattgttc gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg
    70201 tcttgatatt ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc
    70261 ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg
    70321 cctcagtcag tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct
    70381 gattttcctg tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt
    70441 ccaatgagag atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat
    70501 acataaattg tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc
    70561 gctgatacgg cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt
    70621 ctttttccgc cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg
    70681 ctgctcgttc caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt
    70741 aaaacggaaa ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta
    70801 tatctatttt tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag
    70861 cagaagcttc tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga
    70921 tgataagtgt acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac
    70981 cgccacaagt ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag
    71041 aaactgcggt ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat
    71101 tttcgttttt catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca
    71161 ttcggctaaa tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt
    71221 catccgtcag agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct
    71281 tgatactggt acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta
    71341 aatgctgggc gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga
    71401 ctgtttcaac agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat
    71461 cttttaaaag gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca
    71521 aggtttcttc cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag
    71581 aagagaccat cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag
    71641 cactcccacc aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa
    71701 acgtcgagtc tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat
    71761 ttttcacatg ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa
    71821 tagcaggacc aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca
    71881 attctttttc tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc
    71941 ctccttactc ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta
    72001 aaaaggaagc gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa
    72061 ccggtttact tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat
    72121 gatccaacgt gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt
    72181 aagcacttat tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac
    72241 gaatgcaaaa atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat
    72301 tatttttgat cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt
    72361 caaaccatgg ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac
    72421 caatttcttt ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt
    72481 tttcatctta ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc
    72541 agctttgagt acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc
    72601 ttctctaggt gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta
    72661 tcttgttact cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc
    72721 tgctgttatt tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac
    72781 tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca
    72841 aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc
    72901 aacaactgcc gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg
    72961 gatcgttggt ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct
    73021 tacacgcgtc atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc
    73081 ttactttttc tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt
    73141 gttctacttc cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt
    73201 tgaatatggt caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc
    73261 tttactagat aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc
    73321 ttctgggatg actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt
    73381 caaagcttat gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc
    73441 ttggaagatt acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa
    73501 attggctcca gctgatacaa tggaagatta acttcacgat gcagtagttc cttcccatta
    73561 agaaaggatc ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat
    73621 aaccttggag aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt
    73681 cagtttaaaa tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat
    73741 cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg
    73801 ccgcttttct ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa
    73861 tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg
    73921 cgaaaatgaa gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc
    73981 aggtacaatg gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac
    74041 ctctcgcggc accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa
    74101 cttgacggga gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc
    74161 ttatgctcct ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag
    74221 tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca
    74281 aaaaaatctt gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa
    74341 agaagccgct cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc
    74401 ttctagtgtg gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg
    74461 aaaactggca catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg
    74521 cttctcagca tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtaacaga
    74581 attcaaaatc aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac
    74641 aaaatttgcc gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat
    74701 caatttgatt ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac
    74761 ttatggaacg caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac
    74821 aaactttgac gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa
    74881 agcaaactgg gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc
    74941 aatcgatcat gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc
    75001 cagtagcgct tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa
    75061 aacagatcgc ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt
    75121 caattcaggt atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt
    75181 attagataca aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat
    75241 cgtcatcatg aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg
    75301 tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg
    75361 agacttagat gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat
    75421 acgcagagct cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt
    75481 agaagctggg aacttagaag catttggtca actgttaaat gattctcacc actctcttcg
    75541 ctatgattat gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca
    75601 agaaggtgtc ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct
    75661 tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt
    75721 tgtcggctat gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga
    75781 tccagaagct gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata
    75841 aaaataacct ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt
    75901 tttttattga cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg
    75961 tgggcgaatc aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt
    76021 attgactgca aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt
    76081 acaactaaat tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga
    76141 ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt
    76201 gccagagatc ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc
    76261 tattatatgg aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg
    76321 aaccatcatg tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc
    76381 tttggtaaaa caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag
    76441 accggaatct acacgactat aggaattggc gataacccgc tacttgctaa actagcctta
    76501 gacaatggct caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca
    76561 gaaaagttat ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca
    76621 aaacgtctga aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat
    76681 cacttgaaaa gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc
    76741 gatcgaagtt ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa
    76801 atcctagata gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc
    76861 gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg
    76921 gtaggatact ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg
    76981 aaaatcgaag cgacaaatgc tagcagagat cttgcagatg ctttattgac cattttccac
    77041 cagcattacc actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac
    77101 gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa
    77161 gttgattttg ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg
    77221 catagtctct tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca
    77281 ggtggtatga gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga
    77341 ctataacgag tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga
    77401 agaactagtc aacggtatca aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa
    77461 caacaaatga cattgcaaga aatcgatagt atactgacag aagccttttt atctcgtaaa
    77521 gcagtaatca ttcagctcaa tttaaaagat gaatttggaa gactcttgga tgaggtcgaa
    77581 gggcacttca ttggtgaagc ctatgaagat tactttatga ttgaccatca gccgatattg
    77641 tgggaagacg tacggtatat tgagttaaag gaaacaaaaa aatggtttgt gacatgagag
    77701 gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga tttttcgaaa attgcttctt
    77761 aaatttttgt ggaattttgg cttatttttc gaagaagcaa cttctggaac aacgtatatt
    77821 agatatcaga ggttgtgaaa aagtcgctta gttttgagta acaagccaag gcgctgattt
    77881 cacaacctct gtctatctat ccatctattc ggtaagattc atgcatgacg tcttctttat
    77941 ttagtatgta gagccctttt tcttctgacc attcaacata aatcagctgt tttacatctg
    78001 catagccttc tttcttcaag ttctccataa gccatgcttc agtcaattga ttggactgaa
    78061 gcactttatg ctgaatttgc ccttcatcta caagcaggat cgataaagct ttgtcttcat
    78121 ctgatttttt caatacactc atttgaccgg cattctctag gataacatgt ttggcatttt
    78181 caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt
    78241 tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat tagaacagat ggctcgcctt
    78301 tgacccaacg attgaccttt tcattttttt ggacgatacg ttcgatacca taaatcatta
    78361 ctgcccaaag cgcaatggca aataggacat gccctacatg gacattgtca tcataggttg
    78421 attcttctaa gatcccacct aaaacaagcg tatagactaa atcaaacggt gtgatttctg
    78481 acatggattt cttcccaagc aaacgtacca ctaataataa accgatcaac ccgaacacca
    78541 gtttcattgt aattgaaaaa atcatttttt ctccccctaa ctatctgtta attaaattat
    78601 atatgctttc tcacgaacac gcatggaata tacaaggaag aaatgtatcc ttttactaaa
    78661 aaaaggactg tgacactgta gtgaccccca aaagttagac ttttttggag tggagaaatc
    78721 cactcctttt ttttatgctc taaaatgata tttttacaca accgttacgg ttgttttacg
    78781 aaattggacg
//
LOCUS       DAYYCM010000006        68572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000006 DAYYCM010000000
VERSION     DAYYCM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.006"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..629
                     /locus_tag="ACHAY1_000422"
     CDS             111..629
                     /locus_tag="ACHAY1_000422"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8592449.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            757..1440
                     /locus_tag="ACHAY1_000423"
     CDS             757..1440
                     /locus_tag="ACHAY1_000423"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8592450.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1749..1822
                     /locus_tag="ACHAY1_000424"
     tRNA            1749..1822
                     /locus_tag="ACHAY1_000424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1783..1785,aa:Arg,seq:tct)
     gene            1981..2340
                     /locus_tag="ACHAY1_000425"
     CDS             1981..2340
                     /locus_tag="ACHAY1_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8592451.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2463..4094
                     /locus_tag="ACHAY1_000426"
     CDS             2463..4094
                     /locus_tag="ACHAY1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8592452.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4156..4797)
                     /locus_tag="ACHAY1_000427"
     CDS             complement(4156..4797)
                     /locus_tag="ACHAY1_000427"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8592453.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4983..5059
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5079..5387
                     /gene="rplU"
                     /locus_tag="ACHAY1_000428"
     CDS             5079..5387
                     /gene="rplU"
                     /locus_tag="ACHAY1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8592454.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5414..5758
                     /locus_tag="ACHAY1_000429"
     CDS             5414..5758
                     /locus_tag="ACHAY1_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8592455.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5771..6064
                     /gene="rpmA"
                     /locus_tag="ACHAY1_000430"
     CDS             5771..6064
                     /gene="rpmA"
                     /locus_tag="ACHAY1_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8592456.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6498..9305
                     /locus_tag="ACHAY1_000431"
     CDS             6498..9305
                     /locus_tag="ACHAY1_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8592457.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9496..9987
                     /locus_tag="ACHAY1_000432"
     CDS             9496..9987
                     /locus_tag="ACHAY1_000432"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8592458.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10090..11079
                     /locus_tag="ACHAY1_000433"
     CDS             10090..11079
                     /locus_tag="ACHAY1_000433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8592459.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11112..11915
                     /locus_tag="ACHAY1_000434"
     CDS             11112..11915
                     /locus_tag="ACHAY1_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8592460.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11936..12850
                     /locus_tag="ACHAY1_000435"
     CDS             11936..12850
                     /locus_tag="ACHAY1_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8592461.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12933..13307
                     /locus_tag="ACHAY1_000436"
     CDS             12933..13307
                     /locus_tag="ACHAY1_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8592462.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13446..13691
                     /locus_tag="ACHAY1_000437"
     CDS             13446..13691
                     /locus_tag="ACHAY1_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8592463.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13863..14927
                     /locus_tag="ACHAY1_000438"
     CDS             13863..14927
                     /locus_tag="ACHAY1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8592464.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15173..15598
                     /locus_tag="ACHAY1_000439"
     CDS             15173..15598
                     /locus_tag="ACHAY1_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8592465.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15591..16040
                     /gene="nusB"
                     /locus_tag="ACHAY1_000440"
     CDS             15591..16040
                     /gene="nusB"
                     /locus_tag="ACHAY1_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8592466.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16137..16991
                     /locus_tag="ACHAY1_000441"
     CDS             16137..16991
                     /locus_tag="ACHAY1_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8592467.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16992..18329
                     /gene="xseA"
                     /locus_tag="ACHAY1_000442"
     CDS             16992..18329
                     /gene="xseA"
                     /locus_tag="ACHAY1_000442"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8592468.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18334..18558
                     /locus_tag="ACHAY1_000443"
     CDS             18334..18558
                     /locus_tag="ACHAY1_000443"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8592469.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18560..19450
                     /locus_tag="ACHAY1_000444"
     CDS             18560..19450
                     /locus_tag="ACHAY1_000444"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8592470.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19459..20277
                     /locus_tag="ACHAY1_000445"
     CDS             19459..20277
                     /locus_tag="ACHAY1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8592471.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20384..20833
                     /locus_tag="ACHAY1_000446"
     CDS             20384..20833
                     /locus_tag="ACHAY1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8592472.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20851..22533
                     /gene="recN"
                     /locus_tag="ACHAY1_000447"
     CDS             20851..22533
                     /gene="recN"
                     /locus_tag="ACHAY1_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8592473.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22588..22704)
                     /locus_tag="ACHAY1_000448"
     CDS             complement(22588..22704)
                     /locus_tag="ACHAY1_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8592474.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22777..23733)
                     /locus_tag="ACHAY1_000449"
     CDS             complement(22777..23733)
                     /locus_tag="ACHAY1_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8592475.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23904..24272
                     /locus_tag="ACHAY1_000450"
     CDS             23904..24272
                     /locus_tag="ACHAY1_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8592476.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24442..24873
                     /gene="mraZ"
                     /locus_tag="ACHAY1_000451"
     CDS             24442..24873
                     /gene="mraZ"
                     /locus_tag="ACHAY1_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8592477.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24894..25853
                     /gene="rsmH"
                     /locus_tag="ACHAY1_000452"
     CDS             24894..25853
                     /gene="rsmH"
                     /locus_tag="ACHAY1_000452"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8592478.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25858..26259
                     /gene="ftsL"
                     /locus_tag="ACHAY1_000453"
     CDS             25858..26259
                     /gene="ftsL"
                     /locus_tag="ACHAY1_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8592479.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26260..28452
                     /locus_tag="ACHAY1_000454"
     CDS             26260..28452
                     /locus_tag="ACHAY1_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8592480.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28478..29440
                     /gene="mraY"
                     /locus_tag="ACHAY1_000455"
     CDS             28478..29440
                     /gene="mraY"
                     /locus_tag="ACHAY1_000455"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8592481.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29454..30815
                     /gene="murD"
                     /locus_tag="ACHAY1_000456"
     CDS             29454..30815
                     /gene="murD"
                     /locus_tag="ACHAY1_000456"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8592482.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30841..31929
                     /gene="murG"
                     /locus_tag="ACHAY1_000457"
     CDS             30841..31929
                     /gene="murG"
                     /locus_tag="ACHAY1_000457"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8592483.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31944..33164
                     /locus_tag="ACHAY1_000458"
     CDS             31944..33164
                     /locus_tag="ACHAY1_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8592484.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33320..34645
                     /gene="ftsA"
                     /locus_tag="ACHAY1_000459"
     CDS             33320..34645
                     /gene="ftsA"
                     /locus_tag="ACHAY1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8592485.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34668..35909
                     /gene="ftsZ"
                     /locus_tag="ACHAY1_000460"
     CDS             34668..35909
                     /gene="ftsZ"
                     /locus_tag="ACHAY1_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8592486.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35926..36603
                     /locus_tag="ACHAY1_000461"
     CDS             35926..36603
                     /locus_tag="ACHAY1_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8592487.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36617..37216
                     /locus_tag="ACHAY1_000462"
     CDS             36617..37216
                     /locus_tag="ACHAY1_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8592488.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37297..37518
                     /locus_tag="ACHAY1_000463"
     CDS             37297..37518
                     /locus_tag="ACHAY1_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8592489.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37567..38349
                     /locus_tag="ACHAY1_000464"
     CDS             37567..38349
                     /locus_tag="ACHAY1_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8592490.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38370..39077
                     /locus_tag="ACHAY1_000465"
     CDS             38370..39077
                     /locus_tag="ACHAY1_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8592491.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39339..42137
                     /gene="ileS"
                     /locus_tag="ACHAY1_000466"
     CDS             39339..42137
                     /gene="ileS"
                     /locus_tag="ACHAY1_000466"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8592492.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42186..43712)
                     /gene="zwf"
                     /locus_tag="ACHAY1_000467"
     CDS             complement(42186..43712)
                     /gene="zwf"
                     /locus_tag="ACHAY1_000467"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8592493.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43727..44374)
                     /locus_tag="ACHAY1_000468"
     CDS             complement(43727..44374)
                     /locus_tag="ACHAY1_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8592494.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44558..44887)
                     /locus_tag="ACHAY1_000469"
     CDS             complement(44558..44887)
                     /locus_tag="ACHAY1_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8592495.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45064..45792
                     /gene="sfsA"
                     /locus_tag="ACHAY1_000470"
     CDS             45064..45792
                     /gene="sfsA"
                     /locus_tag="ACHAY1_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8592496.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45808..46821
                     /locus_tag="ACHAY1_000471"
     CDS             45808..46821
                     /locus_tag="ACHAY1_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8592497.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46821..48098
                     /locus_tag="ACHAY1_000472"
     CDS             46821..48098
                     /locus_tag="ACHAY1_000472"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8592498.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48161..50863
                     /locus_tag="ACHAY1_000473"
     CDS             48161..50863
                     /locus_tag="ACHAY1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8592499.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51015..51332
                     /locus_tag="ACHAY1_000474"
     CDS             51015..51332
                     /locus_tag="ACHAY1_000474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8592500.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51362..51682
                     /locus_tag="ACHAY1_000475"
     CDS             51362..51682
                     /locus_tag="ACHAY1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8592501.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51790..53250
                     /locus_tag="ACHAY1_000476"
     CDS             51790..53250
                     /locus_tag="ACHAY1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8592502.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53318..53539
                     /locus_tag="ACHAY1_000477"
     CDS             53318..53539
                     /locus_tag="ACHAY1_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592503.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53570..53752
                     /locus_tag="ACHAY1_000478"
     CDS             53570..53752
                     /locus_tag="ACHAY1_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8592504.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53752..54165
                     /locus_tag="ACHAY1_000479"
     CDS             53752..54165
                     /locus_tag="ACHAY1_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8592505.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54288..55469
                     /locus_tag="ACHAY1_000480"
     CDS             54288..55469
                     /locus_tag="ACHAY1_000480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592506.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55540..55704)
                     /locus_tag="ACHAY1_000481"
     CDS             complement(55540..55704)
                     /locus_tag="ACHAY1_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592507.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55858..55931
                     /locus_tag="ACHAY1_000482"
     tRNA            55858..55931
                     /locus_tag="ACHAY1_000482"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55892..55894,aa:Arg,seq:cct)
     gene            complement(56000..57139)
                     /locus_tag="ACHAY1_000483"
     CDS             complement(56000..57139)
                     /locus_tag="ACHAY1_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8592508.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57311..57445
                     /locus_tag="ACHAY1_000484"
     CDS             57311..57445
                     /locus_tag="ACHAY1_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592509.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57438..58073)
                     /locus_tag="ACHAY1_000485"
     CDS             complement(57438..58073)
                     /locus_tag="ACHAY1_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592510.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58186..58821)
                     /locus_tag="ACHAY1_000486"
     CDS             complement(58186..58821)
                     /locus_tag="ACHAY1_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8592511.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58855..59316)
                     /locus_tag="ACHAY1_000487"
     CDS             complement(58855..59316)
                     /locus_tag="ACHAY1_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8592512.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59446..59877)
                     /locus_tag="ACHAY1_000488"
     CDS             complement(59446..59877)
                     /locus_tag="ACHAY1_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8592513.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59895..60215)
                     /locus_tag="ACHAY1_000489"
     CDS             complement(59895..60215)
                     /locus_tag="ACHAY1_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8592514.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60514..61290
                     /locus_tag="ACHAY1_000490"
     CDS             60514..61290
                     /locus_tag="ACHAY1_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8592515.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61305..61508
                     /locus_tag="ACHAY1_000491"
     CDS             61305..61508
                     /locus_tag="ACHAY1_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592516.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61524..61862
                     /locus_tag="ACHAY1_000492"
     CDS             61524..61862
                     /locus_tag="ACHAY1_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8592517.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61849..62028
                     /locus_tag="ACHAY1_000493"
     CDS             61849..62028
                     /locus_tag="ACHAY1_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592518.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62071..62541)
                     /locus_tag="ACHAY1_000494"
     CDS             complement(62071..62541)
                     /locus_tag="ACHAY1_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592519.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62628..63326
                     /locus_tag="ACHAY1_000495"
     CDS             62628..63326
                     /locus_tag="ACHAY1_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8592520.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63504..63845
                     /locus_tag="ACHAY1_000496"
     CDS             63504..63845
                     /locus_tag="ACHAY1_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592521.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63838..64509
                     /locus_tag="ACHAY1_000497"
     CDS             63838..64509
                     /locus_tag="ACHAY1_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8592522.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64515..65207
                     /locus_tag="ACHAY1_000498"
     CDS             64515..65207
                     /locus_tag="ACHAY1_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8592523.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65204..65953
                     /locus_tag="ACHAY1_000499"
     CDS             65204..65953
                     /locus_tag="ACHAY1_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592524.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65965..66234
                     /locus_tag="ACHAY1_000500"
     CDS             65965..66234
                     /locus_tag="ACHAY1_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592525.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66240..66404
                     /locus_tag="ACHAY1_000501"
     CDS             66240..66404
                     /locus_tag="ACHAY1_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592526.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66397..66696
                     /locus_tag="ACHAY1_000502"
     CDS             66397..66696
                     /locus_tag="ACHAY1_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592527.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66696..66998
                     /locus_tag="ACHAY1_000503"
     CDS             66696..66998
                     /locus_tag="ACHAY1_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8592528.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            67038..67403
                     /locus_tag="ACHAY1_000504"
     CDS             67038..67403
                     /locus_tag="ACHAY1_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592529.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67893..68033
                     /locus_tag="ACHAY1_000505"
     CDS             67893..68033
                     /locus_tag="ACHAY1_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592530.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            68030..68212
                     /locus_tag="ACHAY1_000506"
     CDS             68030..68212
                     /locus_tag="ACHAY1_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592531.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68225..68419
                     /locus_tag="ACHAY1_000507"
     CDS             68225..68419
                     /locus_tag="ACHAY1_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592532.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT        22850 a        11442 c        14396 g        19884 t
ORIGIN      
        1 attagaaaca tataattatt aaagggaaac atttttacag ctataatttg aaaaaattta
       61 tcgttattat atgattaaga aggattgaat aaagaaagaa gttgaaaacg atacctgatt
      121 acaaaaaaat aacttttata agttctataa cgttgattat atttttaatt cttattcttc
      181 tcgtcgtctt ggttccccga atttttggtt tgactcctta tattattgag gacaaagaaa
      241 tgagtccata ttatgcacaa ggaaatctga tatatgtacg accgaaaaat acaaatgaga
      301 ttttagtcgg agatattatt acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc
      361 gcgtaatagc cgttgaaaac aaccacgaag atttttatac aaaagctgat acaaagactt
      421 atattgagcc tggagtagta aaaaagagaa atattatcgg tagtcccatc tttcagattc
      481 catatattgg actagtcctt tcaaaaacgg catttgcatg gattcagggg ttattcttta
      541 cgattgcttt ttgcctaaca ggaatcacag catggaatac ctttgtagag ttgaaaaaga
      601 agcggagacg aacacagaaa tttttgtaag aatgttaaaa acataaatgt attccagaga
      661 tccaacgact cttctgaaca tgaaacacga gaaaacactc atgcagcttg tagatttagt
      721 cagttgttcg tataatgtgt gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt
      781 cctgtagtca aagaagaact attggatttt atgcgtaccc agcaaaaaaa attaccgggt
      841 gagctaggca aactggaaga agaagctcat gtagcagaag taccagttat tccccatgaa
      901 acagttgtat ttttgaaatt tttacttgga cagcttcagc ctgaacgaat cttggagatc
      961 ggagcagcta tcggtttttc atcaagtgtc atggccacga caatcggaga aaacgctcat
     1021 gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt
     1081 ctaggactga cagataaagt gactttgctt gaaggtcaag cagcagatat cttgccggag
     1141 ttgtctggtc cgtatgactt tatttttatg gatagtgcaa aatcaaaata tattgaattt
     1201 ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc
     1261 caaggaggaa cgatcttgct cccagatgaa gagattcccc gtggcaaacg agctatccat
     1321 cgtaaattaa atgaatttct tcgtgttgtg atggatcatc ccgatttgac atcgacaatc
     1381 ttgccattag gtgatggtgt gatcttaatg acaaaggaat cagaaacaat cgagttgtga
     1441 tacatgatta tggcagcctg tagataatcg attgaatcga gggctcaatt cgaacttgat
     1501 gtaaaagttt ctatatttgt aagtcaatta aaggatacag gaaatctaaa taaacaggag
     1561 tagcatgaag tcaatgtagc tcctgttttc aattgataac cttagaacag ctgaaggaga
     1621 atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa ataattcatt atttgctaat
     1681 taaataacta tttcgtattg acagaagcaa tttaattatc tataattatt gatgttccgc
     1741 tgattcttgt catagtagct cagctggata gagcatccgc cttctaagcg gacggtcggg
     1801 ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt
     1861 tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca aataaaataa aaaaacatcc
     1921 ttatcgagta caattataag gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat
     1981 atgacgatac tatctagaat tttagtgact ctagttgcaa ttgaattttt ttatatcatg
     2041 tatatcgaaa cggtgagaac tgattctgac acgacaagta gggtttttaa aatgagtaaa
     2101 gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga accaaggagt gtacaatgga
     2161 ctattaggag tgggactact ttatggagct tatctttctt ccgcatctaa agaaatcaca
     2221 agtatgctat taatatctat tttttttgtt gcattatatg gcagtttgac tagcgatcgt
     2281 tggattttac tcaaacaagg tgggctagct ctgttggcat tgctttctat tttcttataa
     2341 atatagaaaa aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc
     2401 atgaaatatg atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat
     2461 aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa
     2521 gatttcagaa agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct
     2581 tggcttttgg ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg
     2641 ccggtatcgt tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg
     2701 cgatggcagc tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg
     2761 ctactttttt agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga
     2821 cttcttttat cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg
     2881 cattaggtca gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa
     2941 aactaagtag ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag
     3001 ggctagtcat tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt
     3061 cgctgctggc tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa
     3121 ctgtgggtaa gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta
     3181 gcttttctgc cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga
     3241 ttgaaagctt gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca
     3301 atcaagaatt aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc
     3361 ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat
     3421 tagctggtat gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga
     3481 tgtcacagtt tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga
     3541 atgaatggga aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt
     3601 ttatccttac gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg
     3661 tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa
     3721 cgatcgattg gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag
     3781 tgtatattag tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag
     3841 aactaaatca tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga
     3901 cggcacgtgc agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta
     3961 tctatacttc cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat
     4021 tcacgcatca acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg
     4081 caattagcca ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gattttatgg
     4141 tcttgtctgt tctttttaat attgctgtaa aagtgaagct agtacttgcc agtcttgatg
     4201 gataacaggt acaagggagc gattgtagaa aatagctgct ggatgaaaaa gtggaagaat
     4261 tcgatagttt ttttctgacc attcgtaggc atccttttgt tctgttaatt gtaagacagg
     4321 ccctgtatag agttgaccat gatttttggt aaccgtatat tctttgccaa gtaaacgttg
     4381 aagaccaatg tttccaagtg tcgcaattaa ttttggctgg accttcatca gttcgtaatc
     4441 caatattggt gcatgcgcaa atacttcgga acgagtaggt gtccgatttg gataaacagt
     4501 ttctgactgt tgtgtccgtt tattgatccg atgagtcacg cgatacgggc ggctccttac
     4561 agcactggtg atatacacct cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa
     4621 ttcttttcca gcttgaccgc taaaaggaac atgcgtatca atctctgttt tccctggggc
     4681 ttctcccacg agcatcagtt taggatgaag aggtccttgc ccagcgataa atccttctac
     4741 aggacggccg ttcattctta aagaagcttc ttgtatcagt tcttttgggt attccattta
     4801 tgctcctcct ttttctttta ttataagaat aaaagaaaca caatgcgact attttatttg
     4861 gtagaactgt gaggatagaa aagcagaagt attgcttgaa tttttgtttt actttttgat
     4921 tttaaattta gttgactaac ggttgctgtt ctggtaagct agtactgttg taattgtgga
     4981 ctccacagct acaaccgctc agaacaagta ataagcacag catatgctgt cacttcggat
     5041 ggcgagtcta agtatatcag aaggaggtgc tttatagcat gtacgcaatt attaaaactg
     5101 gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg
     5161 aagcaggaga aaaagttgtt tttgacgaag ttatcttagt aggcggagaa tctacaaaag
     5221 taggcgctcc aactgttgca ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac
     5281 aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc
     5341 atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa
     5401 agaaggccta tatatgatca aaggaacatt taagcgaaat gacgcaggtc agatcgtttc
     5461 attcactttg accgggcatg ctgatgctgg tccttatgga agtgatatcg tttgtgcggg
     5521 tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc tcattagccg gttttgagcc
     5581 gattgttgaa atgaatgaag aagaaggcgg ttatctttat accgaagtca cttcaggaat
     5641 gacacaagaa caaaacaata ttgctgagat tctattagaa aatcttttat taggattaca
     5701 atcgatagaa gctgaaaact cagaatatat ccaaattaaa acaattaatg ataaatagga
     5761 ggtgcagacc atgttgttat ctatgaatct acaattattt gcccacaaaa aaggtggcgg
     5821 ttctacatct aacggccgtg actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg
     5881 acaaacagtt acaggtggat caattcttta ccgtcaacgc ggtacaaaaa tctatccagg
     5941 tgtaaacgtg ggtatcggtg gagacgacac tttatttgca aaagttgatg gcgtagtacg
     6001 tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc
     6061 ttaattgaat cttatgatta aagaggttgt gacaagactt ttgtcacagc cttttttgtt
     6121 tataaaaata aaagttgtag tggaggctac gaagtttact ttgtcttttt acacttaata
     6181 tttttttgcc aatttggaat caggatgttt tgataactat cgttttcaat tgatggaagc
     6241 tatattgttt gctatcagtg aaataattat atggtaaaca gtgttctttt tcttgtttcc
     6301 attataaaaa tcgtatcggg atttttcgtt cttttaaatt gaacatttct atctatccgc
     6361 gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa acagaaataa atacatagta
     6421 ttatgttttg aagcctaagt acaatagtct atagaaaagg agcgatacca ttggcaaaac
     6481 gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat
     6541 atgatcaagg agtaacaaca caggaaattg ctgaagtgtt agggattcag cgaacaaata
     6601 gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact gataaagaca gacggacgac
     6661 cagtacgtta catatatcaa acactcgtta cgcacgtaaa gccagttaca aaacatgtag
     6721 tcagttacaa agaaacgccg gtctctgaag aaaaaaatac actccaaata gatactaagg
     6781 atatctttgc aaaaatcatt ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa
     6841 aagctgcgat tttgtatccg ccaagaggat taaattgttt gattacagga cctaccggat
     6901 caggaaagac ttattttgca cacgcgatgt atcattttgc aaaagcgcat catgtaatag
     6961 atgaggaaac ggaactgatt gttttcaatt gtgctgatta tgccaataac cctgagcttt
     7021 tgatgagtca tttatttggg tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa
     7081 tggggattat cgatcaagcg gatggcggta tgctgttttt ggatgagatt caccggttgc
     7141 cgcctgaagg tcaagaaatg atcttttact ttatggatca tggaacttac agtcgtcttg
     7201 gggaaacaac gaaatcccat gaagcaaatg tacggatcgt aggtgcgacc acagaagacc
     7261 caggttcttc tctattagaa acttttgttc ggcgtatccc aatcaatatc aaacttccgg
     7321 cttttgaaaa acgtcctgca aatgagaaag tcgatttagt aaaaatcatg attgcccacg
     7381 aagcaaaccg cacgcagcga aagatttctt tgacagaaga tgtagcaaaa gcattgatcg
     7441 gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa tatccagtta gtatgtgcac
     7501 gaggatttct aaatcatatg caatcacccg aaatctcgat cacaatcgat gatttgacag
     7561 aagggattcg tagcggattg attcaattag caagcaaccg cagtgcaatg gcagagcttt
     7621 ctaaaatatt ggagccaaag ataactgtag caccaaatga tacgatcatg aaaatccaat
     7681 cggactccta tgagttacct tataatctat atgatatcat cggagataaa gcagctttac
     7741 tgaagtcgga tggtttggat caagaagcaa tcaatcattt catttctaca gatatcaaca
     7801 tccatctgaa gtctttctat aaagaccatg gtttttcttt taatgcagac agcaaattag
     7861 cagaatttgt agatccgaaa atcatagaag tgaccaatca gatttatgta atggtcaaaa
     7921 gatctttacc atatgagttt cagcaaaact ttatttatgc tatgagcttg catatcagtt
     7981 cttttttgaa aaaaatcaac ttaggagaag aacggcatac gaatgataac atccgtgaga
     8041 tggcttttga ttttccagat gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa
     8101 cgtattttca agtggagatt ccagaaagtg aagactatta cttgaccgtt ctactcgtct
     8161 cgttacgggc aaatcaagca agcggcagaa tcggtgtagt cgttgcagct cacggaaaca
     8221 gcacagcaag cagtatggtc caagttgtcc aacagttgtt agatgcagag aacgtacgag
     8281 cagtagacat gcctttggat atggatccga aaacagcatt ggaccgaatt gaacgcaatg
     8341 tacaagaagt agatgaagga agcggggtca tactcttagt agatatgggg tcccttgcgt
     8401 cgtttaattc gcaaattcaa agagacactg gaattccagt acgtactgtt gatatggtaa
     8461 caacttctct agttttggaa actgtacgaa aggtctcagt attagggaca gatctagata
     8521 tgttgcatga atcattgaaa aattttcgag gttacgcaga aatatcttct gtagaatctg
     8581 ttcaggaagc aaaacctatt cataagaaaa aagctgtttt ggctatctgc gcatctggcg
     8641 aaggtacagc acaaagaatc aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa
     8701 cagaattaga agttctagct ttgtcgattg tggaagtgaa agaagaactg ccagttatcc
     8761 aaaaagaata tcagatcata gctactactg ggattactga tttgaatatc caagccccat
     8821 ttattccttt agaacgtttt attgatcaaa atatcgaagt cattttggat cagttgctga
     8881 tggaatcaga attggaggaa actgaattca tctccctaga tgaagagtca gctaaaaata
     8941 cgtgtgttga gtttatctct gataacttca tctttattaa tggtagtaag ttaatcgatc
     9001 caatgtggca attttctaca caaatctctc agacgacggg aatcggcgat gaagaatatg
     9061 gattcaaaat caacctagtg atgcacacag caggaatgat cgaacgtatt attcggaatg
     9121 aaccattgac tgtggaagaa aacgaattga caaatacaac gaatgatcct ctttatagcc
     9181 aattggcagc ctcagttgtt ctgttggaag atcagataaa agtaaaagtg ccgatagaag
     9241 aaatgtatta tctgctacgt ctagtccata atcagttaga taaaaaagaa tacactgtgc
     9301 catgaataca caaaaacgat acacttttat agtgtatcgt ttttgtgtat tggttaagaa
     9361 tcctttaaat tcaattagtg tatcgtaaaa aagttggcac gctttttgct tatagtagtg
     9421 tgtgctagga ggtggttgtt cacacagaaa tgataggggt ttctgtgaag tagtgaggaa
     9481 gaaggagttt tgcagatgga tattcgattg gcacgtatag atgatcgttt aatccacggg
     9541 caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat
     9601 gtagtcgctc aagataaatt gagaaagttt ctactaaaag aagctgcacc gccggggatc
     9661 acagcaaatg taataaccgt gcagaaaatg attcaaatct atcatagtgg attattatca
     9721 cgagcaaaag tgatgctttt attcaccaat ccccaagatg ttgaagcggt tgtccgctgc
     9781 ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg caaatcaatg
     9841 attaccaatt ttgtttcagt aaatcaacaa gatattgatt cctttcgtta cttgaacagt
     9901 caagaagtgg agttagagat tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa
     9961 ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt caaataattc
    10021 tgttgtaatg gttagcaatt attaatattt ttgaatttgt tacaacaaaa gtaaatagga
    10081 ggtataaata tggtagggat tatcctagca agtcatggac aatttgctga aggaatctta
    10141 caatccggtt caatgatctt tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca
    10201 agtgaaggtc cagatgactt gagagccaaa ttggaagaag cggttgcttc ttttgataat
    10261 caagacgaag tgttattctt agttgattta tggggcggaa caccgttcaa tcaatcaaac
    10321 acattattcg aagagcacaa agataaatgg gcaatcgtca gcggtttgaa tttaccaatg
    10381 ttgattgaag cctacgcatc acgcttttca atggaatctg ctcatgaaat tgcagcacac
    10441 atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa
    10501 gcaccgaaag cagcagttga agacgcacaa cctaaaggag cacttccaga aggaactgtg
    10561 gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg actcacgtct gttacatgga
    10621 caagttgcga cagcttggac aaaagcagtg caaccaaacc gtattatcgt tgtttcagat
    10681 gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta
    10741 aaagcaaatg ttattcctat tagcaaaatg atcgaagtag caaaagaccc tcgttttgga
    10801 aataccaaag cattgctttt atttgaaaat cctgaagacg tattgacagc tgttgaaggt
    10861 ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt
    10921 gtaagtaaag tattgtctat gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa
    10981 aaaggtgtga aattcgatgt acgtaaagta cctaatgatt cacgcgataa catggatgat
    11041 attttgaaaa aagcaaaagc tgaattagca aaagcgtaat aaataatatt aaaatttata
    11101 ggaggcttat catgtctatt atttcaataa ttttagtcgt attagttgcc tttctagctg
    11161 gtatggaagg tattcttgat gaatttcaat ttcatcaacc actagtggca tgtacattga
    11221 tcggtttagt taccggtaac ctcgaagctg gtatcgtact tggcggtacg cttcagatga
    11281 tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc agatgctgca ttagcatcag
    11341 tagcatcagc aattatttta gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga
    11401 tcgcgattgc cgttcctttg gctgttgcag ggttgttctt aacaatgatc gtacgtacaa
    11461 tcgctgttcc aatcgttcat ttgatggatg cggctgctga agaaggaaac atccgtaagg
    11521 ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag
    11581 cagcgttgct atttatccca gcacaaactg tgcaatcatt ccttgaatca atgccagcat
    11641 ggttaacaga tggtatggct atcggtgggg gaatggtcgt agctgtaggt tacgctttgg
    11701 taatcaacat gatggctaca aaagaagtat ggccattctt cgttattggt ttcgttgtag
    11761 ctgcaatttc tcaattaaca ttgattgctc taggtgcttt aggtgttgca ttagctctta
    11821 tttacttgaa cctttctaaa atgggtggtt catcaaataa tggaggcgga agcaactctg
    11881 gtgacccttt aggcgacatt ttgaatgact attaatcttg aaggaggaaa ataaaatggc
    11941 agaagaaaaa atcaaattat caaaaaaaga tcgtttagct gttgcgtggc gctctacctt
    12001 cattcaaggt tcatggaact acgaacgtat gcaaaacggt ggctgggcat tttctatgat
    12061 tccagcaatc aaaaaattat acaaaacaaa agaagaacgt tctgcagcat taaaacgtca
    12121 cttggaattc tttaatacac acccatacat tgcttctcca attttagggg ttacattggc
    12181 tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc aaggggttaa
    12241 agtaggtatg atgggtcctt tagctggtgt tggtgaccca gtgttctggt ttacagtacg
    12301 tccaatgcta ggtgcactag gtgcttcatt agctatggga ggaaatatcc ttggaccaat
    12361 catcttcttc ttaggttgga acattattcg ttgggcattc atgtggtata cacaagaatt
    12421 tggctacaaa gctggttcta aaattactga tgacctttct ggcggattgt tacaagacgt
    12481 aacaaaaggt gcttcaattt tagggatgtt cgtattagct gcccttgtac aaagatgggt
    12541 atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg cttacatcga
    12601 atgggataaa ttgccttcag gtggtgaagg aatcaagagt gcatttgaac aagttaataa
    12661 tggcttagcc ctttctcctg aaaaggtaac aacattacaa gacaacttag accaattgat
    12721 tcctggttta gctgcattag gattaacatt gttatgtatg tggttgctta agaaaaaggt
    12781 ttctccaatc gttatcatcc ttggtttatt cgtagtcggt gttgttggac acgtcatcgg
    12841 acttttataa tccataaaaa tatagctcgg gcgttgtccc gagctttttt tatatagtat
    12901 actgttcata aacaaatatc tggagggaga aaatggttca atcaatcaat acaaaagtag
    12961 acttggtaat tgatgctact tcgtttacgg gtctgacaga ttacgggcaa atcatgatag
    13021 gtaataatgg ttttgagttt taccatgcgc gagatagacg caaatatatc caaattccat
    13081 gggaagaagt ggattatgta ctagcatcag tcatgctaaa gggaaaatgg attcctcgat
    13141 atgccattca aacgaaaaaa aatggtacat acacattttc ttccaaagaa cctaaaaaag
    13201 tgcttcgtgc gatcagagaa tatgtagatg aagatagaat ggtacaatca ttgagtttct
    13261 ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa
    13321 cttctaagag gaggttttct ttattttttt actttgcaga agaaaatatg aaatagcaaa
    13381 accaattatt tgtccccttt aaatcccata tgctaaaatt gattagcaaa gaaggaggtt
    13441 atattatgag ttggttatgg acattgattg ttggagccgt gatcggagca attgcaggtg
    13501 cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa tatcattgct ggactagtcg
    13561 gttcaagtat tggacaagcg attttcggcg aatggggacc aaaagctgca ggtatggcac
    13621 ttattccatc tattttaggc gcagtgattt tggtagcagt tgtctcattt ttctttggaa
    13681 aacgatcata agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc
    13741 ctttttggct ttatttattt aaaataaaaa gaagttttcc ggaaattttt atttaactgt
    13801 cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga gggagtttca
    13861 ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat ttggattcat
    13921 ttttggttac cagtccgtac aatcttcgtt acttaacgaa ctttacaggt acgacaggat
    13981 tagctgttat tactttggaa aaagcatttt ttattactga ttttcgttat accgagcagg
    14041 cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt tttgatgagg
    14101 tagcaaactt agtacgcaaa gaagaactgc actcattagc ttttgaggaa acaacagtct
    14161 catttttaga atattcggtt ttagaagaaa tcatcgatgc ggaattagta cctgtttcac
    14221 agatgatcga agaactcaga gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag
    14281 catgtcatat tgcggatatg gcatatgatc acatcctgaa aatgatccaa ccgggcatga
    14341 ctgagattga agtcgcaaat caactagact tttatatgcg ttctcttggc gcttcaggtg
    14401 tttcttttga tacgatcgtt gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa
    14461 gcaaaaaaat catcgaacaa ggtgacttga tcacattgga ctttggttgc tattacgaag
    14521 ggtacgtttc cgatatgaca cgtacattcg ctattggtga tcctggggaa aaattaaaag
    14581 aaatttatca aatagtctta gatgctcaat tagcagttat tgatgctgca aaaccaggaa
    14641 tgactggtgt ccaattagat gcagttgcta gagattatat ttcaaaacat ggttacgggg
    14701 aagcatttgg acattctaca ggacatggga tcggtttaga gatccacgaa ggaccaaacg
    14761 tttcttctcg tgcagaaaag caatttgtac caggaaatgt gatcactgat gaaccaggga
    14821 tctacttacc aggaatcggt ggagtacgta ttgaggatga cttgttgatc acagaagatg
    14881 gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat cttatagttt catcaataga
    14941 agaagtaagc gtaaaaatca gtgttcacag actttgttat agaaaatcac tcgatttaat
    15001 gatacactat agaagaactt ttaagctgta agagataata aagttgggtg ttttctccaa
    15061 ttcatagcag tttgcagctg atcggcggca gttttcttac cacgtgatca ctatgccgca
    15121 tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg aggaataaat aaatggcaga
    15181 tgaaaaaaat ttgattttaa acgcaaacca agatttagga gagatcgtga tcgctcctga
    15241 agtcatcgaa gtaattatcg ggattgcagc ttctaaagta gaaggcgtat acggaatgcg
    15301 cggaactttt gccagcaatg tcactgaatt attaggacgt gcagctcacg gaaaaggtgt
    15361 ttacttagca aacgaggaag atggcttgaa agtagatctt tactgttatc tagaatatgg
    15421 tgtttcagtt cctaaagtag caatggaaat gcaggaacgg gtcagccagc aagtcctttt
    15481 catgacggat atcgatttat ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa
    15541 attagaacaa ccaacaatcg aagaactttt tgaagacgaa gaggaagaga atgaataaag
    15601 aattatcaag acacgagatt cgggaaatgg cattgcaggc tttatttcca ttagacttca
    15661 atgcagacct aacaaaagaa gatgcgattt tcaacgcaat cgaattagac caccgcgaca
    15721 tgatcaacga agatgaatca gagtttgttc ctgtttatct tgatacgcta gttggcggtg
    15781 tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa acatttaaaa aacaattggc
    15841 acatcaatcg gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt tttgaaatga
    15901 cttatgtgac agatgtacca gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa
    15961 catttagtga tgaccgttca cgtaaattcg tcaacggtgt tttatccaat gtattgaaag
    16021 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
    16081 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
    16141 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
    16201 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
    16261 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
    16321 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
    16381 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
    16441 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
    16501 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
    16561 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
    16621 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
    16681 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
    16741 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
    16801 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
    16861 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
    16921 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
    16981 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
    17041 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
    17101 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
    17161 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
    17221 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
    17281 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
    17341 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
    17401 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
    17461 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
    17521 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
    17581 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
    17641 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
    17701 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
    17761 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
    17821 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
    17881 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
    17941 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
    18001 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
    18061 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
    18121 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
    18181 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
    18241 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
    18301 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
    18361 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
    18421 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
    18481 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
    18541 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
    18601 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
    18661 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
    18721 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
    18781 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
    18841 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
    18901 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
    18961 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
    19021 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
    19081 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
    19141 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
    19201 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
    19261 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
    19321 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
    19381 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
    19441 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
    19501 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
    19561 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
    19621 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
    19681 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
    19741 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
    19801 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
    19861 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
    19921 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
    19981 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
    20041 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
    20101 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
    20161 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
    20221 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
    20281 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
    20341 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
    20401 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
    20461 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
    20521 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
    20581 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
    20641 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
    20701 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
    20761 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
    20821 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
    20881 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
    20941 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
    21001 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
    21061 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
    21121 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
    21181 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
    21241 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
    21301 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
    21361 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
    21421 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
    21481 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
    21541 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
    21601 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
    21661 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
    21721 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
    21781 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
    21841 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
    21901 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
    21961 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
    22021 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
    22081 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
    22141 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
    22201 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
    22261 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
    22321 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
    22381 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
    22441 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
    22501 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
    22561 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
    22621 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
    22681 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
    22741 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
    22801 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
    22861 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
    22921 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
    22981 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
    23041 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
    23101 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
    23161 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
    23221 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
    23281 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
    23341 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
    23401 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
    23461 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
    23521 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
    23581 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
    23641 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
    23701 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
    23761 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
    23821 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
    23881 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
    23941 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
    24001 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
    24061 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
    24121 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
    24181 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
    24241 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
    24301 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
    24361 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
    24421 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
    24481 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
    24541 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
    24601 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
    24661 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
    24721 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
    24781 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
    24841 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
    24901 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
    24961 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
    25021 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
    25081 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
    25141 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
    25201 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
    25261 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
    25321 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
    25381 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
    25441 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
    25501 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
    25561 ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg
    25621 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
    25681 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
    25741 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
    25801 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
    25861 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
    25921 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
    25981 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    26041 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    26101 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    26161 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    26221 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    26281 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    26341 attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc
    26401 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    26461 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    26521 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    26581 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    26641 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    26701 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    26761 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    26821 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    26881 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    26941 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    27001 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    27061 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    27121 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    27181 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    27241 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    27301 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    27361 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    27421 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    27481 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    27541 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    27601 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    27661 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    27721 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    27781 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    27841 gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg
    27901 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    27961 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    28021 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    28081 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    28141 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    28201 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    28261 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    28321 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    28381 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    28441 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    28501 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    28561 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    28621 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    28681 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    28741 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    28801 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    28861 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    28921 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    28981 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    29041 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    29101 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    29161 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    29221 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    29281 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    29341 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    29401 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    29461 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    29521 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    29581 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    29641 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    29701 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    29761 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    29821 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    29881 cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    29941 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    30001 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    30061 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    30121 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    30181 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    30241 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    30301 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    30361 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    30421 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    30481 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    30541 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    30601 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    30661 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    30721 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    30781 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    30841 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    30901 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    30961 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    31021 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    31081 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    31141 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    31201 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    31261 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    31321 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    31381 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    31441 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    31501 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    31561 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    31621 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    31681 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    31741 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    31801 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    31861 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    31921 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    31981 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    32041 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    32101 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    32161 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    32221 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    32281 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    32341 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    32401 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    32461 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    32521 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    32581 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    32641 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    32701 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    32761 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    32821 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    32881 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    32941 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    33001 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    33061 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    33121 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    33181 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    33241 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    33301 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    33361 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    33421 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    33481 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    33541 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    33601 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    33661 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    33721 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    33781 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    33841 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    33901 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    33961 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    34021 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    34081 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    34141 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    34201 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    34261 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    34321 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    34381 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    34441 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    34501 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    34561 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    34621 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    34681 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    34741 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    34801 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    34861 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    34921 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    34981 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    35041 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    35101 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    35161 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    35221 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    35281 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    35341 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    35401 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    35461 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    35521 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    35581 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    35641 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    35701 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    35761 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    35821 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    35881 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    35941 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    36001 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    36061 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    36121 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    36181 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    36241 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    36301 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    36361 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    36421 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    36481 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    36541 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    36601 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    36661 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    36721 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    36781 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    36841 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    36901 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    36961 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    37021 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    37081 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    37141 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    37201 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    37261 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    37321 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    37381 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    37441 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    37501 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    37561 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    37621 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    37681 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    37741 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    37801 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    37861 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    37921 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    37981 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    38041 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    38101 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    38161 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    38221 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    38281 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    38341 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    38401 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    38461 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    38521 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    38581 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    38641 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    38701 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    38761 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    38821 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    38881 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    38941 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    39001 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    39061 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    39121 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    39181 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    39241 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    39301 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    39361 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    39421 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    39481 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    39541 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    39601 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    39661 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    39721 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    39781 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    39841 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    39901 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    39961 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    40021 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    40081 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    40141 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    40201 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    40261 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    40321 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    40381 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    40441 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    40501 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    40561 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    40621 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    40681 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    40741 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    40801 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    40861 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    40921 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    40981 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    41041 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    41101 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    41161 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    41221 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    41281 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    41341 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    41401 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    41461 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    41521 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    41581 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    41641 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    41701 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    41761 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    41821 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    41881 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    41941 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    42001 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    42061 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    42121 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    42181 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    42241 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    42301 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    42361 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    42421 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    42481 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    42541 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    42601 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    42661 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    42721 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    42781 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    42841 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    42901 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    42961 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    43021 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    43081 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    43141 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    43201 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    43261 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    43321 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    43381 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    43441 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    43501 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    43561 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    43621 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    43681 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    43741 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    43801 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    43861 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    43921 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    43981 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    44041 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    44101 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    44161 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    44221 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    44281 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    44341 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    44401 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    44461 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    44521 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    44581 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    44641 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    44701 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    44761 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    44821 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    44881 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    44941 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    45001 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    45061 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    45121 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    45181 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    45241 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    45301 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    45361 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    45421 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    45481 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    45541 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    45601 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    45661 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    45721 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    45781 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    45841 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    45901 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    45961 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    46021 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    46081 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    46141 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    46201 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    46261 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    46321 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    46381 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    46441 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    46501 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    46561 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    46621 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    46681 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    46741 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    46801 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    46861 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    46921 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    46981 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    47041 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    47101 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    47161 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    47221 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    47281 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    47341 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    47401 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    47461 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    47521 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    47581 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    47641 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    47701 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    47761 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    47821 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    47881 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    47941 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    48001 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    48061 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    48121 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    48181 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    48241 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    48301 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    48361 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    48421 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    48481 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    48541 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    48601 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    48661 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    48721 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    48781 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    48841 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    48901 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    48961 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    49021 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    49081 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    49141 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    49201 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    49261 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    49321 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    49381 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    49441 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    49501 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    49561 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    49621 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    49681 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    49741 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    49801 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    49861 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    49921 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    49981 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    50041 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    50101 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    50161 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    50221 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    50281 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    50341 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    50401 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    50461 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    50521 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    50581 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    50641 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    50701 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    50761 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    50821 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    50881 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    50941 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    51001 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    51061 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    51121 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    51181 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    51241 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    51301 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    51361 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    51421 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    51481 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    51541 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    51601 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    51661 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt
    51721 gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg
    51781 aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca
    51841 aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc
    51901 gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa
    51961 cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg
    52021 aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg
    52081 atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct
    52141 ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca
    52201 gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt
    52261 ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat
    52321 catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt
    52381 ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca
    52441 gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt
    52501 atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa
    52561 tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca
    52621 gtatttgttc aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg
    52681 ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat
    52741 aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg
    52801 tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc
    52861 ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta
    52921 tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta
    52981 ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga
    53041 tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta
    53101 ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca
    53161 ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa
    53221 atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt
    53281 ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat
    53341 gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac
    53401 atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt
    53461 tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt
    53521 tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa
    53581 cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc
    53641 gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta
    53701 tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc
    53761 acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt
    53821 ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag
    53881 tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa
    53941 aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa
    54001 atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat
    54061 ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg
    54121 agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg
    54181 tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat
    54241 ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag
    54301 taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta
    54361 atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga
    54421 attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc
    54481 cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc
    54541 cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc
    54601 gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct
    54661 atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt
    54721 ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa
    54781 tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact
    54841 ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg
    54901 ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg
    54961 cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc
    55021 acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt
    55081 atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg
    55141 tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc
    55201 tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg
    55261 aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa
    55321 gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc
    55381 aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc
    55441 aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca
    55501 ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt
    55561 cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat
    55621 tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac
    55681 tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc
    55741 tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt
    55801 ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt
    55861 cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc
    55921 agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt
    55981 gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga
    56041 ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag
    56101 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
    56161 gtaaaataga tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta
    56221 attcttttaa ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt
    56281 ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt
    56341 tttgattgat tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc
    56401 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
    56461 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag
    56521 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
    56581 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
    56641 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
    56701 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
    56761 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
    56821 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
    56881 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
    56941 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
    57001 attctaattc gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta
    57061 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
    57121 gtttgactaa tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata
    57181 aagaagccca ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa
    57241 ggatgtgcta tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga
    57301 ggtgtttatt atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt
    57361 atttgttgat tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt
    57421 catattagaa agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat
    57481 attttgtagc ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt
    57541 tctaacagac caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag
    57601 ccagacatgc tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac
    57661 aattgaatct caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc
    57721 catgaagcgt tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt
    57781 ttattttgtg cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt
    57841 tggtccttgt tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat
    57901 gattcgttta aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac
    57961 ccaaaataaa gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca
    58021 actattaatt caagatattc ttttttggtt agtttttcat tgctattatc catagttacc
    58081 tcctattttg tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg
    58141 ttttattatt gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac
    58201 tttactatta atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact
    58261 aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc
    58321 cttcgcaaaa attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc
    58381 atcgtactct ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc
    58441 ttcaaaagta tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc
    58501 tggagtaata gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa
    58561 aaattcttca aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc
    58621 agatgatgag ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa
    58681 acctacggta gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt
    58741 ttttttgata aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt
    58801 aaaaccaaat ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa
    58861 accaagcaat tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa
    58921 caactcttta tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt
    58981 atctaatata gattgttgct tctctatata tttttttaaa ttttccattt cattccaata
    59041 tttttttgta aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact
    59101 ttcgtttccg cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa
    59161 ttgtacggct gatatatttt taaaggggat agttttttct cctttaatac cttgattaac
    59221 taaatttata aatccttttc taataattga aatagaatca tcagttactt taatcaaggt
    59281 ttttcctgga gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata
    59341 tgattttttt tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt
    59401 ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct
    59461 tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag
    59521 cttattctga atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt
    59581 cagttcgttc tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt
    59641 ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga
    59701 ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag
    59761 attttcattc aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc
    59821 catataaact actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca
    59881 aaccatcact ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc
    59941 attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa
    60001 tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg
    60061 aaatagtcag caatccgact taattcatct gaattaggag tattattttt ccactttgct
    60121 agataaccgt tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc
    60181 ttcgccaagt cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt
    60241 tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta
    60301 tactatatct tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg
    60361 taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc
    60421 tcttagtata gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt
    60481 tgtttacgaa tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc
    60541 gaacaaaacg aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac
    60601 gttgcgagtg ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg
    60661 gaagataaga tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc
    60721 aacgaatctg gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt
    60781 aaacgttggg taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact
    60841 ccagaaaaag tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa
    60901 ctaaaagaag aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct
    60961 aaaatctcct atttagatag catattatct tctacagatt cagtaacaat tagtcagatt
    61021 gcagcagatt atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt
    61081 cagaaaaaag tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca
    61141 aaatctcata caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc
    61201 aaatggacac agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac
    61261 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    61321 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa
    61381 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    61441 acggtaaaaa gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg
    61501 aattgtaaag ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag
    61561 gctatgtaat tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta
    61621 gatggatgac gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt
    61681 ctagagtttt aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg
    61741 gatttgtcta ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac
    61801 ttgaattttt agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt
    61861 aatagagcgc tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    61921 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
    61981 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
    62041 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
    62101 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
    62161 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
    62221 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
    62281 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
    62341 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
    62401 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
    62461 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
    62521 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
    62581 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
    62641 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
    62701 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
    62761 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
    62821 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
    62881 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
    62941 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
    63001 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    63061 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
    63121 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    63181 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    63241 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
    63301 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
    63361 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
    63421 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
    63481 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
    63541 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
    63601 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
    63661 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
    63721 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
    63781 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
    63841 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    63901 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    63961 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
    64021 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
    64081 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt
    64141 ttagattatc gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg
    64201 aagcgatgct ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg
    64261 gaagatattc caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt
    64321 ctagaagctt tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt
    64381 aatgagcaga cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta
    64441 agctatgagc aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat
    64501 aagaagtgat atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt
    64561 tcaaaggaaa agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga
    64621 cgatgtatga cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac
    64681 gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata
    64741 caggcatgcc aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag
    64801 ggcgcaaaat aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag
    64861 caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga
    64921 ttttacctgc gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca
    64981 tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc
    65041 gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag
    65101 agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat
    65161 taaaccagaa aacaatcaaa aagttaaata ttggagggga aaaatgacag aacatcgaag
    65221 ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa
    65281 gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa
    65341 tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga
    65401 tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat
    65461 aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat
    65521 tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga
    65581 atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata
    65641 cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt
    65701 ccctaatgat taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa
    65761 gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc
    65821 taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc
    65881 aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa
    65941 agcaccattc taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata
    66001 gcgaattata aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa
    66061 gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaaact cagatactat
    66121 ttcgcactct atatcgatgg aacgcaatta agaaatttag catataccaa attactattc
    66181 attgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga
    66241 tggacctaaa gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa
    66301 gtgtgtcgta cgaagacaag tggctcaaca catttcatgg cggacgtgag agcgcacttg
    66361 atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag
    66421 cgaattatcg aattgactta ttcagaagat tggcaggaag ataaaaaaat agttgcagaa
    66481 gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag
    66541 attgcaatct ggcatggtga tcgaattcta gtaacgggta ccgctaaaca gttatctgaa
    66601 attactggtc tgagcaaaaa tattatttgg gatagagcta ggagcttatg gattgattca
    66661 aaaggaagac agtttaggta tgtggaggag aaataatgga cgaactaatc gcgaaagtag
    66721 agcagtgggc aaaagataag ggattggatc aagcggattt cagcaagcaa atgttaaaaa
    66781 cggtcgaaga aatcggggaa gttgccgctt ctctagctag aaaagatgaa catggtttaa
    66841 gagacggaat tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg
    66901 atttatacga gtgcttggat tttgcttatg acgaaatcaa aggtcggact ggaaagatgg
    66961 tcaatggtgt tttcgttaag tccagtgacc tgaaataatt ccattaatgg aagaaataac
    67021 tgaataggag gatagacatg attgatgcaa aacaagtgca aaaacagaaa gatggaatgt
    67081 tgatgtttga agcatatgtt ctaccatttc ttaatcaatt tgaagttttg gaatgctctg
    67141 ccagcggaga agaattggag tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc
    67201 taaacgaatt tctttgcact attaactgtt gggatatgat agctccagga tttttatgtc
    67261 cagctatggg agaatttttg gaatattgtc gtcttgaaga tgcaggcact ttggatttag
    67321 cttatctggt atacaactac ctaaacatca acacagatca tttgtggttt ggcactgccg
    67381 aaagaaaatg ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag
    67441 tggaattgcc gccattgctg taaggttctt tacgcgactc aaggtcacag aaatataaat
    67501 gcaaatctct tttgaagtga tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga
    67561 ataaatgaga tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc caaagaaccg
    67621 aatgagctaa ggacatcatg tgtaaacaca taatatagct ctcctttcac ggctaatcat
    67681 aacatgaaaa atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat
    67741 tatcccggca gtaacagatt ctgcaattaa actccgaagc tgatgtatca gtatcaactg
    67801 tagaatatta tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca
    67861 acggtcttat ctgtttagat gaaggcgata gtatgaatga aattgctgca gaccatgaat
    67921 tatcagaaac tgctttaatt gatcaaatcg aattagctag acaatacttt gttccatatg
    67981 cagatacctg gtccaagcat aagaatgaca tcatttttcc agaggaacaa tgagagcaac
    68041 tgatccagta attatccttg aggaagccaa atttatttgg actcacgaag agatagagca
    68101 agcacgcttg ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa taatgggtca
    68161 aaagattctt gatgtcggat tgcttttgct ccatctagca gaaaaaattt gatttgaggt
    68221 gaaaatgatg attcaacttg caggcatcca aacagggaaa atttattttt ctggagaaag
    68281 caaaagcgaa gccaaccaat ggctattgaa aacttacacg aacaataaga agctaaggaa
    68341 aaaatatcct gatttgttgc taaaggatga tcagattatg ccggaaccga tgattttgac
    68401 ccgtaaggag ggaagttgaa actaaaaaac cagccgtgtg ggctggagtt gctatttctt
    68461 tttaataatg agtttttcta aatttaattc attctcacga gtttgcgtcc aataatcaga
    68521 ataatatttt aattgtttaa gagtaggaat gtcgtcttcg gtaaaattag aa
//
LOCUS       DAYYCM010000007        65484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000007 DAYYCM010000000
VERSION     DAYYCM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.007"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHAY1_000508"
     CDS             476..1339
                     /locus_tag="ACHAY1_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8592533.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHAY1_000509"
     CDS             complement(1468..3090)
                     /locus_tag="ACHAY1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8592534.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHAY1_000510"
     CDS             3339..3860
                     /locus_tag="ACHAY1_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8592535.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAY1_000511"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAY1_000511"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8592536.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHAY1_000512"
     CDS             5941..6759
                     /locus_tag="ACHAY1_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8592537.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHAY1_000513"
     CDS             complement(6862..7059)
                     /locus_tag="ACHAY1_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592538.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHAY1_000514"
     CDS             7228..7806
                     /locus_tag="ACHAY1_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8592539.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHAY1_000515"
     CDS             7901..8038
                     /locus_tag="ACHAY1_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592540.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHAY1_000516"
     CDS             complement(8851..9354)
                     /locus_tag="ACHAY1_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8592541.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHAY1_000517"
     CDS             9713..10738
                     /locus_tag="ACHAY1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8592542.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACHAY1_000518"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="ACHAY1_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12415..12531
                     /locus_tag="ACHAY1_000519"
     CDS             12415..12531
                     /locus_tag="ACHAY1_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8592543.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACHAY1_000520"
     CDS             13459..13797
                     /locus_tag="ACHAY1_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592544.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACHAY1_000521"
     CDS             13891..14121
                     /locus_tag="ACHAY1_000521"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8592545.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACHAY1_000522"
     CDS             14521..14892
                     /locus_tag="ACHAY1_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8592546.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACHAY1_000523"
     CDS             complement(15166..15858)
                     /locus_tag="ACHAY1_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8592547.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACHAY1_000524"
     CDS             complement(15861..16646)
                     /locus_tag="ACHAY1_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8592548.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACHAY1_000525"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACHAY1_000525"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8592549.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACHAY1_000526"
     CDS             18308..18625
                     /locus_tag="ACHAY1_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8592550.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACHAY1_000527"
     CDS             18638..20311
                     /locus_tag="ACHAY1_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8592551.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACHAY1_000528"
     CDS             20325..21956
                     /locus_tag="ACHAY1_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8592552.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHAY1_000529"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHAY1_000529"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8592553.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHAY1_000530"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHAY1_000530"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8592554.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACHAY1_000531"
     CDS             25190..25795
                     /locus_tag="ACHAY1_000531"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8592555.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACHAY1_000532"
     CDS             26103..27119
                     /locus_tag="ACHAY1_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8592556.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACHAY1_000533"
     CDS             27260..28561
                     /locus_tag="ACHAY1_000533"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8592557.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACHAY1_000534"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACHAY1_000534"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8592558.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACHAY1_000535"
     CDS             29290..29688
                     /locus_tag="ACHAY1_000535"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8592559.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACHAY1_000536"
     CDS             29754..30836
                     /locus_tag="ACHAY1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8592560.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACHAY1_000537"
     CDS             31048..32610
                     /locus_tag="ACHAY1_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592561.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACHAY1_000538"
     CDS             32603..33736
                     /locus_tag="ACHAY1_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592562.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACHAY1_000539"
     CDS             33736..34692
                     /locus_tag="ACHAY1_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592563.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACHAY1_000540"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACHAY1_000540"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8592564.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACHAY1_000541"
     CDS             35981..36799
                     /locus_tag="ACHAY1_000541"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8592565.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACHAY1_000542"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACHAY1_000542"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8592566.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACHAY1_000543"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACHAY1_000543"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8592567.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="ACHAY1_000544"
     CDS             complement(38679..39260)
                     /locus_tag="ACHAY1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8592568.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACHAY1_000545"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACHAY1_000545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8592569.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="ACHAY1_000546"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="ACHAY1_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8592570.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="ACHAY1_000547"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="ACHAY1_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8592571.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="ACHAY1_000548"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="ACHAY1_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8592572.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="ACHAY1_000549"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="ACHAY1_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8592573.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACHAY1_000550"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACHAY1_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8592574.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="ACHAY1_000551"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="ACHAY1_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8592575.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="ACHAY1_000552"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="ACHAY1_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8592576.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="ACHAY1_000553"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="ACHAY1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8592577.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="ACHAY1_000554"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="ACHAY1_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8592578.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="ACHAY1_000555"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="ACHAY1_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8592579.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="ACHAY1_000556"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="ACHAY1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8592580.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="ACHAY1_000557"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="ACHAY1_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8592581.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="ACHAY1_000558"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="ACHAY1_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8592582.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="ACHAY1_000559"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="ACHAY1_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8592583.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACHAY1_000560"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACHAY1_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8592584.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="ACHAY1_000561"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="ACHAY1_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8592585.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="ACHAY1_000562"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="ACHAY1_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8592586.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="ACHAY1_000563"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="ACHAY1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8592587.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="ACHAY1_000564"
     CDS             51714..51899
                     /locus_tag="ACHAY1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8592588.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="ACHAY1_000565"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="ACHAY1_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8592589.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="ACHAY1_000566"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="ACHAY1_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8592590.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="ACHAY1_000567"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="ACHAY1_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8592591.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="ACHAY1_000568"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="ACHAY1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8592592.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="ACHAY1_000569"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="ACHAY1_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8592593.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="ACHAY1_000570"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="ACHAY1_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8592594.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="ACHAY1_000571"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="ACHAY1_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8592595.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="ACHAY1_000572"
     CDS             55974..56621
                     /locus_tag="ACHAY1_000572"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8592596.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="ACHAY1_000573"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="ACHAY1_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8592597.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACHAY1_000574"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACHAY1_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8592598.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="ACHAY1_000575"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="ACHAY1_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8592599.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="ACHAY1_000576"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="ACHAY1_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8592600.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="ACHAY1_000577"
     CDS             58059..58997
                     /locus_tag="ACHAY1_000577"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8592601.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="ACHAY1_000578"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="ACHAY1_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8592602.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="ACHAY1_000579"
     CDS             59677..60516
                     /locus_tag="ACHAY1_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8592603.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="ACHAY1_000580"
     CDS             60492..61361
                     /locus_tag="ACHAY1_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8592604.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="ACHAY1_000581"
     CDS             61351..62148
                     /locus_tag="ACHAY1_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8592605.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62351..63097
                     /gene="truA"
                     /locus_tag="ACHAY1_000582"
     CDS             62351..63097
                     /gene="truA"
                     /locus_tag="ACHAY1_000582"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8592606.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63761..63955
                     /locus_tag="ACHAY1_000583"
                     /pseudo
     CDS             63761..63955
                     /locus_tag="ACHAY1_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(64143..64919)
                     /locus_tag="ACHAY1_000584"
     CDS             complement(64143..64919)
                     /locus_tag="ACHAY1_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8592607.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            65042..65449
                     /locus_tag="ACHAY1_000585"
     CDS             65042..65449
                     /locus_tag="ACHAY1_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8592608.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        20795 a        11677 c        13821 g        19191 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg gtattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtagggt
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg tagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttga gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcgagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaaattttat aaaaaaatca ttattccaga taacctttta ggaacaaagg
    62341 agagaaaaac atggtacgtt acaaagcaat cattgcatac gacggaacaa attttaatgg
    62401 attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa
    62461 aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg ccggagtaca
    62521 tgcgattggg caagtaatcc attttgatta tccagaggag cgtccgttag aacgaatgcg
    62581 tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag aaattgtttc
    62641 agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc gagtggatat
    62701 agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt accctcttga
    62761 tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt tcacctcttt
    62821 ttgtgcatca ggaagctcga tagaggataa agtacgtacg atctacgagg ctaagatgga
    62881 ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct tcttgtataa
    62941 gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat tgcctgaaga
    63001 tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta cagcacatcc
    63061 tgaaggactt tatttgtatg aagtgaaata tgaataagag gttgtgacag aagtggtcag
    63121 cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aaattttgca aaatttcggc
    63181 ttattttcga aggagctact tctggaacac cgtgaatcag aggttgtgaa aaagctgccc
    63241 tgcatcaagt aataagaaca accaaacaaa aatagctcgt catatttttc gtttggttgg
    63301 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    63361 gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
    63421 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
    63481 tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc tttgatgaat
    63541 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
    63601 gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag tcggaacagg
    63661 tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat ttagcgctga
    63721 aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag aatatttacg
    63781 ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac gcgtttttgc
    63841 agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg aaaacaatca
    63901 ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca cttggcagtt
    63961 ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca gtctacactc
    64021 ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa ctaagtactt
    64081 tattttactc tttaggattg atacagtagc atctatccct ttattaataa tcttttattt
    64141 ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga aattcctttt
    64201 tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac agcttccata
    64261 tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa atcgtctttc
    64321 cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc tactatgatt
    64381 tctggattat actgatcaat agtttgttta acagcttcat accaaactgt atctcctgta
    64441 aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt acctacataa
    64501 ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat agattcaaaa
    64561 actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat ttcattcgca
    64621 tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt cgctatatcg
    64681 tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt gataatatct
    64741 tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg tgatggaaaa
    64801 ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg atcaattaaa
    64861 aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg tatattcata
    64921 aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt ccatacaata
    64981 aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa ggttgtttac
    65041 catgttagac caaacagata taaatatcat tgaggaacta tcaaaaaata gtcgaatcac
    65101 aatgaaagaa cttggagaaa aagtacattt gactggaccc gctgtctcgg cgagagtgac
    65161 aaaactagag gaaagtggag ttattgaaaa ttatacagta aaattaaata tggaaaagct
    65221 agggttttcg attcacgctt ttttaattat aattacgcaa aatttaaatc ataaacccta
    65281 tttatcattt attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg
    65341 gggctgttat cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga
    65401 acagcttaat gaatatgcga attataaatt atctattgtt atagattaat aataaaacta
    65461 gcagatggta aaaatactaa tatg
//
LOCUS       DAYYCM010000008        59719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000008 DAYYCM010000000
VERSION     DAYYCM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.008"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..802)
                     /locus_tag="ACHAY1_000586"
     CDS             complement(<1..802)
                     /locus_tag="ACHAY1_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8592609.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKV"
     gene            complement(1026..2966)
                     /gene="thrS"
                     /locus_tag="ACHAY1_000587"
     CDS             complement(1026..2966)
                     /gene="thrS"
                     /locus_tag="ACHAY1_000587"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8592610.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3303..3482)
                     /locus_tag="ACHAY1_000588"
     CDS             complement(3303..3482)
                     /locus_tag="ACHAY1_000588"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8592611.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3529..5352)
                     /locus_tag="ACHAY1_000589"
     CDS             complement(3529..5352)
                     /locus_tag="ACHAY1_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8592612.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5630..7909
                     /gene="helD"
                     /locus_tag="ACHAY1_000590"
     CDS             5630..7909
                     /gene="helD"
                     /locus_tag="ACHAY1_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8592613.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8002..8649)
                     /locus_tag="ACHAY1_000591"
     CDS             complement(8002..8649)
                     /locus_tag="ACHAY1_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592614.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8828..9547)
                     /locus_tag="ACHAY1_000592"
     CDS             complement(8828..9547)
                     /locus_tag="ACHAY1_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8592615.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9678..10829)
                     /locus_tag="ACHAY1_000593"
     CDS             complement(9678..10829)
                     /locus_tag="ACHAY1_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8592616.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11046..11786)
                     /locus_tag="ACHAY1_000594"
     CDS             complement(11046..11786)
                     /locus_tag="ACHAY1_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8592617.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11790..12113)
                     /gene="rsfS"
                     /locus_tag="ACHAY1_000595"
     CDS             complement(11790..12113)
                     /gene="rsfS"
                     /locus_tag="ACHAY1_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8592618.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12170..12763)
                     /gene="yqeK"
                     /locus_tag="ACHAY1_000596"
     CDS             complement(12170..12763)
                     /gene="yqeK"
                     /locus_tag="ACHAY1_000596"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8592619.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12747..13397)
                     /locus_tag="ACHAY1_000597"
     CDS             complement(12747..13397)
                     /locus_tag="ACHAY1_000597"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8592620.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13407..13718)
                     /gene="yhbY"
                     /locus_tag="ACHAY1_000598"
     CDS             complement(13407..13718)
                     /gene="yhbY"
                     /locus_tag="ACHAY1_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8592621.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13734..14843)
                     /gene="yqeH"
                     /locus_tag="ACHAY1_000599"
     CDS             complement(13734..14843)
                     /gene="yqeH"
                     /locus_tag="ACHAY1_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8592622.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14840..15367)
                     /locus_tag="ACHAY1_000600"
     CDS             complement(14840..15367)
                     /locus_tag="ACHAY1_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8592623.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15547..16332)
                     /locus_tag="ACHAY1_000601"
     CDS             complement(15547..16332)
                     /locus_tag="ACHAY1_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8592624.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16325..17194)
                     /gene="accD"
                     /locus_tag="ACHAY1_000602"
     CDS             complement(16325..17194)
                     /gene="accD"
                     /locus_tag="ACHAY1_000602"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8592625.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17195..18574)
                     /locus_tag="ACHAY1_000603"
     CDS             complement(17195..18574)
                     /locus_tag="ACHAY1_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8592626.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18582..19001)
                     /gene="fabZ"
                     /locus_tag="ACHAY1_000604"
     CDS             complement(18582..19001)
                     /gene="fabZ"
                     /locus_tag="ACHAY1_000604"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8592627.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19026..19502)
                     /gene="accB"
                     /locus_tag="ACHAY1_000605"
     CDS             complement(19026..19502)
                     /gene="accB"
                     /locus_tag="ACHAY1_000605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8592628.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19505..20740)
                     /gene="fabF"
                     /locus_tag="ACHAY1_000606"
     CDS             complement(19505..20740)
                     /gene="fabF"
                     /locus_tag="ACHAY1_000606"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8592629.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20759..21496)
                     /gene="fabG"
                     /locus_tag="ACHAY1_000607"
     CDS             complement(20759..21496)
                     /gene="fabG"
                     /locus_tag="ACHAY1_000607"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8592630.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21508..22425)
                     /gene="fabD"
                     /locus_tag="ACHAY1_000608"
     CDS             complement(21508..22425)
                     /gene="fabD"
                     /locus_tag="ACHAY1_000608"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8592631.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22532..22756)
                     /locus_tag="ACHAY1_000609"
     CDS             complement(22532..22756)
                     /locus_tag="ACHAY1_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8592632.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIEEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22809..23771)
                     /locus_tag="ACHAY1_000610"
     CDS             complement(22809..23771)
                     /locus_tag="ACHAY1_000610"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8592633.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23781..24221)
                     /locus_tag="ACHAY1_000611"
     CDS             complement(23781..24221)
                     /locus_tag="ACHAY1_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8592634.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24527..25492
                     /locus_tag="ACHAY1_000612"
     CDS             24527..25492
                     /locus_tag="ACHAY1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8592635.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25549..25779)
                     /locus_tag="ACHAY1_000613"
     CDS             complement(25549..25779)
                     /locus_tag="ACHAY1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8592636.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25865..26752)
                     /locus_tag="ACHAY1_000614"
     CDS             complement(25865..26752)
                     /locus_tag="ACHAY1_000614"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8592637.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26841..28064)
                     /locus_tag="ACHAY1_000615"
     CDS             complement(26841..28064)
                     /locus_tag="ACHAY1_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8592638.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28081..29127)
                     /locus_tag="ACHAY1_000616"
     CDS             complement(28081..29127)
                     /locus_tag="ACHAY1_000616"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8592639.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29291..31018)
                     /gene="ptsP"
                     /locus_tag="ACHAY1_000617"
     CDS             complement(29291..31018)
                     /gene="ptsP"
                     /locus_tag="ACHAY1_000617"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8592640.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31018..31284)
                     /locus_tag="ACHAY1_000618"
     CDS             complement(31018..31284)
                     /locus_tag="ACHAY1_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8592641.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31763..33997
                     /locus_tag="ACHAY1_000619"
     CDS             31763..33997
                     /locus_tag="ACHAY1_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8592642.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34145..34465
                     /locus_tag="ACHAY1_000620"
     CDS             34145..34465
                     /locus_tag="ACHAY1_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8592643.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34670..34894)
                     /locus_tag="ACHAY1_000621"
     CDS             complement(34670..34894)
                     /locus_tag="ACHAY1_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8592644.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35092..36672)
                     /locus_tag="ACHAY1_000622"
     CDS             complement(35092..36672)
                     /locus_tag="ACHAY1_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8592645.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37073..38449)
                     /locus_tag="ACHAY1_000623"
     CDS             complement(37073..38449)
                     /locus_tag="ACHAY1_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8592646.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38577..39665)
                     /locus_tag="ACHAY1_000624"
     CDS             complement(38577..39665)
                     /locus_tag="ACHAY1_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8592647.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39837..40271
                     /locus_tag="ACHAY1_000625"
     CDS             39837..40271
                     /locus_tag="ACHAY1_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592648.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40349..40882)
                     /locus_tag="ACHAY1_000626"
     CDS             complement(40349..40882)
                     /locus_tag="ACHAY1_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8592649.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40976..42154)
                     /gene="mutY"
                     /locus_tag="ACHAY1_000627"
     CDS             complement(40976..42154)
                     /gene="mutY"
                     /locus_tag="ACHAY1_000627"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8592650.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42147..42944)
                     /gene="recX"
                     /locus_tag="ACHAY1_000628"
     CDS             complement(42147..42944)
                     /gene="recX"
                     /locus_tag="ACHAY1_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8592651.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43051..44433
                     /gene="rlmD"
                     /locus_tag="ACHAY1_000629"
     CDS             43051..44433
                     /gene="rlmD"
                     /locus_tag="ACHAY1_000629"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8592652.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44607..45185)
                     /locus_tag="ACHAY1_000630"
     CDS             complement(44607..45185)
                     /locus_tag="ACHAY1_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8592653.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45507..45977
                     /locus_tag="ACHAY1_000631"
     CDS             45507..45977
                     /locus_tag="ACHAY1_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8592654.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46085..47311)
                     /locus_tag="ACHAY1_000632"
     CDS             complement(46085..47311)
                     /locus_tag="ACHAY1_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8592655.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48065..48265)
                     /locus_tag="ACHAY1_000633"
     CDS             complement(48065..48265)
                     /locus_tag="ACHAY1_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8592656.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48399..49007)
                     /locus_tag="ACHAY1_000634"
     CDS             complement(48399..49007)
                     /locus_tag="ACHAY1_000634"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8592657.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49092..49562)
                     /locus_tag="ACHAY1_000635"
     CDS             complement(49092..49562)
                     /locus_tag="ACHAY1_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8592658.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49610..50524)
                     /locus_tag="ACHAY1_000636"
     CDS             complement(49610..50524)
                     /locus_tag="ACHAY1_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8592659.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50670..51473
                     /locus_tag="ACHAY1_000637"
     CDS             50670..51473
                     /locus_tag="ACHAY1_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8592660.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51651..52598
                     /locus_tag="ACHAY1_000638"
     CDS             51651..52598
                     /locus_tag="ACHAY1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8592661.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52666..52959)
                     /locus_tag="ACHAY1_000639"
     CDS             complement(52666..52959)
                     /locus_tag="ACHAY1_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8592662.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(52987..53652)
                     /locus_tag="ACHAY1_000640"
     CDS             complement(52987..53652)
                     /locus_tag="ACHAY1_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8592663.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53792..54424)
                     /locus_tag="ACHAY1_000641"
     CDS             complement(53792..54424)
                     /locus_tag="ACHAY1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8592664.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54431..55498)
                     /gene="liaS"
                     /locus_tag="ACHAY1_000642"
     CDS             complement(54431..55498)
                     /gene="liaS"
                     /locus_tag="ACHAY1_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8592665.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55495..56226)
                     /gene="liaF"
                     /locus_tag="ACHAY1_000643"
     CDS             complement(55495..56226)
                     /gene="liaF"
                     /locus_tag="ACHAY1_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8592666.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56395..56874)
                     /gene="greA"
                     /locus_tag="ACHAY1_000644"
     CDS             complement(56395..56874)
                     /gene="greA"
                     /locus_tag="ACHAY1_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8592667.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57010..58184)
                     /gene="mltG"
                     /locus_tag="ACHAY1_000645"
                     /pseudo
     CDS             complement(57010..58184)
                     /gene="mltG"
                     /locus_tag="ACHAY1_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            58388..59557
                     /locus_tag="ACHAY1_000646"
     CDS             58388..59557
                     /locus_tag="ACHAY1_000646"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8592668.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        17317 a        12560 c        10087 g        19755 t
ORIGIN      
        1 tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg
       61 tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc
      121 tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca
      181 acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac
      241 ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg
      301 ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt
      361 gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg
      421 ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt
      481 cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat
      541 ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga
      601 ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg
      661 cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat
      721 taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt
      781 caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta
      841 taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc
      901 ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag
      961 gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta caggaaattt
     1021 acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata
     1081 ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct
     1141 tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt
     1201 atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc
     1261 aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt
     1321 gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa
     1381 gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta
     1441 ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat
     1501 tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt
     1561 ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata
     1621 gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct
     1681 gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca
     1741 tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga
     1801 acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca
     1861 cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg taattcacga
     1921 taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga
     1981 acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc
     2041 cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg
     2101 taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc
     2161 ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca
     2221 agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat
     2281 tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg
     2341 tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg
     2401 ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca
     2461 gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca
     2521 aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg
     2581 acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt
     2641 ccattatctg tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata
     2701 tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag
     2761 ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga
     2821 cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt
     2881 tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca
     2941 ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca
     3001 gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga
     3061 aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt
     3121 ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga
     3181 aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc
     3241 atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa
     3301 acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg
     3361 acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc
     3421 atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc
     3481 ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt
     3541 tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa
     3601 aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc
     3661 cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt
     3721 cgctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc
     3781 tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc
     3841 aataccgata tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc
     3901 aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct
     3961 acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc
     4021 atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt
     4081 tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat
     4141 tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg
     4201 tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt
     4261 cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa
     4321 gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat
     4381 tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt
     4441 atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac
     4501 gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat
     4561 cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa
     4621 ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc
     4681 agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat
     4741 ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt
     4801 taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt
     4861 caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag
     4921 ttcgccaggc ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg
     4981 caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag
     5041 ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat
     5101 caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat
     5161 cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat
     5221 caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg
     5281 aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt
     5341 gaaatgtgtc attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt
     5401 ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc
     5461 ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa
     5521 atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac
     5581 tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa
     5641 acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat
     5701 gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc
     5761 taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg
     5821 ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct
     5881 gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga
     5941 ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac
     6001 aatcgttatt gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc
     6061 cgcattttat gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt
     6121 taaaatccaa gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga
     6181 ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac
     6241 gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga
     6301 aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta
     6361 tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt
     6421 ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac
     6481 attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca
     6541 agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac
     6601 cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg
     6661 tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa
     6721 agaaacaaat gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct
     6781 gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc
     6841 ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa
     6901 ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat
     6961 ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct
     7021 acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga
     7081 acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt
     7141 actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta
     7201 tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt
     7261 gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga
     7321 aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt
     7381 aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa
     7441 agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac
     7501 aacagatgat atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc
     7561 ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc
     7621 tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat
     7681 gaagcgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt
     7741 tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta
     7801 caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc
     7861 tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca
     7921 actgctatca ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt
     7981 aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt
     8041 taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc
     8101 aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca
     8161 ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat
     8221 ctctgataca cgaaatgcta ggtatgcact atcgatcact ttattttttt tcgtgtctat
     8281 cttctcattt ctttcgataa gaattttttc aggacgatgt ttcaagctcc actctaatag
     8341 tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg
     8401 acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac
     8461 catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc
     8521 ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc
     8581 taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt
     8641 tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt
     8701 atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag
     8761 acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac
     8821 tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt
     8881 ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt
     8941 tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg
     9001 cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt
     9061 ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt
     9121 cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa
     9181 cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca
     9241 cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc
     9301 gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa
     9361 tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg
     9421 catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat
     9481 aaatccggct attattagca tctttagcag ttttctttgc tacgatattt gcccatttac
     9541 gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt
     9601 ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct
     9661 catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata
     9721 ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct
     9781 ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca
     9841 gcatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc
     9901 tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt
     9961 tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc
    10021 atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa
    10081 gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag cgaacaagtt
    10141 gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac
    10201 gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag
    10261 cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc
    10321 tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga
    10381 tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt
    10441 tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg
    10501 taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc
    10561 aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga
    10621 agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg
    10681 atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct
    10741 gctcctgtta tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg
    10801 tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa
    10861 agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact
    10921 attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat
    10981 cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa
    11041 aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg
    11101 tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa
    11161 taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca
    11221 gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt
    11281 cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct
    11341 ggaaaaacct catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct
    11401 tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag
    11461 cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc
    11521 atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc
    11581 atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca
    11641 accgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa
    11701 tgacgtttcg aaaaagctaa ccattgctga taaagtgttt cgtccatcac ttcatcgtaa
    11761 atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag atcgactagc
    11821 ggagcatctg accatagttt ttccagatta tagaaatttc gttctggcgc atggaaaaca
    11881 tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata
    11941 cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg
    12001 atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt
    12061 cgaacgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aatcgcaatt
    12121 tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg
    12181 ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca
    12241 aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa
    12301 tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat
    12361 aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat
    12421 gaagaatctc tgcatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac
    12481 catgccagat tgcattatta taattcaata actccggatc aaaaccgtct cttacgatta
    12541 ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac
    12601 tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc
    12661 caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt
    12721 ctctagaata agcagtatat tcattactat agtccatatt catacaaccc ttcattttga
    12781 atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt
    12841 tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat
    12901 ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc
    12961 tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa
    13021 taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc
    13081 ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc caccgctaat
    13141 tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc
    13201 tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc
    13261 atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt
    13321 ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta
    13381 gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga tagcctttga
    13441 tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt
    13501 tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt
    13561 tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc
    13621 attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg
    13681 ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc
    13741 aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc
    13801 gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa
    13861 aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat
    13921 aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata
    13981 tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat
    14041 cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg
    14101 atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga
    14161 tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg
    14221 attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct
    14281 gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata
    14341 tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga
    14401 gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt
    14461 tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg
    14521 ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa
    14581 ccattgaagt caaaaatatc caccacattg acgatcaatg catctttttg ccctaattca
    14641 ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat cttgggtctc attataatgg
    14701 cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc
    14761 gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg
    14821 atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg
    14881 ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat
    14941 ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta
    15001 tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt
    15061 tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat
    15121 caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag
    15181 ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat
    15241 tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct
    15301 ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg
    15361 aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc
    15421 cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa
    15481 agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa
    15541 aaacaactaa tattggcgga acctcttgta tcgtttttcc agtaatttat gctgatctaa
    15601 tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc ataatgtgtg cgactttata
    15661 tcgtggcaac tgctcgccgt tgaacgtttc aggaataact cgatcaacga ttcctaactc
    15721 gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct tctgcggcgc gactaccgtc
    15781 tttccacaaa atcgacgcaa atccttctgg agaaagcaca gcataaatcg tttcttccag
    15841 catccagact tcatccgcta aacctagagc aagggcaccg ccacttccac cttcaccaat
    15901 gatgatggcg atgatcggta cctttaaatc agacatttcg agcagatttc tggcaatagc
    15961 ctctccttcg ccgcgctctt ctgctcctac accgcaataa gcacctgctg tattgatcaa
    16021 ggtaatcacc ggtctattga acttttccgc ttgtttcatc agtcgtaaag cttttcgata
    16081 gccttcagga tggggagaac caaaatttct gcggatattt tcctgcagat cccgtccttt
    16141 ttgaataccg atcactgtta ccggtttgcc atctaatcga gcgattccac caacaatcgc
    16201 ttcgtcatct gcaaataaac gatcaccatg gcattcctcg aaagaatcga agatctcttc
    16261 aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct aactgaacga catcatgtgc
    16321 tgtcttatcc acgatctatc catcctttct gtgtatgcaa ttcaagaagc gtatggattt
    16381 tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa accatgagat aataagaatt
    16441 cagccttttg gaaatcttct ggaagttcct gcttgatcgt ctgttcgatg acgcgtcttc
    16501 cagcaaaacc gatcaagctt tggggttctg ctagaataat atcgccttcc attgcaaaac
    16561 tggcagttac tcctccggtc gtcgggtccg ttaaaacagt tagataaaaa agaccttcat
    16621 tgctatgccg tttgacagca gcagagattt ttgccatctg cattagagaa aagattcctt
    16681 cttgcatccg tgcgccacct gaagctgtga acaagacgac tggaagactt tcctttgttg
    16741 ctcgttcgaa taatctggtg atcttttctc cgacaatcgt tcccatactt cccatgataa
    16801 aattaggatc catcactcca ataatgaagg gaatcccctg aatggttgct ttaccagtga
    16861 ggactgcttc atgaagtcct gttttatctt gcattttttt aattttttcc gtatattcgg
    16921 gaaatgctag cggatctttg gttacaagat ccgtatccca ttcttcaaaa cttttttcat
    16981 caataatcaa agctagacgc tgccatgcac cgattcgaaa agcgtatccg caatgagggc
    17041 atactttttc ttcaccgatg tctttcgtat aaaggatatg tttgcaattt ggacactttg
    17101 cccacatgtt atcaggaaca gatggcgagt tagcaggttt gctctctcta ttaggattga
    17161 tccgaatata tttctttttc ttaaacagag ccatctatgc gcctcctatc tccgtttctg
    17221 gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt gctgtaatca cctgctatga
    17281 ctgcaggatg actaatcaaa tccatctgaa attctgcgtt cgtgatgatt ccttctgtga
    17341 taaattcact taaggctcgc tgcatcttca ttaatgcttc aaaacgcgta tttccatgga
    17401 cgattatttt tgcaatcatt gaatcataat atggcgggat cgtatatccc gggtaaactg
    17461 cactgtctac tcgcagtccc atcccacctg ctggaagcag taagttttgg atcttccctg
    17521 gagaaggagc aaaatggaaa gcaggatttt ctgcattaat ccggcattcg attgcgtgtc
    17581 cttggaatac tacttcttct tgacacacat tcaaaggctc accggctgct atctccacct
    17641 gtttttttac taaatcgatt cctgtgacca tttctgttac tggatgttcc acttggattc
    17701 gagtattcat ttccatgaaa taaaaatcgc cttcttcatc catcagaaac tcgatcgttc
    17761 cggcattttc ataattgact gctttagctg ctcgaacagc agcgtctcct agtgcttctc
    17821 tttttgtttg tgagatcact acagaaggag attcttccag tactttttga ttgttgcgtt
    17881 gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc aaatcgatca cctaagatct
    17941 gaacttctat atggcgagca ggataaatga ttttttccaa atacatatca tcatttccaa
    18001 atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg tgggagctct tcttttgatt
    18061 gaactttccg aatgcctttc ccgccaccac ctgcagctgc tttcagcatc actggataac
    18121 caattctgtc tgctatttcc aatgcttctt ctactgtaga taatacacca gtacttcctg
    18181 gaatgactgg gacatttgct tcttgcatca attgacgcgc attgatctta tttcccattg
    18241 catcgatcgt tgcactttta ggaccaataa atgtgatatt gcactcttca cacatggagg
    18301 caaatcggct attttcagat aaaaaaccaa atcctggatg aattgcttcg gcttttgtca
    18361 cgattgcagc gctcagtact tcctgaacat tcagatatga atcaacggct tttgccgggc
    18421 caatgcagat tgcttcatct gctaactgtg tatgaagtgc ttcggcatct gcttctgaat
    18481 atatcgctac ggtttggatc cctagttcgc ggcatgcacg aatgatacgt acagcgattt
    18541 caccgcggtt agctatcaat actttagaaa acatatttca cctatccaat cataaaggtt
    18601 aattccgctt cggctacttt tttaccgtct actttagcga ttcctttgcc tattccggct
    18661 gaagctttca ctttcaatat ctcaacttca aggtataagg tatctcccgg tgtaactttc
    18721 ttacggaatt ttgctttgtc caatccgccg aaataagccg tctttccctt gaattgttca
    18781 agagaaagta atgcaactgc tcctgcttga gccattgctt caacaatcaa tacccctggc
    18841 ataacaggtt cttctggaaa atgtccttga aaaaagggct cattgatcgt tacatttttc
    18901 ttagcaacga tccgttctcc ttcaaccatt tcttccactc gatcaatcaa aagcattgga
    18961 taacgatgtg gaatgatttc ttttatttct tgtatgttca ttgttttccc tctttcctgc
    19021 tttgcttatt tttgctgaat cctaaagagt ggctgtccat attcgacaac tgactcgttt
    19081 tctacaagga tttctgtgat cactccgtcg gcctcactag ttatctcatt catcagtttc
    19141 atcgcttcaa taatgcagat aacttctcct ttggcaactt gatcacctac ttttttgaaa
    19201 gctggtttgt cgggcgctgg ttgcagataa acgattccca cgattggcga aacaacctcg
    19261 attccatcaa atactgccga ttctttttct ttcattattt ctggagcagt tgttgcttgt
    19321 tcaggtatga cattgactgg ctccgaaact ggcaacgttt cgtttgtttg aaccatattc
    19381 atgcctcgac ttgttttatt tttattcata tacaattcaa attgaccttc acgcaaatcg
    19441 aattccgtta gcgaggattg atcaaattga ctgactaatt ctctgatttc attgatatcc
    19501 aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt atgtccgcca aaccctaatg
    19561 aattactcat tgcataagta aaacttgcag gtttgctttc gtttttgaca actgtcaagt
    19621 caatctcagg atcttgtttt tctacattga tcgtaggtgg gataaattga tgttctaatg
    19681 ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc caataaatgt ccagtcatcg
    19741 atttcgtgct gctgacgtaa gtatctctga aattttcacc taatgcgatt tggattgctt
    19801 ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg agcattgata tatccgactt
    19861 gttccggctg tacaccagct tcttccatcg caagcttcat tgctttagat gcaccagaac
    19921 catctggagt aggagctgtc atatggtaag cgtcacagtt tgcaccgtat ccaacgattt
    19981 ctccatagat tttggcaccg cgagcttgtg catgttctaa agattcaaga actagaacgc
    20041 ctgctccttc acccatcaca aaaccattcc gattttcgtc aaacggaatc gaaccacgat
    20101 taggatcagt tgattctgag agtgctgtta aagctgcaaa tccagcaatc cccatctcac
    20161 aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga atagccatgt ttgatgttac
    20221 ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc tgttacgata gctgtacaaa
    20281 taccttttgc acctacacgc aaagcaatat ttccagctgc catgttggca atcgccattg
    20341 gtacgaacat tggagaaact cgttttgcac ctttttcatg tagacgaata acttggtttt
    20401 cgattgttgg aagaccaccg atcccagatc cgatgatgac gccaaaccgg tcttgattca
    20461 ttttttcttt atctaaaccg ctcatctcca ttgcttctaa agcagcgtag attccataga
    20521 tcgaaaacat atccattcgt ttgctatctt ttttaataaa atatttatcg taagggaaat
    20581 ctttgacttc acccgctaag gtaatccctg tttctgaagc atcaaattta gtgatcggtg
    20641 cgatgccatt cgtcccctct tttaagctat tcaaaaagct gtctgcgtca tttccaatag
    20701 gtgaagtgac tccatagcca gtaattacca ctcgattcat ttgtttgctc tccttttctt
    20761 atccgtgcat gaccaatccg ccatcgacat tgatgacttg tccggtgata tatggatttt
    20821 gtgctaagaa aactgctgtt tgtgcaatat cttctacttg tccgaaacgc tgcatcggaa
    20881 tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac ttccgtcata tcagttgtga
    20941 tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt tgcagcttct cgtgccacag
    21001 attttgttaa tcctactacg ccagctttac tagctgcata atttgcttga ccaatatttc
    21061 caatcaaccc agatacacta gagagattaa tgatggcccc ttctcgctgc ttcatcattt
    21121 ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa attgatatcc agacattttt
    21181 tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt tgtgattccg gcattgttta
    21241 ctaacacatg gatcgatcca agcttttctt ctgcttcttt gatcatttgt cccgctttct
    21301 cataatcaga gatgtcaccg gaaactccta cgcattttac accgaatgct tcgatttctt
    21361 ctatcttttc ttttggaatc tctccgcgac cattcaaaat gatatttgct ccagcttttg
    21421 caaaagctaa ggccatcgct ttgccgattc ctcttgtact acctgtgata aatacatttt
    21481 ttccttttac atccataacg ttcctcctta tagtattgct attgtttcat caaatgtctg
    21541 cttattctca acacgcagaa ctggtattgt tttatcgatt tttttcataa atccgcttaa
    21601 tacttttccg gggccgactt cgatgacttg ttcgatgccg attgttttta acttatggat
    21661 actctcatag aaacgaacag gttgcatgac ttgctgttca agtagtcccg cgattgtttc
    21721 tttcttcatt atttccgttg ttgtgttgct tatgatagga aaagaaggtt cagagaattg
    21781 aatctgactt aggtccttcg ccagtttttt tgctgccggt tctaaaatag ctgtatgaaa
    21841 aggcccgctg acatttagag ggatcattct tttgaaccct ttttctttta atagtaaaac
    21901 agcttcgtct actgcttttt cttcgccacc aatcacgatc tgctgaggtg tattataatt
    21961 tgcaggagat acgatgccgt acttgctagc ttcatgacag ctttcctcga tcgtttcaat
    22021 cggcgcattc atcactgcaa ccattttccc gcttccagct ggggcagctt ctgtcatata
    22081 agcgcctctt tttgctacta acgcaactgc ttcagtgaag gatagcgcat tactagctac
    22141 taatgccgaa tattctccta aactcagtcc taaagctgca tctgggatga ttccatgctc
    22201 cttcaataag cgataatacg cgatgctgac tgttagaata gctggctgtg tatattgcgt
    22261 ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc atatcgtagc cgagaatctc
    22321 acttgcttca tcaaatgtct gctttacaac ggcttcttca tataaatctt ttcccatacc
    22381 ttgatattgt gcaccttggc cactaaataa gaatgctgtt ttcatgtttt tctccaatat
    22441 atccagaata tatgtttgat tcttcctatt caaaaaggcc gagacattcc tatttcagga
    22501 aatgtcccag cccccaacct aattttaatg attatgcagt ttgtttttct acgtaagtca
    22561 ctaaatcttg aaccgttttg attccgtctt ctgtttcgat tttcacgtca aattcatctt
    22621 caatttcatt gatgatttgg aataaatcca agctgtcagc ttctaagtct tcttcgatgt
    22681 cagtagtcaa tttgatttct tcagtttctt ttcctaattc ttctgcgatg atttcttgaa
    22741 ttttttcgaa taccataata atatattcct ccgatattgt ttgcttttta tttttataat
    22801 ttaacagtct acaactccat gagaatactt ccccatgtta aacctccgcc atagcctgta
    22861 aatattactt tctgctgttt tcctaaggtg atctttccac tagaaactgc ttcatccaat
    22921 aagataggga tgcttgcagc agatgtattc ccatatttat ccatattggt caggaacttt
    22981 tcttgcggca tctttacttt tcgggcaatt ttctcaataa ttctgaggtt tgcctgatgc
    23041 aaaagcagat aatcaactgg tttgtctttc atgacttctt ggatattgtt tgcaacgtct
    23101 cgtacggcaa aatcaaaaat atctctgcca gtcatttgca aataatacga accttctgaa
    23161 tcttctgaat agaacggatt tttattttgc tggtagccag cggtcaatga gctacttctg
    23221 gtaccgtcag ctgctaattt ttctttcagc aattttttct gtggattcgc ttctaagaga
    23281 atcccacctg ctccatcgcc aaacaacaca gcagtcgtac gatcagacca atcgattagc
    23341 ttagatagtg tttcaccacc aatcaccagt cccttagttg agccgcagcg gatcattttt
    23401 tcagccatgc ttaacgcata aacaaatcct gagcacgcag cactgatatc aaaagcgata
    23461 gcatgagttg ctcccaattt cccttgtact tgtgctgcta cagatggtgt cgtgtaatcg
    23521 ggcgacattg ttgctactaa aataaaatcc aaatctttag ggtttagatt tttctgtttt
    23581 atcaattttt ccgccactaa aatgcataaa tcagaagtct cctgatttgt tacacatctt
    23641 cgttcacgaa tacccgtcct tgacgaaatc cattcatcag aggtatccat catcttggaa
    23701 agatcattat tagtaataat cttttcagga aggacgctgg ctgttgcagt aatcattcca
    23761 aattgttcca ttaaatatcc tcacttatag tcttgcaaga agtcgtgcaa gtttttcagt
    23821 gcatccagca aggcatgctg ttcctccgta gccattccgt ctaagatgtt cttgaccatt
    23881 tcacgatgaa aatgctgatg gacccgaaat agtaactttc cttttttcgt tagtcccaac
    23941 tttacaacgc ggcggtcatc ttcactgcgg atacgttcga catagccttt ttttactaac
    24001 ttgttaatag cagtggtcaa ggtcccaaca gtaattgata gttctttggc tacttctgaa
    24061 gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt gcatctctgt gattgacaga
    24121 tctttgaatt gagatttttt cagctcagac tcttcgattg tcaaaatatc attgaagaca
    24181 ctaacaagat aatcatttac tgtttttaaa tctggttcca tgttccaccg tccactaatt
    24241 agtttgatta tcaaatcttt tgatgttcaa actatatctt aacttttttc aaaatgcaac
    24301 cttaaaagat cattttttta ctatatgagc tttttttaag attttactca tttaaaacaa
    24361 ctgataaatc agcatttata atttctttta ttagaaaaat catctatctc acatattttg
    24421 attatcaaag tattttctgt gaacagaact atagaattcc ctaaaacttg tccattattt
    24481 caaaatttgt tatagtcttt ttattcaaag cgtaaaagga gatcctatgt taaaagaaaa
    24541 aatgaaaact ctccccatgc tgccgggtgt ctatcttatg aaagaccgag atggtaaggt
    24601 catctatgtt ggtaaagcaa aacggctcaa aaatagagta agcagctatt ttcatcagaa
    24661 taaacagcat tcaaaaaaag tattacgtat gattcatcat ataacagatt ttgatttcgt
    24721 cgtagtagac acggaattag atgctttatt gctcgagtgt caattgatcc aacattaccg
    24781 tcctttttat aaccgacaaa tgaattattt cagtaattat aattatgtac atattaccaa
    24841 taagggattt gtattaacag atactcctac ggctcgaaca tacgggcctt ttcgtctcta
    24901 taaaaaaatg ccgtctattt tgcgtataat ggaagaaact tatcaaatgc cttggttgtc
    24961 tgaaattagt ttgttagctt tgcgagtcca gcttcctgac ctacaggaaa tgacttttga
    25021 acagaaaaaa aaggaactcc aagggctgtt tcaaggccga aataaaaagc tactgactta
    25081 cttgaaaaaa agacaacaac atttcatcta tcagttgaat ttcgaaaaag ccggtatgct
    25141 tcaaaaagat atcgaattag ttacttattt tattagacgg atccaggaac aaaagcagtt
    25201 tttaagaaca ccatcgctca ccttcagcat gccattagca gcagatgaga gtcagaaaaa
    25261 gcactacctc gtttgttatg gtcaacttgc agaaacaatg attacctctg gtgacgtttc
    25321 tcctgacttc tattatgaaa agaaagaagc tcatttatct ttgaaacgtc aattgtcaaa
    25381 agaagaaatc gatcctgtac aaatcttgat cagttaccgc aaaaaattag aaaaagaaca
    25441 gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag caactaaatt agttgcttct
    25501 cggcttcttt taggcatctt caacttcaaa tgtcaattat cctaaaattt attggattaa
    25561 gtcatagatt tccatcgcta cgatatcgat attatcaaat tcgtattgct gatgagtatc
    25621 tgtttcagtt aattcaaatg tatcggtcgc tttatcataa gttactgtac acttctctac
    25681 gccttctttt tcaaaacggc gaacttgcag ttcattatct gctgactcga ccatagcctc
    25741 cagtcgtttt atgattgcga ctaattgtga atgtgtcatc ggaggtcctc cttttcttat
    25801 ttctgttata ttgtatcaaa agcatagcct acttgcattg attttctcta aaaaaactcg
    25861 ttttttatga ttttttttct ggtatttttc cttcaggcca ccaaagtttg atcaaagctt
    25921 gggttccgtc attctcaaaa cctttatctg ccaactcttc atatagtttc aatgcttggg
    25981 tagttgctgg caatacaaga tccatttttt ctgcttcttc taatgcaatc tttaaatctt
    26041 tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat
    26101 aattgcttaa agaccagtta gctgcacttc cacctgaaag cgtctcaatc actttcttta
    26161 aatcaagtcc gtttttttgt gcatagacaa gcatttccgt cattcctgtc atcgtacctg
    26221 caatcatgat ctgattagcc atttttgtat gctgaccttt cccagcagaa ccatgcaatg
    26281 taaatgtcgt accaaattct ttgaataaag gcagtacttg atcataaacg gcttcttctc
    26341 caccgaccat gatcgtcagt gtaccattct ttgctcctaa gtcaccaccg gataccggtg
    26401 catctaacgc atctgcgcct ttttcttttg ctgtttttgc aattttttct gccaggcttg
    26461 gtgtacttgt agtcaaatca actaaaatat gtcctgagat atctgcagaa aaaatccccg
    26521 actgtccata gtagatttct tctacatctt tggggtaacc taccattgta aagagaatat
    26581 tactttgttc tgctaccgct tttggtgtat ctgcccagat agctccttct gttactaaag
    26641 gatcagcttt tgcttttgtc cgattataga cagctacttc gtgtcctgct tctagtagat
    26701 ggcgtgcgac agcacttccc atgacgcctg ttccgataaa accaagtttc attttttcga
    26761 cctcctaaaa atcaaaaatc cttgcttcta tgctagtata cgctagaaac aaggattttt
    26821 tcataaaaaa tgtttgaatt ttatctttta aatgaattta attttatttt gattcgttca
    26881 atcgagtctt cttcttcttt tcttcgcatc tcttcttcat gataaacaac catgtcttga
    26941 taaaattcga tcatagcttc gccgaaatgt gtagatgaaa tatcataaag tttagcttct
    27001 aaaacttttg gatctttagg aacttcactg gaaatataat caataagtgc tggtgcaaag
    27061 tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat cgaacaatcg gttcaaataa
    27121 gcatttcctt ccacgacaca aggtgttccg gctgccatcg cttctgtata agtaagccct
    27181 tgtgtttctg aagtagaagc actgacaaaa taatctgccg ctttataata aagtgctaca
    27241 tcattgttgt ccacttctcc tgtgaactgc acaaattctt ctaactgcag ttctgagatc
    27301 aattcctcta actcttctac ataaggtcct tttcctacga taactaatct ggcgttagga
    27361 taagcggaga tgacctgagg aaacccatta atcaaggctt ggatattttt ctcataagaa
    27421 atcctgctta aagaaagaat catcaaatgg ttttcctgaa gacctagtga ctcccttaat
    27481 ttgctgatat cctgctgagt gatatcagaa cgtttgaatt tttcaatgtc gataccagta
    27541 ggaatcacgc gaagcggtgc tattacacca tagctgcgta atgtatcaat aacacgttca
    27601 cttggacaaa ctacacctgt cgtatgattt gcaaataatc tagacaagta ttttacatgg
    27661 gattgcctga tcacttttcc tttaccgata taatgaaggt agtcttcata catcgtatgg
    27721 taagtatgga tcagaggtat tttcaacttc ttagctacca tcttcccaag tatccctgct
    27781 ccgaattctg tatgggtatg aataagatct agttctagtt ctttggcaac caaataagca
    27841 taccacatgc ctctaacaac aactcttcga tccttgaaag aaataaatgg tacacttggc
    27901 attcgaatca catctttttc caacttatcc gcattaggat cggtcgttgt aaaaatatag
    27961 acttcatgcc catgtctttc taattcttgt tttaatgttt tgatcgatgt agcaactccg
    28021 ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt tcatgttttt cgcctccaaa
    28081 ctagtccttg acctgattta ctttttttaa tttgctagag actttttcat tcagtacact
    28141 ttcgtataca tttttaagtt cttcaccgat tttattgata cttcgctctt ttgctgtttg
    28201 ataaccggct tgtgtcgtac tcggtagttc ttgattggta atctgttgga tcagtcggat
    28261 aaattcttcg tttgtcttcc ccatatagca gttcttgcta tcttctaacc agccattata
    28321 cactggaata tctcgaacaa gtacgttttg tttgcttgct aaagcttcta aaacaacgat
    28381 cccttctgtc tcttcataag aagggaaaaa gaatagatct gcacctgaat acgcaccttc
    28441 tatgatctct ccacgaatgt aaccaggaaa caggacattt tcaggatgat cttcttttac
    28501 aattttccgg atgttccgag gaatcgagta catgggaaca tgaccaaacc aaataaatgt
    28561 gtattcgggc atcttcttag caatttctac gaaatcaatg attcctttac gttcgaaaaa
    28621 caaaccgaca caaatgatta ctttttgatc cggagacagt ttgaaatatt ctctgaactt
    28681 ttgttctttc attggatcag ggtgatattt ctctaagtcg atcccattgg aaatagcttg
    28741 aatcggcaca cggataccat agctttccaa caactgtttt gaataaggtg ttggcgtgat
    28801 cagatagtcc gctttttggt acaatcctac cagatatttt tttacgatag gagaaagctg
    28861 gttcgatctg ataaaggaat ttcggaaatc ttcctctgta gaatgcgcat gatagacaac
    28921 tttttttcct tctcttctgg ctttattgac catattatga ctattgattc cgtatgtatt
    28981 gatgtgcagg atgtcataat cttctttttc atctagtgta taagagatac cgacttccgt
    29041 cagagcacgt ttttgatgat caagcgcacg cccgattcca gattttgcaa gcatcttttc
    29101 actttcaaaa tataataata ccttcacctt acgtccccct tcacttatat agtataacat
    29161 agcctacaaa ctgtcactca aacttaagcc ctatttttgt ctattcttac tgacaaaaac
    29221 gttttcttaa aaaagaacaa aaaaactggc gtagctcttt tacaagccac accagctttt
    29281 gatacgttgt ttattttaca tattcatgaa caagttcaac gacttcttcc atcatatcgc
    29341 attctttcaa tgcacgatca gcaagttcag ccattttcgc tgtgtccaag cgtttcatta
    29401 aactacgcgt tttaagaata gatgtcgcac tcattgagaa ctcatctaga cccataccta
    29461 ctagcaatgg aacagctgtt tgatctccag ccatttctcc acacatacct gcccattttc
    29521 cttcagcgtg tgctgcatca attacatttt tgatcaaacg taaaattgat gggttatatg
    29581 gttggtacaa gtaagaaacg cgttcgttca tacggtctgc tgccattgtg tattgaatca
    29641 aatcgttcgt acctacagag aagaaatcta cttcttttgc aaacttgtct gctaagactg
    29701 ctgctgctgg aatctcgatc atgatgccga cttggatcgt atcagatact tccttgcctt
    29761 cacttatcaa tttttgtttt tcttcttcaa agatcgcttt tgctgcgcgg aattctttga
    29821 gtgtagctac catagggaac atgatacgta aattaccatg aacagatgca cgaagcaatg
    29881 cacgcatttg tgtacggaac atttcgtcgc cttgttcaga taaactgata cgcaatgcac
    29941 gatatcctaa gaatggattc atttcatgag gtaattgcag atatggtagt tctttatctc
    30001 caccgatgtc cattgtacga acaactacag gttttccttc cataccttct aatacagctt
    30061 tgtaagcttc atattggtca tcttctgttg ggaagtctgg agaatccata tacaggaatt
    30121 cagtacggta cagaccaact gcttctccac cattattatg gacaccaact agatcttttg
    30181 gtgttccgat gtttgcagct aattcaaaat gcttgccgtc agctgtgaca gtttctgcat
    30241 tttttaattt ttcccattct gctttttgtg ctgcgtaatc tgcacctgct ttttcaaatt
    30301 cagctttttg ttcatctgta ggatcgatga ttacatcgcc ttcgatcccg ttgacagcca
    30361 aaatatcgcc ttctttgact ttagcagtaa tttcttttgt cccaacgatt gctggaattt
    30421 ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc accaatgtct gttacaaacg
    30481 cttttacaaa attgcgatct aattgtgctg tatcacttgg agtcaagtca tgtgcaacaa
    30541 cgaccacttc ttcgttgatc attgatggat ttggtaaagt aacgcctaat aagtgcgcta
    30601 aaatacgttt agcaacgtca cgaatatctg cagcacgctc ttgcatataa gcattatctt
    30661 ccattgcttc gaacataccg atatacatat ccgttacttc ttttagcgct gattcagcat
    30721 ttacgctgtt gtctttaata ttttgcttga tttgacctac catttctgga tctgaaagaa
    30781 ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc taagctttgc gctgcttttt
    30841 cacgaatttg ctgcaattct tcagtagatt tagctaatgc agcatccaaa cgagcttctt
    30901 cagccgcagt atcttctact gttgccttac tgaatgttaa atccggttga actagcaggt
    30961 aagcttttgc aacggctaca ccgtcactag cggcaatccc ttttagcatt tcaaccatta
    31021 ttcagataag ccttctttct tcattgtttc aacgattgct gccattgcat cagcttcgtc
    31081 agcaccttct gcagtaatag taacatcaga accttgacct acacctaaag acataacgcc
    31141 catgattgat ttcaagttta ctgatttacc tttatactct aagtttacat cagagttaaa
    31201 tttgcttgct gtttgtacta atagtgtagc tggacgagcg tggatcccag tttctgctac
    31261 tacgtgaaat tctttctttt ccatattaat cggtctcctt taagtgagtg ttgtttttat
    31321 gtgagggttt tcttttttag taattcagag aatcctaaca aatgccaccc tttcatctgt
    31381 aaagattaac attttttttc gttagataca acagttttca ttcaatttca gataaaagta
    31441 ttcaaattat tccggattct ttttcatcct tttcttcaca ttttataaca aacgtgttta
    31501 tttccttttt cttgtcgctt tctcttctcc tgacgtttat cattctttcc tttgaccctc
    31561 tttgttttca tatgaaacga attacttgct tcccttttta tttttagtat aataagagtc
    31621 aaaggtcagg aatggtcaat agagatttca ccttttctac ctcataaatc tcttaaaaaa
    31681 ctaggggtta ttcttttacc tcatgaaaaa cgagatgtaa aatatggatg tacccatttt
    31741 tgtgaaagga cgtgaatgat tcatgctttg tcaaaactgc ggcaagaatg aggcaacgat
    31801 tcacttgtat gccactgtca atggtcagcg gacacaactc gattactgtc agagctgtta
    31861 tcaaaaaatc aaaaatcaac aaaatggagg tctgactaat atggcccaaa atgatccatt
    31921 tggatttggc agtttggacg atcttttccg ttccatgtct cgccaaatgc aacagcaagg
    31981 aaattatgag caaacaccac caacacaatt tggcggaaac aacaacttca atggtggtca
    32041 gccacctcaa ggcaatacag aaggtttatt gggagaatac ggaatcaata tcacagaaca
    32101 agcacgacaa ggtgatatcg atcctgtgat cggacgcgat gacgaaatca aacgagtcat
    32161 tgaaatttta aatcgccgaa ccaagaacaa tcctgtcttg attggagaac caggtgttgg
    32221 taaaacagca gttgttgaag gtttagcaca aaaaatcgta gatggtgatg taccgcaaaa
    32281 attaatggac aaagaagtca ttcgtttaga tgtcgtatct ttagtccaag gtactggtat
    32341 acgaggacaa tttgaagaac gtatgcaaaa attgattgaa gagatccgtc aagcagaaaa
    32401 tgtgatccta ttcatagatg aagttcacga aatcgtcggt gctggttctg ctggtgatgg
    32461 aaatatggat gctggtaata ttttaaaacc agctttagct cgaggagaat tgcaaatggt
    32521 tggtgctact accttaaatg agtaccgcat catcgaaaaa gacgctgctt tagaacgtcg
    32581 tatgcaacct gtacgggtag atgaaccaac tgtcgaagaa acgatcagta ttttgaaagg
    32641 tttgcaaaaa cgttatgaag actatcatca tgtaaaatat actgatgagg ccatcgaagc
    32701 agccgcaact ttatccaatc gttacgtcca agatcgcttc ttgccggata aagccatcga
    32761 tttgctagat gaaacaggtt ctaagaaaaa cttgacgatc caaatcgttg atcctaaaac
    32821 aatcgagaaa aaactgcaag aagcagaaga acaaaaagtt ttagcttctc gagaagaaga
    32881 tttcgaaaaa gcagcttatt atcgcgatca gatcaataaa cttcaaaaaa tgaaagaacg
    32941 tcagctcact gaagaggaaa cacctgtgat taccgaaaaa gatatggaaa aaatcgttga
    33001 acaacggaca ggtattccag tgggtgaatt aaaagaaaaa gaacagacac aactgaaaaa
    33061 cttagcagat gacttgaaag ctcatgtcat cggacaagat aacgctgtag atcgagtagc
    33121 gaaagcgatt cgccgaaacc gtgttggatt aaacaaacag aatcgtccaa tcggctcctt
    33181 cttatttgtt ggaccaactg gtgtaggtaa aacagagctt gcgaaacaac ttgcctacga
    33241 actattcggt tcacaagatt caatgattcg tttcgatatg tctgaatata tggaaaaaca
    33301 tagtgtctct aaattgattg gttctcctcc aggttatgtc ggatacgaag aagctggtca
    33361 attaacagaa aaagtacgga gaaatcctta cagtcttgtc ttgttagatg aagtagaaaa
    33421 agctcatcca gatgttttgc atatgttctt gcaaatctta gatgatggtc gattgacaga
    33481 tgcacaagga agaacggtca gctttaaaga tacgatcatc attatgacaa gtaatgccgg
    33541 aactggtaaa gtagaagcaa acgttggttt tggtgcggca cgcgaaggtg ttactcgttc
    33601 tgtattgaac caattaaaca actactttac accagaattt ctgaaccgtt ttgacgggat
    33661 catcgaattc agtgcattat caaaagaaaa cttgatgaca atcgtcacac taatgctaga
    33721 cgatgtcaac caaatgctgg cagcacagca acttcacatc gaagttccta cgaatgtcaa
    33781 agaaaaatta gttgacttag gctatgatcc atctatgggt gctcgtcctc ttcgacgcac
    33841 gatccaagaa caaatcgaag atggtatcgc tgaattttat cttgatcacc cttctatcca
    33901 tgagttgaaa gcaaaactgg ataaagatgg aaaaatcatc gttacttcta aaccagaacg
    33961 tttggctaaa gaatcagccg aagaaacggc tgaataaatc cagttttata gcgtgagacg
    34021 gtatttcttt gagaaatgcc gtctcattct tttttcagaa aagtcaaaat actgttcatt
    34081 ttataaaaaa gaaattataa taagagaaac aagcaattaa aaatacataa aagaggtgag
    34141 cgccatgaaa ttggtaaatg taacaaacag ccattcccgt cttgtattaa accagcttga
    34201 aaacactgat gcccatctag tcaaggtcta cacagccgga aataccacta ttgtctacac
    34261 tgaagcccct gaacataatg aaattctgct gatcaatgac aaacgtaaaa tccagccgaa
    34321 agaaatcgaa gatgctaaaa attatttctt agataaaatc aaagacgccg tttaccacga
    34381 ggatgaaatc aaagtaatcg aattggcagg ccttgttgaa atctcgattc caaaacatga
    34441 attaggcgct acattagccg tgtaaacaaa ttttgaacat aataattaaa gagcgtggga
    34501 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg aaagcagaag
    34561 caactccttc ggaaataagc tgaaattcac gaaaattgct tttcaaattt ttgtgaattc
    34621 cagcttattt cttggagtta aacgcttctg tctcagcctc tttttctttc tagctttttg
    34681 atttttctct tttcttttgt acttttctct tttcattttc agttctacgc gtgtctttat
    34741 ttttaacttt ttcctcttta tggatgattg atcgtacttt ttgtgcaatt tgctcgtttt
    34801 tgtcccagtc ttttacatct aataatcgac ataatcgtaa catctcttct tttttctgtt
    34861 tttttctttc acgtctttgc cattctgttt ccatgtgtca cactctcctt tctttaatca
    34921 tatgtacgca aaataatttt tatatttttt tgattagaca tttctatgat tttaatatag
    34981 taaaaagagc cgtcacggaa aatccgtcac ggctctaaac aaaatatgag aggctgtttt
    35041 tgtttggcta ttcttattac ttgatgcagg gcagcttgtg tcaagacttc tttacatcaa
    35101 actttttaat tttacattgg gatatttatc cgcaaaccag cgttctgcaa attggttctc
    35161 gaataagaac aacggttggt cgaaacggtc acgagccaag atattgcgac ttgaactcat
    35221 cttctcatcc aaatcttctg gatcgatcca gcgagcgatt ttgtgtccca ttggagacat
    35281 gattacttcg gcgttatact catttaacat acgatattgg aatacttcga attgtagctg
    35341 accaacagct ccaatgatat attcttccgt tagatatgtt ttataaagct gaatcgctcc
    35401 ttcttgaact aattgataga ttcctttgtg gaaggatttt tgtttcatga cattttttgc
    35461 agtcactttc ataaacagct ctggagtaaa tgaagggagt tcttcataag caacttttag
    35521 ttttccttcg aacaatgtat ccccaatctg ataattgcct gtatcatata caccgatgat
    35581 atcgcctgct actgcttttt cgacgttttc tctcgaatct gccataaatt gtgtgacatt
    35641 gcttaatttc agttttttat ttgtacgttc caaccagaca tccattcctc gttcgaatgt
    35701 tccagaacag attcggacaa aagcgattcg gtcacgatgg gctggattca tatttgcttg
    35761 gatcttgaat acgaaacctg aaaattcttc ctcataagga cttaccatct gatcatcttg
    35821 tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa gtctctaaaa aagtctgtac
    35881 accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc tgatcgccac gagcaatttt
    35941 ttcttgatta aatgcatctc cagcctcttt gattagttct acttcttcta agacttgttc
    36001 atataaggaa ttttcagtta gaggatgatt ttcgggaagc agtccatctt catccaattc
    36061 tactaatcgt tcatcctgat aattttccgg acgataaaac tctacacgat tatggtagat
    36121 atcataaaga ccttctagcc ctttacccat accgattggc caattcatcg gataagattc
    36181 gatatctaat agctcttcta attcttctaa caattccaat ggttcacgac catcacgatc
    36241 tagtttgttg ataaaagtaa atatcgggat gccacgtttt tttacaactt ggaataattt
    36301 ctttgtctgt gcttcgatcc cttttgcact atcgattacc atgaccgcac tatccacggc
    36361 catcaaagta cgataagtat cttcagaaaa atcttcatgt cctggtgtat ccaagatatt
    36421 tactcgtttc ccttgataat caaactgcat aacagaacta gtaacagaaa tcccacgttg
    36481 tttctcgatt tccatccagt ctgatttggc aaaattacct gtttttttgc cttttacggt
    36541 cccggcttga cggatagccc caccaaacaa tagtaattgt tctgtaatcg ttgttttccc
    36601 agcatccgga tgggaaatga tcgcaaaagt tctgcgattg tccacttgtt gctttaattt
    36661 tggattattc ataacctaac tccatttctt ccattcaatt atttatattt tctctaggca
    36721 cagattgtcc gatctcttaa agaaagacaa tttcatatag ggctgtgaca aaagccgtgt
    36781 agcagtctct ttttccgagt aaacggcgtt ctagagacag cctatcagca ataagcccac
    36841 ccaagccaaa aatacgaaaa gctgtttttg cttggctgct cttattactt aatgcaggat
    36901 agctttttca caacctttgt caactactac aactgaatca gtatagcatg gattcagcga
    36961 atgaaaaagc caccaaacca taagagtcaa atattttttt gtcagctgac taaaagaaaa
    37021 gacgtgttgc ttgtttattc aagcaacacg tctttcataa ccaagtttcc aattatcttc
    37081 ttggctcgtc atcttcgaat tcttttctga agtatcttcg ttcttcttca ggttccgctt
    37141 ctgtttcttc cggatcatga aaaatgacac ggattttaag gattcgagaa ccttccattt
    37201 cttcagaaac cagcgtaata ttatcgacat caaacgataa cttttcgcct tcatccggaa
    37261 tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc tacatcactc atatgcagat
    37321 cactgtcaaa agcttcattg aactcatcaa tcagcatacg tccttggatg atatattcgt
    37381 gttcatcgat tttttcataa agattctcaa cttcatctga ttcatcatcg atttctccga
    37441 cgatttcttc taaaaggtct tctagtgttg ctaacccaac gacgccgcca tattcatcta
    37501 ataaaatagc catctgattc tgcgtctttt tcatttcata taacagatca tcaataaaga
    37561 ccgtttcagg aacgaataaa ggctcctgca tgattttctt gatatctaaa ttatcaaagc
    37621 cgaatttatg ggcagctttt agtaaattct ttgtatgaag gattccgact actttatctt
    37681 tgtcttcgtt ataaacaggg attcttgaat agttttcgga taaaacttca ttcacatttt
    37741 cttccacagt gtcgttgata tcaatcataa atgcatccgt tcgtgggacc atgacttctc
    37801 gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat ttctaattct tcattattca
    37861 atacgccttc gttttcaagc atataacgca tttcatctct ggtcattttt gaatcttcat
    37921 catcaaaagt catcggcgtg atacgcgcca ataaacttgt ggaagcagaa agcagccaga
    37981 cgaagggctt agctactaca ccaatcaagc gtatcagccc tgaagtgaac tttgcgactt
    38041 cttctgattt atttaaagca atacgtttag ggtacaattc tccaaaaaca atcgatacat
    38101 aggtcaaaat agccagcaca atgatatttg caatattttt tgccgctgtg ccgccaccta
    38161 ataccggagc taaccgttcc gataaagtat tagctagtga agcaccagac aaaatgttga
    38221 ccaaagtgat tcctacttgg attgtcgata ggaaattatt tggattctgc aatattttta
    38281 aaagcttttg agatttcaca tctccttcat cagctttttg ctcgattcga ttcttattga
    38341 tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt gatcaatgtc aaaacgatca
    38401 ataataaaat ctgcgctaac agcgactgac tctcagggtc agcattcata atcgcattct
    38461 ctccttataa aattaatttt tagtttacga tagaaaaaaa cgtggcacta tagccacgtt
    38521 aaaatggtat cacaaagcaa ttttctttgc aataaaaaca agtgtactaa aactacctac
    38581 gaggtcgtag caattttctt cttgttttca ttttgcttgt cttctttgac gattttgatt
    38641 atataggaaa tgatcgtctt gcagattgcg taaaaaggaa cacctaagaa gataccaagc
    38701 aatccggcaa tatttccagc tactagaaga acgataatga tcgttaatgg atggattttc
    38761 agtgatttcc caattacatt aggataaatg atattcccat ccaactgttg gacgattagc
    38821 acaaccacac aacaaagtaa tgcttttacc ggatcattga agactgttac tagaaatgca
    38881 ggtgctagtc ctagataagg tcctaaatat ggaattaggt ttgtcaatcc cgcaatgacc
    38941 ccaaataaga aagcataatc cacaccaatt accatatatc ctaaaaatgt gaaggtacca
    39001 acgaataaac attcgattgc ttgtccgcta atgtaattag ccaatgtttt attcagttgc
    39061 cccaataact ccacaatttg gtttcttctt ttttctggga aaaactgttg gatgtttggc
    39121 actagttttt ctccgtcttt caacatatag aacaagatga atggtgctgt aatgattact
    39181 acggttgccg aagcgatcgt tccaacgatc gaacctaaac tattggatac tccactaagg
    39241 aattgctgaa tgattgtccc gtaagaaatg tctaattgct caaaatattt attgatatct
    39301 acttccttga acaaaggcaa ttcggctacc tcttttaacc agctttctac ctgtttgatg
    39361 aaatcaggca tattagatgc caatgaggaa atttgctgta ctagacttgg gatgacacta
    39421 agtaaaatcc aaataattgc tgcaactaaa aggagcagca caattgcgac agcccatatt
    39481 cttttgattt ttgctttcat caacagcttg acaataggat tcaataaata gtaaagaaag
    39541 cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg tcccgatcgg ttcgaaaata
    39601 aagtttattt ctgatgaaat catgatcaat gcagctatga tcaataattc aagcgaccaa
    39661 aacattagtt tagactgtct aagtttttca aacaatagat ttcctccttt ctttcttact
    39721 agtatatatt atattcgtta aaaattcttt tattcttttt ttatttttga aaacttgcca
    39781 tactttttat ttgttttatt ttccagtata ctatacgaaa ggaaggtggc tagaccatga
    39841 aagttgtaca aacaaaagat acaatgagca atatttactt agatgctgta cggatcaggc
    39901 atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg agaaatagac aaagatgaag
    39961 cccattgtat ccacttcgtc ctgtactcag ataaaaaaga accgcaagga actgtacgtc
    40021 ttttgccttt agaaaacgga aaaatgaaat tacagcggat ggcgattctt tctgaataca
    40081 gacaccaagg gttaggaaaa atattgattg aagaagcaga aaactttgct aagaatcaag
    40141 gctataatac gattttatta ggggcacagt ccacagctga aactttttac gaaaaactag
    40201 gatatacagc ttacggcgat ccttttgaag atgctggcat gcctcatatt tatatgaaaa
    40261 agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg agaaaaatct
    40321 ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg taacggttat
    40381 accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc cagtcaacca
    40441 gaatcttcac attttctttt aggatgaagt caatatcgtt cgggtaattc atcattttgc
    40501 tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga aataccttga
    40561 tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa ggtgaagcta
    40621 gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg aaccagtatt
    40681 ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca tcagattcag
    40741 tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt aataccatgg
    40801 tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc gtcacaaact
    40861 ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt gctttgtgtt
    40921 tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc tttgcctaga
    40981 aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt tggaccttag
    41041 gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg ttctcggtaa
    41101 attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc catttcagat
    41161 gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag agttcctgct
    41221 tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga tcgatttctg
    41281 gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact tccatcattg
    41341 gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa taatacgctc
    41401 cagaatgatt ttgaagagct gcactgatat aataaacgtc ttttggcttt gctttttttg
    41461 tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag gcttggatcg
    41521 gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga tccatcattg
    41581 cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct tcatcaaaga
    41641 ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg ctgactactc
    41701 gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa tgcgatactt gcaatcgcgc
    41761 ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt tgcggcattt
    41821 ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg gctcttgagt
    41881 aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt gctagctctt
    41941 caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg tctactctgg
    42001 tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta tagcgccaag
    42061 gcaggtttct tttttcttgt tcataccatt gaatgaactg atcttgaaat tcttttgttt
    42121 ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc cgtaatgaat
    42181 cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac ttttagattt
    42241 ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg tttgactaaa
    42301 taatcatatt ctatttcttg atctgcttct tgcggaagat cagctatcgc ttgttggatc
    42361 acttcttgag aaaagccttt cgtcatcaat gtttgttgga tttttctcaa taattctttt
    42421 tggctttttc cgtgattttt tccaaacgct ttttcggctg tctgatgtgc attttgaact
    42481 tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt caaacctttt
    42541 tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat attcgtgcgc
    42601 acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa gcgggctacg
    42661 attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg gatttctttt
    42721 tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa atcagctccg
    42781 ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt taataatcga
    42841 tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa atctacgcga
    42901 tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat cctcctcgaa
    42961 catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat gttagaataa
    43021 accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa aactggacaa
    43081 acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg ttatttcaaa
    43141 cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt gaaaatcacg
    43201 aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac aagcaaagat
    43261 cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt acaacactta
    43321 gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt ggaaaaattc
    43381 aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga tgagccttgg
    43441 cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca aaaaattgaa
    43501 gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg cttggttcaa
    43561 gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa atttgatgca
    43621 cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt tagaatcggt
    43681 gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt cccgcaaaaa
    43741 aacgcaatga tccgtgagat caatgaacga ctgcccgaag ttgtttcaat catgcaaaat
    43801 gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt atggggaaaa
    43861 gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg tgccttcttt
    43921 caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc attagatgtc
    43981 cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg cttaagtgta
    44041 gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat cgctgatgca
    44101 aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg gacagcagag
    44161 caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat cgtagatcca
    44221 ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc tccaaaaaaa
    44281 cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa tttaagtaaa
    44341 aagttcaaga ttgaatattt acagtccgtc gacatgtttc ctcagactgc acgttgtgaa
    44401 gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa aaagtctgcc
    44461 ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta tgaatactgt
    44521 acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg ttgtgacata
    44581 gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac ttgataaaat
    44641 gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc ttttcgatcc
    44701 tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt gtcgtctatt
    44761 tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca agttggtttg
    44821 gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca agtaatattt
    44881 aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc atctaaaggc
    44941 tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt tttggtagct
    45001 tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt tcctgctgct
    45061 gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc aaaacctgga
    45121 atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt actaatcttt
    45181 ttcatgtaga aacctccttc atttgataac cacattgtaa acgctttatt gtattaacac
    45241 aaacattatg cttttataat tttatgttga agtaaaaaag gtgaaaaaag taagatgcag
    45301 attataacag aagtagacga gaaaagtaca aatagaaaac atgagaatca aatcatatga
    45361 tctttttcaa aaacatcatt catataaaca aatgttcaac tttttaataa caacaattat
    45421 caattttttt cttacttcac aaactaaata caggtcttgc tgtgagcctt catggtaaac
    45481 taatattata gaaaggagtt agtaaaatga agatgataaa agatattcgt ttttgggttt
    45541 gtgttatagg aattgtcata ttaggctttt tatcagggtt gcttagcgga aatcccggaa
    45601 aatattacta ttctttgcaa cttccaccat ttgcaccacc tagctggata tttgggccga
    45661 tgtggacctt actgtatata ttgatgggta tttctcttta tttgttactt aatcacaaca
    45721 acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat tcagttcatt tttaatttta
    45781 tatggtctgc tctatttttt aatttaagaa atattttcat tgcagcaatc gatattacac
    45841 ttttagttat tttcttgtca gtgcttatgt atcagttgtg gctgcatcat cgcctggcaa
    45901 tgtggctcat gattccttat tatttatggg tactattcgc aactctattg aattattcaa
    45961 tttatttttt aaactaagcg ctaaaaagag acgtcctgta atttaacaca tagaaaaagt
    46021 gccacctaag ttagattttt tgtctaattt aggtggcact ctttattaac caaactattt
    46081 ttttctagta cccacttgtt gttccatact cattattctg gtcataacca taaccatttt
    46141 gttcctgtcc agcgtaagga tcataacttt gagtatcttg acttggtgta tagtactgac
    46201 ttgtatcttc tgtttgctga taataggtat tcggatcttg gtactgatca tatgtgtttt
    46261 ctgaatagga agaataatcg gtgtaccctt cactattcga tccatcaccg tttgtcgaat
    46321 cgccatcttc cgaatcatca aagaacatca ttgaagcatt ctcatttttc aaattaggta
    46381 atgtatcgct tgtttttata tttaattgtt tcttcagttc attttggatc gaaagaagat
    46441 cattttttcc taaaatttga tagtagatat cattgatcat ttgtccttcg ccttgcaact
    46501 ggtcttgttt gattgtttca aacgccgggg tgtagttcgt cgctacatca agcatgtcat
    46561 cccaatctaa gtccgtcgtc acattgttct caactgcttt taaaattttt ctataattgc
    46621 ttacgccatc taaacttaaa actttattga cgattttcgt cactacttcc cgctgacgac
    46681 gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat tcgagaatag gctaaacctt
    46741 tttctccatt caatgtttgt tcacctactg gtacatggac accatccaaa gtaaagtcaa
    46801 ttttattatt cactttgatg ccaccgaccg cattgatcaa atcttccatg ccatccaaat
    46861 tgatcgttac ataatgatca ataggaatat cgagcagttg ctcaattgaa tccatggcca
    46921 tttcgacacc accaaatgcg taggcatgat ttaatttatc taccgttcca taacctacga
    46981 tattcgtata aatatctcgg tccaaactga tgatcgttga ttttttttgt ttcggattaa
    47041 ctgttactac catcatcgta tcagaacgtc cttgttccgt tcttcctaat ccaccggtat
    47101 ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc aatgcttact ctctcagaac
    47161 gtttggagga tattcgatta gagtttttag aaattttgtt tactgtttgg ctcgcttctc
    47221 ccataaatct gattccgtat gctgataatc cggcaatcag caaaacgaat acccctaaca
    47281 aggttaatag aactttttgc catcgcttca taatacacct ctgtttcaca ttttctaaaa
    47341 tattgtctca tataactgtt caatcagcaa attcactttt taatcataag aaaactttaa
    47401 aaaacatttt tgtgtaacga ttattatttt ttaaaatttc tcaaaattct taactatcat
    47461 accataccgt gaaaaagatc gaaagaccga atacacgcag ttcgcaatcg ttttatcttt
    47521 ttttatgtat tttttccaga aaaagcgaac aacaaggtat tttccaaata atcgtgcagt
    47581 aaaatgatcg cacatacctt tcccatcttt ttaacagtct ttgcctaccg ttcagattat
    47641 cattcgattt cacgacaatt tcttcctatg actggtcctt ttgttaaacc catcttctat
    47701 tcgttttatt tccagctcac tgcatggctc ttaaaatgac tcgaactagt gattgcatta
    47761 ccatcatctt ttaatggata gatatgctcc tcttcaaagt aaaacgattt tcttttaaag
    47821 taatagaatt gacatggtaa tgagtatgta tactagtggt ttcacaaatg gtcttttatt
    47881 caacatccaa aaactgaaat ttctttaaaa tccattacta acgaagaaaa tgttccttgc
    47941 ttgataatta gttaggacat gtcaagtgaa caaatgcatt tacttagcca aaaaatctaa
    48001 aaacttcata aatagacaaa aagacacgac tctctaagaa gtcgtgtctt tattcaacaa
    48061 attattataa tttagtcact tcagcagctt gaggtccgcg tgcaccttca acaatcgtga
    48121 attctacttc ttgaccttct tctaggctct tgaagccgtc tccttgaatt gctgagaaat
    48181 gaacaaatac gtcatctccg ccgtctactg taataaaccc aaatcctttt tcattgttga
    48241 accatttaac cgttccgtgt tccataaatg tatatcctcc aaaaaagttc ttgcaagtaa
    48301 ccttgcaaga acattatacc caatttagaa aacgctagca agctttcgct cttttatttt
    48361 ccaaattatt tttaagactg atatgacgga ggaaatggtt attttgctgc ttcgaaacgt
    48421 ttgtttactt catcccaatt aataacattc cagaatgctg caatataatc tggtcggaca
    48481 tttttatatt tcaaataata agcatgttcc caaacgtcca aacctagaat tggtgtcttt
    48541 ccttccatca atggagaatc ttgatttgca gtagaggtaa tagctaattt tccattttcc
    48601 attacaagcc aagcccatcc agaaccaaat cgtccagcgg ctgctttttt gaattcttct
    48661 ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt cttttatttc tcctgtaggt
    48721 tcgccaccag catttggtgc catgatttcc cagaaaaatg aatggttagc atgtccgcca
    48781 ccattgttac gtacagctgt cttgatatct gttggaatag cgtccatatc agataataat
    48841 tcttctattg ttttttcgcc taattctggg tatttctcaa ttgctgaatt taaattcgtc
    48901 acataagtat tgtgatgctt atcatgatgc agatgcatcg tttcttcgtc aatataaggt
    48961 tccaatgcgt catatgcata aggtagatct ggtaatgtgt aagtcataaa aaaattcctc
    49021 ctcaatgttt tcttaataat gtctacatta caaagagtaa caaagagaag gaaattcttc
    49081 aattgttatg cctagtcgtc tttttcactt tgacctactt cttcttcaaa aacctcagtt
    49141 tctttaaaaa taaacaactt gctgaaaata tagttcgcaa ggataacaat aatttgagtg
    49201 atgattttcg ccacatactc attgctgttc ataacattga tcaacaatac cattgccccc
    49261 atgtccaatc cgtaagaaag gatacggtag aagaagaact taccgcattc aataagtgca
    49321 tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt tgttcgttac aaaagcaaac
    49381 aatacagaac aaaaccacga aatagaattg gataaggcca tacttaaatc aaaatattgg
    49441 cgtgcgagag caaaactaac aaaatttaca atcgtagcca aaccaccaaa aaagagatag
    49501 actagtatct cccaatatcc ttttgcttct aaatatcctt ttaactgtcg atactgcttc
    49561 atgagccaac ctaccttatt aaaaattctt atttatcata ctctatattt caataacttt
    49621 catctttatt taaaaaaatt aatttttttt gatcctttgc ctttgtctct gcttggatcg
    49681 tcacatgtgt aataccatat tttaatttta ataaagaaat tatacgagga tatatctttt
    49741 caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt
    49801 cgctatactg ccaaatatgg acatgatgaa catcctttac cccatcgatt gcttcaattt
    49861 cttgttccat tttttcatag tcgatagcag gtgcactttg tatcaaaata gcggcagctt
    49921 ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg
    49981 gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg
    50041 aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg
    50101 cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata actgctactg
    50161 ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg
    50221 ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta
    50281 aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc
    50341 cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag
    50401 acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa
    50461 ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct
    50521 ccattttatt tatattggat agtctttaga caagaataac atttttttga tatttcacca
    50581 atcattcagc tgtttcctta aagcacttgt atttcacttg atacttcgct agaattgcaa
    50641 gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct
    50701 ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct
    50761 atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct
    50821 taaacaacat tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc
    50881 aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta
    50941 tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg
    51001 ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag
    51061 gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt
    51121 taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt
    51181 ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca
    51241 ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta
    51301 cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa
    51361 caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa
    51421 tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa
    51481 agacaactcg ttatttctac ttcaatagac ccttttagag gttgtgacat ttttttgtca
    51541 cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag
    51601 ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa
    51661 gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag
    51721 cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca
    51781 gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg
    51841 tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata
    51901 ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa
    51961 tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat
    52021 ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa
    52081 ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg
    52141 gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac
    52201 taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa
    52261 caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca
    52321 atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc
    52381 aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc
    52441 tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc
    52501 aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc
    52561 ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa
    52621 aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa
    52681 gtgataggtt ggctcctcca ggttgggatt ataatcaaca agtaaatcat atcctttaaa
    52741 gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca ctttctttac
    52801 aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt
    52861 gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc
    52921 aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt
    52981 ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac
    53041 cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg
    53101 atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc
    53161 taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc
    53221 tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa tccttattcc
    53281 catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta ctctggcatg
    53341 atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta ccatcagagt
    53401 aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat cgaaatttct
    53461 tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag catgagtggc
    53521 tatattcata tattcattga cccgatcttc attactatca atagccagta cttcttgacc
    53581 agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta caattgcaaa
    53641 attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac aaagccttta
    53701 caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct
    53761 cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttgaatgcat
    53821 agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg gaaacatgtg
    53881 tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta ctttttcctt
    53941 cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt aatacaggct
    54001 catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct aatactcgct
    54061 cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag gtcttcaaca
    54121 aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca ataaaactgg
    54181 ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt gattcgatac
    54241 catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt gtcatcgcct
    54301 tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct tcttgaatag
    54361 ataaataaaa agagacgcct aagcgcacca tttcatggtc atctactaat aaaactttta
    54421 tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt gtaccttgac
    54481 ctttaaaact aatgattttt actgttccac caataccagc tactcgttct ttgatattgt
    54541 tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg tcgtcaataa
    54601 tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca ttggctttag
    54661 catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg
    54721 aactagatag ttttacatct tctacatccc atttcagcga aatctggatt ttattttgca
    54781 attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa ttgactggac
    54841 gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgctacca
    54901 ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc tccgtcaatg
    54961 ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc agctcacggg
    55021 ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct acatattggg
    55081 gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct tttatcttag
    55141 tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg atcggaagaa
    55201 gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct tcgattttcc
    55261 cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct acacctaagg
    55321 aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc agtaattctc
    55381 cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat agaataataa
    55441 ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt cccatcatat
    55501 tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc tacgagggtt
    55561 ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa attgctcttc
    55621 ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta cgcccaaagg
    55681 tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat catcttttgg
    55741 caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga tgttgatatc
    55801 gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct gtcttttccg
    55861 ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt tctttcgcca
    55921 aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac caataaataa
    55981 aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa aaaaaatgac
    56041 caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt tgcgcatagc
    56101 tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat tgtttacgat
    56161 ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac gccaagaact
    56221 attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat ttttttaact
    56281 gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac acaaagagaa
    56341 aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt cagactacgc
    56401 ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa tagttacttc
    56461 atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg aaattttgcc
    56521 agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt ctccatctgg
    56581 tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta caccgtcgtt
    56641 atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc cttcaacaaa
    56701 tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc cgaaaccgcg
    56761 ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt cttctaattc
    56821 tttttctaat ttttctttac cttctagagt cattggataa actttctcta ccatgtgtaa
    56881 cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata tagtccaaaa
    56941 aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca atgtctagag
    57001 cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt cttcataagt
    57061 tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat agtcattttc
    57121 tgctggttct aatactgcct tgattcctct tcgcttggat tgtcaaaagg tccaggtcca
    57181 tagccggtat tggtatacaa attatatggt gaatctacag ctgtatcttc atacgtcact
    57241 aattctttgt gttcacctaa tgcgtataaa acagagatat ctgactgaag tggcatattt
    57301 gctttgattc ggttaaagaa gacttgtgca atgtttttac gatcattttc ttttacgcct
    57361 tctttttcaa ctagagaagc tagagttaaa acttcttgta cagtcaaatt tttctgcttg
    57421 atagctgaat aatagttttg catgaccgta ttcgtcttgt tcaccatttc aatcactaga
    57481 tccttaagcg aagtactcgt gtaataatca tatgtggctg ggaataagta accttccaaa
    57541 cggtattttg tgttctcagc tttagatgca ctttccaaca gttctgggaa tttttggtgc
    57601 aactcgttaa agaaagtctc atcattcact aaatctaaaa attctttctt gtctttacca
    57661 gttaccttcg ctacccgttc agcaatctga tcgatatcgt acccttctgg aatggcaatt
    57721 ttcccatctg caacttttgt tggctcagaa gtaccgcctt cttgcaactg tttgccaatc
    57781 tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt
    57841 ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa
    57901 atctcaccaa tctgcttgtt agaagagcca ctcgggatct cgacttggac taattggtca
    57961 tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga
    58021 aatccgccaa taatcaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta
    58081 tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg
    58141 gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac
    58201 tatcaagttc gtaactttat tcattataca tgatatttga attggcgtga atgattttat
    58261 ttaaatattc tatcaaaaat gtaagtagta aatccattac tataagatta aaataaatgt
    58321 tatcaagata caagctattt tagctttatt ctttctgtaa aaaagaaagg agactaaata
    58381 aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt taatgatcgt
    58441 tgtcgtgttg tttagcctta gtgcgttctc tacaggaact attcttggat ggcaatttta
    58501 caataatgaa cactctcaga tagttgccca tgctcaatca gctcaaaaaa agttgatact
    58561 gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta
    58621 tattggctca atttacgtaa agaaaaataa tcgcatcatc atggaaaaag gctatggcta
    58681 tgcgaataaa ctcacgcaac aaccaaatga tccttctctt tattatcaga ttggttcggt
    58741 tcaaaaagca ttgactactc ttttgatttt aaaacaagtc gagcgtgggc acctttcttt
    58801 agatactaag ctggctcaat tctatccaca aattgctggc agccagcaaa ttactatccg
    58861 tgacttgctg tatatgcgct caggcttgca ccgaactgct tctccaacca taccgatgac
    58921 tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa tttacagatt atcagacctc
    58981 tcattatgat tcattgaact ttaccatatt gactggaatt ttgatcaaat taacaaatca
    59041 accgtatgaa aaattattga ccgatgatat catcgaacct ttaggattac agcatacaaa
    59101 attttatgac caagtcaaaa attcatccaa tcatgcacta tcctatcaaa tgtctaaaga
    59161 ggatgattac tttaaaccac tgacagaatc tgaaacagag atccataacg aattagggac
    59221 tggtaatatc agtatgtccg tctatgattt aaatacgctg tttacacaag ttttatctgg
    59281 aaaactagtt tccaaagact tactcttctc attatggcgt gaaaatgcga atggacatcc
    59341 atatagtggt ggggtatata gcggagataa ttatatcctg tcgcaaggaa atatcgactc
    59401 ttttgataca tctgctatat ttagcaaaga ttcacaggat gctatcatca tggaaagcaa
    59461 tataaaagct gacaaaacta tcaaactgcc tactgctgat ctaagaaacc aaatctatga
    59521 cctgatggaa ggaactacta agttggcaaa tagctgatat gatgaaaaga tagcaaatac
    59581 tacttctttg aatagcattt gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg
    59641 ggacaaatct aaaaatcaat ttttgctaca gactttaatt ccaaataaac agaacagaaa
    59701 gaggtgttct tctttctgt
//
LOCUS       DAYYCM010000009        59422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000009 DAYYCM010000000
VERSION     DAYYCM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.009"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..239
                     /locus_tag="ACHAY1_000647"
     CDS             9..239
                     /locus_tag="ACHAY1_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8592669.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            336..1637
                     /gene="murA"
                     /locus_tag="ACHAY1_000648"
     CDS             336..1637
                     /gene="murA"
                     /locus_tag="ACHAY1_000648"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8592670.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1650..1826
                     /locus_tag="ACHAY1_000649"
     CDS             1650..1826
                     /locus_tag="ACHAY1_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8592671.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1961..2257
                     /gene="yidD"
                     /locus_tag="ACHAY1_000650"
     CDS             1961..2257
                     /gene="yidD"
                     /locus_tag="ACHAY1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8592672.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2442..2909
                     /locus_tag="ACHAY1_000651"
     CDS             2442..2909
                     /locus_tag="ACHAY1_000651"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592673.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2994..3878
                     /locus_tag="ACHAY1_000652"
     CDS             2994..3878
                     /locus_tag="ACHAY1_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8592674.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3992..4228
                     /locus_tag="ACHAY1_000653"
     CDS             3992..4228
                     /locus_tag="ACHAY1_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8592675.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4316..4396
                     /locus_tag="ACHAY1_000654"
     tRNA            4316..4396
                     /locus_tag="ACHAY1_000654"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4350..4352,aa:Tyr,seq:gta)
     gene            4403..4474
                     /locus_tag="ACHAY1_000655"
     tRNA            4403..4474
                     /locus_tag="ACHAY1_000655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4435..4437,aa:Gln,seq:ttg)
     gene            4599..4769
                     /locus_tag="ACHAY1_000656"
     CDS             4599..4769
                     /locus_tag="ACHAY1_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592676.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            4989..5696
                     /locus_tag="ACHAY1_000657"
     CDS             4989..5696
                     /locus_tag="ACHAY1_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8592677.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5755..6399)
                     /locus_tag="ACHAY1_000658"
     CDS             complement(5755..6399)
                     /locus_tag="ACHAY1_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8592678.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6525..6812
                     /locus_tag="ACHAY1_000659"
     CDS             6525..6812
                     /locus_tag="ACHAY1_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592679.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6920..7240
                     /locus_tag="ACHAY1_000660"
     CDS             6920..7240
                     /locus_tag="ACHAY1_000660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8592680.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7321..8634
                     /locus_tag="ACHAY1_000661"
     CDS             7321..8634
                     /locus_tag="ACHAY1_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8592681.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8631..9350
                     /locus_tag="ACHAY1_000662"
     CDS             8631..9350
                     /locus_tag="ACHAY1_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8592682.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9478..10029
                     /gene="raiA"
                     /locus_tag="ACHAY1_000663"
     CDS             9478..10029
                     /gene="raiA"
                     /locus_tag="ACHAY1_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8592683.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10251..12785
                     /gene="secA"
                     /locus_tag="ACHAY1_000664"
     CDS             10251..12785
                     /gene="secA"
                     /locus_tag="ACHAY1_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8592684.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12911..14012
                     /gene="prfB"
                     /locus_tag="ACHAY1_000665"
     CDS             join(12911..12982,12984..14012)
                     /gene="prfB"
                     /locus_tag="ACHAY1_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8592685.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14117..14803
                     /gene="ftsE"
                     /locus_tag="ACHAY1_000666"
     CDS             14117..14803
                     /gene="ftsE"
                     /locus_tag="ACHAY1_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8592686.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14796..15680
                     /gene="ftsX"
                     /locus_tag="ACHAY1_000667"
     CDS             14796..15680
                     /gene="ftsX"
                     /locus_tag="ACHAY1_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8592687.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            15832..17280
                     /gene="cls"
                     /locus_tag="ACHAY1_000668"
     CDS             15832..17280
                     /gene="cls"
                     /locus_tag="ACHAY1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8592688.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17617..18474
                     /locus_tag="ACHAY1_000669"
     CDS             17617..18474
                     /locus_tag="ACHAY1_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8592689.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18589..19509
                     /gene="pstC"
                     /locus_tag="ACHAY1_000670"
     CDS             18589..19509
                     /gene="pstC"
                     /locus_tag="ACHAY1_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8592690.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19509..20393
                     /gene="pstA"
                     /locus_tag="ACHAY1_000671"
     CDS             19509..20393
                     /gene="pstA"
                     /locus_tag="ACHAY1_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8592691.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20408..21214
                     /gene="pstB"
                     /locus_tag="ACHAY1_000672"
     CDS             20408..21214
                     /gene="pstB"
                     /locus_tag="ACHAY1_000672"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8592692.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21230..21988
                     /gene="pstB"
                     /locus_tag="ACHAY1_000673"
     CDS             21230..21988
                     /gene="pstB"
                     /locus_tag="ACHAY1_000673"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8592693.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22001..22678
                     /gene="phoU"
                     /locus_tag="ACHAY1_000674"
     CDS             22001..22678
                     /gene="phoU"
                     /locus_tag="ACHAY1_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8592694.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23255..23947
                     /locus_tag="ACHAY1_000675"
     CDS             23255..23947
                     /locus_tag="ACHAY1_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8592695.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            23944..24711
                     /locus_tag="ACHAY1_000676"
     CDS             23944..24711
                     /locus_tag="ACHAY1_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8592696.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24811..26379
                     /gene="liaX"
                     /locus_tag="ACHAY1_000677"
     CDS             24811..26379
                     /gene="liaX"
                     /locus_tag="ACHAY1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8592697.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26676..26996
                     /locus_tag="ACHAY1_000678"
     CDS             26676..26996
                     /locus_tag="ACHAY1_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8592698.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            26996..27346
                     /locus_tag="ACHAY1_000679"
     CDS             26996..27346
                     /locus_tag="ACHAY1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8592699.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27513..28451
                     /gene="hprK"
                     /locus_tag="ACHAY1_000680"
     CDS             27513..28451
                     /gene="hprK"
                     /locus_tag="ACHAY1_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8592700.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28466..29296
                     /gene="lgt"
                     /locus_tag="ACHAY1_000681"
     CDS             28466..29296
                     /gene="lgt"
                     /locus_tag="ACHAY1_000681"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8592701.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29340..30365
                     /locus_tag="ACHAY1_000682"
     CDS             29340..30365
                     /locus_tag="ACHAY1_000682"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8592702.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30380..31318
                     /gene="galU"
                     /locus_tag="ACHAY1_000683"
     CDS             30380..31318
                     /gene="galU"
                     /locus_tag="ACHAY1_000683"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8592703.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31443..31883
                     /locus_tag="ACHAY1_000684"
     CDS             31443..31883
                     /locus_tag="ACHAY1_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8592704.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31886..32488
                     /locus_tag="ACHAY1_000685"
     CDS             31886..32488
                     /locus_tag="ACHAY1_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8592705.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32547..33653)
                     /locus_tag="ACHAY1_000686"
     CDS             complement(32547..33653)
                     /locus_tag="ACHAY1_000686"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8592706.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33863..34864
                     /gene="ccpA"
                     /locus_tag="ACHAY1_000687"
     CDS             33863..34864
                     /gene="ccpA"
                     /locus_tag="ACHAY1_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8592707.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35201..37546)
                     /locus_tag="ACHAY1_000688"
     CDS             complement(35201..37546)
                     /locus_tag="ACHAY1_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8592708.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37825..38727
                     /locus_tag="ACHAY1_000689"
     CDS             37825..38727
                     /locus_tag="ACHAY1_000689"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8592709.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            38940..40715
                     /locus_tag="ACHAY1_000690"
     CDS             38940..40715
                     /locus_tag="ACHAY1_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8592710.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40781..42367)
                     /locus_tag="ACHAY1_000691"
     CDS             complement(40781..42367)
                     /locus_tag="ACHAY1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8592711.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42618..42842
                     /locus_tag="ACHAY1_000692"
     CDS             42618..42842
                     /locus_tag="ACHAY1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8592712.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            42983..44734
                     /locus_tag="ACHAY1_000693"
     CDS             42983..44734
                     /locus_tag="ACHAY1_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8592713.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44734..46518
                     /locus_tag="ACHAY1_000694"
     CDS             44734..46518
                     /locus_tag="ACHAY1_000694"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592714.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46844..47695
                     /gene="licT"
                     /locus_tag="ACHAY1_000695"
     CDS             46844..47695
                     /gene="licT"
                     /locus_tag="ACHAY1_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8592715.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47791..49671
                     /locus_tag="ACHAY1_000696"
     CDS             47791..49671
                     /locus_tag="ACHAY1_000696"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8592716.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49711..50613)
                     /locus_tag="ACHAY1_000697"
     CDS             complement(49711..50613)
                     /locus_tag="ACHAY1_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8592717.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50844..52247
                     /locus_tag="ACHAY1_000698"
     CDS             50844..52247
                     /locus_tag="ACHAY1_000698"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8592718.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52247..53680
                     /locus_tag="ACHAY1_000699"
     CDS             52247..53680
                     /locus_tag="ACHAY1_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8592719.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            53825..54751
                     /gene="trxB"
                     /locus_tag="ACHAY1_000700"
     CDS             53825..54751
                     /gene="trxB"
                     /locus_tag="ACHAY1_000700"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8592720.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            54965..56692
                     /locus_tag="ACHAY1_000701"
     CDS             54965..56692
                     /locus_tag="ACHAY1_000701"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8592721.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56707..57057
                     /locus_tag="ACHAY1_000702"
     CDS             56707..57057
                     /locus_tag="ACHAY1_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8592722.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57149..58009)
                     /locus_tag="ACHAY1_000703"
     CDS             complement(57149..58009)
                     /locus_tag="ACHAY1_000703"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8592723.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58101..58529
                     /locus_tag="ACHAY1_000704"
     CDS             58101..58529
                     /locus_tag="ACHAY1_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8592724.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58691..58867
                     /gene="rpsU"
                     /locus_tag="ACHAY1_000705"
     CDS             58691..58867
                     /gene="rpsU"
                     /locus_tag="ACHAY1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8592725.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58894..59340
                     /locus_tag="ACHAY1_000706"
     CDS             58894..59340
                     /locus_tag="ACHAY1_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8592726.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19331 a        10319 c        12790 g        16982 t
ORIGIN      
        1 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
       61 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      121 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      181 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      241 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      301 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      361 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      421 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      481 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      541 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      601 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      661 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      721 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      781 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
      841 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
      901 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
      961 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1021 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1081 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1141 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1201 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1261 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1321 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1381 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1441 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1501 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1561 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1621 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1681 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1741 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1801 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     1861 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     1921 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     1981 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2041 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2101 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2161 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2221 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2281 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2341 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2401 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2461 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2521 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2581 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2641 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2701 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2761 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     2821 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     2881 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     2941 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3001 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3061 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3121 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3181 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3241 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3301 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3361 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3421 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3481 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3541 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3601 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3661 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3721 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3781 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     3841 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     3901 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     3961 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4021 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4081 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4141 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4201 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4261 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4321 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4381 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4441 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4501 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4561 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4621 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4681 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4741 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4801 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     4861 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     4921 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     4981 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5041 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5101 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5161 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5221 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5281 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5341 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5401 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5461 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5521 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5581 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5641 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5701 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5761 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     5821 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     5881 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     5941 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6001 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6061 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6121 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6181 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6241 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6301 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6361 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6421 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6481 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6541 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6601 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6661 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6721 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6781 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     6841 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     6901 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     6961 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7021 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7081 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7141 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7201 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7261 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7321 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7381 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7441 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7501 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7561 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7621 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7681 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7741 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7801 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     7861 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     7921 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     7981 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8041 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8101 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8161 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8221 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8281 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8341 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8401 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8461 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8521 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8581 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8641 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8701 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8761 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     8821 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     8881 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     8941 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9001 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9061 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9121 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9181 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9241 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9301 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9361 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9421 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9481 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9541 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9601 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9661 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9721 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9781 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
     9841 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
     9901 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
     9961 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10021 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10081 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10141 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10201 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10261 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10321 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10381 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10441 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10501 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10561 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10621 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10681 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10741 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10801 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    10861 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    10921 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    10981 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11041 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11101 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11161 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11221 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11281 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11341 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11401 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11461 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11521 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11581 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11641 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11701 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11761 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    11821 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    11881 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    11941 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12001 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12061 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12121 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12181 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12241 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12301 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12361 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12421 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12481 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12541 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12601 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12661 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12721 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12781 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    12841 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    12901 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    12961 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13021 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13081 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13141 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13201 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13261 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13321 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13381 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13441 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13501 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13561 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13621 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13681 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13741 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13801 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    13861 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    13921 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    13981 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14041 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14101 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14161 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14221 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14281 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14341 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14401 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14461 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14521 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14581 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14641 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14701 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14761 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    14821 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    14881 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    14941 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15001 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15061 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15121 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15181 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15241 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15301 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15361 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15421 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15481 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15541 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15601 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15661 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15721 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15781 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    15841 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    15901 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    15961 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16021 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16081 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16141 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16201 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16261 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16321 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16381 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16441 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16501 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16561 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16621 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16681 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16741 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16801 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    16861 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    16921 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    16981 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17041 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17101 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17161 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17221 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17281 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17341 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17401 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17461 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17521 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17581 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17641 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17701 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17761 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    17821 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    17881 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    17941 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18001 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18061 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18121 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18181 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18241 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18301 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18361 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18421 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18481 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18541 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18601 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18661 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18721 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18781 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    18841 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    18901 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    18961 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19021 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19081 acatttgtgt tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta
    19141 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19201 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19261 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19321 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19381 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19441 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19501 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19561 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19621 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19681 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19741 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19801 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    19861 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    19921 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    19981 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20041 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20101 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20161 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20221 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20281 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20341 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20401 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20461 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20521 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20581 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20641 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20701 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20761 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    20821 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    20881 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    20941 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21001 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21061 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21121 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21181 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21241 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21301 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21361 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21421 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21481 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21541 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21601 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21661 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21721 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21781 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    21841 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    21901 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    21961 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22021 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22081 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22141 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22201 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22261 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22321 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22381 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22441 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22501 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22561 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22621 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22681 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22741 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22801 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    22861 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    22921 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    22981 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23041 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23101 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23161 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23221 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23281 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23341 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23401 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23461 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23521 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23581 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23641 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23701 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23761 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    23821 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    23881 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    23941 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24001 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24061 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24121 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24181 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24241 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24301 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24361 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24421 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24481 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24541 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24601 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24661 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24721 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24781 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    24841 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    24901 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    24961 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25021 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25081 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25141 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25201 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25261 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25321 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25381 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25441 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25501 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25561 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25621 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25681 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25741 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25801 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    25861 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    25921 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    25981 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26041 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26101 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26161 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26221 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26281 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26341 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26401 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26461 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26521 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26581 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26641 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26701 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26761 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    26821 ttattttata cattttatta gctttgatta tcccatttgc gcgttctgca cgaacaaact
    26881 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    26941 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27001 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27061 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27121 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27181 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27241 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27301 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27361 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27421 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27481 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27541 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27601 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27661 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27721 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27781 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaataaa aatgggatag
    27841 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    27901 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    27961 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28021 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28081 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28141 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28201 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28261 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28321 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28381 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28441 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28501 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28561 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28621 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28681 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28741 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28801 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    28861 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    28921 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    28981 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29041 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29101 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29161 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29221 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29281 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29341 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29401 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29461 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29521 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29581 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29641 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29701 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29761 atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    29821 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    29881 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    29941 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30001 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30061 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30121 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30181 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30241 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30301 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30361 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30421 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30481 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30541 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30601 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30661 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30721 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30781 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    30841 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    30901 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    30961 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31021 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31081 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31141 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31201 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31261 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31321 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31381 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31441 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31501 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31561 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31621 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31681 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31741 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31801 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    31861 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    31921 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    31981 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32041 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32101 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32161 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32221 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32281 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32341 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32401 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32461 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32521 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32581 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32641 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32701 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32761 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    32821 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    32881 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    32941 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33001 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33061 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33121 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33181 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33241 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33301 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33361 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33421 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33481 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33541 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33601 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33661 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33721 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33781 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    33841 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    33901 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    33961 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34021 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34081 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34141 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34201 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34261 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34321 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34381 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34441 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34501 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34561 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34621 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34681 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34741 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34801 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    34861 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    34921 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    34981 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35041 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35101 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35161 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35221 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35281 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35341 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35401 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35461 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35521 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35581 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35641 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35701 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35761 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    35821 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    35881 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    35941 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36001 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36061 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36121 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36181 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36241 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36301 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36361 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36421 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36481 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36541 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36601 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36661 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36721 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36781 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    36841 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag
    36901 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    36961 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37021 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37081 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37141 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37201 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37261 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37321 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37381 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37441 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37501 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37561 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37621 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37681 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37741 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37801 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    37861 aaaatgctgt taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc
    37921 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    37981 ggaaaagcgt acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc
    38041 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38101 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38161 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38221 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38281 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa
    38341 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38401 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38461 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38521 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38581 atttcgaaaa tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca
    38641 tatggaaaag aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag
    38701 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38761 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    38821 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa
    38881 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    38941 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39001 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39061 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39121 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39181 gcttattttt agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt
    39241 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt
    39301 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39361 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39421 gtttactagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39481 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39541 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39601 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39661 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga
    39721 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39781 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    39841 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    39901 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    39961 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40021 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40081 accaagaatt agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc
    40141 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40201 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40261 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40321 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40381 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40441 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40501 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40561 gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca
    40621 ctggcgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40681 aattaaatct gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40741 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg
    40801 tttcctgcaa acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    40861 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    40921 gagcagaagt ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt
    40981 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41041 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa
    41101 acggcaacgc aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat
    41161 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41221 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt
    41281 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41341 gtgctacagc aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat
    41401 tgctcatagc tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga
    41461 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41521 ccatcgacaa ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt
    41581 cttcgtcttg gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat
    41641 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41701 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41761 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    41821 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    41881 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    41941 cgattgctgc accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga
    42001 tccataaaat gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca
    42061 tttgggtcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42121 acatcaaaat aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg
    42181 accagttacc tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa
    42241 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42301 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat
    42361 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42421 aaaaattaaa gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat
    42481 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42541 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42601 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42661 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42721 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atgggacaaa
    42781 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    42841 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tattttgtaa
    42901 tttgttagac tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg
    42961 ataaagacaa aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca
    43021 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43081 agttcctcca aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt
    43141 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gctttcttac aatatctttt
    43201 tcgttatatc tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg
    43261 gcagcagtta ttcaaacatt ttacaaaaat ggacaatctc ttttaccaaa aatatcggac
    43321 tggggatttg atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43381 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgtgatgat
    43441 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43501 cacttcacga gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43561 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43621 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43681 aaacaaacga gtcaattttc tagactcctt atttgatcca ttcattacct tgattatcgg
    43741 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43801 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    43861 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    43921 acttttgcat gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggtaaa
    43981 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44041 agaacaaatc aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44101 tgcgggaaaa acaacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44161 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44221 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44281 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44341 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44401 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44461 attattaata ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt
    44521 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44581 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44641 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44701 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44761 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    44821 aaacctttta gaacaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc
    44881 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    44941 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt cctagtaaaa
    45001 agtatcattt ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag
    45061 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45121 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45181 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc
    45241 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga
    45301 attgtcatct ggtattacca gaaatttagt tctcgtattt accgaaatat gcgggaaaaa
    45361 ttaagtcagc tgaatacaaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45421 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45481 acgcgctatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45541 tatgcattgg cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc
    45601 gtagaagttg gactgatcta tgcatttacg acttatgtcc agggattttt taatccaatg
    45661 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45721 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45781 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    45841 gtattaaaca atatctcttt tgttgctaat ccgggagaaa ccgttgcatt agtgggacac
    45901 accggaagtg ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa
    45961 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46021 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46081 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46141 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46201 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46261 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46321 atccaagaag gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg
    46381 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46441 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46501 caaagtagtg gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg
    46561 aaaataaaac atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga
    46621 tttaaaaaag acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac
    46681 aaaatcgttt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46741 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46801 ttgttttgga tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt
    46861 actgaataac aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg
    46921 aaaaggattg gcattcaaaa agaaggtggg tgacccgatt cttgactcat gcatcgacaa
    46981 aaaatttcag ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat
    47041 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca
    47101 agtccattta aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga
    47161 acgtcaaaaa atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt
    47221 tcctaaagag tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47281 caagttatca gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47341 tcaaagccag caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat
    47401 cgtccgctat tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt
    47461 cacacacctg cgtttttttc tgacacgtgt cctagatgga gcgaaatcag aagatggaga
    47521 agtttctgag gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt
    47581 tgagaaaata gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa
    47641 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa
    47701 aaaggaggac aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa
    47761 ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac
    47821 atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga
    47881 cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa
    47941 gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg
    48001 ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagc agaagaagag
    48061 gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag
    48121 ccttttttag gtgctttggc tgcagcggga atggtcaaag gattaaatgc tttgttcttg
    48181 tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat
    48241 gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg
    48301 aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca
    48361 gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg
    48421 gcttatgaaa catttttggg tatcccatgg gtgggcagca gttatactag tagcgtcgta
    48481 cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt
    48541 cctgcagtcg tccagacctt tttggtttct ttttctgtgt tattaatcgc acttcctgct
    48601 ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc
    48661 caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa
    48721 gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg
    48781 caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca
    48841 gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca
    48901 gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg
    48961 aagaagaagc catttattta ctcgatgatt ggtggtgctg tttcaggaat ctatttgatg
    49021 gcatctggaa caatcagcta cataatgggc ggattaggga tttttggggt ggtaaactac
    49081 atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg
    49141 gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct
    49201 atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca
    49261 ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttaatc
    49321 taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagttatgac cctgttcccg
    49381 acgaaacatg caatcggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga
    49441 ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga
    49501 gtgagacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac
    49561 agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac
    49621 caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg
    49681 tgaagaatgg actaggctta actattcttt ttattccact tctttatttt caaaattgaa
    49741 aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat
    49801 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
    49861 aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg
    49921 tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt
    49981 caaagctaaa attggaacat cattttgttt caatatttct gcggtacgaa ggatcacatt
    50041 gttttcaccc gtataagaaa tcagaatccc gcacatatcg ctcgtacaat tgtacgcttc
    50101 atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt
    50161 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt
    50221 tgcctggagc agtaaatatt tagctttttg cagttggtca tgatgaatga gagaaagtgt
    50281 atcatgaatc gtcatctgtt ctaaagaagc cattttattt gcgattgtca taataccgtc
    50341 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
    50401 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
    50461 acggatgagt gtagacgggt ggacataaca tcgctcagct atcgcttgga tggtcatttc
    50521 gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa
    50581 ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac
    50641 gtaaattata acttttttga aaatgttttg taaacggatt tatactatat aacttaaaat
    50701 aggctttttt tgacaagata tttacaaaaa tccactagaa aacagtaact tttgtaaatg
    50761 ttactggaaa gaaacggatt tctattgttt gttttgttta caaaaaagat aatgaaagtg
    50821 taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg attggtggag
    50881 gcagcagtta cacccctgag ttgatggaag gatttatcaa aagatatgag gaattaccga
    50941 tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag atcgttagtc
    51001 agatgtctca gcgtatgtgg gatgcttctc catatgaagt caagatctat gcaactctgg
    51061 atcgggaaga agcattgaaa gatgcggatt ttgttacgac tcagtttcgt gtcggtcaat
    51121 tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt gggcaagaaa
    51181 caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt ttggcaatcg
    51241 ttgaggacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg aacccaagtg
    51301 ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt ggattatgca
    51361 atgtgccagt gatggcgatg atgacagaac cagcaatgct tggcaagaaa aaagaagagc
    51421 tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca gataataaag
    51481 gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagataat ggattaccga
    51541 aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaac atgatccctt
    51601 gtggctacca ccgctattac tatcgtgaag aagaaatgct ggaacatgct ttagaagaat
    51661 atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag ctatttgaat
    51721 tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga ggtggcgctt
    51781 attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa gaaacacaga
    51841 tcgttgtatc tacaaagaat gatggagctg ttcctgacct gtcggcagat tgtgttgtag
    51901 aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt tctcttcctg
    51961 ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg atcgaagcag
    52021 ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatcca cttatacgat
    52081 caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag cagtatttac
    52141 cgcaattcaa ggaaacaatc agccgattaa aagaagaagg aatcgaagtt caagatgaac
    52201 tggcaagaga attatcaggt aatacacata taatggagga agtataatgg ggttcagaaa
    52261 agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcgctt atgatgaagg
    52321 tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt ttcctgttat
    52381 caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag caaaaattgc
    52441 aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa tggggttcaa
    52501 gacttatcga atgtctatcg cctggagtcg tatttttcca atgggggatg agaaagagcc
    52561 gaatgaagct ggcttgaaat tttatgagga tgtctttaaa gaatgccaaa agcacggaat
    52621 tgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca aagaatatgg
    52681 tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag tcatcttcaa
    52741 tcgctataaa ggacttgtca aatattggct gacattcaat gagatcaata tgatccttca
    52801 tgcaccattt atgggcgcag ggctgtattt tgaagaagga gaaaatgaag aacaaatcaa
    52861 atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga ttgctcatga
    52921 agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt atccatacac
    52981 ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt atttcttcat
    53041 tgatgtccag tcacgaggag aatatcccgc ttatgcattg aaagaaatgg agcgaaatgg
    53101 cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata ctgttgattt
    53161 tatctctttt tcttattatt cttcacgagt ttcgactacc gacaacgaac tcttggaaca
    53221 gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa gtgaatgggg
    53281 gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg atcgttatca
    53341 aaaaccatta tttattgtag aaaacggctt aggtgcagtc gatacgccag atgaaaatgg
    53401 ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg cgatgaagga
    53461 tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat gtatcgattt
    53521 agtatcagct ggtaccggtg agatgaagaa aaggtacgga ttcatttatg tcgatcgtga
    53581 taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact ggtataaaaa
    53641 agtaattgat tcaaatggag aaaatctttc gaacaattga tcaatgattt ataaaagtct
    53701 attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc aagcagaaca
    53761 atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa aggagaatct
    53821 acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg ctgctttgta
    53881 tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg gcggccaaat
    53941 gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg gccccgatct
    54001 agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt atggaatcgt
    54061 tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata aaagttacga
    54121 aggaaaaaca gtcattatcg caacaggctg tatccaccgt aaattaggcg tacctggcga
    54181 ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg cgtttttccg
    54241 taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag ccatctattt
    54301 gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac gtgcacaaaa
    54361 gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg atacagtcgt
    54421 tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta atgtcaaaac
    54481 cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac tggatccatt
    54541 aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc caacagacaa
    54601 cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg aaaaagattt
    54661 gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag tgtataaata
    54721 cttggagtct ttagaagaaa caacagcata agtttaaaaa gagtgaaaga acggtttcaa
    54781 ttgttctttt actctttttt ttcgcgtgaa atccactgaa tccgatttat gtgaatactt
    54841 gcaatcatgc ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt
    54901 tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat
    54961 tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa
    55021 cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta
    55081 tgcaccgttg gaattcggta ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg
    55141 aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca tggatacaca
    55201 aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc atcagtcacc
    55261 agaatttgca atggaagcag ccaaaacact ggcacatcac aatatcccgt cttatgtatt
    55321 cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga agacgtttgc
    55381 gggaatcatg gtaaccgcat ctcacaaccc agcggcttac aatggatata aagtctacgg
    55441 agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga
    55501 agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac atagcgggtt
    55561 gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca aagcagtgac
    55621 gattgatcgt gaattggttg aaacaatggg gaaagatttg aaacttgtgt acacgccact
    55681 tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt
    55741 cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca aatcgccaaa
    55801 tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag agggagctga
    55861 cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc gtttgccaga
    55921 tggttcttat gaagtattga ctgggaacca aatcggagca ttgtttgctc aatatatttt
    55981 ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga aatcaatcgt
    56041 ttccagcgaa ttgcctacag caattgcgga acactacggt gctactatgt tcaatgtgtt
    56101 gacagggttt aaattcattg ctgaaaaaat ccaacaattt gaagaagacc attcacatac
    56161 ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg ttcgcgataa
    56221 agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata aaaaacaagg
    56281 caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt tcgcagaaaa
    56341 aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga
    56401 aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc aaacagaaga
    56461 ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa caacaccgcc
    56521 atccgacgta ttgaagtata cattggaaga aaatggctgg atcgctatcc gcccttcagg
    56581 aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag aagacgcagc
    56641 cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat aaaaaacttg
    56701 agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt ggaggtgaat
    56761 ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt caaaatgttg
    56821 agtgacccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca aaatgtgaca
    56881 gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact acgtttatta
    56941 agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta tagtcttgat
    57001 gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca tcaataacga
    57061 taggtgcaaa tagataaaca ctggagtgtg acaacgaaat ttcttcgttg tcacactcca
    57121 gtgtttttta agaaaaagac aaaaatgatt aatcgctttt ttctgttgtt tcagttccat
    57181 aataactatg atcttctagg tctaaagcat tcaaaataag cgaagccgtt tcttcaatag
    57241 ataaagatgc cacattgatc actacacatc ctagtttttc ataaagatca ttcgcaaaat
    57301 caagttcagc tctaattttt tcgatatcgg aataagcagt atcagggttt aatccgtaag
    57361 agcgcatccg ttctttacgg atattattta aaatatctgg gttattcgtt agtcccacga
    57421 tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta
    57481 aattggcaac ttttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc
    57541 gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc acgaggatct ttcccatcat
    57601 cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat
    57661 ggagggcacc aggttgccga attggcttga taccagttaa acgttcaatt tctgatatcg
    57721 gtggcgtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgtgttatct
    57781 cgatcagttc ttgattgatt aatgtatgca ggacaagtgc tttttcttct aatgcatctt
    57841 gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt tctgcggaat aggttgaact
    57901 caacggtagg atactgagcc atagtagctt gcgctagttt tgtcgcagtt tctccagcag
    57961 agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg gataaaacct
    58021 cacttgcatt ttttttatgt ttattatacc atactctaga atgcgtactt cgtaatcatt
    58081 ttgatttgga ggaataagtc atgaaaaaac agacattgat cgaacaatca ttagaaacat
    58141 tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga
    58201 agcgtaaccg ctacgtatct gctaaagaag tttttgacta tatgaatcag gaattcaaag
    58261 gggtaagtta tgatacgatt taccggaatc tccatgattt tgcagagatc gatttactgg
    58321 aagaaaccga tttaaatgga gaaatgaagt ttcgttttca ttgctgtcag gggaaaatcg
    58381 gccatcacca tcattttatc tgtacagtgt gcggaacaac gaaagaaatc catatgtgtc
    58441 cgatgaatta tttcgaagaa cagctaggtg gatgcacgat cgaagggcat agattcgaga
    58501 ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt
    58561 aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca
    58621 gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga
    58681 gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg
    58741 tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt
    58801 ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa
    58861 attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat
    58921 gacatgaaag cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc
    58981 aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa
    59041 ctaacggtgt tgtctcgtga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa
    59101 gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtaatagt cgaaaaatat
    59161 atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcactcaa
    59221 actggtgctt catcaatgaa agaatttggc aaagttatgg gtgcaattat gcctaaagtc
    59281 aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga aagaactatt acaatcttaa
    59341 ttttatcatt taaactgcgg acagtgatta tccgcagttt tttgtgttcg taaaaaaaga
    59401 actaaaaaac agctagaaga aa
//
LOCUS       DAYYCM010000010        56174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000010 DAYYCM010000000
VERSION     DAYYCM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.010"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            223..1167
                     /gene="mvk"
                     /locus_tag="ACHAY1_000707"
     CDS             223..1167
                     /gene="mvk"
                     /locus_tag="ACHAY1_000707"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8592727.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1160..2137
                     /gene="mvaD"
                     /locus_tag="ACHAY1_000708"
     CDS             1160..2137
                     /gene="mvaD"
                     /locus_tag="ACHAY1_000708"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8592728.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2150..3235
                     /locus_tag="ACHAY1_000709"
     CDS             2150..3235
                     /locus_tag="ACHAY1_000709"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8592729.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3232..4296
                     /gene="fni"
                     /locus_tag="ACHAY1_000710"
     CDS             3232..4296
                     /gene="fni"
                     /locus_tag="ACHAY1_000710"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8592730.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4545..5348)
                     /locus_tag="ACHAY1_000711"
     CDS             complement(4545..5348)
                     /locus_tag="ACHAY1_000711"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8592731.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5368..6210)
                     /gene="kduI"
                     /locus_tag="ACHAY1_000712"
     CDS             complement(5368..6210)
                     /gene="kduI"
                     /locus_tag="ACHAY1_000712"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8592732.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6226..7233)
                     /locus_tag="ACHAY1_000713"
     CDS             complement(6226..7233)
                     /locus_tag="ACHAY1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8592733.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7240..7884)
                     /locus_tag="ACHAY1_000714"
     CDS             complement(7240..7884)
                     /locus_tag="ACHAY1_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8592734.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7984..8742)
                     /locus_tag="ACHAY1_000715"
     CDS             complement(7984..8742)
                     /locus_tag="ACHAY1_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8592735.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8869..10020)
                     /gene="fucO"
                     /locus_tag="ACHAY1_000716"
     CDS             complement(8869..10020)
                     /gene="fucO"
                     /locus_tag="ACHAY1_000716"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8592736.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10102..10413)
                     /gene="rhaM"
                     /locus_tag="ACHAY1_000717"
     CDS             complement(10102..10413)
                     /gene="rhaM"
                     /locus_tag="ACHAY1_000717"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8592737.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10415..11260)
                     /gene="rhaD"
                     /locus_tag="ACHAY1_000718"
     CDS             complement(10415..11260)
                     /gene="rhaD"
                     /locus_tag="ACHAY1_000718"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8592738.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11274..12530)
                     /gene="rhaA"
                     /locus_tag="ACHAY1_000719"
     CDS             complement(11274..12530)
                     /gene="rhaA"
                     /locus_tag="ACHAY1_000719"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8592739.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12527..13987)
                     /gene="rhaB"
                     /locus_tag="ACHAY1_000720"
     CDS             complement(12527..13987)
                     /gene="rhaB"
                     /locus_tag="ACHAY1_000720"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8592740.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14140..15009
                     /locus_tag="ACHAY1_000721"
     CDS             14140..15009
                     /locus_tag="ACHAY1_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8592741.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15135..16034)
                     /locus_tag="ACHAY1_000722"
     CDS             complement(15135..16034)
                     /locus_tag="ACHAY1_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592742.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16199..17023)
                     /locus_tag="ACHAY1_000723"
     CDS             complement(16199..17023)
                     /locus_tag="ACHAY1_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8592743.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(17034..18329)
                     /locus_tag="ACHAY1_000724"
     CDS             complement(17034..18329)
                     /locus_tag="ACHAY1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8592744.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18345..18824)
                     /locus_tag="ACHAY1_000725"
     CDS             complement(18345..18824)
                     /locus_tag="ACHAY1_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8592745.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18830..19858)
                     /locus_tag="ACHAY1_000726"
     CDS             complement(18830..19858)
                     /locus_tag="ACHAY1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8592746.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19875..20981)
                     /locus_tag="ACHAY1_000727"
     CDS             complement(19875..20981)
                     /locus_tag="ACHAY1_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8592747.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21210..23807)
                     /gene="adhE"
                     /locus_tag="ACHAY1_000728"
                     /gene_synonym="adhC"
     CDS             complement(21210..23807)
                     /gene="adhE"
                     /locus_tag="ACHAY1_000728"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8592748.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(24112..24492)
                     /gene="yajC"
                     /locus_tag="ACHAY1_000729"
     CDS             complement(24112..24492)
                     /gene="yajC"
                     /locus_tag="ACHAY1_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8592749.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(24589..25734)
                     /gene="tgt"
                     /locus_tag="ACHAY1_000730"
     CDS             complement(24589..25734)
                     /gene="tgt"
                     /locus_tag="ACHAY1_000730"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8592750.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            25952..26650
                     /locus_tag="ACHAY1_000731"
     CDS             25952..26650
                     /locus_tag="ACHAY1_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8592751.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(26818..29457)
                     /locus_tag="ACHAY1_000732"
     CDS             complement(26818..29457)
                     /locus_tag="ACHAY1_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8592752.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(30076..31107)
                     /gene="queA"
                     /locus_tag="ACHAY1_000733"
     CDS             complement(30076..31107)
                     /gene="queA"
                     /locus_tag="ACHAY1_000733"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8592753.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            31300..32475
                     /locus_tag="ACHAY1_000734"
     CDS             31300..32475
                     /locus_tag="ACHAY1_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8592754.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            32479..33120
                     /locus_tag="ACHAY1_000735"
     CDS             32479..33120
                     /locus_tag="ACHAY1_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592755.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            33117..34034
                     /locus_tag="ACHAY1_000736"
     CDS             33117..34034
                     /locus_tag="ACHAY1_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8592756.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            34031..34702
                     /locus_tag="ACHAY1_000737"
     CDS             34031..34702
                     /locus_tag="ACHAY1_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592757.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(35243..35731)
                     /locus_tag="ACHAY1_000738"
     CDS             complement(35243..35731)
                     /locus_tag="ACHAY1_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8592758.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(35932..36198)
                     /locus_tag="ACHAY1_000739"
     CDS             complement(35932..36198)
                     /locus_tag="ACHAY1_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8592759.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(36198..36461)
                     /locus_tag="ACHAY1_000740"
     CDS             complement(36198..36461)
                     /locus_tag="ACHAY1_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8592760.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            36982..37122
                     /locus_tag="ACHAY1_000741"
                     /pseudo
     CDS             36982..37122
                     /locus_tag="ACHAY1_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(37264..39087)
                     /locus_tag="ACHAY1_000742"
     CDS             complement(37264..39087)
                     /locus_tag="ACHAY1_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8592761.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(39685..42390)
                     /locus_tag="ACHAY1_000743"
     CDS             complement(39685..42390)
                     /locus_tag="ACHAY1_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8592762.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(42696..43643)
                     /locus_tag="ACHAY1_000744"
     CDS             complement(42696..43643)
                     /locus_tag="ACHAY1_000744"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8592763.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            43833..44102
                     /gene="rpsN"
                     /locus_tag="ACHAY1_000745"
     CDS             43833..44102
                     /gene="rpsN"
                     /locus_tag="ACHAY1_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8592764.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(44278..44640)
                     /locus_tag="ACHAY1_000746"
     CDS             complement(44278..44640)
                     /locus_tag="ACHAY1_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8592765.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(44651..45772)
                     /gene="alr"
                     /locus_tag="ACHAY1_000747"
     CDS             complement(44651..45772)
                     /gene="alr"
                     /locus_tag="ACHAY1_000747"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8592766.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(45788..46138)
                     /gene="acpS"
                     /locus_tag="ACHAY1_000748"
     CDS             complement(45788..46138)
                     /gene="acpS"
                     /locus_tag="ACHAY1_000748"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8592767.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(46268..47779)
                     /locus_tag="ACHAY1_000749"
     CDS             complement(46268..47779)
                     /locus_tag="ACHAY1_000749"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8592768.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(48101..49453)
                     /gene="murF"
                     /locus_tag="ACHAY1_000750"
     CDS             complement(48101..49453)
                     /gene="murF"
                     /locus_tag="ACHAY1_000750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8592769.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(49606..50682)
                     /locus_tag="ACHAY1_000751"
     CDS             complement(49606..50682)
                     /locus_tag="ACHAY1_000751"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8592770.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            50878..52623
                     /locus_tag="ACHAY1_000752"
     CDS             50878..52623
                     /locus_tag="ACHAY1_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8592771.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(52903..53340)
                     /locus_tag="ACHAY1_000753"
     CDS             complement(52903..53340)
                     /locus_tag="ACHAY1_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8592772.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(53324..54697)
                     /locus_tag="ACHAY1_000754"
     CDS             complement(53324..54697)
                     /locus_tag="ACHAY1_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592773.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(55019..55507)
                     /locus_tag="ACHAY1_000755"
     CDS             complement(55019..55507)
                     /locus_tag="ACHAY1_000755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592774.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(55529..56131)
                     /locus_tag="ACHAY1_000756"
     CDS             complement(55529..56131)
                     /locus_tag="ACHAY1_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8592775.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        15979 a        12182 c        10285 g        17728 t
ORIGIN      
        1 tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa aaaagtgttt tttctttaga
       61 aaacgtattc ttagttttac ttctgagatg gttttagtaa aataaaggag gaatgttttt
      121 tgagaccctt agtggacaaa aaaattcgtg aagcagcgca actcgttttg ggcgctttat
      181 tttttgctta aaaatcactt ttacgtgaga aggagttaga ttatggcaaa ctatggccaa
      241 ggagaatcaa gcggaaagat catattgatg ggcgagcacg cggttgttta tggagaacca
      301 gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat tccttgaaga gctggatgca
      361 atggacgatc aactggtttc ttcctactat tcaggaaatt tagccgaagc tcctcatgca
      421 ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac agcatgacat ccaaaaaaac
      481 ttgcaactga ccattgaaag cacgattcct gctgaacgtg gaatgggatc aagcgctgca
      541 gtcgccacag cagtcactcg tgctttttat gattacttag catttccttt gtctcgtgaa
      601 atactattag aaaatgtcca gctttcggaa aaaatcgccc atggtaatcc tagtggaatc
      661 gatgcagccg ctactagcag cttgcagccg atttatttta caaaagggca tcctttcgac
      721 tacttttctt tgaacatcga tgcttttttg attgtcgctg atacaggaat caaaggacaa
      781 acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa cccagcctca tgaaactgga
      841 caaatgatcc aaaaattagg atacttgacg aagcaagcaa aacaagcaat cattgaaaat
      901 tcaccagaaa cgttggcaca gacaatggat gaatcacaat cacttctgga aaagctgaca
      961 atcagcaatg attttcttaa tctattgatc caaacagcaa aagataccgg ggcattgggt
     1021 gccaaattaa ctggcggtgg acgcggtggc tgtatgattg cattagcaca aacaaaaaca
     1081 aaagcccaag aaatcagtca agcacttgaa gatgcgggtg ctgctgaaac ttggatacaa
     1141 ggattaggag tacataccta tgtttaaagg caaagcacgc gcatatacca acattgctct
     1201 aatcaaatat tggggtaaga aaaatgaaga actcatcctt ccaatgaaca acagcctttc
     1261 gttaacattg gatgcatttt atacagagac agaagtcatc ttttctgata gctatatggt
     1321 agatgaattt tatctagatg gcaccttgca agacgaaaaa gcaacaaaaa aagtcagtca
     1381 gtttcttgac ctttttcgta aagaagccgg cctctcacta aaagcttcag taatcagtca
     1441 gaatttcgtt cctaccgcag ctggattagc ctcctctgcc agcgggctag ctgctttagc
     1501 aggcgcttgc aatactgctc ttaagcttgg attagatgat ctctctcttt caagatttgc
     1561 tcgacgcggg tctggttcag cttgccgaag tattttcggt ggtttcgtcg aatgggaaaa
     1621 aggccatgac gacttaagtt cttacgctaa gccagtccct tccgattctt tcgaagacga
     1681 tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa gaagtgtcca gcagaaatgg
     1741 gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc tggttagatt ccgttgaagg
     1801 ggatctatat caattgaaac aagcaatcaa aacaaaagat ttccaacttc tcggagaaac
     1861 gatggaaaga aacggactaa aaatgcatgg aacgacttta gctgcccagc ctccattcac
     1921 ttactggtct cctgacagct taaaagcgat ggatgcggtg cgacaattga gaaagcaagg
     1981 cattccatgc tactttacga tggatgctgg ccctaatgtc aaagttttag tggaaaacag
     2041 ccatttatct gaggtacaag aaacatttac caaattattt agcaaagaac aagtaattac
     2101 tgcacatgct ggtccaggaa ttgcgattat tgaataaagg gagattttta tgattgaagt
     2161 atctgcacca ggcaaactct atattgccgg agaatatgca gttgttgaaa caggccatcc
     2221 agcagttatc gctgcagtcg atcaattcgt gacagtcact gtagaatctg cacgaaaagt
     2281 cggaagtatc caatctgcac aatatagtgg gatgcctgta cgttggacaa gacgcaacgg
     2341 agaattagtt ttagatattc gggaaaatcc ttttcattat atccttgctg ctattcgctt
     2401 gactgaaaag tatgcacaag aaaaaaacat tcttttatcc ttttatgatc tgaaagtaac
     2461 aagtgagtta gacagttcaa atggccggaa atatggattg ggttctagcg gtgccgtgac
     2521 cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg aatttatctc agttggagat
     2581 tttcaagatt gcagcactag ccaatttagc agttcaagat aatggttcct gcggcgacat
     2641 cgctgctagc tgttatggtg gctggatcgc tttctcaacc ttcgaccatc cttggctcca
     2701 agaacaagaa actcagcatt ctatcagtga gttacttgcc ctggattggc caggtctatc
     2761 cattgagcca ttgattgctc ctgaagattt acgtttattg attggttgga cgggtagccc
     2821 tgcctctact tctgatttgg tcgatcaagt tcaccgttcg agagaagata aaatggtggc
     2881 ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac gaaatgatca aagggtttaa
     2941 agaaaataat gtaacgttga tccaacagat gattcgaaaa aaccgacaat tactgcatga
     3001 tttatctgca atcactgggg tcgtcatcga aacgcctgct ttgaacaaat tgtgtaattt
     3061 agctgaacag tatgaaggag ccgcaaaatc ttctggtgca ggtgggggcg attgcggaat
     3121 cgtaattgtt gaccagaaat ctggcattct tcctttaatg agtgcatggg aaaaagcaga
     3181 aatcactcca ctgccgttac atgtctatag cgatcaaaga aaggaaaacc gatgaatcga
     3241 aaagacgaac atgtttcact agctaaagcc tttcatgata aacaaaaaaa cgaatttgac
     3301 ttcgtacgga tcatccataa ccctttgccg caaatagcag tttctgatgt cgatctaagt
     3361 acacaggcag ttgggtttac actaagcagc ccattttata tcaatgcgat gactggcgga
     3421 agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg tcgcaagaga agcagatttg
     3481 atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc cttctttagc cgatacttat
     3541 acgattatgc gacaagaata tcctcacggg aaaatcattg ccaacatcgg tgcagggact
     3601 tctgtcgaaa gagccaaaga agctatccga ttgtttcacg cagatgcctt acagattcat
     3661 ttgaatgcac ctcaagagtt ggttatgcct gagggagacc gtgattttac caattggaaa
     3721 gtacttattc aagaaactca aacagccatt gacgtccccc tcatcgtaaa agaagtcggt
     3781 tttggcatga caagagaaac gctcaatgat ctagctgctt taggagttca tacagttgat
     3841 atcagcggtc gaagcggaac aagcttcacc cagatcgaaa acgcccgtcg ctccaagcgg
     3901 gaactgagtt atttagctga ctgggggcaa tcgacagtct cttcattgct tgaagcaaat
     3961 gaagcagaca cttctatgga aatcctagcc tccggaggta tccgcaatgc ctatgacata
     4021 tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt caggaaccgt actgacgcat
     4081 ctgatgaatc acggcgtaga agaaactatt atactgatga agcaatggca agaagagctt
     4141 cgtctgcttt atactatggt cggcgcaacg aataccgcgg ccctacacca acaatctctg
     4201 atcttttccg gaccagtgaa ggattggtgc gaagcaagag ggatcgactt agtgaagtat
     4261 ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     4321 aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga aataagctga aattcacaaa
     4381 aatttgaaaa gcaattttcg tgaattcctt cttatttctt ggagttaaat gcttctgtcc
     4441 cagcctcttt tttcgaataa atgatgttta atcagctgtc cttcggtatt cgttcaatcc
     4501 gctgatttca caacctctcc actaaacagt gtactaaagc tcttttaagg ttgttttccg
     4561 atataggcaa gtatgcctcc gtccacatac aagacatggc cgttaacgaa atcagatgcg
     4621 ctagaagcca agaagacaga tggtccagcc aaatcgattg gctcgcccca gcgtgctgct
     4681 ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt caccgttttc ttgtgtttcg
     4741 cgtaatggag cagtttgcgg tgtagcaata taacctggtc caattccatt acattggata
     4801 ttgtattgac cgtattcaga agcgatgttt ttcgtcagca tttttaatcc gccttttgcc
     4861 gcagcataag cactgaccgt ttcgcgtcct aattcactca tcatggagca gatattgatg
     4921 attttaccgc cgcctttttc gatcatatcc ggaataactg cttttgaaac gatgaatggg
     4981 gcatttaaat ccacgtcgat tacttggcgg aagtctgccg catccatttc gatcatcggt
     5041 gtccgtttga tgatccctgc attgttgact agaatatcaa ctgaacctac atcttctttg
     5101 atctgttgaa ccatcgcttg aaccgcttct tcatcggtca catcgcagac ataacctttt
     5161 gcatcgatgc ctgcttcttt ataatgcgct ataccttcat caactgattc ttgattcaag
     5221 ttattaaata cgatttttgc tcctgctgta gctaaagagc gtgcaatctc aaatccaata
     5281 ccatagacag caccggtgac taatgctact tttccatcta aacgaaacat atccatacta
     5341 aattccatca ttttctctcc cttatgctta ttttaattga tccatttgca ccatatccat
     5401 gtctgtatag gtgatatttt ctccgcacat tgcccagatg aatgagtaat tgcttgtacc
     5461 tacacctgaa tggatcgacc agcttggcga aataatggct tgttcattgt ctacaactaa
     5521 atgttttgtt tcatctggtt tgcccatcat gtggaacaca cgtgtatctt cattggcata
     5581 atcaaagtag acataagctt ccatacgacg ttcatgtgta tgacaaggca tcgtattcca
     5641 agcgcttcct ggttctaaaa tcgtataacc catttgtaat tgacagcttt cgcatacatt
     5701 tggatggatg tattggtaga ttttacgttc attcagtgtc aggccttcac cagtctccat
     5761 tggttttact tggtcgatac tgatttttac atttggatat ttatgatgag ctggaacaga
     5821 actaatatag aatttagctg ggttttcagc atcttgagaa gaaaatacaa catgtcgtgt
     5881 ttcttttccg atatagtatc cgtcttgttt tttcatttct tctttttgcc catcgatctc
     5941 gatgaatcct ggtccaccga tattgatcac acctagttca cgacgctcta agaagtaatc
     6001 aacacctagt tctttgtcta atgtgatttc taattctttt tcagtaggtg tcactccacc
     6061 aaagatcatt cgatcgttgt gcgtataagt caaactgatt ttccctggtt caaatacctt
     6121 ttctactaaa aattcttttc tcaattgttc cgttgaataa tgacggatat cttctggact
     6181 gtgtgtataa cgtgtttcca tttcttgcat tttcttctac tccttctatc gaatgatttt
     6241 tccagagccg catgctaaaa attgtgcgac ttcttcttga ctaaattggt tgcagtcccc
     6301 atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa tcaatgattt cttgcggaga
     6361 catattggat aaaatcccat ggagcacgcc acctgcaaaa gcatctccgc cacctactcg
     6421 atccacgatt ggggtgattc tatggacttg ggattccaca tattcatggt tcatccacaa
     6481 tgtccccttc aattcatttt cacttgctga atgaacggta cgcttggtcg aataaaacac
     6541 ctgaatattc gggaactttt cctgcatttt ttgataataa taaattagag gttgttcttc
     6601 tgcttctggc gctttctcga tacctaacaa ataaagagca tccatttttc cagctgagca
     6661 gatatctact aaaggtaaaa gctgatggat tacttcgctg gcttcttgct gtgtccataa
     6721 ttttccacga tagttgatat cgaagctgat caggcagcca gctttttttg cagcttctat
     6781 cacctgtttc gtcatttttt gccatttttt agacaaagct ggggtgattc ctgaaacatg
     6841 aaatatatct attccttgga ataactcctc tagcgaccat tccaaatgat ccatttcagc
     6901 aaaacttgat ccggcacgat catagatcac tgaggcggcg cgttcgccac taccagtttc
     6961 catataataa gtacctagtc ttgatccacc agataatacc tgtttcgtat ctactccata
     7021 acattgcaag tgctttttga ctgcttttcc caatgcgtta tcaggtactt ttgtagcaaa
     7081 aatcgcttca tgtccgtaat tagctaaaga aattgctacg tttgcttccc ctccgccata
     7141 atgggcagcg aatcgttcac ttgaatcgat acgggttccc tgctctgtgg acaatcgcaa
     7201 catgatttct ccgagtgtca ctacttttcc cataagcagc tacctcttga tttctttcat
     7261 ttttgctgca tactgttgcg cgacttccgt tactttgtca aaatctcctg tagctgcggg
     7321 tgctaaaagg ttcccaccta ctccgactgt tacgacacca gcatcgaacc actctttcat
     7381 attgtccaga ctcacaccgc cagttggcat gatattgact tgaggcatcg gcgccttgaa
     7441 ggcagaaatg atacttggtc cataagcgct tccaggaaat agtttgacga tatctacccc
     7501 gcttttcagc gctgttttga tctcggtaat agtcatacat cctggtaagt aaggaatttg
     7561 atacagattg cacatctctg ccgtttcttg atcaaaggaa gggctaacaa tatactctgc
     7621 gccagctaag atagccaagc gagctgtgac agcgtctaat actgtgcctg cgccaatcac
     7681 tatttctggt tggtctttgt agaatgacaa aagttctttg attacttgat ctgcttgagg
     7741 gactgtaaaa gtcaactcaa taccagttag accacctttg atgattgcgt ggcaagcatt
     7801 caaagcttct tctttgcttt ttccgcgtac caccgcaatc actccggcgt ttttcaatcg
     7861 agataaaata tcaatttttt tcatcttctc cacctatttc ctatttagaa attttatttt
     7921 ataacttgga aatctatacc catattatac ggcagaaaag aaaaattgca aacgcttttt
     7981 tcattatttt tgaattcttt gctcgatttc gctttttgtt tgcttgaggg cactgattat
     8041 ttgttctttt gtctcatctg ttaaacgata tttcggcata cttacactaa atgcaccaat
     8101 gatttgttcg tttttcacga ttgaggctcc tatacaaaaa atatcacgtt cgatttcttc
     8161 atcgtcaaat gcgatgtggg tttcttttac cgttttgatt tcctttttca accgtaatgc
     8221 attggtgatc gtatattctg tgtaagggat caacggatgc ttttcaagat aatcagtgta
     8281 ctgctcttca ctgaattctg ctaatacggc tttgcccatc gcagaattgt ataatggtcg
     8341 ggtgattccg atttttgaag acatgcggat cgtttgatgt tttggatcta gtttgttcac
     8401 gtacaagatc tcattgttgt ccaatacccc taagtggata gtctcatcga tcttttgctg
     8461 cagctgttct aaaaacggta aagtcaactc taccaaatcg atttgttcaa tgtttttatt
     8521 cgcgtagcga ataagctttg cacctaaacg atagtttttc tctgaatttt tatttacgta
     8581 gccgatcatc aacaaagtat ctagtatttt caagactgta gaagaggtca ttcccacacc
     8641 ctttgcgatc gtctgcaaag agacatcttg gttttccgct aaatagtcta gtattttaga
     8701 ggctttgatc aatacagttc cataaggctt tttattttcc atataatatt ctcctatttc
     8761 tttataagaa aaattatagc ggaaatacca tcaatttaaa agagatatcc agatataaac
     8821 taccatttct ctttacaggc aatttaatgt tgaagatccg cttcgttttt acatcaaact
     8881 ttgatataaa gcaatgattt cttctactgt cgtttctctt ggatttcctg gtgtgcatac
     8941 atctcgcaaa gcatcttctg caattgctgg aatatctgct tcttttacac ctaattcaga
     9001 aatggtttct gggattccaa ccgctttgct caaccgatcg atctcaccac aagccgcttc
     9061 tcttacatct tctaaagaca tttcagctgc tcctttaatc cctagaacaa gagcgatctc
     9121 acggtacttt tctccagtat attctttgtt atatttcatg acagttggca aaagtgctgc
     9181 acacgctacg ccatgaggaa tgttgtacca tgcacttaac gggtgagcca tcccgtgtac
     9241 cagtcctaaa ccgacatttg agaagcccat tcccgcaata tactgtccta atgccatttt
     9301 ttctcgccca ttttgatcgc catctacaga agcttccaaa gaatggccga tgatttcgat
     9361 tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct tttgtgataa acccttcaat
     9421 agcatgcgtc atagcatcca tacctgttga tgcagccaat tttttcggca ttcccatcat
     9481 catatcgctg tcgatgaacg cgacgattgg gatatcatga ggatcaacac aaacaaattt
     9541 tcggtgattt acttcatctg tgatgacgta gttgatcgtt acttctgctg ctgttccaga
     9601 agtcgttggt atagctaata taggtaaaca tggatttttc gtatcagcaa caccttctaa
     9661 actaatgacg tctgaaaact ctggattcgt caaaataatc ccaatggctt ttgccgtatc
     9721 gattggcgaa cctccaccga ttgcgatcaa acagtctgcc tctgcctctt taacaaaagc
     9781 cactccatct tttacatttt ggatcgttgg attaggtacg atcccgtcgt ataaagcata
     9841 agcgattgct gctttatcta gaagttctgt tacttttgtt gctataccgt gctcgatcaa
     9901 tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc tgctgaaatt cagggacgat
     9961 cgactcaatc gctccttttc caaaatacga tgtttcattt aaaatcattc gattactcat
    10021 tttctattcc tcctaatttg tattattttt cacaataaaa acgaaagaac cccaagcaac
    10081 cggttgccca gttgcttgtt gctacagttc aaagactggc atcaattccc aactgactgg
    10141 agaggcatct ggatttgtct ccatgatatc ttccatgtac ttccaccatt tttgattgat
    10201 agatgtattt gccatttttt cccataactt ttcatcttcg atcactaagt aaccaaataa
    10261 caaacttgta ctcggatcaa gatagatcga atagtcgatg accccgtgtt cacgaagtgc
    10321 tgtacgcatt tctggccaaa gttcatcatg tctttttttg tattcttctt gtttttctgg
    10381 gtaaactttc atgcaaaaag cttttttcac catgtcagac acctgctttc gtatgaactt
    10441 cctccaaata acctgctttt ggagtgacac caaacgcatc tgctaaatgc cacaggtttt
    10501 cgtccgtgat tgtttgcaag attggtcctt gcgctttgac gtacgtatag acttctgctg
    10561 ccttttctgc tgtctcgatc aaaccaaaga cttcatccat gtcttttcct gacccgtaga
    10621 ttccatgttg tggccataaa accaatcgag tttctttcat tttttcagct gttgcttccc
    10681 caatctcatt tgttcccgga accatccacg gaatgatgga aattccttct ggaaaaacaa
    10741 ctaagcattc cgtacacatt tgccatagcg tacgactaaa ggaacgttcg tctaattcat
    10801 gcgtaaaact catcgctaat agatgactag catggcaatg cataacgatc cgattatctg
    10861 ggtctacttc taaacgggcg atatgactca tgaaatgact tggcaactcg cttgtaggaa
    10921 ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt gccatcttca gcaactcgaa
    10981 cgattccaag attctcggca ggttcgctcg ctacattttt aaaatatctt cctgaaccag
    11041 ttacaataaa atagcatcct gctaaacggc tggcatcaaa aatcatcgag atgcggcgga
    11101 tcactttttc aggatccaag aacggtgtga tctcttcttc ttttagtaaa taagaaatat
    11161 tcccgccatt tctttcatcc catcccaagc gataaagatt cgacgtcgtt tcgatcattt
    11221 ctttcacata aggcgcttgt aaaatatcta tttttttcaa tatagccaca ctcctatttt
    11281 cttaattcca acacgtcttt ttgatattcc tggacttctt ccagccaatc caaaccaact
    11341 ggaacttgat ttttgtaaca gaaataattc catacatcgg catacgggaa atctttcaat
    11401 tcttccgtat aagccattct tgaagtaaag tcaaaagcca gctccatttc tttcaatcgc
    11461 tcaactggtt ccagcatagc tttcagcagt gctttttgtg tatttctcgt accgatgacc
    11521 caagcagcga tacggttgat cgttgcatca aagaaatcca gaccgatatt tgttttttcc
    11581 aacaattcgt tacggactaa ttctttagcg atttcctgca attcatcatc catcaaaacc
    11641 acatggtctg aatcccaacg cactggacgg gaaacatgca acatgattcc tttgctgaat
    11701 aaagctaatg aagataattt attggaaatc acttctgttg gatgaaaatg tccagcatct
    11761 agacaaacaa gtttgtcacg agtcaacccg taacccatat aaaattcatg ggaaccgact
    11821 gtataagctt cagcgcctaa accaaatagt ttgctttcta ctgcatcttg tacataccgt
    11881 tcatctaact cttctgtaaa aatttcatcg agtgattcca ttaaacgttc ccgtggggta
    11941 aaacgatcaa tcggattatc tttcatccca tcaggtaccc aaaagttatt gatacttgtt
    12001 tgcccagttt ctttcccaaa gtactccgcg attttgcggc ttcttttccc gtgttcgatc
    12061 cagtattcac gaacttcttt atctggatga gccaatgtaa agccgtcttt catcattgga
    12121 tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt ctttttttgc ccactctacc
    12181 catttttcaa aatgtttcgg ctcaatttca tttagatcga cagattcttc tgtatccaaa
    12241 tagatagcat gaaggttcaa tttgtgtttg cccggtatca acgaataagc cttctccaga
    12301 tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa aattccctgt cgccatgatt
    12361 ccaccggtca attcgccatc cggtgtcaag aaacctttga catcatctcc ttgccagcaa
    12421 tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag catctgtatc tacaccgata
    12481 gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa tcgtgttcat acgattactc
    12541 ctttgatttt attcacgtac cattccatcg tgttttgctt attttcatct gggtaaaaag
    12601 tttcacatgg aaacgactgt ttgattacat ttcttgcttg ttctatattc gaaaactgac
    12661 ctgtactgag catttgcatg gcaacattcc cgattgctgt ggcttcacta ggtcctgctt
    12721 ctacaatgat tccggcataa tctgctgtca attgattcag tagacgatta ttcgatccgc
    12781 cccctacgat gtgcagcttt tccaatttcg ttccagttaa gtgttctact ttgatcagct
    12841 cttgtgcgta acaaagagca agattatcat acacacaacg agcgatagct cccgatgaga
    12901 cagggactgg ctgctctgtt tcaaaacagt attgctgaat ctcttctatc atatttgggg
    12961 gattcaaaaa tcgagggtca ttgatttcca tcacttgctg cagcggtttt tcttttgcag
    13021 caagctctgc caattccgca taactgtact cgtactcttg cattcgggca acttcctgga
    13081 tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt gttatgggca ccccattcat
    13141 tcgtataatt ttcagaaaaa gcattgcgag aaacattggc gatctttgtt tcaatcccta
    13201 aaagcgacca tgtcccactg ctgatataac cccaacgttc tccttgacct ggcgttccta
    13261 atacagctga cgccgtatca tgacttgcta ctgtgataaa ggtcacatct ggcaaatcat
    13321 attttgaaaa ttgttctttt tgcagatctc ctagtatcgt tcccggctct gtcaatggtg
    13381 gaaataattc cttcgagaca ccgatttttt caagaagttc tatttcccat tcttttgtcc
    13441 ctgcattcag cagctgcata gtagatgcat tagttttctc cgtcaccatg ttaccagtaa
    13501 agacataccc taaataatca ggtatcagta acaatttatg tgcttttgcc aaattttctt
    13561 tttcttcagc aaacaattgg aacaaggtat tgaaaggctg gatctgaatc cctgtctgct
    13621 catacaactt atctagagaa tagatttcag aaaactgttc tactgctcca ttggtacgct
    13681 tgtcccgata agcaatcgga tcatttatcc gatccccttg ttcatcaatc agacagtaat
    13741 cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat ccctgtctgc tttaattttt
    13801 caaatccaat caaaatctct ttgataagat aatcgatctt ccagcgatcg tgaccgtctt
    13861 tgttccgaaa gccgtttttg aaccggtgta cttcttccaa gatcaaagaa ccttttgcat
    13921 ccctcgtact tttcattagt cgtccgcttg atgcaccgat atcgatggca acaaatgttg
    13981 gattcatttc tccacctctt ttactttatt gttgattagc actttattaa taaagcgtta
    14041 tcatttattg acaatgctat cataatacag aatcttatat tttgcttcac actattttgg
    14101 tatataattg cattatcttg acaagttggg agtgtttaca tggattactg ggaaaaagcc
    14161 aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga cgcctacaca aagaagcagg
    14221 aatttatttt tttatatcca agaaatcggt tacttcaaag caccaatccc ttactatact
    14281 gaacgagcac atttaccttc ttacttgata aagtttactt tgtctggcaa aggttcgctt
    14341 acatataatg ataatcagca acagctaaaa gctggagact tattttttat tgactgccaa
    14401 gaatatcagc gttatcaaac gatcagcgaa gaaccatggg aaatggattg gatccatttg
    14461 aatggtcaga acgtccaatc attttatcag gagttcacaa aagacggcca taacaccttc
    14521 cattcaacag gagatccatc agaaaacaag atccaccatt tgatcacaga attgatccac
    14581 atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct ctgtagctat ccacgagttg
    14641 ctgaatgaac tgattttgca aaagtatcag ctagatttcg aagaagagga tattcccgcc
    14701 tacgtttaca aactgaagca atacttagac gaacatttca aagaaacgat tacacttgat
    14761 caattagaac aacaattctt attgaataaa tatcagctga gcaaagattt ttccaaattt
    14821 atcggttctc caccaatcga ttatttgatc agcaaaaaac tttcttacgc taaagacctt
    14881 cttcggtata cagaatttac ggttcgaatg atttccgaag aaatagggat tgagaatttt
    14941 gcttacttta gccgcctgtt caaagcaaaa acaggactat cgccgagaga ctttcgtaaa
    15001 ttcagctgat tcatataata tgtacgtcat tttttcaaaa tattttgaaa tatatagata
    15061 aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac agcagctact gcggttattt
    15121 tttagtgatc attctcattg ttcatcttca aaatgttcat aatgaaccaa ctgtttctgt
    15181 gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact ctctcttgaa tgctttcaga
    15241 aaatgcggat aagaagaaaa accactttta ctatatacag cactagagct gcggtattct
    15301 cgcaacaagt gcttggcata gatcagcttt ttcttcatta catactgatg aaacgtaaat
    15361 ccagtgtact ttttaaattc tcgtgtgata tggtatttgc tgacaaaaaa ctgcttttcc
    15421 atttcctcta aagaaagcgg atgagataaa ttttttgaga tatattgtaa catttgttcc
    15481 acacgctcat taggaactac agcattctgt tcttgcgcac tctcttcttt ttgtacaact
    15541 tgattgagaa aaatcaagta ttctaccaga gcttgttcat attttatgtc ttcaccgtaa
    15601 atttttttat caggaggatg gtcgataaat gataattttt gcttcaatac ttcctgttct
    15661 atttgcaata cacgactcct cttctctccc attggttgaa aacaagccga tagattagac
    15721 catggcgtgg aacgactttc tagaaaagaa ggggtgacaa agatatagac acgctcgaat
    15781 acatctgaag attggcggac gatacgatgc aaatcattgg aatggatgag cagcacactt
    15841 cctgctttga tcgtaaattg tcgtccgttc aaataaaaag tcgcttctcc ctttaatgta
    15901 gcatggatct catagaaatc atgatgatga tagatcgttt tattttggct gacgacatct
    15961 ttgacatgat agatttcata ctgatcagaa accatcgtat tgacttgttc accgctgatt
    16021 ttcttttgga gcatgtgccg cacctccttt tgttcactta tgcacaattt tattatacca
    16081 cgttttttgt aatcaaaaag agctgctttt atatactctt ataaaaaaga agagacgtgg
    16141 catcttctag ccccgccccc tccattctaa atattagtta accaacgtct tctttacatt
    16201 attcaagaag acttcttcca taacgaatcc catcatcttt tgtttcttca agaaccacat
    16261 acaggtacgg acgattcttc atgatgattt ctttgatttt tgtgtactcg atcacaccgt
    16321 ctcccaaatt taccggcacg attttttcct gttcgacacg aaaatctttc aaatgaaccg
    16381 cacatatctt ttcaccaaaa cgttcaaaag cttcttctac caattggact tgttcttgat
    16441 aatcactact tgtgatcaaa ttggtcggat ctaaaatgat tcctagatag tccgaatcta
    16501 ctgcttggat cagctgctct acccttgcta aagaatacac cggatgattc aaacctggtt
    16561 ctatccccac catcatttgg tatttttctc cagcttttac caagcgacgg ataacggaca
    16621 cagcttcagc aaatgcttca tctgtgaaat tttcttccgt atattggatt tccggcaaaa
    16681 cacatccagt ttccgaagca accatcgacg caccaaagga agaagcatag cgaagatact
    16741 gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc tggatgaatc atattgatat
    16801 agcaacttaa aattcctacg gtcacttctt gcttttctaa aactcgtttg acataatttc
    16861 ccatcccaga attgatctca tttgcgccag aaggtatttc aggaaacgaa agacctaagg
    16921 cgagctgcag tgtatgaatc ccttgttctt ttgttttttc tgcaagatct tctatcgttt
    16981 ggacttcgga aagatcatgt cctctaattg taatgttcaa cataactatt ctcctaactc
    17041 agcatattgg gtagccacat gacgatttgc gggaagtaag taatcaaaaa caagacgact
    17101 aagataaccc cataaaaagg aagaagcggt ttcagcattg cctctgcttt tacgcctcct
    17161 acacttgcac cgacatatag tcctgtacct actggcggcg tgatggagcc tacacacaga
    17221 ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc ctacacttgt gacgattggt
    17281 aaaaatattg gtgtaaaaat caagatagca ggcgccacat ccataaacat acccaccagt
    17341 aataacacaa gattgactaa taacagaata ataaatttat tatccgttac gcccataatc
    17401 aacgaactaa ttgcctgagg aataccggta aacgccatgg cgaaagacat catggaagaa
    17461 gtagccaata agaacatgat cgtccctgac attgctactg attgaaacaa catattaggt
    17521 atatctttta gttttaccgt tttatagtaa aacatagcaa tcaacaggga gtaaatgaca
    17581 gctatcgctg aagcttctac tgctgtaaat atccccgtca agataccacc gataatgatg
    17641 acaatcaata gaaggctagg aatcgcctct tttattattt ttccagcatg agccatctgt
    17701 cctttttcga caacagggta tttattcttt ttagcaacga tgaatgctac tagcatgatt
    17761 cctagcgccc acaaagcgcc tacgagataa cctcccataa acaaagcaga gatcgaagca
    17821 ccaccagcaa caagtgaata catgataaaa gcagtacttg gaggaatgac catacctgtc
    17881 ggagctgacg cgatattgac cgctgtcgca aacttccggt catatccttc ttccacttgc
    17941 tgagggatca acactccgcc aatcgcggta gaagcggcaa tggccgagct agagatggaa
    18001 ccaaataaag cattccctaa cacattcgta tgagccaaag cccccggcac gcgcccgacg
    18061 aacaacatcg caaaatccac tagtttctta gctatccctc cattattcat gataatgccc
    18121 gagagaacga aaaacggaat agcgatcagc gaaaaactat tgatacttcc caccattttt
    18181 tgagctgaac taaaagcaga aacatcaaac ggtataacta acaataaagt catcatcgat
    18241 gagaatgcaa tacttatagc aatcggcatc cctaatacaa gcaacacaac aaatacaaaa
    18301 aataacacta atccggcttc tattgccatt ttattcctcc atttttatat tttttcttga
    18361 attgctggct tgttgcttaa tgtatcgatt agattgatca ggctatagat aatgatgagt
    18421 acccccgata caggtactga cagataaaca tatcccatag gaatacttaa agaaggcgag
    18481 atctgcccca ttgtcaatgt gacagcattc catccgccat acaccatgat catcgcagca
    18541 aataccagaa ataaactttg aacaacaact tctaaaattt tacttttttt gccagttaat
    18601 ttttcactta aaaccgttac ggctaaatgt gctttttgtc ccacaacata cgcggaagac
    18661 aacatggcaa accagacaag accgaatcga acaagctctt ccgtgaccgt atttggattt
    18721 cctaaaaccg ttctggaaaa tacttgccat aaaacaacag cgaccatccc aacaagcaaa
    18781 acagctccca ggatctctaa taccttgttt aaaatcattt tcatcacagc tacgctcctt
    18841 gctcttggat cttttgataa aggtctttgt aggtaggatc gtttttgaat atatcatgaa
    18901 gtggggcaac tgcctctcga aacggtgtat tatctacatc attgaattgg actccatgtt
    18961 cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc aaaagcttca atttcaaaaa
    19021 ccgttgattc tttcgctgct tcttcaatgg ctttttcctg ctcatcagtt agctgttctt
    19081 tgatggcatc attcatgatg atgatatctg gcacacgtgt atgctcatca taggaatagt
    19141 atttagccac accaccatgt ccagctgtat acaagacaaa ttcattgttt tctgatccgt
    19201 tgatcaaatt ggattgaaga gacgtgtaca cttcatcact tcccataggt acgggcgaac
    19261 cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg catcacacgg atcttctttc
    19321 ccttcaaatc ttccggcgta tggatcggac cgtctaccat ataaaagctg cgctgaccag
    19381 aatcataata ggtcaatccg gtaaatccta aatttttggt cgattggtag attggctcca
    19441 ttatttcttc attatccatc accttaaaga aatgttcttc atcattgaat agataaggaa
    19501 ctgaaaaaat cgaataatct ttagagaaac tttccaacgc tgaggcactt acttttgtga
    19561 aatctactgc tcctgactgt gtcaattcga tcagttcacg ttctccaccc agttgtccat
    19621 ctgggaaata ctggatctgt acggaaccat ctgttttttc ttttactaaa cgcccaaatt
    19681 cattcattgc atcaattact ggctggctgt tgcttgcata agcaaagcgc aaggtaacta
    19741 tctcatttac atctgcatct gcgacagttg ccacactaga attatttccg cagccgctta
    19801 gacctagcag acctaggcta agcgaacttg ctgcaaacag cagtgatgtt tttttcatat
    19861 gggtttcctt tcttttaaca ataagagacg agcaattctg tcaaacaaag caccgttaac
    19921 gactgtccat aaggcattgc tgtgattccg atattccgat aaaaatctaa actgtccccc
    19981 attcctgtcc cgattgaaac gttttgaact tctccttttt catcgatttg ttcaagcaat
    20041 ccttggatag cacgataagc gacttcttta tattcttgtg gtaaataacg tttatggacc
    20101 gctttcaaaa taccgtaagc aaatcctgca gttgcagaag attctacgta agaagaagga
    20161 tcatccaaca acgtatgcca taaaccgctt tcagactgat aagtttttaa tgcacgcact
    20221 tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat cttctttcga taattctaag
    20281 atttcaatga tctctggaat agcaatcgtc aaccagcagt ttccacgagc ccaaaatgct
    20341 tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat accacaatcc tgttcgttta
    20401 tccatcaagt atttgatatg gatcataaac tggtaacgag cttcttctaa atattctggg
    20461 cgattcaaca gtttcccgat ttttgccaaa ggaagcactg tcatcatcaa tgtatcgtcc
    20521 cacaactgat tcttattttc cggtccatat gtggcatgct gtaacccgtc ttcatttgtc
    20581 cgaggcatgt cattcatgac ccattctgcc cattgttcta aataaggcaa atagcgtgaa
    20641 tctttcgtgt cttcatacaa gtaagccata gtcaatagcg gtgccattgt attgacgttt
    20701 ttgggcggag cgccttcttc catacgatcc tcaaaccatt cattgaccac ttgataagcg
    20761 cgaggatttt ttgtcatctg gtaattttta taaataccat atagaccgac accttgtggc
    20821 caattccaca ctgtccagct tttgtcatct actttcagtc catcaaagtc caacaaaaat
    20881 tcaccattat cgtctttgat cgttgtcagg ttttcaacga gcaggtcaat tttttctttc
    20941 actaattctt taggtacaaa aatctcttct ttaataatca tctttacgcc acctttttaa
    21001 acgctttcat tttatatcct taatatagca gtaataccgt ccactataaa tatcgctttt
    21061 tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa caaaagtaac tttcagttac
    21121 ttggaaatta aaaaaagaac ttggagaaac aacgttttcc aagttctttt tcatctattt
    21181 tcttatcttt tttctacgct ttcttttgct tatccgttgt aagcatcaat gatgatctgt
    21241 ttcaattcag agatcaatgg ttctttcgga ttagctgtcg tacattggtc ttcgtaagct
    21301 aattcagcca tacggtctac ggttgtatct agtgtttctt gtgtgactcc ttgagctttt
    21361 agactcatgt tgatgccgac agattttcct aattcataaa cagccgttgc taaagcttcg
    21421 actaattcct ctgttgtttc gcctttcaga cctaagaatt ttgcgatatc tgcgtaatct
    21481 gtatctgcac ggaaatagtc atatttaggg aacatcgcat gtttttgtgg atcttttgca
    21541 ttatagcgga taatatgcgg taaaagaatc gcatttgtcc gtccatgagg gatcccgtat
    21601 tccccgccga ttttatgtgc gactgagtga caaataccta ggaatgcgtt agcaaacgcc
    21661 ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg attccatatc aggtgttttt
    21721 actgattttt ctaaatagtc aaagactaac ttgattgctt gtaggctcaa tccacgtgta
    21781 taatctgaag ccatgactga aacataggat tcgattgcat gtgttaacac atccatcccg
    21841 gtatctgctg tgacagatgc tggaactgac atgacgaatt gaggatcgac gattgcgaca
    21901 tctggcgtca atgcataatc tgctaacgga tatttcacat gtgtttcact gtcagtgatg
    21961 accgcaaatg gcgttacttc agaaccagtc cctgatgttg tcgggataca tacgaattgt
    22021 gttttttctg gtttgtcgat cttatatgtc cgtttacgga tatccaagaa tttttgtttt
    22081 gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc acattccctt agctgcgtcc
    22141 attgcagaac cgcctccaag agcgattact gtatctggct tgaagtctgc catcagtttc
    22201 gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa catctgagaa gacttcgatc
    22261 ttcacatcat ttttacgtcg tgccaatact tcacgaactg tatcgcagta accgaattgg
    22321 accataccag ggtcacaaac gatcatgaca cgttcaacgt tttccatttt ctctaagtac
    22381 aatagagagt tcttttcaaa gaagattttt ggcggtaatt tgaaccattg catgttattt
    22441 ctccgtttcg ctacagtttt gacattgatt aagtttacag cagagacgtt acgagaaaca
    22501 gagttcttac cataagaacc gcatcccaaa gtcaatgacg gaatcatttc gttatagata
    22561 tcaccgatgc cgccttctgc agatggtgta ttcactaaaa tacgacaagc tttcattcga
    22621 agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga taactgctgt atgtcctaat
    22681 cctccaagat tcaacatacc ttcacacaaa tcaaacgcat ggtccgtgtt gttagctttg
    22741 atcatggcaa gaactggaga aagtttttct cttgatagcg gataatctgg gcctacgcct
    22801 tccaattcag caatcagcat tttcgttcct tttggtacac tgattcctgc caattttgcg
    22861 atttccatcg cagaatgtcc aacgattgat ggattgaccg catattttcc ttcgttcatc
    22921 actgcatctt ctaatttttg caattcatct ggttttacga tataaacttg gtgtgcttgg
    22981 aattctgctt ttactgctgc atagatttct ttatccacga tcacagcttg ttcagaagca
    23041 caaatcattc cattatcaaa tgttttcgaa acgatcaaat cgttcactgc acgtttaatt
    23101 tttgctgttt tttcaatata agctggtacg ttacctggac ctacacctaa agctggtttt
    23161 cctgtagagt aagcagactt gaccattcca gctccacctg tcgctaagac gatcgcgata
    23221 cctggatggt tcattagcat agaagtcgct tcgatcgaag gatgttcgat ccattggata
    23281 caattttcag gagctccggc agcgatagca gcatcacgta ccactcgtgc tgcttctgca
    23341 gaagattttt gggcacttgg atggaaagca aaaacgattg gatttcttgt tttcaacgcg
    23401 atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg ttggagttac tccgcaaacc
    23461 acaccgactg gttcagcaat ttcaatcaac cctttttgga catcttcatt gatcacacca
    23521 actgttttat catgtttgat attatgccaa atatattcgg acgcatacat atttttgatt
    23581 gctttatctt cgtaaacacc gcggcctgtt tcctctacag ccatcttcgc taatggcata
    23641 tgttgatcca aagctgccat tgccatttca tgcacgatat ggtctacttt ttcttgatca
    23701 aagctttcca tttctttcaa tgctgcattt gctttttctg ccaaatcatc aatcattgct
    23761 tgtacgtcaa ttgtcttcgt tttttctttt tctattactt tagccatcat tatcctccta
    23821 atgggtcatg gttatttgtt aaataattca catgtatatc atacaatatt tatttttcta
    23881 tgtaaacact tttttgtgat ttttttcaca gttattttta aatataaaat tgctttattt
    23941 acaggtgttt gtatagaaaa atatataaag ttatttatta ttatttatat gacacacccg
    24001 attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt gaataaaaaa
    24061 tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata cttatttttc
    24121 ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt caactgtctc
    24181 agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga cagtccctgg
    24241 tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc agtctaaaac
    24301 aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc cgatcgttac
    24361 gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct gttgtttctt
    24421 ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca caagcattgg
    24481 taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac tttagcagaa
    24541 aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt agaagctttt
    24601 tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt caagcaaatt
    24661 atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt ataaattgtg
    24721 gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc ggatataggc
    24781 acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac ggaaatcatg
    24841 ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg tgccatttcg
    24901 agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa caccatcgat
    24961 caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg
    25021 tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag ataacccgcc
    25081 gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac gacgaagatc
    25141 ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat tagcatgagc
    25201 tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca cgtagtcgta
    25261 actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc ccaatttatt
    25321 ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca agtgattttt
    25381 gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa cttgaaagcc
    25441 gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc cgccagcttc
    25501 ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta aaataacgcc
    25561 agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta atgttcctac
    25621 tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc
    25681 gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg tcattcattt
    25741 ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt tcaagtgctt
    25801 tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt atcctttttt
    25861 tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata catggtatat
    25921 tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat taaaacgcca
    25981 agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc taatccctac
    26041 attgattggt attgctacta ttcttcttat tatgattcct attatcttgt ttgccaccac
    26101 catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg gtagttcagg
    26161 aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg gtatttcttg
    26221 gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca aagatgcatt
    26281 tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat
    26341 ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa gctattctta
    26401 ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa cacctaaagt
    26461 actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac aacttttctg
    26521 gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg gtattggtta
    26581 tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac aattgccaaa
    26641 agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac accgtttatc
    26701 gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac aacattcatg
    26761 tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa cagttaatca
    26821 ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca ccgcgattgg
    26881 tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa
    26941 tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg ccagtaacgc
    27001 catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt ggctgctgcg
    27061 tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa cagtttgacc
    27121 gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac ctaaataata
    27181 accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt gcaataaacc
    27241 tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc cgtgttccgc
    27301 tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga caaacagtag
    27361 ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa caataaagat
    27421 ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct gataaaccga
    27481 acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc
    27541 tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa cgttacttgc
    27601 tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg cacccgtacg
    27661 ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat agacagagag
    27721 agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg atcctgaaag
    27781 tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg cagtcaccac
    27841 atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct tgattgctcc
    27901 tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt
    27961 ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat aaccaacagc
    28021 aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt gttctacatc
    28081 gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg agatgtattt
    28141 ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt
    28201 tactcgtgga tatttttctt ctaattctgc ctttgtatga taattctctt caacagccct
    28261 tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt cttgctcgac
    28321 cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt tcgtcattga
    28381 atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc gcatttcatt
    28441 ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta gcgtttcaac
    28501 tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag
    28561 tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca aggaaatcga
    28621 agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa atactaaagc
    28681 cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc ggacctgtaa
    28741 aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca tttctgtagc
    28801 catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac atcctttaaa
    28861 taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg ctgcatcttt
    28921 ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt gtgatgcttc
    28981 gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa tatcacccac
    29041 taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag ttgccgtttg
    29101 tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt ggacaatcgc
    29161 caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata aatccccatg
    29221 ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga tgatcgttgt
    29281 aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg cctcatcgaa
    29341 tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca acccagtatc
    29401 tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt cttccatcct
    29461 attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa catccttttt
    29521 ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt
    29581 gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga aggaggatac
    29641 catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag
    29701 tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga atgcgtatgg
    29761 atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca attgaataac
    29821 gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag atgtaacgaa
    29881 aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa
    29941 gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg agcttatcga
    30001 gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag
    30061 cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg
    30121 ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac
    30181 taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt
    30241 gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc
    30301 attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat
    30361 tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta
    30421 gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc
    30481 taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag
    30541 taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc
    30601 atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg
    30661 catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat
    30721 ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga
    30781 gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc
    30841 ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata
    30901 taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc
    30961 ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag
    31021 taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc
    31081 aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca
    31141 ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt
    31201 ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat
    31261 agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg
    31321 tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag
    31381 ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg
    31441 atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa
    31501 gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg
    31561 atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt
    31621 caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag
    31681 atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt
    31741 cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat
    31801 ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa
    31861 acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc
    31921 atcatgagtg aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct
    31981 gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat
    32041 gcgacaagag tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca
    32101 ttacaagaag caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat
    32161 aattcgaatg tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa
    32221 gtatccagtg tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat
    32281 cttcgtgata ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat
    32341 gaacagaaca aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat
    32401 gtgatttggg gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa
    32461 tctaaggagg tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca
    32521 aaaggttttg aacatcttta tatttcagga cttgctttga tcattggtat catcattgct
    32581 gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta
    32641 accagtgcct tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt
    32701 ggcgtaggta aattacctgc gattatcgca ctctttattt attcattatt gccgatttta
    32761 cgtaatacgt acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc
    32821 atggggatga ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg
    32881 atcatggctg gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct
    32941 tatatcggcg ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca
    33001 gatctcattt tggcaggtac gatccctgta acaattttag cactattggc tgattggctt
    33061 ctaggtctct tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga
    33121 aacgtatact aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct
    33181 tccctggtct tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt
    33241 cagaagcaca gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa
    33301 ataccgcaat catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg
    33361 gagatgcttc aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc
    33421 ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa
    33481 gatacggtca aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc
    33541 gaaaagacac cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg
    33601 atgaattagt agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg
    33661 gcttccaaga aacttatggc ttctccttca attcgatatt acctatgcag attggacttg
    33721 tttatgacgc agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac
    33781 ggatcgccag ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg
    33841 atgctgctcc agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct
    33901 taaataagtt aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag
    33961 ataataatct agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt
    34021 ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt
    34081 ccaggaaaat ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta
    34141 tggtgtcctc tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg
    34201 taaattagca aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc
    34261 catgatctcc attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt
    34321 atttttgtat tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga
    34381 caaaaacatc cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat
    34441 ggtagagtta ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt
    34501 tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat
    34561 tatccgagga accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac
    34621 tgcattaatg gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc
    34681 agccagccgt acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt
    34741 tggtcgcaaa ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc
    34801 cctccttatg ggcaaagagt tgcttttctt agctatttat ccattccgta tactccaact
    34861 tcatactgcc aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa
    34921 ggcattttgc caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta
    34981 aatactcgtc tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt
    35041 tcatttaagc cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa
    35101 ctggtgactg ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc
    35161 tcatccttgg ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt
    35221 tcctaaaacc ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac
    35281 gatgatcctt caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag
    35341 aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat
    35401 tcatggccga aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca
    35461 atgtcgcttt ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg
    35521 tatcttttcc aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt
    35581 gattgtccat tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa
    35641 ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg
    35701 atgctccaat tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc
    35761 gtcaacattc cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa
    35821 attaaagaaa tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc
    35881 tttttgggga gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata
    35941 atgtgtccag gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac
    36001 tcgatgctgt ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc
    36061 aaaagactgc gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg
    36121 tttctttaaa ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc
    36181 gactgtatag ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta
    36241 ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg
    36301 gtttcttgaa tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga
    36361 tcactaataa tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa
    36421 tttttacgag cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct
    36481 gatataatta tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt
    36541 cagcaaactt ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg
    36601 tccacgctca cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa
    36661 caacgtatca tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca
    36721 atacacgaag gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata
    36781 cgcacgaaag atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc
    36841 ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt
    36901 gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa
    36961 aaatggtcac ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc
    37021 gaggtaaagg aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga
    37081 aattatgatc aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca
    37141 tttcatacgc tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga
    37201 caaaacagac cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt
    37261 ggtttatttc ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa
    37321 tcgcaatctt aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg
    37381 tacaaattga ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac
    37441 gatatctaca taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga
    37501 aaaacggaca tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac
    37561 atcttcgtct agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt
    37621 caacgcaccg atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc
    37681 ataatcatgt agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg
    37741 atcatggatc ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat
    37801 gtcccctaac cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt
    37861 aatattggca atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat
    37921 ccctgtttga gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg
    37981 ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat
    38041 cccattggaa tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc
    38101 ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc
    38161 taaaatcagc gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc
    38221 tttaccaatt tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt
    38281 gataaatgtg atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc
    38341 agcggcattt tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac
    38401 cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc
    38461 cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt
    38521 atataaacca acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa
    38581 taggaacgat gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat
    38641 tgtcacagaa atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc
    38701 agcacctgct gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc
    38761 ggaaatcaag ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc
    38821 atgaggataa gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat
    38881 aacaaaagcg gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac
    38941 gacaacgatt tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc
    39001 caaagcagca aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc
    39061 tactaacaac ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca
    39121 agttttatag tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa
    39181 agtcaataaa ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca
    39241 aacgctttaa tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata
    39301 ataaaaggat agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt
    39361 tggaagagag atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca
    39421 gttgtcacaa cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa
    39481 ccaaattaaa aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc
    39541 agctttttta caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca
    39601 aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa
    39661 acactgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt
    39721 gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac
    39781 aacaaacaaa ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc
    39841 tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg
    39901 gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac
    39961 taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac
    40021 gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg
    40081 gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc
    40141 cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg
    40201 gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac
    40261 ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata
    40321 aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc
    40381 tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc
    40441 gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc
    40501 agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc
    40561 ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa
    40621 tttttgctga atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt
    40681 gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat
    40741 tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt
    40801 cgttgagctg agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc
    40861 agtcccaatc gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc
    40921 tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag
    40981 caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg
    41041 gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa
    41101 tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc
    41161 attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt
    41221 ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc
    41281 tgtcgttgtt gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct
    41341 tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc
    41401 ctgattttct aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa
    41461 gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc
    41521 gtcgatctct gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc
    41581 aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt
    41641 ttcacccatc tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt
    41701 ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat
    41761 cgtgtccaaa ttttcatctg ttgataaaat catatcttta actttattga tacttactag
    41821 gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt
    41881 tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt
    41941 cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc
    42001 accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta
    42061 atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc
    42121 taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc
    42181 ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc
    42241 atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat
    42301 cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata
    42361 taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga
    42421 attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt
    42481 ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg
    42541 attacagcct ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt
    42601 atctattata cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat
    42661 gtttcagctt ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt
    42721 ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa
    42781 gcagacagat tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg
    42841 ccagcaaagg cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct
    42901 tccttttttt gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct
    42961 ggtttcatga tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca
    43021 aggtctgcct gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct
    43081 gaagctccta atttttttac cttttcctca tttttactgg atgcactggt taagattcgg
    43141 attccctttt catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt
    43201 atcattacag tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta
    43261 attcctggcg tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct
    43321 acttttgctg atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct
    43381 actcgatcac ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg
    43441 ccatcattac ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa
    43501 cgcaaggcaa catcataagg attcacactg aatgctttta tttctacacg aacatgtcca
    43561 gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa
    43621 ccgttaattg caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat
    43681 gcctttattt tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag
    43741 ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta
    43801 cgctaataaa aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag
    43861 caaaaaaaca gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc
    43921 aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa
    43981 atcgtgattt aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta
    44041 taaaattccg tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt
    44101 aatccaagtc tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca
    44161 aaaaagatac gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt
    44221 tttacgcaca gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta
    44281 tttagtaaca ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc
    44341 tgccataatg ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt
    44401 tctgatctgc tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat
    44461 cccaatgtga gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat
    44521 cgtcgggcta aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc
    44581 ttgctccgat cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat
    44641 aggatcctcc ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt
    44701 catagtggat cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat
    44761 ttttgccgat aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca
    44821 gttgatccat acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa
    44881 aaccctgcat ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact
    44941 ctgcttcagg agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt
    45001 tgacttgaat caattcagaa accagctcaa gtgctggctg caatggataa acttccggta
    45061 gtgcgtgccc agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga
    45121 ttgtttcatc atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt
    45181 cgggaagttt tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat
    45241 ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt
    45301 ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca
    45361 tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa
    45421 cagcagctgc tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa
    45481 cagctacagt cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc
    45541 cagcttctct caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac
    45601 ctgcaactgc tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta
    45661 ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta
    45721 tagctttttt atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc
    45781 cttctttcta gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac
    45841 tgacccatac attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt
    45901 ttaaaatctc gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga
    45961 aagcctcttt gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg
    46021 actggaatag tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt
    46081 cgatcagccg agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat
    46141 gcttttctct tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc
    46201 cacaaataag tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat
    46261 tttatgttta atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt
    46321 taccgccatc tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac
    46381 gataattacc gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat
    46441 tgtatccttt tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat
    46501 cagctggatc ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt
    46561 cttctaataa tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg
    46621 tttcgatttg ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg
    46681 gacgtaaagt cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt
    46741 catttggtgt aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga
    46801 tacggtgaac gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac
    46861 ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc
    46921 tcttaaatga acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt
    46981 ctgcacggta accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac
    47041 gtccgaagac gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga
    47101 atttttcgta ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt
    47161 tcgctttgat cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag
    47221 ctggcggcat cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga
    47281 ttttttcgat atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta
    47341 atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg
    47401 gtgtacccac aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg
    47461 ctccgccgta aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt
    47521 cttgtgtttg gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac
    47581 gatctggatc gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg
    47641 tctgtgcttg tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga
    47701 taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt
    47761 ctagttcttt aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt
    47821 tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg
    47881 cacgttttta tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta
    47941 ggcattagaa aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc
    48001 aacaagttgt ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta
    48061 ttctaacata gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt
    48121 gttctaccac ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg
    48181 cttcaagatt ttgtttgata gcatcaatca atttattctt ttctttttca aaatagaaca
    48241 caggtagttg ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat
    48301 aaaggaaaat catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat
    48361 ttaaagactc tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg
    48421 ttggcagatt tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga
    48481 catcgctcaa aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga
    48541 tagaaacatg ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga
    48601 tcgtatacgc aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg
    48661 tttctccttc tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa
    48721 catcgccatt tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg
    48781 tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga
    48841 tttccatttt ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac
    48901 cgatcattgt gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat
    48961 tggcgtgatc catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa
    49021 tcgtgtaagg aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt
    49081 tttcagacaa gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa
    49141 ttgcaacgac ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt
    49201 tcaaaggatc tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc
    49261 tccaaaatga agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc
    49321 gcgtaccagc aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga
    49381 cattggtaat ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta
    49441 ttgttagatt cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac
    49501 ggcgggttcg catgactctc acttccaagt aaatttctct attcgtattt agaagctaga
    49561 atgaacgcgg ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc
    49621 aaaaaaagat tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa
    49681 atcaccgtat ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa
    49741 tcctggcata gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc
    49801 gcaccggctc aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc
    49861 tttttgataa acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat
    49921 atattttgct tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt
    49981 ccgaacgtct tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc
    50041 aacgattgct cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg
    50101 gttttctgct tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg
    50161 ataaagcaaa gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt
    50221 cttaagtact ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt
    50281 catgatttta tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt
    50341 caatgtctct aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg
    50401 aaaaacgatg gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc
    50461 ttcctctgtc tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt
    50521 ttctgttagt aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac
    50581 ttggtaataa ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc
    50641 atgctctgcg ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat
    50701 tctaaatcgt tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac
    50761 ttatttataa aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc
    50821 tagattattt gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg
    50881 gcgcaaacaa atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat
    50941 gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa
    51001 ctaggcccca ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct
    51061 tctgccttca ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca
    51121 acggtcaaaa agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat
    51181 ttttcaaaat cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag
    51241 aacggtgacg cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt
    51301 gatcttcaac tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta
    51361 gttgatctaa aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc
    51421 ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc
    51481 cgcagcccca agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg
    51541 tctggtaaaa acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact
    51601 ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg
    51661 aaacaggtgt ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc
    51721 catgtactaa caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa
    51781 aaagagttcg gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca
    51841 tataccatca actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa
    51901 aaactcacac aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg
    51961 gtccttcgac tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg
    52021 acaagttttc ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta
    52081 ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg
    52141 cttttgctga aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt
    52201 tccctcatgg aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta
    52261 gaagatacat tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag
    52321 atccctcaac agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat
    52381 ttggaattga aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt
    52441 gttttcggta tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca
    52501 cctgtagaaa acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca
    52561 ttcgtcatta tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag
    52621 tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa
    52681 ttgtttctca aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca
    52741 ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg
    52801 gaacatggaa cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat
    52861 aaaatacctt cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt
    52921 taacgcttga cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct
    52981 tatgccagct tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt
    53041 cgcttcgtca aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag
    53101 tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat
    53161 tttataatcc gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta
    53221 ttctttggtc gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc
    53281 tcctaatttt atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat
    53341 tctttttcgc cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa
    53401 cattgatcca ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt
    53461 tcagcgtgat caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa
    53521 gcgtcatcaa taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa
    53581 cgatccggcg atccattgga atagctgtat cagtttgata atattcctta tttagccagc
    53641 tgcctgttac tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa
    53701 tcaagaggac caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg
    53761 ttaaacgttt caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag
    53821 gcaaatacat aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc
    53881 taaataaaaa aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc
    53941 ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca
    54001 gcaagaaacc ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc
    54061 tcaaaagaaa caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca
    54121 gatttctttt gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt
    54181 aggccatgac gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag
    54241 gcaactcaat cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc
    54301 catccatatt gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta
    54361 caagccatgc tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa
    54421 atacgcctaa tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca
    54481 acgtgtaact atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa
    54541 aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca
    54601 aaaataaaag ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg
    54661 taaaattacg cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct
    54721 tataactaat ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat
    54781 atattcttgt aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta
    54841 cttgattact caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc
    54901 cggcaggtga taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct
    54961 gtaaaccaga gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt
    55021 atgcgagagc ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa
    55081 taagttcttc tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc
    55141 cctgattcac tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc
    55201 agcgaaaaac tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac
    55261 ttaaataaat ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc
    55321 tttttaataa aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact
    55381 gataagtttt cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaaacttccg
    55441 gccatacttc ttgtatgact gccatcagct catccagttc attttctgtt acttttattt
    55501 tttccataca cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa
    55561 agagcatgat catgctatac acacccaccg accacggttt caagaaaatc acaatctgca
    55621 taaattccct aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg
    55681 caaagggaag cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca
    55741 gcaattcatc aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc
    55801 catagtaacg gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat
    55861 ataaaagccc aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg
    55921 gcaaaatagg aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact
    55981 tgccaaattg ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat
    56041 aagcataaag aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac
    56101 atttttgtaa aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat
    56161 taaaactgcc tcct
//
LOCUS       DAYYCM010000011        55830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000011 DAYYCM010000000
VERSION     DAYYCM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.011"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            443..763
                     /locus_tag="ACHAY1_000757"
     CDS             443..763
                     /locus_tag="ACHAY1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8592776.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            917..1822
                     /locus_tag="ACHAY1_000758"
     CDS             917..1822
                     /locus_tag="ACHAY1_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592777.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1819..2592
                     /locus_tag="ACHAY1_000759"
     CDS             1819..2592
                     /locus_tag="ACHAY1_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8592778.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2773..4197)
                     /locus_tag="ACHAY1_000760"
     CDS             complement(2773..4197)
                     /locus_tag="ACHAY1_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8592779.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4408..4854
                     /gene="nrdI"
                     /locus_tag="ACHAY1_000761"
     CDS             4408..4854
                     /gene="nrdI"
                     /locus_tag="ACHAY1_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8592780.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4919..6901)
                     /locus_tag="ACHAY1_000762"
     CDS             complement(4919..6901)
                     /locus_tag="ACHAY1_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8592781.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6915..7811)
                     /gene="murQ"
                     /locus_tag="ACHAY1_000763"
     CDS             complement(6915..7811)
                     /gene="murQ"
                     /locus_tag="ACHAY1_000763"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8592782.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7814..8875)
                     /locus_tag="ACHAY1_000764"
     CDS             complement(7814..8875)
                     /locus_tag="ACHAY1_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8592783.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9086..9613)
                     /locus_tag="ACHAY1_000765"
     CDS             complement(9086..9613)
                     /locus_tag="ACHAY1_000765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8592784.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9680..11182)
                     /gene="eat(A)"
                     /locus_tag="ACHAY1_000766"
     CDS             complement(9680..11182)
                     /gene="eat(A)"
                     /locus_tag="ACHAY1_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8592785.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11440..12561)
                     /locus_tag="ACHAY1_000767"
     CDS             complement(11440..12561)
                     /locus_tag="ACHAY1_000767"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8592786.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12687..13628
                     /locus_tag="ACHAY1_000768"
     CDS             12687..13628
                     /locus_tag="ACHAY1_000768"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8592787.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13778..14674)
                     /locus_tag="ACHAY1_000769"
     CDS             complement(13778..14674)
                     /locus_tag="ACHAY1_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8592788.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14943..16283
                     /locus_tag="ACHAY1_000770"
     CDS             14943..16283
                     /locus_tag="ACHAY1_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8592789.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16501..16944
                     /locus_tag="ACHAY1_000771"
     CDS             16501..16944
                     /locus_tag="ACHAY1_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8592790.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17448..18866)
                     /gene="nhaC"
                     /locus_tag="ACHAY1_000772"
     CDS             complement(17448..18866)
                     /gene="nhaC"
                     /locus_tag="ACHAY1_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8592791.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19269..20492)
                     /locus_tag="ACHAY1_000773"
     CDS             complement(19269..20492)
                     /locus_tag="ACHAY1_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8592792.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20523..21362)
                     /locus_tag="ACHAY1_000774"
     CDS             complement(20523..21362)
                     /locus_tag="ACHAY1_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8592793.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21379..22059)
                     /locus_tag="ACHAY1_000775"
     CDS             complement(21379..22059)
                     /locus_tag="ACHAY1_000775"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8592794.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(22061..23134)
                     /locus_tag="ACHAY1_000776"
     CDS             complement(22061..23134)
                     /locus_tag="ACHAY1_000776"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8592795.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23190..23428)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23471..23704)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23781..24452)
                     /locus_tag="ACHAY1_000777"
     CDS             complement(23781..24452)
                     /locus_tag="ACHAY1_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8592796.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24539..27187)
                     /gene="mgtA"
                     /locus_tag="ACHAY1_000778"
     CDS             complement(24539..27187)
                     /gene="mgtA"
                     /locus_tag="ACHAY1_000778"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8592797.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27529..27703)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27876..28313
                     /gene="ndk"
                     /locus_tag="ACHAY1_000779"
     CDS             27876..28313
                     /gene="ndk"
                     /locus_tag="ACHAY1_000779"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8592798.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28335..29201)
                     /locus_tag="ACHAY1_000780"
     CDS             complement(28335..29201)
                     /locus_tag="ACHAY1_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8592799.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29361..31454)
                     /locus_tag="ACHAY1_000781"
     CDS             complement(29361..31454)
                     /locus_tag="ACHAY1_000781"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8592800.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32203..32826)
                     /locus_tag="ACHAY1_000782"
     CDS             complement(32203..32826)
                     /locus_tag="ACHAY1_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592801.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32985..34382)
                     /locus_tag="ACHAY1_000783"
     CDS             complement(32985..34382)
                     /locus_tag="ACHAY1_000783"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8592802.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34414..35385)
                     /locus_tag="ACHAY1_000784"
     CDS             complement(34414..35385)
                     /locus_tag="ACHAY1_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8592803.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35759..36184
                     /locus_tag="ACHAY1_000785"
     CDS             35759..36184
                     /locus_tag="ACHAY1_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592804.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36672..37373)
                     /locus_tag="ACHAY1_000786"
     CDS             complement(36672..37373)
                     /locus_tag="ACHAY1_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8592805.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37389..37829)
                     /locus_tag="ACHAY1_000787"
     CDS             complement(37389..37829)
                     /locus_tag="ACHAY1_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8592806.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37829..38404)
                     /locus_tag="ACHAY1_000788"
     CDS             complement(37829..38404)
                     /locus_tag="ACHAY1_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8592807.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            38645..38854
                     /locus_tag="ACHAY1_000789"
     CDS             38645..38854
                     /locus_tag="ACHAY1_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8592808.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(39252..39764)
                     /locus_tag="ACHAY1_000790"
                     /pseudo
     CDS             complement(39252..39764)
                     /locus_tag="ACHAY1_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39890..40591)
                     /locus_tag="ACHAY1_000791"
     CDS             complement(39890..40591)
                     /locus_tag="ACHAY1_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8592809.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40699..42142)
                     /locus_tag="ACHAY1_000792"
                     /pseudo
     CDS             complement(40699..42142)
                     /locus_tag="ACHAY1_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(42156..44021)
                     /locus_tag="ACHAY1_000793"
     CDS             complement(42156..44021)
                     /locus_tag="ACHAY1_000793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8592810.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(44142..44992)
                     /gene="licT"
                     /locus_tag="ACHAY1_000794"
                     /pseudo
     CDS             complement(44142..44992)
                     /gene="licT"
                     /locus_tag="ACHAY1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(45302..46015)
                     /locus_tag="ACHAY1_000795"
     CDS             complement(45302..46015)
                     /locus_tag="ACHAY1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8592811.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(46083..46409)
                     /locus_tag="ACHAY1_000796"
     CDS             complement(46083..46409)
                     /locus_tag="ACHAY1_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8592812.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46532..46759)
                     /locus_tag="ACHAY1_000797"
     CDS             complement(46532..46759)
                     /locus_tag="ACHAY1_000797"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8592813.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(47081..48334)
                     /locus_tag="ACHAY1_000798"
     CDS             complement(47081..48334)
                     /locus_tag="ACHAY1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8592814.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48395..49135
                     /locus_tag="ACHAY1_000799"
     CDS             48395..49135
                     /locus_tag="ACHAY1_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8592815.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49221..49979)
                     /locus_tag="ACHAY1_000800"
     CDS             complement(49221..49979)
                     /locus_tag="ACHAY1_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8592816.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49976..50440)
                     /locus_tag="ACHAY1_000801"
                     /pseudo
     CDS             complement(49976..50440)
                     /locus_tag="ACHAY1_000801"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(50459..50956)
                     /locus_tag="ACHAY1_000802"
     CDS             complement(50459..50956)
                     /locus_tag="ACHAY1_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8592817.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(51024..54191)
                     /locus_tag="ACHAY1_000803"
     CDS             complement(51024..54191)
                     /locus_tag="ACHAY1_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8592818.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54415..55299)
                     /locus_tag="ACHAY1_000804"
     CDS             complement(54415..55299)
                     /locus_tag="ACHAY1_000804"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8592819.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55394..>55830)
                     /locus_tag="ACHAY1_000805"
     CDS             complement(55394..>55830)
                     /locus_tag="ACHAY1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592820.1"
                     /translation="EWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVL
                     VLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLC
                     FYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        16056 a        11744 c         9607 g        18423 t
ORIGIN      
        1 ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat agacactatt
       61 tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg taaggtaaag
      121 ctttttttaa tttattgccc gataatatat agaaataagg tctagttact gtttttttta
      181 aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc ttcttttccg
      241 ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga ttgccgatat
      301 ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg taggataaag
      361 aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt gtattttttt
      421 atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag gaattttgga
      481 agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca attattcttt
      541 ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa tcaaaagttt
      601 gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt cggtcataat
      661 tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg tggggatgct
      721 actcatcagt atcattggca tcaagattac tagcggccac tagacacttg gaataagtac
      781 tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa aaataagaaa
      841 taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac tatctataga
      901 aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga actttggtca
      961 caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg ttggattagt
     1021 aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac tgatccatta
     1081 ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc ataaagggtt
     1141 ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa caggctatga
     1201 tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta tcgtgaatca
     1261 gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg gaatgaaaca
     1321 aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt tagatgagcc
     1381 gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta ccgacttggc
     1441 tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg aaaaaatggt
     1501 cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa tggctgaatt
     1561 gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag ctgctcgaac
     1621 gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc ttttaaaaga
     1681 aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata tccatgtgac
     1741 agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc tggattcagt
     1801 aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa attgatcaag
     1861 aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg aacagcactt
     1921 ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca gctttttagt
     1981 gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat ttcgatggaa
     2041 gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg agaaatatta
     2101 gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt agctattatt
     2161 gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga gaaggtttct
     2221 actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta tatcggttta
     2281 tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc cggcgcatcc
     2341 atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat ccttattgca
     2401 ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa tctgcaaaac
     2461 caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa tcaaatgcta
     2521 ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt atttaagcga
     2581 aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag ccttattgcc
     2641 gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa catttgtgtc
     2701 acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc tctttatact
     2761 tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg caatcactcc
     2821 gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta caggctcgtt
     2881 ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa caagaacaag
     2941 cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac ttgctaaaag
     3001 ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt ggtacaatcc
     3061 taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa agttataagc
     3121 actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg tcaatacgat
     3181 agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca aaagtccgtc
     3241 ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc aaacaccaac
     3301 taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa ccatcatttc
     3361 aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca tcgtcattgt
     3421 cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata tccctagaat
     3481 cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag aggctccttc
     3541 aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac tggaaaacgg
     3601 gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa tgaattgttg
     3661 gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta atttacagaa
     3721 tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta gccatgcata
     3781 tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac cttcaaatac
     3841 tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt tgcctaacca
     3901 gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa aaccttcttc
     3961 tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat tattgatcga
     4021 caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag ctgggccgcc
     4081 agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg ctgaaccaat
     4141 tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg tatccatgta
     4201 agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt tttattttta
     4261 cttaattaat aaaaacttta atagaaatat aattctaccg gcttttttct cattatacaa
     4321 gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa gatgctatac
     4381 taaaaggaaa gaattggagg atgatccatg aacattttat atatatcgat ttcaggaaat
     4441 acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc agaagatcct
     4501 gtcaatcctg aagttacttt aaaagaaatc catgaaaata gtgattttac gaaagaaaca
     4561 gaaccatttt ttactttcgt tcctacttat ttagacggcg gaaatggttt agataacggt
     4621 gatacagaaa tcttgaccga aacgatgcgt gaatatctgg aacatgaaga caatcataaa
     4681 ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca ataatcagta ttgtctaaca
     4741 gccaaacaat atgcacaaac atttggcttt cctttcttgg cagattatga attacgggga
     4801 acaccatctg atgtgacacg aatttatcag attttagcag aaaacaataa ataataaaaa
     4861 agaagaagct gaaaaataga tttcagtttc ttctttcgtt tctattgttt ctatcatatt
     4921 aagctttgac atccatcaca ggtgcttttg cttttgcttt acctgttttt tccaagacaa
     4981 agttttttac acgatccata ttggttacca agacgatcat ggatgtttct ttgcctgctg
     5041 ctttgatcgc ttctaagtca gcttctgcta caagcgtatc aggtgtcact tgctgccctt
     5101 ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt cccatatgaa
     5161 gcaagatttc taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt gggaagacag
     5221 agctgactac cccagtaact ggagaataga tatgtccgtt ttctggttct acaaagtagc
     5281 cttcacccat catcttttgt gcaaaaactg gatcacttgc tttatctaaa gaaacaactg
     5341 ttccgtcagc aactgatgag aggatagtca cactttcagc tgaagtttga ggtactactt
     5401 tttcaggcac ttcatttact gtggctgttt cagtcgctaa ttcttctttt tctaaacggt
     5461 ctttaggaat accgaagaag aatgtagcaa cgaagccacc agcataagct gccaataatc
     5521 caaggatgta gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg gctcctgaag
     5581 gtccaatagc gatagccccg acatttccga tcattccgat cactgccccg ccgatacctc
     5641 caccgataca agcagtaata aatgggcgtc ctagaggtaa tgttactccg taaatcaatg
     5701 gttcgccgat tcctaggatc ccgactggta aagctccttt gatcatttct gttaattctt
     5761 tatctttttt gcagcgaatc cataatgcca atgctgcacc cacttgtcct gcaccagcca
     5821 tcgcaagaat tggaagcaat agcgtacgac cagtttcatc aatcatttgc acatggattg
     5881 gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg cctagaacga
     5941 agccggcaaa tgctccacca acgccaagta cccagttgat cccaccgacc atgccatcag
     6001 aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat cagaccgatg gcaatcaatg
     6061 aaagagttgg tgtaacgatg atatctaccg catcaggtac tactttatgc agttttttct
     6121 caacgattga caatagccaa acagcgaaga tgacacctag gatcccacct tgtccagctg
     6181 caagagcttc accagtaaat aagtttttca gtggtgcttc cggattcatc cctgtcagca
     6241 tcacaacggc cccgataact ccaccaagag ttggtgttgc accaaaaact tgtgcagcat
     6301 tgatacctgt atagataact aggtagctaa acatcccgtt tttcaagatg ttcatcacat
     6361 cgatatattg ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag tttgctaaaa
     6421 ctgcggcaat ccctgccacg atcccagtac cgacaaatgc aggaatcatt ggcacaaaga
     6481 tattagaaat gtcttttaat accgatttga ttttagatgg tttttgtttc gatttttgtg
     6541 ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt tccagatgct gatgaatttg
     6601 ctgtagctgt acttacagaa gaaagttttt ctcctaattt cacgcctgcc aaatcaacca
     6661 tttgctgtgc tactttgttc actttcccag gtccgatgat gacttgaagc tgttcatctt
     6721 ctactacacc aagcactcct ggaatcgctt ttaattcaga gacatttaca agatcatcat
     6781 tgatgatctt catacgcaca cgagtcatgc agtggacaat gctttctaca ttgcccatgc
     6841 cgcctgcccc tttataaatt tgttttgcca gttcttccaa agtcagttcg ttttttgcca
     6901 tggtcttttc cccctcataa ggtccctttt acaaatccgt ttgcttcagc taattttgct
     6961 tgagcttcct ctttatcact atttgtcaaa atcatgacga tagccagctt aacattttga
     7021 tctgcttctt caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc ttgcatgatg
     7081 attcgttttg agcgttcgat tagtttttca tttgttggtt tgacatcgac catcaagtta
     7141 ttgaatactt tacctatacc aatcatactg atagtagaaa tcatattcaa gataagtttt
     7201 tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa attctggacc tgcatcgact
     7261 tcgattggga aatccgcgtg tttactgatt tcggcttgtt tattgcaaga aatcgttcct
     7321 gtgacagcac cgatcgattt tgcgtaatct aatccaccga tcacatatgg tgtacgaccg
     7381 ctagctgcga taccaataac tatatcattt tttgtcaaat tcagcttgat tagatcttct
     7441 tgccctagct cataagaatc ttcggcccct tcgacagcta cagtcattgc ttcttctcca
     7501 ccagcaatta gacctatgac catttccggt tctacaccga atgtcggtac gcattcagca
     7561 gcatctaaca cacctagtct tccacttgtt ccggctccca tataaatcag acgtccacct
     7621 tttttaaagg actcaatcgt gttagcaatc actggttcga tacttgtcag ttctttttca
     7681 accgcagcgg ccactttctg atcttctttg ttcattagtt gaacggcttg agaaatactc
     7741 atctcatcta atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt ctctaaattg
     7801 attttgctca tttttatctt ccttcttttc ttataaattg atagttcata ccagctttga
     7861 tttggttgat caatggtagc tcttctttga taactttagc tactacattc actttttcat
     7921 ccgctggcaa atcatatttg gttaattgga tctcacccat atagcgcaaa tacttttcgt
     7981 tatctaaagt aatagaccct ttcatacgcg tagctgtatt tcttgctggg ataggcggaa
     8041 tctctcgaaa acgtgcatct acgcttcgta tgacatctct tgcatcgtct tgccgattcg
     8101 tatgcttccc taatatatat tcgtaaaatt cttcatctac aggttccgta tggagcaaga
     8161 ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac gttttctgac agacctgcat
     8221 ctccgatgta aacaatatcc gtgttacttg ctaatagatc tagagcgcct gcaagaggat
     8281 ggatccctcg atgttcttcc aaagttggca atccttgata tagtgggcct ctcaactctg
     8341 tatctcctgg tacaaaacct tggacaagga gaccttgaat tttgagccat tgattttttt
     8401 tctgatacca ttctttgtcc agccctgttt cgggacgtgg ataataatta tgccaagctt
     8461 caatctgtga gaaatcagct tctgcttctt tcaattcatc gatatcttta ggcgtgatcg
     8521 tgctagcatt caaactgatt ttcaattgat gtgaccagtc ggcaatctgc tgattggaaa
     8581 tatgataatc cattcttaaa cctgttacac caattgcttt caaaggcgtt agatcttcta
     8641 atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat tctagtttat
     8701 tctttttcgc ttcgcttcct agttcgatca atcgcttttg atatgccgaa acatcatctt
     8761 ctggtatatg catggaagtg aaaataccga caaagccatt ggatgccatt tggcggatat
     8821 actgtttctt ctcttctgac aaagaatcgt tcataaatat tgaaaagcca aacatattat
     8881 ccctcttttt ctgtatccgt cttcatttct atgactagag tatatatcta aagaaacaaa
     8941 atttcaatac ttttattaaa ataattaaat ttcatttcta aaggttgagt ttatattatc
     9001 aaaagaattg tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc ttgaaatatt
     9061 ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta ttatctacca
     9121 tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc actcttatca
     9181 aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat tctttcaata
     9241 aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat cgtacagcga
     9301 acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca taatccccaa
     9361 aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga tcagcaactg
     9421 cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg attggaacag
     9481 gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt tcagtcagcg
     9541 gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct ccgtgtctcg
     9601 ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc ataccttgtt
     9661 gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta gatactttat
     9721 ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc aagatcagtt
     9781 gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc catgtatata
     9841 gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg cccatactca
     9901 tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg aggttgttca
     9961 aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt cctcgattgt
    10021 cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat ttttctccta
    10081 taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt cctgcaccgt
    10141 taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt ggctcgaata
    10201 acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta tggtgagacg
    10261 cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt ttttctttct
    10321 cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc gtccgtgcag
    10381 catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg aatccgactt
    10441 gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca gctgctgttt
    10501 tctttagcct actgacttct tttttcaatt tttcattttg agccatttca aattcatcac
    10561 gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc agttgacttt
    10621 tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca tggctgacca
    10681 cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta ccagagatat
    10741 ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca aataaaagtg
    10801 ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc cataagattt
    10861 caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca aaatccgtaa
    10921 tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc gggaaatagg
    10981 caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt agaatattca
    11041 gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa
    11101 aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg aatgtcagat
    11161 tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag tcctattttc
    11221 agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat acatcgaaaa
    11281 aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg tacagtctga
    11341 atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata
    11401 tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga ggatggtgaa
    11461 aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc tgtgttccat
    11521 tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga gaaaatcaga
    11581 aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt ttcgatattt
    11641 ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct gatcgatccg
    11701 cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc cagtgatttg
    11761 gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg ctccaccaat
    11821 accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga aatcttttat
    11881 ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat ctattcctcc
    11941 tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat tggtaacatc
    12001 tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa aatgaagttt
    12061 tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac ccaaggattg
    12121 gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa taatttgttc
    12181 tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg aagcaagtcg
    12241 gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg cagattcgat
    12301 gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc gctgacggaa
    12361 taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat agattgcttc
    12421 tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacgatcttc
    12481 ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt taaatgaatc
    12541 aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag acactttttt
    12601 tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac taaaagggaa
    12661 ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc ggagcaatgg
    12721 gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg atcgatcgtt
    12781 ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt aacggtaaag
    12841 aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc aacgaacacg
    12901 tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt tgttcgatcc
    12961 aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta ggtcatgaag
    13021 atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg atgtggacag
    13081 ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc gaactagaga
    13141 acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa
    13201 atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa gcttgtgtaa
    13261 atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa ctaggcgcgc
    13321 aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct atcgcagcaa
    13381 aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct acttatgatc
    13441 ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt
    13501 tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa tatgatattg
    13561 ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg
    13621 cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct ctttttttaa
    13681 gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa ttgtgcttgt
    13741 ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca acagaggaga
    13801 ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt tttgcccaat
    13861 tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt gtatgcagaa
    13921 attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca ggacgataaa
    13981 aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa tacatggatt
    14041 caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga atcccgcgcg
    14101 attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt tcattccctc
    14161 gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc tgcttatagc
    14221 tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc gtctttggtt
    14281 cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc agcccaagtt
    14341 cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca tcttgtgcct
    14401 gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac acaatttttg
    14461 cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa tctaaaaaaa
    14521 cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc gactgtagta
    14581 aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa agtgatttat
    14641 tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt tctctgtcac
    14701 tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc ctatatttgt
    14761 atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct attaaaattc
    14821 gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt agtcattgtt
    14881 aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga aagaggtcat
    14941 ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc atgttagctt
    15001 taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt gggatcgccg
    15061 gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc gtgatgaaag
    15121 gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct ggtttgatac
    15181 gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg tctatgtgga
    15241 ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg tggttcccgc
    15301 acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc atcaatcttt
    15361 attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa atcagtgtca
    15421 ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg ggcacaggcg
    15481 ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat ggtatgaagg
    15541 gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa ctgattgcta
    15601 tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc aaaggtgtca
    15661 tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt ctttttcctt
    15721 ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa aaaatgaata
    15781 tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc tcatctatca
    15841 attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat cgaaaaggtt
    15901 cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct gttttatttt
    15961 gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac aaattatttg
    16021 gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt atcagtgcgg
    16081 catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc agcttttttg
    16141 cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc attggggtaa
    16201 ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa aagaatgatg
    16261 cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta tgtggaatag
    16321 atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct ttttccgaat
    16381 aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca aaataattga
    16441 ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag tttgttttaa
    16501 atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt tatgtttggt
    16561 atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac acgagtattc
    16621 atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat gtggaaaatg
    16681 tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt ttttgccggt
    16741 tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat gaaggccatg
    16801 attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa agatcctgaa
    16861 gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga caaaatgaaa
    16921 aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga ggatgttcca
    16981 gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg aataaggggt
    17041 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctccta
    17101 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattaaga
    17161 ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa atagttcctc
    17221 atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc gtttatcgag
    17281 aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa atggctttcc
    17341 acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttattcg
    17401 taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta ttgttctaat
    17461 tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat tccccaacct
    17521 gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac gaatggcaag
    17581 taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat tccccaaggc
    17641 actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac agttggatca
    17701 aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac agataggaat
    17761 tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa aatcaaattt
    17821 ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa gccaaattcg
    17881 atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg catcatactt
    17941 tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt tgaaacaaac
    18001 cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg aataaaaatc
    18061 attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac ctttttccat
    18121 gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt aaagtttgct
    18181 tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact tgtgtgtcct
    18241 aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca catcatatgc
    18301 ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt tgattcggat
    18361 aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac aaccagcgct
    18421 ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt tgacataaca
    18481 gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac agaagggaca
    18541 aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat gatccctgct
    18601 tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg gatgattcct
    18661 ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct ttttatcact
    18721 gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga gacctttaat
    18781 ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt tgcttctaaa
    18841 aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt tcttatgttc
    18901 taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc tcctttataa
    18961 aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac cttaattaaa
    19021 aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc catgtaattc
    19081 gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa cacctaaccg
    19141 ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat ttgtcaatga
    19201 aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac acacaagttt
    19261 attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct
    19321 tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt
    19381 agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat
    19441 tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt
    19501 atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct
    19561 atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa
    19621 ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc
    19681 gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt
    19741 tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca
    19801 gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt
    19861 agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca
    19921 ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct
    19981 aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca
    20041 tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct
    20101 tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact
    20161 atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata
    20221 cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata
    20281 taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct
    20341 ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga
    20401 tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc
    20461 ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt
    20521 tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg
    20581 attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg
    20641 ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact
    20701 ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta
    20761 ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca
    20821 gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt
    20881 gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt
    20941 gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa
    21001 gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt
    21061 ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc
    21121 gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa
    21181 ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca
    21241 ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta
    21301 caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc
    21361 attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg
    21421 acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa
    21481 tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc
    21541 catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg
    21601 caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc
    21661 tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa
    21721 aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc
    21781 aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg
    21841 aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt
    21901 agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc
    21961 tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa
    22021 ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca
    22081 ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga
    22141 tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac
    22201 tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt
    22261 gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta
    22321 attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt
    22381 ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag
    22441 gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg
    22501 ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta
    22561 aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat
    22621 caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg
    22681 cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt
    22741 cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt
    22801 ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat
    22861 tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga
    22921 gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat
    22981 tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat
    23041 cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt
    23101 tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag
    23161 cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct
    23221 catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat
    23281 atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt
    23341 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
    23401 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa
    23461 actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc
    23521 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acagttccga
    23581 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
    23641 cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt ttcaacgaaa
    23701 tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat
    23761 tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc aataacagtt
    23821 cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac ggtataattt
    23881 cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt
    23941 gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc tgttcgttcc
    24001 aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc tccgaatgta
    24061 aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc tcttttcgta
    24121 acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc caatatttct
    24181 tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa atattcttca
    24241 cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac taaaatatca
    24301 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg gatatggata
    24361 tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt gtcaactaac
    24421 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
    24481 cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc cttctcgttt
    24541 ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca agcgccagat
    24601 aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc atcaagccta
    24661 attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct aatgtagtga
    24721 taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt gtacgcagtg
    24781 catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca tggaataatg
    24841 cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc actacagcag
    24901 gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa cttgttggac
    24961 caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc tcgtccaaat
    25021 attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag atcagattca
    25081 aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt gcaatcagca
    25141 cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc atgatattcc
    25201 caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa
    25261 ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa ataccgatat
    25321 cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg acagtatgac
    25381 catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat tttacgaata
    25441 cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag attttctcac
    25501 cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt gtaactaatt
    25561 cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct ttcaacgctt
    25621 gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa accatgtctg
    25681 actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt gcgacaccaa
    25741 tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct tcttttcgtt
    25801 cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg ttcagcattt
    25861 cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca accacaacac
    25921 tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa ttgatcttat
    25981 ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa tttttcagcc
    26041 ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct tcatttcctt
    26101 cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc gttttatccg
    26161 tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg atcactgttc
    26221 cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc attggaagca
    26281 tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct agccaatccc
    26341 cttttgtaag cccattgatg acgataactg ctggagccat caatgacata aatttgatca
    26401 gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt ttttcagata
    26461 gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg attccttccg
    26521 cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt gtttcgcttt
    26581 gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc cctgtcaatg
    26641 ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct ggtatcatat
    26701 cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga atctctgtgt
    26761 actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt ttttcagccg
    26821 cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata accatagtga
    26881 agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca tattccgtga
    26941 tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca
    27001 atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca ccgtaatttt
    27061 ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg aatagttcaa
    27121 ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta tctttctttt
    27181 tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt
    27241 tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag atagataata
    27301 gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt tgttctcatc
    27361 tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag ttatttttcc
    27421 aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca aaaaaacctc
    27481 tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc tcagatctca
    27541 tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag ctaccttatg
    27601 aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca
    27661 gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt ccttcgttaa
    27721 tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata ctttttttaa
    27781 aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc cacctcccgc
    27841 tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt gttattatca
    27901 agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt gaagcaaagg
    27961 gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca aaagaacatt
    28021 atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg acttccggtc
    28081 ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggttg
    28141 gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac gcgctgccgg
    28201 ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc
    28261 gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat
    28321 tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt ctctaaggac
    28381 ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg gtagtctttc
    28441 gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc catcaatgtt
    28501 tcatagatca ctgtacttct ttctttatgt gataagctcc gtttcatatc ttcattcaca
    28561 ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt cattaaaaaa
    28621 gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct ttttttgatt
    28681 ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc agctagaata
    28741 gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat gattcggcat
    28801 tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac catgtgaaga
    28861 ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt attgctgatc
    28921 tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct ttttttcttt
    28981 aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag tgtcatgttc
    29041 attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga atttgtctca
    29101 atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg tgtcaacttc
    29161 ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct cctttttaat
    29221 ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca cgaaaaaaat
    29281 aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt tgtctaacta
    29341 ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc tgttcttgtt
    29401 actggatata agttggttgt tgttttatta ccattttttg aaaagacact tgtcgaatgt
    29461 tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata gtcatatttt
    29521 ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt ttcctgattc
    29581 agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga gatttcctca
    29641 tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta agcttttcct
    29701 cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac tacttgatca
    29761 tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc gattcccaat
    29821 agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt gactgtccct
    29881 ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat
    29941 tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt atctgtattc
    30001 gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat accagatttt
    30061 ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt tcgaacataa
    30121 ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt gtcatccgta
    30181 taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc
    30241 tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata acctaacgtt
    30301 tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa gtttttatat
    30361 acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct tgtatagcct
    30421 tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc aagttctgtg
    30481 aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc agcatcactt
    30541 gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg
    30601 aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac
    30661 tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat cccaaatgtt
    30721 tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc aatcccatta
    30781 ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc ataaatagta
    30841 tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg ctgcagcaat
    30901 tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc tgaaagacag
    30961 gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat tttcttgacc
    31021 ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat gacatcatgg
    31081 ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga aaagcctaat
    31141 acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt caagtacaac
    31201 aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt tggcttttta
    31261 atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat ccacaagata
    31321 aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc aatgatcgtc
    31381 ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg gcacaatttt
    31441 ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt ttgctcctga
    31501 aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt tctaaagatt
    31561 attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga
    31621 gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt ttctcggtat
    31681 tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt cttgatgcag
    31741 gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc tcggccacaa
    31801 gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag
    31861 ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac cctcggtatt
    31921 agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa aagcttgcat
    31981 ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga aattgctcct
    32041 tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg
    32101 accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct
    32161 ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc cataggattg
    32221 atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt cacttcccgt
    32281 tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt ttttttgaga
    32341 agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta aacgatcggc
    32401 gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat ttttcaaacg
    32461 ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt ctttaaattg
    32521 atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt gattttcttc
    32581 ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag ttattttata
    32641 ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt ctttgcttgc
    32701 tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg aaaatgctgc
    32761 ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt
    32821 cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac aaaaaatgaa
    32881 atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt tcacaaaatg
    32941 gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc ctcaaatccg
    33001 tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct ttttccatct
    33061 tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt ccacgaccag
    33121 ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg aatggcttga
    33181 tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg gatcatgcca
    33241 tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga aataaagcag
    33301 tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc ttgttcatag
    33361 atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt gtcgaaataa
    33421 tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt tacccggcgt
    33481 ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac cgtcaaatcc
    33541 tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa ttcacttgga
    33601 aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc tgctatttgc
    33661 gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt cagattcaag
    33721 atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc taacgctgaa
    33781 gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc ttctggataa
    33841 tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg agtaaaccat
    33901 ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc ataggcttca
    33961 accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat atcgaaatga
    34021 tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata acgatgatag
    34081 aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg gctccattgg
    34141 atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc ctcttgatac
    34201 ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt ttgcggttct
    34261 tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat ccttccttct
    34321 gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg atattttttc
    34381 atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta tgatattttc
    34441 catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta acagctgttc
    34501 tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg gaaggatact
    34561 cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc cgattctgac
    34621 accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc tgacaaaaat
    34681 agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt ttctagaata
    34741 gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt ctttggctcc
    34801 tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc cgattttttt
    34861 atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat cattcactaa
    34921 tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg gaatactcat
    34981 ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa tcagcccatc
    35041 gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct gatcggcaaa
    35101 cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc ctgagacaac
    35161 ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt
    35221 attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc
    35281 tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg aaactgttcc
    35341 cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt ctcactcctg
    35401 ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat tctttgcata
    35461 gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac ttcctctcac
    35521 tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc atttttttaa
    35581 aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg cctttttaaa
    35641 attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc ttccatccgt
    35701 ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg gaagtatttt
    35761 gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga ctggctgtga
    35821 tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg tcttaaaagc
    35881 ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag atatggattt
    35941 atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca cgtatcagac
    36001 acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag atatcaagat
    36061 cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag aaacaacagt
    36121 catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca tctacagtct
    36181 atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta tagtaaagaa
    36241 gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat tgtacatgta
    36301 ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc atatgcgaca
    36361 actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct gttcaaaatt
    36421 atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc tgatttttat
    36481 gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa acagtggatc
    36541 atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg aatataaaat
    36601 aaccaaacga tagaaaggct tattctacat cacgtagtaa tttttctttc ccgtcgtttg
    36661 gttttttatt tttagcctaa ttcttgacct tttgcatgct ggttatccgt aaaatatttt
    36721 ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacggat cacctttttt
    36781 gcatcttctg gattttctac aaaatcgaat ttatccccat ccacttgtat tttaggacat
    36841 acatcaaaat cttcatacca ttgattatag cgggtgttca acgtttcata ataggtatat
    36901 aacgtctcat cgctttctag ctgttcatag tcacgcccac gttttttgat acgttctagc
    36961 atcgtgtcca aagaaacttt gatatggacc atcaaatcag gccgtttttt tggagcaaca
    37021 tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac ttcgatatcc
    37081 gttacccgac ctaaatcagc gtttagatgg aacaacaggc tatcttcata gatggatcgg
    37141 tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg cttgttcaaa
    37201 aagaagattt gcaataaaaa agcgtactgc tcaggatttg aataaaagag cggcaatact
    37261 tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc aagcatatcc
    37321 gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga catacaaccc
    37381 ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatgtttc aaaatcatat
    37441 tcacgtacag cttggtctga ttttaaatac gtatggagac tttgtgtaat catcaagttc
    37501 gtaggtaccg tctcttcttg tacaatgatc atcggttttt tcaacatagt tgccacacct
    37561 aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat agcaacaaca
    37621 aattctgcat ttgtcagttc ttccatatct ttttcgtata cttcctgcgc ccatccagcg
    37681 ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact tgtgactgtt
    37741 ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc ctctgaaaaa
    37801 aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat aactgatttt
    37861 taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa aagattactt
    37921 gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat aattgttgca
    37981 agccgctgta ataaccgcca atattcaata ttccgattgg cttgtcgggc gtcagaccga
    38041 ttgctatcca agaaagaagt tgtgagaatt cctccaatgt acctagacct ccagggaaaa
    38101 cgaggacagc atctcccttt tcaaataata atacctttct ttcatcgatc gtttctgtag
    38161 gaataaatgt agtaacatct tttccaggca attcagtttc aaaaagccca ttcggataga
    38221 tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc ataatcccgg
    38281 agtatgaacc accgtaaacc agttcgattt cttcttgtgc catgtatttt cctaacgttt
    38341 gcgcaataaa tttgtaactt tctttattac caaaacggga accgcaaaaa acagtaagtt
    38401 tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg tttttttaaa
    38461 ataaaagact agattttgta gttttttagt ggtatatagg atagacgcca ctatatatag
    38521 tatttagaaa aaaatcattt ttcagccatt ttatagataa aacaaaaata aataacgtgt
    38581 tttcatcgat atgaaatgac ttacttatag aagatactgg gatttcgtcc tatatgccaa
    38641 taacgtgtat tattttaaac aacaaatact tgtttctttt attaacgaaa aaatcatttt
    38701 gttctcgtgt tatgtttcac tgataaatga tgtttctcta aataaaaaag ccgacgaact
    38761 aaacgatcaa aatttgatca tttttgttca tcagcttctc ctaaaaatct ttttgttaac
    38821 gataaatagc tggatttgta aaaatatcac ttgaatcgtc actgctggaa gaaaattcag
    38881 tgataaccgc acctgttgcg cctgcctgga actagtgttt gatattgggc tcgaacgtgt
    38941 actgatctct agcatgaaga ataatttctt tttttactgt ataataactt tctttccttt
    39001 ttggaatagt acaggaaagt ttttttgttt cctttccttc gacataaggt ataccagacc
    39061 ctttcgacaa cgaaaggttc tcatttttcc ttcatcgtca tttcaaggag ggtgccgatg
    39121 ctctggacag tttcgatcgc tcctaaacca aaatctgtat attcgattct ttctaactct
    39181 gattctttca taccattcct gtctcaaggt acatctattg tacttttttc ttgtaatcca
    39241 ttctgcctct cctaactaat tgtcaaatca tcccaagata tgtcttcttc ctctttttct
    39301 tcttggatga tttctgcatt cggatctact ggttttttca ataataaaat ggttattcct
    39361 gtgacaaaag aaccagctaa caatgcacca atgaatgccc atggtctggt catcgtagcg
    39421 actgcaaaca ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat gcttaatccg
    39481 ccacctactg tcccaccgac tcccgttgct atgacattgc gtgccaagtc gttcaatacg
    39541 atagggaaac atccctctgt gatctgacag atccccatag gaaaagcggt cttcaatgta
    39601 tcgatttctt gacgattgag tatattttta cgaatgattt tccctaagaa ataagcgatc
    39661 gtcaagccga atggggttac catcgaagct aaaatcaata cagtgattgg ttcatagatt
    39721 ccttctgaaa atcgtgccac acccccttat ccagaataaa tggtaatcca gaaacagccg
    39781 tgtatctgcg gtaataagcc taaccaaacg aaaaatatga gaagctattt ttgtttggct
    39841 gttcttatta cttgatgcag ggcagctttt tcacaacctc tttttttatt tatgtttcat
    39901 aatcaatatg gtaaggatgc agctccgtat ggtagaccgc ctccgaatat tcaatcattt
    39961 ccccaatttg cgtagacgat ttccgtatcc gtttgatgcc gatctgtgcg tctgttttca
    40021 gaattccttg ttcttcttct gttaaaaata ctgccgagaa atcatcagaa aatgagtcaa
    40081 tagtatatcc ttttttcgtc agcaaacgat acaaagattc tttgccaaat tcttctctgc
    40141 tgaattgcat catttcttgt ggaagataat gaacaaaata aatataaggc tgattgttca
    40201 aataatataa gcgcttaaaa caaacagctt gctcaccgaa aaaggaaaac aaaggatctt
    40261 ccttctttat atggaccaat tgcaaatcca gcgtttcttt ccggacttca tatcgggagt
    40321 tctccaagat ttgcgtaaag cttgccgctt tggataatcg attgtaagga cgattgctta
    40381 gaaccgtcgt cccacgacca cttttctttg cgacatattc atctgatgcc agcagttcga
    40441 ttgcctttcg gacagtgatt ttgctgactt gaaacagttc ttctaattca ctttctgtcg
    40501 gcaacatcga accgactgga tattttcctg agaaaatatc ttctttaatt ttatctgcga
    40561 cttccaagta tagtacacgt gtccttttca taacgtcacc ctttgcttta ctattactaa
    40621 aagaatagca cataaaaaat aaaaaaagaa cacaaatcac atgtgagaag tgtgttcttt
    40681 ttcattcagc tgctttaatt acaagtttac gccatttgag gcaattactt ctttatacca
    40741 atcaaaagat tttttctttg atcgattcag tgtcccattt ccttcatcgt ctagatccac
    40801 atagataaag ccgtaacgtt tactcatttc tcctgttgat gcactgacaa gatcgataca
    40861 tccccaaggc gtatagccca tcaaatctac accgtctgct actgcattag ccatcgcttc
    40921 gatatgacga cgtaaataat cgatccgata atcatcaata atcgtgccat cctcttccat
    40981 atgatcaatg gctccaagac cgttttcaac aatgaataat ggtttctggt aacgattgta
    41041 taattcattc aatgcgattc tcagcccttc tggatcgatc tgccagcccc attcacttgc
    41101 ctcaagaaac ggatttttta ctcctcccag caagttccct tctgaagttg ctgcttgtgg
    41161 atcagtctca ttgattgccg tagacatata ataactaaat ccgatgtagt ccactggata
    41221 ttcttttagc aactggaagt ctttttccgt ttgctctaat ttttccggat cgactccata
    41281 ttttttatgg gtacgcgctg tgtatgcagg gtattctcca cgtacttgga catccgtgca
    41341 gaaaaaattg aattcttggt tttggatcat tgttgctgcc tgattgactg gattggcatc
    41401 gatgctgtaa gttgttgcat agataatcat gcagccgatt tggatatccg aacgcaattc
    41461 gtggccgatt tttacggctt tgctacttgc aacgaattga ttatgccatg cttggaaaat
    41521 attttggtac tctcctgctc cattgctttt tactaatcct tgacttaaag ctggaaagtg
    41581 aaaggccgaa ttgatttcat tgaaagtcat ccaatatttg actttactgc tgtatctctc
    41641 taatactgtc tgagcaaatc gttcatagaa gtcgatcagt tttcgatttt tccagccacc
    41701 gtattctttt gccaaatgaa gaggcatttc gtaatgagaa atcgtgatca caggttcaat
    41761 atcatatttt aagcactcat caatcagctg atcataaaat tctaatcccg cttcgttagg
    41821 agtaccttcg tctccatttg gaaaaattcg tgtccaggca atcgaaaaac gataacattt
    41881 gaatcccatt tccgcaaaca aggcaagatc ttctttgaaa cgatcgtaat gatcgattcc
    41941 tcgatgattc gggtaaatat atttttcttg atcaatcgtc cagtcaaatt cttcactgcc
    42001 gataatcgca aagcgctgct tacctccagg catagcatct gcaacggata gtccttttcc
    42061 gtctcgatca taagcccctt ctaattgatt tggcagcagt tgctccaccc cataaaaatt
    42121 tttctgaaaa acgtgattgc atcctgattt cctccttatt ttacaattgc cagaacatct
    42181 tcgtttgcaa taatctcttt ttggtctaat tcaagtatat cggcaaatgc atcagtattc
    42241 gtaatgacaa ctggagtgat tacgctatag ccagctgctc taattgcttc gatgtcaaaa
    42301 cgaatcaata aatctcctgc tttgacgaga tccccttgtt tgactaagat ctcaaagcca
    42361 ttcccattta attctactgt atccatcccg atatgaatca aaatctccgc tccgtcagtc
    42421 gttgtgatcc ctacagcatg tccagttgga aaaatagtcg tgatctctcc gtctgcaggt
    42481 gcaactagtt ctcctacttc tggatcaata gcgatacctt ttcccatcac gccagaagaa
    42541 aaaacttggt ctggtacttc actcaatggc agtactttac ccgttaaagg actttttaag
    42601 gtatgccgtg ctgaagtaat cggttctcct gcattcgcat gtttgttttc taattgattg
    42661 tcttgtgttt gttcgtcaaa tcctaaaatc aatgtaccta cgaatgcaag aacaaaagca
    42721 attcctgtag ctattactgc tactgtcaca tttgactcta caccatccac cgtattaatg
    42781 aaagtcggaa tcgtcaataa gctgaccaaa ctgctggaga aagcttttac tccggaaaat
    42841 ccgataatag ctccaccaat acctccagaa atacaagcgg caatgaatgg tttcttcaaa
    42901 ggtaatgtca caccatacac agttggttca gtgattccaa ataacgaagt cactgtagaa
    42961 gaaatcccta atgctcgaag tttcgtatct tttgttcgca atgctacagc taaagcagcc
    43021 ccaccttgtg ccaagatggc tggaagaagc attggcatca atacatcaaa cccatattgt
    43081 tcgatattca aaaacatgat cggtacgaat ccccaatgca tgccgaacat cacgaatact
    43141 tgccataaac ttcccatgat tagacctgct acgattggac taaatccata aatcgagttg
    43201 aagagaccgc ctaaagcatt accggcaacc gtcccgattg gtcctaaagc aagaaatgtt
    43261 aatggaacca tcactactaa aacgacaagc ggaactaaaa tcatttgcag aaattgcgga
    43321 atcacttttt ttacgaatgg ctctaattta ctttgaaccc atacggctag aatgattggt
    43381 atgacagaag aagtataacc agatggtgaa agaatcactg ggatcccaaa gaaactgata
    43441 gcttctcccg caccagcaac tgctgtgata gctggataca caagtgccat agcaattgct
    43501 actgctaaaa actggttggc tttgaacttt ttagctgctg taaaagcaag catgacaggt
    43561 aagaaggtaa agacaccatc tgctgcagca aacaatattt gataagtacc gctatcagct
    43621 gtcagccagc ccatcaccgt tgccaatgat aagaatcctt tcaatacccc ggcggctgcc
    43681 atcgccccta gaaaaggtgt aaatatacta gagataatat cgatcagacg attcaaaata
    43741 tttttggact cttcttttcc atcctccgaa actgcaagac cttcctcatc cacgatcgat
    43801 tgataaacat ctgctacatt actgccaatc actacttgat actgaccgcc actttgtacg
    43861 acttggatca cgtccgggtc ttcttttaac ttttgcgtat ctgctaaaga ttcatctttt
    43921 aatctgaatc gcaaccgcgt agcacaatgt actaaacttt tgacattctt ttgtcctcct
    43981 accgcttccc atacacgacg accaactgct tgattatcca ttgttttttc cttctttctt
    44041 ttttatttaa aaaataaaaa cccagattga aaaaaacagt cacaaacatt cgtgaaccta
    44101 ttttttcaat ccaggttttg cctgccatac agtaacaatc cttatgattg gatgcttttt
    44161 ttctcactta ctactctttg aatatgaatc gttaaataaa tctgctctgc gatccccatt
    44221 tcctgttttt gcgtttgtcg aagatactca ttgatccgtt cgacacaatg aaacgctttt
    44281 ggatactttc cttgaactaa tgcgtagaga aattcatctt gctcgtcaga tccttcccct
    44341 ttgagataac gctgggcaaa aaactgcaga tgagtaatga tacgctgata attcaaagat
    44401 tcttcatcga acacgatgcc gaagaattta ctgatgatac tcagtatatc tcttaccatt
    44461 gtggtagcgg tcatcgtcac ttgcatgtct tcgtttcctt gttggctatt cacaaaatga
    44521 aaggcaataa aaccggcttc atcctcagaa agttccacac ccgtcttttg tttcacgaac
    44581 aaaagagcct ctttcgctgc ttgatattct ttcggataaa atttttttgt ttcaaataat
    44641 aatggattag gcagttggat attttctttt gctcgtgtga tcgcataatg aagatgatcg
    44701 gtcaatgtca gataaatatt aggcgaaaat tcaactttta gtttatcttc agccagttcg
    44761 actacttgtt tgacccattc gatctcctct gccggaatat cagccatcag ctgctggaat
    44821 tggtttttag caaaagaatc tcttaaacaa attctttttg gaccgcttct ggcgaaatct
    44881 cgtccccaat ctttttttga aaagctagtc cttttcccat acaaacgact tcttccccga
    44941 atccattttt cgaaatgacg acattgttat tcaaaatttt atcaattttc attttgcttc
    45001 gccctctcaa gaaaaataaa aaaacctaga attcgcctac tgtttcttag ctgaattctg
    45061 gttttgcctg ccgtacagta acaatccatt tcttattcaa aatcatcata ccatcgaatt
    45121 tttatttatg caagcctttt catataaaaa ataaaaaaac tggaacacaa gtctatctag
    45181 aaaacaacca aatcagagat gcacctcttt gcgtttctct ttttttcagc aaattggttt
    45241 ttccttatcg attcatatcc tttggctgtt tttaatctgt aacgacttat gtcccagtat
    45301 cctaaaatgt ttttgtctct agattgaaag cagatctcat atcatctgtc gcatctttta
    45361 tcgatgtaac actttccagt tctccttgta caactagtcg cgtgacggcg tccatgtcgc
    45421 cacaagtaat tagtgtgaca atttcttttc cctcgacttc attcagtaat tgggtatctg
    45481 taggagcaac tttttcttta aaaaatgttt tgtatgtata cacattttct aaatctgtca
    45541 aatagatctt ggcacccatc tctaaatttt ctagcggtgt aaacagcaat tcagggttcg
    45601 ttgcacgatg gcttgctaag gcataatttc cttttcccat ttcttgatct ggagataatg
    45661 ttcctgctcc gtaaagtagt gacgtattat ccaagccttt gaaaaccggt aaattgattg
    45721 aaactgaagg gactgcgata ctagcaatca ctggtagatc tgttcctctc atttgcgacc
    45781 tcatgacccc ttctgtcgtc attggttcta ccgcatcaaa gtcaaaactg acatctttat
    45841 cgttattctt tttcagatct tcttttgtta cattcgctat agcatattta tctcctgtct
    45901 ctctgacaaa ataatccttg atctgttcat tgaaaatcaa agctagtcct actaataaca
    45961 agaggaacaa gaggatattg actagccatc tttttgcttt tccttttttt ttcattttct
    46021 ctccctccag actcatttta ctgtttttct tttgctcttt ccaacagccg aatgacacat
    46081 ttctacaact ctgtgtattt ttttcgtaca cctgcgcttt tttctacagg atatttcaaa
    46141 ttatttttga tcaatttttc ttcaatgatc tcatctagat ccaggtgaag gttgtctgcc
    46201 atcatgtatg aatagatcaa tacatctgcc agttcttctt tgattcgttc taattgtgtt
    46261 tcttttactt cttccggcgt tttccattga aaaagttcta acaattcaga tgcttccaaa
    46321 gaaatagaga tagccaaatc tttttcgtta tgaaattgtc gccaattacg ttcatctcta
    46381 aactggttga ctttttccat actattcatt tgcttgcctt cttccaatca cttcttactt
    46441 gattaatcat accatttttt tacataaaaa atagatgatg cttttaaaaa aactcctgtt
    46501 ttcttttatt taaaataaat gtcatagaaa atcaataatt taggtagcca ccatgtgtat
    46561 gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc gatacgttct tcttcccaaa
    46621 cagacaacgc tactagaatc tctcttaatg attttccttc ttcggtgagc gagtactcta
    46681 ctttaggcgg gacttggtta taggcttttc gatggatact ttcatcttgt tctaactctc
    46741 ttaactgctg ggtcagcatt ttttttgtga tcgtagggat ttgcttgctt ctccacgcct
    46801 aaaataagcg atattgctct tccctttatc tggtgtaagc aaagcagtta gcattttgac
    46861 gatcgtgggt tccgcttttc acttcttatc tacttagtat catgaagtat actttcgtac
    46921 taatactaag tagcggaagt gtcaactact ttgtttaact acatagcaaa agaaacagaa
    46981 gggtgacaac ggtcatcacg cttctgtttc ttttgaatat gctgtgttgt tgtctctatc
    47041 tccgtgtaga ctttcctcaa acttatcctt tgacagtaag tcagtaggct gatttgctct
    47101 ttctagatgc cagatacatt tctacaaaaa aatagtgatt ctgttttgaa aattcgattt
    47161 ttagttctgc attttcgtaa acatccaaca tttttttcgc actgtataga cctaacccgc
    47221 catttttttt agaggttttt cctcttttga ccatctccga tagcgtcaaa ttcgttggat
    47281 ttgccttgtt cgctatacta aaacgtgatc cctctttttc ttccagcaga ttgatcgtaa
    47341 tcggactctg atcggctaca cgatgatcga atgcgttaga aataaaaata gataaacagc
    47401 gaataaaaac aaccagatca atcgggattg ttttcacttc gacaggaata actacttgga
    47461 tcgggatttt ctccgattct gctttttcta gaaattttaa aaagacaccc ctgacagctg
    47521 gggatacaat atttgttagc tgttgtattt gcggtttttc aaaaatagct gcagaatgat
    47581 cttctaatat ttctaaaaat tccaacgttt cttttttatt ttcctgaaga gtaattttta
    47641 atgaaaacag tagactttta taatcatgtt tgaattcctc tagatgatgg taatattctt
    47701 catttatttg atacagttga agcgattcct gatgttgtct ttcttttact tggaaactga
    47761 tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac tatcgacaaa agagatagaa
    47821 gcagataatt agatgagcgg aagtgaacac ttcccaatac caagtaaatc acgccccagc
    47881 ttaataaaat acaaatacct ccaaaatagt gttcagttgg ataagtaaaa agcaagttga
    47941 gaaatctcgt ctgtttgtct atacttttta ctaatagata gaaaataaat gcgataagta
    48001 gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa ataatccatc agatcaaaaa
    48061 ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt actgatcaaa cagctgataa
    48121 acaggttttt cgtagtaaaa taaaaaagaa acatcaataa taagccaact aggtaaggaa
    48181 agctcacttt agggatagga agtaaaagtg tcaccaccat caaagctata caaccaaagc
    48241 tgttttttac tagcagttgt ttcgaaaaga ataaatagat acctaaatct aaaatcaaag
    48301 taaaggtcaa ataaagtgtc actgttgcga ccatcttatt cctccattgt tttgtttcaa
    48361 aatgtacaca tattttttta ataggagcct gcatatgaaa aagtatttta tcatagaaga
    48421 taaccagttt cacagcgaat ggatcatcag agaattggtc aatcatcata tcgagaatat
    48481 cgtgcagctc acttctatct ctcaaatcaa tcagttgatc cagttgggca ctattgataa
    48541 tcacgatgtt ttctttatcg atatcgactt gaagcaatca ctcacaggta ttgatgtcgc
    48601 cgaacaaata cgtaaaaaaa atgcacatag taatattgtt tttttcaccg cttatacgaa
    48661 ttttgcagta gagatcatca atcggaaaat ccttccgatt ggatatatca gaaaagacag
    48721 gaatttatct gaacaaattc gtcaagtgct agaacagatc aattggaaag aacagcagat
    48781 cgtaaatgtc gataaaatct ttattgatca aaaaggtgga agtctcgtcc tgtatcctcg
    48841 ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac caagttatgg tcaaaacaac
    48901 tacagaagat aaattagtga atacttcttt aaaacaattg aagacagagt tagaaaagta
    48961 tgcttacttt ttacttttaa aatcttactg tatcaactta tcgcaattgc agaaaattga
    49021 ttctgctcat tatgtgttag aatttttcaa cggtgactcc ttactcgttg gtcgaaagat
    49081 ctttgaaaag acaaaggaac gtttccatga cttccaaaaa actgcttcga gttgatgaat
    49141 aagagctgag acgtaagcat agcagatttc agggagtggg ccttgaatcg gctccactcc
    49201 ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag gagcatctgt taacgaccat
    49261 gtgaatgttg aaacgtagtt gtctacagca taattggata caggaacaaa taattccact
    49321 gattgtctcc cttgttccac attctctcct aatgaataaa tccatgtgcc aatcccttca
    49381 tttgctcctg cagttactag atctactgcc tgatctgctg ggatttcgat tccttcagcg
    49441 ccagttccag aaacggtaga tggcattcca ctgctattag ttgcgctatt tcgaatcacg
    49501 gcgtttttga atttcacctt tgaaccagtt agtttattcg tatcagatgt tgtaccaata
    49561 aattctgttc gtttgactga taatttccaa ccggcataat ttccgctttt atccgttact
    49621 tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt ccgttccgct ttgtaatgca
    49681 gcatatactg tttttgctgt acctaacttg attgttccaa aatcaagatc tggagcttgg
    49741 tcaatcgtca gtaatccgga tgttccctga tttggttcgt caggtgtaaa aggagtatca
    49801 ggatcatttg gatcaacagg aggaactgga acaacgtctt caggatttgc agcatctggt
    49861 ttggtcacag aaatcgttcc ttctgtagtg gctgagctct tattctcagc aaaaacactt
    49921 tgtgtacata ataatagact tccagctaga acggtactta atgcaattgt ttttttcatt
    49981 tctttttcct tctttctttt aaaataaata atccagcaaa taagccaata agaatgactc
    50041 caacaagtat cagccatcgc cagtcaaatg tttctggctg attttttaaa tcaatatcct
    50101 tttcattcaa ttggtccgct gcatcatcag ttatttcaaa atcggtatgg agattccagc
    50161 gtttttcttt ccaagcaccc ttcacaaccg cttgataatg tcccggagct aatttttttc
    50221 cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc tacatctatt ttttcttttt
    50281 ttattgaaag gtaaggctct tttgtttctt ttttgtagat ttcaaccgat gtagtcaagc
    50341 cgaacatata agaagcattt ggattttgaa gattcgcctt aatcacattt cttccatcgc
    50401 tttgatccgc ataagctttt tttaatttca aatctgggga agactctttt tcgctatttc
    50461 aaatcaagat tgctttcact tgagaaaact gattgacgac accaactgta cttttgttag
    50521 aaacgttctc ttttttttct tgaaacgtga tccctcctgc gaccacacca gccagctctt
    50581 tttctggcgc tttcagtgaa agtgtctcag tgaccgcact ttgaggagga acagttattt
    50641 ctcccactgt ttttgctaag tttttcatat cataacttaa tgtttcatca aatttacttt
    50701 ttgattctcc atattcgatc acaccgtttg aattcgtcgt agcggagtgg atcaatgtct
    50761 caactgttac tgatttatct gttcggttaa agaccgtgta ggggatttcc tgtgttttcc
    50821 caggctccat ccttacattt atatatgttt tgttcttatc aatctgatta gccggtaatt
    50881 caaaatctac cgcaaaattg aattcggagg catggacagg tgtctgccaa aggatgaatc
    50941 caaggagaag gatcataagt aaagagcagt tgtttcgatg acttttattc catattcgat
    51001 tcatcaatca tcctccttga tctttattct gtcggcgcat ctgctaatgt ccatgttatc
    51061 tctgctgagt aattaccagc tatctgattt tctttggtta agacaagttt aaacgcccga
    51121 ccgttattta aatattctga ataatctgct tgcacattcg ttgaattttg tcctctgaca
    51181 atttccagtt cttcttcatt cactaattga tactcctctc ctttcttgaa atataggaca
    51241 tcctgcatag aaaatcctgc atcatttgtg aaaggctgag ataattttat cgttaagatc
    51301 cacggatttt tcgtttctcg ctgatcataa atccataatt tttcctgtat atccatttgg
    51361 ccaaatgctt cttgtgtcaa accagttgct ttgatctgac caaaatcgag tttggaaggg
    51421 gcaagaaatg agagaccacc ttctactacg atcttcgtag caggtgcaaa aggcggggca
    51481 tcatgataaa caactggcgt atcacttgga ttgtgattcc caatttcatc gttggtactt
    51541 ggcttgttct gcacaccctt agcagtagct tctcctgctt ggtcgtttaa tacaatttct
    51601 aatacatctc ctgcaaaaag ttgattttct gggatagaaa gaacaaattc ccctgtatca
    51661 tcaatagttg tttctgacaa aacagcttga ttttttttca atgaaaccaa ggctcctgct
    51721 tcacctgtac ctgtgatcgt gcgagaagct gcattgatca atgtggtact taaattagca
    51781 ggttcaggag gaatcacatc gactgtcgtt ttggcagagt ccagttctgt cacttcttgg
    51841 ccacctctta cttttttagc agcaacaact tgcacttgat cctccgtttt taatctctca
    51901 tcattgggta cttgaacttc gaacaagccg ccttgttcgc cttctcccca tgggttatat
    51961 cctgcccctt cattttcatc tgtttgtcct tgcgtgagag cagtaatttc tacagatgat
    52021 ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta cttcgcctgt ccaagcatct
    52081 cttggctttg cctcgacacc ttcgggaaca acgacatgcc catagatttt tttatctgca
    52141 ttcgttggca ctcttaagga ttcaatcatc gggagctgat tattggcact gattcgatta
    52201 taatttttca ttccggcacc tgtctgagca aattcatttg ttaaacctgc actagggctt
    52261 gatacgaaat tgcctaagtc ggtcccattg atcgataagt ctgtcatatc agaaaaataa
    52321 tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt ttcgccataa agcgatttct
    52381 gaatgggcga tctttaaaga agaatctcca tttccttgga atacccagcc gcctatgcca
    52441 ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat aggtaggaga ttctaagtcc
    52501 acattcaaca catccccact gatgatgccg gtcttatctt cccctgtttt cccgaccatg
    52561 actaaataag ttttttcacc tgccgtaaaa tttaaatcaa tatctccagt tgtatctaca
    52621 gttgctccgg aatctgcgcg tatatcgata tttgattttt ctccagtgac tgtaaaatca
    52681 gctgaacctg gactttctag aaaaccagaa gcgtcataaa attggatcgc ttgatttcct
    52741 tgattgagtc tgttcacgta agatttgcca tcacctgtat ttcttaccct caatgagcct
    52801 ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac gaactgcgct ggaacgccca
    52861 ctctgcttat cgatgtcgat aactgatccg gctccttcta cgatcaaaga aaggcctttt
    52921 cctacaaaac gcatcccatt gttcccgggt tgatcgtcat ctccagccat tttgaccgag
    52981 atttgtccac cacttgatac atggatttga ctagaatctc cttgcaagcg aaatccgccc
    53041 gtagttgctg tattgatggt gattttccca ccattcgata cagtgacttt cgatcctgaa
    53101 cctctcattt ggatgattcc gcgattgttg tcattggtaa tattgatctt tgtatttacc
    53161 gaaaattcgc ttcccgaacc actgacttca aacatgacat taccaggtag cgtatcgact
    53221 gcctctcctt catcgctaac gccatctgcc cgatcataat caagagagac ctttgaaccg
    53281 cttaagatat ggattcctgg cgctgttttt ccactaccag tctcatctcc tgaatctaga
    53341 aaaccgtaga atttccctag atacggtcca taaagttgac taccattgat cacattcaat
    53401 tgatagcttc tacaaaaata ggctttaccc ggagcggcat tcagtccttc cttcactggt
    53461 tggacatcca acacaccgga aacaccatct aatgtgacag ttgcacgagg agcgtaaatc
    53521 agtcctaatt tggacccatc atacaaatca agtgtcccgg taaattttat ttctcccgta
    53581 aaatgatatc tgcttcccca aaaatcggtc atataagcaa tgataatggc atttccatcg
    53641 atatttggtg tcaatccacc tggatgtgca gctgttcctt taaaattctg gaactcgatc
    53701 gactgaggta tctcattgat ataaatatgg tgtgtgccga aattgtgagc gaatccacca
    53761 aaatcaataa cgatatctcg tttgatctct aattccctgt ctaaagtcaa aggctgaatc
    53821 agtcggatct tagttactga ttcgtctgcg agaagctgcg ccagttcttc tgttgtggca
    53881 gcttcgctgt acccatccgc tgaaacagct cttgccagtg ccgcttcact gctatcagac
    53941 gtttgttcag ctacttctga ggtctcactt gtcttatttt cttcttgcct tttattagta
    54001 gaagcctcac tttgagattc tgtagtttca gaagtaatcg attcttctga aaaagtagtc
    54061 ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg ttttctctac atcggcaaaa
    54121 acggatgtca ccatcaagag agggaagaca aacaaaatcg acgcaatcag cttttttaca
    54181 cattgattca ttctccacca tcctctctga cgttttgaca attttatttt atcgttttaa
    54241 tctatttcca attgtacttt catatttaaa atatgtattt ttatggaaaa atttttctat
    54301 taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga caaaagtctt gtcacagtcc
    54361 cttatcccga acaagcggtg ttctcccgcc aactcctcgg tcacaacctc cattttaaca
    54421 ataaacctgt aaatgtgagg gcaatacttt gattgtcaca ggtaatttat ccccttcatc
    54481 tccatcaaca ttcgtcgtca attcttcctc atcagctaat gacacaacta attgctcaca
    54541 agtaaggtag gctagatttt tcgtagattc atctactcct tttaataggt ctggcaatgt
    54601 tttaagcgta tcgatcaaag aagaatcttt taaataaacc aaatggagct taccatcatc
    54661 cacttttgca tttggaagta atgtttcaaa accgccgact gagttagtta gtcctatcag
    54721 caaggtactg ctctcaattt cttccttctt tccatcaact tctacgtgaa acgagtagga
    54781 ttgtgtggaa gctaactgtt tgattcctga catgaaataa gctagttttc caaatttcgt
    54841 cttcttctcc gcatctacat cattgattga ttcagggata gagccaattg cgacgacatt
    54901 cataaaatag tcgtcattta tttttccaat atccaatggc ttgactgatt caatcgaaaa
    54961 atgattgatc gcttcctcag gttctagcgg tatccctaat gctcgagcca agtcattcac
    55021 cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct tgttcagcga ttccactgat
    55081 tccttcgttg actgttccat ctccgcccat gacaaacaca ctatgatacc cttccgtagc
    55141 cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt gtatgcaaga cgaccacttc
    55201 atcaaacacc gattccaatt ttgctatcgc taattgctca aattcctttg cttgttcacc
    55261 gcctgaactg gggtttacca ctaataaaac ttttttcatc actttctttc ccttctgatt
    55321 gatttcttta atataacgat agcataaaga aaaaaattag gctgtgatga agtactgttc
    55381 atgaaacatt catttatttt ttgacaagat aaaacatcca tactatgaaa ctgaggaaaa
    55441 ttaaaacaat gatataggaa ataaaagaaa atgataggca ttgtatacct aataaagtaa
    55501 aaatcttagg tggcagataa aaacacaaaa atagcaagag aatgtaatag cgtttcttcc
    55561 actttttgtt atcagacctt atagcgatca gaggaatacc aaatgcataa ctgaaaaact
    55621 cttgatgtac tgttaatatg tcacggcctt gaataataaa tgcgacaatc cccgctataa
    55681 ttagtgtgcc taacaccaaa acggaaaatt gatgaatata ttttatatac aaatgatcca
    55741 ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc gtatacgtga ataacttcta
    55801 gaaataaaag aaatttgcaa aaccactcca
//
LOCUS       DAYYCM010000012        55148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000012 DAYYCM010000000
VERSION     DAYYCM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.012"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHAY1_000806"
     CDS             3..371
                     /locus_tag="ACHAY1_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8592821.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHAY1_000807"
     CDS             551..1492
                     /locus_tag="ACHAY1_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8592822.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHAY1_000808"
     CDS             1561..2292
                     /locus_tag="ACHAY1_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8592823.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHAY1_000809"
     CDS             2436..4616
                     /locus_tag="ACHAY1_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8592824.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHAY1_000810"
     CDS             4636..5118
                     /locus_tag="ACHAY1_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8592825.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHAY1_000811"
     CDS             5134..5895
                     /locus_tag="ACHAY1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8592826.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHAY1_000812"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHAY1_000812"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8592827.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHAY1_000813"
     CDS             7667..8152
                     /locus_tag="ACHAY1_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8592828.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHAY1_000814"
     CDS             8145..8828
                     /locus_tag="ACHAY1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8592829.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHAY1_000815"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHAY1_000815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8592830.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHAY1_000816"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHAY1_000816"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8592831.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHAY1_000817"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHAY1_000817"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8592832.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHAY1_000818"
     CDS             13125..13457
                     /locus_tag="ACHAY1_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8592833.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHAY1_000819"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHAY1_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8592834.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHAY1_000820"
     CDS             14283..15656
                     /locus_tag="ACHAY1_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8592835.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHAY1_000821"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHAY1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8592836.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHAY1_000822"
     CDS             16711..17151
                     /locus_tag="ACHAY1_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8592837.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHAY1_000823"
     CDS             17295..17996
                     /locus_tag="ACHAY1_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8592838.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHAY1_000824"
     CDS             17993..19114
                     /locus_tag="ACHAY1_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8592839.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHAY1_000825"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHAY1_000825"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8592840.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHAY1_000826"
     CDS             20493..21401
                     /locus_tag="ACHAY1_000826"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8592841.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHAY1_000827"
     CDS             21436..21708
                     /locus_tag="ACHAY1_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8592842.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHAY1_000828"
     CDS             21719..22765
                     /locus_tag="ACHAY1_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8592843.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACHAY1_000829"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACHAY1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8592844.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACHAY1_000830"
     CDS             complement(25776..26462)
                     /locus_tag="ACHAY1_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592845.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACHAY1_000831"
     CDS             complement(26440..26934)
                     /locus_tag="ACHAY1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8592846.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACHAY1_000832"
     CDS             complement(26954..28174)
                     /locus_tag="ACHAY1_000832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592847.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACHAY1_000833"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACHAY1_000833"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8592848.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACHAY1_000834"
     CDS             30352..31263
                     /locus_tag="ACHAY1_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592849.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32274..32510
                     /locus_tag="ACHAY1_000835"
     CDS             32274..32510
                     /locus_tag="ACHAY1_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592850.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32623..32865
                     /locus_tag="ACHAY1_000836"
                     /pseudo
     CDS             32623..32865
                     /locus_tag="ACHAY1_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32955..33194
                     /locus_tag="ACHAY1_000837"
     CDS             32955..33194
                     /locus_tag="ACHAY1_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592851.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="ACHAY1_000838"
     CDS             33341..37624
                     /locus_tag="ACHAY1_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8592852.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="ACHAY1_000839"
     CDS             37723..38088
                     /locus_tag="ACHAY1_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592853.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="ACHAY1_000840"
     CDS             38090..38542
                     /locus_tag="ACHAY1_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592854.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            38991..40466
                     /locus_tag="ACHAY1_000841"
     CDS             38991..40466
                     /locus_tag="ACHAY1_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8592855.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="ACHAY1_000842"
     CDS             40489..40800
                     /locus_tag="ACHAY1_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592856.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="ACHAY1_000843"
     CDS             41160..42416
                     /locus_tag="ACHAY1_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8592857.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="ACHAY1_000844"
     CDS             42413..42958
                     /locus_tag="ACHAY1_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592858.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="ACHAY1_000845"
     CDS             42959..43459
                     /locus_tag="ACHAY1_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592859.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43818..44096
                     /locus_tag="ACHAY1_000846"
     CDS             43818..44096
                     /locus_tag="ACHAY1_000846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592860.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            44116..44460
                     /locus_tag="ACHAY1_000847"
     CDS             44116..44460
                     /locus_tag="ACHAY1_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592861.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="ACHAY1_000848"
     CDS             44479..45390
                     /locus_tag="ACHAY1_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8592862.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="ACHAY1_000849"
     CDS             45469..46461
                     /locus_tag="ACHAY1_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8592863.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="ACHAY1_000850"
     CDS             46458..46676
                     /locus_tag="ACHAY1_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8592864.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="ACHAY1_000851"
     CDS             46689..47099
                     /locus_tag="ACHAY1_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8592865.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="ACHAY1_000852"
     CDS             47151..49679
                     /locus_tag="ACHAY1_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8592866.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="ACHAY1_000853"
     CDS             49695..51905
                     /locus_tag="ACHAY1_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8592867.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="ACHAY1_000854"
     CDS             51913..53004
                     /locus_tag="ACHAY1_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8592868.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="ACHAY1_000855"
     CDS             53025..53567
                     /locus_tag="ACHAY1_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8592869.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="ACHAY1_000856"
     CDS             53762..54019
                     /locus_tag="ACHAY1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592870.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="ACHAY1_000857"
     CDS             54033..54365
                     /locus_tag="ACHAY1_000857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592871.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54410..54958
                     /locus_tag="ACHAY1_000858"
     CDS             54410..54958
                     /locus_tag="ACHAY1_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8592872.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54965..>55148
                     /locus_tag="ACHAY1_000859"
     CDS             54965..>55148
                     /locus_tag="ACHAY1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592873.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLP"
BASE COUNT        18736 a         8982 c        11878 g        15552 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgtttcag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtaggt caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac
    54481 tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa
    54541 attagtagat gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt
    54601 ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg
    54661 aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg
    54721 gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc
    54781 tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc
    54841 ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc
    54901 taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa
    54961 gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa
    55021 tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat
    55081 gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt
    55141 tttaccag
//
LOCUS       DAYYCM010000013        55093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000013 DAYYCM010000000
VERSION     DAYYCM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.013"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACHAY1_000860"
     CDS             complement(26..328)
                     /locus_tag="ACHAY1_000860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8592874.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACHAY1_000861"
     CDS             complement(353..2446)
                     /locus_tag="ACHAY1_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8592875.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACHAY1_000862"
     CDS             complement(2935..3699)
                     /locus_tag="ACHAY1_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8592876.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACHAY1_000863"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACHAY1_000863"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8592877.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACHAY1_000864"
     CDS             complement(4704..5780)
                     /locus_tag="ACHAY1_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8592878.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACHAY1_000865"
     CDS             complement(5804..6397)
                     /locus_tag="ACHAY1_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8592879.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACHAY1_000866"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACHAY1_000866"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8592880.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACHAY1_000867"
     CDS             complement(8067..8366)
                     /locus_tag="ACHAY1_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8592881.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACHAY1_000868"
                     /pseudo
     CDS             complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACHAY1_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10126..11037)
                     /locus_tag="ACHAY1_000869"
     CDS             complement(10126..11037)
                     /locus_tag="ACHAY1_000869"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8592882.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11157..12014
                     /locus_tag="ACHAY1_000870"
     CDS             11157..12014
                     /locus_tag="ACHAY1_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8592883.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12201..12563)
                     /locus_tag="ACHAY1_000871"
     CDS             complement(12201..12563)
                     /locus_tag="ACHAY1_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8592884.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12752..12934)
                     /locus_tag="ACHAY1_000872"
     CDS             complement(12752..12934)
                     /locus_tag="ACHAY1_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592885.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12909..14348
                     /locus_tag="ACHAY1_000873"
     CDS             12909..14348
                     /locus_tag="ACHAY1_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592886.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14355..16907)
                     /locus_tag="ACHAY1_000874"
     CDS             complement(14355..16907)
                     /locus_tag="ACHAY1_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8592887.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17021..18352)
                     /locus_tag="ACHAY1_000875"
     CDS             complement(17021..18352)
                     /locus_tag="ACHAY1_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8592888.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18318..19796)
                     /locus_tag="ACHAY1_000876"
     CDS             complement(18318..19796)
                     /locus_tag="ACHAY1_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8592889.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="ACHAY1_000877"
     CDS             complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="ACHAY1_000877"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8592890.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22406..23833
                     /locus_tag="ACHAY1_000878"
     CDS             22406..23833
                     /locus_tag="ACHAY1_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592891.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23925..24290)
                     /locus_tag="ACHAY1_000879"
     CDS             complement(23925..24290)
                     /locus_tag="ACHAY1_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8592892.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24287..24652)
                     /locus_tag="ACHAY1_000880"
     CDS             complement(24287..24652)
                     /locus_tag="ACHAY1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8592893.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24844..25092)
                     /locus_tag="ACHAY1_000881"
     CDS             complement(24844..25092)
                     /locus_tag="ACHAY1_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592894.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25091..25219
                     /locus_tag="ACHAY1_000882"
     CDS             25091..25219
                     /locus_tag="ACHAY1_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8592895.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25276..26970
                     /locus_tag="ACHAY1_000883"
     CDS             25276..26970
                     /locus_tag="ACHAY1_000883"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8592896.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27070..27243
                     /locus_tag="ACHAY1_000884"
     CDS             27070..27243
                     /locus_tag="ACHAY1_000884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592897.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27621..28754
                     /locus_tag="ACHAY1_000885"
     CDS             27621..28754
                     /locus_tag="ACHAY1_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8592898.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28785..29105
                     /locus_tag="ACHAY1_000886"
     CDS             28785..29105
                     /locus_tag="ACHAY1_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8592899.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29134..29598)
                     /locus_tag="ACHAY1_000887"
     CDS             complement(29134..29598)
                     /locus_tag="ACHAY1_000887"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592900.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29730..30983)
                     /locus_tag="ACHAY1_000888"
     CDS             complement(29730..30983)
                     /locus_tag="ACHAY1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8592901.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31012..31329)
                     /locus_tag="ACHAY1_000889"
     CDS             complement(31012..31329)
                     /locus_tag="ACHAY1_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8592902.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31355..31675)
                     /locus_tag="ACHAY1_000890"
     CDS             complement(31355..31675)
                     /locus_tag="ACHAY1_000890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8592903.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32139..33554)
                     /locus_tag="ACHAY1_000891"
     CDS             complement(32139..33554)
                     /locus_tag="ACHAY1_000891"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8592904.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33571..34887)
                     /locus_tag="ACHAY1_000892"
     CDS             complement(33571..34887)
                     /locus_tag="ACHAY1_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8592905.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35037..35735)
                     /locus_tag="ACHAY1_000893"
     CDS             complement(35037..35735)
                     /locus_tag="ACHAY1_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8592906.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36024..37013)
                     /locus_tag="ACHAY1_000894"
     CDS             complement(36024..37013)
                     /locus_tag="ACHAY1_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8592907.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37093..37926)
                     /gene="msrA"
                     /locus_tag="ACHAY1_000895"
     CDS             complement(37093..37926)
                     /gene="msrA"
                     /locus_tag="ACHAY1_000895"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8592908.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38101..39735)
                     /locus_tag="ACHAY1_000896"
     CDS             complement(38101..39735)
                     /locus_tag="ACHAY1_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8592909.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39722..41410)
                     /locus_tag="ACHAY1_000897"
     CDS             complement(39722..41410)
                     /locus_tag="ACHAY1_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592910.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            41556..43193
                     /locus_tag="ACHAY1_000898"
     CDS             41556..43193
                     /locus_tag="ACHAY1_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592911.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(43338..44078)
                     /gene="truA"
                     /locus_tag="ACHAY1_000899"
     CDS             complement(43338..44078)
                     /gene="truA"
                     /locus_tag="ACHAY1_000899"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8592912.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(44269..44904)
                     /locus_tag="ACHAY1_000900"
     CDS             complement(44269..44904)
                     /locus_tag="ACHAY1_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8592913.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(45051..46271)
                     /gene="sstT"
                     /locus_tag="ACHAY1_000901"
     CDS             complement(45051..46271)
                     /gene="sstT"
                     /locus_tag="ACHAY1_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8592914.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            46490..47488
                     /locus_tag="ACHAY1_000902"
     CDS             46490..47488
                     /locus_tag="ACHAY1_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592915.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            47684..49519
                     /locus_tag="ACHAY1_000903"
     CDS             47684..49519
                     /locus_tag="ACHAY1_000903"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8592916.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(49579..50295)
                     /locus_tag="ACHAY1_000904"
     CDS             complement(49579..50295)
                     /locus_tag="ACHAY1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8592917.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(50522..51064)
                     /locus_tag="ACHAY1_000905"
     CDS             complement(50522..51064)
                     /locus_tag="ACHAY1_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8592918.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(51069..51296)
                     /locus_tag="ACHAY1_000906"
     CDS             complement(51069..51296)
                     /locus_tag="ACHAY1_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8592919.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(51375..53255)
                     /locus_tag="ACHAY1_000907"
     CDS             complement(51375..53255)
                     /locus_tag="ACHAY1_000907"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8592920.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(53444..54058)
                     /locus_tag="ACHAY1_000908"
     CDS             complement(53444..54058)
                     /locus_tag="ACHAY1_000908"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8592921.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            54200..54760
                     /locus_tag="ACHAY1_000909"
     CDS             54200..54760
                     /locus_tag="ACHAY1_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8592922.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT        16886 a        11133 c         9130 g        17944 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tgcattggct aagcctgctt gtttttgaac
     8821 agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
     8881 catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg
     8941 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc
     9001 aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa
     9061 ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt
     9121 actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt
     9181 ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc
     9241 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
     9301 tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc
     9361 gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat
     9421 tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
     9481 aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
     9541 agaatcgaca acttcgttaa cttgaacgat cacgatacca tctttgaaag ctgcagcttc
     9601 gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc
     9661 tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag
     9721 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
     9781 acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa
     9841 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
     9901 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
     9961 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    10021 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    10081 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    10141 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    10201 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    10261 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    10321 agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    10381 cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca tatacggctt
    10441 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    10501 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    10561 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    10621 aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac
    10681 gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag
    10741 ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat
    10801 cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa
    10861 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    10921 atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    10981 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    11041 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    11101 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    11161 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    11221 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    11281 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    11341 aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat
    11401 tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg
    11461 tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga
    11521 tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga
    11581 tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta
    11641 agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca
    11701 tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga
    11761 aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg
    11821 tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg
    11881 atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt
    11941 ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt
    12001 atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga
    12061 acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga
    12121 gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc
    12181 ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct
    12241 agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg
    12301 tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc
    12361 ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct
    12421 ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa
    12481 tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg
    12541 attagttctt ttttcgttag catctgcttc catctctttc ttattgattc gctttattca
    12601 aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa tacttcgata
    12661 tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct
    12721 actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt
    12781 tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg
    12841 aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag
    12901 tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg
    12961 tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa
    13021 tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc
    13081 ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt
    13141 tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga
    13201 aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt
    13261 cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact
    13321 tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta
    13381 ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt
    13441 cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg
    13501 ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag
    13561 cgaattgccg attagattct tctatccata ccaacagcac actctcctcc ctgtatgact
    13621 ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt
    13681 ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg
    13741 ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag
    13801 cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc
    13861 tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc
    13921 gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac
    13981 tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt
    14041 ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
    14101 ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag
    14161 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa
    14221 aaacagatat cttgatttcc aatttccctc tagattctct agatatccct attgtataca
    14281 tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
    14341 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
    14401 ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
    14461 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
    14521 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
    14581 ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
    14641 acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca
    14701 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
    14761 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
    14821 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
    14881 acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc
    14941 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
    15001 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
    15061 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct
    15121 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt
    15181 tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca
    15241 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
    15301 atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
    15361 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt
    15421 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
    15481 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
    15541 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
    15601 tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc
    15661 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
    15721 atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca
    15781 acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc
    15841 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
    15901 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
    15961 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
    16021 ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
    16081 aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
    16141 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
    16201 atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta
    16261 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
    16321 agcgttcctg aaaaacccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
    16381 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
    16441 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
    16501 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
    16561 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
    16621 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
    16681 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca
    16741 gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
    16801 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
    16861 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
    16921 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
    16981 gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata tttctattgc
    17041 ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa
    17101 agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag
    17161 caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg
    17221 cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct
    17281 gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca
    17341 ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat
    17401 ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg tagactgcac
    17461 tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt
    17521 ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc
    17581 atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag
    17641 gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta
    17701 agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc
    17761 cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag
    17821 actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc
    17881 ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa
    17941 ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta
    18001 caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc
    18061 agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa
    18121 cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca
    18181 tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg
    18241 atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga
    18301 ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca
    18361 cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt
    18421 tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg
    18481 atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca
    18541 ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt
    18601 cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag
    18661 acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca
    18721 ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg
    18781 gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct
    18841 tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact
    18901 gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga
    18961 atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt
    19021 acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg
    19081 gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat atacacagag
    19141 gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca
    19201 gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg
    19261 atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac
    19321 aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa
    19381 aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca
    19441 agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc gaccatcacc
    19501 atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct
    19561 agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc agcaaattca
    19621 ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc
    19681 atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag
    19741 gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt
    19801 attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga
    19861 aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg
    19921 atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata
    19981 aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg
    20041 aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt
    20101 ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt ttgtataaaa
    20161 attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat
    20221 tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg
    20281 agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata
    20341 aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc
    20401 gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg
    20461 attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa
    20521 ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac
    20581 attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga
    20641 tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt caaacacata
    20701 tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc
    20761 agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat
    20821 agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg
    20881 ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa
    20941 cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc
    21001 atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc
    21061 tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag ctaattcacg
    21121 aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac
    21181 catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt
    21241 ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc
    21301 tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac
    21361 agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt
    21421 gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa gattagcaat
    21481 agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc
    21541 ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt
    21601 tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt
    21661 catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg
    21721 actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag
    21781 cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag
    21841 atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc
    21901 gcctataaag acggccttga tatccatatc tttcattttt cttccctcca tattttgttg
    21961 gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa
    22021 tgagaatttt ttttttataa catcaaatcc acgtagattt ttacaaacaa aaataaataa
    22081 agtcggattt ttctgataat tcaacgcaaa aaagcgaaca atttttcaat aaatcagtct
    22141 tctaaaatag ataacctaaa aaaagcgaac aaacaaataa aatgactgtc cttcattcac
    22201 taaacatatt tttatttttt tatattgtta aaaattcgca atttattttt taatagaatt
    22261 atctgttttc caaattttat aacataaaaa acctttttta acggcattta tatgcttttt
    22321 cagaaattaa ctgatgattt caaaaagaaa acatgtcata atatttccca tattgtgcaa
    22381 aaaaatacaa ggcggaggct ctactatgat tgaagaatat attgagaaaa atattttaag
    22441 acagctgttt ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc
    22501 caatctcctg catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga
    22561 atttgaagaa cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc
    22621 agaacatata cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa
    22681 aacttgtctg ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga
    22741 attcatctct gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga
    22801 ctgcgggatc gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct
    22861 gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt
    22921 ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga
    22981 taaagaaaat aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt
    23041 gtgtgctgtg acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa
    23101 ttatgtcgaa tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt
    23161 cgcctttatt ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat
    23221 tgcggaagac tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga
    23281 actagttgct acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga
    23341 acgtgcgctg attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga
    23401 aaaattttat cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt
    23461 ttccagctgg tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc
    23521 ttttgtgatc gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat
    23581 cgtcacaaat tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac
    23641 tttcgatttt caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc
    23701 gcagcagtcc tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca
    23761 atatgaaaat attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt
    23821 gcaaaacaaa taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg
    23881 cccatttctg taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca
    23941 tctacgggtc cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac
    24001 gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct
    24061 gaaacaagaa tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg
    24121 ttctcgctga ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc
    24181 ttgattgata gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt
    24241 acaagttttt cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc
    24301 ctcttctttg tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc
    24361 gaatgataat aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact
    24421 cgatgatatc agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt
    24481 ttcttgtatc tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat
    24541 caaaaagttt gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat
    24601 ttaaaatagc aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta
    24661 cctactttct tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc
    24721 tatttaaaag ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat
    24781 ttttctccct ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa
    24841 ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa
    24901 atttctaaaa atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt
    24961 atttacagga aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc
    25021 tatcatttgg cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga
    25081 ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct
    25141 tggcgctgca tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga
    25201 tgaattcgaa tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac
    25261 attggaggaa tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat
    25321 atggcagcgg ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt
    25381 tattcattgg atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa
    25441 tattggaata aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc
    25501 atgcttaatt accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt
    25561 actgagccag ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc
    25621 tttgacaaag cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga
    25681 ttcaataata aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa
    25741 gaaaaactag acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa
    25801 acaaacttct ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa
    25861 gaattgcgtc gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg
    25921 ggcgtcaatc gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac
    25981 ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa
    26041 gaaacaccga tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact
    26101 caagaagaag aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc
    26161 attacagatt cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat
    26221 ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct
    26281 gacaaattct ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa
    26341 aaccatttgt tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg
    26401 aattcattta tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct
    26461 attgtcgtgc atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg
    26521 ggttatttcc tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg
    26581 aatggaaaag aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca
    26641 gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc
    26701 tatatgcctt atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc
    26761 attcctaaac attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag
    26821 atgatcttta ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg
    26881 ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta
    26941 gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat
    27001 caagtaataa gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta
    27061 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agctgccctg
    27121 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
    27181 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
    27241 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
    27301 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
    27361 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
    27421 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
    27481 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
    27541 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
    27601 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
    27661 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
    27721 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
    27781 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
    27841 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
    27901 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
    27961 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
    28021 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
    28081 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
    28141 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
    28201 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
    28261 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
    28321 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
    28381 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
    28441 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
    28501 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
    28561 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
    28621 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
    28681 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
    28741 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
    28801 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
    28861 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
    28921 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
    28981 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
    29041 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
    29101 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
    29161 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
    29221 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
    29281 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
    29341 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
    29401 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
    29461 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
    29521 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
    29581 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
    29641 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa
    29701 caatcacttg ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct
    29761 tggatcgtag ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca
    29821 gcggctagta caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga
    29881 gttacccatg gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa
    29941 gcaacactaa ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac
    30001 acgataggta acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac
    30061 aagtttgtta caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt
    30121 aaacctaaag ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc
    30181 acgataaacg gtgcgctatg tccagctgcg attgcattaa cgtttgcttc aatggctggc
    30241 gcattgattg tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat
    30301 aagaatggtg tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata
    30361 aatggttctt ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca
    30421 aaaattgcag caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca
    30481 aatgcttttg caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt
    30541 ccgcttaaac gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa
    30601 ccgtttgagc tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca
    30661 cctgcggaaa caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca
    30721 atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat
    30781 gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg
    30841 atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga
    30901 atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc
    30961 ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt
    31021 caatcgacga tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat
    31081 cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc
    31141 ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt
    31201 gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc
    31261 atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg
    31321 atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt
    31381 ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag
    31441 gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc
    31501 ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata
    31561 cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta
    31621 ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa
    31681 acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc
    31741 gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa
    31801 aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca
    31861 caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag
    31921 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    31981 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    32041 tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata
    32101 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    32161 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    32221 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    32281 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    32341 tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct
    32401 tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct
    32461 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    32521 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    32581 acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg
    32641 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    32701 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    32761 aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    32821 tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca
    32881 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    32941 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    33001 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    33061 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    33121 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    33181 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    33241 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    33301 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    33361 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    33421 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    33481 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    33541 atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc
    33601 tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat
    33661 aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca
    33721 agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt
    33781 tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg
    33841 cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt
    33901 gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt
    33961 gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg
    34021 tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg
    34081 ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc
    34141 aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg
    34201 tacgacattg taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc
    34261 cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga
    34321 aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca
    34381 gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc
    34441 tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc
    34501 ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc
    34561 atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa
    34621 gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg
    34681 caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt
    34741 gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat
    34801 gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc
    34861 cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt
    34921 gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca
    34981 aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa
    35041 ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc catatggaca
    35101 gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta
    35161 tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca
    35221 attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca
    35281 atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa
    35341 gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc
    35401 gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc
    35461 atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta
    35521 tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc
    35581 cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca
    35641 ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca
    35701 gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat
    35761 gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa
    35821 ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg
    35881 ataaaattca ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc
    35941 aataacagac gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga
    36001 taaaaagttt gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct
    36061 ggtgagaaat ctaaattatc catcattcga acactttctt gtaactgact taaccgactt
    36121 gctccgacca gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc
    36181 gctaatgatt gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt
    36241 gatccaactt gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca
    36301 ggaataccat gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt
    36361 gcccctagcc cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa
    36421 aacatattgt agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca
    36481 gtcatttttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    36541 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    36601 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    36661 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    36721 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    36781 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    36841 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    36901 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    36961 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    37021 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    37081 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    37141 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    37201 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    37261 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    37321 cgctaattct ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct
    37381 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    37441 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    37501 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    37561 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    37621 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    37681 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    37741 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    37801 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    37861 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    37921 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    37981 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    38041 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    38101 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    38161 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    38221 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    38281 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    38341 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    38401 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    38461 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    38521 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    38581 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    38641 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    38701 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    38761 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    38821 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    38881 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    38941 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    39001 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    39061 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    39121 aataaaaata aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta
    39181 cactagcaaa ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga
    39241 gatgatttga taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc
    39301 aattgcggaa cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact
    39361 aaataaaaat gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag
    39421 tgttgtacac aagttcaata accaaacgta aataatatag cttacaatca aattttttgt
    39481 catccaatat aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc
    39541 atatccagta taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc
    39601 cttgattcga gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt
    39661 gaaaaagatg aaagaatata tactactaac tataattaat ccactcaata ttactacttt
    39721 tttatttatt ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca
    39781 acttattagg tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac
    39841 tgatccataa agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt
    39901 ctccagcatt tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt
    39961 taccgtttac ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct
    40021 ttttgttgct tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt
    40081 cccacgattc tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt
    40141 ctgttggggc gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt
    40201 cttcattgat aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg
    40261 ctgctataga cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt
    40321 ttttattcat agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt
    40381 ggatacctaa gatacgccaa ggaaactgga gtaagcttat cggtgttttt tgaaacaaaa
    40441 accatggaaa taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt
    40501 tttctttttt ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta
    40561 ataaccccat agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa
    40621 tagaaagtgc ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca
    40681 tctgctgcaa cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct
    40741 ttactaaatt cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac
    40801 gaactataat catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat
    40861 acacaagtag acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca
    40921 atccataagc ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg
    40981 cggatcgata atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata
    41041 aaaaagcagc atctatcttt ttaaaaaact tttctccata ataaaaacta atcagaatcg
    41101 tacaaatatt taataaccaa atatatagaa cataacttaa tattaagtta tttgaaattc
    41161 cataaaaaac gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc
    41221 cataattgtt aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga
    41281 ttcgtgaaag atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa
    41341 aatttactga atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac
    41401 ttattttcat agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt
    41461 ctttttttaa taagttttgc gttaaatttt tcatagcact agtatgatat actagaaaag
    41521 aatcaatttt tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa
    41581 tagatatcat ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta
    41641 agggaatcta tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac
    41701 tagataatgt atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga
    41761 tgaatatttt ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg
    41821 gaaatcttgt tttaagttat aatatctact atttttttat cactttttta acaatggtcg
    41881 tttcttattt ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga
    41941 ttatttatac attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag
    42001 aagctgtcgc tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta
    42061 gagattacca aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt
    42121 tactttctgt cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt
    42181 tttgggacca aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt
    42241 ttttaagtgc gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag
    42301 tcccactggg aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa
    42361 ataataacta taacaactat actattggat tattcttatt tctcggattg attggtgctt
    42421 ttatttttat aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg
    42481 ttcttttttg ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga
    42541 gtctacaatt tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga
    42601 gtatagtgat ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta
    42661 cgtttcttgc actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag
    42721 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
    42781 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
    42841 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
    42901 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
    42961 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
    43021 ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac
    43081 tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
    43141 ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta
    43201 tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc
    43261 tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat
    43321 ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg
    43381 acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc
    43441 ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat
    43501 aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat
    43561 tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa
    43621 tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga
    43681 gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa
    43741 tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg
    43801 attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc
    43861 agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca
    43921 tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt
    43981 tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt
    44041 tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt
    44101 ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct
    44161 ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca
    44221 gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag
    44281 atgcaagata ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca
    44341 aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata
    44401 aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat
    44461 ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta
    44521 acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt
    44581 attgatactg aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc
    44641 tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa
    44701 tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg
    44761 actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata
    44821 caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa
    44881 accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat
    44941 tgttattata aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat
    45001 ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca
    45061 cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct
    45121 agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa
    45181 tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag
    45241 ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa
    45301 gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct
    45361 tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg
    45421 cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc
    45481 aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa
    45541 gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata
    45601 aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa
    45661 acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga
    45721 taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac
    45781 aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct
    45841 agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga
    45901 acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt
    45961 gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata
    46021 actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg
    46081 aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga
    46141 ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc
    46201 catgtgggga aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga
    46261 actaacgaca tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat
    46321 cattgtatca ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac
    46381 aaacaaccat ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat
    46441 tctaagttag gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc
    46501 caattttttt gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct
    46561 gacaatcttt gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac
    46621 tattcctaca tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc
    46681 cgttgttatt ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc
    46741 ccgtttaaaa gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact
    46801 agtttgcaat atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct
    46861 tatcctaaca cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt
    46921 ttgtagtatt ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata
    46981 tctactatta tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc
    47041 gtttgcatta cagaatggac agagtatttt atggattttt tatttatttt taatgggtta
    47101 tggtttgaaa gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat
    47161 cagcttgatt gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag
    47221 aggaaataca gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta
    47281 tactttctct tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag
    47341 aggaaatttt cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt
    47401 ttcttatcgc gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct
    47461 gataaatatc gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt
    47521 tttattttat atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga
    47581 ttcgattgaa caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa
    47641 aagttatttt ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt
    47701 agaaccaaaa gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg
    47761 acaagcaccc atgatcctga caatcatcat tattatggga tttttcttct tactattgtt
    47821 gatttccaat cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac
    47881 tatttcgaca gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa
    47941 aatgctgacc ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg
    48001 cggattgatc cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa
    48061 acagtattcc ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg
    48121 atgtttttcg ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa
    48181 tttatttaaa gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc
    48241 agtcattcaa ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc
    48301 gaaagaaaaa attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca
    48361 aacaagaagt gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag
    48421 tgatcctgca cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag
    48481 cattataaca gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc
    48541 taacatggaa tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt
    48601 tacaccttat acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt
    48661 cgatgaaaaa atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt
    48721 tgataaattt ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac
    48781 ggataaaata gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa
    48841 agctttacaa agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca
    48901 tatgccttat gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat
    48961 tcctaataga agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc
    49021 agtaaaaaaa ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta
    49081 tggtgaccat ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca
    49141 gcaccagaca gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt
    49201 aaataaagaa gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat
    49261 caaagtaact ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac
    49321 aattgatcct acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga
    49381 ggatagtcaa attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga
    49441 ttatcgtctg atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac
    49501 acaaaaaata aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac
    49561 aaccccttgt ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt
    49621 caattcatta ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg
    49681 ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt
    49741 cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc
    49801 tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc
    49861 cccgttcaaa atagcttgag ggactttaag accatataag tccacactca tttgattcgt
    49921 ataacttttg tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg
    49981 tgcggatttg tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga
    50041 agatgaagcc tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga
    50101 agactctgac gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc
    50161 tgtgctagtt gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt
    50221 tttcgattca ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa
    50281 aatccttttt ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa
    50341 taagcaatgt gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa
    50401 aactgagaag cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta
    50461 atcttcgtga ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact
    50521 tttagtcatc atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt
    50581 gtacttggta gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc
    50641 catatttttc ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat
    50701 caaaatcttt tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat
    50761 gttcgtaaat ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt
    50821 caatcactaa ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg
    50881 aaaaaagggt acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca
    50941 atttcatttc ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg
    51001 gtttatgatg gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat
    51061 tcattgattt acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca
    51121 actgcagcta attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc
    51181 gcattaaaca aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt
    51241 ttttgcatac agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta
    51301 gttcttcctt cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg
    51361 attcttatca agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc
    51421 atcgcgttaa agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg
    51481 taaataaacc cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact
    51541 acattttcac gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat
    51601 tcaaaattag attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat
    51661 cccatagcaa tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca
    51721 ataaaagcaa cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct
    51781 ggtaataatt cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc
    51841 atctgattat cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct
    51901 aattgttcag cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt
    51961 ttgtcgattg ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt
    52021 acttgctttg gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa
    52081 actgtatgac catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg
    52141 actgctaatt gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc
    52201 agaggatgat cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc
    52261 tctgtatagt tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta
    52321 tcaaagacaa aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag
    52381 atgccccgtt tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact
    52441 aaagcgccag gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc
    52501 agaccaacaa ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca
    52561 atgaattttt ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg
    52621 gcaaaggtcg tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa
    52681 atcgttccag agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc
    52741 actgcggctt catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct
    52801 ccaccagttt ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca
    52861 gtcgttccat cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg
    52921 attgactccc gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag
    52981 aatgtaacga tagctgactc cttgtattct ccgatcataa aggcaccaag cacagcaata
    53041 gtaacgagta aatcaattga gataactttg actttcactg cctgatacgc atggatcata
    53101 ataggtatga ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa
    53161 ccagtaaatt ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga
    53221 tttcgatgct cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc
    53281 ttacgagact aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt
    53341 caaaaaggcg gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca
    53401 ctaaaagtga tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc
    53461 tgtacagcat acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt
    53521 cctttagtct ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa
    53581 aaaggaatat ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga
    53641 tgccttttgt tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat
    53701 gcttctttaa atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt
    53761 ctttagcaaa tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag
    53821 aacgatgttt ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct
    53881 ctgcatacaa agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc
    53941 cgtaaagatt cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag
    54001 caaaatagat agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca
    54061 tttcgcctcc tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta
    54121 ctatctataa tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt
    54181 cggataagga gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc
    54241 tttggctgaa acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg
    54301 cgaactaaca gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat
    54361 ctatgatcta tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc
    54421 ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc
    54481 aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa
    54541 agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac
    54601 gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt
    54661 tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt
    54721 acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta
    54781 gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta
    54841 ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg
    54901 ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta
    54961 ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata
    55021 gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt
    55081 tcaaggggaa tca
//
LOCUS       DAYYCM010000014        50133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000014 DAYYCM010000000
VERSION     DAYYCM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.014"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHAY1_000910"
     CDS             33..656
                     /locus_tag="ACHAY1_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592923.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHAY1_000911"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHAY1_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8592924.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHAY1_000912"
     CDS             2152..3033
                     /locus_tag="ACHAY1_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592925.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHAY1_000913"
     CDS             3036..3806
                     /locus_tag="ACHAY1_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592926.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHAY1_000914"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHAY1_000914"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8592927.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHAY1_000915"
     CDS             5117..6280
                     /locus_tag="ACHAY1_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8592928.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHAY1_000916"
     CDS             6397..6807
                     /locus_tag="ACHAY1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8592929.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHAY1_000917"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHAY1_000917"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8592930.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHAY1_000918"
     CDS             9380..9664
                     /locus_tag="ACHAY1_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592931.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHAY1_000919"
     CDS             9824..10060
                     /locus_tag="ACHAY1_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8592932.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHAY1_000920"
     CDS             10057..10455
                     /locus_tag="ACHAY1_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8592933.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="ACHAY1_000921"
     CDS             complement(11122..12066)
                     /locus_tag="ACHAY1_000921"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8592934.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="ACHAY1_000922"
     CDS             complement(12362..13306)
                     /locus_tag="ACHAY1_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8592935.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="ACHAY1_000923"
     CDS             13527..14741
                     /locus_tag="ACHAY1_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8592936.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="ACHAY1_000924"
     CDS             14763..16778
                     /locus_tag="ACHAY1_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8592937.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="ACHAY1_000925"
     CDS             complement(16861..18234)
                     /locus_tag="ACHAY1_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8592938.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="ACHAY1_000926"
     CDS             complement(18260..18544)
                     /locus_tag="ACHAY1_000926"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8592939.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="ACHAY1_000927"
     CDS             complement(18546..20561)
                     /locus_tag="ACHAY1_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8592940.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="ACHAY1_000928"
     CDS             complement(20740..21045)
                     /locus_tag="ACHAY1_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8592941.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="ACHAY1_000929"
     CDS             complement(21134..22165)
                     /locus_tag="ACHAY1_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8592942.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="ACHAY1_000930"
     CDS             22372..23247
                     /locus_tag="ACHAY1_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8592943.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="ACHAY1_000931"
     CDS             complement(23399..24241)
                     /locus_tag="ACHAY1_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8592944.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="ACHAY1_000932"
     CDS             complement(24333..26210)
                     /locus_tag="ACHAY1_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8592945.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="ACHAY1_000933"
     CDS             complement(26207..27628)
                     /locus_tag="ACHAY1_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8592946.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="ACHAY1_000934"
     CDS             complement(27631..31020)
                     /locus_tag="ACHAY1_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8592947.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32668
                     /locus_tag="ACHAY1_000935"
     CDS             31277..32668
                     /locus_tag="ACHAY1_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592948.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32741..33388)
                     /locus_tag="ACHAY1_000936"
     CDS             complement(32741..33388)
                     /locus_tag="ACHAY1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8592949.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33396..34082)
                     /locus_tag="ACHAY1_000937"
     CDS             complement(33396..34082)
                     /locus_tag="ACHAY1_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592950.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34088..35161)
                     /locus_tag="ACHAY1_000938"
     CDS             complement(34088..35161)
                     /locus_tag="ACHAY1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592951.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35383..36057
                     /locus_tag="ACHAY1_000939"
     CDS             35383..36057
                     /locus_tag="ACHAY1_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8592952.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36062..37402
                     /locus_tag="ACHAY1_000940"
     CDS             36062..37402
                     /locus_tag="ACHAY1_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8592953.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37551..38537)
                     /locus_tag="ACHAY1_000941"
     CDS             complement(37551..38537)
                     /locus_tag="ACHAY1_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8592954.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39261..40292
                     /locus_tag="ACHAY1_000942"
     CDS             39261..40292
                     /locus_tag="ACHAY1_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8592955.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40401..41933
                     /locus_tag="ACHAY1_000943"
     CDS             40401..41933
                     /locus_tag="ACHAY1_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8592956.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42072..42884
                     /locus_tag="ACHAY1_000944"
     CDS             42072..42884
                     /locus_tag="ACHAY1_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8592957.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42874..43359
                     /locus_tag="ACHAY1_000945"
     CDS             42874..43359
                     /locus_tag="ACHAY1_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8592958.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43382..43927
                     /locus_tag="ACHAY1_000946"
     CDS             43382..43927
                     /locus_tag="ACHAY1_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8592959.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(44000..45643)
                     /locus_tag="ACHAY1_000947"
     CDS             complement(44000..45643)
                     /locus_tag="ACHAY1_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8592960.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45772..48000)
                     /locus_tag="ACHAY1_000948"
     CDS             complement(45772..48000)
                     /locus_tag="ACHAY1_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8592961.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48033..48512)
                     /locus_tag="ACHAY1_000949"
     CDS             complement(48033..48512)
                     /locus_tag="ACHAY1_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8592962.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            complement(48777..49736)
                     /locus_tag="ACHAY1_000950"
     CDS             complement(48777..49736)
                     /locus_tag="ACHAY1_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8592963.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            49905..>50133
                     /locus_tag="ACHAY1_000951"
     CDS             49905..>50133
                     /locus_tag="ACHAY1_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8592964.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT        15260 a         9231 c         9411 g        16231 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttttaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 aggaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    32101 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    32161 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    32221 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    32281 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    32341 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    32401 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    32461 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    32521 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    32581 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    32641 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    32701 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    32761 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    32821 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    32881 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    32941 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    33001 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    33061 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    33121 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    33181 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    33241 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    33301 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    33361 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    33421 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    33481 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    33541 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    33601 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    33661 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    33721 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    33781 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    33841 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    33901 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    33961 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    34021 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    34081 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    34141 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    34201 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    34261 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    34321 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    34381 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    34441 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    34501 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    34561 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    34621 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    34681 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    34741 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    34801 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    34861 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    34921 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    34981 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    35041 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    35101 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    35161 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    35221 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    35281 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    35341 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    35401 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    35461 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    35521 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    35581 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    35641 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    35701 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    35761 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    35821 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    35881 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    35941 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    36001 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    36061 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    36121 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    36181 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    36241 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    36301 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    36361 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    36421 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    36481 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    36541 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    36601 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    36661 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    36721 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    36781 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    36841 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    36901 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    36961 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    37021 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    37081 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    37141 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    37201 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    37261 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    37321 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    37381 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    37441 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    37501 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    37561 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    37621 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    37681 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    37741 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    37801 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    37861 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    37921 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    37981 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    38041 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    38101 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    38161 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    38221 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    38281 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    38341 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    38401 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    38461 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    38521 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    38581 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    38641 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    38701 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg
    38761 tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat
    38821 tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac
    38881 tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa
    38941 taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa
    39001 gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag
    39061 ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta
    39121 aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta
    39181 agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac
    39241 actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa
    39301 agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt
    39361 ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt
    39421 tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag
    39481 attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga
    39541 accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt
    39601 cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc
    39661 ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac
    39721 ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag
    39781 ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt
    39841 gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc
    39901 agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag
    39961 cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt
    40021 ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga
    40081 gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt
    40141 atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc
    40201 tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat
    40261 tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat
    40321 aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa
    40381 caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta
    40441 cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac
    40501 tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt
    40561 tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt
    40621 ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag
    40681 tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt
    40741 tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa
    40801 atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg
    40861 tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa
    40921 ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc
    40981 aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag
    41041 tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc
    41101 ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg
    41161 cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag
    41221 aagctttttc gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa
    41281 tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc
    41341 aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg
    41401 atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag
    41461 ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg
    41521 ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg
    41581 atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag
    41641 tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt
    41701 tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta
    41761 ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac
    41821 ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa
    41881 tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta
    41941 aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat
    42001 aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg
    42061 aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag
    42121 gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta agcgccttga
    42181 cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat
    42241 tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg
    42301 ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa
    42361 atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc
    42421 aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta
    42481 atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga
    42541 tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca
    42601 caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat
    42661 tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg
    42721 caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa
    42781 aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct
    42841 ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat
    42901 gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct
    42961 acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt
    43021 ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga
    43081 tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt
    43141 tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt
    43201 atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc tggagtggct
    43261 tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact
    43321 gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa
    43381 aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt
    43441 ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt
    43501 cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac
    43561 gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt
    43621 gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc
    43681 tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa
    43741 tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc
    43801 aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg
    43861 acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa
    43921 ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat
    43981 gaactttaac gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta
    44041 gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact
    44101 gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa
    44161 gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat
    44221 attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct
    44281 tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg
    44341 tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag
    44401 tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt
    44461 cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat
    44521 caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa
    44581 taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc
    44641 aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca
    44701 tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt
    44761 tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa
    44821 attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag
    44881 tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat
    44941 cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg
    45001 cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta
    45061 tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt
    45121 cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat
    45181 gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat
    45241 ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt
    45301 cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga
    45361 tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct
    45421 cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga
    45481 tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga
    45541 tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt
    45601 gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta
    45661 acggtccctc ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag
    45721 aaacgaaaag ctcaacgaat ctgctaagtg cgttcgttga gctttatttt atcatagata
    45781 gcgggcaatc agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa
    45841 acgactttca ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac
    45901 aaataggatt ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt
    45961 tttgcctgta taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt
    46021 ggttgttgcg gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg
    46081 attgttttgc cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc
    46141 aaattcttta taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc
    46201 gtaaatcaac aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt
    46261 ttttgccgct tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc
    46321 taccgctaaa gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc
    46381 atatgctact ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc
    46441 tgtttgttca ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat
    46501 tacttgcatg tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac
    46561 gatgagacct tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag
    46621 ttgcttcttg atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc
    46681 atctcctgtt tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg
    46741 catttgagga ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt
    46801 tggttcttca gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc
    46861 agtgatgttt cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga
    46921 ttgaggatga ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact
    46981 gtcagacaaa gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa
    47041 aacttttgga taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata
    47101 aaaataagaa cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa
    47161 caaacggata atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc
    47221 catcgcacgt tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat
    47281 ccgatctctt aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat
    47341 cacaatgacg caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt
    47401 tggttcgagc gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc
    47461 ttgaatctct aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct
    47521 gtaatggatt gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc
    47581 tttagcagaa aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag
    47641 tgtatctgtt tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac
    47701 tacaggtaat aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt
    47761 tgtataggga agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc
    47821 ggcatctcct tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt
    47881 gatttcggag gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca
    47941 gatcatttgc ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat
    48001 gcttctcttc cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt
    48061 tttaaaatac ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac
    48121 aaaatgatgt cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc
    48181 ccttgtaatc caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga
    48241 taagcatctt ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt
    48301 gtgattggta attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg
    48361 cctgagatat cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt
    48421 gcatccatat ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact
    48481 ggtttatgtt ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt
    48541 agtaaataag taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga
    48601 ttgaagtgag gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt
    48661 tcttttacac gctcattagt aacatgtaga aagtccaaga tgatgttgga atattttggc
    48721 tctatacttt ttgatgttgg tgaacttcac caacatcttt tttgtattct taattgtcaa
    48781 attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga
    48841 taatccagtg attttctagg aatattattt cgtttagatg cgacagattg aataaaaaat
    48901 tcatctactg aattgaaatc catagatttc aataaaccat ctctacgtaa caatccgtta
    48961 gaattctcgt ttaggcctct ttgagacggc gttcctggat cagcgaaata aatggcaata
    49021 tcattgacat tactgatctg tttccaattt gaaaattcct ttccacaatc aaaagtgatg
    49081 gatttgaata ggtttttcgg tacagattca aaccattgat ttaatttttt ttcaatatca
    49141 atcgcttgtc taccacacgg tttcaatgtg atgataactt ttgataatcg ttcaactaag
    49201 gtaattacag cacttttatg tttcagacct acgatagtgt caccttcaag gtgaccaaac
    49261 tcatttgaga attggctata atccttttca cgttcatgaa tagagcggcg gaaagtttgt
    49321 tttccacgtc tttcttgatg tccattcggt ttacgcttgc ctttcatcgg taagtcagaa
    49381 gaatcaaata gccccttttt gaacatacga taaatggtac gagcagaaca actaatagga
    49441 aacgctgcac gaccaacaat cacatcgggt gtccatcctt gaacaacctt tctttgaata
    49501 tattctgatt gttcctcggg taaaacaagc ggacgtctac cacagtttga tttgtttttc
    49561 ttatactgtt gataatattc taaggctgat tttccttgct tgaagaacag atatacttta
    49621 tggatcgttt gtcttgaacg attcaagcaa tgggcagttt tagcaacaga ttgattcatt
    49681 ttgaaataag actctattat tacaagttca tccgttgtaa gatgggtata ggtcatttgt
    49741 actcactcct tataattctt tggtcggaac tattttgagt gtagcacaaa tgactttttt
    49801 agttgtctag cttaatttta caatcggcgt ataaaaaaat atagaaaaga caccctgaaa
    49861 tcctttagaa ttaatttggc taccaattca taggaggatt caagatgtct catactcagc
    49921 ttatcaaaga taccctaaat attctagacc taaatatcta tttcgaggaa aattgtttaa
    49981 ctaaagaaaa atacaaaggg caaatttgct tgatttacaa aggaactctt ctgtacaagc
    50041 cagaagagtg ttcccattgt ctttgtgtcg tcccctctag gatcattcgt tgggggacga
    50101 caaccgttcg gttgttactg aatgatgtgt ctg
//
LOCUS       DAYYCM010000015        45276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000015 DAYYCM010000000
VERSION     DAYYCM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.015"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..573
                     /locus_tag="ACHAY1_000952"
     CDS             <1..573
                     /locus_tag="ACHAY1_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8592965.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            585..1505
                     /locus_tag="ACHAY1_000953"
     CDS             585..1505
                     /locus_tag="ACHAY1_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592966.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1546..3003
                     /locus_tag="ACHAY1_000954"
     CDS             1546..3003
                     /locus_tag="ACHAY1_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8592967.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3348..3962
                     /locus_tag="ACHAY1_000955"
     CDS             3348..3962
                     /locus_tag="ACHAY1_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8592968.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3955..5679
                     /locus_tag="ACHAY1_000956"
     CDS             3955..5679
                     /locus_tag="ACHAY1_000956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8592969.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5676..7127
                     /locus_tag="ACHAY1_000957"
     CDS             5676..7127
                     /locus_tag="ACHAY1_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8592970.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7600..9753)
                     /locus_tag="ACHAY1_000958"
     CDS             complement(7600..9753)
                     /locus_tag="ACHAY1_000958"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8592971.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9755..10801)
                     /locus_tag="ACHAY1_000959"
     CDS             complement(9755..10801)
                     /locus_tag="ACHAY1_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8592972.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10951..12240
                     /locus_tag="ACHAY1_000960"
     CDS             10951..12240
                     /locus_tag="ACHAY1_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8592973.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12253..14955
                     /locus_tag="ACHAY1_000961"
     CDS             12253..14955
                     /locus_tag="ACHAY1_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8592974.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            14940..15797
                     /locus_tag="ACHAY1_000962"
     CDS             14940..15797
                     /locus_tag="ACHAY1_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8592975.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15868..16725)
                     /locus_tag="ACHAY1_000963"
     CDS             complement(15868..16725)
                     /locus_tag="ACHAY1_000963"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8592976.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            16936..19107
                     /locus_tag="ACHAY1_000964"
     CDS             16936..19107
                     /locus_tag="ACHAY1_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8592977.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19175..19516
                     /locus_tag="ACHAY1_000965"
     CDS             19175..19516
                     /locus_tag="ACHAY1_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8592978.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19792..21546)
                     /locus_tag="ACHAY1_000966"
     CDS             complement(19792..21546)
                     /locus_tag="ACHAY1_000966"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8592979.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21557..22393)
                     /locus_tag="ACHAY1_000967"
     CDS             complement(21557..22393)
                     /locus_tag="ACHAY1_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8592980.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22395..23078)
                     /locus_tag="ACHAY1_000968"
     CDS             complement(22395..23078)
                     /locus_tag="ACHAY1_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8592981.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23237..24631)
                     /gene="uxaC"
                     /locus_tag="ACHAY1_000969"
     CDS             complement(23237..24631)
                     /gene="uxaC"
                     /locus_tag="ACHAY1_000969"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8592982.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24670..26169)
                     /locus_tag="ACHAY1_000970"
     CDS             complement(24670..26169)
                     /locus_tag="ACHAY1_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8592983.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26162..27583)
                     /locus_tag="ACHAY1_000971"
     CDS             complement(26162..27583)
                     /locus_tag="ACHAY1_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8592984.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27643..28635)
                     /locus_tag="ACHAY1_000972"
     CDS             complement(27643..28635)
                     /locus_tag="ACHAY1_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8592985.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            28867..31065
                     /locus_tag="ACHAY1_000973"
     CDS             28867..31065
                     /locus_tag="ACHAY1_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8592986.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31523..32395)
                     /locus_tag="ACHAY1_000974"
     CDS             complement(31523..32395)
                     /locus_tag="ACHAY1_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8592987.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            32695..33669
                     /locus_tag="ACHAY1_000975"
     CDS             32695..33669
                     /locus_tag="ACHAY1_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8592988.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            33691..34383
                     /locus_tag="ACHAY1_000976"
     CDS             33691..34383
                     /locus_tag="ACHAY1_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8592989.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            34349..35857
                     /locus_tag="ACHAY1_000977"
     CDS             34349..35857
                     /locus_tag="ACHAY1_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8592990.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            35931..37034
                     /locus_tag="ACHAY1_000978"
     CDS             35931..37034
                     /locus_tag="ACHAY1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8592991.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            37037..38374
                     /locus_tag="ACHAY1_000979"
     CDS             37037..38374
                     /locus_tag="ACHAY1_000979"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8592992.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            38361..39239
                     /locus_tag="ACHAY1_000980"
     CDS             38361..39239
                     /locus_tag="ACHAY1_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8592993.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            39236..40159
                     /locus_tag="ACHAY1_000981"
     CDS             39236..40159
                     /locus_tag="ACHAY1_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8592994.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            40156..40566
                     /locus_tag="ACHAY1_000982"
     CDS             40156..40566
                     /locus_tag="ACHAY1_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8592995.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            40570..42138
                     /locus_tag="ACHAY1_000983"
     CDS             40570..42138
                     /locus_tag="ACHAY1_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8592996.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            42378..43796
                     /locus_tag="ACHAY1_000984"
     CDS             42378..43796
                     /locus_tag="ACHAY1_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8592997.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            43808..45118
                     /locus_tag="ACHAY1_000985"
     CDS             43808..45118
                     /locus_tag="ACHAY1_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8592998.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        14678 a         7976 c         9270 g        13352 t
ORIGIN      
        1 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
       61 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
      121 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
      181 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
      241 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
      301 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
      361 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
      421 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
      481 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
      541 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
      601 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
      661 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
      721 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
      781 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
      841 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
      901 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttaatta
      961 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
     1021 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
     1081 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
     1141 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
     1201 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
     1261 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
     1321 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
     1381 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
     1441 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
     1501 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
     1561 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
     1621 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
     1681 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
     1741 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
     1801 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
     1861 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
     1921 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
     1981 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
     2041 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
     2101 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
     2161 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
     2221 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
     2281 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
     2341 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
     2401 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
     2461 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
     2521 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
     2581 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
     2641 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
     2701 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
     2761 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
     2821 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
     2881 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
     2941 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
     3001 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
     3061 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
     3121 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
     3181 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
     3241 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
     3301 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
     3361 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
     3421 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
     3481 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
     3541 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
     3601 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
     3661 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
     3721 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
     3781 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
     3841 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
     3901 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
     3961 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
     4021 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
     4081 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
     4141 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
     4201 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
     4261 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
     4321 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
     4381 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
     4441 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
     4501 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
     4561 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
     4621 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
     4681 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
     4741 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
     4801 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
     4861 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
     4921 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
     4981 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
     5041 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
     5101 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
     5161 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
     5221 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
     5281 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
     5341 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
     5401 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
     5461 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
     5521 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
     5581 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
     5641 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
     5701 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
     5761 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
     5821 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
     5881 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
     5941 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
     6001 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
     6061 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
     6121 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
     6181 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
     6241 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
     6301 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
     6361 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
     6421 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
     6481 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
     6541 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
     6601 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
     6661 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
     6721 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
     6781 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
     6841 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
     6901 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
     6961 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
     7021 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
     7081 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa aaaaagccaa
     7141 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
     7201 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
     7261 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
     7321 actcgaaact aaacagcttt ttcacgacct ctccactaaa tggtgttcaa tcagctgacc
     7381 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
     7441 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
     7501 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
     7561 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
     7621 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
     7681 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
     7741 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
     7801 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
     7861 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
     7921 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
     7981 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
     8041 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
     8101 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
     8161 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
     8221 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
     8281 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
     8341 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
     8401 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
     8461 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
     8521 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
     8581 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
     8641 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
     8701 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
     8761 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
     8821 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
     8881 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
     8941 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
     9001 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
     9061 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
     9121 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
     9181 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
     9241 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
     9301 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
     9361 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
     9421 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
     9481 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
     9541 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
     9601 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
     9661 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
     9721 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
     9781 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
     9841 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
     9901 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
     9961 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    10021 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    10081 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    10141 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    10201 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    10261 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    10321 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    10381 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    10441 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    10501 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    10561 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    10621 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    10681 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    10741 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    10801 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    10861 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    10921 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    10981 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    11041 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    11101 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    11161 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    11221 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    11281 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    11341 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    11401 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    11461 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    11521 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    11581 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    11641 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    11701 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    11761 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    11821 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    11881 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    11941 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    12001 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    12061 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    12121 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    12181 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    12241 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    12301 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    12361 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    12421 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    12481 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    12541 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    12601 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    12661 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    12721 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    12781 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    12841 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    12901 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    12961 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    13021 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    13081 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    13141 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    13201 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    13261 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    13321 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    13381 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    13441 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    13501 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    13561 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    13621 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    13681 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    13741 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    13801 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    13861 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    13921 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    13981 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    14041 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    14101 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    14161 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    14221 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    14281 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    14341 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    14401 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    14461 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    14521 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    14581 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    14641 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    14701 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    14761 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    14821 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    14881 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    14941 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    15001 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    15061 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    15121 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    15181 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    15241 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    15301 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    15361 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    15421 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    15481 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    15541 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    15601 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    15661 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    15721 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    15781 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    15841 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    15901 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    15961 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    16021 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    16081 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    16141 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    16201 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    16261 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    16321 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    16381 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    16441 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    16501 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    16561 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    16621 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    16681 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    16741 agtgcaatcg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    16801 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    16861 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    16921 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    16981 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    17041 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    17101 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    17161 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    17221 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    17281 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    17341 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    17401 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    17461 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    17521 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    17581 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    17641 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    17701 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    17761 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    17821 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    17881 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    17941 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    18001 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    18061 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    18121 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    18181 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    18241 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    18301 cctactgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    18361 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    18421 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    18481 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    18541 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    18601 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    18661 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    18721 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    18781 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    18841 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    18901 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    18961 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    19021 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    19081 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    19141 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    19201 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    19261 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    19321 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    19381 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    19441 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    19501 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    19561 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    19621 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    19681 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    19741 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    19801 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    19861 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    19921 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    19981 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    20041 tatcaacgct cgtgcaatgg agatacgttg cttttggcca ccagacagct tgttgccatg
    20101 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    20161 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    20221 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    20281 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    20341 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    20401 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    20461 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    20521 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    20581 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    20641 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    20701 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    20761 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    20821 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    20881 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    20941 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    21001 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    21061 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    21121 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    21181 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    21241 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    21301 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    21361 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    21421 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    21481 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    21541 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    21601 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    21661 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    21721 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    21781 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    21841 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    21901 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    21961 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    22021 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    22081 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    22141 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    22201 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    22261 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    22321 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    22381 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    22441 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    22501 ttgtctttta gtccttctgg gtaattttta caagtatcag gtataagatg caggtagaat
    22561 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    22621 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    22681 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    22741 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    22801 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    22861 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    22921 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    22981 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    23041 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    23101 tttataggca atgaagcaga acagcaagag aagaagacgg agcaaatttg gtccgtcttc
    23161 ttctctaaaa cgtggataaa cggattccgt tatttcacta tcctgttttt gattcgttac
    23221 tgttactatt aatcaattag gcaaaaaagc caaagtaatt tttagcattt gtataactaa
    23281 tcgcttgaac tagacttcct aaaagttgtt catcttcagg tgcttctcct cgcatgacca
    23341 tttcgcctaa aaattcacaa agaacacgac ggaaatattc atgtctagtg taggacagga
    23401 aacttcttga atctgtcagc atgccgacaa aattccccaa agcactacca tcagcaaatt
    23461 gtgtcaattg atcgcgcata ccagctcgag tatcgttgta ccaccatcca gaacctaact
    23521 gcagttttcc attctgttct ttttggaaac agcccatcaa agctaaaatc gcaggatagt
    23581 cattcggatt caatgaataa aggatcgtct ttggcaactg atccgtttct tctgcacggt
    23641 tcaataattt ttgcagagga atagtcaaag aaagatcatt cattccatca tagccggtat
    23701 cgggccctaa tttttccaac atttgtgtat tacagttacg ataagcatgg atgtgcagct
    23761 gcatcgtcca attatgagaa tgatagaagt gggtcaaatg tatcagtgtt tctgtacgat
    23821 aagcatcgat ttcatctgaa gataatagtt tgttagctcg tgctttttga aaaatctctt
    23881 ctaatactgt ttcatccgct tctttatagc gtaagatgtc taaagcgtgg tcggaaacac
    23941 gacatccatg ttgatggaag tattctatcc gctgccctaa gacttctatc agttcttgat
    24001 aggtggtgat cgtttggtca gctgcttctt ccagtagtgt gacccatgca ttaaatcctt
    24061 ctttttcaat attgagtgct ttatctggac ggaatgaagg tagaaccttc aaatctggtt
    24121 cttcatgagc aagcaactga tgatagtgca agtcgtctgc gggatcatct gttgtacaaa
    24181 tcacttctac attcgatttt tttgcgattt cccgacggcg aaaatcagga gctgccaaaa
    24241 tttcatttgc ttgtgtccaa attttctctg cattttcttc atttatcaac aagtcaatac
    24301 cgaagaaacg cttcaattca agatgtgacc aactgtaaag cggatttccg ataatttttg
    24361 gtacggtttg acaccaagct aaaaattttt caaaatcaga agcatttcct gtgatttttt
    24421 cttcaggcac gccgcaagct cgcatcaagc gccatttgta atgatcgcct cctaaccaag
    24481 cttctgtcag attgctgaaa ttgttgtttt cgtaaatttc ttttggatct aagtgacagt
    24541 gataatcaat aatgggcatg ttttttgcgt aggaatggta aagctttttc gcccaatcgt
    24601 cttttaataa aaaatcatca gataaaaaca taaatcttcc ttctttcttt ttatactgtt
    24661 ttatgaaaat caaagtgtta ctccgttttt aaaaatggca atttctcgaa cgtcattttg
    24721 ttcatttttc gtttcttctc cactagctac agcgatgatt ttttgaacaa acgattctaa
    24781 gattacttcc attggttgtt ctaataagac gcctgcatta aaatccatcc aatgaccttt
    24841 gcgttcaaaa agagttgtat tcgtagatac cttgaccgtt gggacatatg agccaaacgg
    24901 agtccctctt cctgtggtaa acaagaccaa ctggcaatcg gatgaagcaa gagcagaggc
    24961 agctacaaga tcattcccgg gagcttgcaa taaactaagc cctggttttc tgattttttc
    25021 gccatattgt agcacatcaa cgacaggaga gcttcctgat ttttgcgtgc agcctaaaga
    25081 cttatcttct aatgtagtga tcccacctgc tttattacca ggagatgggt tttcgtaaac
    25141 tggttccccg taagacaaga aatactgttt gaagtcattg atcaagtcaa cgattgagtc
    25201 aaaaacttct tcattttccg ctcgtgccat caatacttgt tctgcgccaa acatttctgg
    25261 cacttctgtt aagatcgtgc tgcctccttg agcaatcaag tagtcagaaa atgcaccaag
    25321 taaaggatta gcagttattc ccgatagacc atcagaaccg ccgcatttca aaccgacatt
    25381 caacttagag agcgggactg atatgcgctg gtcattttcc gccgctgtta aaagctcttc
    25441 taatagagcc acaccttgtt cgatctcatc gtaaacatct tgagccacta gaaatttcac
    25501 tcgttcttca tcatattcac ctaaaatttt tttgaattca ggaactgtat tgttctcaca
    25561 acctaatcca aatactaaaa ctcctccggc attgggatga tttactgcgt ccgctaaaat
    25621 ttctcgagta ttttgatgat cttttccaag ctgcgaacag ccataaggat gctttaaaat
    25681 cgtaatatgg tcaaactgtc ccagatctgg atgtttttcc ttgaactgtt tgacgatcag
    25741 ttcagcaatg ccattcacac acccaacggt aggtactaaa aacaaatcat tacgaatccc
    25801 tacttttcca ttttttcgca agtaaccttg aaatgttcgg ttctcaaaaa ggtaatgact
    25861 tggatggaca tctttttcat atcgataatc caattcacca gaaagattgg tttttacatt
    25921 atgagtgtgc agccattgac cggcagttac gtctgtcgtc acatgaccga ttggatagcc
    25981 atatttgatc acgttgtctc cagctttcaa atcacccaat gcaatcttat gaccttgcgg
    26041 gatatcttca agagtggtga tcgtttgctg atcaatcgtc agttgtgttt gttttggaat
    26101 aggggttaat gcgacgacca cactgtctcg tcgatttaat ttcattgttg gttgcagggt
    26161 ttcaagcatg gaccatcgtt cctttcattt cttgtaatac tgaccggaca cctttttgat
    26221 cgatctttgc agctatttct tgaactgtat ctgtcacatt cggtatttga gacaaatctt
    26281 ctccccaaag cgcagcgttt tgcaataatc gaggaatagc tgttccatta tcagaccaag
    26341 catcagtaaa ttcgcttatc gtcttttcat catcatgtgg ctttacttga tcgcctcgat
    26401 acataagaag tagcgcagct agtgaaacag tgattaacgg tggtacctga ttttgttctt
    26461 cgatatactt cttgaacact ggaagcagac gggtgctgaa ctttgaaaca ctatttaatg
    26521 aaatcgcttc taatttgtga tgggcaaaag gattcaagaa acgttctttg atttggtcag
    26581 cataaatggc taattcatct ttaggtaaag aaagcatagg aatcaattct agattgaata
    26641 actgatcaac atatgcggag aataaaggat ctttcattac ttcttctaca gtttccaatt
    26701 ccgccaacaa agcaagaggg accatcgctg tatgcggtcc attaagcaaa tgcaccttac
    26761 gttcacgata aggagtcata tcatctgtga tgatgacatt caagccagcc tgtttgaatg
    26821 gtagttgatt ctccaattct ttaggccctt cgatcaccca aagcatgaat ggttctgctt
    26881 tgaccatcaa ttgatcttca tagttatgcc gttgattgaa aacacctgca tcttcgcgag
    26941 gataacctgg gacgatgcga tcaactaaac tacaacagaa aacattttct ttttctaacc
    27001 actcaacaaa agcaggttct aaatcccatt tttctgcgta gcgtaaaatg atttctttta
    27061 gacgttcgcc gtttctgtca atcagttcgc aaggaatgat ggtaaagcca gctttgttta
    27121 atttgaaacg tcggtataaa aaggctgtca gtttgcccgg gaagctctgt gggggagtat
    27181 ctgttaaatt atcagtctcc acgaattgga ttcctgcctc tgttgtattg gagacgatat
    27241 aggccaagtc gtcattttct gctagagata gataagcatc ccattcatca taaggattga
    27301 tcatttgttc tactacggtg ataatctcgg aagtatcaac gatttcgcca ttgctgagtc
    27361 cttgtaaaat gaccgtatat aaattatctt gctggttcat catatttcca agaccttgtt
    27421 ctaaaggttg aatgattgca acattacctt taaacaattg ttggttattc agctgctgaa
    27481 tctgccaatc aataaatcca cgcataaaat tgccttcacc aaactgaaga acttttacag
    27541 gtgcttcgtt ttttttcact aattcttttg tcagtttttt catataaact tcctttctgt
    27601 gttatgcaca cgttaacatt tttatataaa attacttgta gtttacaagt aattgatcga
    27661 ctcacgaagg atcaacaagg tggagagctc aattttagat tgttcgatcg tcctgccttc
    27721 gatcaaatct agtaaaagct ttgttccttc ttgacacatt tctttcaaag gcttacgtac
    27781 ggtggtaagc ggcggattga gataggagac gactggagaa tcatcgaacc cgatcagaga
    27841 aatgttctgg ggaacacgga ggccagctaa atgtacagct ctcaatgcac caatcgctat
    27901 gtcgtcgttg ccgcaaaaaa ccagagtagg caggtcagaa agtgataata ggtattccat
    27961 gttttggagg ccgctttcta agctgtaatc tcctgttaaa aaataggctg gattgacagg
    28021 gatgctcgct ttttttagac ttttcaatac ccctttttcc cgttcttttg tcgaacgaaa
    28081 atttttcttt ccgccaatat aagcgatctt cgtatgtccg catccaatac cgtaatctac
    28141 tgcctgcata acaccggccg catcatcggc agtgacgttg ataaccgctg gatcattgat
    28201 gtgacgattg acaacaacga aaggaaattc tttttctttg agatagtcga tgaaaggctg
    28261 atcatcatca ctttgactca taacgatcag tccatcgtag cgttgcaggt tgatttctgt
    28321 taaatcaaca acatcgatac tttcaacaga taaactgtaa tttgtgtcta atactgaatg
    28381 gatgcctgca atggaatctg ctaggaaatt tgatgaagtt cctttatgca aacttgagaa
    28441 aaatagtccg attagatatt ttttttgatt cactaaacct tttgcactat agtttggcga
    28501 ataattcatt tccttagcta gttgtcgtat tttttgtttt gtttcatttt ttattaaact
    28561 gctgttattc aaagcacgag aaactgtcgt atgagaaacg ccagctgctt tagcgatatc
    28621 tttgatcgtt accattccat cattttcctt actttttcac tttttgttaa cgcattcatc
    28681 atattacgat aactacgata agtcaataga tctttgatat gaaaagattg cacacgttaa
    28741 aaattacttg gatttttatt tttaattatg ctattcttga aagcggggaa tgctcattta
    28801 attagatgag tatcaatttt acagaaaaat gaaagccttt tattttaaaa ggaggagagg
    28861 aagaaaatga agaaaagtac agtattttat aaaatatttt taccgatgtt tttattagga
    28921 gccgtacttg tcataggatt tagtttattc atttaccaga atacctatga atcgatcgaa
    28981 tcgagttatt taacagacaa aaagaatttg ctcaggcaag ttaaaactaa cgttgaatgg
    29041 aaaattagaa cgattgaata ttcgttttct acgtatggtt cgaccaaaaa tttctcggac
    29101 atattcaagc atccgctgac atatacagat tactccactt attctgaaat caggaaagaa
    29161 ctaaatttta tagaaacgat tgttatggat gataataatt atgatttagt cagtttggct
    29221 ggtaaatggg gagtaatcaa tggatcgttg agtcaactga ccgaagagga agttgaaaag
    29281 tatcaacaga agtacataaa taacaaaaat aatttatttt ggcaaaagaa aaacaaagga
    29341 atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa aatatgcatt gggaattgct
    29401 acgatagaga aacatacaat agaacaagtt gttggtgaaa gtggagaaga catactttct
    29461 attgaaaacg aagttggcaa cctttttact actcagacag taaaaaataa agagcaacca
    29521 actgtattaa cggacgtgga tctagattac aatcagctga aagtgataag agaaaaagat
    29581 aaaacgtata tagtactgaa atcagattat aataattgga tttacagatt agaagttgac
    29641 tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg ggctagttgc agtgtcttta
    29701 acactggtag gactcattgg tcttctttcc tacttatttt cggacagatt tgcccgtccg
    29761 atttctcaaa tacaggaacg tctgaatctt aaatcaaaag gattcagtgg gaaagaacta
    29821 gatttggtcg ctcaatcagt cagtcggatc ataggagaaa aagaagcgtt aagtgcacac
    29881 ttagttaccc aaaagccaca gctggaaacg ctatttgtac ttagtttatt tagaaatcga
    29941 gtagaaaggc gagaactaga tcagcggttg cagcaatttg gttatagttc tcaaaatgac
    30001 tgctattata cagctttggt tcaaatcgat ctgttagaag atagtcacgg tggggagcgt
    30061 gatttgctac ttttagcgat caacaacatg atcacggaaa ttgtgccaaa ggaaaatcga
    30121 atgctgccga tcgttttgaa tgaagaaatg caagccacta tttatcgaat ggataaagat
    30181 gatgcagaag caagcaaaaa agtcatggag tattgccgac tcattcaaaa acgtgtaaaa
    30241 gaatacttga atttaactat cagtataggc atcagtaacc ggttcgaaac gctgaatgaa
    30301 agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt atcgtgtaaa tactggtcca
    30361 agttcaatta ttttttatca ggaaatcgtt cctgctgctc atgaaaaaac acttattaga
    30421 tatccgatag aagaacaaaa tcgattgttt gaagccattc gttctggaaa tcaggaagtg
    30481 aagaatttag tacatgaatt gattgatgca ttgtttgcac aaaataaaaa tcctttgagt
    30541 cgggaagtag tttcggtccg cttgatcaat gaactcgttc aattaggaca gttattggga
    30601 gtagattcaa aaaactttga taacatgaaa caaatttatg taaaagcact gaacaattat
    30661 catcccaaag aactagaaaa acttgtttta gaacaattag ttctgccaat cacagaatgc
    30721 tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg agaatattat gcatatcgtc
    30781 tacacagaat atgatcgtga tctgtcttta gatgtgatcg ctgataggtt gcattataac
    30841 cctaactatt taagtaatgt atttaaaaag gaaacaggag aaaaatttgg tgattttgtt
    30901 caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag agactaacct ctccgtaaaa
    30961 gagattgcag aaagattgca atatcgaaat ccgcaaaatt ttattcgttt ctttaaaaag
    31021 aaggaatcga ttacgccggg agaatatcga aaaaaatatt tgtgaatcaa gaggctgttt
    31081 ttcttgtgga ctgattcaag caataaaaga agttagaata accctagtat ttgtatttat
    31141 ggatagttaa atttcaaggc taagcacgca aaaagaactg tgacaaaagt cttgtcacag
    31201 ttctttttta cgaataaatg gcgttcaaga gacaggctat cgacaataag gcataatttt
    31261 gtgaaatatg ggaagctatt tttcaaaatc gcttcttatt gctcctgtgg gttactcgtc
    31321 gcaatgtttg tgagagtttt tatcatcgct caaggctgaa cgtctctttc acaacctctg
    31381 atacacggtg ttccaggagt agattcttcg aaaaataagc cgaaatttca caaaaattta
    31441 agaagcaatt ttcgtaaatg ccttcttatt tcttgaagct gaccacttct gacacaacta
    31501 ccattttgcg ttcagtacat aatcaccctt tgacagaacc tacgagtgct cctttgacaa
    31561 aatatttctg aacaaaagga taaacaatca gcataggcac tgttgcaata acgataacag
    31621 ccattttaac tgtttgtgct ggtggaatca catcaacggc actactttct gcttgcatcc
    31681 cacttgatac aatgacgatt tggcgtaaaa gaatctgtat cggccattta gatgaatcgt
    31741 tgatatacaa aatagcagtc atataagtgt tccagtaagt aacagcataa aaaagcgaga
    31801 tagtagcaat agacggtaac gctaatggca gcataatttt gaaaaataca ccaaaatctg
    31861 tgcaaccatc catttttgct gattcttcca aactatccgg caaggcttgg aaaaagtttc
    31921 tcataatgat catattcatt gcattaacag ccacaggcag gatcatggcc cagtaagaat
    31981 tgaccagccc tagagaacga acaactaaga acgttggaat catccccccg ctgaacaaca
    32041 tagaaaatac aacaagaaag ttgacgaaac ctcgaccaaa caaatacttt ctggaaagac
    32101 cgtaggccat cagtgaagtt gcacacatac tgacgatcgt tccgacacct gtaacaccaa
    32161 tcgaaacagc aagtgctcta aaaatggtcg gcgtagaaag aatgtagcga tacgcgtcta
    32221 aagaaaaggt agtaggaaaa aggataaact ttttcgcgac gacttcttgt gtagaagcaa
    32281 aagatgtagc gatgatattt aaaaaaggaa cgatacagat caaagctagc aaaattagaa
    32341 acgtattgtt gaaaagtgta aaacttcttt ctcctataga gactttcttt ttcatataga
    32401 taggctcctc ctcaaaataa attaaatgtt tctgtttcta tatgttcaat ttatcatcgg
    32461 tgaaagatta cgtatataat catttcctat gctaagtgtc agatagaaaa aaatagaagg
    32521 tttgctatgc gtagtttatt tgtgaaaaaa tacactttat cgttttgtat agcacaaaaa
    32581 tcattgctat gtaagcgttt ttacttacgg taaaaatgat tatatacgta attcagatag
    32641 ttatctatca tgaagtcata agcagcaaac attcacaaaa ctaaggaggg gcttatggaa
    32701 agtgtaaaga aacaagaacc atttcaactg acgctatcga aaaaagaaga aaagctcaga
    32761 agacgaaaaa aaataattgc aggaatcaaa acgaataaat ttttatatgt gatgattctg
    32821 ccgggagtcc tttatttctt gatttttagg tatcttccta tggggggact cgttattgct
    32881 tttcaagatt atcaaccatt tttaggaata atgggaagtc cttgggtcgg tttcaaacat
    32941 ttcattcgtt tgtttacaga accgacattc tttacattat taagaaatac gttgatcttg
    33001 ttcggattaa atatcttcgt gttttttccg atgccaatca ttttgtcttt actattaaat
    33061 gaagtaacga acaaacgatt gaaaaacggg attcaaacga tcatttacat ccctcacttc
    33121 atgtcttggg taatcattgt ttcgattact tatgtatttt tgaatgttga cggaggggta
    33181 ttgaatgaat tccttgcaac aatgggattt gaaaaaatca gttttctaac atcccctgaa
    33241 tggacacgca cagtatatat cggacaagtg atctggaaag agttgggctg gtctacgatc
    33301 atctaccttt cagcgataac tgcagtcgat acacagttgt atgaagctgc agaaatggat
    33361 ggagcaggac gcttacgcaa aacatggcat gttacattgc cggcaattcg tccagtaatc
    33421 attaccttgc tgattctaaa aatcggtcag acattagact tagggttcga gcatatgtac
    33481 ttgttattga attcattgaa tcgaagcgtt gcagaaattt tcgacaccta tatttataca
    33541 gctggattga agaatggaca gttaagtttc agtacagcaa tgggattgtt taaaggggtc
    33601 gtaggattgt ttatggtaat tttagctaat aagattgcca aaaagctagg agaagacggc
    33661 gtttactaag atagaaagag agggaaagat atgctgcagt cattagataa aataaacgga
    33721 aggctaaatc gtattttaca gttggtttat ttgaattttc tgtggggcgg tttagtcatc
    33781 gcaggtttgg gagtttttac tttcggtcct gcatcgtatg cgatggttgc agtgattaga
    33841 aaatggctgc gctcaaaaga aacgtttcct ttgacgaaaa cgtacttcac atattttaaa
    33901 gaaaactatc gtgaaacctt gctgatgagc tggatatatg gcgccatcgg gattgtttta
    33961 cttgtggatc tttctttcat acacaattgg tatcttagga tcgctttgta tattgctgcg
    34021 ttcatatatg tgctctcagc actctatatt ttcccaatca tggctcatta tgattggaaa
    34081 ggatattttt tcaaaatgaa aatggcattt ttattcggat tctcacatct tcattactca
    34141 ttagtgttga tattaagtat ggtagggatt tatctgatga tcatccaaac aatgccaggt
    34201 gttttaacct ttatcggtgg cagtctcttc ttttccgcag tcacgtggac agcaaattct
    34261 atattttgcc atttggaatg gaagaactca tatacagaaa aaacagaaga aaacaaaaat
    34321 attattaaaa cagtgaagga gaaaatttat gaaaaaagca acgctcgtaa aattaggtct
    34381 tagttttgca ggaattctag cacttgccgc atgcggcagc agttcaagta acagttcttc
    34441 agagacagaa gatggaaaag ctaaaatcac ttggttaaat attttgcatc atgcttcacc
    34501 gcctacagac acgattgtga acttgatcga agaaaaaacc gatacggaac tgacattcaa
    34561 ctggattcct gatgcttcaa aagaagaaag attaacaact gcattggctt ctgatgaact
    34621 aggagatatc gttacacttc aaattgcgat gttgaaaaac tcttctgtcc gcaatgcgtt
    34681 aaaatcaggc gtattctggg atgtatcaga atatttaaaa gactataaaa atttaagtca
    34741 aatttctgaa gatagaatcg aagctgcgag tatcgacggt gccttgtacg gcgtaccgat
    34801 acaaaaagac tacgctcgtg ctggcttagt cattcgaaaa gactggttgg ataatctagg
    34861 actagatgtt ccaactacca tggatgagct ttatgaagta gctaaacaat ttacagaaaa
    34921 cgatcctgat ggaaatggtg tggacgacac agttggtttt ggcgatcgtg cgcctagtga
    34981 tattcgttat accagcttta aacttttcac ttcttatttt ggagcaccaa atggctgggc
    35041 tgtagacgat aatggcaaat tcactccaga atttgataca gaagagtata tgaaagcttt
    35101 gaatttttca agagatctat ttgagaatgg ctatttggca caagactttg cagttaccca
    35161 aaagactgat cagcaagaac aatttgcaca aggaagaact ggaatctaca ctggaatggt
    35221 ggatatcaag aatctgaaga cgatgggtca aggtattcaa gatgatatgg aattagtgcc
    35281 tgtaaacaag atctcagatg gtgaaggcgg cggttatcat gtttggtcag aaggaaatgg
    35341 tattggcggc ttgctagcat ttcccaaatc tcaagtaaaa gacgaagcac aattgaagaa
    35401 attacttcaa tttgttgatg atcttttaga agaagatgtg tataaattga tgactggcgg
    35461 tatcgaagga acgcattatg aattgaatga tgatggttct atcgaattta tcgatatgga
    35521 tctttggcaa caagaggtgc aaccgttatc aagctctcgt cctagtgaag tgacgttctc
    35581 tttcaaagat acagaccctg aaaaagaatt ctctaatcaa ttgatcaaag aaaatgaaga
    35641 atttgctgta atggatcctt ctgtgccatt ggattcaccg acgaacaatg aagtaggatc
    35701 agaaattgaa aaaatcgtca ttgatgctac ggtgcaatac atcatgggac agcttgacga
    35761 agatggattc aaaaaagctg tagaagactg gaaagcacaa ggaggcgatc gaatcacaca
    35821 agagtacgag gaagcataca aagcagcggc acagtaatat agacgattgg gacaaatact
    35881 tcttggtatt tgtcccaaat cctattttaa tttttaaagg agagcaaata atggaaaact
    35941 ggtcaaagaa attagcaaaa agtatcatgg aaagaacacc tcgcttatat gaagaaaagt
    36001 ggtataaagg aaaatggtct tatgactatg gcgtcgtact taaagggttc caattacttt
    36061 gggaacgaac ccaagaaaaa atttattttg atttcataaa agacaatatc gactatttcg
    36121 tgcaagaaga tggaacgatc cgtggctaca gcgttgaaga atacaatatc gaccatgtaa
    36181 atacaggaaa gcttttcttt ttattgtaca aagaaacagg cgaagaaaaa tataaaaaag
    36241 cagcagaact cctttcccag cagttggcaa accatccacg cacatccgaa ggagcttttt
    36301 ggcataaaga aatttatcct tatcaaattt ggttggatgg cttatatatg ggttctcctt
    36361 tttatgctga atttatccag aattttagtg aagaaaaaga ttaccaagat gttttgagac
    36421 agtttgaaat cagttataaa catctattcg atccaaaaac aaatttgctg attcatgcat
    36481 gggatgaaaa gaaggtccaa ccatgggctg atccaaaaac aggtctatcc catcattttt
    36541 ggagccgatc gattggctgg tatttgatgg ctgcagcaga tacctatgaa ttacttcatg
    36601 aagaaacaga ttgttctttg ctgaaagaaa ttctagaaaa gacactggct gcattacttc
    36661 catatcaaga agaaaacagc gggacatggt atcaagtaac aacagaaaca aaacggaaag
    36721 gaaattatct ggaagcttcc ggcagtagta tgtttgttta cgcaatggct aaaggaatac
    36781 gtttgggact gctgaacaaa gaagaatggc ttccacaagt gaagaaatca catcaaggat
    36841 tacttgatga atttgttctg gaaacaaaag aaggctggct gaacttgaat aaaaactgtc
    36901 aagtagcagg gttaggcgga gcagatcagc gagatggttc atatgcttac tacatcagtg
    36961 aaccaattat ttgtaatgat caaaaaggcg ttggtgcatt cttgcaagca ttgattgaag
    37021 tggaggcatt ataaatatgt atgatatttt gacatttggc gcatcaattg atgaattgaa
    37081 tactgaagca atacaacaag ccattgatgc tgcagcaagt gacggaggcg ggaccgtagt
    37141 tgttccagca ggtgaatttc taacaggagc tttattttta aaaagcaatg tcgaacttca
    37201 tttaagcgca ggagcggttc tgaaattctc tgacgatcca aaagattatc ctgttgttca
    37261 ttcacgttgg gaaggagtcc atcgaaaagt ttatgcttcc tgtatctatg ctcaaaatgt
    37321 agagaacatc tctgtcactg gtttcggtac attagatgga aatggaaaaa aatggtggca
    37381 tacttttaga aatgaaccag ataatctcgc ttatcctcgt cctaaattga tgagtttcca
    37441 taactgtcat cgaattactg tgaaagatat taaattgatc cagtcaccaa gttggacgat
    37501 caacccgatt ctttgcagta atgcaacgtt tgataacttg acgatcttga acccagcgga
    37561 ttcaccaaat acagacggaa ttgatccaga atcatgcaaa aatgtgcgta ttagtaattg
    37621 ccatattgac gtgggagatg actgtattgc aatcaaggca gggactgaag atacgtacga
    37681 aaggatcgct tgcgaaaata tcacgatcac aaattgcacg atggtccatg gacatggcgg
    37741 tgtcgtatta ggaagtgaaa tgagtggtag tattcgtaat attaccattt caaattgtat
    37801 cttccaagaa actgatcgag gaatacgatt gaaatctaga cgaggtcgag gcgggatcgt
    37861 tgaagatatt cgagtatcca atatagtcat ggataatgtg atgtgtccat ttatactgaa
    37921 tctttattat ttctgtggtc cgcgagggaa agaaccgtat gtatgggaaa aaaaggctta
    37981 tccgatagat gaacggacac cagcattcag aaggatccat ttttcgaata tcacggcaag
    38041 gaatgtccat gcttctgcag gttttattta tggattagcg gaacaattca tacaagaaat
    38101 cacatttgac aatatcgatg tctcgatggc ccaagatgca gttcccggtg ttcctgctat
    38161 gatgacagga atagaggaaa tgagcagaca aggattttat attggctttg cgggtaaggt
    38221 ttcctttaac cagatgtcga ttgaaaatca cgagggacca gcatttctat tagaaaatac
    38281 taaagaaatc agtattgaga aattccaatc aaatcaatca gaaaatatgg atgaactggt
    38341 tgttcgtaaa aactttgctt atgcagttga atgatttttt aggtgctcca tctagcagac
    38401 atacccctag tggaaaacga ttataccgag aaaaacaaaa cggttttatg cttgaatact
    38461 atcaattgta cttaaatgaa ttggaaacag taccagccct ctatgcttat ccagaaaaag
    38521 aaggaccgtt tccgccggtc ctttacctgc attctcacgg cggagatttt tcagtcggga
    38581 aaagtgaatt gatccatggt gctccttact tagcttctcc atcttttgca gaagtactga
    38641 caggaatggg atatgctgtt tggtcgattg atgcttgggg atttgaagaa cgcggaggta
    38701 tttctgaaag cgagttgttc aaacaatttt tattaacagg aaaaacgtta tggggaatgc
    38761 gtatttatga tgtgatcagc ttgatcgatt atttggagac aagggaagac acagatactc
    38821 aacggctcgc aacgattggt ctttctatgg gaggcttatt aagctggtgg gtgacagcat
    38881 tggattctcg tgtcaaagtt tgtattgatc tagcggcaca ggtggacatc gaaatacttt
    38941 tgctacatcg ccgcctagat catcatgggt tttattacta tgtgccaaat ctattgacag
    39001 cttatacaac gttagcaatc caagaaaaaa tcgctccgcg ccctcgtctt agcttagttg
    39061 gaaaaaacga tacgatgtgc ttggacgaag gcgtactgaa gctgagaaaa gggctggaca
    39121 aaaagtatca atcaatgggt agtccagaaa acttttcttc tttttcttta acaggcggtc
    39181 atatggaaac acaagaaatg agaaatattt ggaaacaatt cataagggag catttatgag
    39241 gatgaaagac aaactgataa ttggaaagaa tgctatagat accccgacgg attttgaaac
    39301 tatccaagaa ggaatcgatt atttggcagg cctgccggat agccatccaa aggtcttgat
    39361 agtaaaagaa ggaagatatg atgaatatat agaaagcaga ttatccaatt ttaaacttgt
    39421 gggagcagga acagtagtta tctccggtaa tcgttttgca aaacaggagt atcttgacgg
    39481 tagccagtta gggactttcc gaagtgctac ttttttctta gaaggatcga atgtcgtatt
    39541 agaaaatctt cacatcgaaa atacagcagg tccaggagaa aaggtaggac aggctgttgc
    39601 tttattcaat tcaggagatc gcgtatcttt aaaaaactgt cgactatctg gttatcagga
    39661 taccttatgc acgggtccgt tgcctgattt gcaaaaagat ggtactcctt tttgtacacc
    39721 ggcttctgcc aatcctgagt attgtcgtca gacctatgaa tattgctgga ttgaaggaac
    39781 ggtggatttc atatttggtg gggcagatgc cgtttttcat cagtgtcata tccatagctt
    39841 agccagtaaa aatcctggat acatcacagc agcatctacg ccgaaaaatc aaaaaacagg
    39901 gtatcgtttt gaaagctgta ccttgactgc agaacctgat actagaaacg tctatttagg
    39961 aagaccgtgg cgtccgtatg caaatgtctt atttgaagcg tgtaagatag gtgggcatgt
    40021 tcacccaact ggttgggata attgggatga caagaaaaat gaacagactg tcaaattctc
    40081 tgaacgtaat aacaattatg aaacatcgat caaaagacct gaatggatac atttagaaaa
    40141 gcagggggtt cagaaatgaa aagaaaatgg gaactattgc taggaatggt cggtggcagt
    40201 ctatctttga ttttctttgg cgggttagca gttactttaa gtaatatgtc tgcgtcagag
    40261 tttaaaaaaa gttatcagtc gcttgctgta gatcattcaa ctttgtcttt agaaaataca
    40321 tttggattat tacaggacat gactggtctg tttgccgttg tgttgtttat ttcacttgct
    40381 tttttagcag tagcattgtt tttgactgct aaaggaaaat acttaacaac tgccactggt
    40441 ttgtatttta taacaggatt catattgttg attggcactc agtttattgc gtttcctttt
    40501 gcattttttt atttcgcagc tggggcattc agtttatatc gagtaagaat gagaaaagga
    40561 gcctgaatca tgcagactat caaagtgaaa aatcctattt taagaggatt caatccagat
    40621 cctaatatat taagagtgaa agagcggtat tacattattg tctcctcttt tgaatggctt
    40681 ccaggaatcc gagtatatga gtcgaaaaat ttagtcaact ggtcacatac aacggatatt
    40741 ttgaccactc aagtatcttt gcaaggaaat cctacaaatg gaagtatatg ggcgccacaa
    40801 atcagttatg ctgatggatt gttttatctt gtttacacag atgtcaaaag tgcaggccgt
    40861 ccgtttaaag atgtccacaa ttatttgatc actgcatctg agatcacagg tccttggtcc
    40921 gaaccaattt atttaaatag taacggcttt gatccttctt tgttccatga tcttgaaacg
    40981 ggaaaaaaat ggcttgtcaa tgaactatgg gattatcgtc tggacacacc gaataaatcc
    41041 agcggtatcg tattacaaga atatgatgca gagaagaaga agcttgatgg tcccatctat
    41101 aaaatatttg caggtacaga attagcgaaa acggaggcac cacatttgta ttttcgtaac
    41161 gggtattatt atttgatgac ggcagaaggc ggtacaggaa gaggacattc agttacagtt
    41221 tgccgagcaa agaatattac tggtccttac gagcttgatc ctggttttcc tatgctgaca
    41281 gctagtgaca aacctgcttc tacattgcaa tgtacaggac atgggagctt agtacaaacc
    41341 ccgtcaggaa gatggtacat gacatatctt tgtaccagac cattaaacgg tgcggctatt
    41401 ttgggaagag aaaccgctat tcaagaagtg tattggacgt ccgacgattg gttaagactt
    41461 aaagatagcc agacaactcc gatggaagaa ataatgattg agacaaatga agaggtacaa
    41521 caagaaaaag atcatcagtt tatggataca tttgaaggag agctaaagaa gaactggaat
    41581 gccagacgca ttttgccaaa tgctgattgg tgtgatacaa aatgtagacc aggtttttta
    41641 cgtctgattt ccggtgaatc tcttcaatct accttcgaac atcatttgtt agctattcgg
    41701 caaacggatt ttacttttga tgcggagaca gcatttgaat acatgcctaa taattttaat
    41761 cagatggctg gactggtact gtacttaaat gaatccaatt atatttattg ttatgttact
    41821 tggaatgagg acaagggaaa atgcctacgt atgatagaat cagaaaacgg agtagcacaa
    41881 atagaagatt ggacgattcc actcacagaa aagaagactt ttttgaaagt cactgtaaag
    41941 aaagagacag cacagtttca tttgaaagac gtatcaactg aagaatggaa gaaagtaggt
    42001 ccgcaaaaaa acatgtgtat actctcagga gggtttactg gaaattttat cgggatagca
    42061 gtgcatgatc taaataaaaa aagaggaagt tatgctgatt ttgagttttt caagtatgat
    42121 ggaaaagatc atctgtaatt taattcttta ataaatgagg ttcactaact tattttcact
    42181 agttagtgaa tttttttgtt tgtatatatt gttatgtaaa aaaagttatc aatcttatgc
    42241 atcaatgttt ttagattatt ttttgttttt tatctgtcaa gataatgcaa taaaaagcca
    42301 acattgtgaa tataaaaaca agaaaaaaag agttataatt ttgaaaacgt ttactagaag
    42361 aggtaggagg aattcaaatg ctttcacaaa atgctgttac agcaaataca ataaaaaaag
    42421 aaaaaaagga atttgggatt tccgataaga taggctacat gttcggtgat ctaggtaact
    42481 gttttatttt aggtttagtc aatagtttct tgatgattta ttatacgaat gttcttggcg
    42541 tttcaggggc aatcgtaggt acgttattct tgatttcgag aattttggat gcgtttgctg
    42601 atgtgatggt tggaagatta tgtgatgtct cagagttaac agaaactggc cgatttattc
    42661 cttggatcag aaaaatgaag tatccatttt gcttagtgac tgtcattttg tttttgccat
    42721 tcgtccaaca tcttccaatg acggtgaaga ttgcttatat ttttgtttca tacattgttt
    42781 atgggatttt tctctcaaca atcaatatcc cgtatggagc aatggcagca gcaattagct
    42841 caaatcctaa tgaccgcacc tcgttgtcca cgttccgaag cattggttct gcgattggcg
    42901 gatcaactac aggattcttc attccgattt taatgtatac ccataacaag agtggacaac
    42961 aagtcgtttc agggaatcat ttcttttgga tttcaatcgg atgtgctgca atagcatttg
    43021 tcgcatatat gttaacttgt aaattgacta ctgaaagagt aagagttgtc aaaaacgaaa
    43081 aagtatctgc ttctcagttg gtaaaaggtt tgttagctaa tcgagcactg attattttag
    43141 tcattgttga cattttcatt gtgatcaatc agattttagc tgggacgaat atgacttatt
    43201 tattcaatga ttacttccat aataaacaag caatgtctgt tgctctttta tttacttacg
    43261 gaacagtagt agttttagca ccatttgcta caaagctgac aaaattattt ggtaaaaagg
    43321 aagcaagcgt atttgcgtta cttgcttctt catgtatgta cttgctgatg tatttccttc
    43381 atataacaaa tgcatgggta tatctgattc ttttatttat cgctacttta ggatctggtt
    43441 tattcaattt gatggtctgg gcattcatta ctgacgtgat tgattatcat caatataaga
    43501 caggcttaag agaagatgga acagtatatg gagtaaattc ttttgcgaga aaagtaggac
    43561 aagcatgtgc aggagcggta ggcggattta tgctggcact cattggttac caatcgtcta
    43621 caacaggtgg agccatccaa tcagttttag tacaagaaag gatttatacg atggcaaact
    43681 tgttaccagc attctgctta tttgtatccg caatcatttt gctagtaggt tacccactga
    43741 atcgttcaaa aactgtattt attggtgaag aattgaaaaa aatcaatgaa agatagggag
    43801 aaacgtattg cggacaaaaa aagggattat caatgctttg aactttaaaa caaacgacac
    43861 tcaacaagat gatacaataa tactgcaaaa agcaatcgat caaggtgcga aagaacactt
    43921 acctgtattt attccaaaag gcatttattt agtcggagcc ctttttttaa aagataagag
    43981 tcatcttatc tttgaagaag gtgcagtcct caaggggaga acagatatcg aagcatttcc
    44041 agaaatcgat acacgagtag ccggcgtaga aatgaaatgg ccagcagcga ttttgaatgt
    44101 tctctcggcg aaagatattc ttatagaagg aaaaggaata atcgacggtc aaggagacca
    44161 ttggtgggag ctgtattggg gaaaagatca aaaaagcggg acaagagctg aatacgatca
    44221 aaagggactt cgttggattg cggattatgc tattaaacgt ccacgcgctt gtttactgta
    44281 tcatgcagaa catgttgttg ttcgtgattt gatttttcag aaatcaggtt tttggaactt
    44341 gcaaatcact tattctaacg atgtattagt agaaaaagta atcatccgca acaatgatgg
    44401 accaagtaca gatgggattg atattgattc ttcaacaaat gtgcgtgtct atgaatgtga
    44461 tttggcctgt ggtgatgatt gtattgctat taagtctggc agagatggca atggtgcaag
    44521 agtcaatcga aaatcatcaa gaatcgaggt tgctagatgt aaaatccgtt caggatacgg
    44581 tgtaactatt ggtagcgaag ttagtgctgg agttagtgac gtatatattc acgatatcga
    44641 tttttttcag tcagactgtg gtattcgaat gaaatcttca aaagaaagag ggggagtgat
    44701 cgaaaatatc agggtagaaa atctgaatat gattgatgta caatttcctt tttcttggat
    44761 aatggactgg cataatgaat acaatagaaa aaatagcaat gaccttgctc aaatgccaga
    44821 aagttggcaa gcagtggctg aagaaatacc tgaatcaaag caaatgacga aagtcaaaga
    44881 catacacatc aaaaacgtac aggcatgcct ttctccaggt tactcacttc cttcccgagc
    44941 ttttgattta gtagcatttc ctgaaaagcc tatagaagat gtctgctttt ctcattgcca
    45001 aataactgca aaagaattcg ggcgaatcga agcagtacaa aacttatgtt tcaaaagctg
    45061 tgatttatct atagaaactg gcaacacagc tgcaaacaat acttttgaca atcgttgaaa
    45121 ttataataag gtcaagctta aataatattt tagcttgacc tttatttttt ttaatgatat
    45181 attaaacaac gttgtcgcgc ttcattgaaa cattttatga aaattccaaa aaagagttga
    45241 cacatgtttt tagacgtgat aagataacta agtcgt
//
LOCUS       DAYYCM010000016        36321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000016 DAYYCM010000000
VERSION     DAYYCM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.016"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            904..1218
                     /locus_tag="ACHAY1_000986"
     CDS             904..1218
                     /locus_tag="ACHAY1_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8592999.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1271..2044)
                     /locus_tag="ACHAY1_000987"
     CDS             complement(1271..2044)
                     /locus_tag="ACHAY1_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593000.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2149..2394
                     /locus_tag="ACHAY1_000988"
     CDS             2149..2394
                     /locus_tag="ACHAY1_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8593001.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2550..3401
                     /locus_tag="ACHAY1_000989"
     CDS             2550..3401
                     /locus_tag="ACHAY1_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8593002.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3434..4111
                     /locus_tag="ACHAY1_000990"
     CDS             3434..4111
                     /locus_tag="ACHAY1_000990"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8593003.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            4599..5564
                     /locus_tag="ACHAY1_000991"
     CDS             4599..5564
                     /locus_tag="ACHAY1_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8593004.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5586..5798
                     /locus_tag="ACHAY1_000992"
     CDS             5586..5798
                     /locus_tag="ACHAY1_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8593005.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5873..6349
                     /locus_tag="ACHAY1_000993"
     CDS             5873..6349
                     /locus_tag="ACHAY1_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8593006.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6346..8154
                     /locus_tag="ACHAY1_000994"
     CDS             6346..8154
                     /locus_tag="ACHAY1_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8593007.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8231..8446
                     /locus_tag="ACHAY1_000995"
     CDS             8231..8446
                     /locus_tag="ACHAY1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8593008.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8538..9401
                     /locus_tag="ACHAY1_000996"
     CDS             8538..9401
                     /locus_tag="ACHAY1_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8593009.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9438..9974
                     /locus_tag="ACHAY1_000997"
     CDS             9438..9974
                     /locus_tag="ACHAY1_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8593010.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            9988..10410
                     /locus_tag="ACHAY1_000998"
     CDS             9988..10410
                     /locus_tag="ACHAY1_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8593011.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10340..12739
                     /locus_tag="ACHAY1_000999"
     CDS             10340..12739
                     /locus_tag="ACHAY1_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8593012.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12771..14762
                     /locus_tag="ACHAY1_001000"
     CDS             12771..14762
                     /locus_tag="ACHAY1_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8593013.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14785..15036
                     /locus_tag="ACHAY1_001001"
     CDS             14785..15036
                     /locus_tag="ACHAY1_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8593014.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15026..16255
                     /locus_tag="ACHAY1_001002"
     CDS             15026..16255
                     /locus_tag="ACHAY1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8593015.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16252..18333
                     /locus_tag="ACHAY1_001003"
     CDS             16252..18333
                     /locus_tag="ACHAY1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8593016.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18482..22402
                     /locus_tag="ACHAY1_001004"
     CDS             18482..22402
                     /locus_tag="ACHAY1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8593017.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22403..23347
                     /locus_tag="ACHAY1_001005"
     CDS             22403..23347
                     /locus_tag="ACHAY1_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593018.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23351..23554
                     /locus_tag="ACHAY1_001006"
     CDS             23351..23554
                     /locus_tag="ACHAY1_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8593019.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23854..24090)
                     /locus_tag="ACHAY1_001007"
     CDS             complement(23854..24090)
                     /locus_tag="ACHAY1_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593020.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24273..24644)
                     /locus_tag="ACHAY1_001008"
     CDS             complement(24273..24644)
                     /locus_tag="ACHAY1_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8593021.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24641..25969)
                     /locus_tag="ACHAY1_001009"
     CDS             complement(24641..25969)
                     /locus_tag="ACHAY1_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8593022.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25930..26259)
                     /gene="mobC"
                     /locus_tag="ACHAY1_001010"
     CDS             complement(25930..26259)
                     /gene="mobC"
                     /locus_tag="ACHAY1_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8593023.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26517..26887)
                     /locus_tag="ACHAY1_001011"
                     /pseudo
     CDS             complement(26517..26887)
                     /locus_tag="ACHAY1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27032..27217
                     /locus_tag="ACHAY1_001012"
     CDS             27032..27217
                     /locus_tag="ACHAY1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8593024.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27375..27542
                     /locus_tag="ACHAY1_001013"
     CDS             27375..27542
                     /locus_tag="ACHAY1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593025.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28160..28819
                     /gene="vanR-B"
                     /locus_tag="ACHAY1_001014"
     CDS             28160..28819
                     /gene="vanR-B"
                     /locus_tag="ACHAY1_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8593026.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28822..30183
                     /gene="vanS-B"
                     /locus_tag="ACHAY1_001015"
     CDS             28822..30183
                     /gene="vanS-B"
                     /locus_tag="ACHAY1_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8593027.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(30274..31575)
                     /locus_tag="ACHAY1_001016"
     CDS             complement(30274..31575)
                     /locus_tag="ACHAY1_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8593028.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            31854..32660
                     /locus_tag="ACHAY1_001017"
     CDS             31854..32660
                     /locus_tag="ACHAY1_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8593029.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            32678..33385
                     /locus_tag="ACHAY1_001018"
     CDS             32678..33385
                     /locus_tag="ACHAY1_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8593030.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            33382..34353
                     /locus_tag="ACHAY1_001019"
     CDS             33382..34353
                     /locus_tag="ACHAY1_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8593031.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            34346..35374
                     /gene="vanB"
                     /locus_tag="ACHAY1_001020"
     CDS             34346..35374
                     /gene="vanB"
                     /locus_tag="ACHAY1_001020"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8593032.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            35380..35988
                     /locus_tag="ACHAY1_001021"
     CDS             35380..35988
                     /locus_tag="ACHAY1_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8593033.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(36054..36227)
                     /locus_tag="ACHAY1_001022"
     CDS             complement(36054..36227)
                     /locus_tag="ACHAY1_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593034.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9710 a         8746 c         9388 g         8477 t
ORIGIN      
        1 agcggaatat aaagaaaaga gaaaagtaat taaacgcaaa aaaatgtaga acttaaaaaa
       61 tataaagagg aacaaccgtt aaaattagga atgatgttgt gtaaacatta taattgctat
      121 gatataaatg aagtgttaaa gaaagcaaat ttaaaccaat tttgtgaaca gaaaagcctt
      181 agtcactatg accaaaacta cggacgaaac tatgaccaaa agtaggtact aaattatgac
      241 cgaaaatagg tccaaaatgg atttttttat gatatcaaaa attatgaaat gattttgaaa
      301 gaaaaccagt tggcacagcc aacttagcca ccttcctgta ggaaggtgct atcagttttc
      361 ccttatgctc ttttctttct tttttgtttc atatgtcaaa tgccattttg aaatgtacca
      421 tcttaagaaa tttatcctta aaaacaattg cgttattgga taaaaagatg ctacattatc
      481 ttaaaaaagg aatcaaatat gatgaattta gtggctagca tatcgtgtga ttaagatact
      541 ttatagtgta agttgaagga gaaaaaatat ttaaaatata cttcccattt cagtgggaag
      601 taaaattaat cttgttgttt ctactagtaa tgtatctttt tggaaactat gtaacgatgt
      661 aaacaagatt tttttcagaa gaaaaaacga taaaagttgt gataaacgat ttaaaaaata
      721 ggagaaaata tatcaagcgc atggtatgaa acgcacattt aaaaagattc gactaactaa
      781 aaaaataagt ttacaaagca ttgttgtgta ttacaagtga tctatacact gaaaaatgat
      841 agagaaaatc tgatacgtcc gcaacaatgg acaattgaaa atgaaagtga ggaaattgga
      901 agaatggctg atgaaaataa agttgttccg cttgaaacac tagtgataaa taaggaagag
      961 tttgagcaac tttttcttga tttttttgtt caatatgaca ggatttatga ttttagttcg
     1021 tcactagatt tatttgaatt agaggcagaa ataatagaaa acagtcgaga ggagaatggt
     1081 aaaatcatca tttcagtaga aaaattcaat gaattattct cagaaacatt tgaaatagat
     1141 acagattatt atcaaccaca agaagttttc caatcattta gaaatcagat tattcgctca
     1201 ggtaagaccg taaactgaaa aacaataaaa gaaacgttga aagaataatt attccttcaa
     1261 cgtttctttt ttagaatgtt ttaatgacca aagcagtttc attgtcaggc ttaggtaatt
     1321 catgaaatcc cgtatttata gtgctcaatt gatcaactaa ccatgtttta ctttttgaat
     1381 tgtagataaa tctaaaatta atatcgttat tatcgtgttg aaggggcgtc tccaaataat
     1441 ctgctgtata ttcatagtca acgtaacctg ctaatacaaa agagagtatc cctttattta
     1501 atgttagatt taatggataa tcatacgact ttattttttt caacttcata ttgtacatat
     1561 cttcagccat aaaagttgag ttcgtgattt catcttgggt ctcattaaaa aagttatccg
     1621 aataatcgct tatttttgaa aagtctttat tggcaccagc ctcaaaaaat tgttcaactt
     1681 tttcagtata aagttgtgct aacgctcctt tttcaatatt aactttaata ttttcatgtc
     1741 tatcaatttg tgtgctgttc agtaagggaa gatcagcttt tagttttata tcgtcttgat
     1801 ttctttcaat ttcaattggc aaaatctttt cagtagattc agtttctgat aaaggaacat
     1861 ctagtttttt atcatgataa taagcttcaa atccatctgg aaccggaaaa aaatgtattg
     1921 ggtaattctt ttctgaggtt gttgtactag tttccgtact tgtagaactt gtgtcagaag
     1981 tggtacaccc gcttatagtt ccgatgagta aagcgcaagt tagaatgatt gcaaatttct
     2041 tcaaaaaaat tcctccttaa aattgaatat ggactaatac tagtcccatt gtagcatacc
     2101 gaatctttaa gattaataag aaaaaggact aattttagtc caaaaggtgt gctattcttg
     2161 aaagaggaaa aaattattga taaagaaaat gtaatcggta gcaatattag acgagtccga
     2221 ttagaaaaag gaattggtca aaccgagctg attagagacc tacagcttcg taaaattgct
     2281 ataactaggg aaacgttggt taagattgaa ggtggccgcc aacacattaa gttggatcaa
     2341 ttgaaagcca tcaaagatat tctacaggtg tcttatgaag atttgcttca atgactactt
     2401 gtagtcattt cgtttgaaat tattatgtta cacccttatg tgtcacgaag aaatactggt
     2461 ataataatga atgggatatg taacgatagg gtatactgtt atgttgccta ataaattgtg
     2521 gatcaaaata caagaatgga gggatatcct tgtataaggc atatgaattg gattttacaa
     2581 atgctgactt aaatgcattc tcctcaagct cgcaatcata tacacatgta gtaaatcaaa
     2641 tagatcacaa ccaaaaatct attaacaaga gaatagaaaa tctatttgaa agtgaaaatg
     2701 atttagttga ttattattct aaggacagca aaattctaga tgcagacgaa attattaaag
     2761 attggtttcc aactataaaa gcagatattt ttatatctca ctctcatgct gatgaaaaat
     2821 tagctatacg ttttgcatca tggctatttg aaaattttgg tcttactgca tttattgatt
     2881 cttcggtttg gggatacagt tctgatttat taaaaaaaat agaccaaaaa tattgctata
     2941 aggaacaaac aaaaacatat gattatgata aaagaaatgt aacaactagt catgttcata
     3001 tgatgctttc tacagcttta aataatatga ttgatagtac agaatgtctg ttttttctta
     3061 atacacctaa ttcaattagt ttgagcaatg aaatcactaa tgaacagaaa tttacgtatt
     3121 cgccatggct atatagcgaa ctgacgacag cttcaatagt ggaaaaaaag aatccacgat
     3181 tggaaagtaa tcctcaaatg agcaccgagg atgttagaag cattataaag cactattctt
     3241 cgcaagaaaa tctgcaaata aaatattctt taaatttaag tgaattaact tatttacgaa
     3301 aagctgattt gaaaaaatgg gctttagatg ctgtggaatg cactaaagga agtaatacat
     3361 tagatatttt atataaaaac accattttca ctaatctcta atcaatttac tgaatcgaaa
     3421 ggaaccgggt agaatggata agaaagtttt aataaagagg ttctctgaag ctattgaaaa
     3481 aggaaatgca gcaatatttg caggtgcagg attatcgatg tcacaaggat atgtaagttg
     3541 gccggaacta cttaatgatc ctgcaacaga aatcgggttg gattctaaaa aagaaacaga
     3601 cttagttact ctggctcaat attataaaaa tgaaaatggt ggatcaagag gaattttgaa
     3661 tcagatactt atggataatt ttggtgaaga attagaaata tctgaaaatc atcgaatact
     3721 agcaagttta cctattgaaa cttattggac aactaattat gatcatctaa tagaaaaatc
     3781 aatccgagaa gcttataaaa accctcaggt taagaaaaat tatactcagt tagcaacaac
     3841 taatccaaat gtggatacaa ttgtttataa aatgcatggt gatatagatg atgtcagttc
     3901 gacagtaata acaagagatg attatgaaaa atatgatgat tcgtacgcgt tatttaaaga
     3961 aactttaaaa ggagatttgt taacaaaaac ttttctcttt ttaggattta gttttacaga
     4021 ccctaattta gaaagaattt tatcagatat aagatgggtt ttgagagaaa accaaagacc
     4081 tcattattgt ataatgagaa aaatattata gaaaaaattt taggttatcg ctgaaagccc
     4141 cggaaatacg ggcatttcgc aggatatgag aactcaattt actaccaatt tactactttc
     4201 ggattgagcc gcatatatgc tgaaccatac tgctaatacc cgccacccca aatacggcac
     4261 atcataaatt gaatacgaca ccgtaaacgc ggcgttcctc tttcccgtca tgggagaaca
     4321 ggacgccgct tttttatgcc caaatagaga aaggaggcga cgccatgcta tttacttccg
     4381 gccctgaccg ggcgtttgaa cggctgatgc aggaaaagcc cggtttcgat cactacgacg
     4441 gcggcggggc ggacgcgccg gaggattgcg gcacctgccg attttaccgc ccggattgga
     4501 aatatcaatt ctgccagttt gcagagtgtc cctatcagcc gggcaagctg accgactatg
     4561 cagcggtcaa attcacagtg aaaggagcga acaacgctat ggcagttttc cgtgtggaaa
     4621 agaacaaagg ctatacggtg atgtcaaatc accacctgcg gaataagaac ctgaccttaa
     4681 aggccaaggg cctgctgtcg cagatgttgt ccttgccgga ggattgggac tataccctaa
     4741 aaggactgtc ctatatcaac cgggaaagca tcgacgcgat caggacagcg gtatgggagc
     4801 ttgaaaaagc tggatatatc cggcgtgagc agggccgtga cgcaaaaggc aaaatggccg
     4861 atatggtcta taccatctac gaacagccgg tattggaaaa cccggtattg gaaaatccaa
     4921 catcggataa cccggtgttg gaaaatccga catcggataa tccggcgtcg gaaaatccaa
     4981 cgcaattaaa taaagaagta caaagaactg acttatcaaa aaaagaaaaa ataattacag
     5041 atggacaaag tacccattcc attcctatcc catccccgac ccctgcccct atggagggcg
     5101 aggcagcggc tacgccgccg gaacggaaaa ggacaggaaa ggatgaggct gtacggattt
     5161 atcgggaaat cattttggag aacatcgagt atgcctatct cattcaggac aggagcattg
     5221 accgtgaaca gcttgacgaa atcgtagacc tgatgctgga aaccgtctgc accgcccgaa
     5281 agacaatccg tgtcgccggg gacgactacc ctgccgagct ggtgaaatcc aagtacatga
     5341 agctgaccgg cgaacatatc cgctttgtcc ttgactgtct gcgggagaac acaacgaaag
     5401 tccgcaatat caaacaatac ctgcgggcta tgctgttcaa cgcccccagc accatttcca
     5461 actactacac ggcccttgtc gcgcacgaca tggcacagcc cgattgggga aagcctaaat
     5521 ccggcctgcc ggactattcc tgcggcgagg gcgaaagcct gtaacgagaa aaggaggacg
     5581 accctatgaa gcagggcgca ttgatttttg acgagcgaac agaccgttac gacatccgct
     5641 ttgatctggc cgactattac ggcggcctgc attgcgggga gtgtttcgac gtgatgatcg
     5701 gcggcaaatg gaggcccacc cgcattgaaa tggccgaaaa ctggtatctt gtcggcatcc
     5761 gggccgacga cctttccggc ctgcgggtgc ggatttgacc tatatctacc tgcggcgtct
     5821 ggtggttccg ccttagacgc cgcttttgac tttctgacaa ggagggatgc gattgcagga
     5881 agaagttact caaaagacca ttgccctgtc catgaaaacg ggcaagctca cggctcaagt
     5941 gctgcaagcg gcgctgaaaa agtacctgca acaccgggcc aagggcaaaa acacgctgca
     6001 ccacggccag cagagcctaa agcagcttaa aaagcatggg gctgatctct ccaacattga
     6061 gatcaccgag gccaacattg gagcgttcaa gccatgcgcc aagaaatacg gcgtggattt
     6121 taccttacga aaagataaga ccacccagcc gccccactac gttgtgattt tcaaggccaa
     6181 ggatgcagat aaccttgatc tggcgtttcg ggagtttacc gccaagacgc tttccaagga
     6241 gcagcggccc tctatccgca aggttttgtc cgctgtgaag caaaaggcgg cggcccagac
     6301 caagcagcgg gccaaggaga aaatcaagga aagggggctg gaactgtgaa gcctgacgta
     6361 aagaaattgc tgatcctgaa cctcccgtat ctgatttttg tgtacctgtt tgcgaaatgc
     6421 ggggaggcgt accggctggc cgctggtgct gacctgtcgg ggaagctcct gcattttgcg
     6481 gatggttttg cggcggcttt tgccaaccct ctgccaagcc tgcacctgtt tgacctgtgt
     6541 atcggtatcg ccggtgccgt gatcgtgcgg ctggtggtgt actgcaagag caagaacgtg
     6601 aagaaatacc gcaaaggcat ggagtatggc agcgcccgct gggggacggc caaagacata
     6661 gcgccttaca tcgaccccaa gtttgaaaat aatatcctgc tcacccaaac ggaacggctt
     6721 accatgaccg gcaggcccaa agaccccaag acagcgcgga ataaaaacgt gctggtgatc
     6781 ggtggcagcg gttcagggaa aacgagattt ttcgtgaaac ccaacctgat gcaatgcttt
     6841 ccgaccacgg actatccgac ttcgtttgtg gtgacagatc cgaaaggcac tttggttctg
     6901 gaaaccggca ggatgttcca gcaggcgggc taccgcatta aaatcctgaa taccattaac
     6961 ttttccaagt ccatgaagta caaccccttt gtctacatcc attcggaaaa ggacgtgcta
     7021 aagctggtaa ataccctgat ttccaacacc aagggcgagg gggaaaagtc agcggaggat
     7081 ttttgggtga agtcggaacg gttgttttat tcggccctga tcggctatat ctggtacgag
     7141 gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa tgataaacgc cagtgaagcc
     7201 cgcgaggacg acccggagtt tcaaagcccc gtcgatctca tgtttgagcg tttggagcag
     7261 aaagaccccg accattttgc ggtgcgccag tacaagaaat tcctgctgtc cgcaggcaag
     7321 acccgttctt ccatcctgat ttcatgcggt gccagacttg cgccctttga catccgggag
     7381 gtacgcgagc tgatggagga cgacgaaatg gagctggaca ccatcgggga cgaaaagacg
     7441 gtgctgtatc tcattatgag cgatacggac acgaccttta atttcattct ggctatgctc
     7501 caaagccagc taatcaacct gctttgtgac cgggctgatg atgtgtacgg cgggcggctg
     7561 cctgtccatg tacgcctgat tttggatgaa tttgccaaca ttggccaaat acccaatttc
     7621 gataaactca ttgccaccat tcgaagccgt gaaatatcgg cctctattat tttgcagagc
     7681 cagtcgcagc taaaagcgat ttacaaggac gccgccgaga tcatttcgga taactgcgat
     7741 agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg agatttccga tgcgctgggg
     7801 aaagaaacga ttgacagttt caacaccagc gagaaccggg gaagccaaac gtcccacgga
     7861 ttgaactatc aaaaattagg aaaggagttg atgtcccaag acgaaattgc cgtgatggac
     7921 ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt ttttatcgga gaaattcgat
     7981 attaccaagc atccccggta caaatacctt gccgacgccg acaagaagaa tacctttgac
     8041 gtggacagat ttttgactat cacgcgccgg aaacggcagc aagtggtaac gcaggatgaa
     8101 gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg ccgccgctga ataaacagcg
     8161 gcttttttca ttccaacaac aaaatcaaaa aatcattatg agccggaaac cggcagaaag
     8221 aggtaaattt atggcatttt ttaattccgc agttactact ttgcagacta tcgtgatcgg
     8281 actgggcggc gctctgtgcg tatggggcgg catcaatctg ttggagggat acgggcagga
     8341 caatccgggg gcaaaatcgc agggtgtcaa gcagcttgtc gctggcggcg gcgtggccct
     8401 gatcggtgtc actctggttc ccctgctgtc cgggctgctg ggctgatcct ggcctttgtc
     8461 cctgtccaaa cccttaacta atcgcctccg cgtcctcccg gccagaccgg gagggcgcgg
     8521 gaaaggaggt gctacccgtg attagtgaaa tcattgaaga atggataaaa ggcatcctca
     8581 tggacggtat cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc aaagtcggag
     8641 aaatcgccgc cgacgtggga cgaaccccgc aggcatggaa cagcggtatt ttcaatatgc
     8701 tgcacagcct gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc gcccttgtca
     8761 tgtgctatga gcttatccag atgataacgg aaaagaacaa tatgcacgac tttgacagct
     8821 ccatgttctt ccgctggata ttcaaatccg cgtttgcaat cctgatcgta acgaacacat
     8881 ggaacatcgt catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa agcgccggtg
     8941 tgattatcgg gcagacatcc attgatttta acaccctgat accagacctt gaaagccaat
     9001 tggaggctat gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt gttgtgggcc
     9061 tcaccatgaa tatcctttcc atctgcattt tccttgtgac ctatggccgc atggtggaaa
     9121 tctatgttgt cacgtcgctg gggcctatcc cccttgccac aatgggcaac gcggaatggc
     9181 gcggcatggg ccaagggtat ctgaaatccc tgctggcgct gggcttccaa gcctttttaa
     9241 tcatggttgt ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc gcttccgaca
     9301 tatccggcgc aatctggggc tgtatgggct atacggtgct gctgtgcttc tgcctgttca
     9361 agaccggcag tatatcaaag gccgtattcg gggcgcattg atcccaaaat ccaagcatac
     9421 acaggaggaa cttgtttatt tcaacgtaca acatcattta cgctgacccg ccgtggcggt
     9481 atgactgcaa aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg agcattgacg
     9541 agctgtgcgc cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg tttttgtggg
     9601 ccacattccc catgctgccg gaagctctgc ggttaatcaa agcgtggggc ttttcattca
     9661 agaccgtggc ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg ttttacgggc
     9721 tgggccgctg gacaagggga aacgcggaaa tctgcctgct ggcaacacac ggcaagccga
     9781 agcgtcgttc tgcctctgtc catcagttta ttatcagccc gattgaacaa cacagcaaaa
     9841 agccggatgt gacccgtgaa aaaatcgtag agctggcggg cgatttgccc cgcgtggagc
     9901 tgtttgccag acagaaaacc cccggctggg acgtgtgggg gaacgaggtg gaatgtgacc
     9961 ttatcctgac gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa ggacttaacc
    10021 catgtgaaaa gcaaggttgt gtttgggctt accaaacggc aactgatctg tttttccggg
    10081 gcgctggtga tcggcgtccc cttatatttt ctcacccgtg actatcttac caacagcgcg
    10141 gcggccctgc tcatggtatt tgccatgctg ccgggctttt tgcttgccat gtacgagcgt
    10201 cacggccagc cccttgaagt ggtgattatg cagatcatcc agtgctgctt catccagcca
    10261 aaggagcgtc cctatcagac cgacaacacc tacaccgccc ttgtgcggca ataccagatg
    10321 gaacaggagg taaaggccat tgtccaaaaa ggaaatccca agagaaaccg aaaaaacaaa
    10381 gctgacccgc gcccagcgaa aagaaattga cgcggtaatc cgtaaatata agggcgacgg
    10441 caggccccac acggcgcagc agagcatccc ctacgaggtc atgtacccgg acggcgtgtg
    10501 ccgggtatcg cccggcgtct tttccaagtg cattgagttt gcggacatca gctatcagct
    10561 tgcccagccg gacacgcaga cggctatctt tgaaaaactg tgcgacctgt ataactatgt
    10621 ggacgcctcc attcatatcc agttttcttt cctcaatcgg aaagttgacc cggtgcagta
    10681 cgccaagagc tttgagatcg cgccgcaggg ggacgacttc gacgacatcc gggccgagta
    10741 caccggcatt ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa agaccaaata
    10801 cctgacattc accatcgagg ccgaaagcgt gaaagcggcg cgagccagat tgaagcgcat
    10861 cggctttgac ctgttaggct atttcaagag catgggggcc gtagcccatg tgatggacgg
    10921 ctgggaacgg ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga tattcaattt
    10981 tgactggaag tggctggccc cgtccggcct ctccaccaag gattttattg ccccgtcctc
    11041 cctgtgcttc ggcaatgcaa agacattcgg catgggcggc aaatacggcg cggtaagttt
    11101 cctgcaaatc ctgtccccgg agctgtcgga cgatatgctg gccgacttcc tcaatacgga
    11161 aagcggtgtt ttagtcaatc tccatgtgca ggccattgaa cagaccaagg cgatcaagac
    11221 catcaagcgc aaaattaccg accttgacgc tatgaagatt gcggagcaga aaaaggccgt
    11281 ccgcagcggt tacgatatgg acattcttcc cagcgacctt gccacctacg gcgaggacgc
    11341 caagaagctg ctgacaaagt tgcagacccg caatgaacgg ctgttccagt tgacgttcct
    11401 tgtcctcaac gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc aagccgcagg
    11461 cgtggcccaa aagcataact gtccccttgt ccggctggac taccagcagg aacagggcct
    11521 tgcaagcagc ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc ttacgacttc
    11581 cagcgtggcc gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg gcgcggccat
    11641 gtattacggc atcaacgcca agtcccgcaa catgattatg ctggacagga agcaggcccg
    11701 ttgccccaat gccttgaagc tcggcacccc cggcagcggc aaatccatga gctgtaaatc
    11761 cgagatcgtc agtgtgttct taacgacgcc tgacgatatt tttatttcag acccggaggc
    11821 cgaatattac ccccttgtca aacggctcca cggacaggta atccggctgt cgcctaccag
    11881 caaggacttt gtgaacccgc tggacatcaa cctgaattat tcggaggacg ataacccgct
    11941 ggccctgaaa tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg gcaagaacgg
    12001 gctggaagcc attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct accggcccta
    12061 tctggcagac ccccgcccgg agaatatgcc gatcctgtcc gacctccaca aagcgttgtt
    12121 agatcagcat gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt atgtgtccgg
    12181 ctccctgaac gtatttaacc accgtaccaa cgtggacatc ggcaaccggc ttgtgtcctt
    12241 tgacattaag gagctgggca agcagctcaa aaagctgggg atgctcattg tccaagacca
    12301 gatatggggg cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt attttgccga
    12361 cgagttccat ttgcttttga aagaggaaca gacggcggcc tattctgcgg agatttggaa
    12421 gcggtttagg aaatggggcg gcattccaac aggtgccacc cagaacgtca aagacctgct
    12481 ttcctccccg gaaattgaaa atatccttga aaacagcgac ttcattacgc tgctcaatca
    12541 ggcgtccggg gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg aacagcagaa
    12601 gtacatcgac aattccgaac ccggcgaggg ccttttgatc tttgaaaacg tggtgctgcc
    12661 ctttaccaac cccatcccgc acaacaccca gctttacaag attatgacga cccgcttaaa
    12721 cgaggtggcg ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc atgaaagagc
    12781 tgaaagccaa ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg ctggaaaatc
    12841 aggccaccgg cgaggtggag aacatttcca gccgggaggc cgaacaggat ttgtccgcgc
    12901 cggggccggg cggcaccgcc gataagctgc tggaacgggc cgacgccatc catgacggcc
    12961 ataaggccaa gaaagccgcg aagcaggccg gggctgtggc cgccgagggg gacgctgccc
    13021 tgcaccggcc atccgcccgg ctccaactgt cggaggaaga acgggccgac ccggatttac
    13081 agccctatat ccgaaaatcg gacaaacggg ccgataagct ggataaggcc cgcgccgcca
    13141 tccccaagaa gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc ggcaaagcaa
    13201 agaccgtcct gcactttgac aaacaggaca aggggcctcc ctccctgaaa cccaatccgg
    13261 caggccgtcc tgtttccgag gtttttctat tcggccacgg gaaaatccat gaagtcgagc
    13321 atgaaaacgt gggtgtggag ggcggccata agggggagga acttgccgag cggcaggccg
    13381 gaaaagccat ccgcagcacc atccggcacc acaggctgaa accctactgg gacgcggaaa
    13441 aggccgagcg caggctattg tctgccaacg cggagtattt ctaccagaaa tctttgcggg
    13501 ataacccgga gatcgcacag gcggccagca accccatttc ccggttctgg cagaaacagc
    13561 agatcaagcg ccagtacgcc aaggccgccc ggacagcggg ccagaccgca ggcgcggcgg
    13621 cacagggcgc ggccaagaca gccggggcct ccacgaaagc ggccaaggcc gcagccaaag
    13681 aaagtgaacg ggcggcctcc tttgtggccc gccactggaa aggggcgctg gccgtccttg
    13741 tgttggggct tttgatgctg cttatcatcg gcggcttaca gtcctgcacc gccatgttcg
    13801 gcagcgccgg aagcggcatc acggcgtcct cttacttttc cgaggatagc gatatgttgg
    13861 ccgccgagga cgcctacgcg gagctggaag ccgagctgca agactatctg gacaactacg
    13921 aggccaccca tgactatgac gaataccatt ttgaccttga cgagatcagc catgacccct
    13981 atgtgctgat ttccattctg tcggcgctcc atgacgggat atttaccatc ggggaggtac
    14041 agggtgattt gtccatgctc tttgacaagc aatatatcct gacagaaaac gtcgtggtag
    14101 aaacccgtta ccgcaccgaa accgatacat ggacggacgc ggagggcaac acccatacag
    14161 acacctacca agtgccgtat gactattaca tctgctacgt cacgctggaa aacttcgacc
    14221 tgtcccatgt gccagtctat atcatggacg aggaaacgct aagtttctat tccctgtata
    14281 tgtccacgct aggaaaccgg cctgatctgt tttccggcag ccagtacccc aacgcctcca
    14341 ccctgacgcc gccgacctac tacgacgtac ccccggaggc tttggaggat gccacctttg
    14401 ccgccatgct caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg ggcggcagct
    14461 ctcccagcac cagctttgat tgcagcggct tcatttcgtg ggtaatcaat catagtggat
    14521 ggaacgtggg acggctgggg gcgcagggcc tatataacat ctgcacccct gtttccagtt
    14581 cgcaggccaa gcccggcgac cttgtatttt tcaagggaac ctacgacacc cccggcgtgt
    14641 cccacgtcgg cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac ccgatttcct
    14701 acacgaacct tacttcaaac tactggcaat cccattttta cagttacggg cgtttacctt
    14761 aacgccagaa aggagctttc aattatggca accaaacttg accgcatcga aaaggacatc
    14821 cagaaaacca agtctaagat cgcggagttt cagaaacagc ttcgggagct ggaaacgcag
    14881 aaaaccgaac aggagaattt gcagatcatc cagcttgtgc gcggcatgaa catgaagccg
    14941 gaggaatttg ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc cgcaacccct
    15001 taccatgaac aggaggacaa tgcccatgaa gaataagacc agacggattt cccgtattct
    15061 ccccgcactt ctggccgtga tgctctgcgt cacggccttt cccgttaccg cccttgcagg
    15121 cggcaatgac ccggcaccgg ccccgctgcc ggaggctaca accgaacccg ctacgggcgg
    15181 tttggagcct gaaacagacg aaaccggcac cgccacggac gggacagaaa ccgaggccga
    15241 aaaagatacc ggccctctga ccggcaagga catcaccgac ctgttttccg cgctgttcgg
    15301 ttccaaggtg agcattgccg ccacggacga cggcatccag atcaccaccg gcaccgacaa
    15361 aaaagaacct gaacagaccg gcaccgtcac caccaacggc ggcaggctga atgtccgcac
    15421 cggcgcgggg cttgacaaga ccgcgttcac ccagcttccc aatgggacga cggttgaggt
    15481 agtcggcaca gacggcgact ggattaaaat tctcttgccg gagcgcatcg gctatgtcca
    15541 ttccgactat atgaccgtca gcgaaaagga agtagcggct accggcgagg gcggtttttc
    15601 cctctccatt gacccggagg aaattgcttc tctgctggaa ctgttcaatg gcggcggggc
    15661 cagcgccgcc ctgtccccgg acgggaacct gtcactgatt gacgacatcg gcagcgccgc
    15721 gaaaaatggc aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt tttatctgat
    15781 tattgaccgg gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg tggatgaagc
    15841 cgatctgctg acgctgatgg aggacgggga aaccgtcacc cccgctgctg tctgctcctg
    15901 taccacgaaa tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta aaaccaacct
    15961 gaccgagtgc agcggcccgg agccgcagga accccagccg gaggaacccg aagccccgga
    16021 ggaagaaccc aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg cgctggcggg
    16081 cggcggtgcg gccctttact atttcaagtt caaaaagccc aaggctgaaa ccaccggcag
    16141 cgacgatttg ggcgagtatg attttgggga ggacgaggat ttggaggacg aggaacccga
    16201 aaccgagttt gacccggaag ctgattttgt gagtgaacag gaggacgagg aatgacatat
    16261 aaacttgtaa tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt gatcggggcc
    16321 acggagaaac acgacggcta tttggagggg aacggctatc tggtgagctg gtgcatcgga
    16381 catctggtgt cctttgccga cgccggacgc tatgacgagc gtttcaaaaa atggcgctat
    16441 gaggatttgc ccattctccc ggaaacatgg cagtacatcg tccccgacga caagaaacag
    16501 cagtttgacg ttctctgttc gctgatgcag cgccccgacg tgaccggcct tgtgtgcgcc
    16561 accgacgccg ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat ggcgggatgc
    16621 caaaagccct ttgagcgcct ttggatttcc tctatggagg acagtgctat taaggacggc
    16681 tttgcccacc tgaaaccggg cggcgactat gatccgctgt atcaatcggc cctgtgccgg
    16741 gccaaggccg actggctgat cggcattaac gccacccggc ttttttccgt cctgtaccac
    16801 aagaacctga ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt ggacagggac
    16861 gccaagatta ccgatttcaa gaaagagaaa tatcacatcg tccatatcac ggcgggcggc
    16921 gcggacgcgg taagcagccg cttttccgac gctgccgagg caaacaccgt caaggcggct
    16981 tgtgcaaaag cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac ggaacagccg
    17041 cccaagctct atgaccttac caccttgcag cgggaggcta accggctttt cggctatacc
    17101 gccaagcaga cccttgacta tgcccaggca ctgtatctaa agaagctgct gacctatccg
    17161 agaacggata gtcaatatct taccgacgat atgctgcccg ccgtggaaag ccttgtgtcc
    17221 ggcctgtggg agctggtgcc gtttgccaag ggcctgaacc tttccccgca gtttgaccgc
    17281 atcctgaaca gcaaaaaggt gtccgaccac cacgccatta tccccaccgc tgaatttgtg
    17341 aagcagggct ttggcactct ggccgagagt gaaaaaaagc tgctgtccct gatctgctgc
    17401 aagctcttat gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc cacatttacc
    17461 tgtgccgggc aggagtttac cgccaagggc aagaccgtcc tttcccccgg ctggaaagag
    17521 attgaacgcc ggttccgttc ttccttaaag accgacgccg acgaggacgc cgaggcggtg
    17581 cgggagctgc cggagctttg cgagggccag acctttgcag acgtggccgc ctctgtcacc
    17641 gagcatttca ccacgccgcc caagccctac accgaagata cgcttttgtc cgctatggaa
    17701 cgggccgggg ccgaggatat gccggaggat gccgagcgca agggattagg cacaccggcc
    17761 acccgcgccg ccattctgga aaagctggtg cagatgggct ttgtccagag aaaaggcaag
    17821 cagctcattc ccaccaagga cggcatcaat ctggctgtgg tgctgccgga ggcgcttact
    17881 tctccccagc ttactgccga atgggaaaac cggctgaccg agatcgccaa ggggcaggcc
    17941 gatcctgacg aatttatgga cggcatcgag acgcaggcgc gggagctggt gcagacctat
    18001 tcctgtatca gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc tatcgggacg
    18061 tgtccccgct gtggtgaggc tgtctatgag ggcaagaaaa actactactg cggaaaccgg
    18121 gcctgccagt tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa gaaagcgttc
    18181 accccgaaga tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa gggcctctat
    18241 tccgtaaaga ccggcaaaac ctacgacggg acggtgcttt tggctgatac cgggggcaag
    18301 tacgtcaact accgcattga acacaggagc taaaggacag taacacagca agcataggga
    18361 gtgtggctac actcccggta aaatcccccg gccgcgtccc tgccgggccg gggatttttg
    18421 cttgttgggg aagtccccaa ccccctattt tttgaaacag aacagagaaa ggagtgaccg
    18481 cgtgaacgaa gtattttcta tccaagtcca taaccggcag tttttggaac agggcaaaca
    18541 aggcgtctgg ctggatttgc ccaccacaac cgaaacgctg caagccgccc tgcgggatgt
    18601 tggcattact gccgacaatc cgcaggattt ttttatcaac gggtattcct gcccggagga
    18661 cagacacctt gccattccct atgacatggt gctggccgcc gacgtggacg agctgaattt
    18721 ccttgccgcc cggctgggcc agcttgacgc cgccgagatc gcagagctga acgccgccct
    18781 gcaaaatccc cgtggcggct ttgagaatat cggccagatc atcgactaca ccgaaaatgt
    18841 ggattactat gtgcatcttc ccgacgttag gggggatgga cagttgggcg actactacct
    18901 gaaccgttcc ggcatggtgg atatgccgga ggaatggaaa gccgggattg atacggcgca
    18961 gtttggacgc cacattgccc agcaggaaca aggcgttttt acccagtacg gctatctggt
    19021 gagaagcggc gacgaatggc agcgcgtcca tgagggccag ccggtgccgg aggaataccg
    19081 ggtgatgcac tacccccagc cggaagctga ccgggaggaa ccgcaggccc agcagccgga
    19141 ggcatccgaa agccccgaca aagcgccgcc cccggttatc ccgatcatgc tggacggcat
    19201 gaacagcaaa gaacgcatga aagtgattac tgaccgtctg gaaaccggcg tacaggagct
    19261 ttttgaaagt agccgctata tggcttactt atccgctatg tccaagatgc acgattacag
    19321 tctgaacaat acgctgctga tcgccatgca gggcggttcg attgtggcgg gctataacag
    19381 gtggcaggat gaatttgacc gccatgtgaa gcggggcgag aaaggcatta agatactggc
    19441 cccggccccg ttcaaggtta agcagcagat ggagaaaacc gacccggcca cccagcagcc
    19501 cgtgatcggc gcggacggca agcccgtgac cgaagaacag gaagtgacgg ttccggcctt
    19561 taaggtggtg acagtctttg acatcagcca gaccgagggc aaggattttc ccatgcccta
    19621 tatcagtgaa cttaccggcg acgtgcagca gtatcaggat ttctttgccg cgctggaacg
    19681 gacatcaccc tatcccataa gcattgagcc gatttcagac ggggccaagg gccgctgctt
    19741 ctacgcaaaa cagcgcattg ccatcaacga gggcatgagc gaattgcaga ccctaaagac
    19801 cgccatccat gagattgccc atgccaagct ccacgccatt gaccgggagg cccccgccga
    19861 ggaacaggcg gcccggccag accgcagcac ccgcgaggta caggccgaaa gcgtggccta
    19921 taccgtctgc cagtattacg ggctggacac gtcggactat tcttttggct atgtggccga
    19981 ttggagcagc ggcagggagt tatccgagct gaaagcctcc cttgaaacca tccgggccac
    20041 ggccagcgaa ttgataaccg agatcgacgg ccattttgcc cagcttcagc aggagcgcga
    20101 ggcccggcag gaacaggagg ccgtcacggt tgacgttacc ccggaggcag cggccccggc
    20161 gcaggccgag gaagaacagg ccccggccct tgatccggca gccgagccgc ttgtgactgt
    20221 gctatggagc gaaagccccc agctaaagga cggccagcaa atgccgttgc atgaggccga
    20281 cgcgctgttt gcccgtctgg atgcagcata tccggcagag gcaggctatg acaaaaccgc
    20341 gttccgcatt gactgtatct ttcagggcga gccggacagc tacgaggggc ggcaggattt
    20401 tggagatagg gacggttccc tgatcgaaca tatccagagc taccaccaat tttacgcgca
    20461 ggacgaaaac tggaaaaagc atgtaatcgc tacaaccggc atagagggct gggagcagga
    20521 caaggcccat cgggatatgc tgttaggcga gtttgtcccc tatatgaagc tgcattgcag
    20581 ccttgcccaa atggaacaga cggcgcaggg ccgcttacag tccggggaaa ccctgacgcc
    20641 ggaggaaacg gcctatttgg gggcgcttct ctcttatgtg caggagtgcc gccccatgct
    20701 caatcagggc cagtatcagt tgccggagcc gccgaaactg gctgacttcg accagagctt
    20761 gcaggactac aaagcacagg tacaggccga gatcgcgcag gaggccgccg cagccggtat
    20821 gaccgtggag gaatacgcgg cggctggcta tgaagcaccc caacaggaca ccttttccat
    20881 ctaccagctt cgaggcgggg aggaaacccg cgactatcgg tttgagccat acgagcgttt
    20941 acaggccgca gggctggccg tggacagtgc caactatgac cttgtttata cggcaccgct
    21001 ggataaaagc acgtctttgg aggatattta ccgcacattc aacatcgacc acccggcaga
    21061 ttttagggga cacagccttt ccgtttccga cgtggtggtg ctgcaccaga atggacagga
    21121 caccgcccac tatgtagaca gcttcggcta tatggaagtg ccggagtttt tgcgggaaaa
    21181 tcccctgcgt accgccgaac tgtctacgga gcagaacgaa aacatgattg acggcattat
    21241 caacaatacg ccgcccgaac cgcagccgga ggaacccgaa cagctatctt ttgaggatgt
    21301 ggcagttgct tccggccaga cacaggagga acccccggag gctgcgcccc cgcaaacggc
    21361 tgtccgctat tatcccatca atgagggagc cgcccgccgc gccaaggaag ccatgagctt
    21421 ttctgactac cggcccggca gcgccacggc ggaataccgg cactatgtcg atcaggccgt
    21481 ggaaattgcg gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa agatagacag
    21541 cctccttgac acctacgccc gcaagctggc cgagaacatg aataagggct atgagattac
    21601 cgcccgcgtc ccctctgtcc tgattgccgg gccgtccaat ttcccggtac gggcgaaaga
    21661 aaagcagaac gccgccagtg accgcaacat ggaggaattt cagtacattc agggcttgct
    21721 tgacaaaatc cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc aggccgtttc
    21781 caagctggaa aagaaactgg aaaagctgga agcgtcgcag gagttgatga aggccgtcaa
    21841 tgcctactac cgcaagcatg ggacgctgga cggatgcccc catctgatcg aaagaggcat
    21901 tgaaaacctg aaagcggata tggcgtcggg ctggcactat gagaaaaaac cgttccagtc
    21961 ctggcagctc tccaacaata acgccgagat acgccggtta aagggccgca ttgaggaatt
    22021 gacccgccaa aaggaggcgg cctatgtggg atgggagttt gacggcggca ctgtggagat
    22081 caaccgggtg gccaaccgcc tgcaaatctt ctttgagggg aagccggatg ctgctgtacg
    22141 cgacgagctg aaaagcaacg gtttccgctg gtcgcccaag gccggggcat ggcagcggca
    22201 gttaaacgac accacaatcc gcgtcgctga ccgtatcaag tgcatccagc ccctttccgg
    22261 cgaaaagccc agcgccttac agcttgcggc ccgcagggag aaagaaaagc cctctatccg
    22321 ggcgcagctc aacgcggcca agaccgaaca ggccaagcag ccaccggcgc gggaaaaatc
    22381 aaagggattg gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc tggccgcgct
    22441 ggaacagatc atgcggcaga acaccgcgtt ttatcaaagt gactttggca ttgacaaaga
    22501 cattatcagg cgggatgccg ccagcgatca ggccgcagac aagaccctgc tctggatgtc
    22561 ccggccctcc gggacgtact gtttccggga acgtgatgtt ttcctgaaag acacccgaca
    22621 gtacaacaca tggaaatttt acggggagca gacccgtgac aagatactgg cctacgcggt
    22681 ggagctgacc ggcacacagg gcggcactat ccggggcaat ctctatgagc tggactatcc
    22741 gcagcatttc cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc ggctctttta
    22801 tgagcatggc acccgtgaca tcccggagag ccagcgtttt gacggaagcc ctgaccgggt
    22861 attgggaaac tttttgtact atgaggccca gccccatgac ccggctgtgc tgcaggaggc
    22921 gctacggacg gagcagcaca gccgggggcg ggccgtcccc ggcgatttca aagtccacgt
    22981 caccgctttg catgacagcc ggattgaaac cgaggcccgg cgtattgtgg acagcctgaa
    23041 agatctgtcc gcgcctaaca gccccaataa aacgcatttc atggtggaga tctccccgca
    23101 ctttgcggcg attgcttcca ccaaggataa tgaccggctc tttgccatgc tgccctataa
    23161 atccctgtgt tttaccggga tgaaaggcag gcatggcgtg tatgccgtga ttaaccggga
    23221 cgagcgccgg gatgtgagta ttcgcaggcc ccggccctct atccgcaagc agctatccga
    23281 cagcaaacag gccgccagcc caaaaaaggc ggcggccaga accaaaaaga atgaattgga
    23341 ggtataagga atgatgcact ttaccgtgga ggaagaaaac ctgatctgta tgtaccacaa
    23401 tgcagaccgg cgcagaacga taaacaggct caccgccgtc atgccggtta tggatgggga
    23461 tatgcgggcg caggcccagc agaccctttc caagctggaa cgcatgacgg atgccgacta
    23521 tgacggccag aagttccact tcacagacga ataacgaaca gcagacgccg ggcgcggcgg
    23581 ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc atttagaacc
    23641 cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc gggagaagcc
    23701 ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat tagacccctg
    23761 aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt gatagccgca
    23821 aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg ggaagctcgg
    23881 caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa tgactttctt
    23941 ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt agtcagcata
    24001 atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt tgatcgtttc
    24061 atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact gattttttct
    24121 atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc tggtaaattc
    24181 tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata ccccgctgac
    24241 catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt aggtttccag
    24301 tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga gcaggccgcg
    24361 cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca gcagcgtttc
    24421 acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg gaggacagcg
    24481 gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat acattcttcg
    24541 ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg cgtcagcttc
    24601 cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct tgcgtccatt
    24661 cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg ccgtattcct
    24721 gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc tcggccaatc
    24781 gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc tgtcccttta
    24841 gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc cggaattttg
    24901 ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg taggtagaaa
    24961 tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg agggtagcgg
    25021 cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc gggtaggtgt
    25081 ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa ttgtggagct
    25141 tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc agatcaatcc
    25201 gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct ttgatacgct
    25261 ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta aagcgttcct
    25321 gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc tgcgcctgca
    25381 tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca atggctgctt
    25441 tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca tagctcttgc
    25501 ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc ctgtcgctgg
    25561 tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga tttaccatat
    25621 ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg gtagtcagca
    25681 catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc tcatgggcct
    25741 gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc agattattgc
    25801 ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt tcataagagc
    25861 aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg atgtagtcaa
    25921 gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata tctgcgccag
    25981 cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt tcagcctctt
    26041 tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg cccggatgtc
    26101 gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt aggctgaaaa
    26161 gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct ccggcgtcac
    26221 agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc ctttccattc
    26281 aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg acggggcagg
    26341 ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt ctccactaac
    26401 aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga aaaaagtgca
    26461 aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga tgccagttac
    26521 acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc aatcgcgccc
    26581 tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac cctttgccgc
    26641 agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg aagcccgcag
    26701 gctttaataa gctgtatgac aaccggcaga ctgggaatgg cccgtcgttt tcgatgttgg
    26761 caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg
    26821 cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat
    26881 tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg
    26941 tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa
    27001 agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg
    27061 cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc
    27121 cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga
    27181 caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg
    27241 gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat
    27301 agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat
    27361 ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa
    27421 acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca
    27481 tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt
    27541 gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg
    27601 ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat
    27661 atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc
    27721 tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa
    27781 gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg
    27841 tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta
    27901 aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat
    27961 cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa
    28021 accaataata tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta
    28081 aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc
    28141 ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat
    28201 ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga
    28261 tggaaatgag gcatacacca agttttacga aaacacttat caactggtta ttcttgatat
    28321 tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac
    28381 tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc
    28441 agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga
    28501 agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact
    28561 tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga
    28621 atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat
    28681 tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc acactcatat
    28741 caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata
    28801 ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat
    28861 acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt
    28921 gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg
    28981 gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac
    29041 aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca
    29101 ccaaatgcca atacatcaaa tagttttaga cccaactttc tttatgtggt acatagagat
    29161 gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg
    29221 acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat
    29281 atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg
    29341 gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct
    29401 catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa
    29461 atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat
    29521 gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc
    29581 ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag
    29641 ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc
    29701 atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc
    29761 ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg
    29821 tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac
    29881 acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt
    29941 tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca
    30001 ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcagaaa aagtgggcga
    30061 agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg
    30121 ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac atcaacattg
    30181 taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga tagagtgaga
    30241 taagaacatc taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat
    30301 cagattattt agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc
    30361 gactcgtttc aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg
    30421 caattcgagg gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg
    30481 cagttctttt ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc
    30541 tttgtcgtga aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc
    30601 agagatcgaa agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt
    30661 gcgccatttg gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga
    30721 caacgccttt aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt
    30781 catgattcgt acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg
    30841 atccacaacg agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt
    30901 catgtccgtc acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata
    30961 agaaacgaga gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc
    31021 atccatacaa gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat
    31081 taataaacaa cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag
    31141 tgtccgagaa acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg
    31201 tttttcagtt agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct
    31261 atcgacaatg taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg
    31321 tttcgctaaa gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt
    31381 tttctttccg ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg
    31441 acgaatttct ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt
    31501 cgattccaca tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg
    31561 atgaagatca ggcatacaga acgctccttt gattgtgttg tggtgattca attctaacgg
    31621 agcttctgta tgttttgttt tttcatgctc aaaagggacc gatagaatga ttttctatca
    31681 gtcctagaaa ttatagagcc gattttgacc gacaggtata accctgccgg tctttttttg
    31741 ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt gccataataa
    31801 caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat caaatggaaa
    31861 aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa tccgtggaaa
    31921 agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg tttttggggt
    31981 ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca actcatacag
    32041 gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc gccaaagaac
    32101 agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc atccccgcac
    32161 agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt cggatttctc
    32221 cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg attgtcgcat
    32281 ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt gccgaatata
    32341 aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg gtggccgtgc
    32401 cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac ggaattcatt
    32461 caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt ggttttattc
    32521 gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg tggcattacc
    32581 gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc cttgaggaat
    32641 atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa gattgacaca
    32701 gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct tttatgcggg
    32761 aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc tttcccattt
    32821 gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca tgatatacca
    32881 ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct tattgataag
    32941 accgggggaa accttttctt tctggttgtt cctgcatacg ccattgacaa ttatccagcg
    33001 cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta aaggggtgga
    33061 tgcaaccatt tcagagggct ggattgattt aaaagtgcga aacgataccg actgcaccta
    33121 ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgttcg ccgacaaaga
    33181 gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg aaagtggcgg
    33241 gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg gggaaataat
    33301 agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc cggaaagtgt
    33361 ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt tggatgcgaa
    33421 caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat ccctacgctg
    33481 attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca atgcgttagc
    33541 gtaagccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa ggtcggggta
    33601 aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc cgccgagaga
    33661 atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga ttatgctttg
    33721 atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt ggcgcaacaa
    33781 gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt gggagttatt
    33841 ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg atgccatgtg
    33901 ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga tgagcttcta
    33961 aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg ccatctgatc
    34021 ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac cgggcgcgga
    34081 gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct gggtggtgcg
    34141 gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc gcagaaagtg
    34201 cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat cacaccccat
    34261 acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat caggaattgt
    34321 ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca tcttcggcgg
    34381 ttgctcggag gaacatgatg tgtcggtaaa agccgcaata gaaattgctg cgaacattga
    34441 tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat ggaagctatg
    34501 caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct ccccggatag
    34561 gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc gtattgatgt
    34621 ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt gcgatacagg ggctgtttgt
    34681 attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt gcatggacaa
    34741 atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat ttcaaatgat
    34801 tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg tgaagccggc
    34861 acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac ttaacgctgc
    34921 gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga tttcgggctg
    34981 tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg aagtggatca
    35041 aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg aaaaaggctc
    35101 agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa atcgggtgca
    35161 ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc gtgttgatct
    35221 ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccctgc ccggttttac
    35281 atcgtacagc cgctacccac gtatgatggc cgccgcagga atcacgcttc ctgcactgat
    35341 tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg ttttttgttt
    35401 ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg ggataacttc
    35461 acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa ggccgtggcg
    35521 tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt gcttttatgg
    35581 gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc gcaaccggag
    35641 gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt gattacaaag
    35701 ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct tacgctctac
    35761 cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat ggacgaacgg
    35821 tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag atgcttgcgt
    35881 aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca ctataagttg
    35941 attgatgagc cataccccga tacctatttt aattttgccg tttcataatg aaagtattgg
    36001 ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat cttttactgg
    36061 aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat ctgcccagcg
    36121 gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt gtattttgct
    36181 ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag gtgtggcggc
    36241 ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca cttccaccac
    36301 tcaaaaaaag ccttgcccct c
//
LOCUS       DAYYCM010000017        34673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000017 DAYYCM010000000
VERSION     DAYYCM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.018"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHAY1_001023"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHAY1_001023"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8593035.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHAY1_001024"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHAY1_001024"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8593036.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHAY1_001025"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHAY1_001025"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8593037.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHAY1_001026"
     CDS             2179..2499
                     /locus_tag="ACHAY1_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8593038.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHAY1_001027"
     CDS             complement(2611..3468)
                     /locus_tag="ACHAY1_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8593039.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHAY1_001028"
     CDS             complement(3576..4880)
                     /locus_tag="ACHAY1_001028"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8593040.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHAY1_001029"
     CDS             complement(4877..5386)
                     /locus_tag="ACHAY1_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593041.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHAY1_001030"
     CDS             complement(5561..7696)
                     /locus_tag="ACHAY1_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8593042.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHAY1_001031"
     CDS             complement(7779..9434)
                     /locus_tag="ACHAY1_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8593043.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHAY1_001032"
     CDS             complement(9436..9750)
                     /locus_tag="ACHAY1_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8593044.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHAY1_001033"
     CDS             9920..10180
                     /locus_tag="ACHAY1_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8593045.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHAY1_001034"
     CDS             10194..10508
                     /locus_tag="ACHAY1_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8593046.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHAY1_001035"
     CDS             complement(11238..11792)
                     /locus_tag="ACHAY1_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8593047.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHAY1_001036"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHAY1_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHAY1_001037"
     CDS             complement(14153..14869)
                     /locus_tag="ACHAY1_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8593048.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHAY1_001038"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHAY1_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8593049.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHAY1_001039"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHAY1_001039"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8593050.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHAY1_001040"
     CDS             complement(18339..19799)
                     /locus_tag="ACHAY1_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593051.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHAY1_001041"
     CDS             complement(19789..21084)
                     /locus_tag="ACHAY1_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8593052.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHAY1_001042"
     CDS             complement(21416..22093)
                     /locus_tag="ACHAY1_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8593053.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHAY1_001043"
     CDS             complement(22123..23214)
                     /locus_tag="ACHAY1_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8593054.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHAY1_001044"
     CDS             complement(23315..23749)
                     /locus_tag="ACHAY1_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8593055.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHAY1_001045"
     CDS             complement(24333..25790)
                     /locus_tag="ACHAY1_001045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8593056.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHAY1_001046"
     CDS             complement(25787..27682)
                     /locus_tag="ACHAY1_001046"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8593057.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHAY1_001047"
     CDS             complement(27740..29515)
                     /locus_tag="ACHAY1_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8593058.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHAY1_001048"
     CDS             complement(29565..31061)
                     /locus_tag="ACHAY1_001048"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8593059.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHAY1_001049"
     CDS             complement(31255..32877)
                     /locus_tag="ACHAY1_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8593060.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHAY1_001050"
     CDS             complement(33205..34518)
                     /locus_tag="ACHAY1_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8593061.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         9854 a         7494 c         5848 g        11477 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atc
//
LOCUS       DAYYCM010000018        34663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000018 DAYYCM010000000
VERSION     DAYYCM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.019"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHAY1_001051"
     CDS             591..1280
                     /locus_tag="ACHAY1_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8593062.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHAY1_001052"
     CDS             1267..2475
                     /locus_tag="ACHAY1_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8593063.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHAY1_001053"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHAY1_001053"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8593064.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAY1_001054"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAY1_001054"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8593065.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHAY1_001055"
     CDS             complement(6578..7273)
                     /locus_tag="ACHAY1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8593066.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAY1_001056"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAY1_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8593067.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHAY1_001057"
     CDS             complement(8472..8663)
                     /locus_tag="ACHAY1_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8593068.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHAY1_001058"
     CDS             complement(8684..9574)
                     /locus_tag="ACHAY1_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8593069.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHAY1_001059"
     CDS             complement(9561..10328)
                     /locus_tag="ACHAY1_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8593070.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAY1_001060"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAY1_001060"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8593071.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAY1_001061"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAY1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8593072.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAY1_001062"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAY1_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8593073.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHAY1_001063"
     CDS             14899..15282
                     /locus_tag="ACHAY1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593074.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAY1_001064"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAY1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8593075.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHAY1_001065"
     CDS             complement(16226..17044)
                     /locus_tag="ACHAY1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8593076.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAY1_001066"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAY1_001066"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8593077.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAY1_001067"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAY1_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8593078.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAY1_001068"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAY1_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8593079.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHAY1_001069"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHAY1_001069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8593080.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHAY1_001070"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHAY1_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8593081.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHAY1_001071"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHAY1_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8593082.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHAY1_001072"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHAY1_001072"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8593083.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHAY1_001073"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHAY1_001073"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8593084.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHAY1_001074"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHAY1_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8593085.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHAY1_001075"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHAY1_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8593086.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHAY1_001076"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHAY1_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8593087.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHAY1_001077"
     CDS             28352..30325
                     /locus_tag="ACHAY1_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8593088.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHAY1_001078"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHAY1_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8593089.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHAY1_001079"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHAY1_001079"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8593090.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHAY1_001080"
     CDS             33204..34496
                     /locus_tag="ACHAY1_001080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8593091.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10565 a         6735 c         6854 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DAYYCM010000019        33266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000019 DAYYCM010000000
VERSION     DAYYCM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.020"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHAY1_001081"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHAY1_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8593092.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHAY1_001082"
     CDS             500..862
                     /locus_tag="ACHAY1_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8593093.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHAY1_001083"
     CDS             914..2596
                     /locus_tag="ACHAY1_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8593094.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHAY1_001084"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHAY1_001084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8593095.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHAY1_001085"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHAY1_001085"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8593096.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHAY1_001086"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHAY1_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8593097.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ACHAY1_001087"
     CDS             6431..7435
                     /locus_tag="ACHAY1_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593098.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ACHAY1_001088"
     CDS             7436..8380
                     /locus_tag="ACHAY1_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593099.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHAY1_001089"
     CDS             8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHAY1_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8593100.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ACHAY1_001090"
     CDS             9369..10283
                     /locus_tag="ACHAY1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593101.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ACHAY1_001091"
     CDS             10309..12090
                     /locus_tag="ACHAY1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8593102.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /gene="rnc"
                     /locus_tag="ACHAY1_001092"
     CDS             12438..13124
                     /gene="rnc"
                     /locus_tag="ACHAY1_001092"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8593103.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ACHAY1_001093"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ACHAY1_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8593104.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ACHAY1_001094"
     CDS             16734..17543
                     /locus_tag="ACHAY1_001094"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8593105.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHAY1_001095"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHAY1_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8593106.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHAY1_001096"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHAY1_001096"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8593107.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ACHAY1_001097"
     CDS             complement(21095..21547)
                     /locus_tag="ACHAY1_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8593108.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ACHAY1_001098"
     CDS             21697..22647
                     /locus_tag="ACHAY1_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8593109.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ACHAY1_001099"
     CDS             22640..23599
                     /locus_tag="ACHAY1_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8593110.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ACHAY1_001100"
     CDS             23596..24351
                     /locus_tag="ACHAY1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593111.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ACHAY1_001101"
     CDS             24374..25327
                     /locus_tag="ACHAY1_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8593112.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ACHAY1_001102"
     CDS             complement(25971..26267)
                     /locus_tag="ACHAY1_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8593113.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ACHAY1_001103"
     CDS             26622..26978
                     /locus_tag="ACHAY1_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8593114.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ACHAY1_001104"
     CDS             26988..27887
                     /locus_tag="ACHAY1_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8593115.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28407..29135
                     /locus_tag="ACHAY1_001105"
     CDS             28407..29135
                     /locus_tag="ACHAY1_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8593116.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ACHAY1_001106"
     CDS             29280..31307
                     /locus_tag="ACHAY1_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8593117.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="ACHAY1_001107"
     CDS             31415..32512
                     /locus_tag="ACHAY1_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8593118.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33266
                     /locus_tag="ACHAY1_001108"
     CDS             32819..>33266
                     /locus_tag="ACHAY1_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593119.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11139 a         5762 c         7125 g         9240 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa agttga
//
LOCUS       DAYYCM010000020        33140 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000020 DAYYCM010000000
VERSION     DAYYCM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.021"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1155
                     /locus_tag="ACHAY1_001109"
                     /pseudo
     CDS             1..1155
                     /locus_tag="ACHAY1_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
     gene            1267..2718
                     /locus_tag="ACHAY1_001110"
     CDS             1267..2718
                     /locus_tag="ACHAY1_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8593120.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3067..3699
                     /locus_tag="ACHAY1_001111"
     CDS             3067..3699
                     /locus_tag="ACHAY1_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8593121.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4287..5726
                     /locus_tag="ACHAY1_001112"
     CDS             4287..5726
                     /locus_tag="ACHAY1_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8593122.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6021..6239
                     /locus_tag="ACHAY1_001113"
                     /pseudo
     CDS             6021..6239
                     /locus_tag="ACHAY1_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6488..6703
                     /locus_tag="ACHAY1_001114"
     CDS             6488..6703
                     /locus_tag="ACHAY1_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593123.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6920..7176)
                     /locus_tag="ACHAY1_001115"
                     /pseudo
     CDS             complement(6920..7176)
                     /locus_tag="ACHAY1_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7363..8274)
                     /locus_tag="ACHAY1_001116"
     CDS             complement(7363..8274)
                     /locus_tag="ACHAY1_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8593124.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8293..9297)
                     /locus_tag="ACHAY1_001117"
     CDS             complement(8293..9297)
                     /locus_tag="ACHAY1_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8593125.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9294..11417)
                     /locus_tag="ACHAY1_001118"
     CDS             complement(9294..11417)
                     /locus_tag="ACHAY1_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8593126.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11422..13869)
                     /locus_tag="ACHAY1_001119"
     CDS             complement(11422..13869)
                     /locus_tag="ACHAY1_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8593127.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13856..14245)
                     /locus_tag="ACHAY1_001120"
     CDS             complement(13856..14245)
                     /locus_tag="ACHAY1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8593128.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14305..14808)
                     /locus_tag="ACHAY1_001121"
     CDS             complement(14305..14808)
                     /locus_tag="ACHAY1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8593129.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14821..15045)
                     /locus_tag="ACHAY1_001122"
     CDS             complement(14821..15045)
                     /locus_tag="ACHAY1_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593130.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15149..17059)
                     /gene="ltrA"
                     /locus_tag="ACHAY1_001123"
     CDS             complement(15149..17059)
                     /gene="ltrA"
                     /locus_tag="ACHAY1_001123"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8593131.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17805..17942)
                     /locus_tag="ACHAY1_001124"
     CDS             complement(17805..17942)
                     /locus_tag="ACHAY1_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8593132.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17939..19123)
                     /gene="mobT"
                     /locus_tag="ACHAY1_001125"
     CDS             complement(17939..19123)
                     /gene="mobT"
                     /locus_tag="ACHAY1_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8593133.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19382..19672)
                     /locus_tag="ACHAY1_001126"
     CDS             complement(19382..19672)
                     /locus_tag="ACHAY1_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593134.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19662..20804)
                     /gene="dcm"
                     /locus_tag="ACHAY1_001127"
                     /pseudo
     CDS             complement(19662..20804)
                     /gene="dcm"
                     /locus_tag="ACHAY1_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20880..21044)
                     /locus_tag="ACHAY1_001128"
     CDS             complement(20880..21044)
                     /locus_tag="ACHAY1_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593135.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(21037..21711)
                     /locus_tag="ACHAY1_001129"
     CDS             complement(21037..21711)
                     /locus_tag="ACHAY1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8593136.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21789..23129)
                     /locus_tag="ACHAY1_001130"
     CDS             complement(21789..23129)
                     /locus_tag="ACHAY1_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8593137.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23212..23547)
                     /locus_tag="ACHAY1_001131"
     CDS             complement(23212..23547)
                     /locus_tag="ACHAY1_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8593138.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23557..23931)
                     /locus_tag="ACHAY1_001132"
     CDS             complement(23557..23931)
                     /locus_tag="ACHAY1_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8593139.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23944..24258)
                     /locus_tag="ACHAY1_001133"
     CDS             complement(23944..24258)
                     /locus_tag="ACHAY1_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8593140.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24371..27598)
                     /gene="fss3"
                     /locus_tag="ACHAY1_001134"
     CDS             complement(24371..27598)
                     /gene="fss3"
                     /locus_tag="ACHAY1_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8593141.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            28455..28709
                     /locus_tag="ACHAY1_001135"
     CDS             28455..28709
                     /locus_tag="ACHAY1_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593142.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            28873..29790
                     /locus_tag="ACHAY1_001136"
     CDS             28873..29790
                     /locus_tag="ACHAY1_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8593143.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            29783..30565
                     /locus_tag="ACHAY1_001137"
     CDS             29783..30565
                     /locus_tag="ACHAY1_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8593144.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            30566..31246
                     /locus_tag="ACHAY1_001138"
     CDS             30566..31246
                     /locus_tag="ACHAY1_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8593145.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            31239..32135
                     /locus_tag="ACHAY1_001139"
     CDS             31239..32135
                     /locus_tag="ACHAY1_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8593146.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            32347..>33140
                     /locus_tag="ACHAY1_001140"
     CDS             32347..>33140
                     /locus_tag="ACHAY1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8593147.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKM"
BASE COUNT         9947 a         6579 c         5949 g        10665 t
ORIGIN      
        1 agaatgacac taaattctga gataaaaaag ttatttctag agacagaagt tgacaaaatt
       61 gatattcaaa ataaaaagat tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa
      121 tatgacaaag ttatattagc tacaggttca aagcaaatta tgctagatat accggggaat
      181 aatttagcta atattttttc gataaagaat tttccaaatg caaaagagat ttttaaagct
      241 ttggatgata gtactattaa aaaagtagga attattggag caggatatat tggagttgaa
      301 attgctgaag ccataagaaa aagagggaaa gaagtatatc ttttcgatgt tgcagataga
      361 gtgttgtcga cttattacga ccgtagtttt tcagataaag ttgaagaaat actatcaaaa
      421 aatggaatac atctttgtct atcagaaagt cctaaaaaat atttaggaag aaaaaagatt
      481 gaaaaaatag tcactacaag tggtacccaa tactctatgg atatggtcgt acaagctata
      541 ggatttctat ctaatagtcc aattggggaa aaggaactct tgagagatgt tagtggagca
      601 tatctaacta atgaatacca gcaaacaaat ataaaagata tatatgctat tggggattgt
      661 gctacaactt tttttacgtc cattaataga agtagtgttg atttttctat ttctaatgct
      721 ctaagaacag cttatattgc aactcaacat attaacaatc aagattttac accttctggt
      781 tcccaattta caaatggctt ttctatattt aattacaatt tttacgcagc aggtttgaat
      841 ttaaaaacag cacgattatt aagtgagtct atagattata tagaaataga agatttcatt
      901 agacctgctt ttttgtgaaa aaatagtcgt gtaaaattaa gaattagctt tgaaaaaaat
      961 acaaagagaa tagtaggtgt acaactttgt agtcaagaag atttatctgg actattatca
     1021 atgttttctc ttgctataga agaacaaatt acattagaca aattgaaaat gttagattat
     1081 ttatttcatc ctagttttag ccaaccctat aattttattt taaaggcagt aacaggatta
     1141 aacattgaaa cataatcgtt actaaatgaa atcaagaaat atactgtatt actcaattca
     1201 ttgaaaatat ttatcataat ttttaataga aaacactatt aaagagaaaa aagagaggta
     1261 aatcaaatga aaaaatcttt aaaacttgca tcagcagtta tattaatgtc ccaactcctt
     1321 atagcaacac ctttccatgt gttggcaagc gaaaattcaa catacaaaat tgcaaaagaa
     1381 agaaccgaga cgactgaaat gagtaattcc gatgtaacag cagagagtag tgaacttttg
     1441 acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcatca
     1501 agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt
     1561 acgacttcaa atgccgagtc ttcaacaact tcgtcaagtg aaagtagtac gatttcaagt
     1621 accgaatctt caacaacttc aagtagtaca agtgaaataa cttcttcgaa agtgacaaaa
     1681 ccaaaccata tagaatcttc aaaaaaggaa gcgccaaaaa cggaaggaca aacaaaaact
     1741 ttttcgacac cacagcaaat agaatcaaca gaatctttcc aatcacaacc aatactatct
     1801 ggtaatgaag aaattcattt tgacaaaaat gaatctgtag aaaattttat tatgaaaatt
     1861 ggtgagtctg cgagaaaaat tggtcagaaa tatgatttat atgcttctgt tatgattgca
     1921 caagcgatct tagaatctgc cagtggtcaa agccagttag ctcaggcacc gaattacaat
     1981 ttatttggga taaaaggaac ttataatgga aactttgtca ttatggtaac aaacgaagat
     2041 ttgggtaatg gaacgcttta tacgacccaa tcaaaattta gagtgtatga aaactatgag
     2101 gaaagctttg aagattatgc caaattatta actaagggaa ttagtggaaa taaagatttt
     2161 tatgcaggag cattaaaagc taatagtaaa acatatcgag aagcaactaa attccttaca
     2221 ggacggtatg caacagatac acaatactat ttgaagctaa atgaattaat taaaacctac
     2281 gatttaacca actatgataa agaagttaaa ggtccacaaa tgagtaagac tggataccaa
     2341 gtgcctattc ataattattc aataaccagt ttgtttggaa atcgtggaaa tgaatttcac
     2401 cgaggacttg atatggcagc tacacagggt gagccgattc atgcagttaa agctggtaag
     2461 gttgttaaag cagagtttca ctactcttgg ggaaattacg tggtgataca acacgaagat
     2521 ggatcaaccg cgttatatgc tcaccagcaa caatacctag taaaaactgg agataatgtt
     2581 ttgcaaggac aaataattgg ctatgtagga agtactggga atagtactgg cccacacctt
     2641 catttggaaa tttgcaaaga tagtagtctt tctcagagca tgttagttga tccgaaaacc
     2701 ttattattcg gagactaaaa aaggtaatca atctgacaaa gtaacaagaa aatggttgga
     2761 ttcttagacc tttgatcagg atattgagac tggaattgat atctcgatta cgatgggttt
     2821 gacaaataaa acaagtccat tatcgaatat cgagtgtatt attgccgtct ttatgctcat
     2881 attttgagca ggtttcacta gatgaaaaca atttatctat cttgaggatt tcgtgtacgt
     2941 atcgttcagt cttgtattgt aaattagtca taaaatcaga ctaagaaaaa ttggaaatat
     3001 ttttttctga tttattaaaa gttaattgaa attatatggt acttctttct tgggagggta
     3061 ggtgcagtga aggaaagata tggtcacctt actgttgttg aggagattac tcataatgga
     3121 tataaaaaat atcgttgcct atgtgactgt gggaatgaaa cgattgtata tcgatcaaac
     3181 ttactatcag gtagaaccaa aagttgtgga tgtggaaatt ataaaaatag gcataaatat
     3241 aaaaatttga caaataaaac ttttggtaag ttagttgcaa tcaagccaac taaactgcga
     3301 agtgaaggaa ctgttgtttg ggaatgtcaa tgtctgtgtg ggagaaaaaa gttagtatcg
     3361 gcaaagcgac ttctaaaagg tgatgtaaaa agctgtggat gtttgaaatc aaaaaagcat
     3421 cacattgaaa ataaaaaatt cggattactt actgcaaagt atcctttgga caataactgt
     3481 tcatggaaaa caaaatgggt ttgtcattgt gagtgtggca gtatgtgtga gataagttat
     3541 agtaacttat tttttggaca tactcaaagt tgtggatgtt taaaaaggca agaaacaagg
     3601 acatgtgttg aagggacagt aatagagtgt ctaacgtcaa aattaccgaa aaataataca
     3661 agtggtgtta agggagttta tcgttctaaa gagaaataga ttgcgtatat aactttaaaa
     3721 aaaaagaggt actatttagg atcgtatgat tctattaaga gtgcagctca agctagaaaa
     3781 gatgctgaaa ataaattgtt tcaacctttg attgacaaat atcgaggtaa tcattaatta
     3841 ttgtggataa taattaacag tgatatataa acatctataa tatattattc atactattga
     3901 tatttagtat aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga
     3961 aaacaaatag gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt
     4021 gcttacgtct cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta
     4081 gaaattgcta gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata
     4141 aagcatctcc aacttacatg gttacatgag gatatcaaat cgaaattact caagcttcct
     4201 atggctagag tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac
     4261 acgatcatta gaaaggaaat cctattatgt atagttatca agaagatgca aaagtccaaa
     4321 aaaatcgctg gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg
     4381 gcagtattgt caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc
     4441 aagcggaatg ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg
     4501 gtaaaattgg cgatagccgt gggaaaatca aagtatttcg gattggtacg ctgatctttg
     4561 tcttgggatc gctgttgtgt gggttcaatc attcgctagg tattttattg ttcgcacggg
     4621 tggtacaagc cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag
     4681 tctttcccat acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt gtttcattag
     4741 gctcgattgc tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca
     4801 ttttttggat caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac
     4861 caaaagatat caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg
     4921 ctacgattat cagctttttt ggagcaatct ttttggggca agaaagtggc tttttccatc
     4981 cagtccccct tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg
     5041 aaaagaaagc agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca
     5101 tgagcttgat tacagccttg ttgatttttt cttccaactt ctttgtcaat gtagtgattc
     5161 ctttttatct gcaaaatgct cgcgggttga gtcctagcta tgctgggctg ttgatgatgg
     5221 tctttccttt gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg
     5281 gcccggagat tttggtatta gtaggcttgg gcttattgtc ggtaacccag gtcatgtata
     5341 tgtttttgtc agtggactct cctttgtggc tatacattgt cgctactgcc atcatgggtg
     5401 ccggcaatgc cttgttccag tcccccaata atacgatggt tatgagctcg gtcactatcg
     5461 aaaacttggg actggcaggc tcgatgaatt cctttgcccg aaatttgggg atggtcagcg
     5521 ggatttcgct ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccagcaag
     5581 taactaacta tatcgcaaat cggccggatg tctttttata tggaatgaag attacctttt
     5641 taggcagctt tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac
     5701 aaggaggtat tcagaaaaat gtctgaaaaa accactgttg aagaattttt ggaggcctgc
     5761 gtatgggtga gtgggatttc catggatgat ggaagatgaa gtgaacttct aaaagccgaa
     5821 aaactcaagt attatcttca aaatactagt gctcaatgag atgcttgatt tgatcacaag
     5881 aagactgcga aagccgctgg caaccgcaag ctaggtatcc caccgatgga ctacgtttta
     5941 gaggctactg gttatgaata cggagccaac gctctcatct gcataagaat acatcaccca
     6001 gattatgtaa agattttcga tacttctgtc aaaaaatcat taagatagat aaatgatttc
     6061 cgtctagtca aattggttca gaatgtagac acacagatgg taagaagtca ctcacaattc
     6121 gagaatggac ttgtcctatt tgccgtatac atcataatcg aaacgtcaac gatagtatca
     6181 atattttgac caagggctat gaatacaagc attgccttag aaaacatcaa gaaccttagg
     6241 aacaacgggg atagcttggt taataagaga taccgctgtt taggcttgca agtatactct
     6301 tttatcaaga attccgtggc tttagtcatg agaggcttaa tactatgaaa attttcttta
     6361 gcatttttga tttaggttcc ttttctaatt ataataagat gaccactaca gtaacctact
     6421 gaaatatata aacagccact gtagtaccat tttcaaagta gtctatgaga ggagaatctt
     6481 cattcttatg aacgctggaa ttgcggatat gaatttaata aaaaaaaccc ttaatgattt
     6541 tacttccaat tcaatatcaa aaggtactgg tatcaattta agcacaataa aaaagctgaa
     6601 aagtggtgaa agatcagtgg aaaagctgaa tcttttagat gctataaaaa taacggaatt
     6661 tgctatgaaa aatggaaaag ctgagattga aatatggaga taattatcgt ctagtgaatt
     6721 acttgccact tagctgaatt taacgatcct tggtgcttga agggggagat tcttgggaat
     6781 agagcatact tgcaagccta tgtctttact aacaacgata tctcttatta accaagctat
     6841 ccccgtagtt ctaaggcttt tacttttatc taagcaataa tcgtattcaa aggaaatttt
     6901 tgacccttag aaagtctcat tttatcagac gtgagcttag gcattgctga attttgttaa
     6961 ttcggtttta ggttgtgctt tgagcataat atgaaagtat tctttatcac gatttcattc
     7021 atctaaggta atgtgatgtg attctgaaat tctttcaaag gcagtttttg cataagctga
     7081 cattacatca tcaatgacat gtttacaatg tttcactact aaaataaggg ggtaaccaag
     7141 aagaaaaact gaatgattat ttgtatctaa ttccatggta tatcaaccat ttcctatata
     7201 gactgattat gacatggatt aaaataaaaa ggcactatgt cttaattgtc ggttttcgag
     7261 ttaccgaatg gcattcatca cccacctata gaggatgggc gacttctgcc tgaaataagt
     7321 tacactactt ataacttcta ataaaattag attgagtcat tattatttaa catcaacgat
     7381 tttccagttt ccattttttt gtaatactaa tttgtactga aagtagctag tggtttttga
     7441 tacattatca aggtatttta tctttgtact cactagaaaa ttttttccgt tttgtagata
     7501 aactggattc tctaactccg aaaactttaa gtcaatattg attggtcgca tcgcaccatt
     7561 ttcaacataa tactccaatt ctttttcaga agctgatgga taaagtttaa aaaatgtttc
     7621 taaaaaatcg gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg
     7681 ttttgcttca tattttgcct tgtctggctt tccaatcatt gttggcaaac tagttactaa
     7741 atgattcctg ttttcatctt caaagattgt cactcgatat gcagaagaaa ttgattgcgt
     7801 cttttttccc tctgtaatat gttgctccac taaaaatgaa acgacaaatt cttccgagtg
     7861 tttttcaaca tctagaattt ttacagattg aacttcggaa ctcgtgggaa tatctgctcg
     7921 aaccatatct tggattaaag cgagaccgtt ttctgtaagg tactgttcga gattactcat
     7981 tctcttttca attgcttctt tgttattttt ccatgaaaaa tattctttcg caaaatcctc
     8041 tgtaaaactt tcaacgccag aagtattcac taccttctct tttataacca ctttttcatg
     8101 agttgtatgt tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa gactacaaga
     8161 caataaaagc caaagggcaa gcaccatttt tcgatgctta cccacggata caactcgttc
     8221 ttttttcttt tttgggctct tttccttttg attccgttca atttttatct tcattctttt
     8281 tcctcttttc tcttatttgt atcggccagc tccgataaga tgctgccgcc aatagttttc
     8341 cgttagattc gtccacccca ctggatctcc tgcatgaaac atgcgattct cccctgcgta
     8401 aatgcccaca tgggtcacat aagtaccggc atcataggtt gaatgaaaga aaactaaatc
     8461 acctggtttg gcatctttta gatcaatatg agtcatggca tcatattgat cctgagccac
     8521 ccgaggaagt ttcaggtcaa ctttggcata acaccattgg gtcaagccac tacaatcaaa
     8581 ccccgtttct ggacttgagc caccaaacac ataggccgtt ccttcatact taaatgcctc
     8641 atcaaaaatt tcttggacta ttttgtcatc aaactgagtt gtagttaggt attgcttcac
     8701 taataaacag taaaacatat tgccataatt ataccgccac ccaccatttt ccttaatcgc
     8761 gattgggttg gggtaatcga ctttttgacc acctgatttt tcctttgaaa aagattcagc
     8821 taaagcaaaa ctatgcttct tcccgttttt cccgacatga tctagatacc cgccaccata
     8881 gttataggcc tgaataatac tatcttgatc acaccctagt tctttggaac gcttaaccaa
     8941 agacgcaaaa tagagacagc cttgtttgat ggatgcctcg gtacttaatg aattaggtgg
     9001 taatccggct gactcagaac tttgcataac gtcttccatt gtgccaccag attccacttg
     9061 aataatcgcc aaaagaattg gaatatactc tgttatatcc gattctttgg catacttttc
     9121 caccattgca cggtgtttca atacttcttc tgagacacta gttccgccag gagaagtgcc
     9181 agaactacca tttccctcac tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc
     9241 cataaaagag atggtcagaa aagttaatga aataacaccc actttcttca agttcatttt
     9301 ttctcacctg accttgattt tggacgatgg ttcattcttt tttgtacttt ttgattggtc
     9361 tgctttggca gagtggacct ctgtttcact tgttggaaag aatttttctt acttgctgat
     9421 tggcttttcc cttttgaaat gatttcttga ctagttagtt ttcgtttgat ttcgggattc
     9481 agatttgctc tcacaatccg ctttggtgct tcttttttca gcatcatatc tttctgaggt
     9541 tgagctgctt tatctattgg aatattcgcc cttctttttc cctgacgatt tgttgcttca
     9601 ctcagtatct tgcgtttttc gtccattttc gcatttcttc gttgtcgttg tttttctcgc
     9661 aaatccactc gatccgcttt ttcttggaac agtccgcgct tgaattcttc tggtgctttt
     9721 ttcgactttt cgattccttt atggagattg tatttcaaat tcgttggcgt attccttacc
     9781 tgatccttta cttgtttagt cttatctttc accaaagctt gggtatccaa aactttgccg
     9841 atttttctcc cagccaaatt cattcgggat tgtttcttat tatttgaaac tggattctcc
     9901 ttagttaagt cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa
     9961 gaaccggatg gcttaaattt ggattttgat tgttcctttg ccaacttatt tccgactgct
    10021 gcaccaccac caaaagccaa agctcgtccc acatttcgat tgagttgccg caattttcga
    10081 ttcattaaca tttgcggttt tcgcatgacg cgtcttccca ttgactggct atcattactc
    10141 tggaggttga acatactcat aatgtcgccc aatttgaaat agataccggc aaaggtcaca
    10201 atttgcagaa aagcaaccat gaaaaatgga taggttgccg aaatattgaa aaacatcgtg
    10261 gagatgctaa atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg
    10321 aataaacgaa taatcgcctt ctttcctaaa ctttcataag tcggcaacat ggacaataag
    10381 aaactaattg gcagaaacat cgcaaagata atgaagagaa tttgcgagaa caacataatc
    10441 cctgttagaa gaaaaacaaa gaaggaaatg ccaatattga acaataccaa aaagaccacg
    10501 gtccctaaac ggttcatggt cttggtaata ctgagattcg cattgtcatt gtcttcaatc
    10561 tctgccttga ccgcttcttc ccgatctttg cctttgtttt catctggact cacagataga
    10621 atcttgttga cccggtcttc accaatttct tctatgttgg aatcatcaaa ttgcagtagt
    10681 aaccaaggtt gttggacttg aatcgaaaac aaactatccc gaatcaaatc cacactatct
    10741 tttccttggc tttctgaatt gggtacaacg atctcggttc caagggtaag ggccgcttca
    10801 ctaacatctg agctaaagtc attgattttt gtgatatagg taggcgcata agcaatgaac
    10861 gaacccgata gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt
    10921 tctcgtttta ataatccggt atacgccaca tacaccccca gagctaaaat catcaataat
    10981 agaaacccaa aataaaatcc ggaggtctgg agtccatttt cagtaatacc agccaatgtt
    11041 tggatattct ttccaatact ttcagccgta tccgaaatga aatctaattt gtaagcctct
    11101 tgaaccacat aacccgtggc gttcgacaaa tacaaactca ccgtccaaat gaaattagta
    11161 atggcataaa gaccatacat cacgcttttc cctaagccat caccccaatt ccaaggcaac
    11221 caatcccaag agctatccac aaagaaatct agttgatagt tgttcagtga atatttggaa
    11281 tatagatttc ctgcttcaac tgtatcatca actagccctg tcgcctcggc taccgtacca
    11341 actaaactca atagaactat cagagtgagt atgctaataa aaacgattcc cacaatccga
    11401 aaaagtttct ttttcatccc ctcactccat tctctcttga acaggtggac gggtatcaaa
    11461 ggcatggaag agttcttcaa agactggatg tacctgaacc acgcctaccc gaccatacaa
    11521 atcctgcatc aagcattggc cattttccaa atctcgtagg cgtttttgat tgctttcatc
    11581 ttctcgatcc aagccaaaga attccaaggt tttcttgatt tcatgaatat cggtactgcg
    11641 aaacgcaaac ttcaacccga tattattctt caagcgttca tccagtaaat catcactatt
    11701 ttgcgtgaca aagtaaaccc cggcattcat agcccgccca gcacgaacga gcttattcga
    11761 taatgttttc ccttgcgcta cttgaagaaa actccacgcc tcatctaagt ctacaatttt
    11821 gaagatttct cgattactat ggataaagtc caaggcaaaa gtggaaatga cgattagcat
    11881 ggaaacacta agcaattcca tggtggtata ttcttcaaaa gtggtttctg catctggtaa
    11941 aaccaaatcc gctacttgaa tgatatttaa ctgcctatct aaactaatcg actgggtaat
    12001 cgaaccatct gagaaaagca aatgggcaaa atcataatcg gtaaaggact cgatgtgatc
    12061 cgcgatattt tccgcaactg gtgagccttc ttttcgaagc tcattaatta cttgtaataa
    12121 tccccgttga ttgctttggg taaccgtccg aatggcctta cgcaacactg gaaacttctc
    12181 cccatctcga ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta gactttcaga
    12241 atccttcttt ttctccataa tcacataagg atcaagtaat ccttgatttt ttggcttact
    12301 agttaagttg agaatattga tttcatccgc aatttctggc aaggtttctt tccagccacc
    12361 ccgttcccct tttggatcga caatcacagc ttgtccacca aaaaggacgg cgtaataaac
    12421 taagagattg ttactaaagg actttccgcc accgagtgaa ccgaggaatg ccgctgctag
    12481 cgcattggta actgaatcct tgaccccttg tgaggccaat gccggtttca ggtaaacatt
    12541 gcgccccgta tctacgttat aaccaaagta aatgccttcc aactccccta acatctgagt
    12601 ggcgccaaaa ccaagtccgg cgagaaaatc agacgtaaca tactggatat aatcattcat
    12661 gtatcgctta ctagaaggta agaattcttc atgtagcccc atcatatcgc caaaaggccg
    12721 gactagtttt acattggtat catcataaaa atctagcact tcatcacacc ggcgctttag
    12781 ttcatctact gattcggcac ttactcgaac cacatagctc agtttgtaca tggattcttt
    12841 ggactgatcc aaggtagttt ccaattcatc tacggaatcc aatgcatcta agacattgga
    12901 atttgtttca ttgtcagatt gataggcatg gttgtccaag tcttttaatt cctttttctt
    12961 attgcgaacg gtagcaagtg cctttttgtt cgtcacaatc tccacattca tactagtgtc
    13021 aattgggaat gtaaattgtt gttgctggta atagaaaagt tctgaggagg gaaactccat
    13081 ttcccccaca attgtattaa ttgttaagta cgctacatat gattcatgat tctcatgttc
    13141 catgcataag taccggggct tttcttcaat caaacaacga ctgggacgta ataagtcgta
    13201 acgtttcacc aaagtttccg acttcagctt tttctttggt agctgaaatt catattcttc
    13261 aaatggtgta cccttttcgc catagatatg ttcaatcaaa tacatcagat cactcggtgt
    13321 gactcgtctg actttaaatc ttcgagccaa tttactttcc attagttttt caagcttggt
    13381 atatcggcga atttcttcat tggataagga aacaaaatcg cccatcaaat gatgattcac
    13441 tccatagaca aattcttgaa agccggagaa gaaagatttc ttgagattct ttagattgac
    13501 ctcttcatct gtcgcaatca gtttgaagcc aataaaaaag cgataatcaa tctgactatc
    13561 gccaatcatg gatactaatg cctctgtctg taaatcaatt cgttgtttcg ctacttctgt
    13621 taatcgcccg gtaatttcct tttttgatcg ttcttgcgtg gcacgaatac tactttctgt
    13681 ggcaatttgt aaggcatgaa tcttgccttc acgattttgc gcaatcaatt gacgaaagtt
    13741 atcgtggact tgatatttct gttcaggtga caaaaaggag taattgtaag gtattaactc
    13801 atagtaggca aaacattctc cctcttgatt aaagacaaga ttattttcaa tatacttaat
    13861 cgggtacata gttcacactc cttaccagag taatgggttc gtcccatcgt ttcttctgtt
    13921 ctttgacttt ctttcctgcg aaagtgacct taaatcgtaa gtgatagcta ataaaagatc
    13981 gtaaaaatcc caatggtttc ttaccgtcaa acgttttttg cgacatgaac caagtaactg
    14041 ccaccggtat acctaagtat tttaaaaagg ccccctctat catagatagt ggaggcacat
    14101 gggcaaatac gatgacaaac agcaaggata aaacaaacca agccatctgg ttaaaagtca
    14161 caggaaaagg cagttgaaag tcattaattg cataaatgac cttttcaact gcccaaatac
    14221 tggtgtagct ttttattttc ttcatcaaat cattccttta aaaagaagga tagtttagaa
    14281 atcaactacc cttcaataat tttgttaata tggatactca aaaacacctc gactcgttac
    14341 caaataattg cctccaatct ccaagtctct accacacgca tcaaaatcaa tatacgtctt
    14401 caagtgttct ggtacttctc caaaatcacc agcttctaca atcaagaaat atgctacatc
    14461 actcatatca tcgcaatctg gataatagat aatatcatcc acatgctcta ccatctcctc
    14521 aaaagaattg aagaaagcat gttgaatctc agaagcaact tcaccaactg gcgttccttc
    14581 aagttcctcc gctaagccac acaaccgatt aatttcctca attggcgtat actcatcaat
    14641 ctcaaaggga agttcataat catgaattgc atattcctcg tattcgtcat ttaacccaat
    14701 tcgttctttc acttcatcaa aatcgacagg tggggtaaac cacgccccca ccaattcacc
    14761 ttcgttatat ttcccgaggt tcgcaatata aacacgcatc tgttccatcg tgcttccccc
    14821 ttacgcaccg acaattcggt taaacaaact gagtaagaca tccttcaccc cagaagcatt
    14881 aaagaccaat ccgaccgcaa tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc
    14941 ccgcttaaat cctaaaaaca aaccaatgac cacaattgcc agtaagacta aggattgggc
    15001 attggttaaa aaccattgat atatggatag gtaagttcct gatattataa atatctcaaa
    15061 cttttccccc cattcacacg gagcgtgcga ctttcaccgc actccgcgtt ccatcgtgtt
    15121 tatattgaaa agtatttatg tagtagacct atatcaagtt taattcttga ttgccagcta
    15181 atactctata tttatttagt cttatgagct ggactttatt tagcttcaat agagctatat
    15241 atttttgtat tgtttcttct gttttagcgt gtattagacg gtgtaccgcc tcttttacca
    15301 aaataagatt tttataatcg tcatttccac cagctccagt tggtacaata tgatgacaat
    15361 gtatttcatt agcttgttga aattcttcca gagttatagc acattttcca tgttgagcag
    15421 aaaatagcga aattcgattg tctgcgtatt ctattgtgcg attttccaaa gataatttca
    15481 ttaactggta catcagtaat ttatcaacac ttagattggt gtggataaat tttcttccct
    15541 ctagagtgta gcaattcgct aaatacacct ttgccattgg ttttctgtgt tggatatatc
    15601 caacagggaa aataggttcg ccctcgccag caacataccg aagcatttta gatttaccat
    15661 atcttgttgc ctcctttgtc gtaagttgtc tgcctgtttt tctcaatctg ccccttttgt
    15721 ttgtacctaa tctgttagtg aagatagcca ttacagctct attcagtttc atacaatcca
    15781 gatttacatt cgttgcaatc tgataataat tttgcatacc taataccatt gaattataaa
    15841 gtcttgtttc ttctaattcc gttttccctt ttgagggaga agcaattttc tttgcctgtg
    15901 ataccagctt gtctttttgg tttttgaatt gtttatcact tatatgtgat ttaacaacta
    15961 acttctttct tctcggtatc attttaatct tgaaaccaag aaagtcggag tatctttttc
    16021 taacatttac aattcttgtt ttttcttgtg atacttccaa ttttaatcgt tgttcaatcc
    16081 attgagtgat tgctattttg gctttttcag cactttcctt gtagcggcag aaaactctaa
    16141 aatcatctgc ataacgaacc atatacattt cctttaactt tgttttcttc atttctttgt
    16201 aagcgttgct tttacatggg ctaccgcttt tattgatatg aaccacatag ttttttgtaa
    16261 caggattttc ctgccattgg ctttctaccc aatggtctaa ctcattaaga acaatgtttg
    16321 ctagtagtgg ggatattatt cccccttgcg gagttccttt ctctggatat gttattgtac
    16381 cgttagtcat tttgattgga gcttttaata tcctttttaa aatgaatatc aagtgcttgt
    16441 cccgtattcc catagcccat atctgtttta tcagcttgga atggttcacg ttgtcaaaga
    16501 aacccttaat atcaaattca atcacataat gtagctggct gatttgtagg cggttatagg
    16561 ttgcttttat tgcattttca actgacctat taggtctaaa accatagctg ttttcactaa
    16621 atttagcttc gcagattggt tctaatactt gttttataca ttgttgtata agcctgtccc
    16681 acatacaagg aatacctaga ggtctggttt taccatttgg ttttggtatt tcttttcgcc
    16741 tcactggctt aggtcgatac ccatgtggac tgcctgcaac aatgtatctt acttttttga
    16801 caactgtttc tgctggcaat ttgccaatat ctttaattat tgtaccgtct gttcctgctg
    16861 ttttactgcc tgcatttgct ttgatatttc gatatgccag taatatattt tctctcgata
    16921 ggataatatc cataagatga gtgaaaatat ctccattctt acttttggca tataaatcat
    16981 caaaggtgtt ttgcatttcg taatattcag cgtgtcttaa atcttctaca cacaatgttt
    17041 catttttatt tttaggcata aggcttcact cctttacttg aaattgagtg agctttttta
    17101 tcttactcgt aatccttatt gaaacaacat aatacttttc taatatatac acgacttgag
    17161 ggcattcctc cattctcatt acaagaattt cttaggttcg ccctctgctt ttcagcatta
    17221 gttcaactgc ttattgttct tcgtcagttt attctcttga tgagaaccca atacctttcc
    17281 tcgttccgat aatcttatct atacatatat atccttaggt cactagctat aagcctgctg
    17341 gcttgatacc gcctgtaacg ttatatggca tttcataacc tatattctta ctttgccaca
    17401 ccagctacga tttctcgtac ctttgcattt ctacaaagtt caaatttaga cccgtacatt
    17461 cgctgtttcg tcagttcctt atagaacatt ctaaccatag atattttata gacccccgac
    17521 ctgttggata cgctacccac ctctggatag ttttcgatag aagatatatg ttcatcaact
    17581 attacggtat ctttcagccg tctttaccga gcttcaaaca ttctgagttt cctcatacaa
    17641 tgcttgtcgg aggctcaggg caggcgtttc agagcgttac ttcatcattc cacctgtcag
    17701 attatcagtt ctcctaataa ccaaactttt tcgttctttt attagtgcct tagcttttca
    17761 ctaaggaacg agtcgcacat aaattttgtc cgaaattcat cttgtcactt tcctttcttt
    17821 tccattgctc gatccgctgc tgcacttgca ttcatcaagc acatcacagt aacaccaagc
    17881 atactgccac caaataccaa aaaaatatct tttacaattc ccatgacgat tcctcttttc
    17941 atataatcaa attttcaatt gccatacttt gttgctccaa aatcttttta tgtcggtctg
    18001 ataagcgtgc attctgtacc atatcctgga tataggttgt ccgattcaat cgatccaact
    18061 ccttagccat tttccacgta ggggcgactt gtcggcctag ccaattcaag gttcgttcaa
    18121 tggtatacgg ttttggctcg gaagttaatt gaatctcttt ccgattccgt ccaataaaac
    18181 gcccccattg ttcattcaag ggccagttgg ttcgcctttt accttctact ttatccgcaa
    18241 atcgcaagta acgattgata atggaaaacg tggtgctctc aatgctacga ccttttaaca
    18301 aatcttgaat cgcatgaaaa gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg
    18361 tctcagtttg gtctaatgga atccctaact tgatatattg ttcatagtct ttttcataca
    18421 aacagaaata aacctcactc ttgagactgc cgatatataa ggtattgccc atgccgtcct
    18481 tttcatcagc ctttaaaagt tccccggaac gataactttt aaaggtacga aacaaggaga
    18541 tacattcttc cttctgacat tttttcgtta gctcaggtat atccaatagc cccactcgat
    18601 cattgattgc caagtccaag cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa
    18661 agtcgtacca gctacgcttt tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc
    18721 cctttaattc taaaagcaca cctttatctt cttcatgaga caacatcacc acaatatcac
    18781 ccatgacata ctgttcttga taggagtaaa aggcataatc ttcatggagc atatactcaa
    18841 atttcaaatg gagaatttct tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc
    18901 ttacatagtc aaacagtaac gtcaaaggca aatcgggatt aaagctttcc agttggtcaa
    18961 aaattttcct catggttttt atagatgcgg ttacgccacc attttctatc tgatttaagt
    19021 acggccgact aagtccacaa gccgtagcca atttattttg tgaaacgccg tattcgttgc
    19081 gtcggtcttt taatagccga taatctaaat tccgttgtgc caagttctca cctccaaatt
    19141 caaggtgtta actttttcaa atgagttaac acctatacaa attacttact atctttgata
    19201 caacaagcat tcttaatggt tgcttactta ttttttgtgt ttttgtaccc ccctgttaga
    19261 tacgggggtg agacatcatg gtcggctaac gccgtccatg aacaggcaca gcaccacgga
    19321 ggatttctgc ttcgcgaatc ctccgcggtc agcgcctaac agtcagttgt gttggtttag
    19381 gttactcatc tgtaatacca tttacttcca actctgcttt catttctaac aagtattgac
    19441 gaatatctgg ttcttttgct tggtccgcaa tccgctcaaa ttctgcaatt acttgattta
    19501 tttcaattcg agcatccgtc tcctcattca ttaaatatgg atcgccagcg actttgacac
    19561 cattcttgcg aatatcaaat tttaaccgtc gcaattgttc ttctaattct gggtctgact
    19621 gaatctttcc aatcacccaa taaatttctc caatcaactc atcataaacc atgtacatcc
    19681 aactctttta atcttctggc aatatcaaaa atgaccggaa ctgttacaga gtttccggct
    19741 tgcttataca actgactatc tgaattgact tgagaatctc gatcaaaagc ccaatcggga
    19801 aagccctgaa gtcgccaaca ctctctaggt gttagcttcc gaatttgata ctgatccgtg
    19861 acgatcgctt gttggtctcc tgttagtaag gtatgaacac tctgttttcc cagacgacct
    19921 cggcgacttg tggaattagg aaaagccaga ttcacactgt cttctggctt cacctcttga
    19981 tattctgttt cattttttcg aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg
    20041 ggggcggttc cagtaacttc ataaactcga ttaccttgtt caaactttcc cggtaatagt
    20101 cctgctacag agattccatc ccttgctggc agtgcgattt tgattccttc atcacctgtt
    20161 aaggttggcg ctacgccatc tgaaccatag acttgtccac ccattcctcg attggaagga
    20221 ttgatattta ccaaatttct gatgttttga tcagatgttg aagacggtcc aatgatagga
    20281 aatacttgtt cggtacatgc gtctcgagaa tgtccgataa ggaatatccg ctcccggctt
    20341 tggggaatgt aggcctttga gttaagcacc taccactcca cgtcataccc caatccatcc
    20401 agcgttcgga ggatcgtctc gaacgtagcc cctccttcgt gattgagcaa tcctcggacg
    20461 ttctcaagga ataaaagctt aggtcggaga agagtggtga accgagcgat ttcaaagaat
    20521 aaagtacctc gagtatcgga aaatctttgc cgctttcccg caattgaaaa agcttggcac
    20581 ggaaatccgc cgcaaaggat gtccactggt ccgagggatt gaataaactc atctgataca
    20641 cttgtgatgt catgcatttc cacctcctta cttgtatcat gaattgcttt ataactacgt
    20701 ctagcaaatt tatcaatttc acaaaaaccg atacaatgat ggctcgcctg ttccatacct
    20761 aaacgaaagc caccaatacc ggcgaataaa tctaaaaatc tcatacatta cctcattcat
    20821 tgcaaagaaa aaagccaaga acattttgct cttggctcat acattattaa tttgtcctac
    20881 tattttttat ttaatccaac taatttccgt tgtttttcat tggcctgatc ggctacatat
    20941 ttaatcaccg ttttgaactc ttttgcagta gcattttccg ataaatccgc aatgctctta
    21001 ttgtatttct ttacaatttt cgttacttgt tctttctcag tcatgtaatt gaacctccaa
    21061 atctttccta ctatacccta gtggtaaaaa taattctaga ttatccgtag cataatgaac
    21121 ttcggaaata ttattcgtat aatcttttat tatgatatga agcaatacat ctggatgttt
    21181 actctttact tttgcaatgt tctctcttga tttgaaaaaa gcgttaataa atgccttttt
    21241 cggcacatga cgtccctctg caatttcacg ttcttgtgta aatttccaag cgattttagg
    21301 gtcttgataa acatagaaaa tcgtcactcg aaaatgtttt ttcaacgccc gcgagatatt
    21361 tttttcagca cttaatattg caaaagttgc atccagaata aaagaatagc catgatctaa
    21421 gacatatcta aatgcctgct ctaccaacca agaagatgct ttttgaaaga gactagagtt
    21481 ttttccatta taatcaggaa actgggtgcg aaagtaatcc gcatcaataa tcacatggtt
    21541 atcataattc tcagctaatc caatggcaac ctctgatttt ccagccccag gactccctgc
    21601 cataaaaact gcgtctcgtc caacttcggg ggatttccct ttgataatat aagaaataaa
    21661 cttatcctta tttgcttttg catactctaa ataagaatca ttactttcca tattaattat
    21721 cccctttctt cctactatat aaaagtatta tacgttactt gaagtataaa gtcagtattg
    21781 aagatcactc aatcattcat cttttctaat ttactaattg aatctaagaa atcataccct
    21841 tttggtacaa gtggcgtata aaactcacta atcacactgc cacctgtatc cacataacct
    21901 cgacctttga tgtgcttcat aaagaagttt ttttcgacct ccccaaacat catggaatat
    21961 ccgagttcac tcatacgacc caaagccacg cgaaagttga attgatcgcg gattccgtct
    22021 cctaaatatt tcgcatccgg cctttggcaa gccaaaatca gaaagaaacc agattgacga
    22081 cccaacatca cgatttgttt gagcttattc aaaatcaccg cactttcttt cgtcgttaac
    22141 atctccatat aagccacgta ttcatcaaag attaaaaagt tgggtggaag tccaagatag
    22201 gcataattct ctcctgtttt gtagttagac atttctttca ttgccttgct acgagccatc
    22261 atacgttcat agaaatcttc cacacaagca gaaatttctt ccttttgtga ataaacatga
    22321 ggcatcaccg tacctaaatc agctaaatct gcatttttgg gatcgaggat aaacaattct
    22381 gcatctgact tcaatagtgc ttcgataatg gtgaggagga aataggtctt ccctccaccc
    22441 gtgccaccag cgattaacat atggggtaaa gaatcataag cccatacttg atttttcatc
    22501 aatcgcagag tcccattttc tgccactact tcgtctatcc caattcgatt ggcgatcata
    22561 tcatacagta aagtatactc cacataggag tctttgagtt ccttttctac taactcacaa
    22621 tacagtccgc tttctaactt cttttccaat tttaaaagct gatcttggta tttcccaagt
    22681 gaaatttgaa cgcgaataga cagtaatcca tctttcattc gataacagat tttggggaag
    22741 taactgatag tctctctggt tcggctacta ttcaaatctt tgaaaaagcc ttcactttga
    22801 gtttgtttca cttcatacca atggttctct agaatcatcc gagctagctt ttgtcgatgc
    22861 cacaagcttc gccaatgatc aagaaaaaag tgaatataaa gcaatatcat agctcctgaa
    22921 aaagcgagac ttccaagcag acatactaaa tcttttatag accaaacgat ttcaatagta
    22981 gaaagtgata gcttagaaat atcaaattgt ctaaactctt gaaaataaaa gacgctcatc
    23041 caaccgatga acagagcgag tagccagcca agaacaaagt gataaaccaa gtgctgatct
    23101 cctgcgcgta tgcgatgtcc tttgtacatt ttgatggttt ccacctccaa tcgaaaaagc
    23161 ccaataggat ttcattaggc aatttcaaaa agttatgcta gtcatttact gctacgagga
    23221 ggggagttgt ttcgtttcaa ccaattgatt taaaaagcct tcaatagcat ccggcatctc
    23281 cacaccaatt tcttccaaaa tggattctgc ctgccgacat aagttttcat ttacttcgac
    23341 aactataaag aaagactctt cttctcctaa ccagtctagt acatcagagt cttcttcact
    23401 gtcgctattt tctccaagta aaccttgatg tataatcaga aacatttttt catcagaggt
    23461 taatgcttta acgagttgtt cctcagtcag ctcgcctttt ccatattgat cgagacaatc
    23521 taaaatatgt tcaacattaa tttccataat cttcacctat ttctttcttt cattttggtt
    23581 cgtaggattt ccttgattcg gctgccgctt caagactaaa tcatccgcct taatgaacca
    23641 attaacggta gttccttctc gaaaaacata attggcaacg gtatccacga ttggattgac
    23701 caactctacc tctgcatcat aagcaaactc gcgtaagggt acattagctg ggatactaac
    23761 ttgaatcatt cgcccttgtg cagaggattt taaattatag gttcgttctt ttacctcatt
    23821 accaattgtt ccatcctcat tttgcttccg tacttctcgg cgtaatgcgg aaaaacgcaa
    23881 cttcccaaat gttaaacctg aatctacgac aataccttct gttaatctca tcttcttttc
    23941 ctcctatgct ttcactaaat catcaacata caacacgtaa tcagtaaacc cacgattctc
    24001 aatctcatag cctttggcaa ccaaatgggg attcacggct tttagcggtt ggtcttgatc
    24061 aaaatccttt tcaccggctt ccgcagctac taccacctca atatcatcgg cccgttgctt
    24121 actggaatac aaatgataac tgcgaccaat caccgtagta ttccgtccat atccttctgt
    24181 tataatttct ccttcaccgg cataacttaa ctttccaaag gtttcagcca tatctggcac
    24241 gacaaatttc aattccatct tcttttcctc ttttcattgt tagttttgga tacaaaaata
    24301 gcgcgactac ttttttagga ggcggtcgcg cacgttggtt cttaggattt acttgtatca
    24361 tctcatcacc ttagttccgg cgattccaga aataatagcc cgccgtcgca aaaatcaaag
    24421 taaaaccaat gaacagtaaa acattggaat ttttctcccc ggtttgaggg aaggtcttca
    24481 ctgtgggact agattcttga gagccacttg gaacagttgg tgtttccggt tgtttcggcg
    24541 tacttagtat ggttggaact tcttttggtg ttaaggtttg agatttttct ttcaaatctt
    24601 cattgtgttt tccattgata ttcccatctt tatcgtaatt gatttccgca aaagtaaact
    24661 tggttccttc tggatatttt ccggtatcta cttttttcgc aagcacagtt ttggtgaatt
    24721 ctttatcatt tacttcataa tcaatcttgc cagatttcca aatttctttg tttgtaccat
    24781 ccggtaataa agcatacaga attgtttcga aagcttcttt tgagccatcc agtacatcat
    24841 gagtaatcga cacatcatca aacatatcca ccacatcacc atgagtaaag gtctgagaac
    24901 cgttttctag gtgggccttt gtttggatag aaacatggtg ttccactgta caactgacag
    24961 tttgggcttg gttattcaag ctaacatcct tcgctactgg cttatcgcct gcttcaaaag
    25021 cttccttatt ttcatacaca tagttgaata agacaatagt tttatttaaa acttgttcgg
    25081 cggttaattt atgctgaatt ttccaagttc ctgttttctc tttgttcgcc aacgttgcgc
    25141 ttgtttctgc aatcaccact ggtttggctt tttcatcttt ttcttgggca gcttttgtcg
    25201 catccacatc aatggcttgc gctaccacat accagtcttc tttgatttca tgtagattgt
    25261 aactcaattt atcaactagc tcggtagaat ccagagaagt taattctttg tttccgtctt
    25321 tccaagtcgc aagagaagtt aaactatctt cttcctcagg caaatcatag agcattaaac
    25381 ggttcaaact aacagaaaat gctttgttcg tcagcttctc gtaaggaacg gtcaccgttt
    25441 taattggttg tttttggtct tgttcggtaa tggtaaagac atactcactc ttcacaaagt
    25501 cttctttgtt ttccttaaaa gtagaacgga tttctaatgg cgtaatcttt tgaaaacctt
    25561 ctggggcttc tacttcttca agtaaataat ccccataagg aagattttca aacttgccat
    25621 aaccatcaaa ccctaattgc tcgttataag ctgtggtcgc ttcatcttcg gcacctgtga
    25681 tttcattggt cccttccaat ggcgacactt taaaggtcag atcattgaag ccagtttcgg
    25741 tagtgcccgc agctgatcca gcaaatttaa agaaatcaaa gccaaagcga atcacttgtt
    25801 cttttgccgt aacatttcgg gtaatcacgg catttttttc gctatcatcc acctttttga
    25861 tggatacagg atactttgtt tcatcaaggc tatatccttc gggcgctttt gtttcttgcc
    25921 aataatactc gttgatagct aaatgtttaa cggcaacttg gttcttttcg tctaaagtca
    25981 aagtgaccgt ttcatcagaa cctttcgttc ccttcgctaa ctctggttta aaagcctcac
    26041 tccatttaac agcttttcca tccttagcag taaacaaggt gtattcagca ccctcaaaga
    26101 ctgccttacc ttgtgcttta tccccagtat ctttgtcttc tttcgtcaaa gtggtttcgc
    26161 cagtgacttc ttgattttgc ccttttacgt tactggtaac aggggctacg gtttgattgg
    26221 catacttcaa ttcgactttt acaggtttga acgtattcac gaacccatga ctagctttag
    26281 tttcggtcac ataataggtt cccaattcca acgcattagc aatctctttt ggtgtttccg
    26341 catgaccgtt ttcatccgtg gtcatttctt gaacgatttc accggttgga ctgtctttgc
    26401 gaatggcaaa cgtattacca gctaaggaat aattgtcatt ccaaagatca ctccctgttt
    26461 caactccgct tttatccaga atgatttgcc ctttttcccg tgcgtttttt gaagtcacgt
    26521 aaatggtttc gcctgcttta atggtggtag tcatgggggt ggtatcaatc gtataaggcg
    26581 ccggtactga tttttcggta atggttactt ttgtaccatg aggaatctca tccaatgtag
    26641 caatgccttc tttgtcagta gtcacgtctt ttgcaggtaa agtttttcca aagtctaaat
    26701 ggaaaaccgt tcctggtaca acatcacccg attctttatc gacttttttg atctttaaag
    26761 aaccctccgt ttccacatca attttgattg tgtaggtatt aggtttatca atcgccccgg
    26821 ccattaaagt ctgttggcca gcctttttat aggcgacagg tgttccggta ccagcagatt
    26881 tcttaagcgt cagcacacca ctttcttttg aattggcatt tggggtgatt atcaactgat
    26941 taccattcac acgataatca atgtttgcgg cattttccac aacctcatcg aactctgaca
    27001 aattcagatt attcgtatcc gtcacagtag tcgattgccc taatactgtt ttagccgtac
    27061 tattatggaa ggttggtttc ttctgataat cgtcaatggc ttgatttatt tttgcctcaa
    27121 tggctgcgat atttacggca ctaccatctg atcgttttat tgagtgaagg gtatacccat
    27181 taacttcttg ccagatcatc ttctgtgcca caatatgagt atctgcatct gtacctactt
    27241 ttttccataa aacggaaact aatttggctt tatcggacat atctggtaac gggtttttct
    27301 catagccagg agtgaactca ttgtaaatcg ggactcctgg ttcaatacag aaaatatctt
    27361 gtttaaagcc gttgtacact gcataatgcg gaacagtgac ttctgacatt ggacgaccat
    27421 cactaaaagt tccttcaaca gcatattgat gtgctaaatc tagttctacg gttaccgttt
    27481 gtggatgggt aatttcatca gcaaaagtaa tcgtaggagc aattgctcca acaattgctt
    27541 gaacagccaa actcaaaata gttaatacac ataatttctt tttttgtttt ttatacatga
    27601 aatgttctcc tttcaaatta agcacaaaaa aataagatgc tcgcttgaac atctttgttt
    27661 ataaagtctt tttaaaattt ttattgactg aatagaatgg atttgagaat gaatgagcct
    27721 aaaaatttaa tctaaaatac gaaatatact gcctttaaat tagaacacat tttagaacac
    27781 aaacatttca aatttaatca aatataataa aatatattta cgggaaaacc tttataaatc
    27841 aaggtttttc aaacttattc aatgatacga agaaaaaacc aggtcttaag aaagcccgta
    27901 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
    27961 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
    28021 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
    28081 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
    28141 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
    28201 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
    28261 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
    28321 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
    28381 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
    28441 tacaaggacg acttatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
    28501 tagcactgtt tggtgcgctc tttgattgga agacaacgca accacgtctg cgagacagtc
    28561 taatttttct gggagggagt cttgctatag ttatccttat aaccggtctg gtaaaattac
    28621 tcaagcttga tctgaaagaa gcaaaaattt tcagtgaaga ggaatacaga gaaacgactt
    28681 tggtcgaaaa gaaagaatca ggaaactgat acaagcgtat aacaaactgc ctgataatag
    28741 gaaagacacg tattgtccga ttgttttttt tgacattcaa ttggaaaatt tacactttct
    28801 ttatacattt ctggctgctt tctttatacc cgtcaactat acttcactca tcaataagaa
    28861 agaaggataa gtatggattt tggaaatgaa acggtcttga aagcaactgg tataaccaaa
    28921 aaatacggtg ctgccaaagc attagataaa gtatcaatcg aaatcaaacg tggaatgatc
    28981 tatggattga ttggggaaaa tggagcgggt aagtctacct ttatgagaac tattatgggt
    29041 ttgatctcga ttgatgaagg ttcgatcgaa ttatttggaa cgacggattt gcaggcagca
    29101 agacgacgta tgggtcaatc gattgaaacc cctgctcttt atccagaatt aactgcaaga
    29161 gataacctac gtatccaagc agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg
    29221 ctgaaaatga tgcgattgga aaatacaggg aaaaagaaag caaagaattt ctctttaggt
    29281 atgcgccagc gtttagctat tgcaaatgca ctgatcacga atccagaatt tttgattttg
    29341 gatgaaccga cgaatgggat ggatccagca gggatggcag aaatgcgaga aatcatccaa
    29401 cgtttggtaa aagaacgggg catcaccgtc ttgctttcta gccacttgct agatgaattg
    29461 tcacagatcg ccactcacta tggtattttg catgaaggac acttgatcaa agaattatcg
    29521 aaagaagaat tggcacaaga atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa
    29581 caagcagtaa cggttctcga ctctcttggt tatagggatt attttgtaca atctagccgt
    29641 gttatccagc tttttgaagg aattgaccaa gtagcagcca tcaatcaagc attggtagaa
    29701 gcaaaagttc ctgtggatgg tattcaccta gttgggcaaa aactggaaga ttatttctta
    29761 caattaactg gaggcaatcc acatgtttaa tctattaact gctgaaaaaa taaaagtatg
    29821 gaagagccgt aaaatgtggg tctcactagg actgatggtc gtacttccgc ttttttattc
    29881 ttggaacgaa tggtatatgc atacaaaata cggcaatgaa ttaaaccaag caacggatac
    29941 agtcatcaac ggtgcaacag ggattttaat ggtagaaaaa atggctgcat ggattcttct
    30001 ggctttcgca gcatttgcgt gcttttatat tggggaagaa tttcaaaacg gtacgattcg
    30061 caatacgtta tctttaggac ggaatcgaat gacttattat gtgtcgaaac ttctcgttac
    30121 gcttttgatc acaacacttg gcgtggtttt gatcacggga ctagccatgc ttgcttacac
    30181 acttgctttc ggatttggtg aagttgaggg aatcaaaaac tatggaaatt atgttctaaa
    30241 agtatttcct gtattgcttc tgctgacatt agggacatta tctgttccag tggctttgac
    30301 attcatcact agaagtacca gtgtcagttt gcttctttct tttctttata tcatgggaac
    30361 ggccttcatt cccggtgttt tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt
    30421 tacggaaacg tggctcatgt atacggactt tgcgcaacaa gcaacttatt cgcaagcacc
    30481 gaaaatgatt ctggtcagtc tagtaacaat cgtggtatca tcagctctag gaatgtttat
    30541 tttccaaaaa tctgacatta agtaggtggt cctgctggaa actattttga ttatcgaaga
    30601 cgatgaagct gttcattcct tactggagga ggttcttgaa cagagataca aacttcttga
    30661 tgcttattca ggtacggaag gaaaattatt gttaagcaca tatcctgtcg atttgatcct
    30721 tctagatctc atgcttccag ggctttcagg tgaagcattg ttagcagaaa tccgacagac
    30781 atcgaatgtt ccgatcatcg ttctttcagc aaaaagcgat caaagagata aagtttcctt
    30841 gttggcagca ggagcggatg actatgtgac gaaaccattt gatatcgaag aacttttgtt
    30901 acgaatcggc atccaacttc gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg
    30961 catctattac aaggaaatct tactggacaa agagtcgagg gatgtaaatg tgaatggtgt
    31021 ggctgtccat ttgacaggaa gagaattcga tctgctgaag ctttttttag aacatccgaa
    31081 aaaagtattt tcgcgagcaa atctttatga aacagtttgg caagaaccat attttgatag
    31141 tgagaaaaca gtgaatatgc atatttccaa tcttcgaggg aagctagcaa aggcacatgc
    31201 tgtgtatatt catacggtgt ggggagtggg gtttagattt gattaaatgg ctgttgcttc
    31261 tcgttgttat ctttatggga agttttattt tcttcctgca aagggaaatc aagagattaa
    31321 ctaagaaaat cgccactctt tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat
    31381 ttcgagaaaa gaatctaatg gacctagtag atgcactgaa tacatggttg gatgaaaatg
    31441 aacaaaaagg acaagcattt caagaaatgg aagaaaacat cagactatcg atcgtcggcc
    31501 tttctcatga tctcagaact cctttgactg ctattaacgg ttatgtacaa ttactggaac
    31561 aggtagagga tacggagaaa cgggagaagt atttaacgat catcaaacaa tctgttgcaa
    31621 gattactcag tatgacggat cagttttatg atcttgctcg aatagagacg aagcaaaaag
    31681 aaatggatct ccatacgctt tctttttcta aagaggtgga agaagtattt ttctcttttt
    31741 atgaacaatt ttctgagaaa aatatcgagg tagcgttcgc tgaacactcg ataaatacaa
    31801 ctgtattagc agatccgctc atgttgctgc gagtgctgca aaatatcata cagaatttat
    31861 tgcgttatgc agatggacaa gcaaaggtga cattttcaaa agaggatcca ttcattcgat
    31921 taacagtggc caatcctatc aaaccaggaa cgcaaacctc tgttgaaaaa gtcttccagc
    31981 gtttttatac cgaaaatacc agccgcacaa atacggaatc tagcggaatc gggctttacc
    32041 tgtcaaaaaa attggtggaa ggaatgggag gagagatgac cgctagatta gacgggggca
    32101 tcttctccat ctccgtcaag ttgcggaggg tttaattata caagattagt gagaactagt
    32161 agaaaaatgt attatcttat atatatttaa tagagaaaaa agcctgtgat ataaatattg
    32221 caggcttttt tattgatttg aaataatcaa gaatattaga aacttctaca ttaatcaaga
    32281 actttcttct gtatgaaaat agagataata attgtacact taagctgtat tcaggaggtg
    32341 aagataatgg gcatcgaatt tagcgaacgg caaatgaaaa tactatactt tttgattgaa
    32401 aatgagcaca caacttacaa agaattatca gaacaattat atgtatctac gaaaacaatt
    32461 agcaaagata ttttaatttt gaaagaaata atccaaaaaa tgccagatgt acaaatgttg
    32521 gttcaaccta aaaaaggcgt ttcgcttatt ggaaaaaaag aaacacttcg aaatttatta
    32581 tccagtcata aaatcaatat cgcagactct ccggaggacc gtctaactta tgtgtttgct
    32641 aaattattga atactaatga ttctctaaag attcaaaatc ttgctgacga attatacgtg
    32701 agcaggtcga cggtagaaag cacaatgaaa gatgttagaa aaagactaaa caagcatggg
    32761 atgtccttag tttcagatcg aaatggcgtg cgcatcaaag ctgatgaaaa aagtaaaaga
    32821 aggttgatgt cagaagttgt gaactattat tggggtggct ttacagcatc taacagtaaa
    32881 acagatctct tagacttgaa tatcaatttc attggtgaag cgaataattt cttaaataag
    32941 gacatattga aaaaagtagc cgaattgctt aatcgataca tgaataagac acaattgaaa
    33001 tttacagatt atgaatttca aactttagct atccaccttg ctattgcgat agaaaggatt
    33061 gaaacaagca atttcttatc acgtagtaat aattctgaag aagaaatctc aaagaataca
    33121 cttattttaa tcaaaatgat
//
LOCUS       DAYYCM010000021        32793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000021 DAYYCM010000000
VERSION     DAYYCM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.022"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHAY1_001141"
     CDS             complement(29..1261)
                     /locus_tag="ACHAY1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8593148.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHAY1_001142"
     CDS             1516..2085
                     /locus_tag="ACHAY1_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593149.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHAY1_001143"
     CDS             complement(2263..2703)
                     /locus_tag="ACHAY1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8593150.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHAY1_001144"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHAY1_001144"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8593151.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACHAY1_001145"
     CDS             3657..4580
                     /locus_tag="ACHAY1_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8593152.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACHAY1_001146"
     CDS             complement(4805..5044)
                     /locus_tag="ACHAY1_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593153.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACHAY1_001147"
     CDS             complement(5049..5468)
                     /locus_tag="ACHAY1_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8593154.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHAY1_001148"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHAY1_001148"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8593155.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACHAY1_001149"
     CDS             complement(7040..7675)
                     /locus_tag="ACHAY1_001149"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593156.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACHAY1_001150"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACHAY1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACHAY1_001151"
     CDS             complement(8017..8652)
                     /locus_tag="ACHAY1_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8593157.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACHAY1_001152"
     CDS             9164..10306
                     /locus_tag="ACHAY1_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8593158.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACHAY1_001153"
     CDS             10299..10505
                     /locus_tag="ACHAY1_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8593159.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACHAY1_001154"
     CDS             10584..11777
                     /locus_tag="ACHAY1_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593160.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACHAY1_001155"
     CDS             11982..13121
                     /locus_tag="ACHAY1_001155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8593161.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACHAY1_001156"
     CDS             13114..13914
                     /locus_tag="ACHAY1_001156"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8593162.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACHAY1_001157"
     CDS             13914..14741
                     /locus_tag="ACHAY1_001157"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8593163.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACHAY1_001158"
     CDS             14746..15453
                     /locus_tag="ACHAY1_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8593164.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACHAY1_001159"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACHAY1_001159"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8593165.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACHAY1_001160"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACHAY1_001160"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8593166.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACHAY1_001161"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACHAY1_001161"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8593167.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACHAY1_001162"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACHAY1_001162"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8593168.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACHAY1_001163"
     CDS             19038..21071
                     /locus_tag="ACHAY1_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593169.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="ACHAY1_001164"
     CDS             21115..22395
                     /locus_tag="ACHAY1_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8593170.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="ACHAY1_001165"
     CDS             22605..23411
                     /locus_tag="ACHAY1_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593171.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="ACHAY1_001166"
     CDS             23423..24643
                     /locus_tag="ACHAY1_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593172.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="ACHAY1_001167"
     CDS             24633..26219
                     /locus_tag="ACHAY1_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8593173.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="ACHAY1_001168"
     CDS             26258..28396
                     /locus_tag="ACHAY1_001168"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8593174.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="ACHAY1_001169"
     CDS             complement(28764..29747)
                     /locus_tag="ACHAY1_001169"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8593175.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="ACHAY1_001170"
     CDS             30125..31516
                     /locus_tag="ACHAY1_001170"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8593176.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="ACHAY1_001171"
     CDS             31532..32572
                     /locus_tag="ACHAY1_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8593177.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..>32793
                     /locus_tag="ACHAY1_001172"
     CDS             32591..>32793
                     /locus_tag="ACHAY1_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8593178.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKK"
BASE COUNT        10549 a         5696 c         6482 g        10066 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaa
//
LOCUS       DAYYCM010000022        31457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000022 DAYYCM010000000
VERSION     DAYYCM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.023"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHAY1_001173"
     CDS             212..604
                     /locus_tag="ACHAY1_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8593179.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHAY1_001174"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHAY1_001174"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8593180.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHAY1_001175"
     CDS             2698..2943
                     /locus_tag="ACHAY1_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593181.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHAY1_001176"
     CDS             3165..4775
                     /locus_tag="ACHAY1_001176"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8593182.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHAY1_001177"
     CDS             complement(4752..4964)
                     /locus_tag="ACHAY1_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593183.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHAY1_001178"
     CDS             5067..5936
                     /locus_tag="ACHAY1_001178"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8593184.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHAY1_001179"
     CDS             6101..7372
                     /locus_tag="ACHAY1_001179"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8593185.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHAY1_001180"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHAY1_001180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8593186.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHAY1_001181"
     CDS             8763..9026
                     /locus_tag="ACHAY1_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8593187.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHAY1_001182"
     CDS             9471..9920
                     /locus_tag="ACHAY1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593188.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHAY1_001183"
     CDS             9977..11395
                     /locus_tag="ACHAY1_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8593189.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHAY1_001184"
     CDS             11395..12255
                     /locus_tag="ACHAY1_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8593190.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHAY1_001185"
     CDS             12429..13202
                     /locus_tag="ACHAY1_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8593191.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHAY1_001186"
     CDS             13349..13714
                     /locus_tag="ACHAY1_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8593192.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHAY1_001187"
     CDS             complement(13862..15070)
                     /locus_tag="ACHAY1_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8593193.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHAY1_001188"
     CDS             complement(15089..15367)
                     /locus_tag="ACHAY1_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593194.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHAY1_001189"
     CDS             15560..16216
                     /locus_tag="ACHAY1_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8593195.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHAY1_001190"
     CDS             complement(16658..17455)
                     /locus_tag="ACHAY1_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8593196.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHAY1_001191"
     CDS             complement(17452..18636)
                     /locus_tag="ACHAY1_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8593197.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHAY1_001192"
     CDS             complement(18661..19908)
                     /locus_tag="ACHAY1_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8593198.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAY1_001193"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAY1_001193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8593199.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHAY1_001194"
     CDS             21888..22202
                     /locus_tag="ACHAY1_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8593200.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHAY1_001195"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHAY1_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8593201.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAY1_001196"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAY1_001196"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8593202.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHAY1_001197"
     CDS             23546..23875
                     /locus_tag="ACHAY1_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8593203.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHAY1_001198"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHAY1_001198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8593204.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHAY1_001199"
     CDS             24863..25687
                     /locus_tag="ACHAY1_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8593205.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHAY1_001200"
     CDS             25680..26027
                     /locus_tag="ACHAY1_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8593206.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAY1_001201"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAY1_001201"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8593207.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAY1_001202"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAY1_001202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8593208.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAY1_001203"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAY1_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8593209.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAY1_001204"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAY1_001204"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8593210.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10112 a         5635 c         6634 g         9076 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtat
//
LOCUS       DAYYCM010000023        31089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000023 DAYYCM010000000
VERSION     DAYYCM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.024"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACHAY1_001205"
     CDS             complement(19..729)
                     /locus_tag="ACHAY1_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8593211.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACHAY1_001206"
     CDS             complement(726..2198)
                     /locus_tag="ACHAY1_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8593212.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACHAY1_001207"
     CDS             2336..2809
                     /locus_tag="ACHAY1_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8593213.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACHAY1_001208"
     CDS             complement(2962..3513)
                     /locus_tag="ACHAY1_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8593214.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACHAY1_001209"
     CDS             3618..4148
                     /locus_tag="ACHAY1_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8593215.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACHAY1_001210"
     CDS             complement(4631..5074)
                     /locus_tag="ACHAY1_001210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593216.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACHAY1_001211"
     CDS             complement(5090..6250)
                     /locus_tag="ACHAY1_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8593217.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACHAY1_001212"
     CDS             6757..8457
                     /locus_tag="ACHAY1_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593218.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACHAY1_001213"
     CDS             complement(8559..9392)
                     /locus_tag="ACHAY1_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8593219.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACHAY1_001214"
     CDS             complement(9696..11861)
                     /locus_tag="ACHAY1_001214"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8593220.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACHAY1_001215"
     CDS             12121..14415
                     /locus_tag="ACHAY1_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8593221.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHAY1_001216"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHAY1_001216"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8593222.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACHAY1_001217"
     CDS             15066..16073
                     /locus_tag="ACHAY1_001217"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8593223.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACHAY1_001218"
     CDS             16095..17114
                     /locus_tag="ACHAY1_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593224.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHAY1_001219"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHAY1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8593225.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHAY1_001220"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHAY1_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8593226.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHAY1_001221"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHAY1_001221"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8593227.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHAY1_001222"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHAY1_001222"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8593228.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACHAY1_001223"
     CDS             24539..25840
                     /locus_tag="ACHAY1_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8593229.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACHAY1_001224"
     CDS             complement(25909..26508)
                     /locus_tag="ACHAY1_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593230.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="ACHAY1_001225"
     CDS             26762..27643
                     /locus_tag="ACHAY1_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8593231.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="ACHAY1_001226"
     CDS             27656..29266
                     /locus_tag="ACHAY1_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593232.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="ACHAY1_001227"
     CDS             complement(29343..30002)
                     /locus_tag="ACHAY1_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8593233.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="ACHAY1_001228"
     CDS             complement(30285..30950)
                     /locus_tag="ACHAY1_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8593234.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9296 a         6341 c         5644 g         9808 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gagagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttt
//
LOCUS       DAYYCM010000024        30968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000024 DAYYCM010000000
VERSION     DAYYCM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.025"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..1786)
                     /locus_tag="ACHAY1_001229"
     CDS             complement(20..1786)
                     /locus_tag="ACHAY1_001229"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593235.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1746..3500)
                     /locus_tag="ACHAY1_001230"
     CDS             complement(1746..3500)
                     /locus_tag="ACHAY1_001230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593236.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3610..4179)
                     /locus_tag="ACHAY1_001231"
     CDS             complement(3610..4179)
                     /locus_tag="ACHAY1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8593237.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4197..5612)
                     /locus_tag="ACHAY1_001232"
     CDS             complement(4197..5612)
                     /locus_tag="ACHAY1_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593238.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5623..6291)
                     /locus_tag="ACHAY1_001233"
     CDS             complement(5623..6291)
                     /locus_tag="ACHAY1_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8593239.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6421..7005)
                     /locus_tag="ACHAY1_001234"
     CDS             complement(6421..7005)
                     /locus_tag="ACHAY1_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593240.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7336..7569)
                     /locus_tag="ACHAY1_001235"
     CDS             complement(7336..7569)
                     /locus_tag="ACHAY1_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8593241.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7584..8534)
                     /locus_tag="ACHAY1_001236"
     CDS             complement(7584..8534)
                     /locus_tag="ACHAY1_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8593242.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8531..9373)
                     /locus_tag="ACHAY1_001237"
     CDS             complement(8531..9373)
                     /locus_tag="ACHAY1_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8593243.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9375..10094)
                     /locus_tag="ACHAY1_001238"
     CDS             complement(9375..10094)
                     /locus_tag="ACHAY1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8593244.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10642..10944
                     /locus_tag="ACHAY1_001239"
     CDS             10642..10944
                     /locus_tag="ACHAY1_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593245.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            11016..12242
                     /locus_tag="ACHAY1_001240"
     CDS             11016..12242
                     /locus_tag="ACHAY1_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593246.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12330..12722)
                     /gene="rpsI"
                     /locus_tag="ACHAY1_001241"
     CDS             complement(12330..12722)
                     /gene="rpsI"
                     /locus_tag="ACHAY1_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8593247.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12738..13166)
                     /gene="rplM"
                     /locus_tag="ACHAY1_001242"
     CDS             complement(12738..13166)
                     /gene="rplM"
                     /locus_tag="ACHAY1_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8593248.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13700..14167)
                     /locus_tag="ACHAY1_001243"
     CDS             complement(13700..14167)
                     /locus_tag="ACHAY1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8593249.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14287..15825)
                     /locus_tag="ACHAY1_001244"
     CDS             complement(14287..15825)
                     /locus_tag="ACHAY1_001244"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8593250.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15905..16591
                     /locus_tag="ACHAY1_001245"
     CDS             15905..16591
                     /locus_tag="ACHAY1_001245"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8593251.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16792..17319)
                     /gene="msrA"
                     /locus_tag="ACHAY1_001246"
     CDS             complement(16792..17319)
                     /gene="msrA"
                     /locus_tag="ACHAY1_001246"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8593252.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17403..18080)
                     /gene="pgmB"
                     /locus_tag="ACHAY1_001247"
     CDS             complement(17403..18080)
                     /gene="pgmB"
                     /locus_tag="ACHAY1_001247"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8593253.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18077..19207)
                     /locus_tag="ACHAY1_001248"
     CDS             complement(18077..19207)
                     /locus_tag="ACHAY1_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8593254.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19207..21390)
                     /locus_tag="ACHAY1_001249"
     CDS             complement(19207..21390)
                     /locus_tag="ACHAY1_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8593255.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21387..22412)
                     /locus_tag="ACHAY1_001250"
     CDS             complement(21387..22412)
                     /locus_tag="ACHAY1_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFM8593256.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANTSVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22457..23284)
                     /locus_tag="ACHAY1_001251"
     CDS             complement(22457..23284)
                     /locus_tag="ACHAY1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8593257.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23281..24348)
                     /locus_tag="ACHAY1_001252"
     CDS             complement(23281..24348)
                     /locus_tag="ACHAY1_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8593258.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24374..25210)
                     /locus_tag="ACHAY1_001253"
     CDS             complement(24374..25210)
                     /locus_tag="ACHAY1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593259.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25207..26076)
                     /locus_tag="ACHAY1_001254"
     CDS             complement(25207..26076)
                     /locus_tag="ACHAY1_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593260.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26146..27429)
                     /locus_tag="ACHAY1_001255"
     CDS             complement(26146..27429)
                     /locus_tag="ACHAY1_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593261.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27443..29128)
                     /locus_tag="ACHAY1_001256"
     CDS             complement(27443..29128)
                     /locus_tag="ACHAY1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8593262.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29403..30428
                     /locus_tag="ACHAY1_001257"
     CDS             29403..30428
                     /locus_tag="ACHAY1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593263.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9445 a         6198 c         5049 g        10276 t
ORIGIN      
        1 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
       61 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
      121 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
      181 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
      241 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
      301 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
      361 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
      421 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
      481 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
      541 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
      601 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
      661 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
      721 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
      781 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
      841 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
      901 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
      961 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
     1021 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
     1081 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
     1141 tctcttgctg caattctata gtttcttgaa attcttccga gttctctctc aaagtatcgt
     1201 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
     1261 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
     1321 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
     1381 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
     1441 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
     1501 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
     1561 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
     1621 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
     1681 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
     1741 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
     1801 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
     1861 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
     1921 gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac
     1981 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
     2041 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
     2101 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
     2161 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
     2221 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
     2281 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
     2341 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
     2401 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
     2461 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
     2521 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
     2581 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
     2641 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
     2701 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
     2761 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
     2821 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
     2881 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
     2941 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
     3001 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
     3061 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
     3121 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
     3181 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
     3241 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa
     3301 gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata
     3361 attagagact gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa
     3421 aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat
     3481 aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt
     3541 acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta
     3601 aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat
     3661 agataaactt gctacaacta acactgaaat gattccccat atacctaccg catagtggat
     3721 cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc
     3781 tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca
     3841 agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa
     3901 gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa
     3961 aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct
     4021 aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc
     4081 aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac
     4141 tgtagctaaa ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat
     4201 ttcgttatgt cattaaaaat ctgttctatc aaagttgaat cttctactct aagttttata
     4261 tcttttttta ttccaccatt ttttaaataa ataattctat tgcatgtttt ataaagaaat
     4321 tccggatcat gagtagctat aactactgca cccttttctt ttaagtcttt aataaggtca
     4381 gcaatccttt ccatattaat ataatccaac ccacttgtag gttcatcaaa tagaaataaa
     4441 ttagtttttg acaatgaagc tatagaaacc aataatcttt gtttttctcc accggataaa
     4501 tcaaacggta attgatcttt cattttataa agttccattt tttttaaaat tttttcttgc
     4561 tgatctttac taactatttc attccccagg gctaactcgc tcctcactga ttctgtgaaa
     4621 aattggtaat ccatttcttg cattactaaa aacggcgatg acaaattttt tttattttcg
     4681 attatctttc ctttggtagg gcgaataatc ttcatgagta ttcttagtaa ggttgttttc
     4741 cccacaccgt ttttccctac caaaccaata acatctccaa ctttgacatc aaaatttatg
     4801 tcttttagaa tattttcata agtaatttct tcaactttta atacatctgc acttaccttt
     4861 tcgatatagg gaacttcaat cgaactatta aaaatgtcaa atttccttaa tgcgttattg
     4921 ttttcagttt ttaacaactg aatagttgta gtttttacag ttctattttt aacaaataga
     4981 acttgatcaa ttaattcttt caaatagtaa aaacgatgtt cagctataac aatagtaaat
     5041 cctttatttt ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa
     5101 tttgaagacg gttcatccaa tataattaat ttagagttta gcgataaact acatccaata
     5161 gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa
     5221 agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca
     5281 aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta
     5341 ggatcttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc
     5401 gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt
     5461 ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta
     5521 agactgcctt tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta
     5581 tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa
     5641 gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata
     5701 gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc
     5761 ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag
     5821 aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat
     5881 ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt
     5941 cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga cactaaccaa
     6001 caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg atgtcgcaaa
     6061 aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac
     6121 tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac
     6181 tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac
     6241 cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc
     6301 tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata
     6361 acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttaa
     6421 ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag
     6481 catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga
     6541 gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat
     6601 agtctttaac ttatctagac aacagactgt ctttttcgtc acaaatgatg taattttttc
     6661 aacaatatct tgatatttgg ttccagctga ttggaataaa atcaaatcga atatacgttt
     6721 attctcaaaa atataattaa taaatggatc taattcattg aagttctctc tccaactttt
     6781 ctctatatct atattttcta agtttaagag gtatttggta aactcttcat tatacatatt
     6841 aataagcgca ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa
     6901 atgtttataa aaagcacccg tagttacgcc tgcggtcagg cacagttgcc ttatcttagt
     6961 ttgctgaaat ccttcttttt caaaatggct tagtccactt tctattaact tttggtgagt
     7021 tataatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat taactttatt
     7081 atcttacaca ggacttgaag tttcaatcgt taacgtttat tactgaactc attccttaga
     7141 gctaaactaa tgagaaagag cagaataact gatacaaaaa gcaatcctac aagaattaat
     7201 agactcatct aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat
     7261 atccagctat taggataagt cctatcataa tcactccaat agccattttc ttatcctccc
     7321 taaaaataaa gtcttttact taaaaccttt tttgtaatcg tcaataaatt ttttcccttt
     7381 atcagttaat tgaaatccta tatctaaata gtcctcatat ctaagtttag ccagcatctc
     7441 acttacagat gctggactaa cacttagaac ttttgctaaa tcattatttt ttaatttctt
     7501 ttttcttttt gataattcct gaattgctag taaataaatt agtatagtag tcgttttaat
     7561 ttgtaccata cctaccctcc aaattatttc gaaaggcctt cagcaatttt atccaggttc
     7621 catttcatca tcgcatagta actatcacca tcttgtcctt tttctgcaat tgaatcagta
     7681 aaaatcgttg aatagatagg aatattggta tcttttgata ctgttttcat tggtctatca
     7741 tccacactgc tttctacgaa taaggaggga acttttgttg tgcgtaattt ttcaactaag
     7801 tgttttattt gatctggtgt tccttcttct tcagtgttga tttcccaaat gtaagcagaa
     7861 ggcacattat acgctttcga gaaatattta aagcatcctt cacttgtaac aatcattttt
     7921 ttatcattcg gaattgaagc aaatttagat ttagcttctt tgtctagaga atccaatttt
     7981 tcaatatact tatctagatt ttctttatag aattttttat tatcaggatc tttttccgct
     8041 aactgttttt caatattttt agcgtaaata ataccgtttt ctaaatttaa ccaagcatga
     8101 ggatcttcct tccctttctc actctgaccc tctaagtaaa taacatctat gccatcactt
     8161 gctgcaaaat agtctttgtt ttcctctttg ttcgcatttt taactaattt tgtaaaccaa
     8221 gcatttcctc cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt
     8281 ttttgaacat cttcaggcaa aggttcatat tcgtggggat ctttcccaat aggtacgata
     8341 ctgtgtaaat caattttatc ttttgctata ttttcggtaa tatctgctaa aatcgaatta
     8401 gtaactacta cttttaattt ttcgttcgaa gccactttat ctttgtcttt actatttatt
     8461 gaacatgccc cgaatataaa aagagcaatc aaaatcccca gacttcccag tactgctttc
     8521 cattttctca tcataacgat ctctccttct ttcttaaaaa tttttgtttt ggtgagatta
     8581 gaaaactaat tacaaataag aatgccgctg ttaaaacaat acttgatcct gctgctatgt
     8641 taaaactata accaataaac aatccaagca atgatgaact tgctccgact gttgcagaaa
     8701 taaatatcat agttttcaag ctatttgcat ataaatatgc tgtagctgct ggtgtaatta
     8761 acattgcaac aattaatata gttcccacac tttgcatagc tgttactgct actaaagtta
     8821 atagaaccat caatagataa tgataaaaac tgacattcat ccctattgct tttgccatta
     8881 atggatcaaa agaagtgatt aataattctt taaaaaatag aactactata accaacacta
     8941 aaaccgaaac gccaattgta atccacttat caatatcttg tacagctaaa atatttccaa
     9001 ataaaatatg aaataaatcc gttgagctat ttgcaactcc tataaggata actcctagtg
     9061 ctaaaaaaga actgaaagta ataccaattg ctgtatctcc ttttattaca ctattgcttt
     9121 ttataaaagt aataatgata gaagctaata gaccaaatac aatcgcacca ataaaaaaat
     9181 taatacctaa aatataagaa attgctactc ctggcaaaac agcatgagaa atagcatcac
     9241 ccataagaga cattcctctt aaaataataa aacaacctac tgttccagaa acaatcccta
     9301 taactacaga tgtaattaga gcattctgta aaaagtgaaa gtcatacaga ccattaataa
     9361 aattttgaag catattattc cccctctcca ataaatatag tatctccata ggcttttttt
     9421 aaattttctt cattaaatac agattctaca cttccgtgag caattagatt tcgattcaaa
     9481 agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac aattagcact
     9541 gtttttccct gatgtttcaa tttttttaac gtatccataa ttattcgttc actaactaag
     9601 tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc aagacatctt
     9661 gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg attagaatat
     9721 tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc atttttaatt
     9781 ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt tacttttatt
     9841 ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac cagtttttta
     9901 ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt taacattgct
     9961 ttaagcaatg tggattttcc agcaccattt ggaccaataa tacctgttaa agcaccttct
    10021 tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac attaagcttt
    10081 ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat catatggtat
    10141 acaaaaatta tattttagtc aaccctaaat tttatagttt agattttttt ctgttgctga
    10201 agattagaat gacaaaacta tttgaaacta cgcgtatagt aagtagcttt caaaatatat
    10261 aaaaaaggag cgttctcaaa tgagaacatg ctcctaaaac tactttattg gcgtatggaa
    10321 aaattatact tgaagtggat agcgggttga ataagttgat aagcatcaaa acatttataa
    10381 aaattattca agactatgtc tgttccatat acaaatatga aaatcagtaa actccattta
    10441 acttttttcg tagtgtgtct ttttatacat gtttatcgtt attaatagtg aataggaaaa
    10501 aaactatttt tgatcattga caattaaata caatttttga ataacgatcc ctgctattcg
    10561 agctagaaac tcaagtacgc catgacctga ttagcgacaa atacttagag atttcttttt
    10621 gttctgggaa aacaaaatgc catgacaata gcagcaaata atgatacttc cgtaaggctc
    10681 gccgtaaact ggggagaggt gaaattccta tgtgcttacc caaagcaatt caaaaatttc
    10741 ataactacta ttttaggaga tgattttata gaatccagag tctacctgaa taaaaaacaa
    10801 acctgtgcat ttctaggaat cgataataat actctagata agtggattcg tatatataat
    10861 ttcccagtaa taagagttgg tggtgtagtt cgctttgacg tagaagatat taaagacttt
    10921 atgaatacat ttaaaataac ttgaaatttt cttctcgccg catatactaa aattagtgaa
    10981 atatactgcc ttactttttt taaggagtga ggattttgcc tacaaagctt tcaaatggga
    11041 aatataaaac taatctgaga tatccaaaaa ggtttaaaga aataacagga attgcatcag
    11101 aaaagtatca aaaaacattt ccaaataggc aattggcgat taaagccgaa aatgatatga
    11161 agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa
    11221 aaataacgtt taaaaaattt tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa
    11281 caattagatc aaacaggcct cctagtgata taaccattag taatacaaaa gatatatttc
    11341 ggttgcatat tttacctatg tttggtgaat acgcaatgaa ttatctaaat atcaatactg
    11401 agataatatc agatgaatta accaaaaaat cgaaagaata tgcaaatata aaaatcataa
    11461 aaggatatgt tcgttccatg ttcgatattg cagaaatact aaattatatc gaatttaatc
    11521 gaactactaa aattattcaa agtatcactg cacctaaaaa aaatgcttta gaagaaaaaa
    11581 gaatacaaga aggtaagcaa gcactatcat caaaagaact tactaattgg atagaagcag
    11641 taaatgatga ttttaataat catttactta cttttcatga ttacacactt tttatgatta
    11701 ccctgtatct tggtgataga aaaagtgaga cttacgcttt gcaatggaaa tatattgatt
    11761 ttgaaaaaca aacggtccgt cttaaacata ctttagataa atatcaaaga aaaaagttta
    11821 caaaaggtcg aaaagatacc gtcattcaag taccagaagt tgtaatgaca ttgctaagtg
    11881 aatggaaatc ggtacaagct gatcaattgt tgaaattaaa aattaagcaa acgttagatc
    11941 agtatctctt tacttatact aaaccatctg gagaagtaaa ttgtcctgtt catgccgatt
    12001 acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc tgacttagtt catttatctc
    12061 cacacaaact ccgtcatact tatgcaacta tagctagaca aggtggagca gatatgaatc
    12121 aaatttcaaa tgctttaaca cactcagata tttctacaac aaagatatat gtgaacacac
    12181 cagatatagt tgataaggct gtttttgaag cttttcagag aggattgaat aagtgtgatt
    12241 gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc actttccaaa taattgaaaa
    12301 gtgccagaaa cgttgatata taaaggatat taacgttttg agaattgtga agctttacgt
    12361 gctttcttaa gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca
    12421 gcacgtttaa gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca
    12481 tgacggatag ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca
    12541 tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca
    12601 tgtggaatgt attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact
    12661 agacgtacgc gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc
    12721 aatgaaattc cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct
    12781 gcatgtggat gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa
    12841 gtgtttttag gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca
    12901 cgcaattcgc cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa
    12961 attttgtcag tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg
    13021 aaatcgccag tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat
    13081 gcaacaactg ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta
    13141 cgttctactt cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta
    13201 agttctgatg tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc
    13261 atttaacata ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat
    13321 ttatatgaaa ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat
    13381 gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac cccttatttt gaataaacgg
    13441 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc ataaaacata agaagctgtt
    13501 ttttaaaatt gtttcttgtg actcggagct gaacggcagg cttacaacct cttggtaaac
    13561 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    13621 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttttcgg
    13681 tcaagttcaa caacagtttt tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga
    13741 tcatccagat acgtttttct gtggctgttt tgaattcgat aaagatatct tgtgttggat
    13801 cgtcgccttc ttctccagaa acttcgattc ctttttggta aagatctgct agataacgat
    13861 agcctgcaac aagtgtttcc aaacgttctg gaatagtacg atcccagcgg ccaatttcat
    13921 cagggatttt tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg
    13981 tgatcaagcg ttcggatact tcatctaact gagcgttcaa atcatccatg aactcatcca
    14041 tcattgggtg caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa
    14101 caacagacat ttggcttaga tctgcaacta attgatttaa tacttctttg gtttgttgga
    14161 atttcatctt tcttgcctcc taacattttc ttacacttaa aataatagta gactttcaat
    14221 gaaaagtaca acaaaatcat ttacctgaga acgattttca gttcgctttt ttatccgtaa
    14281 agccaatcat ttttcagaat cagctaattc ttctcgcagt tgagcaaatt gctgataagt
    14341 ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag attttctgat attttttatg
    14401 atcagtttct tgtactccaa cttgctcttt caattctatc aattcctgtg cttcactgat
    14461 actttggatc tttccgatgc ttctccagcc taaaatcaca gctcctaatg cggatgcttc
    14521 tgtgacagta gggaaaacaa gcgggtaacc cagtacatca gccatcatct gacgaaaaat
    14581 cggagattga gcaaacccgc ctgtagcacg gatctctgtg agctgatttt cctggcaaac
    14641 tccttttaaa atccgataaa ggttgagaca gatcccttct agaatcgcct tcatcatttc
    14701 tttttctgta tgttgccgct gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag
    14761 cggtgctcgc tcaccaagta aatagggatg aaaaatcaga ccattggcac ccgctggcac
    14821 aaaagcgata gccgaaagca gtgcatcata tggattttct cctttttctt tagcagattg
    14881 aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac cattgctagt
    14941 cgctcctccc actacccaat gtttctgatc cagaacataa caaaacgtct ccatttctgg
    15001 atggaactga ggctgagtag taataaaacg aagtgctccg ctcgttccta cggtcaaagc
    15061 ggcgatcgta tcaccaaaag cacctaaccc tagattagaa agcggcccgt ctgcccctcc
    15121 caaaacaaat gatatatcat ttggcaaacc tagttctgct gctgtttttg atggtagacc
    15181 tgttacttga taggtggaag gaaagacttg cggcaattgc tctattcgaa tgttcaagta
    15241 gtcaagcact tcttctgccc attggaattc atggatatca aaatatccag ttcctgaagc
    15301 tgttccgtag tcgctgatca gttcgccaaa caatctataa aagatatagt ctttgatacc
    15361 gacaaaataa gcagtttttt tgaataattc aggttggctg ttttgcagcc acttgatttt
    15421 gttcaatggt gtcattggat gaatcggcgt cccagtctgc cgataaaatt ctttgccaag
    15481 cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat
    15541 tcgagataac ggtgttttct cttcatccaa caatatcagg ccatgcatcg cagaggagaa
    15601 ggagacaaat tgaatgattg tgtctgaagg caactgttta attacttctt ttataacggt
    15661 gcagacacct tgaaagatca attcctgatc ttgttctgcc atcccttgtg tttcttggat
    15721 caaagggtag cgaaaataag cggatgctgc ttcttgggct tcatctgtga aagcaactgc
    15781 tttggtttgt gtggttccga tatctaatcc gattgaaacg ttcatttttt cacctcaaat
    15841 tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa aagggggaga
    15901 tggcgtgtta ctttatttta tcattggttg ctgtatcgga tcatttttat gtttaaccgc
    15961 ccagcggatt ccgttaggtc actctattat ttatcctaga tctcattgtg tcaaatgctg
    16021 tcgttcgctt tcttggtatg aattgattcc gattctctcg atcatcgtcc aacgatttcg
    16081 ctgccgctat tgcaggtgtc ggttgccctt ctactatttg ttggcagaag ccttatgtgg
    16141 tggtcttttt gcctggttct ttactttttc ttccgcaaga aatttttcga cgttcatatg
    16201 gatgctttcg gctttgctat tttcgttgat ggatttattt tacttcatgg tccattctca
    16261 cacgctattc tgttcttggg caattttatg gattttttgg ttgcaggccg gcgtcttcca
    16321 atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc
    16381 ctatctcgga gctggcgata ctctgttgat gctttcttgg agtggtggct tgtctctaga
    16441 agagttgctt cagatcttgt ttcttgctag tctgtttgga atatccgttt tttctgttta
    16501 ctttatctgt cataaaaaaa agttggaaaa gctgcctttc attccttttt tgagcagtgc
    16561 gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca
    16621 acctctgata tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt
    16681 cacaacctct ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct
    16741 ggttttacaa cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt
    16801 gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta
    16861 atggagcttg tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct
    16921 tcttggttcg atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg
    16981 ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata
    17041 agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata
    17101 gatttccacc aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc
    17161 tgtatgccct gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc
    17221 agtataaccg gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca
    17281 ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc
    17341 atcatacctt gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac
    17401 tattatatac gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg
    17461 gttaaatcag gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat
    17521 acatgcatgt taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag
    17581 catgcatttg gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt
    17641 tttgcttttg atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt
    17701 ttttctaaaa tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt
    17761 tttttcaagt actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt
    17821 tgaatcgctt gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct
    17881 gagaatttct gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat
    17941 attcctttca gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa
    18001 accagccacg cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat
    18061 agtgctgctt tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg
    18121 cgatgttttt agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag
    18181 gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc
    18241 ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag
    18301 caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga
    18361 ttacgaggtc tgcttgctgt acccgtttat cgaactccag taaatccaat acgtagtcga
    18421 tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag
    18481 cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa tcatgcatgg
    18541 cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg aaaacaaaag
    18601 aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg attttggctt
    18661 taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct agattttctc
    18721 ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga ccttctgcca
    18781 tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg tgtcgaacac
    18841 cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac ctaagtggct
    18901 gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca aaaatcgcag
    18961 tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta gtaacgactg
    19021 ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct tctcctccat
    19081 cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa
    19141 tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc gcaatcagaa
    19201 tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt attggtctcc
    19261 attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt gtttttttgt
    19321 caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt tttcccatgc
    19381 tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac ctgaaaaacc
    19441 aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt catctgatga
    19501 atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat acgcccattc
    19561 catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg atgagtcgtg
    19621 gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag aaaaatactc
    19681 aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga gttcatttac
    19741 ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt tgtaagtggt
    19801 caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct gttctttgtt
    19861 aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa aaagcggatc
    19921 gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt tcgtatatgc
    19981 attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta tctctaaatg
    20041 atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt
    20101 tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt gaacggcata
    20161 agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc
    20221 ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc
    20281 ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg cgccttctaa
    20341 tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat ataaaaagaa
    20401 aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt cgccagttag
    20461 tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct gtaaatggta
    20521 agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg ttatccgatg
    20581 ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg tataactaac
    20641 gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa gagaggtaga
    20701 gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga
    20761 agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt tagctgcaat
    20821 cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa
    20881 tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat
    20941 acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc
    21001 tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca ctttttttcc
    21061 ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat ctagtgcccg
    21121 ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc
    21181 ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta aaggtcggtt
    21241 atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct cctggcttcc
    21301 tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat tgatatactc
    21361 taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt ttctgccgaa
    21421 cgatataaat tttccaccag attctggatg acatatcctt gccagccatc tgcaatcatc
    21481 tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc atgtgggtca
    21541 ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc gaatagttcg
    21601 cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag tttgtcttct
    21661 ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc taattccagt
    21721 gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc gaatttccct
    21781 gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt tactttagga
    21841 aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat caaaggcccg
    21901 cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga ccgacgaagc
    21961 gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt gatcattgtt
    22021 gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt tttatcggct
    22081 gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc gcacatcact
    22141 gaagtattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt gggtgtacag
    22201 acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt atacgcatga
    22261 gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc aaccacagcg
    22321 gccaattcta agacatctga tcttttttga taataagcag catgtacttt ttctgctact
    22381 tgtccagcgc ctatgattgc tactttcttc atttatttct ccctcctttg cttatttact
    22441 tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct gtttccagac
    22501 agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct cctcgcaaac
    22561 gacattcata taccatataa ccttcatact cattttgaag tagcgtttga aaatggcgag
    22621 taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa tggatatgac
    22681 ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca atattcatat
    22741 gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg taatgacgcg
    22801 catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc aaataaataa
    22861 ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg actgctttat
    22921 agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg aacattcccc
    22981 aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc aaaatctcag
    23041 caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc cacccatcgt
    23101 agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt ttgactgtgt
    23161 cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca cagtctttga
    23221 taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt
    23281 tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg ggtgctggtc
    23341 tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat caatgatttc
    23401 ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg
    23461 gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga cgatttttgc
    23521 attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc
    23581 aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta atgcggcagc
    23641 actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt cctttagacg
    23701 aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt tttctgctaa
    23761 ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg cttttttagc
    23821 taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag catcacctgc
    23881 tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt aacatactgc
    23941 atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa caccgtttgt
    24001 aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt gatactctgt
    24061 caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt gaaattcgcc
    24121 aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc attcttctga
    24181 aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg ttcctggttt
    24241 tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta
    24301 atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct tttctttcct
    24361 ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta ataaatcgtt
    24421 cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg gccatcattc
    24481 gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt aaagtaaatt
    24541 tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa accataaagc
    24601 aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc cgaataatga
    24661 tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt
    24721 caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc atcgtgataa
    24781 tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat aatggaacaa
    24841 cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt ttcgagcgag
    24901 ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact actgcagtta
    24961 cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat ggaaaaatct
    25021 cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa atagtttgtg
    25081 tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc aaaaatggaa
    25141 acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt
    25201 tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg caataatgat
    25261 gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg
    25321 atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat ctgtaaacat
    25381 atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt tgattgccgg
    25441 cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag ctgcttcata
    25501 aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat acggatagta
    25561 tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt cgaaccacag
    25621 cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga ttccataact
    25681 attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa cataacttaa
    25741 taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca aaatagctac
    25801 ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc
    25861 taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta atccgataaa
    25921 aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag
    25981 cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa gtcccatggc
    26041 taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct gataatctgc
    26101 caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt tcgtattttt
    26161 ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt ggttcactgc
    26221 ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct tttccccgac
    26281 tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca gctcgccaaa
    26341 agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt tattcactgg
    26401 ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt gagataagaa
    26461 agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag aaatcgtcaa
    26521 tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa aaccgatatt
    26581 ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga ttgccattgg
    26641 tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc ctggcatcgt
    26701 gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg tatcgatcgt
    26761 tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt gagagaacgc
    26821 ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt tattctcttt
    26881 atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc
    26941 agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc taattggtgc
    27001 agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt cataataatt
    27061 atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca gttcatcttt
    27121 atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt taccggaacg
    27181 agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt gcttgatttt
    27241 gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac ttaatcgttc
    27301 ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt tagagctttg
    27361 actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag taacaataat
    27421 ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat tcatacggct
    27481 ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac aggtctaggt
    27541 aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg ataactaaga
    27601 ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag tccagtaagt
    27661 aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc atctgggtca
    27721 attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca atatctgcta
    27781 acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg cctttgtaat
    27841 catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt aacgttacca
    27901 aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct gttggagaac
    27961 ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca tctgggtttt
    28021 tcttgtagtt tactcgggta ccctctttct tcagctgttc cactaattca aggatcgttt
    28081 tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag gctaaaaaat
    28141 taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct ttcaacttac
    28201 ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca
    28261 tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt ggcacatttg
    28321 tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg aataattgga
    28381 tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc cataaaaatc
    28441 ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga
    28501 tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct tcgctaaaag
    28561 tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg
    28621 tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata aaaaaatctt
    28681 tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca gacatatgat
    28741 tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata tcttcccatg
    28801 tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca tcagatgtat
    28861 atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac ttttgataaa
    28921 atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc ccataagtaa
    28981 tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc ttctgctgat
    29041 aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct gagtcgcgat
    29101 aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg gagcgttccc
    29161 aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt aaataatttt
    29221 gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag cttcttaaaa
    29281 aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa
    29341 agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag gtgcatataa
    29401 aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct
    29461 ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt gtacttcaag
    29521 caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca ggaaatggtt
    29581 ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc aatccttttt
    29641 tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc gacttgattc
    29701 tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa
    29761 aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt ctttttgaat
    29821 tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac tacacgaaca
    29881 tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa acactgatca
    29941 agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat gtatccaatg
    30001 atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc cgtaatgaat
    30061 ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat gatttaatga
    30121 agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca ttgagtattt
    30181 acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc gcaggattca
    30241 gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac attccgacaa
    30301 atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac caataccctg
    30361 aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc caacaacaag
    30421 aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt tactctgttc
    30481 ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat ttttcttatt
    30541 ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact attttttcca
    30601 cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt tcgttttttt
    30661 aaacacattt acccttattt caatccatgt tatttgggga tacttatctc taaaacataa
    30721 atagaataac agacatgtct actggttttg ttttattcga ggaattattc aataatagct
    30781 agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca ttcaaaaaat
    30841 gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc gataccttaa
    30901 ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt agacaaatta
    30961 ttttagac
//
LOCUS       DAYYCM010000025        30907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000025 DAYYCM010000000
VERSION     DAYYCM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.026"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHAY1_001258"
     CDS             complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHAY1_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8593264.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1699..1827)
                     /locus_tag="ACHAY1_001259"
     CDS             complement(1699..1827)
                     /locus_tag="ACHAY1_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593265.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2172..2315)
                     /locus_tag="ACHAY1_001260"
     CDS             complement(2172..2315)
                     /locus_tag="ACHAY1_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8593266.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="ACHAY1_001261"
     CDS             complement(2538..3017)
                     /locus_tag="ACHAY1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8593267.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="ACHAY1_001262"
     CDS             complement(3090..3374)
                     /locus_tag="ACHAY1_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593268.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="ACHAY1_001263"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAY1_001263"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="ACHAY1_001264"
     CDS             complement(3718..5004)
                     /locus_tag="ACHAY1_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8593269.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="ACHAY1_001265"
     CDS             5142..5813
                     /locus_tag="ACHAY1_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8593270.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="ACHAY1_001266"
     CDS             5779..6267
                     /locus_tag="ACHAY1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8593271.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="ACHAY1_001267"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="ACHAY1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8593272.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="ACHAY1_001268"
     CDS             7228..7992
                     /locus_tag="ACHAY1_001268"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8593273.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="ACHAY1_001269"
     CDS             8225..10438
                     /locus_tag="ACHAY1_001269"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8593274.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /gene="dtd"
                     /locus_tag="ACHAY1_001270"
     CDS             10449..10895
                     /gene="dtd"
                     /locus_tag="ACHAY1_001270"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8593275.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="ACHAY1_001271"
     CDS             11066..12325
                     /locus_tag="ACHAY1_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8593276.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="ACHAY1_001272"
     CDS             complement(12414..13361)
                     /locus_tag="ACHAY1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8593277.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /gene="hisS"
                     /locus_tag="ACHAY1_001273"
     CDS             13755..15047
                     /gene="hisS"
                     /locus_tag="ACHAY1_001273"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8593278.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="ACHAY1_001274"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="ACHAY1_001274"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8593279.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="ACHAY1_001275"
     CDS             16940..17815
                     /locus_tag="ACHAY1_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8593280.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18081..19964
                     /locus_tag="ACHAY1_001276"
     CDS             18081..19964
                     /locus_tag="ACHAY1_001276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8593281.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20039..20323)
                     /locus_tag="ACHAY1_001277"
     CDS             complement(20039..20323)
                     /locus_tag="ACHAY1_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8593282.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20313..21047)
                     /locus_tag="ACHAY1_001278"
     CDS             complement(20313..21047)
                     /locus_tag="ACHAY1_001278"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8593283.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21176..21799
                     /locus_tag="ACHAY1_001279"
     CDS             21176..21799
                     /locus_tag="ACHAY1_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8593284.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21991..23112
                     /locus_tag="ACHAY1_001280"
     CDS             21991..23112
                     /locus_tag="ACHAY1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8593285.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23119..26247
                     /locus_tag="ACHAY1_001281"
     CDS             23119..26247
                     /locus_tag="ACHAY1_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8593286.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26266..29460
                     /locus_tag="ACHAY1_001282"
     CDS             26266..29460
                     /locus_tag="ACHAY1_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8593287.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29606..30313)
                     /locus_tag="ACHAY1_001283"
     CDS             complement(29606..30313)
                     /locus_tag="ACHAY1_001283"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8593288.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10121 a         5590 c         6531 g         8665 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
    18001 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
    18061 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
    18121 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
    18181 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
    18241 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
    18301 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
    18361 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
    18421 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
    18481 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
    18541 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
    18601 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
    18661 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
    18721 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
    18781 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
    18841 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
    18901 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
    18961 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
    19021 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
    19081 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
    19141 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
    19201 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
    19261 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
    19321 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
    19381 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
    19441 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
    19501 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
    19561 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
    19621 ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
    19681 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
    19741 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
    19801 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
    19861 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
    19921 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
    19981 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
    20041 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
    20101 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
    20161 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
    20221 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
    20281 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
    20341 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
    20401 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
    20461 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
    20521 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
    20581 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
    20641 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
    20701 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
    20761 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
    20821 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
    20881 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
    20941 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
    21001 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
    21061 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
    21121 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
    21181 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
    21241 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
    21301 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
    21361 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
    21421 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
    21481 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    21541 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    21601 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    21661 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    21721 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    21781 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    21841 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    21901 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    21961 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    22021 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    22081 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    22141 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    22201 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    22261 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    22321 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    22381 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    22441 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    22501 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    22561 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    22621 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    22681 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    22741 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    22801 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    22861 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    22921 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    22981 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    23041 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    23101 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    23161 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    23221 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    23281 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    23341 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    23401 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    23461 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    23521 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    23581 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    23641 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    23701 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    23761 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    23821 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    23881 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    23941 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    24001 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    24061 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    24121 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    24181 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    24241 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    24301 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    24361 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    24421 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    24481 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    24541 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    24601 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    24661 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    24721 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    24781 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    24841 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    24901 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    24961 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    25021 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    25081 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    25141 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    25201 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    25261 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    25321 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    25381 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    25441 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    25501 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    25561 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    25621 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    25681 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    25741 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    25801 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    25861 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    25921 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    25981 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    26041 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    26101 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    26161 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    26221 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    26281 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    26341 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    26401 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    26461 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    26521 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    26581 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    26641 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    26701 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    26761 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    26821 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    26881 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    26941 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    27001 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    27061 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    27121 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    27181 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    27241 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    27301 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    27361 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    27421 aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag
    27481 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    27541 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    27601 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    27661 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    27721 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    27781 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    27841 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    27901 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    27961 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    28021 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    28081 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    28141 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    28201 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    28261 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    28321 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    28381 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    28441 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    28501 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    28561 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    28621 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    28681 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    28741 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    28801 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    28861 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    28921 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    28981 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    29041 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    29101 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    29161 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    29221 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    29281 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    29341 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    29401 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    29461 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    29521 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    29581 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    29641 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    29701 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    29761 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa
    29821 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    29881 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    29941 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    30001 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    30061 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    30121 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    30181 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    30241 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    30301 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    30361 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    30421 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    30481 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    30541 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata
    30601 actattcaaa cataaaataa cgttatttga tttatttgtt tatagtaaat tttatttagt
    30661 aatcattgct ttaaaatact acgcttatat aacagagaaa ataagcgtta taataaagaa
    30721 cagtattatt taaaatgaca gaaaacatta ctattaaaga aataaaaaac ataaaaaatc
    30781 ttaagaaatc ggtttcattt agcttttgag ataaaataat agtacagttt tatacgctgt
    30841 ttcatatgtt tagaaaattt gaattagatt tgaaaactct aatttttaaa tgataaaaaa
    30901 agcgaga
//
LOCUS       DAYYCM010000026        30899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000026 DAYYCM010000000
VERSION     DAYYCM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.027"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHAY1_001284"
     CDS             complement(296..1024)
                     /locus_tag="ACHAY1_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593289.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHAY1_001285"
     CDS             complement(1002..2576)
                     /locus_tag="ACHAY1_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8593290.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHAY1_001286"
     CDS             2805..3743
                     /locus_tag="ACHAY1_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593291.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHAY1_001287"
     CDS             3755..4666
                     /locus_tag="ACHAY1_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593292.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHAY1_001288"
     CDS             4690..6162
                     /locus_tag="ACHAY1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8593293.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHAY1_001289"
     CDS             6213..6824
                     /locus_tag="ACHAY1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8593294.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHAY1_001290"
     CDS             6854..8767
                     /locus_tag="ACHAY1_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8593295.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHAY1_001291"
     CDS             8895..11876
                     /locus_tag="ACHAY1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8593296.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHAY1_001292"
     CDS             11873..13534
                     /locus_tag="ACHAY1_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8593297.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHAY1_001293"
     CDS             13574..14506
                     /locus_tag="ACHAY1_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8593298.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHAY1_001294"
     CDS             complement(14898..16445)
                     /locus_tag="ACHAY1_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8593299.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHAY1_001295"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHAY1_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8593300.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACHAY1_001296"
     CDS             complement(16890..17084)
                     /locus_tag="ACHAY1_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593301.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHAY1_001297"
     CDS             complement(17310..18512)
                     /locus_tag="ACHAY1_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593302.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHAY1_001298"
     CDS             complement(18515..18709)
                     /locus_tag="ACHAY1_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8593303.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHAY1_001299"
     CDS             complement(18756..19604)
                     /locus_tag="ACHAY1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8593304.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHAY1_001300"
     CDS             complement(19809..20159)
                     /locus_tag="ACHAY1_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593305.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHAY1_001301"
     CDS             20337..21200
                     /locus_tag="ACHAY1_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8593306.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACHAY1_001302"
     CDS             21824..22816
                     /locus_tag="ACHAY1_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593307.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACHAY1_001303"
     CDS             22832..23716
                     /locus_tag="ACHAY1_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593308.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACHAY1_001304"
     CDS             23765..25372
                     /locus_tag="ACHAY1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593309.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACHAY1_001305"
     CDS             25373..26857
                     /locus_tag="ACHAY1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8593310.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACHAY1_001306"
     CDS             27034..28026
                     /locus_tag="ACHAY1_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593311.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACHAY1_001307"
     CDS             28078..28947
                     /locus_tag="ACHAY1_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8593312.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACHAY1_001308"
     CDS             complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACHAY1_001308"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8593313.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         9690 a         5145 c         6234 g         9830 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataatttt
//
LOCUS       DAYYCM010000027        30789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000027 DAYYCM010000000
VERSION     DAYYCM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.028"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..299)
                     /locus_tag="ACHAY1_001309"
     CDS             complement(135..299)
                     /locus_tag="ACHAY1_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593314.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            594..1922
                     /locus_tag="ACHAY1_001310"
     CDS             594..1922
                     /locus_tag="ACHAY1_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8593315.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2124..2957
                     /locus_tag="ACHAY1_001311"
     CDS             2124..2957
                     /locus_tag="ACHAY1_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8593316.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2973..3710
                     /locus_tag="ACHAY1_001312"
     CDS             2973..3710
                     /locus_tag="ACHAY1_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8593317.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3865..4833)
                     /locus_tag="ACHAY1_001313"
     CDS             complement(3865..4833)
                     /locus_tag="ACHAY1_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8593318.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5065..5898
                     /locus_tag="ACHAY1_001314"
     CDS             5065..5898
                     /locus_tag="ACHAY1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8593319.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5932..6771
                     /locus_tag="ACHAY1_001315"
     CDS             5932..6771
                     /locus_tag="ACHAY1_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8593320.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6797..7357
                     /locus_tag="ACHAY1_001316"
     CDS             6797..7357
                     /locus_tag="ACHAY1_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8593321.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7556..9277
                     /locus_tag="ACHAY1_001317"
     CDS             7556..9277
                     /locus_tag="ACHAY1_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8593322.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9442..10584
                     /locus_tag="ACHAY1_001318"
     CDS             9442..10584
                     /locus_tag="ACHAY1_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8593323.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10600..11811
                     /gene="thiI"
                     /locus_tag="ACHAY1_001319"
     CDS             10600..11811
                     /gene="thiI"
                     /locus_tag="ACHAY1_001319"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8593324.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12384..13031
                     /locus_tag="ACHAY1_001320"
     CDS             12384..13031
                     /locus_tag="ACHAY1_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8593325.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13160..13376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13424..16069
                     /locus_tag="ACHAY1_001321"
     CDS             13424..16069
                     /locus_tag="ACHAY1_001321"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8593326.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16379..17692
                     /locus_tag="ACHAY1_001322"
     CDS             16379..17692
                     /locus_tag="ACHAY1_001322"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8593327.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17682..18335
                     /locus_tag="ACHAY1_001323"
     CDS             17682..18335
                     /locus_tag="ACHAY1_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8593328.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18390..19082
                     /gene="radC"
                     /locus_tag="ACHAY1_001324"
     CDS             18390..19082
                     /gene="radC"
                     /locus_tag="ACHAY1_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8593329.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19232..19432
                     /locus_tag="ACHAY1_001325"
     CDS             19232..19432
                     /locus_tag="ACHAY1_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8593330.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19686..21365)
                     /locus_tag="ACHAY1_001326"
     CDS             complement(19686..21365)
                     /locus_tag="ACHAY1_001326"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8593331.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILSGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21367..21579)
                     /locus_tag="ACHAY1_001327"
     CDS             complement(21367..21579)
                     /locus_tag="ACHAY1_001327"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8593332.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21972..23702
                     /gene="efrA"
                     /locus_tag="ACHAY1_001328"
     CDS             21972..23702
                     /gene="efrA"
                     /locus_tag="ACHAY1_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8593333.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23699..25459
                     /gene="efrB"
                     /locus_tag="ACHAY1_001329"
     CDS             23699..25459
                     /gene="efrB"
                     /locus_tag="ACHAY1_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8593334.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25554..25751
                     /locus_tag="ACHAY1_001330"
     CDS             25554..25751
                     /locus_tag="ACHAY1_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593335.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25904..26389
                     /locus_tag="ACHAY1_001331"
     CDS             25904..26389
                     /locus_tag="ACHAY1_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8593336.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26493..27086)
                     /gene="clpP"
                     /locus_tag="ACHAY1_001332"
     CDS             complement(26493..27086)
                     /gene="clpP"
                     /locus_tag="ACHAY1_001332"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8593337.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27266..27793
                     /locus_tag="ACHAY1_001333"
     CDS             27266..27793
                     /locus_tag="ACHAY1_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593338.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27884..28621
                     /locus_tag="ACHAY1_001334"
     CDS             27884..28621
                     /locus_tag="ACHAY1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8593339.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28625..29542
                     /gene="miaA"
                     /locus_tag="ACHAY1_001335"
     CDS             28625..29542
                     /gene="miaA"
                     /locus_tag="ACHAY1_001335"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8593340.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29544..30773
                     /gene="hflX"
                     /locus_tag="ACHAY1_001336"
     CDS             29544..30773
                     /gene="hflX"
                     /locus_tag="ACHAY1_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8593341.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10170 a         5265 c         6568 g         8786 t
ORIGIN      
        1 ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc ttttattatt
       61 atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga aagagtagag
      121 ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata aatcagcatt
      181 cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc aaagttatct
      241 gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag gtttttcatt
      301 ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat tttgaaagct
      361 atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat tttcgttatc
      421 tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat ttttcttcat
      481 gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct
      541 aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag
      601 ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc
      661 aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag
      721 cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc
      781 aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc
      841 tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata
      901 aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg
      961 atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata
     1021 tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg
     1081 cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg
     1141 cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga
     1201 aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga acacctccaa
     1261 aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga
     1321 caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga
     1381 tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa
     1441 atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat
     1501 tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat
     1561 cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg
     1621 atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc
     1681 atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg
     1741 aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga
     1801 tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa
     1861 atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat
     1921 agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct
     1981 ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata
     2041 aaggataagg attggaaata atccttttat cattaaaaat atgatgacga agtagaataa
     2101 aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa
     2161 atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg
     2221 aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag
     2281 aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag
     2341 gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc
     2401 aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt
     2461 ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt
     2521 ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc
     2581 gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg
     2641 ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt
     2701 tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg
     2761 aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt
     2821 atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat
     2881 attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat
     2941 tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg
     3001 aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag
     3061 tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca
     3121 atagctttgg acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta
     3181 gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt
     3241 atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt
     3301 tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc
     3361 cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt
     3421 cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta
     3481 ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc
     3541 atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg
     3601 aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccgaa
     3661 tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa aaaagaggtt
     3721 gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg tttctcaaat
     3781 ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc tctcttttta
     3841 agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt cttcacccga
     3901 caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat taatactgtt
     3961 acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat cataaacagg
     4021 ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt ggacaagaag
     4081 cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag cttcgattac
     4141 caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca tcccagcata
     4201 tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct ttgtttctaa
     4261 aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac ccaatgtagg
     4321 tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg cagaatggat
     4381 ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac ttgcgacacg
     4441 aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg aacgcattgc
     4501 cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa aagcagtttc
     4561 ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg cataagcgga
     4621 caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg agggaagatt
     4681 gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg catctaataa
     4741 tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca tggcgatcgt
     4801 tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac gaataatttt
     4861 gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca gtgaagaata
     4921 acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa gactcaacct
     4981 tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag cagccaagga
     5041 aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt taaagaacat
     5101 gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt tctaccctta
     5161 gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga gaattggata
     5221 agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat cagcggtcaa
     5281 ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt tttagatgtt
     5341 atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt cccctcatca
     5401 ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg gtttgagggt
     5461 tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt tcttgaacgt
     5521 acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga gccacagttg
     5581 ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc tctttttcga
     5641 ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg taaaaaaaga
     5701 acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt caaagtagtt
     5761 gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg tgatgcggct
     5821 gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact caatccaacc
     5881 caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc tatgatcaaa
     5941 gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc gttggaatca
     6001 acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt agcaacgggg
     6061 cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat gtatgtcgtg
     6121 tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc ttttgaacaa
     6181 aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat cgtttttggt
     6241 gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat ttttatcaag
     6301 cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa aatcctccag
     6361 gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt aaaagaacca
     6421 atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga acatcgcttt
     6481 cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc aaaaggtggt
     6541 tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat ggaagaaatt
     6601 atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg aattggagtg
     6661 gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac tcaatcaaat
     6721 gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta gtcaatgaaa
     6781 gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa cattccacaa
     6841 aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg aagctcatac
     6901 acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt cacctaataa
     6961 agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag cagaagaaaa
     7021 aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag atgcactgac
     7081 agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc ctgatcgaat
     7141 attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa aacctcatta
     7201 tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat atgctccaga
     7261 acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta agaagaaatc
     7321 cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact atttttgtta
     7381 tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca tgaaaatagt
     7441 atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg atttatgcca
     7501 agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac cataaatggg
     7561 aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg tcgtcttttt
     7621 ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag aaaaacgcaa
     7681 ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa aaatgcatct
     7741 ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg acatatctac
     7801 ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca atgaaagttt
     7861 tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg accaaatgga
     7921 gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg aagaacgcaa
     7981 ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa aagtcagaga
     8041 agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca aagtgatcga
     8101 aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg aagcccgcga
     8161 ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga aaaaaattcc
     8221 accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa tcagtgatac
     8281 ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg ctgaagatat
     8341 cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa cagaagttga
     8401 aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat atgatatctt
     8461 agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga ttgcagagta
     8521 tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc acacatctca
     8581 aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa ccgaagtgga
     8641 agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg aaattcctta
     8701 ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata tcgaaaagca
     8761 acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag aagcgcaaga
     8821 aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag aaaaacaacg
     8881 tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg accgtattga
     8941 agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca atcgtttagt
     9001 tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt tagtagatgc
     9061 agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata cacacgaaga
     9121 agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc actatcaaga
     9181 agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt tcaaacgcat
     9241 cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta gtaaaccagc
     9301 aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga aaattgaaac
     9361 aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat agaaaagaat
     9421 ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac cccaatcttt
     9481 ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa tccatcaagc
     9541 ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa gcagatcgct
     9601 gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac agaaggagat
     9661 aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca tatgatcatt
     9721 tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga attaggaatc
     9781 gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga attgaaagaa
     9841 ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga agtaggatcg
     9901 attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat tcatttccat
     9961 gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac agaaagagtt
    10021 gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg tttcatttat
    10081 tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga aagaaatcag
    10141 cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt acgtttatca
    10201 ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc tctaatcaat
    10261 gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta tgcaccgcat
    10321 attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc cttagaagaa
    10381 aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat ggcaagcagt
    10441 acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg aatcagctta
    10501 gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa gcagctttat
    10561 aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag tgaaattatg
    10621 gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat catgcaacta
    10681 gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca tgctgatcgg
    10741 gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc aaaactagca
    10801 aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga tgtcgaagtt
    10861 ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga aacatttaaa
    10921 attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt gaatcaaaca
    10981 ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg tcaaccagat
    11041 attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga aacgatcaag
    11101 ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct ttctgggggg
    11161 attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat tgaagctgtt
    11221 cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa agatctaaca
    11281 gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt taccgagatt
    11341 caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac tcgtcgaatg
    11401 atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat tttgaatggt
    11461 gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat caatgaagta
    11521 acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat cattgaagta
    11581 gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg ctgtacgatt
    11641 tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca atatgaagag
    11701 cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat ggaagagata
    11761 caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata agaggttatg
    11821 aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct ccatgtattt
    11881 taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc aagaagttat
    11941 ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag atcaagctac
    12001 agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg tgacagaagt
    12061 gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt tttgtgaaat
    12121 ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg ttgcgaaaaa
    12181 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttacgtt
    12241 tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg tctcacaccc
    12301 ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact aaatatgtga
    12361 aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca aaagcaactg
    12421 cgaaaagatt acctttatat ttacgctact tgaagttgct ggatgactca ggtataagta
    12481 gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg atccgcagag
    12541 acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc ccttttctta
    12601 tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca ttagtaggat
    12661 atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa aatctaaata
    12721 tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat ggagaaatga
    12781 tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca gtagcaatct
    12841 ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa ggaaatgtaa
    12901 ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc aatgttcaat
    12961 acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat tactcagaag
    13021 ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt cgtaattctt
    13081 tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt gactaaaaaa
    13141 aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc acattcattc
    13201 ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg tagcttggga
    13261 gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca cttgagaggc
    13321 aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc ctagaatacg
    13381 aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg aagaaaataa
    13441 cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc attggttaga
    13501 acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa tcgtaatccc
    13561 gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga ccttgcaaga
    13621 tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc ctggtatgga
    13681 ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc aagggatttc
    13741 tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga aagaagagta
    13801 tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt acagtaggga
    13861 acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg ttaccttata
    13921 cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc aagcacgaac
    13981 agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt atcatatgag
    14041 ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc cagaaacgat
    14101 gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag cgctcatcgg
    14161 caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag atgaatatgt
    14221 agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc caaacgactt
    14281 tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg atggtacgat
    14341 gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta aagcaatcgt
    14401 gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc acagtgtggg
    14461 tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat ggtttgtaaa
    14521 aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag cagtggaatt
    14581 cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc atgattgggt
    14641 aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca aagaaacagg
    14701 tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac aagatcctga
    14761 tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg gctggccaga
    14821 tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa ccggttatga
    14881 catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta ctggcgaacg
    14941 tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc ggaagatgag
    15001 taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg gagcagatgc
    15061 tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct tcagctatga
    15121 aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac gtttcgttat
    15181 catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa aatcagtggc
    15241 tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg agctatttga
    15301 tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg atgatttttg
    15361 tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg tttcaaaaca
    15421 aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat tgcatccaat
    15481 catgccattc gttacagaag aaatatggga acatattcct catcagggaa attctctagt
    15541 agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt ctaaagggat
    15601 ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag tgaatacacc
    15661 attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg atcaattttt
    15721 agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga cgatatcaag
    15781 tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag aaatcttctt
    15841 gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa aagaactgga
    15901 aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac gttttgtttc
    15961 taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt atttagagaa
    16021 gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag aatagaaaaa
    16081 gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc gttacaggaa
    16141 agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa attttgtcgc
    16201 atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat catgtatcaa
    16261 gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc tttgtcatac
    16321 ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg aattgttgat
    16381 gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt cacgtcctgg
    16441 ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa aagtaaaaac
    16501 gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga gaagcctatt
    16561 agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa cgttcaatga
    16621 acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc ttgtaaaaaa
    16681 atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta ttactgaatt
    16741 cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg acgtagcgat
    16801 tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac caatgctgac
    16861 gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa ttgaagaaat
    16921 cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag gaaatattgc
    16981 cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac cgctttatgt
    17041 atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg gagaagtatt
    17101 tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt tagggcaaca
    17161 tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc aattggaaaa
    17221 gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat ggccaggaag
    17281 aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata atacgcatgc
    17341 aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa tcaacatttt
    17401 gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc agcaagtacc
    17461 aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat taagtgaagt
    17521 atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg gattagcaga
    17581 tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac tttatttcat
    17641 ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat aatgcagtga
    17701 tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa gccacacaag
    17761 tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa ttcttaggtg
    17821 tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt ggcaaaacaa
    17881 gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt tcgcaaggag
    17941 cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa catcagattc
    18001 ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg aagaagaaca
    18061 atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga gcaaaaccag
    18121 acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa gaaacattga
    18181 ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc ccggtagtca
    18241 tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct ggggttgttt
    18301 cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt cgtatcttat
    18361 tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa gaagtaccta
    18421 ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta tcggatcagg
    18481 agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg tcgattgctg
    18541 gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact ttgcatgagt
    18601 tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc ttgatcgaat
    18661 taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt accagctacg
    18721 aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat ttggtttgta
    18781 tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc ggttcgctga
    18841 atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat tgtgcagcaa
    18901 ggatcgtttt agcgcataat catccttctg gtaatgttat tccatcgcag caggatatga
    18961 attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta ctggaccact
    19021 tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg gaagagaagt
    19081 aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa ttttgtttgg
    19141 gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt acaatttaaa
    19201 ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa aatggttcaa
    19261 cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat tcgttcactt
    19321 ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag tgactttcga
    19381 cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt aattttatct
    19441 gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag taacaaaaat
    19501 acctgccaca agagacaatt tctcttgtgg caggtatttt tttgtatgaa aaagtaaata
    19561 aatttaagag gctatgaaag agccaccttg tatcaaggaa taagaacaaa caaaaatggc
    19621 ttctcatgtt tttcatttgt tttctcttat attcaggaca aggtgctgat ttcataacct
    19681 cttatttatt cttcatctgg tgtcattacc attggcaaaa tcattggatg tctttctgta
    19741 cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt ctttcaattt atattctgaa
    19801 cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata agattcgctg agcttcattg
    19861 atcatttctc ctgattctcg catgtagaca aatccacgag agagaatatc tggaccagac
    19921 aagatttctt ttttcttgat atccactgtc gcgacagcta atacaagacc ttcttctgaa
    19981 agcaggtgac ggtcacgtaa aacgacatta ccaatatcac cgatcccgct accatcgata
    20041 tacacatctc cagccacaaa atgcccagca ggacgcgcag aatcgctagt taacgctaaa
    20101 acatcgccat tttccatgat gaagcagttt tctgcaggaa cacctacatc ttgtgctaga
    20161 gaagcatgta ctttcaacat gcggaattct ccgtggacag gaataaaata ttttggcttc
    20221 atcaaacgaa gcattaattt ttgttcttct tgtccaccgt gaccggaagt atggatgtta
    20281 ttgatttttc cgtgaatcac ttctgcacca gcttcagaca gcaaattgat caaatgattg
    20341 acacttgttg tattaccagg aataggtgaa cttgaaaaga tcactgtatc gtctggctgg
    20401 atttggattt gacggtgagt accattcgcg atgcggctca acgcggccat cgcttctcct
    20461 tgagaacctg tacataggat catcgtttca ttggcaggca agcgattgat ttcgtgtgca
    20521 tctacaaatg tgccgtctgg aactttgata tatcctaaac gttgaccgtt gacgatggca
    20581 ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag ttgccacagc ggcatctgct
    20641 gcttgctgca aacggaagat gttagaagca aagctggcaa aaatgatacg tccttcgatt
    20701 tttgaaaaga tcttcaaaat agaatgccca atcgtttttt ctgatttagt aaatgttgga
    20761 atctcagcat ttgtactatc agataaaagg cacaagactc cttcttcccc gatacgagcc
    20821 attttatgca agtttgctgg ttcgccaaca ggagtgaaat cgaatttgaa gtctccagtc
    20881 gctacgatat ttccagaagg tgttttcacc acgatcccta atgcatctgg gatactatgg
    20941 gtcgttctga aaaagctgac agaagtcttt ctaaaacgga tgatcgtatc ttcattgatc
    21001 tcatggagct ctgcatctcg aagcaagcca tgctcatcta atttgtttgt gatcaacgct
    21061 aatgcgagag gaccagcata gataggaata tttgcttgtt tcagcaagta gggtattccg
    21121 ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt ttactttgtg taagttttgt
    21181 atgatgtagc tgtagtcggg aatgacatag tcaattccaa gtaaatcgtc ttccgggaat
    21241 ttgatcccgg catcaatcaa aatgatttca tcttggaatt gtaccccata cgtatttttc
    21301 ccgatttcac ctaatccacc aatagcaaag acgcctgtct cgttattttt gatggaaagt
    21361 ttcatattaa aactccacta atgagaaatc agcattttct ttttcgtact ctagatgact
    21421 tccttctaga agttgaacga attcaatatt gtatggggta ttttcttcca cacgttggcg
    21481 agcgtctaca tcgctttttg cctcaatgta aagagatttt gtttcttccc gtttagggtt
    21541 gcgtactttt gtttcttgat aataaacttt atagatcatt gttttgtctc ctttatctgc
    21601 gtaaagcagt gatttagcta gttgattttt ttagacaaca caaaacgagg ggacaagcca
    21661 cctggttttt catatgcttt gactagaata ctggattcga gtcaaagaga caagcctcgt
    21721 ttttgcttat ctgttcggat attttttcaa ccgtaccttc tgtataaaaa atatacaaca
    21781 attaattgtt attctagcat aaaacagcaa ataagtatag aaatcaacta gattgaaaga
    21841 aaaaatcaat gtttatcatc ccgctataat cataatgact tttttatgag agaagtgttc
    21901 tattaatagg ctcgttgtgc tatcattttt agtaatgaaa atgtaacaaa agattaaaag
    21961 gagatgagaa agtgaaacta atgtggcatt ataccatgcg ttataagaaa ttcttgtttt
    22021 tcaatttcat atgcgtattt ggttttattt tgattgaatt aggacttcct acgattttag
    22081 cacgtatgat cgatgtagga atccgcaatg atgattttga ttatgtgaag caacaaggga
    22141 tatttatgat cgttattact gtaatcggta ttattttgaa tatcttgtta ggttattttg
    22201 gttcgaggat tacgactaat atcgtagcag atattcgaga tgatttgttc gaaaagatcc
    22261 aaagttattc gcatcaggaa tacgaaaatc taggcgtatc ttcattgatc actcgtacaa
    22321 caaacgatgc ttatcagatc atgttgttcc tgcaaaatat tttacgtatc ggttttatgg
    22381 caccaatgat gttcattgtc agtttgtaca tggtcatgcg tacaagtgtg acactcagtt
    22441 tgtatgtagt aggtgcatta cctttactat tattggcagt cgtagcgatt gctaaggtgt
    22501 ctgaaccgtt atctaaaaag caacaaaaaa acttagataa aatcaacagt atcttgcggg
    22561 aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa tgaaaagttt gaagaaaaac
    22621 gttttgctgt agtcaatgag aattatgctt ctagttcaaa aagcttattc cgtttgatgg
    22681 ctgttgcaca accgggtttt tatttcttat ttaatatcgt aatggtattg atcatctgga
    22741 gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg agatttgatt gcgtttatcg
    22801 agtatatatt ccatgccttg ttctcattca tgttatttgc tagtgtgttc atgatgtatc
    22861 caagagcagc tgtttcagct ggacgtatcc aagaagccct agatgccaaa ccttcgattg
    22921 tagaagatcc aaatggtata tctgatacaa aaactaaagg gtatatcgaa tttcaaaatg
    22981 taacatttgc ttatccaggc cattcgcaag agccagtgat acgaaatgtc agttttacag
    23041 cctctccagg agagaccgtt gcttttattg gaagtaccgg tagcggaaaa tctacgttga
    23101 tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa gatcttgatc gatggagtag
    23161 acgtgagaga ttatcggcta agcaagttgc gagataagat tgggtttatt ccgcaaaaag
    23221 cgctgctatt tactggaacg attgcagata atttgcgata cgggaaagaa gatgctacgc
    23281 aagaagaaat ggaacgtgca gctgagattg ctcaagcttc agaattcatt tctcgaaaac
    23341 cagatggatt taatgaacat ttatcagaag gtggttccaa tttttcaggt ggacaaaaac
    23401 aacgattggc aatagccaga gccatcatac gccgtccgga aatctatatt tttgatgaca
    23461 gcttttctgc attagattat caaacggatg cgaagttacg tgctcgtctg aaaaaagaaa
    23521 cgacagaatc tactgtattg atcgtagcac aacgtgtggg aacgatcatg catgcagata
    23581 agatcatcgt attaaatgaa ggagaagtcg tcggtatggg gacgcatcgg gagttgttgg
    23641 aaaaatgccc gatttattat gatattgccg cttctcaatt atcaaaggag gaattagcat
    23701 gaaaaaaacc ttttcttcct taaaacgttt agctcgatat atcagaccgt atcgtgagac
    23761 ttttctttta gtgatcctat ttacagttct gacagtggct ttcaacgtgg ctttgccata
    23821 tgttacagga cttcctacta cagaaatcag ccgtaatatt gccaatggcg aatcaatcaa
    23881 tttttcttat atattcaaat gtctcatttg gatcacagtt gttggaatag ggtattgtat
    23941 ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt caaagtgcca tgcatgattt
    24001 gagaagagac atagaagaaa aaatcaatcg tcttccggtt tcatatttcg ataaaaacca
    24061 gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat gcagtcagcg gtgctttaca
    24121 acaagctttt attggtatcg taaatgcgat attgggaatt acgttggctg cagcaatgat
    24181 gttttatatc cagccgatga tggcgctgat ctctatgatc atgattccaa catcgatctg
    24241 gatctcaaaa cgagtgatca atgcttcgca gaaatatttt caaagcatgc agaattctct
    24301 tggtgagcta aacggctacg tccaagaaaa tatgacaggt tttagtgtat tgaaagtata
    24361 tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt aatcacagct tgaaatactt
    24421 tgggtttcgt gcagcattca tttctggctt gatgatgcct ctagttcaat taactgccta
    24481 tgctacttat attggtatgg ctgttctagg aagcttttat gttattactg gggcgatcgt
    24541 tgtcggtcaa ctacaagcat tcatccaata tatttggcaa atcagtcagc caatggggaa
    24601 cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg gcaacgaaac gtgtattcga
    24661 gattttagat gagccagaag aaaaagaaaa caaagtagat gcttcactgc cagaaacgat
    24721 tttaggagat gtgacattcg atcatgtcga ttttgcatat gatccgaaga aaccattaat
    24781 ccaagatttg aattttgaag tgaaagctgg tcaaaccgta gctgtggtcg gtccgaccgg
    24841 tgccggcaag acgacattga tcaatctatt gatgcgtttt tatgacgtag acaaaggagc
    24901 aataaggatt gatggaatcg acaccaaatc aatgagtcgc agtgatgtgc gttcgctatt
    24961 tggtatggtc ttgcaagatg catggcttta tcaaggaacg attgcagata atatccgctt
    25021 cggaaagctt gatgcgacag attatgaagt agttgatgct gcgaaaacag caaacgtaga
    25081 tcattttatc cgaacgatgc cagatggata tgatatggcc atcaattctg agggagacaa
    25141 tgtgtcttta ggacaaaaac agttgctgac gattgcccga gcagtggtct ccgatcctaa
    25201 aatcttgatt ttagatgaag caacaagttc agtagacacc cgtttagaag cgttgattca
    25261 aaaagcaatg gatcgcgtga tggagggacg gacaagtttc gtcatcgctc accgtttatc
    25321 gacaatccgt gatgcagatc tgattctagt gatggatcaa ggtcagatca ttgaaaaagg
    25381 aacgcatgag agtttattgg cacaaggtgg attttatgaa aaactgtata acagccaatt
    25441 tgctgaggaa gcagaataag tcatataaga ataaaacagt ttaaaactta ttttttgttc
    25501 tctattcttg ataatggtaa agtaaagtta tcaagaaaag tgaggacacg gagatggaac
    25561 agtttttaaa tgaaaaaatt actgaagcca aaagagaagt tgatcgttta acgcacgtct
    25621 gtcagacgaa gccttttgat tcagctgatt tttttgaaaa tgagtttcaa atcgaacgct
    25681 tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa agtaatagaa gaaaatatag
    25741 atgtacaata aaaacaggta aaacgtcagt taccagatac tgtaaataga catctggcct
    25801 gagcaggttt tacctgtttt ttttcttatg aaataaatat atgctatgat acctttattc
    25861 aataggatta gtttctacaa aaaatgaaag gagaaaaata aatatggaac ggtttatagc
    25921 atcaaatgcc acagtgattg gtgatgtgac attgtcagaa gatgtgacga tctggtatca
    25981 agctgtttta cgcggggaca gtaattggat aaaaatagga caaagaacga atatccaaga
    26041 tggtacaatc atccatgtcg atcatgatgc acctgtagat attgcggaga atgtgacggt
    26101 aggacatcag tgcatgctac atggctgtac aatcgaaaaa ggggcgctga tcgggatggg
    26161 gaccacgata ttgaatcatg cggttattgg tgagaatagt ttgattggtg ctggatcatt
    26221 agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc cagctaaagt
    26281 gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc agcattatgt
    26341 cgagataggt cggatgcatg cgcaaggaaa attcaaggag caaagatagg cagtagcatc
    26401 cataaaataa aaaaggatgg tcagtttgag ttgaccatcc ttttttattt ggtttagata
    26461 agagagctta aaaaagcttc ctttgaaaca gattaattta atgaagaact gttttccatg
    26521 acttcatcga tcaatccata tgcttttgct tgttcagctg tcatatagtt gtcacgatct
    26581 gtatcttttt caatcacttc aagcggttgt ccagtgcgtt cagctaaaat tttgtttaag
    26641 cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc ttgcccttga
    26701 gcgccgccaa gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa tcgtttacct
    26761 tttgtaccag ctgtcagcaa gaagcttccc attgaagcag ccatacccat aacaattgtt
    26821 tggacatctg ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc tgtaactgaa
    26881 ccaccaggag aattgatata aagatagata tctttttcag aatcttgagc gtctaagaaa
    26941 agcaattggg cgatgatcga attagcaaga tcatctgtta cttggccgct taacatgatg
    27001 atacggtctt tcaataaacg tgagtagata tcgtaagctc tttcacctcg tgaagattgt
    27061 tcaatgactg taggaattaa attcataata gagttcctcc ttgatttcgt ttagaaatag
    27121 tgtaccataa tttagtttct aatacctaaa atgtagtata catttttggt caaaaaaggt
    27181 caaataaaaa acttaggcaa attagtcgca taaacccgct ttatggtatg ataagcagag
    27241 aaacaaaaca aggaggaaca acagattgat agttggtatt actggcctta tcggagtgct
    27301 gacaggtgct gtagttgtct atatcggcat gatactgcaa atgcgtcact tggaaaataa
    27361 agaacagcgt agacgtgaat tggaattgaa agtcaaagaa atcgagacgc tgaatcaatt
    27421 aaataaaaag atcaacgaga tcttgcaaaa acggacaacg ctgatggaag aatatgtcag
    27481 ctttgatgca tttgatgatt gttatatcac gattgacgat tttgcgtatc tccaatcatt
    27541 ttcagcgcaa aatagttttt atctgccaaa ttatatttta gacgagctat ttaagaatat
    27601 ctcccatcga aaagtgattt tatctccaga tgaaacaatg aagattggtg gatatgctta
    27661 taaaggcgga cgcgtgattt tagagaattt tacggatagt ctgactgaaa tgatcaatga
    27721 aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt ggtttcttct cgaaagatga
    27781 tgtttggagc tgatattttg aagcattgcc gttgcacatg agagcgtggt aatgctttct
    27841 ttttttctta atgatacaat agaactgaac ggagtgaaga aagatgttac aaatctatgc
    27901 ccatcgcgga agcagcggca cccacccaga aaatacgctt cctgcatttg cagaagctgt
    27961 acgagtaggt gcagatggta tcgaactaga tgtccatcta agcaaagatg gttatttgat
    28021 cgttatgcac gacgaagaag tggaccggac gactaacggt aaagggctga ttcgagaaaa
    28081 aacattggaa gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag aatttccagc
    28141 agctaaagtt cctgaattga gagaagtgtt aggactgctc ctccaaaaaa attaccgagg
    28201 gatgctaatg attgaaatta aaactgatca gtatgaatat cccggtatcg aagaaaaatt
    28261 agccgaaaca ttaaaaagta gagattggcc ttttcaacat gcatattgca gctttcattt
    28321 ggattctttg gaaaaaatcg cattactgga accggagata cagctggact tattgatgag
    28381 tacttctgat aaaaagatcc aactagcagc agaaacccct tatatagaag gagtacatcc
    28441 aaaaaatact tggttgttcg aacatccagt ccaggcagct gattttccaa aagcaattcg
    28501 tgcatggacg attaacgatg aagaagaaat ggccaaatgt caagaactca aaatagatgg
    28561 aataataact gattttccgg agaaagctct tcggtggaaa caatcaacga aaagtaggta
    28621 aaatatggaa aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa cagcgctaag
    28681 tgtggaactc gcacaactat ttggaggaga gatcatcagt ggtgattcat tgcaaatcta
    28741 taaaaatctg gatatcggta cagcaaaaat caaagaagaa gagatgaata atgttccgca
    28801 tcatttgatt gatgtgatcg aacctactca acgttactca gccgctgatt ttcagcattc
    28861 aggtcgacaa ttgattaccg agattgcgaa ccgagggcac ctacctatca tagctggtgg
    28921 tacgggtctc tatatccaat cccttctata tgattatcag ttaggagcga cagaagaagc
    28981 gaatcctgag attagaaaaa agtatgaaac gttagctgaa acaattggtt cccaagcatt
    29041 atggaatcag ttgaaagaaa aagacccggc agctgctgag aaaatccact ggaacaatca
    29101 acgcaaagtg ataagagctc tggaagtttt agagacgaca ggacatagta tcacggcacc
    29161 taaagaagag ccaaagcaat tgtatgatta cctcatgatt ggattagata cgaagcgtag
    29221 tattctttat gaacgaatca atcaaagagt cgatttgatg ctagcagaag gtttagtagc
    29281 agaagcgcgt aaggtgtatg agcttggaga agtccaggcc agtcaaggta ttggatataa
    29341 agaatttttc ccttattttg aagggaaaga aacattggaa gaagcaacag aacaagtcaa
    29401 actacattcg agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc gtttggatgc
    29461 acagtggttt gatcttataa gtgagccaaa tcaaaaaagt cagattgagc aaacaatcaa
    29521 aaaatggctg gaggcggaat aaaatggaag aaaaagtgat catcgtaggg gtagaaaccg
    29581 aagcaaacca gcgttacttt tcagagtcaa tggaagagct agttcagctg acgaataccg
    29641 catcaggaga agtcgtattc acaattacac aaaagagacc acaagtggat cggcaaacga
    29701 ttatcggtaa agggaaatta caagaattag ttcaacaagc ggatgcgcat gaagcggatt
    29761 tgatcatctt taatcatgag ctaactccga gacagagcca actgattacc gatgctgttg
    29821 gtcttccggt aattgatcgc gtgcagctta tcttagatat ttttgcgatg cgtgctcgtt
    29881 cgaaagaagg gaagctgcag gttgaacttg ctcagttaga atacctgctg cctcgtttag
    29941 tagggcaagg gaaaacatta tctcgtcttg gcggtgggat cggaacaaga gggccaggtg
    30001 aaacaaagtt agaaacagac cgccgacata tacggaataa aatttttgct gttaagaaag
    30061 aattgaaaga agtagaagct catcgagagc gcaatcgcca aaaaagaaaa aatagcgaga
    30121 tttttcagat tggacttatt ggctacacga atgccgggaa gtcaactatc ttgaatctat
    30181 tgacacaagc ggatacttat tcgaaagatc aattgtttgc aacgttagat ccattgacta
    30241 agagatggcg ttttgctgaa ggatttgaga ttactgtaac ggataccgtc gggttcatcc
    30301 aagacctgcc tacgcaactg atcgatgctt tccattctac attagaagaa agtcaaaata
    30361 tggatctttt gctacatgta gtcgatgcta gttctccaga tcgtatttta caggagcaga
    30421 ctgtcttaaa attgatggat gaactgaata tgaaagaaat gccgatactg actgtttaca
    30481 ataaggcaga ccagatcgac ccagcgatgt tcacgcctac gctattccca aatgtattga
    30541 tttctactca gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa cgtcagctga
    30601 tggaactaat gaccccttat acactttctg tgccaagcaa cgaaggacag aaattaagcg
    30661 agttgagaag acaaacaatg gttttggacg aagcgtacat ggaagaaaca aacgaatatg
    30721 aagttaaagg gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa taaaatttta
    30781 tttttttaa
//
LOCUS       DAYYCM010000028        30375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000028 DAYYCM010000000
VERSION     DAYYCM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.029"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1013
                     /locus_tag="ACHAY1_001337"
     CDS             144..1013
                     /locus_tag="ACHAY1_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8593342.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="ACHAY1_001338"
     CDS             1222..2247
                     /locus_tag="ACHAY1_001338"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8593343.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="ACHAY1_001339"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="ACHAY1_001339"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8593344.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3330..5033
                     /locus_tag="ACHAY1_001340"
     CDS             3330..5033
                     /locus_tag="ACHAY1_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8593345.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="ACHAY1_001341"
     CDS             5035..7068
                     /locus_tag="ACHAY1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8593346.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="ACHAY1_001342"
     CDS             7079..7519
                     /locus_tag="ACHAY1_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593347.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="ACHAY1_001343"
     CDS             complement(7719..8846)
                     /locus_tag="ACHAY1_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593348.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="ACHAY1_001344"
     CDS             complement(8849..9526)
                     /locus_tag="ACHAY1_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8593349.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="ACHAY1_001345"
     CDS             9790..11556
                     /locus_tag="ACHAY1_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8593350.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="ACHAY1_001346"
     CDS             11571..12008
                     /locus_tag="ACHAY1_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593351.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="ACHAY1_001347"
     CDS             12021..13172
                     /locus_tag="ACHAY1_001347"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8593352.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13234..13932)
                     /locus_tag="ACHAY1_001348"
     CDS             complement(13234..13932)
                     /locus_tag="ACHAY1_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8593353.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13925..15277)
                     /locus_tag="ACHAY1_001349"
     CDS             complement(13925..15277)
                     /locus_tag="ACHAY1_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8593354.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15500..16900)
                     /locus_tag="ACHAY1_001350"
     CDS             complement(15500..16900)
                     /locus_tag="ACHAY1_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8593355.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17061..17798
                     /locus_tag="ACHAY1_001351"
     CDS             17061..17798
                     /locus_tag="ACHAY1_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8593356.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17923..18402
                     /locus_tag="ACHAY1_001352"
     CDS             17923..18402
                     /locus_tag="ACHAY1_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8593357.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18399..19166
                     /locus_tag="ACHAY1_001353"
     CDS             18399..19166
                     /locus_tag="ACHAY1_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8593358.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19396..19965
                     /gene="lepB"
                     /locus_tag="ACHAY1_001354"
     CDS             19396..19965
                     /gene="lepB"
                     /locus_tag="ACHAY1_001354"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8593359.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20069..20671)
                     /locus_tag="ACHAY1_001355"
     CDS             complement(20069..20671)
                     /locus_tag="ACHAY1_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8593360.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20777..21007
                     /locus_tag="ACHAY1_001356"
     CDS             20777..21007
                     /locus_tag="ACHAY1_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593361.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            20998..21246
                     /locus_tag="ACHAY1_001357"
     CDS             20998..21246
                     /locus_tag="ACHAY1_001357"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593362.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21352..21936
                     /locus_tag="ACHAY1_001358"
     CDS             21352..21936
                     /locus_tag="ACHAY1_001358"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8593363.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21939..23012
                     /gene="prfA"
                     /locus_tag="ACHAY1_001359"
     CDS             21939..23012
                     /gene="prfA"
                     /locus_tag="ACHAY1_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8593364.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23005..23844
                     /gene="prmC"
                     /locus_tag="ACHAY1_001360"
     CDS             23005..23844
                     /gene="prmC"
                     /locus_tag="ACHAY1_001360"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8593365.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23837..24016
                     /locus_tag="ACHAY1_001361"
     CDS             23837..24016
                     /locus_tag="ACHAY1_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593366.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24038..25063
                     /locus_tag="ACHAY1_001362"
     CDS             24038..25063
                     /locus_tag="ACHAY1_001362"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8593367.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25254..26498
                     /gene="glyA"
                     /locus_tag="ACHAY1_001363"
     CDS             25254..26498
                     /gene="glyA"
                     /locus_tag="ACHAY1_001363"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8593368.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26651..27598
                     /locus_tag="ACHAY1_001364"
     CDS             26651..27598
                     /locus_tag="ACHAY1_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8593369.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27732..28361
                     /gene="upp"
                     /locus_tag="ACHAY1_001365"
     CDS             27732..28361
                     /gene="upp"
                     /locus_tag="ACHAY1_001365"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8593370.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28500..28742)
                     /locus_tag="ACHAY1_001366"
     CDS             complement(28500..28742)
                     /locus_tag="ACHAY1_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593371.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28863..28935
                     /locus_tag="ACHAY1_001367"
     tRNA            28863..28935
                     /locus_tag="ACHAY1_001367"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28896..28898,aa:Thr,seq:cgt)
     gene            complement(29054..29692)
                     /locus_tag="ACHAY1_001368"
     CDS             complement(29054..29692)
                     /locus_tag="ACHAY1_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8593372.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29815..>30375)
                     /locus_tag="ACHAY1_001369"
     CDS             complement(29815..>30375)
                     /locus_tag="ACHAY1_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8593373.1"
                     /translation="FELYNILSQKEVIENQIICHPIIYKSIFKYAFFASNDFQRMEIT
                     DCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKLMESYIGNTLFENCKMDYVNLSGSN
                     IKESTFEHSVLSSADFVDCSLTKIMFSTNDLENASFFDTDLKNIDLSSNSFEMIEVRS
                     EHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10342 a         5338 c         6147 g         8548 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc catgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccagta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagagaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttcgctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg
    13261 cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag
    13321 gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat
    13381 gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg
    13441 tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc
    13501 attgaattct tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa
    13561 cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta
    13621 gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata
    13681 gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc
    13741 ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact
    13801 aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag
    13861 gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc
    13921 ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa
    13981 gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga
    14041 ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg
    14101 caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga
    14161 tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt
    14221 ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca
    14281 tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc
    14341 cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt
    14401 tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt
    14461 agacattata cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg
    14521 ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga
    14581 agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca
    14641 agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac
    14701 gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga
    14761 agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta
    14821 caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat
    14881 ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat
    14941 ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg
    15001 atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct
    15061 ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa
    15121 cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta
    15181 gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt
    15241 ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt
    15301 gctgacgaat aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc
    15361 ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt
    15421 aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc
    15481 tcttacctat attcccattt taaccgataa taattttctc gcttggatat tcatagccta
    15541 gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa
    15601 taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta
    15661 aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt
    15721 gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa
    15781 agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt
    15841 tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc
    15901 gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga
    15961 tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg
    16021 taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat
    16081 ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta
    16141 acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt
    16201 ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca
    16261 aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag
    16321 tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg
    16381 catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa
    16441 aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag
    16501 acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc
    16561 gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa
    16621 ataatatttc cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg
    16681 atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat
    16741 ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca
    16801 atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc
    16861 ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat
    16921 agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat
    16981 aacaatacag ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa
    17041 gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc
    17101 aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag
    17161 taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac acattggaat
    17221 tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa
    17281 gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa
    17341 ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg
    17401 ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca
    17461 atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa
    17521 gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata
    17581 tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg
    17641 aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg
    17701 tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt
    17761 acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta
    17821 agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa
    17881 agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct
    17941 attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt
    18001 gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca
    18061 ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact
    18121 acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact
    18181 gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat
    18241 ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta
    18301 gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga
    18361 aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta
    18421 tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg
    18481 gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt
    18541 ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt
    18601 attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca
    18661 ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga
    18721 tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg
    18781 aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca
    18841 aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt
    18901 attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact
    18961 cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg
    19021 tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt
    19081 atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc
    19141 gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata
    19201 aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata
    19261 taacgagaaa gataagacga acacatcact tattctttcc tttcatttat aaacattgta
    19321 gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca
    19381 agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta
    19441 gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg
    19501 atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa
    19561 cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg
    19621 atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc
    19681 aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta
    19741 caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt
    19801 acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc
    19861 ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa
    19921 gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag
    19981 aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg
    20041 agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg
    20101 aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga
    20161 caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt
    20221 tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag
    20281 ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa
    20341 gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga
    20401 tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag
    20461 tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa
    20521 gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag
    20581 tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt
    20641 tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa
    20701 ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg
    20761 agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg
    20821 tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa
    20881 atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt
    20941 aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg
    21001 ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa
    21061 ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac
    21121 tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt
    21181 tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa
    21241 aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg
    21301 ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa
    21361 ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca
    21421 cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga
    21481 gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt
    21541 gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg
    21601 atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac
    21661 gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt
    21721 gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt
    21781 tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat
    21841 gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat
    21901 tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa
    21961 ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata
    22021 caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt
    22081 atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa
    22141 aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa
    22201 aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata
    22261 aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg
    22321 gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga
    22381 tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag
    22441 acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt
    22501 caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag
    22561 ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt
    22621 caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac
    22681 caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag
    22741 caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt
    22801 atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt
    22861 ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag
    22921 atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa
    22981 catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg
    23041 gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg
    23101 gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa
    23161 gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag
    23221 tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg
    23281 atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta
    23341 ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca
    23401 gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg
    23461 aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga
    23521 ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat
    23581 gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct
    23641 gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa
    23701 tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata
    23761 tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa
    23821 cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat
    23881 tataaaaaaa cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta
    23941 tccactattg acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca
    24001 aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga
    24061 aacagaattg caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac
    24121 cgaaacagta tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt
    24181 ttctgtaaaa gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca
    24241 agtaaaagag tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg
    24301 gcctggacca ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac
    24361 gggaggatta tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat
    24421 cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc
    24481 aacgactgca gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga
    24541 tggagctacg cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat
    24601 gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc
    24661 tcctgttgct attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat
    24721 gaaatacaaa cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc
    24781 aactgcagtt caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga
    24841 aattgcggat caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt
    24901 agaagcggcg gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg
    24961 attagatttg atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa
    25021 tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg
    25081 cattctttta gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta
    25141 agcgtaacta atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa
    25201 aataaaattg taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag
    25261 attacaaaac gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag
    25321 aacataattt ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac
    25381 aaggaagtat cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg
    25441 ggtgtgaatt tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg
    25501 gagcgaaatt tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc
    25561 tcgctttggt agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc
    25621 taacacatgg atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg
    25681 ttgatccgac aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc
    25741 aaccaaaatt gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat
    25801 tcagagaaat cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag
    25861 gattagtagc agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta
    25921 ccactcataa aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat
    25981 tagcaaaaaa aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg
    26041 tgattgcggg taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata
    26101 gtgaacaaat catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa
    26161 ccagagtcat ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag
    26221 gtataaatgg aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa
    26281 actcgattcc ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc
    26341 cagcaatcac tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg
    26401 tcaaagcatt acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac
    26461 gcgaattaac tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa
    26521 cagaaaagtt caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa
    26581 tatcggtgat gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg
    26641 aggaaaagtc atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc
    26701 aaatgcgcct catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga
    26761 agaagtacaa gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga
    26821 tactggcgaa tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa
    26881 attaccagtt attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca
    26941 tgataaattg atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata
    27001 tagtcgatca ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca
    27061 acagctgaat aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg
    27121 agattcagta ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg
    27181 cctcccaata tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga
    27241 ttcctatcat gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg
    27301 ggaccaatat acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc
    27361 aaatataaat gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt
    27421 attaagagat gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac
    27481 acaagtacgc ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac
    27541 aaaggcaacg cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc
    27601 tttgcttgtc cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag
    27661 tttgactagt caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga
    27721 gagcttaaaa aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa
    27781 ctattattcg ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg
    27841 ctatgttgat ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa
    27901 caccgatcac tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa
    27961 tcttgcgcgc aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag
    28021 tgggacatat cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca
    28081 aacttccaga agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag
    28141 gcggatcagc aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat
    28201 ttgtttgttt ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata
    28261 ttgacatcta tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg
    28321 gactagggga tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg
    28381 tgacacaact tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt
    28441 tttcacagct ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt
    28501 caaatctttt ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag
    28561 ttcgcatttt ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa
    28621 atcgttccag tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat
    28681 ttttcaccaa tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc
    28741 ataaaacctg cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa
    28801 ctaattttcc atcggactct ttacaatcag ccaaatagag tatataattt cttttggata
    28861 aggccagctt agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga
    28921 tccctatagc tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata
    28981 catctgtctt tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta
    29041 tttctataaa aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca
    29101 catgaactgc ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca
    29161 atcttgccac cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat
    29221 cataagtact gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa
    29281 tgctgaactt actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta
    29341 tcatgatagg caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg
    29401 ttaaacccca tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat
    29461 tgataaaaaa atatttggct atatttttga caggagcgac aggtttcgag aacatttttt
    29521 tcgtatcttt tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa
    29581 aaatgctaat aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag
    29641 tgcccaatat ccctttttta agttctattt tttttcctaa aatgttattc atgataataa
    29701 ctcctactat tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat
    29761 gtttttttat ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac
    29821 tacttttatt tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca
    29881 attttttaga tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat
    29941 attttttaaa tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat
    30001 tttagtgagg gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact
    30061 ttcttttatg tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt
    30121 gttcccaata taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac
    30181 gcgatgcaac atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca
    30241 gtcggtaatc tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga
    30301 tttgtaaata attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat
    30361 attatacagt tcaaa
//
LOCUS       DAYYCM010000029        30283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000029 DAYYCM010000000
VERSION     DAYYCM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.030"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACHAY1_001370"
     CDS             complement(<1..276)
                     /locus_tag="ACHAY1_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593374.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVK"
     gene            complement(264..893)
                     /gene="pyrE"
                     /locus_tag="ACHAY1_001371"
     CDS             complement(264..893)
                     /gene="pyrE"
                     /locus_tag="ACHAY1_001371"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8593375.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(900..1616)
                     /gene="pyrF"
                     /locus_tag="ACHAY1_001372"
     CDS             complement(900..1616)
                     /gene="pyrF"
                     /locus_tag="ACHAY1_001372"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8593376.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1613..2545)
                     /locus_tag="ACHAY1_001373"
     CDS             complement(1613..2545)
                     /locus_tag="ACHAY1_001373"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8593377.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2542..3318)
                     /locus_tag="ACHAY1_001374"
     CDS             complement(2542..3318)
                     /locus_tag="ACHAY1_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8593378.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3372..6557)
                     /gene="carB"
                     /locus_tag="ACHAY1_001375"
     CDS             complement(3372..6557)
                     /gene="carB"
                     /locus_tag="ACHAY1_001375"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8593379.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6562..7638)
                     /locus_tag="ACHAY1_001376"
     CDS             complement(6562..7638)
                     /locus_tag="ACHAY1_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8593380.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7643..8923)
                     /locus_tag="ACHAY1_001377"
     CDS             complement(7643..8923)
                     /locus_tag="ACHAY1_001377"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8593381.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8942..9868)
                     /locus_tag="ACHAY1_001378"
     CDS             complement(8942..9868)
                     /locus_tag="ACHAY1_001378"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8593382.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9881..11164)
                     /locus_tag="ACHAY1_001379"
     CDS             complement(9881..11164)
                     /locus_tag="ACHAY1_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8593383.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11178..11717)
                     /gene="pyrR"
                     /locus_tag="ACHAY1_001380"
     CDS             complement(11178..11717)
                     /gene="pyrR"
                     /locus_tag="ACHAY1_001380"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8593384.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11952..12863)
                     /locus_tag="ACHAY1_001381"
     CDS             complement(11952..12863)
                     /locus_tag="ACHAY1_001381"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8593385.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12860..13324)
                     /gene="lspA"
                     /locus_tag="ACHAY1_001382"
     CDS             complement(12860..13324)
                     /gene="lspA"
                     /locus_tag="ACHAY1_001382"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8593386.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13329..14027)
                     /locus_tag="ACHAY1_001383"
     CDS             complement(13329..14027)
                     /locus_tag="ACHAY1_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8593387.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(14014..14400)
                     /locus_tag="ACHAY1_001384"
     CDS             complement(14014..14400)
                     /locus_tag="ACHAY1_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593388.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14547..16217)
                     /locus_tag="ACHAY1_001385"
     CDS             complement(14547..16217)
                     /locus_tag="ACHAY1_001385"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8593389.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16400..16633
                     /locus_tag="ACHAY1_001386"
     CDS             16400..16633
                     /locus_tag="ACHAY1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8593390.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16732..17163)
                     /locus_tag="ACHAY1_001387"
     CDS             complement(16732..17163)
                     /locus_tag="ACHAY1_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8593391.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17242..17655
                     /locus_tag="ACHAY1_001388"
     CDS             17242..17655
                     /locus_tag="ACHAY1_001388"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8593392.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17693..18841)
                     /locus_tag="ACHAY1_001389"
     CDS             complement(17693..18841)
                     /locus_tag="ACHAY1_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8593393.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18963..19559)
                     /locus_tag="ACHAY1_001390"
     CDS             complement(18963..19559)
                     /locus_tag="ACHAY1_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8593394.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19759..22401)
                     /gene="alaS"
                     /locus_tag="ACHAY1_001391"
     CDS             complement(19759..22401)
                     /gene="alaS"
                     /locus_tag="ACHAY1_001391"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8593395.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22789..23220
                     /locus_tag="ACHAY1_001392"
     CDS             22789..23220
                     /locus_tag="ACHAY1_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593396.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23265..24614)
                     /locus_tag="ACHAY1_001393"
     CDS             complement(23265..24614)
                     /locus_tag="ACHAY1_001393"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8593397.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24684..25847)
                     /locus_tag="ACHAY1_001394"
     CDS             complement(24684..25847)
                     /locus_tag="ACHAY1_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8593398.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25976..26299
                     /locus_tag="ACHAY1_001395"
     CDS             25976..26299
                     /locus_tag="ACHAY1_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593399.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26482..27831)
                     /locus_tag="ACHAY1_001396"
     CDS             complement(26482..27831)
                     /locus_tag="ACHAY1_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8593400.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27900..28853)
                     /locus_tag="ACHAY1_001397"
     CDS             complement(27900..28853)
                     /locus_tag="ACHAY1_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8593401.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28955..30145)
                     /locus_tag="ACHAY1_001398"
     CDS             complement(28955..30145)
                     /locus_tag="ACHAY1_001398"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8593402.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8505 a         6654 c         5328 g         9796 t
ORIGIN      
        1 ctttaccatt tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga
       61 aaaaagagaa gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa
      121 aaaaatcaaa aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct
      181 cgcaatcagt ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat
      241 ttctctatct tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct
      301 tttagcagag ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat
      361 acagaataat tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct
      421 tttggcaact catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt
      481 gctgcttcag ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc
      541 atcttctgac cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc
      601 ttacttcgaa tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg
      661 attcctgctg ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg
      721 cttgctaatc cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta
      781 tcacagtaaa ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa
      841 aatactgctt caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt
      901 tagctccatt ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt
      961 ggtcgaccta taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact
     1021 cttgtctgat ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat
     1081 tcgtctcctg ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct
     1141 aatcctgctt ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg
     1201 attagttgat cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga
     1261 agtagcggac gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc
     1321 atcatacgaa taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat
     1381 ccccgcattg ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca
     1441 ttatgaccag cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc
     1501 attccgattt tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc
     1561 tcttgcctag ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt
     1621 cacgttcctc tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca
     1681 ttcgcacagg gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg
     1741 tccctatagc tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct
     1801 gcacgccgcc catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac
     1861 gaatagcgac aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg
     1921 gttttcttgt cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat
     1981 ctgcccctcc agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca
     2041 atttgacata gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa
     2101 catcagaatc tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca
     2161 gctcgattgc tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt
     2221 cttcacaagc accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg
     2281 gaagaagttt atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca
     2341 tgccagatgg tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg
     2401 ttgccttgac catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc
     2461 caaaaccgaa acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt
     2521 tgaccgctaa aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc
     2581 gtcacaaact ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc
     2641 atagcaagcc cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg
     2701 cgaagaaaac agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc
     2761 tgtaggttcc gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt
     2821 tccatcgtca gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac
     2881 gacttcatgt gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg
     2941 ttttgataac tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc
     3001 tcctgtatga agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg
     3061 gtctccggca gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat
     3121 acggcaagta tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa
     3181 gtctgcacgc ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag
     3241 atcacctttc aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc
     3301 catcatttct tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca
     3361 tgtgctcgcc gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc
     3421 gcatctgctg tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg
     3481 atgatgaaac cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact
     3541 tgtgttttac catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt
     3601 ttcgctactg tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt
     3661 cctatcagac tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt
     3721 gtttcgtccg caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct
     3781 tcgaatgcct tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc
     3841 gatttcattt caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca
     3901 ggtgctttaa catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc
     3961 tttagtttct ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc
     4021 ttacttaaga atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca
     4081 ttttcatgga tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag
     4141 cggatcgtgt aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac
     4201 acagccattg aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg
     4261 atgagtactg tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa
     4321 cggtccacta gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct
     4381 aaatctcgct ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga
     4441 acaagaactg gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca
     4501 gtatccccta aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga
     4561 ttttccgcac gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc
     4621 aaaggttcgg ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc
     4681 tgttctagct cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta
     4741 tccgaaatag aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg
     4801 tatcctgcct gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct
     4861 tgtccgatac ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa
     4921 ctttcatttt catattcata ggaactataa aaataaggtg tttttgattc gaattccgct
     4981 gcacaagtgt caaccatctt atatacagga atcaaatcat tttcttttct catctggcga
     5041 acggcttctt cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt
     5101 ttcgctgttt tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct
     5161 acgatatgca acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct
     5221 tctatggtgt accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct
     5281 cgaaccactt tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata
     5341 tggtgtgtac cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga
     5401 ccgatagcca tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt
     5461 tcaaacttat caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca
     5521 aattctgcaa aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc
     5581 gctattttag cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat
     5641 gaacgagaaa cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga
     5701 gcgagttgga cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca
     5761 tcacgaagca tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa
     5821 tcacctgtat ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta
     5881 tcagccgaat ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca
     5941 atcaaacatt gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct
     6001 tcttcatttt cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg
     6061 acaggatagc cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca
     6121 gactcgggga ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct
     6181 gcttgatcga tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata
     6241 cctgatgctg ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg
     6301 agagcatctg gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg
     6361 atatatactt gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact
     6421 aaaaccactt catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca
     6481 aattctgcgg cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata
     6541 tctgtacgtt tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc
     6601 atcaaataaa tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact
     6661 gaaagcggga taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg
     6721 agttacgatg agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg
     6781 agaagtaaaa tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg
     6841 tccgaatttc attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag
     6901 acaaatacca aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg
     6961 cacatctttt ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa
     7021 aatcgtctcg gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa
     7081 ttctcgtaaa atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc
     7141 tggacttgga taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt
     7201 tgctttcagc tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt
     7261 catcgttccg acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc
     7321 aggaattcct ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc
     7381 tacacgtgca tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc
     7441 atctctattt accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt
     7501 aaaactttga tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc
     7561 tccgaagaca ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa
     7621 tataagcaaa cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa
     7681 aagtcatttg tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg
     7741 ataaaaagtc ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag
     7801 cgggggcacc aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg
     7861 ccaaccaatt gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga
     7921 tcagttgaaa cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc
     7981 ttttttcttc taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa
     8041 gcaagccttc gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc
     8101 aaaagccgaa atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt
     8161 ctgctgttac gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag
     8221 tagaaacatg gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag
     8281 caatttgaga agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct
     8341 tgccagcgtg cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg
     8401 ctttattgtt ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac
     8461 ctacaccgtc atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta
     8521 atttctcact tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat
     8581 cttttctaat gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta
     8641 aattcggcat cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt
     8701 cgatcgtctc cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac
     8761 caggtgtgat caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc
     8821 ctgtaccgat tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat
     8881 tttttcgtgt attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata
     8941 attacgcttt tccatttagc actgcttcta agattgccat tctgacgaat actccattgc
     9001 tcatttgctg aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat
     9061 cacggttaac tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct
     9121 caactgttaa cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat
     9181 ggcgttcgtg ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca
     9241 atggcatata atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga
     9301 agaatacttg tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt
     9361 gagtgatatc acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa
     9421 tggtcatcaa atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga
     9481 taatcgaaca ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat
     9541 gacggataac cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg
     9601 tttccccttt ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt
     9661 ttttctccgc tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg
     9721 tagcaaagta ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc
     9781 tgcggatcaa gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta
     9841 agctgatacg ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg
     9901 aagtttctgg taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg
     9961 acaatgtaaa tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg
    10021 aagcaatcaa cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca
    10081 ttccacttgc ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag
    10141 ggatacttaa aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg
    10201 cagctccacc gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt
    10261 caccgtaact tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat
    10321 cacctgtcaa cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat
    10381 tcaataccat aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg
    10441 ttgtgattgc tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag
    10501 gaacagcaaa ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg
    10561 ctacgatgta tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta
    10621 gccacatgtt gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga
    10681 aatcatacgt gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc
    10741 cgataaccat gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg
    10801 aaccgatctt tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc
    10861 cttgtgcaat cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag
    10921 cgaagctgct tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg
    10981 tacctagtcc tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta
    11041 acacggttgc tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg
    11101 gggttgggcg atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg
    11161 acatgtttct ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca
    11221 agctcttgca tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca
    11281 taatctgcac ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt
    11341 tttcttggtc tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta
    11401 tacaaaacat catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa
    11461 gaatgaagtt ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc
    11521 agttctccta caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat
    11581 gcaatgtaga ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt
    11641 ctctcgataa tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact
    11701 acttcttttg cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta
    11761 agaggacagg aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt
    11821 caatgagcaa tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc
    11881 acgggactaa acttaaagga attctattaa gttttatgct ttcgttgtta attactatac
    11941 cgttatctaa attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact
    12001 tctggaagag gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac
    12061 atttgcgcat ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga
    12121 tctccagcta acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg
    12181 gtttctagtt ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt
    12241 gtgacagccg acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg
    12301 ccgattggcg cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca
    12361 tatttgcgca aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca
    12421 tttttggcaa ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga
    12481 cgaataacgc cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt
    12541 gtgccatgcg tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc
    12601 gctacatctt catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat
    12661 gtttctggtt cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct
    12721 ttgactggct gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa
    12781 cgactgtaat cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga
    12841 atcgtcgttt taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa
    12901 ttaacataca aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc
    12961 tcataaaatc tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat
    13021 tgccaatcgc accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt
    13081 cacgattttt aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa
    13141 agaaccacat cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca
    13201 gtgacaaaat acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc
    13261 accatttgac caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta
    13321 acaaaaaatc aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc
    13381 agattaccat cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca
    13441 cgaaacatcg cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg
    13501 tactttccgt tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct
    13561 ttttggcttt cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat
    13621 tttcctttat tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt
    13681 tcaagtaacg gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg
    13741 gtttcaggct gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt
    13801 ggctctgcaa tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga
    13861 gcaagctgta aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg
    13921 cgtctgacaa gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat
    13981 ttttcgattc gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt
    14041 gatcaaatat gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa
    14101 aaaactgaaa aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa
    14161 cggatagatt tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt
    14221 cactgtataa acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc
    14281 cagatcaagc gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct
    14341 tagctgaatc gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat
    14401 cataccctcc tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct
    14461 agtgtaaaca aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct
    14521 ccttcgatgg tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc
    14581 acgtccatat tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca
    14641 gtcaaggcta ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg
    14701 aaatcttccg gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc
    14761 atacaaatcg gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct
    14821 tttttgctga aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt
    14881 tcttgtatcg tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc
    14941 aatcgtacta cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc
    15001 gctctcttgg caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg
    15061 tctgtatctg ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata
    15121 ttacgtatat ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt
    15181 ttaagttcat cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc
    15241 accacagcat ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg
    15301 ccaagatctg caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc
    15361 ttggtagcta aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca
    15421 aaagctggtg ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt
    15481 aatgccagag cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta
    15541 tagccgacaa cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac
    15601 aagatagcca tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct
    15661 tggattggcc cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact
    15721 acccgtttcc aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga
    15781 ttatctaaaa gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta
    15841 aaatgcaagt tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca
    15901 cctttgattc ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta
    15961 ttgattttat tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct
    16021 ggtgttggat tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga
    16081 ttgattccag aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc
    16141 ttaagatcta atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt
    16201 tgcagatcat tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc
    16261 tttgttaaga tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg
    16321 tttttaattt atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt
    16381 tagaaaggtc gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg
    16441 atacggcttt atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga
    16501 agaagaggga ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg
    16561 gaatcgagga acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag
    16621 tatgcatgaa tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca
    16681 ctcttttctt ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt
    16741 tctttttttc gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg
    16801 atcaaaaagg actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc
    16861 ttgcaacatc atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga
    16921 tatttctcgg tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat
    16981 aaaagaacgc cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct
    17041 tttccaataa ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa
    17101 ggtcaatgac caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt
    17161 cattctctaa cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa
    17221 caaaaaggag aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac
    17281 cctggtccta gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct
    17341 atcccgcttt ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta
    17401 gaatttctaa agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa
    17461 acactagttt ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt
    17521 caaagaatcc aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa
    17581 aacaaaaata aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa
    17641 aaaaagactc tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata
    17701 aaagataatt gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt
    17761 gactacgtaa gattctttct agttctgtat caacaaactg gaatttctca gggaccacta
    17821 catcaaattg cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg
    17881 tacctgtttc aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat
    17941 caacaggcat tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt
    18001 tttctatcag atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga
    18061 tgtggactga tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc
    18121 caagtatatc atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg
    18181 ggcgacttcc taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga
    18241 tatataatgc taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga
    18301 ttgctgctga aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg
    18361 tagctctcaa tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg
    18421 caaatttcaa aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat
    18481 tttctggatg gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa
    18541 tgatgatcga aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt
    18601 cctgatctgc tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag
    18661 tatctgatag actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga
    18721 tcattagctt agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta
    18781 ctcctttttt tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca
    18841 agttttttac cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt
    18901 tttcctctcc tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt
    18961 cattattctg gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg
    19021 attcccgcac ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta
    19081 aataaaagag cagagtctac accataggca tctgccgttg cacctgtata aaataatccc
    19141 atgaatccta acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag
    19201 ctgatcgtta ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag
    19261 ccaacaagta ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat
    19321 ggcgttgttg gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc
    19381 accatgccat cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt
    19441 gttaaactaa tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg
    19501 atgattgcta acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt
    19561 ttttcacccc tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag
    19621 accatccgaa tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca
    19681 agacttgttt gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt
    19741 ctgtcaaacg taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc
    19801 tggaatacca gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc
    19861 accgcctact ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt
    19921 tgattctttc gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac
    19981 aagaatatct gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat
    20041 atctttcact tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt
    20101 gaatacatca ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg
    20161 tagctctctt acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg
    20221 tgatttaaca attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta
    20281 cgcttcttta cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga
    20341 aacgattttg aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc
    20401 gattgaccag ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa
    20461 agccattgca cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat
    20521 agcttcccag attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat
    20581 ttgaccaaag tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg
    20641 atttgcatgc tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg
    20701 gtttttgatg atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc
    20761 actcattggt gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac
    20821 atctgtaaca actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac
    20881 ttgcccaccc atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc
    20941 tgcgacttca ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc
    21001 ttgttctgtg tatccaatga atttactttc tactttgata tctgtcaata aagcagattg
    21061 tacccccatt gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat
    21121 ttctttttca aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt
    21181 taattctacc gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt
    21241 cgtcccgttt tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt
    21301 gattgtttca ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg
    21361 ttgaagtact tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata
    21421 taagaatgct tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg
    21481 gcgtaagaca taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga
    21541 aagggcgcga atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc
    21601 atattttact tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt
    21661 ctcaaagttc gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt
    21721 atcaatgttt ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga
    21781 aaatactaag ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg
    21841 atcgtcttct gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg
    21901 accacatggg cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag
    21961 tggcaggcca acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt
    22021 tacataaagt ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc
    22081 ccagtggatg gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa
    22141 catcgtatgg tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt
    22201 ttgggcattt gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa
    22261 tgttgcaaca ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc
    22321 actaggttca atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg
    22381 tgcactactt aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca
    22441 ccatcacagc caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat
    22501 tacctcttaa actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg
    22561 agcaatttga ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa
    22621 aaaacaaatc atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa
    22681 tatctcaatt tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag
    22741 aataaaggaa tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata
    22801 gttggagcgg atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta
    22861 ttagaacaag ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc
    22921 tattatctat ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca
    22981 cctacttgct tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta
    23041 ggcggtcagt tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt
    23101 tatgtagtag ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc
    23161 acagaacctt ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa
    23221 aaacaagaga ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt
    23281 ttcttatttt tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta
    23341 tttttttctg caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt
    23401 ttcttcttca caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg
    23461 gcacgtctat tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca
    23521 aatgagacgc ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac
    23581 gtaatggccg ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag
    23641 tctagatctt ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct
    23701 ctagcagcta aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc
    23761 actcgttttc tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg
    23821 tttttcaagt aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct
    23881 aaataaggat gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt
    23941 gttgaaatca gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca
    24001 atgattggat tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca
    24061 gaaaaaacta aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt
    24121 aaaaaaccca tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga
    24181 atcttcagcg cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc
    24241 tgtggctgtt ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa
    24301 ttcgagacac ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc
    24361 tgtgcagcta gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca
    24421 ttgatttttt caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat
    24481 tgcccaatga tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg
    24541 ggctgtttga agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata
    24601 tcaaaattat tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac
    24661 acagacaaag cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc
    24721 gtgctaacag tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa
    24781 aatgatagca agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg
    24841 aatactgcac tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac
    24901 tgccccaacc agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat
    24961 gcttttaaag aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg
    25021 ccaagtgtcc atccattggt tataaacggt acaatcataa agtatacccc tacttctaat
    25081 aaaagccacc agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc
    25141 gccgctacca aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt
    25201 cgagtcaatg agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta
    25261 ggaagcataa atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt
    25321 gtcactaaat cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca
    25381 cttagctgcg tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata
    25441 aaagaaagcc ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta
    25501 aaagctggtt ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac
    25561 aagaccaata ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc
    25621 tttggccctg tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa
    25681 tacgcacaca ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat
    25741 gttccatact ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat
    25801 ggaaaaacaa tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt
    25861 gattctttgt atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct
    25921 attatttctc atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct
    25981 gaatatgtac tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc
    26041 ttatgtaaga aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg
    26101 tttcatgcta gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat
    26161 gactccacat gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat
    26221 tttcatttct ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca
    26281 gttacgcaaa tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt
    26341 gctatttcat cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca
    26401 tttcttccac gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct
    26461 aataaaattc gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc
    26521 aagtgtcaat actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac
    26581 gataaaagtc cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta
    26641 tggtcgaaac aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt
    26701 ataaaaaatc caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat
    26761 catcatgcca atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt
    26821 atcatgaagt ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata
    26881 agggagacgc attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat
    26941 accaactgtg accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc
    27001 cgcattgctg tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac
    27061 taataaataa acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt
    27121 cgccggcttt ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa
    27181 catcgttgct agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg
    27241 gatcggaaac agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac
    27301 aaacaaagga ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga
    27361 acctaaaaaa ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat
    27421 tgactgggaa agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc
    27481 tacatttgct ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt
    27541 tccgtaaatc cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag
    27601 ttcagctcct gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat
    27661 atgcaaactg gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc
    27721 accgatcact gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa
    27781 ttcattttct atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc
    27841 cctttcatac catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt
    27901 tataccgtct ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct
    27961 aagtcgtatt tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt
    28021 ggccaagatt ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct
    28081 ggtatgattt tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata
    28141 ttgaaggcat ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca
    28201 tcgatcgcat ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg
    28261 tactttcctt ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct
    28321 cctggcaagg ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca
    28381 tatttcacat aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg
    28441 gttgtaggac gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac
    28501 gcgccgattg aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca
    28561 ttgaccagcc cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct
    28621 gacaaaagag cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc
    28681 attttttcgt aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca
    28741 ggcatgcgta tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg
    28801 atcaactctg aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc
    28861 ctccctgatc ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc
    28921 tctaatttta aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta
    28981 ttttttgata aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca
    29041 tatttctgat cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa
    29101 tcccatgttc gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga
    29161 ctggagtaat aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt
    29221 taaaataatt agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag
    29281 taatcgaagc caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg
    29341 gccgacttct agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa
    29401 caacacctat agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa
    29461 aatgcttgac ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt
    29521 aggtgatcag tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc
    29581 catccatgga gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta
    29641 tcgcttcctt tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt
    29701 ttgctccgga taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga
    29761 tagcatcttc ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt
    29821 gaaaggcgat tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat
    29881 ggattttcag cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg
    29941 ttgcttcaat acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg
    30001 atgctagatt gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta
    30061 gtatgtcgat cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat
    30121 acattgttag ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac
    30181 ataccaaaca tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg
    30241 cttacactga ttatgagaat tatttaacca cattttttat tta
//
LOCUS       DAYYCM010000030        29333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000030 DAYYCM010000000
VERSION     DAYYCM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.031"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..565)
                     /locus_tag="ACHAY1_001399"
     CDS             complement(131..565)
                     /locus_tag="ACHAY1_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8593403.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(665..1513)
                     /locus_tag="ACHAY1_001400"
     CDS             complement(665..1513)
                     /locus_tag="ACHAY1_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8593404.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1902..3668
                     /locus_tag="ACHAY1_001401"
     CDS             1902..3668
                     /locus_tag="ACHAY1_001401"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8593405.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            3770..5029
                     /locus_tag="ACHAY1_001402"
     CDS             3770..5029
                     /locus_tag="ACHAY1_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8593406.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            5176..6477
                     /locus_tag="ACHAY1_001403"
     CDS             5176..6477
                     /locus_tag="ACHAY1_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593407.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            6480..7331
                     /locus_tag="ACHAY1_001404"
     CDS             6480..7331
                     /locus_tag="ACHAY1_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8593408.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            7344..8159
                     /locus_tag="ACHAY1_001405"
     CDS             7344..8159
                     /locus_tag="ACHAY1_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8593409.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            8310..9476
                     /locus_tag="ACHAY1_001406"
     CDS             8310..9476
                     /locus_tag="ACHAY1_001406"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8593410.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            9706..10353
                     /locus_tag="ACHAY1_001407"
     CDS             9706..10353
                     /locus_tag="ACHAY1_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8593411.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            10437..11381
                     /locus_tag="ACHAY1_001408"
     CDS             10437..11381
                     /locus_tag="ACHAY1_001408"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8593412.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            11516..12967
                     /locus_tag="ACHAY1_001409"
     CDS             11516..12967
                     /locus_tag="ACHAY1_001409"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8593413.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            12988..13824
                     /locus_tag="ACHAY1_001410"
     CDS             12988..13824
                     /locus_tag="ACHAY1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8593414.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            13824..14483
                     /locus_tag="ACHAY1_001411"
     CDS             13824..14483
                     /locus_tag="ACHAY1_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8593415.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            14609..15298
                     /locus_tag="ACHAY1_001412"
     CDS             14609..15298
                     /locus_tag="ACHAY1_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593416.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            15304..16260
                     /locus_tag="ACHAY1_001413"
     CDS             15304..16260
                     /locus_tag="ACHAY1_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8593417.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            16304..16816
                     /locus_tag="ACHAY1_001414"
     CDS             16304..16816
                     /locus_tag="ACHAY1_001414"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593418.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(17072..17620)
                     /locus_tag="ACHAY1_001415"
     CDS             complement(17072..17620)
                     /locus_tag="ACHAY1_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8593419.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(17709..18890)
                     /locus_tag="ACHAY1_001416"
     CDS             complement(17709..18890)
                     /locus_tag="ACHAY1_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8593420.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(19126..20634)
                     /locus_tag="ACHAY1_001417"
     CDS             complement(19126..20634)
                     /locus_tag="ACHAY1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8593421.1"
                     /translation="MKKNGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            20793..20984
                     /locus_tag="ACHAY1_001418"
     CDS             20793..20984
                     /locus_tag="ACHAY1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593422.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            21187..22104
                     /locus_tag="ACHAY1_001419"
     CDS             21187..22104
                     /locus_tag="ACHAY1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8593423.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            22097..23170
                     /locus_tag="ACHAY1_001420"
     CDS             22097..23170
                     /locus_tag="ACHAY1_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593424.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            23318..23836
                     /locus_tag="ACHAY1_001421"
     CDS             23318..23836
                     /locus_tag="ACHAY1_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593425.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            23817..24209
                     /locus_tag="ACHAY1_001422"
     CDS             23817..24209
                     /locus_tag="ACHAY1_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8593426.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            24285..25220
                     /locus_tag="ACHAY1_001423"
     CDS             24285..25220
                     /locus_tag="ACHAY1_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8593427.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            25353..26132
                     /locus_tag="ACHAY1_001424"
     CDS             25353..26132
                     /locus_tag="ACHAY1_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8593428.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            26125..26442
                     /locus_tag="ACHAY1_001425"
     CDS             26125..26442
                     /locus_tag="ACHAY1_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8593429.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            26686..28296
                     /locus_tag="ACHAY1_001426"
     CDS             26686..28296
                     /locus_tag="ACHAY1_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8593430.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            28453..29301
                     /locus_tag="ACHAY1_001427"
     CDS             28453..29301
                     /locus_tag="ACHAY1_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8593431.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         9554 a         5210 c         5800 g         8769 t
ORIGIN      
        1 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
       61 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
      121 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
      181 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
      241 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
      301 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
      361 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
      421 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
      481 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
      541 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
      601 agattatttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
      661 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
      721 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
      781 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
      841 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
      901 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
      961 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
     1021 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
     1081 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
     1141 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
     1201 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
     1261 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
     1321 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
     1381 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
     1441 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
     1501 tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc ttattataac
     1561 ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta
     1621 tgtttcattc ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag
     1681 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
     1741 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
     1801 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
     1861 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
     1921 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
     1981 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
     2041 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
     2101 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
     2161 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
     2221 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
     2281 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
     2341 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
     2401 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
     2461 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
     2521 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
     2581 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
     2641 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
     2701 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
     2761 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
     2821 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
     2881 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
     2941 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
     3001 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
     3061 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
     3121 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
     3181 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
     3241 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
     3301 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
     3361 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
     3421 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
     3481 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
     3541 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
     3601 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
     3661 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
     3721 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
     3781 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
     3841 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
     3901 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
     3961 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
     4021 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
     4081 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
     4141 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
     4201 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
     4261 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
     4321 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
     4381 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
     4441 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
     4501 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
     4561 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
     4621 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
     4681 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
     4741 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
     4801 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
     4861 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
     4921 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
     4981 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
     5041 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
     5101 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
     5161 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
     5221 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
     5281 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
     5341 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
     5401 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
     5461 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
     5521 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
     5581 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
     5641 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
     5701 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
     5761 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
     5821 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
     5881 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
     5941 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
     6001 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
     6061 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
     6121 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
     6181 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
     6241 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
     6301 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
     6361 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
     6421 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
     6481 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
     6541 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
     6601 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
     6661 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
     6721 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
     6781 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
     6841 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
     6901 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
     6961 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
     7021 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
     7081 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
     7141 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
     7201 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
     7261 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
     7321 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
     7381 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
     7441 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
     7501 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
     7561 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
     7621 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
     7681 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
     7741 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
     7801 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
     7861 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
     7921 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
     7981 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
     8041 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
     8101 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
     8161 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
     8221 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
     8281 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
     8341 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
     8401 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
     8461 aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg
     8521 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
     8581 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
     8641 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
     8701 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
     8761 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
     8821 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
     8881 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
     8941 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
     9001 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
     9061 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
     9121 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
     9181 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
     9241 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
     9301 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
     9361 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
     9421 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
     9481 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
     9541 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
     9601 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
     9661 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
     9721 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
     9781 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
     9841 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
     9901 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
     9961 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
    10021 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
    10081 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
    10141 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
    10201 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
    10261 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
    10321 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
    10381 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
    10441 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
    10501 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
    10561 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
    10621 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
    10681 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
    10741 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
    10801 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
    10861 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
    10921 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
    10981 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
    11041 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
    11101 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
    11161 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
    11221 atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa gaagggccgg
    11281 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
    11341 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
    11401 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
    11461 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
    11521 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
    11581 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
    11641 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    11701 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    11761 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    11821 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    11881 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    11941 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    12001 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    12061 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    12121 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    12181 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    12241 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    12301 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    12361 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    12421 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    12481 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    12541 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    12601 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    12661 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    12721 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    12781 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    12841 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    12901 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    12961 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    13021 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    13081 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    13141 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    13201 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    13261 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    13321 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    13381 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    13441 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    13501 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    13561 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    13621 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    13681 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    13741 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    13801 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    13861 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    13921 ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    13981 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    14041 tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg gcttcagtca
    14101 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    14161 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    14221 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    14281 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    14341 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    14401 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    14461 gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    14521 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    14581 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    14641 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    14701 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    14761 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    14821 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    14881 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    14941 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    15001 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    15061 gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag aattgaaaag
    15121 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    15181 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    15241 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    15301 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    15361 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    15421 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
    15481 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    15541 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    15601 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    15661 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    15721 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    15781 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    15841 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    15901 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    15961 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    16021 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    16081 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    16141 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    16201 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    16261 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    16321 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    16381 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    16441 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    16501 tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    16561 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    16621 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    16681 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    16741 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    16801 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    16861 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    16921 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    16981 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct
    17041 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    17101 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    17161 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    17221 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    17281 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    17341 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    17401 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    17461 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    17521 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    17581 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    17641 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    17701 tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    17761 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    17821 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    17881 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    17941 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    18001 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    18061 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    18121 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    18181 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    18241 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    18301 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    18361 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    18421 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    18481 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    18541 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    18601 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    18661 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    18721 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    18781 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    18841 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    18901 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    18961 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    19021 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    19081 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    19141 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    19201 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    19261 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    19321 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    19381 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    19441 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    19501 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    19561 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    19621 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    19681 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    19741 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    19801 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    19861 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    19921 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    19981 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    20041 atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc
    20101 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    20161 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
    20221 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    20281 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    20341 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    20401 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    20461 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    20521 aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    20581 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccattttttt tcactaggtt
    20641 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    20701 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    20761 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    20821 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    20881 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    20941 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    21001 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    21061 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    21121 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    21181 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    21241 aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    21301 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    21361 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    21421 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    21481 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    21541 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    21601 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    21661 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    21721 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    21781 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    21841 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    21901 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    21961 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    22021 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    22081 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    22141 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    22201 tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac
    22261 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    22321 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    22381 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    22441 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    22501 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    22561 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    22621 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    22681 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    22741 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    22801 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    22861 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    22921 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    22981 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    23041 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    23101 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    23161 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    23221 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    23281 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    23341 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    23401 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    23461 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    23521 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    23581 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    23641 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    23701 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    23761 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    23821 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    23881 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    23941 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa
    24001 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    24061 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    24121 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    24181 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    24241 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    24301 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    24361 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    24421 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    24481 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    24541 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    24601 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    24661 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    24721 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    24781 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    24841 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    24901 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    24961 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    25021 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    25081 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    25141 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    25201 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    25261 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    25321 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    25381 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    25441 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    25501 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    25561 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    25621 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    25681 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    25741 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    25801 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    25861 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    25921 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    25981 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    26041 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    26101 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    26161 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    26221 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    26281 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    26341 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    26401 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    26461 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    26521 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    26581 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    26641 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    26701 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    26761 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    26821 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    26881 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    26941 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    27001 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    27061 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    27121 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    27181 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    27241 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    27301 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    27361 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    27421 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    27481 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    27541 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    27601 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    27661 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    27721 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    27781 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    27841 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    27901 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    27961 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    28021 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    28081 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    28141 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    28201 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    28261 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    28321 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    28381 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    28441 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    28501 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    28561 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    28621 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    28681 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    28741 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    28801 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    28861 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat
    28921 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    28981 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    29041 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    29101 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    29161 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    29221 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    29281 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DAYYCM010000031        29160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000031 DAYYCM010000000
VERSION     DAYYCM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.032"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1063)
                     /locus_tag="ACHAY1_001428"
     CDS             complement(14..1063)
                     /locus_tag="ACHAY1_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8593432.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1145..1834)
                     /locus_tag="ACHAY1_001429"
     CDS             complement(1145..1834)
                     /locus_tag="ACHAY1_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8593433.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1940..2308)
                     /gene="rplL"
                     /locus_tag="ACHAY1_001430"
     CDS             complement(1940..2308)
                     /gene="rplL"
                     /locus_tag="ACHAY1_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8593434.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2361..2864)
                     /gene="rplJ"
                     /locus_tag="ACHAY1_001431"
     CDS             complement(2361..2864)
                     /gene="rplJ"
                     /locus_tag="ACHAY1_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8593435.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3068..3195)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3274..3963)
                     /gene="rplA"
                     /locus_tag="ACHAY1_001432"
     CDS             complement(3274..3963)
                     /gene="rplA"
                     /locus_tag="ACHAY1_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8593436.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4067..4489)
                     /gene="rplK"
                     /locus_tag="ACHAY1_001433"
     CDS             complement(4067..4489)
                     /gene="rplK"
                     /locus_tag="ACHAY1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8593437.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4832..5500
                     /gene="sdaAB"
                     /locus_tag="ACHAY1_001434"
     CDS             4832..5500
                     /gene="sdaAB"
                     /locus_tag="ACHAY1_001434"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8593438.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5513..6385
                     /gene="sdaAA"
                     /locus_tag="ACHAY1_001435"
     CDS             5513..6385
                     /gene="sdaAA"
                     /locus_tag="ACHAY1_001435"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8593439.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6476..7576)
                     /locus_tag="ACHAY1_001436"
                     /pseudo
     CDS             complement(6476..7576)
                     /locus_tag="ACHAY1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7668..8555)
                     /locus_tag="ACHAY1_001437"
     CDS             complement(7668..8555)
                     /locus_tag="ACHAY1_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8593440.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8632..9177)
                     /gene="nusG"
                     /locus_tag="ACHAY1_001438"
     CDS             complement(8632..9177)
                     /gene="nusG"
                     /locus_tag="ACHAY1_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8593441.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9278..9448)
                     /gene="secE"
                     /locus_tag="ACHAY1_001439"
     CDS             complement(9278..9448)
                     /gene="secE"
                     /locus_tag="ACHAY1_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8593442.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9472..9624)
                     /gene="rpmG"
                     /locus_tag="ACHAY1_001440"
     CDS             complement(9472..9624)
                     /gene="rpmG"
                     /locus_tag="ACHAY1_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8593443.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9786..10430
                     /gene="cbpA"
                     /locus_tag="ACHAY1_001441"
     CDS             9786..10430
                     /gene="cbpA"
                     /locus_tag="ACHAY1_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8593444.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10486..10989)
                     /locus_tag="ACHAY1_001442"
     CDS             complement(10486..10989)
                     /locus_tag="ACHAY1_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8593445.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11002..11048)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11586..12551
                     /locus_tag="ACHAY1_001443"
     CDS             11586..12551
                     /locus_tag="ACHAY1_001443"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8593446.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12650..13822)
                     /locus_tag="ACHAY1_001444"
     CDS             complement(12650..13822)
                     /locus_tag="ACHAY1_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593447.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13809..14954)
                     /locus_tag="ACHAY1_001445"
     CDS             complement(13809..14954)
                     /locus_tag="ACHAY1_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593448.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14968..15900)
                     /locus_tag="ACHAY1_001446"
     CDS             complement(14968..15900)
                     /locus_tag="ACHAY1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593449.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16017..17096
                     /locus_tag="ACHAY1_001447"
     CDS             16017..17096
                     /locus_tag="ACHAY1_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8593450.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17093..17711
                     /locus_tag="ACHAY1_001448"
                     /pseudo
     CDS             17093..17711
                     /locus_tag="ACHAY1_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17821..18048)
                     /locus_tag="ACHAY1_001449"
     CDS             complement(17821..18048)
                     /locus_tag="ACHAY1_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8593451.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18151..20739)
                     /locus_tag="ACHAY1_001450"
     CDS             complement(18151..20739)
                     /locus_tag="ACHAY1_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8593452.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20759..21418)
                     /locus_tag="ACHAY1_001451"
     CDS             complement(20759..21418)
                     /locus_tag="ACHAY1_001451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8593453.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21467..21970)
                     /gene="trmL"
                     /locus_tag="ACHAY1_001452"
     CDS             complement(21467..21970)
                     /gene="trmL"
                     /locus_tag="ACHAY1_001452"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8593454.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22191..23057)
                     /locus_tag="ACHAY1_001453"
     CDS             complement(22191..23057)
                     /locus_tag="ACHAY1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8593455.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23166..23807
                     /locus_tag="ACHAY1_001454"
     CDS             23166..23807
                     /locus_tag="ACHAY1_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8593456.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23869..25230)
                     /gene="mgtE"
                     /locus_tag="ACHAY1_001455"
     CDS             complement(23869..25230)
                     /gene="mgtE"
                     /locus_tag="ACHAY1_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8593457.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25248..26147)
                     /locus_tag="ACHAY1_001456"
     CDS             complement(25248..26147)
                     /locus_tag="ACHAY1_001456"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8593458.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26149..26946)
                     /locus_tag="ACHAY1_001457"
     CDS             complement(26149..26946)
                     /locus_tag="ACHAY1_001457"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8593459.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26924..27628)
                     /locus_tag="ACHAY1_001458"
     CDS             complement(26924..27628)
                     /locus_tag="ACHAY1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8593460.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27759..28334
                     /locus_tag="ACHAY1_001459"
     CDS             27759..28334
                     /locus_tag="ACHAY1_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8593461.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28452..29129
                     /locus_tag="ACHAY1_001460"
     CDS             28452..29129
                     /locus_tag="ACHAY1_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8593462.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8585 a         6177 c         5243 g         9155 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg taaatacagt attatttcgg ttgcttgctg
       61 cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga ttcccgacgt
      121 ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc aataaagcaa
      181 taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca gaataagaat
      241 ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata
      301 tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa atcaacgtag
      361 accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct tctcttaata
      421 gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat gaaacagctg
      481 tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta agaattccga
      541 ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca acaataccag
      601 ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta cccatgacct
      661 tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt tgcggcacga
      721 tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa
      781 aagcggccaa cagagagacg gcagataacg ctaccattcg aatgatcaat tttggacgat
      841 ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct aattgggtca
      901 acatcgtcaa tatcccgact acacttgtgc taacagataa actagctgct acttttgttc
      961 caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt gataatagta
     1021 aaataagcga agttgttaaa ggtgtttttt cattactctt cattgatttc ccccatttcc
     1081 tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact attcgccaca
     1141 cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa atgaagacaa
     1201 ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg ttatatccgt
     1261 gaaacagagc ttctccacca cacgcataca acacagacga agggatgact ccaatcacga
     1321 tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag
     1381 aagatggaat aaccggtatc atatagccta acattacacc tatttttgga tgtttcattt
     1441 gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat tttaagcgat
     1501 gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg atgatcgcac
     1561 ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta ggaatacctg
     1621 gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg
     1681 aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat aattggatgt
     1741 ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg attcccaacg
     1801 agatcaacat caatcggatt attttctttt tcatatttcc tcctgaaaaa aatctatatt
     1861 cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat aggatctcag
     1921 tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct tcaagtttag
     1981 ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca ggagcgccat
     2041 caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact gctttgataa
     2101 ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt tgttcttcag
     2161 cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat acgccaaatt
     2221 cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct tttaactcag
     2281 caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg ttctttttta
     2341 tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc cactgctttg
     2401 acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat agatagaagt
     2461 ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga gactttacct
     2521 tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc gtccatgatt
     2581 ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc agtgaatact
     2641 tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga atttttgata
     2701 actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac ttcatcaact
     2761 gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt ttcgcttact
     2821 tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga gtttcacctc
     2881 ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta tagttacttt
     2941 gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta ctcgctttgc
     3001 tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt
     3061 tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga ctattgaata
     3121 tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg tcttcggtac
     3181 aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca agaattattt
     3241 ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt caacatggat
     3301 accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt tagctgctga
     3361 tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta atttttcatc
     3421 gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca cacggtaagt
     3481 tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa ctgtaccagt
     3541 tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac ctacagtagc
     3601 catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt ggattttttg
     3661 aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt ttgctttgtc
     3721 tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta atacaacagc
     3781 accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg caacttcaac
     3841 tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt cggcaacagg
     3901 ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc tctttttagc
     3961 cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg ttctatatct
     4021 ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat tctacagtga
     4081 tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt
     4141 ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa actttcgcaa
     4201 ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt ttcaatagaa
     4261 cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa
     4321 caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct tttgtgaatc
     4381 ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct ggagttgctt
     4441 tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg agacatacct
     4501 ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg ttttatctga
     4561 tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag tatagcaaat
     4621 ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa taaacgctaa
     4681 atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg
     4741 ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa
     4801 gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt
     4861 tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag
     4921 aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct
     4981 atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg
     5041 cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga
     5101 agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt
     5161 aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg
     5221 tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc
     5281 taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact
     5341 ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa
     5401 tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa
     5461 acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt
     5521 atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat
     5581 tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa
     5641 aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac
     5701 aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt
     5761 aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc
     5821 aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt
     5881 gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt
     5941 taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg
     6001 gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc
     6061 aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat
     6121 gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc
     6181 tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt
     6241 cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat
     6301 tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga
     6361 agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt
     6421 tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat
     6481 ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga
     6541 tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat
     6601 tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt
     6661 tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga
     6721 ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc
     6781 caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt
     6841 ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg attgttttga
     6901 tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt
     6961 cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa
     7021 attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta
     7081 aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt
     7141 cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt
     7201 ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc
     7261 ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt
     7321 ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga
     7381 ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca
     7441 tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct
     7501 cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac
     7561 ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact
     7621 aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa
     7681 ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca
     7741 ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag
     7801 accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga
     7861 ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct
     7921 tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca
     7981 ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc
     8041 aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa
     8101 tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt
     8161 ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc
     8221 atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct
     8281 actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct
     8341 ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt
     8401 tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc
     8461 ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt
     8521 gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa
     8581 aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat
     8641 tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt
     8701 gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa
     8761 tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag
     8821 gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt
     8881 gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata
     8941 ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac
     9001 gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg
     9061 gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt
     9121 gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga
     9181 aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc
     9241 ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata
     9301 agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga
     9361 tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt
     9421 catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt
     9481 ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc
     9541 gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc
     9601 gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac
     9661 ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt
     9721 ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta
     9781 tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga
     9841 ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga
     9901 tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc
     9961 ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa
    10021 agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt
    10081 atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc
    10141 ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt
    10201 ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca
    10261 ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca
    10321 cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa
    10381 agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg
    10441 aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc
    10501 aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa
    10561 ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa
    10621 catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat
    10681 ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg
    10741 gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac
    10801 cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc
    10861 tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag
    10921 ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt
    10981 tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc
    11041 gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta
    11101 tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata
    11161 tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc
    11221 tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca
    11281 acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct
    11341 tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt tgtgtttttc
    11401 ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga attaattttg
    11461 taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa agcttgctaa
    11521 ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa tagcaggagg
    11581 aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc ggaaatggct
    11641 ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac tatacacttc
    11701 attatagtga atacaaagga cacgatgctg agattgctga aactttggca aaagaaacat
    11761 tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt ttgatcatcg
    11821 taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg gaagtagaat
    11881 tcccagttgc ttatattcca gcaggttctg gaaatgattt tgctcgaggg gcaggccttc
    11941 caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca gaaaaagtcc
    12001 acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt aataatttcg
    12061 gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca aaaaagcgat
    12121 tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt gctttattca
    12181 ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt ttttcaaacg
    12241 catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg attgttccaa
    12301 ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta
    12361 aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg aaatatttcc
    12421 atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat ggtcaagaag
    12481 atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact aaacagttgt
    12541 tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt ttattcggaa
    12601 aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt taattctttg
    12661 cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg atcattgaaa
    12721 acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct gcaaatgttt
    12781 gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa tatgttggtg
    12841 tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca aacaccccac
    12901 cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt tctgagtttt
    12961 ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac aagaaagtat
    13021 ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac agaaccatta
    13081 gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag gttactttgc
    13141 gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc tctttgcgat
    13201 taaacgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac ccataagaca
    13261 agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat cccgcactta
    13321 gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct gcatcgattg
    13381 ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa
    13441 caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcgcc atctttccta
    13501 cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca cgattaaaat
    13561 aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact gtttccttgt
    13621 cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta atcaaggcga
    13681 ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg
    13741 caacaacagt gataattaca cctatcagta aattcgattt atttgcttta agcaatgtcc
    13801 aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt tcctaacgca
    13861 aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc caaatcagtg
    13921 tagaaataaa gtttatacaa actttcgctg atcaagttga cgagatttaa ttgaccaaca
    13981 atcggaatat gaagatcaat ccaatacttc acttgttccg accccatcat accagcaaaa
    14041 aagctcatcg caatcgtcac tgaaatcaag atgctatcct tttgcaccag attcattttt
    14101 ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac acaacgaccc cagagcggta
    14161 acaatcatca accattgcca acgttcacca aagtctactt gatagataaa acgaaagaca
    14221 gctaacataa tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa attagcgatt
    14281 gaaactaacg attgctttct aggaatcaag gacaagcgaa taccatttga cgattgattt
    14341 gcttgctgat cctgtacatt gcggaccccc cacatcgtcc cgtagagaat cgccattcca
    14401 atcagagtga agaaataaaa acttttgaaa ctcatattac gacctgcatg aacttcttga
    14461 aaagatactt cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt ctccacttct
    14521 gccactttac tcgaaattat accaatacgc tgtaaaaatt gatccatgac gtttttcatc
    14581 aacgtctgac tcattcctgc ttgacccaca tggagcgaaa cagatgaacc atcaatgaga
    14641 taaaaaccag ccaaatcatc tgcatttagc ttttcattcg cttctacttc cgatagttcc
    14701 tgtacttcaa ataatggttt atcattgact tcgacttgtt ctaagactgt ggtaaaattt
    14761 tccgaaagcg ctgtttgatc cgctttgatt acaattccag tattaatcgt ttcaagattg
    14821 ccaccttgat caatctcgcc aaaggccgcc ataaacatca atcctaaaag aattggaaag
    14881 gccaatgtcc aaaacaacat ggatttattt cgcagcagta tttttgtgcg atatttataa
    14941 aaatgccaaa acattatttc ccctcctcta gtctctcaaa tccttgccag taatctctaa
    15001 aaagacatca ttcaaggacg cttctttggt tgctattttt ccgtaactga tcttttcttc
    15061 ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt tcagtagttg ttacttggag
    15121 ctgacctttt ttataagcga ttccatcaga agctattgtt cgctgaatcg ctgacaacac
    15181 attttctggg agtaatggga tgtcgatcgt caaacgatcc gccgtccgta ccatatcttt
    15241 tagagattct ttggttcctt tagcaatcac ctgtccttga tccatgatca cgatctggtc
    15301 gcaaagaatt tctacttctt ccatgtaatg tgttgtatag acaatggttg ctccttgtcg
    15361 attcagctcc ttgatacttt ctaaaatttt attccgactt tgggggtcta cagctactgt
    15421 cggctcatct aaaaaaatta accgcggctt gtgggcaatc ccacatgcga tgttcaaccg
    15481 acgtttcaag ccaccactaa gttctcctgg acgaaacgaa acatattttt ctaatcccac
    15541 taattgaatc acctcttcaa tcaaggcttt ccttttctct ttttcggaaa catataaacc
    15601 gcaaaaataa tcgatatttt cttgcacact tagttcgtca aagaccgcaa cgtcttgagg
    15661 aacgacccca atcattgcct tgacagcata attttcagga gtcattgttg tcccgaacaa
    15721 tttaatggtt cctttgtcat aatttaacaa agaaagcata cagttgatgg cagtcgattt
    15781 cccactgccg tttggaccta acagacctaa gacttctcct tcttgtactt gcagatcaaa
    15841 atgattcaat gctacaaaat ctccatagcg tttgactaat ttatcaattt cgataatcat
    15901 catgaaaaac tccttccaaa tttcttgttg atctgactat aataaagtgc aagaaaagaa
    15961 actagtgacg attggctaaa ccacacatga catttgtcac aatgaaggag tctgctatgc
    16021 ataaatctcg gctactcaac gaaactagct ttctactggt tatctcattg cttttgctgg
    16081 taagtcaagg attcgttgct atgcaagtta tctatttatt gatcgccgtt ttattcactg
    16141 gtgtcagtta tttactgcct acaaagccaa attggatttt ccaagttatt ctatttcttt
    16201 tatgtgcctt tttacctgat cttgtcctgt ttctgccagc gacgatgcga gtgatcgccc
    16261 ctgaagaaag ccaccttgat tacttgtttt tgttaggttt tttgttgatt tcgacggttg
    16321 gtaatcatcc ccccatcaag gtcgcctttg tcgtaatttt gctgtttttt tccttttact
    16381 tacgaaaaaa agatcaacga acgaccgatc ttgagaaaaa gtacattgtc tccaaagata
    16441 ctcattggga aaaacaacaa gagttatcgg aacaaaataa agcattggcc ctgtcacaag
    16501 aaacacaatt agccttgcaa attgtggaag aacggaaccg aattgcccga gacatccatg
    16561 acaatgtcgg tcatctgctg tcaagtgcga ttttacaaat tggtgcgctt gaaatgatca
    16621 atcaagaacc aaaactaaaa gaaccattag aaaatttaag tgccaccgtt cacactggga
    16681 tggatcggat tcgtcaaagt gtccatgacc tccatgagac atcgatctct tttcaacact
    16741 ccttagagtt tctgatcgct gattttcctt cttctgttac gattgaagga aacttgtttg
    16801 aaggattgaa tgaaacgcag caaaactgct tcttaatggt catcaaagaa gccttaacca
    16861 acagtatcaa acatagtcat gccaaaaccg ttgttttaca ttttcgaacg ttgccggcct
    16921 tttatcgctt gcaaatcacg aatgatggca ccagcccaca aaaaaattct gctcccggta
    16981 ttggtttgaa aacgatgcgt caacgtgttc aagaacaaaa gggacaagtc catattttcc
    17041 aagaaaacca gcaatttcag ttaacgatca ttttaccaag ggagggacac caatgataac
    17101 agtagtcatt attgatgatg atccatttgt gacgaaatcg ttacaaacca ttttagaaag
    17161 cacgaaagag atccgtgtgt taggcattgg tcattgtgcc aaggatgcgc gaaacccatc
    17221 gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc gatgctgcca
    17281 aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca ttcaaagatg
    17341 aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc aaacagaact
    17401 tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta tttggtaatg
    17461 aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat agtcatgctt
    17521 atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca
    17581 ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat tacatcagcc
    17641 aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat ttttatcagc
    17701 atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc gcatcattca
    17761 ggtttttttc acatcttcta ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa
    17821 tcaaggattc gcagggctgc caggattcgt tggattgttc ggcgtgttag gcggaaaaat
    17881 aggcgccttg tctaacgttt tttctagttt tgattcttca cgatcgtctc gttctacatc
    17941 aggactttca ttcaatttga attctttttc catctcatct aactttttaa agtcattgcc
    18001 agattctttt gctaatttcg ctaatttatc ttcggtttct ttagccataa agtaacgccc
    18061 cccttttgat ttcagcttag catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg
    18121 atcattttta gctatttctc atctttaaaa tcaagcattt tttgccggta taaaatcgta
    18181 gggagaactg ttttccatac caatcatcgg atctactaac tggttatcga tcacttctgc
    18241 tgtaagaacg aaatctttag aaacagcagg ttctacctct tctttctttt cttctccaac
    18301 aaatgaagaa aacagatctg gttccactgt ttccattact tggtttttcg tttcattcgt
    18361 cccactgtca tcttctacag gtagcttttc ttttacaacc cgtggctctt ttgtatagtt
    18421 cccatcttca aatggatcat ttccgaccaa agaatcttca taagctgcta gtttggtccg
    18481 gagagtgata ttcattttat cattatccat gatccttgat ttcaacgtag tcatccggct
    18541 tgctgattca ttcgctacac aagtgacata ggcttgctct tcccctaaca aaatcaacag
    18601 ttccttactg cgagtcactg ccgtgtagag caaattacgc tgaagcatcc gctgatattg
    18661 atggaccatg ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat ggattgagca
    18721 acaataagaa agagtgattt tattccattc gtttctttta taagtgacct catttgcatc
    18781 aaactggata actaattcat ccactttatc ttcagaatct tttgccaaga tgatacccgt
    18841 gatttctccc atatctccat tgaatacatt caattcggga gtattgacta attggaggac
    18901 tttgtctcct atccgataaa cggtgtcgtt ccaatgtact tccttttttc caggtttccc
    18961 tggattgaat atctcctgca tcattttatt taacgcatcg atccctgcag gtccccgata
    19021 cattggtgct aatacttgga tatcttgtgc acggaatccc ttatttttcg ctttttccac
    19081 aattttagag atcaagggtt caatttgata agcatcactt cggaaatagg aacgatcttt
    19141 ttgattcttt gtgaaatctt ttggcagacg tccttctttg atctcatggg caagaggaat
    19201 aatactagaa ccatcacctt gccgataaat ctgattgagt tccattttgg gaatttgttc
    19261 gatttccaaa agatcatgga gtacttgacc aggaccgaca gagggaagct gatctttgtc
    19321 tcctactaag ataacttgca tattcgtcgg aatcgctttg aataatgtgt tagccagcca
    19381 tgtatcgacc atagacatct catcaacaat caaaagtccg ccttctaatt ctttcgtact
    19441 gactgaaggt gttttttccc gtccattcag ccccagaaga cgatggatcg tactggcagg
    19501 cagacctgtc gtttcgttca ttcgtttagc tgctcggcca gttggtgctg caagtagaat
    19561 aggaaaaatt tcttgggtat attttgaagg atccaattcc agtccgttca actctgcaaa
    19621 aagattgacg attccgttga tcacagtagt tttccccgta cctggtcctc ctgttaaaat
    19681 aaatagaggg gaacggattg cttcagcgat agcttgttct tgcgaaggtc catactgtat
    19741 gccgaatatt ttttcgatac gccgaatatt cttctgcact tctttttcag gataatggat
    19801 ttctttttta cgactgagaa ggcgctggat cgaattaccg atcccccatt cagaaaagta
    19861 caaactgttt tcgtaaagat tagtctcttc ttgttgtatc tttccttctt caacaagttg
    19921 gataattaca gtagcaacct gatcaggctg gatttcaatt gggcgacttg tttctaaaat
    19981 acggatcgtt tgctgaagca atgtctccgc cggtacgtat gtatctcctg tttgaactgc
    20041 atgctgaaaa atctgatgca agatagctgc tcgaattctt ctcggtgaat ctgctgcaat
    20101 accgatttgt tcggctagat tatctgcacg tttgaacccg attccttcta tgtcttcgac
    20161 tagctggtac ggattttctt cgatgatatc caaagactcg ttcttatagg cctggtagat
    20221 actgaaagct aactgactac cgaacccgta acgattcagt cccatgatca cttgatccat
    20281 cccgtggttt agccgaatcg tctcagcaag catttctctt tttggcttcg tcaaaccgga
    20341 gatttgtttt aataccgaag gatcagcaat gattttttcg atagcatctt ctcccagcaa
    20401 ttctacaatt ttttctgctg ttttcttccc gattcctggg aatttttcgc tggaaagata
    20461 cgctacgatc ccatttgcag ttgttggctt ttcttgctca tatctttcga gtttaagctg
    20521 ccttccgtat ttaggatgat ccactaattc tccataaaaa cgataagtct cattttcttg
    20581 aatatcgccg aaacttccgg tgactacaat ctctttttca cggtaatcag ctgtggtttc
    20641 actgatgtgt accaaaagca ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc
    20701 aactgtacca acgaaataag gcatttcttc tggctgcatc ggaagccgcc tccttcttct
    20761 atagtaaagg ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc catactttca
    20821 gttcatatgg caataatctg caaggctctt cagtctctaa aatcgtcaaa ctactattta
    20881 acaatccacc catttcacgc aactcactta gctcttttcc tgtcatccat tgaatggctg
    20941 cagttaaaga agctccatgt cctacgaata aaagtgggcc atctttatgt cgggcaaccg
    21001 catctgctac aacagtttcg atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag
    21061 gagaaggatc atacagatct aaatgattac gcaaattcgg cagattatcc ggatatttct
    21121 ggtacatttc atcgatcgac tgaccttcta aagtacccag ccctagttct cttagcttat
    21181 ctgtataaaa aatctctacc ggacgagcaa gctctttgtt gataccttct gctgtatcac
    21241 gtgctcgctt tgctgtactg gaataaattg ctttaaaagg aacatatttt acctttttcc
    21301 caaatgcttt gatttcttcg taactttgtg gcaaaagcgg agaatcgcca ttgctccctt
    21361 gaaaacgtct ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat agttccatac
    21421 gcagcaaact cctgtctttc ttttatttta ctctataata aactgtctaa tccagcttgt
    21481 cattttcata gtgatgcgtc aattctaaat tagggaatcc ttgctggcga agtgcttcat
    21541 acactatcat tgcagctgta ttcgataaat tcaatgagcg gacatgctca tcattcatag
    21601 ggatgcgtaa acacttttct tcgttttcac gcataaatgt ttctggcaaa ccggttgttt
    21661 cttttccaaa taaaaagaag tgctcttctc catcagcaaa attttcgtca ctatatactt
    21721 tatgggcaaa ttttgagatc aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt
    21781 ctaaattact gtggtaagta atatttacat cattccaata atccaaccct gcccgtttta
    21841 gatgtttgtc atcagttgaa aaacctagtg gctcgattaa atgaagcgga gtattcgtgg
    21901 ctgcacacgt acgggcaata tttccagtgt ttgcagggat taatggttca aataaaacga
    21961 tatgattcat caggatcatt cctctcatat ttatgttcgt ttctattttc tgacaatttg
    22021 atttttgcat aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc gttacgttag
    22081 tgaagctctt ctcactaact tttatcagaa caggaacctg ataaaaacca atgaaaaaac
    22141 aacttgttgc taatataaca ccctcaactt ttttttgcaa cctctactca ttaaaaactg
    22201 ctagaaaaac gttttctctt agttcctttt aataattcta tgtcaattgt aatcttcttc
    22261 aaaggattct tatataagtc tgctttgatt cgctcttcta cctgccattc taaaggtgac
    22321 atggccaaaa gatctttttg attttcctct ggtatctcaa attcataggt tacacgctga
    22381 cgttcagtta atttcattgc ttcagcaaac ttggctacta cacgttcatt ggaataactc
    22441 tgtttttcag gttgattcgg ataaaaagcc tgacgtagtt cctttaaata attttcttgt
    22501 ggcaccacct tcaacacttc tccatcttct gagagtacac gttggaattc ttggtaatga
    22561 gaaggcgaaa agatatttaa aaccgcgttg aatgtttgat cagcaaacgg caaattggtc
    22621 aaatcagcta cacaccaaaa tgcttcggca gattgttctg tagctgcgta gactccttct
    22681 tttgaaatat caaatccaac agcattttga atctttttca attttgaaag ttcattcaag
    22741 aagctacctt ctccgcagcc aacatctaat agcgagactt cttcaggtaa atgctgcgca
    22801 atagtctcaa taacgggctg atacatgcca gattggatca ttctacgcct tggaacgaac
    22861 attccttgat cgtattctgt tttaaccgca tgggataaaa aataaagtgt tccttttttg
    22921 gaaaggtcaa aacggtgatt atttttgcat atcaagccat tatcagactt ttttacttct
    22981 tcttggcaaa ccggacagcg gaacagctga tgattttctg ccaaaaactg gcgtcctcgg
    23041 tcaatttttt ttaacatagt tccccctcat ttctgtttta tcttaccgtt agatgctcac
    23101 tttggcaact gctaaaaata tgatacaatg cctagaagaa agtgaaagat aaaggagaag
    23161 attagatgat caaagaattt tgcgctgaaa actacacatc tattcctcta gcaattgcta
    23221 gaggagccga tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca accccgagta
    23281 ctggtgtgat cgaagaaacg ttaagttatg ctggggaaaa agatatcccc gtaatgacga
    23341 ttattcgtcc tagagggggc gacttcgtct ataacgatat cgaattgaaa atcatgcata
    23401 ctgatttgat tgaagcaaag aaattaggga cggatggcgt agtatttggt tgtcttactc
    23461 cttcaggctg gctagacgaa gaggccttag aactattgat cgaaacagca gaagggttgc
    23521 agatcacctt ccatatggct tttgacagta ttcctgaaga tcggcaattc gaagcaatcg
    23581 attggctggt agaacatggt gttgaccgta tcttgactca tggcggtcct gctgacaaaa
    23641 ctattgtaga aaatttccca cggcttaaag aattgattgc ctatacagat ggccgcttaa
    23701 tcattttgcc tggtggtggt gtcactgcag agaatgcagc acatgtagct aaagaattga
    23761 acgtaagcga agtacacgga accaaaatcg tggatcttca gttataatct tataaacaaa
    23821 aaaacatttg caacaggcat aatgcttatt gcaaatgttt ttgttttttt acataaaata
    23881 gcccataaat aaagaagcaa tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt
    23941 cgtgataaag ggaccgcttg ctactgctgg atcaaatcct agacgatcca ttagcatcgg
    24001 gattaaacta cctgcaagat tcgccactgt gattgcaaat agcatagcca tccctatgac
    24061 aaaccccaag ataaaattat gtttccaaaa acctacgatt agaaaaatcg tgatccccgt
    24121 aacagctcct gtgacaacgc cggtcaagac ttcagccaaa acaagtcgaa caaatccatg
    24181 atctttctca tcagaaatcg caagtcgacg aacagccaca gctagcgact gggttccagc
    24241 attaccggca gttcctgtaa tcagcgaaat gaagactgct aaaatactcg cttcacttac
    24301 caagtcttca tagtgactga ttaaagaagc ggtcgacata cctaaaaaca acagagtgat
    24361 cagccaaggc aaacgtcgag acgctgcctt tactggattt tcattaattt cttccacatt
    24421 gaccccggca agtcctgaat aatcgctagc agcttcatca tcaataacgt cgatgatatc
    24481 atcgactgtg acgattccta acaaatgatc atcataatcg gtaacaggaa gagctaagaa
    24541 gtcataatct ctaaatgttt gtgccacatc ttcttggtcg tcgcctacat gaactgaaag
    24601 cactcgttcg ctcatcagat cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg
    24661 cagagaaata acccctacta gctgaccttc ttgattaacg acatagatgt aatagattgt
    24721 ttcagctaca tctgcttcgt tcttcagcac gtacatcgca gagcgaacag tttgattggc
    24781 tacgatcgaa acaaattcag ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag
    24841 aagttccttg atttcactgg catcgtctgc cgacatcaaa cttaaatatt tagcaatttg
    24901 ctttttatcc agcgtattaa gcaagtcaac agcattatct gtatacatct cagaaagcat
    24961 atcagctgca tagctaggac gcatttcatc taggtattct ttcatatgtt cgttgtcttc
    25021 ttcaatcaca tcgaacatat ccgctaattc tttaggcgac agataggaat agatcagctg
    25081 cctgttctct tctgtaagag attggtagaa ttgcccctgc tcatagatat gcatctctaa
    25141 aaaactgttt ctgaatgcct gaatattatt ttcccttaaa tcttgagtca actgggcaaa
    25201 tctttcctct aattcttgat tttcatccaa accaatctca cctcgcctta ttcttcaaac
    25261 cgtctcacaa ctgcctccat gtctgcggct aaaggctgct tgaactccag caactcttct
    25321 gtaaatggat ggtaaaaacg cagatcataa caatgaaggg cttgtctttt tattcccaga
    25381 tccattcgcc cgccatacat atcatcaccg agcagggcac agccaattgc ggaaaaatga
    25441 actcttattt gatgtgtcct tcctgtatgc agctggatat caactaacgc gatttctgtt
    25501 agtcgcttct ctatccagta ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc
    25561 ttcaatagcg aagaataatc tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa
    25621 tctgaaagcc ttccactgac taaggcctga tattttttca caaacttctt ttcacgaagt
    25681 tgcatatcta attttgcgtg cgcaaatcca tgctttgcaa aaagcatcaa gccagatgta
    25741 tcccgatcta gtcgcgtcac cacatggatt acttggtttt cgtaaccttg ttttttataa
    25801 taagccttta cacgattagc cattgtccca tttggatggt attgggcagg aatagacgaa
    25861 acgcctgctg gtttatttac taccagaacg tgttcatctt catacacgat atcgatcggt
    25921 gtttcatcca aaagtacggt ttcatgttct ccttcagcag gaatcacaat ggttacctta
    25981 tcatccttag ctagagaaaa caagacattt tctacttgat cattaacttt gatttgaccg
    26041 ccttgaaatt ttattttcgc taacagaccc tttgagatcc ctttttcttt taaaaaatat
    26101 tttacttgct gagaatgatc tttctcataa tcccatgtaa actgcatatc aatcctcact
    26161 aataaaggca tctttcacac gatgccaaaa atgcatatgt cgataagaag caaaatggat
    26221 ccgttcttct gctattcgat aataaattga gcggatattt gcttttgcga cattcaattg
    26281 atcaaccgtt acaagataat cttctgtatt ttccaatttt atttctagcc attcatcatg
    26341 ggcaatcacg atcggagaac ccaaagtacg aaacactcga ttattcagtg aagcaatctc
    26401 tgccaactga aaggcattga tactaggatg gatcacagca ccgccgacac ttttattata
    26461 tgctgtcgaa ccagtaggcg tagaaatcgt caatccatct cctctaaaac tttcaaacag
    26521 ctcgtttttg atgtatacat cggctaccat cgtacgattg gcccgtttaa tcgttgattc
    26581 attcagtgct aaaaaatgct ggtccggtgt ttcatccaaa taactgatcc gaacatccag
    26641 caagggataa ctaacacttt gttcccggtt cgttcgtaag ctgtctacta gttcttccag
    26701 ctcgtaatcc cgccaatccg tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg
    26761 cacatcattc agacaatgac tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc
    26821 gactgagatg accaattctg gctcatgatc atctcgttga atccctgctt gttctaaaag
    26881 tgcgttcaaa cgttctgtca cttctatagt ctttgcttct ttattatgaa cgatcgctac
    26941 tttcatcccc tgcctcctct ctttcgcttt ctttcttcaa aagcgttcga tagtagacat
    27001 catctgattt tcctcttcca tgggagaata accgctgtgc ttcttggatt tcttcacgga
    27061 tactggacat ctcttcatcc aactgatagg cagcttctgc cgcacgctga agtcgttcac
    27121 tcatttcttc tggaaaaacg ccttgatatt tataattcag cgaatgctca atcgttgccc
    27181 agaaattcat cgcaagagtc cgaatctgga tttccgccaa aattttcttc tcaccgctga
    27241 tcagctgtac aggatactcg atcaccagat gataagaacg gtagccactt gcttttttat
    27301 ttgtgatgta atctcgttct tggagaactt tcatgtcttt tcttttacgg atgatctcaa
    27361 caacttgatg gatatcttct acaaactgac acatgatcct taacccggca atatcagaca
    27421 tttcttcttc taaacgtccc attggaatat gccgtaattg tgattttgtt acaatactat
    27481 cgactggctt gactctccca gtgacaaatt caatcgggac atgtcgattt tgttcacgga
    27541 actgtttgcg gatcccccgc agtttcactt tcaattctga tacagtttgt tcatatggtg
    27601 ctaaaaatgc ttcccaatct cgttccatag ctgatacctc tacttcatta acattcattg
    27661 tttattctac catatatgca aagttttttc gtactcttga tttatgcatt gaccaaaaaa
    27721 agaaaattag gcaaaataga caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg
    27781 aatataaatc tatgctgaca aagcaagagt acgagatatt catcgctcac tatcaattaa
    27841 cagataaaga tttccgtgta cagactaata tttatttcga taccgttgat gaacagctga
    27901 agaaacaaaa ttgtggccta cggatccgtt tcgttggtca tcaagctgaa tatacattaa
    27961 aaacacctgc tgaaaaaggg cgtctcgaaa caacggatac ctttataaca gaagaagcag
    28021 aagcatttat cttcgaaaaa cgtctgcctc gttcaggcgc agtttttcaa aaactttctg
    28081 atttgaatat cgatcctgct tcattaatcc aaaccggaaa gcttacaaca aaacgagctg
    28141 aattccctat tgaagaagga cttttagcta ttgatgaaag ctggggagac aatctacacg
    28201 attttgaact ggaactagag gtaggggatg catctgtggg aaaaattgct tttataaatt
    28261 ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg
    28321 aagctaaaaa ttagaaacct ttcattttca caaggcactt gagttcattt ttttactttt
    28381 ttctgttaag ttataggatg tgaaagaatc tagtcactag ttgtatagaa tctattttgg
    28441 gggagattca tatgatcgaa atctatttat ttgttaatcc tttaggggaa tactgtttcg
    28501 aaatggaaca acagttatta caatttatcg aagaagagta cgggacacca tcaaaagaaa
    28561 agatgcaatt tcgctttctt cctttggtca atttgcagac aattggggat gtcatgcaac
    28621 aaaaaggaat ttctcagaac aatcttgagg aacgtaatca cttattctca atgacttatt
    28681 ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt
    28741 tgatcaaatt gcaagaagca gtcggctgta atgacatacc atattctcaa gaacttgctg
    28801 aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg
    28861 acttcgttaa agaagtattc ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa
    28921 aacacccttc tgcagtcgtt tacaattatg cctgcgaccg tgattttggt gttttagtag
    28981 aagtaaaagg tacaagtagc ctttggagaa tcacagagct gtgtaaaaca acagataact
    29041 catatcaagt ttttaatggt gaacatttac atacaaatga ttctcagttc cattcaagaa
    29101 ctagccattt acacttgctt tctaattaaa aaaggctctt tgtcaaatga tgttggtaaa
//
LOCUS       DAYYCM010000032        28910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000032 DAYYCM010000000
VERSION     DAYYCM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.033"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1622
                     /locus_tag="ACHAY1_001461"
     CDS             105..1622
                     /locus_tag="ACHAY1_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8593463.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1594..2883
                     /locus_tag="ACHAY1_001462"
     CDS             1594..2883
                     /locus_tag="ACHAY1_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593464.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3058..3954
                     /locus_tag="ACHAY1_001463"
     CDS             3058..3954
                     /locus_tag="ACHAY1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593465.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3968..4831
                     /locus_tag="ACHAY1_001464"
     CDS             3968..4831
                     /locus_tag="ACHAY1_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593466.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4837..5400
                     /locus_tag="ACHAY1_001465"
     CDS             4837..5400
                     /locus_tag="ACHAY1_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8593467.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5422..6933
                     /locus_tag="ACHAY1_001466"
     CDS             5422..6933
                     /locus_tag="ACHAY1_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8593468.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6945..7904
                     /locus_tag="ACHAY1_001467"
     CDS             6945..7904
                     /locus_tag="ACHAY1_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8593469.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7990..9069
                     /locus_tag="ACHAY1_001468"
     CDS             7990..9069
                     /locus_tag="ACHAY1_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8593470.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9321..10721)
                     /locus_tag="ACHAY1_001469"
     CDS             complement(9321..10721)
                     /locus_tag="ACHAY1_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8593471.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10939..12600
                     /locus_tag="ACHAY1_001470"
     CDS             10939..12600
                     /locus_tag="ACHAY1_001470"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8593472.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12557..13255
                     /locus_tag="ACHAY1_001471"
     CDS             12557..13255
                     /locus_tag="ACHAY1_001471"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8593473.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13271..14695
                     /gene="araA"
                     /locus_tag="ACHAY1_001472"
     CDS             13271..14695
                     /gene="araA"
                     /locus_tag="ACHAY1_001472"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8593474.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            15002..16162
                     /locus_tag="ACHAY1_001473"
     CDS             15002..16162
                     /locus_tag="ACHAY1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8593475.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(16139..16279)
                     /locus_tag="ACHAY1_001474"
     CDS             complement(16139..16279)
                     /locus_tag="ACHAY1_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8593476.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16280..18139
                     /locus_tag="ACHAY1_001475"
     CDS             16280..18139
                     /locus_tag="ACHAY1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8593477.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18249..18683
                     /locus_tag="ACHAY1_001476"
     CDS             18249..18683
                     /locus_tag="ACHAY1_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593478.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18942..19103)
                     /locus_tag="ACHAY1_001477"
     CDS             complement(18942..19103)
                     /locus_tag="ACHAY1_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593479.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19122..20144)
                     /locus_tag="ACHAY1_001478"
     CDS             complement(19122..20144)
                     /locus_tag="ACHAY1_001478"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8593480.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20272..20625
                     /locus_tag="ACHAY1_001479"
     CDS             20272..20625
                     /locus_tag="ACHAY1_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8593481.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20637..21803
                     /locus_tag="ACHAY1_001480"
     CDS             20637..21803
                     /locus_tag="ACHAY1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8593482.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21895..22515)
                     /locus_tag="ACHAY1_001481"
     CDS             complement(21895..22515)
                     /locus_tag="ACHAY1_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8593483.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22707..23003)
                     /locus_tag="ACHAY1_001482"
     CDS             complement(22707..23003)
                     /locus_tag="ACHAY1_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8593484.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23207..23857
                     /locus_tag="ACHAY1_001483"
     CDS             23207..23857
                     /locus_tag="ACHAY1_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8593485.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23873..25600
                     /locus_tag="ACHAY1_001484"
     CDS             23873..25600
                     /locus_tag="ACHAY1_001484"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593486.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25597..27489
                     /locus_tag="ACHAY1_001485"
     CDS             25597..27489
                     /locus_tag="ACHAY1_001485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593487.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(28049..28597)
                     /gene="aac(6')"
                     /locus_tag="ACHAY1_001486"
     CDS             complement(28049..28597)
                     /gene="aac(6')"
                     /locus_tag="ACHAY1_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8593488.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         9345 a         5040 c         6016 g         8509 t
ORIGIN      
        1 tggttaatta aaatcgataa aaaataagta agtttaacat ttatattatt gacaacgctt
       61 tcaacgaggt gtattattca tgtataaatt aaaggaggtc agttatggaa gccacaatgt
      121 tgattagtag tgtcaagcgt gtaggcaaag ttgatccgcg gttgtacagt tcatttattg
      181 aacatatggg acgagcagta tatgaaggga tttatcagcc agatcatcca tcagctaata
      241 aaagtggatt tcgccaagac gtcattcact tggtaaaaga attgaacatt cccatgatta
      301 gatatcctgg tggaaatttt gtgtcaggat ttcgttggga agacagtatt gggcccatag
      361 caaatagacc tagaaggttg gatttagctt ggcggacaat tgaaacgaat gaagttggga
      421 ttcatgaatt ttataactgg tgtcagcatg tgggggccga aatcaatatg gctgtcaatc
      481 tgggtactcg tggagtagat gcagctcgta atttagtcga atattgtaat tttcctaaag
      541 gaacttattg gagtgattta aggcgttcga atggtcaaga gagccctttt ggaattaaga
      601 catggtgttt agggaatgaa atggacggtc cttggcagat tggacataaa acagcggaag
      661 aatatggacg gttagcagag gaaacggcta aggcgatgaa actcgtagat gattcaattg
      721 aggtggtttt atgcggaagt tcgaatcggc aaatgcctac atttggtgat tgggagttaa
      781 cagttcttga tcaagcgtac gatcaaatag attatttgtc tctgcatcaa tattatggca
      841 accaaaaaaa tgatctttct cattatcttg cacgttcctt agacatggat cggttcattt
      901 ctgaagtgac agcgatgtgc gatacagtca aggcgaagaa acatagtaag aagcagatca
      961 acctgtcctt tgatgaatgg aatatttggt atcattcagc ggaacaagat gagcaagcag
     1021 cgccttggca gattgctcct cctttactag aagataacta taattttgaa gatgcattac
     1081 tattaggctg tctattaatc actttattga aacatgcaga tagagtaaag attgcttgtc
     1141 ttgctcaatt ggtaaatgtt attgcaccaa tcaggacaga aacaaacgga gaagcttgga
     1201 gacagacgat ctattatcca tttatgcaag ctgctgtgta tggacaagga gaggtgttaa
     1261 gcccacaaat tcgttcagca aactatgcga cggaagattt tgaagaagtg ccgtatttag
     1321 aaagtatagc cgtaataaat gacaaagaac ttgtcatatt tgcagttaac cgtggagaag
     1381 aagaaatgaa tctaactgtt cacttgtctg aattatcatt agagggagtg attgattttt
     1441 ctgaaatgag tggatttgat acaaaagcaa ctaatcttcc taaaagtgaa caagtaaaac
     1501 caagtcattc aacaaatgta aaagctgaag atgggaaaat caattgttta ttgaaaccgc
     1561 tatcttggaa tgtggtcaga tgtcgtttat cagatgaagg aggaacaaaa aatgaaaggt
     1621 aaaaaatggt taattggctc tttgttggta gctgcaacag gtattttagg agcttgcggt
     1681 ggcggaagtt cagcagaatc tgattctgat aaagaagtga ctttgacttt ttggaatggg
     1741 tttactgcat ctgatggtga aatattgcaa gaaatcgtta atgaatttaa taaaacaaat
     1801 gataaaaaca tcaaaattga aatggatatc atgacttggg ccaatttgaa tgaaaaactt
     1861 cctacagcga tttcttctaa aaaagcacct gactttattg cattaaatta tccagatttt
     1921 gcacagtatg tagaaaacgg agcggtacaa ccattagacg atttctggga atatgatgga
     1981 gtagataaat ctgattttaa agaaacggct attgaattag gtgaagtaga aggtaaacag
     2041 tattttgtcc ccatgcaggt acaaggcatg tatatgtatt ggaataatga tttgtttaaa
     2101 caagctggat tagatactga acaaccacct actacttggg aagaattgac agaaatggca
     2161 ccaaaattaa ctgatagtag taaaaatgta tcaggttttg tcttcaataa ggatggaact
     2221 gcaccgttat ataactggat gttagcaaat ggcggtaaat tagtgaacga tgattatact
     2281 aagtctgaat ttgcgtctcc tgagaacctt aaaacgttaa aagcgatcca aaaaatgatc
     2341 catgtagaca agactggtcc agaatcgatt tctggcgccg aaatggacaa tttaatgaat
     2401 gctggtcaac tagccattga aatcaatggg ccatggttaa ataacggatt gaaagcaaac
     2461 gagattgatt ataatgtgac taccttacca caagggagtg attcagacaa aaaaacagct
     2521 attttagatg gcgtaggata tgccattccg gcaagtacag ataataataa aaaagaagct
     2581 gtctatgagt ttttgaaata ttggaataca actgaaattg gtaaaaaatg gagtatcgaa
     2641 aatggttttc ctgcctactt ggattcagta gcaaaagatg aagaagtaaa aaatgatcct
     2701 attgtatctg aattatcgaa acaaatggac tatgcagagc catatttacc aggttttacc
     2761 caaatctctg cgatcaataa cgatatcatc aatccactga ttgaaaaatt attagcagga
     2821 gaagatcctg agacactcat gaacgatgca gatgatgcca tcaatcaatt actgcaaaaa
     2881 tagtacagaa ctttatacag gatagactgt tggcaagata cagatatctg aatgatcgac
     2941 ctagtatttg cagataggtg ttattgcaaa aatcacagcg attgttcgga tatcttattc
     3001 agattcgttt aaaccaagat tgtatatttt atcaaaatga aagatgaggt ggactatatg
     3061 aacggattca ccaaatattg gaatcgtcct gatcgtgctg cgtatttgtt tttaactcca
     3121 tcttttttag ttttattatt atttactgtc ataccgcttt taggaacatt tgctattagt
     3181 tttacagatt tgaatatttt ctttacatca agaaattttg taagttttga aaatttcaca
     3241 cgtatattta gtgatgaacg agcagttaat tcgttgattc attctttata tttcaccgta
     3301 ctcgaaacgc caacacaaat tatcgttggt ttattagcag cagtagcatt atctaaaaat
     3361 accagattca atcgtttttg tcgttcgaca ttttacattc cagttatttg ttcattgacg
     3421 tcaattgcga ttattttttc tatgttgctt gatccgaatg taggggcgat tccttacttg
     3481 ttttcacaag tcggcttgcc gatccctcaa ttttttcgcg atccaaccct tgctatgccg
     3541 acggtagcca tcatgactgt ttggaagaat tttggtgtta ctatgacgat tctcttgact
     3601 gctattcagg gaatctctcc ctctttgtat gaatctgctc aaatggatgg tgcaacaaat
     3661 cgacagcagt tttttaacat tacgctacct cagatcgttc cctccttagg tttttgtatt
     3721 ttaacgaatc tgattggttc gatgcaagtg tttgaccaag tatatgttgc aaccgctggt
     3781 ggcccacaat ttaagacaga aacagctgtt caatatattt acagtcgagg atttacagca
     3841 ccttatgaat tgggctatgc atcagcactg tcttcggttt tgtttatcat tgttgcaact
     3901 atagctatct ccactaatgt gtacatgtca cggaaagaaa aacaaatgaa ataggaggga
     3961 aacctgtatg gcaccagaaa gctcgattac agttactgtg aatagtcgaa aagcagttag
     4021 tggtaaaaaa atcattcgaa cgattagtat gctagttata ttgttggtta ttgcgtttcc
     4081 ttttttgtgg ctgattattt cttcttttaa acacgaaaaa gacattatct catttccgcc
     4141 tagaatattt gcagatagct ataccctaga taactatatt aaggtttgga caacgatccc
     4201 tttattagat tacatcaaaa atacagtgat ttttgctgga gggacagtga tcacttctgt
     4261 attctttgat tctcttgcgg ggtatgcatt tgctcgtatg cgtttcaaag gaaaatcagt
     4321 tttgttctat ttcgttttgc tgacaatgat gatcccattc caagtcttca tgattcctct
     4381 atttattgaa gtcaatttgc ttggcatgct tgatacttat gcaggtttga tcattcctcg
     4441 tatgacaact gcatttggta tcttcatgat gcgttccttt tttattactt taccagattc
     4501 tttggaagag gcagcacgca ttgatggatt aagtgagttt aatatttttt tgaaaatcat
     4561 gttgccattg agcaaaccta ctcttttatc attagggatt ttcacgctga tgaatagttg
     4621 gaatgacttg ctttatccat tgatattgac aagtagttca aaaatgagaa ctttaccagc
     4681 agggttagct ttatttacag ggcagaacat ttctttttat ggacctgtaa tggctggaac
     4741 ggttatttca atgctgccat tactagtggt ttatattttt gcacagaaat attttgttca
     4801 aggaacagct atgtctggaa tgaaagagtg aggaagatga aaatagttga gactaaactt
     4861 tatggacagt tgattgtggt ttctgattac atcttattag gtattctctg ggtaatcgtg
     4921 tgtttgcctg ttattactat cgtgcctgct acatgtgctg ttttttatgt aatggcgcaa
     4981 tggaaaggtg agggatcagg aaatatcatt tattttttct ttcaaggaat aaagcaacat
     5041 tttggtaaga acttgttcct tagtctgtta acgatcatta tttattttct tgttaatcaa
     5101 ttattagaag aacagacaat cgtactttca attagtggat atgtagtttt acttatttat
     5161 tccatgtttc ttttatcctg gatatatgtg atttctgaaa acaaagatat cacattttta
     5221 caaatttttg aacaaagtgc atggaaagta ttttttgatt ttttgagaaa tattctttgt
     5281 tgcgtattgt tattaggatt tgtactgctc atatttcttt tccctccttt tatttttatc
     5341 tttgctggtg gcgtatggaa acttgtatct attatcttga ctataggaaa tgaaaggtag
     5401 ggaaagctgt tggacgaaga agtgaaagta aaaatcagag aggattttac ggtaggagag
     5461 atcagttctc ggctgtttgg ctcatttgtt gagcatatgg gaagtgtcgt ctatacaggg
     5521 atttatgaac cgggccatga aacagctgat ggtgacgggt ttagacaaga tgtcctctct
     5581 tatgtaaagg aattagaatt aggtgtcatt cgctatcctg gaggaaactt tacatctggt
     5641 tatgattggg tggatactgt aggtaggaaa gaacaacgac caaaaaaaat tgatttagca
     5701 tggcgaggta tagaaccaaa ttcttttgga ctccacgaat tttttaaatg gatcagtcat
     5761 gtacaagcga aaccgatatt cacggtgaat ttaggaacaa aaggaattga tgaagcaaaa
     5821 aatttagtag aatattgtaa ttttccaaaa ggaaccttct ggagtgattt aagaataaaa
     5881 aatgggcaaa aaaagccttt tggcgttaaa ttatggtgtt tagggaatga gctagacggt
     5941 ccatggcaag tgggggctaa gagtgcttat gaatatggta ggttagcaaa tgaagcctca
     6001 aaagtcatga agttagtgga tgattcaatt gaaacaatct tagttggaag ctctacgcca
     6061 cgtttaccat cgtaccctga atgggatcgt atcgcattag aagaagctta tgagaatgta
     6121 gattatttag ctttgcataa ctatattgat aagtatgatg aagaagactt gactaaacct
     6181 cctaaaaatg agcgtgatga tacagcgaca taccttgcaa aagcttatcg attcgatcgt
     6241 caaattgaag aaatcgttgc tacttgtgat tatgtaaaag gatatttgag aagtgagaaa
     6301 acaatgtatc tagcgtttga tgaatggaat gtacatgccc aaccggaaaa aagacatagg
     6361 gattttgaag tcggtagtcc catcgattgg tgttacttta ctatggaaga tacattatta
     6421 tttggttcat tagggctggc tattttacga cacgcagatc gcgtaaagat tagttgtcaa
     6481 tcattgttag taaacacaat acctttaata ctgacagatg ctaaaggaca agcatggagg
     6541 aatccaactt attatgtgat gcaacactta tcgaaatatg ctaaaggtca agtattgaaa
     6601 caacaaatca tttgccctgt ttatgattgt gaaaagataa aaaatgttcc ttatgtggac
     6661 agtgtgatcg tagataacgg caatcagctg actattttct tgatcaatcg cacaaatgaa
     6721 caacaaaaga taacgattga acatgatttc aatttgctag atcaaggaga gcactacaca
     6781 ttaaccagta gcgatctgtt ggacaaaaat acacgtgaag aaccagaaaa aataaaacct
     6841 gaaataaact atttttcaat agaaaaacat gacgaattaa atgtagggat agaaaaatac
     6901 tcttggaatg taattagaat aaagaaaatg tgaggaaatt tgttatgatg aagacacttt
     6961 ataataatcc tatcgttcgg caacgggcag atccttgggt atataaacat acagatggtt
     7021 attattattt tacaggatcg gttccaggct accaagtgat tgaattaaga agggcaaagt
     7081 cactgaatga attagaacat gctgcaagcc tgattgtatg gcaggctcat gaagaaggac
     7141 cgatgagtga attgatctgg gcacctgaag tccattatat caatggaaaa tggtatattt
     7201 actttgctgc aagcaatcat aaaactatac gtgatgaatc tcatcatcac cgcatgttcg
     7261 tacttgaaaa tcagcatata gatccaatga atacagattg gatagaaaag ggacaaataa
     7321 aaacgaaaag ggaaactttc agtttagacg gaacaacttt ctcttctcaa ggaaagctat
     7381 attatgtgtg ggcccaacaa gatcctgaaa ttactgggaa ttctaatctt tatctatccg
     7441 aaatggataa tccttggacg ttaaagggga agcagatcct cctttctata cctgagtatg
     7501 aatgggaaaa acggggtttt tcagtaaatg aaggtccagc agtccttact cgcaatggga
     7561 aaatattcat tacgtattct ggtagtgcaa cagatgaaaa ctatgccatg ggcctgctgt
     7621 gggctgatga gcacaagaat cttttattaa aagaatcatg gcataaatta tcggagccag
     7681 tattcgtttc ttctaaaaaa aataagcaat ttggtcctgg tcacaactcg tttacggttt
     7741 cagaagacgg gtgctttgat attttgatct atcatgctcg accagagaag aaccaaaaag
     7801 gggatccgtt agacaatcct aatcggcacg cgaacgcgca atgctttacg tggaatgagc
     7861 aaggatttcc agaatttggt gaacctgttc cgtatgcctt atgagcacaa gaaaccttga
     7921 tttttgcttg aataaacctt gcagactcgt tatgatatat gtacgtacaa ataaaaaggg
     7981 gcaaggcaaa tggcaaaata tcaagatatt gcagaagaga taaaacaaaa gatccttcgt
     8041 gaggaatacg aagtaaatac aaccattccc tcagaattca agctgcaaga aatatatgac
     8101 gtcagcagac atacgattcg gcaagcaatc gctgtgttgg taaatgaggg ctatctgcgt
     8161 aaagaaaaag ggtcgggcac atatgtagat gacagctata agaaagaaaa aacaccattg
     8221 aacagaacaa aaacgatcgg tgtcatcacc acatatctct ctgattatat ttttccgtcg
     8281 attatccgag gaatcgaaaa tgaattacga gaatctgggt attcattgct tttagccagt
     8341 acgaataacg atcacgaatt ggagaaagag tgtttgaaga aaatgattgc ttatggggta
     8401 gatggtttga tcgttgagcc tacgaaaagt aatcagtata acccaaactt agcactttat
     8461 gttgctttga gagaacaagg cattccgatg gtgatgatca atgctgctta tgaagagtta
     8521 aatgcctctt atatttgttt agatgatgtg aaagtcggtt ttatggcagc agaatatttg
     8581 ataaaaaaag gacatcagcg tctgttgttc atcacaaaaa tcgacgatct tcaaggaaaa
     8641 tacaggatga aaggttttat cagcgcgtgt gaacagtata atgtgcgttt tttgacggaa
     8701 gatatcatta ctttcaccac ggaaacacga gaaacaatcg ctgaaaaaac agtacaacgg
     8761 ttgaaagaag aacaagcttt agatggcatc atttgctata acgatcaaat agctagttta
     8821 atgctagata agttgacaga aaatggctat catattccag atgacctatc gatcgtagga
     8881 acagacgatt cttctttaag ccatttaggg aataaaaaat tgacaacatt gacgcatcca
     8941 aaagaaaagt tgggacaaga tgccgcacgg tggatcgtca caacgattga aaaaggacag
     9001 gcacagccaa atattctcta tgaacctgta ctagttgaac gggaaacagt gaaagaaaac
     9061 gaaaaataag agacgggttt ttaccgcctc ttattgctcc tacggttact cgtcgcaatg
     9121 tttgtgagag cttttgtcat cgctcaaggc tgaacgtttc tttcaccacc tctccactaa
     9181 acggtgttct cctaccaact ggtcacaagt cacaatattc atcaaacatg agaagctgtt
     9241 ttttgaaatt gttccttgtg actggaaact aagcggttcc ttcttatttc ttgaagctga
     9301 ccacttctgt cacaacctct ttaagaatga tggatagaaa tacgtttagg ataagttttt
     9361 ttagatgcac tttgttcgat ttcctctaaa gatttcccac gagtttctgg tacgatctct
     9421 tgaataaata aaacccctaa aaagcagatg atcccaaaaa tgccgaaaac gatttcttga
     9481 gacatagaag cagtcatcac aggaaaaagc aagccaacta ggaaagaacc aatccaatta
     9541 aaagaagagg ctaggccaga tgctcgcccg cggacagcta gtgggaatat ttcgcctacg
     9601 attacccatg tcagtggagc ccatgtgcaa gaataaaatg cgacataaat acataaaaac
     9661 aagagaatca acaacgaatt tgtatgagca tccagtacag ttcctaaaac agctggtagg
     9721 atgaaagaaa gtcccatcac gctaccgcct attttcaata aagttcgccg attgaacttg
     9781 tctgcaataa ccaaaaagag caaagaacca gcaactaaaa tgattccttg gatgatcggc
     9841 cacatcaatg catcgctcgc tgcatgccca gtcgcttttt ctacgatcag cggaatatag
     9901 taaaaaatag cattagctcc ttgaaattgc tggaatactg ctacaccaat accggcaatg
     9961 actaaggaac gatatttttc taaaaacaaa gtatgccaag aagttccggt tgtcttttct
    10021 tgtttgacat tcagctggat ttgttcgaat tcctgttcag cttcttttgg tgtccgaata
    10081 tagttcaata ctctttttgc ctcttccaag cggccagatt gcataagaaa acgcggtgac
    10141 tctggtagag ctaaaacacc gaaaaataaa atcagcgcgg ggacagcagc gagccctaac
    10201 atcacgcgcc aagccatcgt ttcaggcaac ccttttagca aataatctgc aacataggaa
    10261 aggagcatgc cagaagcaat catcacttga ttgatccctg acaatcttcc ccttagtcta
    10321 gcaggagcca tttcagacat ataagcagga actagggcag atgcagcgcc aacagctaaa
    10381 cctagaagaa tccttgctgc aatcagaaaa taagaaccat tgtgaggggc agatgcagaa
    10441 aggacagaac cagccataaa aataagtgca gaaagaagaa tcatttttcg tcgtccaagc
    10501 ttatcagaaa gaagaccaga taaagagccg ccaaggatcg cacctaacat gaccgaagac
    10561 gtgatccatc ctatcaaaga agctttacct gccaaacccc aatcatgctg taaaaaaggt
    10621 aaggcgccgg tcataacacc aatatcatat ccaaatagaa tgccgccaaa tgcgccaaaa
    10681 aaataaataa acgatgagga aacttttttt tcttttttca taagtattct ctccttctag
    10741 aatttcgaga aggcggttga tcagattttg taagcaattt cattttataa cttatgtacg
    10801 tacaaaacaa gtgtttttta ttgtattctt gtaaataact gcttgacatc tacgtacaaa
    10861 agtatacaat aatcattgta agcgctacta aaaagacggt ttgatcaggt ttgacgagaa
    10921 ggagggagtt aaatcattat gaaggaagta aatgcgcaaa gtatgttaga tgtcatagat
    10981 ggtcgtacat ctttagggat tgagtttgga tcaactagga tcaaagctgt attgattgat
    11041 tcatctttta ctcctattgc cgctggtagt tatgagtggg aaaacaaact agaaaatggt
    11101 atctggacct attcattaga agatatttgg aaaggactgc gcgtcagtta ccgaaaaatg
    11161 gcagcagaag tattcgaaat ctatggcgag aatcttacga caatcgggtc gattgggttc
    11221 agtgcaatga tgcacggtta tctagcattt gatagcaagg aacagttact agtaccattt
    11281 cgtacttggc gaaatacaat cactgaagaa gcagaacgaa tattaacgga aaaatttcaa
    11341 tacaatattc cgcagagatg gagtatcgct catttgtatc aatctatttt gaataaagaa
    11401 gaacatgtga aagaaattaa ttttttgaca acgcttgcag gttatgtcca ttggcagctt
    11461 actggtgaga aagtactagg cgtaggagat gcatcaggca tgtttcctat cgatccttca
    11521 acgaaaacct accatcaaga aatgaaaaat atttttggtc agttaatcga aaaagcgcac
    11581 gtagcgattg acttggatca cattttgcct aaagttctag tagcaggaga agaagctggc
    11641 aagttaagcg aatcaggggc ttatctgatc gaccctacgg gaaatttgag accaggaatc
    11701 ccggtttgtc cgccagaagg ggatgcagga accggtatgg tcgctacgaa cagtgtagcc
    11761 aaagggacag ggaacatttc ggctggtact tccgccttcg ctatgatcgt attagaaaaa
    11821 gaactagaaa atgtccatcc ggaaatcgac atggtcacta caccttcagg agaactagta
    11881 ggcatggttc atacgaataa ctgctcttcg gatatcaatg cttgggtcag tatttttgaa
    11941 gaatttacca attctttggg tatggagata agcagagata aattattcac ggtattattc
    12001 aacaaagcgc tagaaggcga tacggattgc gggggattac tttcttatgg ttatttttcc
    12061 ggtgaaaaca ttacaggagt aaatgaagga aggccattat ttgtacgtac accaggtagc
    12121 cgttttgatt tagcgaattt catgcggatc catttagcca gtgcttttgg tgcaatgaga
    12181 atcggaatgg atatattgaa atctgagaat gtccaaattg atcgtttagt ggggcatggg
    12241 gggattttta aaacaccaga agttggacag cggattctag catctgctat ggaggcacca
    12301 gttacagtga tggacacagc cggagaaggt ggagcgtggg gaatcgcgtt attagcagcc
    12361 tatatgatgg atcgacatgg gaagtcttta gaaaacttct tggcagaaga cgtgtttgga
    12421 caagaccaag gaatcaccat cacaccttca gcagaagaaa tagcaggcta tcagatattt
    12481 atgagacgtt accgtgaggg gatcgacatt gaaaaaacag cgattcatca attgcaagaa
    12541 aaaggagtga tagaaaatgc tggaacaact gaaagaagaa gtataccaag caaatcttga
    12601 cttgcctaaa cacggactcg tcaaatatac atggggaaat gtgagcgctt ttgatcctga
    12661 aacacgatat ttcgttatca aacctagcgg agtcagttat gaagagatga cagcagacga
    12721 catggtcgtt gtggacttgg acaatcatat cattgaaggt aaactcaatc cctcttcgga
    12781 tacgcctact catgctgttt tatatcgttc atttcctcaa atcggaggaa tcgtccatac
    12841 tcactcgaca tgggcgacga tctgggcaca agcaggattg gatgttcctg cgatggggac
    12901 gacgcatgca gatacttttt atggatcggt accttgtgct cgatttttga cacaacagga
    12961 aatcgacagc gggtacgaat atgaaacagg aaaagttatc atagaaacat tcaaagaacg
    13021 caaaattgat cccttagcaa ttccaggggt acttttacac ggacacggtc catttacttg
    13081 ggggaaagat gcgcaaagtg cggtaatgaa cgcagtcgta ctagatgaag tatgcaagat
    13141 gaatttattt accagacagc tcaattcatt ttcagaagag ctgcctcaac ggattttaga
    13201 taaacattat ttacgaaaac acggaaaaaa tgcgtattac ggacaaaaca aataaaagga
    13261 gtggctaatt atgttgaata ttggagaaaa agaattttgg tttgttgtag gatcacagca
    13321 tttatatggg gaagaagcac tgcgagaagt aaagaaacat gcacaagaaa tggtcgatga
    13381 actgaatgaa aatggccaat tgccttatcc aatccgcctt caagaattag cagttactgc
    13441 tgatacgatc acaaaaatca tgaaagaggt caattatcga gaagaagtag caggtgtgat
    13501 cacttggatg catactttct caccagcaaa aatgtggatc cgcgggacaa agcttctaca
    13561 aaaaccattg cttcatttag ctacgcaata taacgaaagc attccttgga aaacgatcga
    13621 tatggatttc atgaatttaa atcaatccgc tcatggtgac cgcgaatatg ggttcatcaa
    13681 tgctcgatta aataaacaaa ataaaatcgt cgttggctac tggaaacgac ctgagatcca
    13741 aaaagagatt gctgattgga tggatgttgc agtagcctat aatgaaagtt ttggaataaa
    13801 agtggctcgt tttggtgaca acatgagaaa cgtgggtgtc acagaaggag acaaagtgga
    13861 agcacagatt caatttggtt ggactgttga ttattttggt atcggagatc ttgtacaagt
    13921 aatcgatcgc gtatcagacg aagaagtcga tcaattattt gaagagtaca aggaattgta
    13981 taccttcgac tatggtgatt atgaagaaaa gacatgggag gaacatgtaa aagtacaagc
    14041 tcagcaggaa atcggtattc gccgtttctt agaagaagga ggctataacg cttttacgac
    14101 taattttgaa gacctgtatg gaatgaaaca actacctgga ctagctgtac aacgtttgat
    14161 ggcagaagga tacggttttg ctggggaagg cgactggaaa actgcagcga ttgatcggtt
    14221 gctgaaaatc atggctcgag ggaaagatac aggattcatg gaagattata cttacgagct
    14281 agctagtggc caagaagcaa tcttggaatc ccatatgatg gaagtagatc cgacgcttgc
    14341 tgcgacgaaa ccaagaatcg ttgtttctcc tttatcaatg ggagatagag aagatccggc
    14401 gcgtcttgta tttgatggga aagcaggaga aggggtagtc gtatcgatgg cagatttcgg
    14461 tacgcattat aaactgctga tcaatgaagt agaagccttt gaaccaacga ctgaagcacc
    14521 taatttacct gttgcacgtg tgttatggaa aactaaaccg aatttccatg aaggtgtcca
    14581 ttcatggatt caggcaggtg gcggtcatca taccgttgtc tcgctaaatc taacaacaga
    14641 tcaaattgaa acgtgggcaa aattagttga gttggaaaca gtggttatta gataaaaaga
    14701 ggttgtgaaa aagccactta gttttaagaa ataagaagag attgacgaaa tttgcttttc
    14761 aacttttttg aaatttagtc ttattatcaa tgtcagctaa cggaacacca tttatcaaga
    14821 tatctcaccc tcaatatttt gctctcgaaa ataaggctta tggcagtaac ttgtcatagc
    14881 cttattttta tcactcagcc tactctgctc taactgctct ttttgcttct aggaagtcaa
    14941 atggtaaaca gttgtctaat tgcagaaatt tcgagatact tgttagtaag gagggggttt
    15001 tatgtatcac gttcatttga ttgatgctat tccagaacaa gagatagaaa agttacaagg
    15061 ggaatatttc aaaataatag atgatttgcc agccgaagga atagttattc gtagtacgaa
    15121 agtgaaagat gaatggctta caccagattt gctagcaatt tcaagagctg gtgttggggt
    15181 gaacacgatc aacgttgaaa aagcttctga aaacgggacg atcgtcatga atacaccagg
    15241 tgtaaatgct aatgcagtaa aagagcttgt tttgtgctgt ttgctcttat cttcccgtcc
    15301 gataatcgaa gcaagtcgta tggtacaaac actaacaggt cctaatattt tagaacaggc
    15361 agaaaacaaa agaagtgctt atgtgggacg agaattgcaa ggcaaaacca tcggtcttct
    15421 tggtcttgga gcgataggca caaaagtagc gctaagttgc tatagcttag ggatggatgt
    15481 cttgggttat tctatccgcg atgcccagtt ggattatgtt cggcaagcag atttagaaac
    15541 agttctttct acctcggatt acatcgtagt catgctgccg ttgacagaag atacaaaagg
    15601 attgatcgat caggcgaata tagaaaaaat gaaaaaagat gctgtactta tcaatgtagg
    15661 aagaagtgaa attgttgata aatatgctgt tatgcaagca ttggaaaaac aacatctggc
    15721 taaatacttg actgattttc cagaagaaga gttcttagaa aatgaccgta tacttatgtt
    15781 gcctcatcta gggggatcga ctcaggaagc tttggcagac agcggacgtt tggcagttga
    15841 agccttaaaa gattatcttt tgttcggtac ggttagagaa gcggtcaatt atccatccgc
    15901 acgaatgttt ttccaagcgc cgtttcgatt cactatattt tatcaaaaga aaaccaatat
    15961 attagcagag atattttctc tgctgaatga gaacgagcta gcgatcgcag atatatcgcg
    16021 taaccataag aatgagcatg tctacatatt gattgacata gacagtactg attttgaaca
    16081 gctcaatcaa gtgaaacaac agctagaaaa aatttctgga gtacgaaagg tccgcttatt
    16141 aaagaaacca gaaaggccat aaagaaagag aatatttgga tttcattaat tcttctttcc
    16201 gttctttact ttcgaatttc gtacaactaa aataagagaa gcaacattct tttattgata
    16261 gaaatgaagg agcaagcatt tgaaaagata tataagcaga ctttcgacca aacagaaaaa
    16321 gattttaaaa ggaacagcag caatcatcag tatctttttt cttgttgttt ttagtaattt
    16381 atttttgcag tggtgtcaaa atgatctatc cgtagacctt gcgttgaaat ttgctttttc
    16441 ttggcatacg gaaaaattct tcttagcctg tctcgtcttg ctgattattc ttatttttct
    16501 gattgcacta gcaggatctg tccctttagg ttcccttacg tatgtcgtag ccatcggtgt
    16561 attaggtttt gcaaattata tgaaaatgag ttatcggcaa gaaccgattt atccagacga
    16621 tttgaagatg atcactgaaa tcggattgct gaaagatatg accggcacta tgctgtttac
    16681 agtgatctta gctgccgcag gaaccgtgat aggattgttt tgctggtaca tgtttcgaag
    16741 cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg acactgcttg tggcaattgg
    16801 actgctaggg tatatcagta atttcaacaa tcccgataat ttgttgcgaa aagcatataa
    16861 taaaacggca ctatggatac cttacagcca gaaaatgaac tactataaca ctggttttat
    16921 cggtgggttt ttgtacaatc tgaaggtaga accaatggat gaacctgaag gatattcgaa
    16981 agaaaagatc aaagaaatca ctgaaaaata ccagaagcta gccgatgaaa aaaataaaac
    17041 tgtagaggaa gagtcgccga acatcgtgtt cgtgatgagt gaaagtttct cagatcctag
    17101 tcgattaaac ggcgtagaag tatctggtga gccattggca gattattatg aagtagctga
    17161 tcaaacatat agtggcaata tgctttctca aaactatggc ggtggaacag caaatatcga
    17221 atttgaagca ttgactggtt tttctatggc gttatttaat gcgcaactca ccacccctta
    17281 cacgatgctt gtaccaaaaa tggatcagct tccttctatc gtttcagcgt taaacgcaca
    17341 atcataccag acgacagcga tccatccata caatacttcg atgtataaaa gagaggatgt
    17401 ttatcagaca ttaggatttg accagttcat cagcgagcgt acgatgacct atacggatac
    17461 gatagagaat aatccatata tttctgatga gtcagcttac aaagaagtaa tgacacttct
    17521 caaggaagaa aaaacacctc agttcattca tttagtcacg atgcagacac atatgcccta
    17581 cgatggaaag tatgataatc tagactatcc tgcaaaaact tcagatggaa gtgggacact
    17641 tgacttagag aattatctgc aagatatttc ctatagcagc acggcactga agcagttcac
    17701 ggaagaattg aagaatcttt ctagaaggac tttagttgtt ttttgggggg atcatcttcc
    17761 agggatctat tcagatacga tccaagcaaa aaatgacaaa caaacattgc atgaaacaca
    17821 atttcttatg ttcgacagta aaggagaatt agaaaagcaa acgactcacg atgcaatcac
    17881 cagtccgttt tattttgcag cgaatctaat ggaacagacg aatcagacaa caaatggctt
    17941 ttatcagctg cttttatcat tagaacagga acttccggcg tttgaacgag aactgtacta
    18001 tcaaaacgga cagtggtata aagaagcgca attcaatcgc tcgcaacaag agatttacga
    18061 cgaatatcaa ttgatccaat atgacattgt cgcagggaaa caatatagtt tagcagatgg
    18121 tttctttgag cacgaataag aatggcgagc tgtatagaaa ccattaaatt ttttcaagta
    18181 cttgtcattt gaggataaaa acaagtagaa taaataataa caacaatgga gaaagtagga
    18241 aaacgaacat gttaccgaat attggctatt atgtgcagtc catcgatcaa atcgtcaagg
    18301 aaacagaagc aatcggagaa ggaatgcatc cgaaatatga agtgatcaga aacgcaattg
    18361 atgaaggaaa aacaagtgaa ctggacaaat cgactcttga agagatcatc ctgttatttg
    18421 aagaaggaac aaaaaaatat cgggcaatgc ttatgaaagt cagtactttg cgtccaccag
    18481 caaaagcaat gggcattcat aaaaattttg aaaaagctta tctttcatat gtagcaggct
    18541 gtgaagaaat gatccaaagt atcgatcttg aatcaggggt agatgaagat ctattcaata
    18601 catctgaagc aaaacaagac caagcgacag accaattgac agctgccatc caaaaaatga
    18661 ctaatttgtt gttgaaaaaa taaaagaaat ctttgataac aataaaaact agagttatct
    18721 aatataaatg taatattacc tttatgatag agtgatcaaa atcctttatg ataatgacat
    18781 acctataacc aacaacaacc ttgtacttac tcaaaatcga gtaaatactc cttttccgtc
    18841 gagtctaacc atctcggcgg ttttttattt gtagaaaaga acatcaggat agacggaaaa
    18901 gtcttgtcct gatgttcttt tcagatagta tggaaagctg attagttttc tacattctgt
    18961 acatattgtt caaagtagtt ctgtaaatcg gctttgagtt tctgatattc ctcttcgcta
    19021 tactctttat cttcgatttt cccatcaaag aaaggaatat ttagttcttc ccgtagtctg
    19081 tctaatacgt cttgctgatt catttcattc tccttttctt actaacgatc aaaataaaca
    19141 cagacacatt ctggtacata gacatctttt ttcagtaatt ccagtaatcc tgtttcagga
    19201 tgacggcgat ataatgtcag attatcggtg ttctgattgg cacacacgac aaaattattg
    19261 cttggatcta gtgcgaaatc gcggggaaaa tccccttctg ttgagatgat ttgcttgttt
    19321 tgaatgtggg aaccatcttc aaaaactgcg aatacagcaa tcgagttatg tcctcggtta
    19381 gaagcataaa ggaacttgcc atcatttgaa atgcggatag ctgcccctcc attttcaccg
    19441 tcgaattctt ctggcaaggt cgatattttc tgtaattctt caaaagcacc tgtttcttta
    19501 ttataagaaa gcacgcggac tgtgctgtct aattcaccaa ataaataagc gaattgtcca
    19561 tttggatgaa agacaagatg gcgagggcct gttcctggct ctgcagtgaa ggtagttaca
    19621 agattcaatt tgccgttttc ggacacatca taagtataca cacgatctgt accaagatca
    19681 catacgacta atcgttggtc aggagtcaga tccgtatagt ggacatgcgc atgatcttgg
    19741 tttttatgag gacctgtcgc ttcttgatgg aatattgaat ctgctgcttt cagttcgcca
    19801 tctcctaata tatggtagac atttacttca cctttatgat agttcgcccc gtatagtaat
    19861 tgacgtgctt catcaaccgc tacataacac aaaggagcgc cagactctgt taccgcattc
    19921 aaaaaatgaa aatccttatc gtacgcaccg gcaccaccta atccatccac actagtaact
    19981 gtgtaaagat tgccagcttt actttttgct acataggtag ggctattttc ttcaacagct
    20041 aatgaaaggt tggctaattc ttctttttct gtatcaaggt cgatcgtata gattccttta
    20101 ctttcgcgac gcgtataggt tcctaaaatt atcttttcga tcatatgagc tgattcctcc
    20161 taattgtaat gaaatagttt tcgtttttat tctatcacat aattattcca cacggctaaa
    20221 gaatgagaat gtgttatact gtttttgaaa gcaattaaag gagagagtgc aatgattcag
    20281 gcaaagatta cagaaattgg gacagaagca ataaacaagg aagaacccat gctgattttg
    20341 ttcgataaaa cagcaacagc tgccttacga aaatattcgg tgattcaaga agtatcaaaa
    20401 gacgaacctt tttctttgaa agcaggcgga acaattgctt ttgatgatca ggtatatacg
    20461 atcgattatg tcggtccgat ggccaatggg aatttaaaga cagtagctca cgtatcattg
    20521 atttttgatg cttgtccaga agaaggacaa attgcaaatg gaatctatct tcatccgcat
    20581 agactgcctg ctattggttt gggaagccaa attttttata actaacagga ggaattatgg
    20641 aaggaaaaaa taagaagaac agaccggtat cgtggttttg gcgctggttt ttaaataatc
    20701 aagtagtcac agccttactt gttgtattgt taatattgct gatcgtcttt ttatttacaa
    20761 aagtttcata tttgtttgca ccaatctggc aatttttagc aatcgtaggt ctgcctatca
    20821 ttttagcagg aatcttgtat tacttgatga atccggtagt tgattacttt gaaaagcgaa
    20881 aagtaccaag gctttacagt atcattgggc tgtttgtttt ggttgttgca ctaatcgcat
    20941 gggggagcgt agtgatcatc cctaagattc aggaacaaac agtgagcttc atcagtaact
    21001 tccctcaata tgtagatacg attgataata aattaaccga gattttgcgg gacccgctgt
    21061 tcaaccaatt tcgcgaacag ttggaaacag caggtgataa gttcatgaat tcggcaggcg
    21121 atatgatcca agatatatct aagagtacag ttcagagtct tggaagtttt gtcggcgcgg
    21181 tagcgactat tttagtggct ctgctgacga tgccgtttat cttattttat ctattgaaag
    21241 acggtaaaca attagcacct tattttgtca aatttttacc gacacgcatg caaaaaccga
    21301 cgttgaatgt gctgagtgaa atgaatagtc aagtttcttc ttatataaga ggacaactaa
    21361 ctgtggcttt tgctgtggca atcatgttta tgattggttt tgcggtcatt ggtttagagt
    21421 atgctgtcac gttggggatt attgcgggtt ttttaaacct gatcccttac ttaggatcat
    21481 ttttagcgat gattccggct atctttttag ccattgtagc aggtccattc atgatcatca
    21541 aagtactgat cgtttttgca gtagagcaga caatagaagg acgcttgatc tctccattag
    21601 tactagggaa tcagttagca atccatccag taacgatttt attagttctg ctgacttctg
    21661 ggaaattgtt cggaatcatc ggtgtgatct tggggattcc tgtttatgca gcagctaaag
    21721 tagtgattac tcatgttttt gaatggtaca caacaatctc ttctctttat gaagaaaatc
    21781 cgttgaaaaa gcagaaagaa taacaaaaag agacagatgg gaaaatatcc aaggcacaaa
    21841 tgctgctttg aagatttttt cctctgtctc ttttgtatgc atttatctca atttttaaaa
    21901 tgatttacct aaagcagcag ctttatctaa tgcattttgc atcaactcat ctgcacggtc
    21961 agggtcataa tcgattcctt cgatgaatag ctgatctact tgatcgatgc ctacaaagtt
    22021 taaaatacct tttacatatt gagaggcaaa gtcttgtccg ttgtatacac cgccattaga
    22081 ttggatgtgc agtgcttttt tacctgtcac taatccttct ggcccgtttt ctgtatagcg
    22141 gaacgttttt ccagctacat tgacggtatc gaaccaagct tttaattttg ttgggatatt
    22201 tagattccat aaggcgtttg cgataacgat tttatcagca gcaaggaact gatcagtcaa
    22261 ttcattgaaa cgagcaattt tttgttgttg actttctgat aaggaagtga attctgcacc
    22321 agcacgtaat gcttcccaac ctgataataa ttcttcatcg atttccggaa tatcatcgcg
    22381 ataaagttcc aaaacagtca cttcatcatc tggatgattt tctttataac ttgttaaaaa
    22441 agacgtcaat gccttaactg tgcgcgattc ttctgcagtt aggggatgac ctttgactac
    22501 taaaacagat gccatatgct taccttctcc ttttattgct atgattgtta taactatgac
    22561 taacaaaatc agtttactcg aaaaagaaaa ggctatcaag tcatattgat aaggttgaag
    22621 aataaataac taaaaaaaga cagggtataa aagaggctgt gacacgattt tttgtcacag
    22681 cctcttttga atctaacaag caatattcaa gcaacactcg actgaagagc ttccatccca
    22741 ccaatgatat aaacagcacg aaaaccatta gcagtaagtt gatcggctaa tgcttctgat
    22801 cgcctgcctg tatagcttag aatataataa agtttctttg gatctagatt ttttaattgt
    22861 cttggcaaga tagcagcggg gatagaaagc gagtggctta agtggttttt tgtgtaatct
    22921 aaggtatttc ttgtatctaa aacgatgctg tcagcatcga gaggcaattt attgaagtct
    22981 tggacactaa ttgattgggc catttatctc atcctttgtt tagtatgtgg ctattttaat
    23041 gggcaactct cattaattca aatggaagga ttcctcttat cactttttta atgtatcgga
    23101 tgataatgaa aaaaactatg caaatgtctt gcaaaacagg taggaggtgt tataatgaca
    23161 tagcgtcata tgacaatgtg tcatcttgaa agaaaggaga atccatatgc cgacacagac
    23221 atttttccat ttgcctaaag aaaaacaaaa aagactaatc gaggctgcga gaatcgagtt
    23281 ttctagagta cccttaaaag aagcttcgat tgctaatatc gtcaaattag cggatatccc
    23341 tagaggcagt ttctatcagt attttgaaga taaagaggat ctttattttt attactttga
    23401 gacaatgaga cgtgacagct ctaaagatat gttacaacta atgaaagaat ccgatggtga
    23461 tctattcaaa ggattcgaat cctatttttc taaaatgatt tatgagattt tggaaggcga
    23521 gaatgcttct ttttatcgta acttgtttat gaacatggat taccgtgcct caagaaaagt
    23581 cgctcctcat acgagtcgcc aaaaaacgcc tcacgatcca cataaagaac acaaacaaga
    23641 tgcaaaagag ttgcttgaat tgatcgatcg gacgaagctg aaagtaaaaa atgatcatga
    23701 attggaatta ctgatccaat tagtgatgaa tactgtattt acaacaattg cgcattcgta
    23761 tcgcttattt tcaacatctg aagattactc agtagagcaa gcaatcagtg aattcaattt
    23821 gaaactttct tggctgaaaa acggagtata taaatagaaa gaagggaact agatgctaaa
    23881 aatcgtaaaa cggatctcct tgacttcagc aattgcagct gcagttttca tggtgatcca
    23941 agtactggca gatttatatt tgccgacact tacctccaat attattgata aaggggtagc
    24001 gcaaggagat gtcgattata tttggcatac aggttttgtg atgatcggct tttctttgat
    24061 cagtatctta gctgctatcg caaatacatt ttttgctact cgtgaatcac agaaactggg
    24121 taagcaattg cgtacggatg tctataaaaa atcagagagt ctgacaaaag atgcgtttga
    24181 taaatatgga acggcctctt tgatcacaag aacgaccaat gatgtgactc aaatccaaat
    24241 ggttacacaa atgtttttac gtatgatgat caatgcacca atcacgttga ttggcgcgag
    24301 catacttgct tatcaaaaag atcatcagtt gacaaaaatc ttcttagttg tcatcccagt
    24361 aatgattatt ttgatcggcg gaatcatgta ctttgctgtt ccattattca aaagtatgca
    24421 aaagaaaact gaccgtttaa acctggtatt tcgagaagga ttgacagggg tcagagtgat
    24481 tcgagcattc gataagacac gctttgaaga aaatcgtttt gatttagcga ataaagatta
    24541 tacaaatacg gcaatcaaag ttaatacgat cgtcgcattg atgatgccaa tgatgacatt
    24601 gatcatgagc ggtacaaacg tagcaatcac ttggtttggt ggacactaca tagcagatat
    24661 gactttggaa gtcggtaatc tgattgcatt tatgacttat gcgatgcaga tcttgatcag
    24721 ctttatgatg ctttccgcta tttttatcat ggtgcctcgt gcacaagctt cagctgaccg
    24781 tattaatgaa gtgttggatg aaaaaatcgg tatccatgac cctgaaaatc ctaaaacagt
    24841 ttcttttgct ggaaaaaacg caacattggc cttcaatcat gtcaattatc gatatcatgg
    24901 agcagaaaaa cttgcattgg aagatattga tttccaagcc aagtcgggcg agatcgtcgc
    24961 aatcataggg gggactggtt caggtaaaac gaccttagtc aatttgatcc ctcgattata
    25021 tgacatcgaa tctggttcga tccaaataaa tggaacggac attagtgaca tgactcaata
    25081 taatttgcga gaattaatgg gctttgttcc tcaaaaagca gttctgttct ctggaacgat
    25141 ccgagacaat atgcagtatg gtaagccgga tgcaacagat gaaatgattt ggaaagcatt
    25201 agaaatcgca caagctaaag attttgtttc agagatggaa gatggcttag atagccatgt
    25261 ggaacaagga ggaggaaact tctctggtgg acaaagacag cgtctggcaa ttgcacgggc
    25321 attagtcaaa acagcagata tctatgtgtt tgatgattcc ttctcagcgt tggatttcaa
    25381 aacggatgcc aatcttcgcc aagcattgaa aacgaatatg aaagaaagta tcaccgtttt
    25441 agttgctcaa cgagtaagta cagtaatgga tgcagatatg atccttgtgc tagatgaagg
    25501 aaaactggtt ggcaaaggaa cacatgaaga actattagca acaaacgaaa cttatcaaga
    25561 aatcgttcat tcacaactaa gagaggagga cttagcatga gtaaaacaaa cacacgtccc
    25621 tctcatggac cagcaggcgg tccaggacat ggtggacctg gcagaaatct cggtgtaaaa
    25681 ggtgaaaagc ctaaaaattt ctggggaacg gtacgccgat tgtttggcta tatgtctaaa
    25741 cgtatggttg cgatcatcgc tgtatttgta ctagcaatag cagccgtgat cttccaaatc
    25801 caaacaccaa aggtcttggg gaaagcaacc acagaaatat tcaaaggtgt aatggctggc
    25861 gctcaacaga tgcaacaagg ccaacaagta acaaaatttc cgatagactt tgataaaatc
    25921 gcgcagatca ttgcgacagt aatcgtgatg tatttgattt ctgctgtatt caacttttta
    25981 cagcaattta ttatgacacg tgtttcccaa cgaacagttt atgaattacg tcatgatttg
    26041 gaagccaaaa tgaatcgtgt accgatttcc tattatgata cacatacaaa tggggatatc
    26101 atgtctcgag caatcaatga tatggataac atcgcaagca cgctgcagca aaatttgact
    26161 caatttgtaa ctagtgcagt cacattgatt ggggtcatcg tgatgatgct ttcaatcagc
    26221 tggcaattga cgttagtcgc gttattgatg atccctttaa gcttgatcat cgtgatgatc
    26281 gtagcgccga aatcacaagt gtttttcgca gatcagcaaa aaagtttagg tttattgaat
    26341 aaccaagtag aagaaacata tggtggtcat accattgtta aaacattcaa tcatactgaa
    26401 aaagaccagg aagtatttga aaaagaaaat cagaatctgt atgccgctgg ttggaaagct
    26461 caatttattt cggcaatcat catgccgttg atgaatttcg ttaaaaatct aggctatgtg
    26521 ttcgtcgctg tactaggcgg agtaaaagtt gcaaatggga acatgacatt aggggatgtt
    26581 caagcgttct tgcaatatac aacacagttc tctcaaccaa tcactcaact tgcaagtttg
    26641 atgaacacta tccaatcaac agttgcttca gccgaacggg tatttgaagt gttggatgaa
    26701 gaagagatgt ccaatgaaaa atctggcctt ccagaagaaa aagatacacc atacaaagtt
    26761 cgttttgaaa atgttcaatt cggctacaca ccagataact tgctaatgac tgattttaac
    26821 ttggatgtca aacctggtga gatggtcgct attgtaggtc cgactggtgc agggaagacg
    26881 acactgatca accttcttga acggttctac gatgtttctg gaggtagtat tcgttacgat
    26941 ggtgtagata cccgtgatct ttcacgtgat gaattgcgcg cacagttttc aatggtactg
    27001 caagatactt ggctgtttac aggaagtatc tatgacaata tcaaatatgg gaacgacgat
    27061 gcaacagacg aacaaatcat tgaggctgct aaagctgctc acgtagacga ttttgttcgt
    27121 aaattgccag aaggatataa cactgtatta aacgaggatg caagcaatat ttcgcaaggg
    27181 cagcgtcagc taatcacaat tgcgcgggca ttcttagcaa atccagatgt cctgatcctt
    27241 gatgaagcaa catctagtgt agatacacga acagaaatac tgattcaaaa agcgatgaac
    27301 cgcctattgg aaaaccggac aagtttcgtt gttgctcacc gcttgtctac aatacgcgat
    27361 gcagacaaca ttattgtgat gaatcatggt tcgatcgtag aaacaggaaa ccacgataca
    27421 ttgatggaaa aagacggatt ctatgcagat ctttataaca gtcagttttc tgaagaagaa
    27481 gcttcataga atagtacaaa tttattattt tttttagggg ctgtgacaaa agtcgtgtca
    27541 cagtcccttt ttccgaataa acggtgttca atcagctgtc cttcggtatt agctcaaaaa
    27601 acggcaaaat atgagaagct atttttccgt tttgtatctc taatactcag gtcaaaccgc
    27661 tgattttaca acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
    27721 aatagtcaac aaacctgaga agctgttttt tgaaattgct ccttgtgact cggagttaaa
    27781 cggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
    27841 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
    27901 agcgactttt tcacaacctc tggtacacgg tgttccaaaa gtagcttctt cggaaataag
    27961 ttggaatttc acaaaaattc aagaagtaat tttcgtaaat tccttcttat ttcttgaagc
    28021 tgattacttc tgtcacaacc tctttttatt attgagaatt tggtcgagga ataatcgttt
    28081 ttgccatcca aatatccggt ttgtcccagc catttgcatt tggtaagaca cctacgattt
    28141 tataacctaa tttttcatag aattcatacg gatgttcacg aaggttctgg atggaagcca
    28201 ctttatcaaa tgtatgctca tacaggtccg tttgacttaa cgttgttcca tggtctaaat
    28261 catccgtacc taaataaatc gtgattcctc ctctggaagc tacttctttt tctaagtaat
    28321 tgactaatcg agtacctatt tggttctttc gtcgggagct ttctacaact aatggatgca
    28381 attcccaacc tgtgataccg tattgaggga ttgcaccaat aaatcctact aactcatctt
    28441 ggtctaccgc tgctaccgcg attcgttctg gattcatcat ttcttctact tcttctgccg
    28501 agctgtctcc atattcttcc ggccaagtca gtctcagtaa atcagaaagc tgatctttca
    28561 atactggatt attacggtca aattcactga ttatcatacg tcttccccct tgttcttgct
    28621 tattctaaca gtccttttga aaaaacgaaa tgttctcgct caaaaatctt tagctaccaa
    28681 tcagtatctc tattctttca ttatttatct tacttataaa ataaaaaatt atacagaaac
    28741 actttattca aaataggaaa gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg
    28801 ttcatcatga tatagattat cttttgcttt aaaaaaaacc aattatgaat tgacaaataa
    28861 aacctgtttt catataataa tcttaatttt tagaattttt agataattac
//
LOCUS       DAYYCM010000033        27815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000033 DAYYCM010000000
VERSION     DAYYCM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.034"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(547..909)
                     /locus_tag="ACHAY1_001487"
     CDS             complement(547..909)
                     /locus_tag="ACHAY1_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593489.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="ACHAY1_001488"
     CDS             complement(983..1363)
                     /locus_tag="ACHAY1_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8593490.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="ACHAY1_001489"
     CDS             1445..2620
                     /locus_tag="ACHAY1_001489"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8593491.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2697..3281)
                     /locus_tag="ACHAY1_001490"
     CDS             complement(2697..3281)
                     /locus_tag="ACHAY1_001490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8593492.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3482..4480
                     /locus_tag="ACHAY1_001491"
     CDS             3482..4480
                     /locus_tag="ACHAY1_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8593493.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4592..5101
                     /locus_tag="ACHAY1_001492"
     CDS             4592..5101
                     /locus_tag="ACHAY1_001492"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8593494.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5131..5790)
                     /locus_tag="ACHAY1_001493"
     CDS             complement(5131..5790)
                     /locus_tag="ACHAY1_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8593495.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5792..6556)
                     /locus_tag="ACHAY1_001494"
     CDS             complement(5792..6556)
                     /locus_tag="ACHAY1_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8593496.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6564..7241)
                     /locus_tag="ACHAY1_001495"
     CDS             complement(6564..7241)
                     /locus_tag="ACHAY1_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8593497.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7278..8663)
                     /locus_tag="ACHAY1_001496"
     CDS             complement(7278..8663)
                     /locus_tag="ACHAY1_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8593498.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMNGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8873..8957)
                     /gene="ffs"
                     /locus_tag="ACHAY1_001497"
     ncRNA           complement(8873..8957)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAY1_001497"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="ACHAY1_001498"
     CDS             complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="ACHAY1_001498"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8593499.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9601..10458
                     /locus_tag="ACHAY1_001499"
     CDS             9601..10458
                     /locus_tag="ACHAY1_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593500.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10539..10874)
                     /locus_tag="ACHAY1_001500"
     CDS             complement(10539..10874)
                     /locus_tag="ACHAY1_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593501.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10941..12311)
                     /locus_tag="ACHAY1_001501"
     CDS             complement(10941..12311)
                     /locus_tag="ACHAY1_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8593502.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12389..12925)
                     /locus_tag="ACHAY1_001502"
     CDS             complement(12389..12925)
                     /locus_tag="ACHAY1_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593503.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13234..14715
                     /locus_tag="ACHAY1_001503"
     CDS             13234..14715
                     /locus_tag="ACHAY1_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593504.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14818..15525)
                     /locus_tag="ACHAY1_001504"
     CDS             complement(14818..15525)
                     /locus_tag="ACHAY1_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8593505.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15604..15942)
                     /locus_tag="ACHAY1_001505"
     CDS             complement(15604..15942)
                     /locus_tag="ACHAY1_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8593506.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(16053..17015)
                     /locus_tag="ACHAY1_001506"
     CDS             complement(16053..17015)
                     /locus_tag="ACHAY1_001506"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8593507.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(17030..18361)
                     /locus_tag="ACHAY1_001507"
     CDS             complement(17030..18361)
                     /locus_tag="ACHAY1_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8593508.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18369..19049)
                     /locus_tag="ACHAY1_001508"
     CDS             complement(18369..19049)
                     /locus_tag="ACHAY1_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8593509.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19268..19747)
                     /locus_tag="ACHAY1_001509"
     CDS             complement(19268..19747)
                     /locus_tag="ACHAY1_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8593510.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19874..21553)
                     /locus_tag="ACHAY1_001510"
     CDS             complement(19874..21553)
                     /locus_tag="ACHAY1_001510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8593511.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22101..23603
                     /locus_tag="ACHAY1_001511"
     CDS             22101..23603
                     /locus_tag="ACHAY1_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593512.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23616..24278
                     /locus_tag="ACHAY1_001512"
     CDS             23616..24278
                     /locus_tag="ACHAY1_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593513.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24379..24582)
                     /locus_tag="ACHAY1_001513"
     CDS             complement(24379..24582)
                     /locus_tag="ACHAY1_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8593514.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24693..25877)
                     /gene="iadA"
                     /locus_tag="ACHAY1_001514"
     CDS             complement(24693..25877)
                     /gene="iadA"
                     /locus_tag="ACHAY1_001514"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8593515.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(26034..27773)
                     /gene="yfcC"
                     /locus_tag="ACHAY1_001515"
     CDS             complement(26034..27773)
                     /gene="yfcC"
                     /locus_tag="ACHAY1_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8593516.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8260 a         5802 c         4707 g         9046 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2641 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2701 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2761 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2821 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2881 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2941 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     3001 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     3061 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     3121 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3181 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3241 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3301 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3361 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3421 aacatttttg agaagcattc tcaattaaat tctgatatag tgtgaaaagg gaggaatttt
     3481 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3541 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3601 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3661 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3721 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3781 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3841 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3901 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3961 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     4021 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     4081 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     4141 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4201 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4261 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4321 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4381 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4441 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4501 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4561 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4621 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4681 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4741 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4801 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4861 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4921 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4981 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     5041 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     5101 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     5161 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5221 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5281 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5341 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5401 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5461 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5521 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5581 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5641 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5701 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5761 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5821 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5881 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5941 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     6001 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     6061 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     6121 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6181 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6241 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6301 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6361 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6421 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6481 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6541 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6601 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6661 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6721 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6781 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6841 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6901 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6961 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     7021 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     7081 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     7141 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7201 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7261 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7321 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7381 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7441 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7501 atctgcccaa aaatcttacc gcgaaatccc attccgttca taggatagtt acgtaaaaag
     7561 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7621 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7681 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7741 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7801 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7861 caactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7921 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7981 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     8041 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     8101 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     8161 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8221 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8281 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8341 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8401 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8461 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8521 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8581 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8641 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8701 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8761 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8821 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8881 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8941 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     9001 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     9061 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     9121 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9181 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9241 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9301 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9361 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9421 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9481 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9541 gataaaaagg ctgtaactaa ctttttttct atggtaaaat aaatacaaga ggtgaacaag
     9601 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9661 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9721 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9781 aaaaaacacc catggtatca ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9841 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9901 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9961 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt
    10021 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
    10081 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
    10141 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
    10201 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
    10261 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
    10321 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
    10381 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
    10441 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10501 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10561 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10621 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10681 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10741 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10801 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10861 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10921 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10981 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    11041 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    11101 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    11161 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    11221 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    11281 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    11341 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    11401 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    11461 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11521 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11581 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11641 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11701 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11761 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11821 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11881 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11941 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    12001 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    12061 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    12121 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    12181 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    12241 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    12301 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    12361 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    12421 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12481 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12541 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12601 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12661 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12721 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12781 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12841 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12901 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12961 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    13021 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    13081 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    13141 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    13201 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    13261 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    13321 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    13381 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    13441 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13501 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13561 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13621 aaacagctca atcattcctt aataaaattc catttacgga tcctatctgt aaaaaattgc
    13681 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13741 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13801 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13861 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13921 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13981 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    14041 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    14101 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa
    14161 cagtttcctc tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg
    14221 gcagttgttt ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt
    14281 ccagaagcca cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa
    14341 atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa
    14401 ttgatccaac acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata
    14461 accaaacagt ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa
    14521 gatgctgtta aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat
    14581 gagaaaattt cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta
    14641 tttggcaagt taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa
    14701 acaaagagga tgtgacaaaa ttcttgtcac atcctcttat tccaaataaa tggtattcaa
    14761 tcagctaact ctcggtatta gctcagatca aagcgctgat ttcacaactt ctttatttta
    14821 atctcttcgg ttgccgccga acaataagat caagcgcaac agctgcagga atgtcgaaag
    14881 cgctgctgca acataagtca gtgctgctgc aaataatacg tgtcttgcca tcggtacttc
    14941 ttcttccgtc agtagtccgc cttcactcaa aatttggatt gctcgccgag acgcgttaaa
    15001 ttcgactggt aatgtcacca attggaaaag taatgctaaa gaaaaagcga aaatcccgat
    15061 attgattaac gtagagttcc agctaagcaa tacacctacc ataatcaagg ggaacgaaat
    15121 catcgaacca aagttagctg ctggaacaag tgctgcacga atcttcaatg ggacataacc
    15181 cgaagcatct tgtaccgcat gtccgcattc atgagctgct acacctatcg cagcaactga
    15241 agtagactgt gcagttgctt gagacaaact caacatctta ttacctgaat tgtagttatc
    15301 tgtgagatct ccggcaattt gttgaacacc gacattattt atctgttgac tttgtaatat
    15361 atattgtgct gcttgtgtac cagtcacatt gtttttgctt ctcacttgat cgtattttcg
    15421 gaaagtacta ttgacgtacg ctgacgcaat tccggaaata actaatccaa gaatgaccaa
    15481 aagaaatgtc ggatctaaaa aaccatacat tccataacca tacattttcg tttcactcct
    15541 tttcgtttac tttattttat cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat
    15601 tctttaattc tttttcacaa attccgattt cagtttcatc ggtccaaatc cttctacctt
    15661 gcaatcgata ttatgatcgc cttctactaa acggatgttc ttgacttttg ttccttgttt
    15721 aatggcacct gacgcacctt ttactttcaa gtctttaatc actgtcacgc tatcgccctc
    15781 taacagacgg tttccatttg catctaatac cactaatccg tcttctatga cagtttcttg
    15841 ttcttctttg ttccattcgt tcccgcattc tgggcaaatc aacaagcctc gatcttcata
    15901 tgtgtactct gagccacatt ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa
    15961 tctttagtga gcactattgt aacggaaaaa gaagatcttg aaaagataca tttgtgattt
    16021 ttactacaaa aggcacgttt tcgaacgaag tatcattctg tttttctgct cttatacgat
    16081 tggcatatat cttggatctc tttatagatt tcctctactt ctgctaaatc ttttgcattc
    16141 gcaccacttg ctaatggatg gcctcctcca tgatggcgtt tagccaattc attgatgatc
    16201 ggccccttag aacgtaaacg tacgcggtaa taccctgaag gctgctgaac aaaaatcccc
    16261 caagccagca cttcgtcaat cactccagga agtgaaacga tcgaagctgt ctctgaatca
    16321 tcgattccat atttatcaag aatttcttgc ggaaggatta ttcttcctgc accattttca
    16381 tccacctgga tattttgata aagataaccg gaaagtttag cgactttcat cggcatttgt
    16441 tcaatctctc tgttcaaagc acttgcatca aatggataat ttctcaatat cgaagcaatc
    16501 tctaatgtac gggacgtcgt ggagggatac aaaaagcgcc ctgtatctcc gacgattcct
    16561 gcataaagta accgtgcagc cttaacgttc atctttaaag attcaggaaa catcaaagca
    16621 aattctgcaa tcatctcact acagctactt gcttttgtat ttacccacac aagatctcca
    16681 taagggtcat cattgggatg atggtcgatt ttgatcagtt tttctgccaa ctgatatctt
    16741 tgatcactga ttctgggtga attagctgta tcagtcacga tgactagcgc tccttggtag
    16801 aagacatctt caacttcttg catttctgct aaaaagcgaa gcccttcaat cgtttcacct
    16861 acttgacgta tttctttttc tgggaaactt tcccgtaata gttcggctaa tcctacttgt
    16921 gatcctaatg catcaggatc agggcgcaaa tgcctgtgga taatgatttt atcgtatttt
    16981 tttattgtat ctactatttc ttgctgtact gtcatttcac tcctgctttc taagaacgtt
    17041 ccattacttg acaaacaacg attgctttgg aaacgatggt gttttcaata aacacttcga
    17101 tatccagttt agcggaacgt ctgccgattt ccaatacacg tggacgaatg tctaattcgc
    17161 tttccaattg gatcagccgc aaataatgta ggttcacttg ttcaatcaag atatttcgtt
    17221 tttggttcat caccattgtt ttttgtgcga catcagaaat gatttcgcta agtacgccaa
    17281 aagaaatggt tccaacgcca ttgaccattt gtggtgctac tacgaatttg aacttagggt
    17341 tttccaaacg atttccttca cggtcagtat caatcgtatg gatatttcca gatatctgat
    17401 ccgaaatcgt atctgcaatc tgcggttgtc gctggacaag ctgcatcgct ttcattacat
    17461 cttgccgggt aatcaagcct tccaatgtca agtcatcgga aacgaccggc atgacttcta
    17521 gcccatccca gatcatttga tggctaacag aggcaatact cattgttttc tttacactga
    17581 ttggttcttt cgtcatcacc cgatcgatga tctggtttgg cgatttccct aaaacatctt
    17641 ttgccgtaac aatccccatc aaacgatgat ggcggttgac gactggaaaa cgcgaatgct
    17701 gcgtttcttc tgacaaacgc tgataatcag aaatcgtatc tgtctgctgc aaataatgag
    17761 tcttttcagc tggcatatag atatcactga caagcatgat atcttttttg atcaattggt
    17821 cgcttaacgc tcggttgatc atggttgcga ctgtaaatgt atcataagag gtgcgtaaga
    17881 tcggcatttc aagatcatca gctagctttg caatctcagc agtcgtattg aatcctcctg
    17941 tgatcaatac tgcggcgcca tcttccaatg ctaatttttg gacttccgcc cggttgccga
    18001 cgatcattag tgatcctggc gtaatatagc gttccatggc atgtttagtc atcgcgccga
    18061 tgacgaattt attcaagacc ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa
    18121 tgatcctgac gacctcgcca aatgtcaaac gttcaatatg tttttttaat ttgcgttcga
    18181 tacggatggt ccctactcgt tgaattgttg aaactagtcc gacattttct gcatctttga
    18241 ttgcacggta cgctgttcct tcactgacat tcaattcttt tgcgatgctt cttacagaaa
    18301 ttcgattgcc aacaggtaaa ctttcaatat attctaaaat caaatcatgt tttgtcgcca
    18361 ttttctgcct acacttccag cgtttccgag acgctcatta tttttccttc actatcttcc
    18421 agtaactcta caaaagattc gggatcttgt tcaatgacag ggaaagtatt gtaatggatt
    18481 ggaactactt tcttggcacg aattgtttgc gcagcctttg ctgcatcttc tggccccatc
    18541 gtatagttat cgccaatcgg caagaaagcg acatcaatat cgaattcttc tgcaagcaat
    18601 cctagatcac tgaaataagc agtatcccct gcatgataaa tcgtttttcc ttctgcttga
    18661 ataataattc cgctaggctc gcccatatac tgaacgatac cgtctacttc atagcttgaa
    18721 ctatgctgtg catgtacgaa ttttaccacg ccgaatggaa acgcatgttt tccaccgata
    18781 ttcataccga atgaatctac tcctctgctg ttcgcataat cagcaatctc aacaatactg
    18841 ataattgtgg cttgattttc ttttgcaata ggaatcatat ctcctatgtg gtcactatga
    18901 ccatgagtaa tcagcaacca atctgtcttt acttcttctg cagttaagtc tgtcaatgga
    18961 tttcctgtaa taaacgggtc aatcatcacg cgttggccat tttccatttc aattgttaaa
    19021 caggaatgtc catgatacgt aattttcata ttttacacct cgcgttttct ctgtatcttt
    19081 tttattaaaa acgatttcat cacttgcaca tcaatgaaaa aatatagctg ctaaatatat
    19141 tttcattgga ttgatagtac agtatgtaca aagcaattgt aacagataat acaggatgca
    19201 ccaagtacca agcagatata atctcaagtt tgaggtctct gctctgctta gcttctcttc
    19261 tcgtccctta actaattaaa tcagcgtaaa taggatggac gatcttttct aaatcggcta
    19321 cttttatccg aaggcttctc ccgatttcac cgcttgacac aattacttct tctttttctt
    19381 ttaaagaact gtctgctaaa aaacaataat caggatgtgc gagttttatt ccaatagggg
    19441 tgtttgctcc gtggggatat cctgtatgca ttaatacaaa atccattcca ggaaaaccaa
    19501 ctttacgatc tttcgtcact tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa
    19561 tcaatgcgat gatcacccca gttttgtttc ctttcactac gagagtttta catatcaaag
    19621 ctggatcaat ccctcttttt tgtagttctt ctgtgaaaga ttcttctacc acatgactaa
    19681 aatcaagtgg ttgatagctg atcttttgtt cttctaaata acgttcgact tttgtttgac
    19741 tcatcattga ttcactccct ttattctatc gtttattata aagcgaggga tgctgtattc
    19801 aaaaattatt tgtttttcgc aaaaaaagag caagacgagc actctataga gaggttcatc
    19861 ttactctttt tgtttatttt ttatttcgac gttgagtagc ttccataatg actggcaaga
    19921 tcactgggcg gcgttttgtt tgttcaaata agtaacggcc taattgatct cggatgtctt
    19981 gctttaattt gctccactca aaatcattgc tttctaaatg tttctcaacg attgtttcaa
    20041 tcatattgct gctttctttc atcagatcac ggctagcttt tacgtaaacg aatccccgtg
    20101 aagtaatttg aggttttgaa atgattttct tttctcgccg actgatagtg acaaccgcta
    20161 cgaaaatccc gtcttctgac aagatttttc gatcacgtaa aacaatgttt ccgatatcac
    20221 ctatgcctaa tccatcaatc attacatttt ctgcacttgt agttcctgca acactcatac
    20281 gcttgttctt atattccagc acgtctccgc gtccagtaat atagatattt ttgtatggca
    20341 tccctactgc atgagccaaa tctgcatgag cagctaattc acgatactct ccttgaactg
    20401 gcacaaaata tgttggtttc atcaaattga tcattaattg caagtcatta ggattggcat
    20461 ggccagaaac gcgcatattc tcagagatca acttcactgt tccaccagct cgataaatga
    20521 tatcttccgt tttcgccacg atggtttcca tcgcgatgct tggcgtagta gtgatataaa
    20581 ccaaatctcc ttcttcaatc ttgacattac ggtgtgtacc gtttgccatt ttttgcagag
    20641 acttgatggg ttcacccatt cggccagttt ctaaaatgat cagttcttct ggatcatagt
    20701 ttttcatgtc tttcaacgta acaagcagat cttcacttgg taattgaagt ttttctaaac
    20761 gcatcgctgt gcgaataatt cgttcgaaat cttgaccagt caagacaact tttcgttgtg
    20821 ctttttctgc tgcatccaat acttgttgca ctcgttgcaa attacttgct acacaagcga
    20881 cgatgatacg cccttcccaa tatttgatcg tatcaaatac ttcatctgct atttgggctt
    20941 cagaagctac aggctgagga ttttctgcat ttgccgattc acttagtaag gctaagactc
    21001 cttctttgcc gatttccgcc aatcgtgcat aatctgtttg atacattggg atagccgttt
    21061 ggtcaaattt aaaatctccc gtataaacga tactaccttc ttttgttttc agattgatcc
    21121 ccactgaatc tggtatcgta tgagtggtac ggaaaaagct cactactgtt tcaccaaagt
    21181 ctatttctgt atgctcatct accacatgga aatccttaaa tcctttggct gcttcattac
    21241 gactgacatt caattttgct aactcgatcg tcaactctgt cccgaatact ggtacagaaa
    21301 tttttgacaa caagtaagga agtgccccga tcgcatctgc atgtccatgt gttaaaaaga
    21361 caccagctac acgatcaatg ttttcttcta aatacgtaaa atcagggatt acgacgtcga
    21421 tccccaacaa ctcattttca ggatatttta gcccacaatc taaaataaaa atatcctcgc
    21481 ccacttctgc gacatacatg ttttttccat tttcacgaac gccgcctaat gggataattt
    21541 taatggtact cacaaacttc acctcttctt atgacatcca agtttaaaga tgtcgatttt
    21601 cttctttatc taaatatatg agccattcca atgaagcata tgcaaaatca ctatttctca
    21661 acttatggag aatcagcagg aaaattacca gcacatccgc gttctgacag ttggatgggc
    21721 attttatttt ggatatactt cattaacaaa atggatcttt tgcgaaacgc tatagccaga
    21781 atacttatat aagtattcag ctgttcccca ttattataca acaaataaac taaatcgcta
    21841 gtatcagcaa ttgttagaac aactatgcac gtaaaaatcc ctctgtagca tctaggattt
    21901 cttttgctat tcctctttca tttcttcttc gtcggtttga aacatacttt tttcatttta
    21961 ccacattttt tgcaataact atagtgactt tttatatata cagaacctca tgttgatggt
    22021 gtgcaaacag cataagattt aagctagctt taagataggt tgtctattat caaatgcatc
    22081 tactaggagg gatgcaaaat atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa
    22141 aatggggacg ttcattgtta ttgtttgccg tgttcacagc cattcttatt tttgtgctag
    22201 ctggcctcac gattcgaagc gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg
    22261 gagcaacagt gactttaagt gccaatcgag aagcttcatt taaaaaacag gaacaaactt
    22321 cttcaagcgg ttctaccact gaagaacgtc cagatccagg cagtttttct cttacaccag
    22381 tttccttaac ggacgcggaa aaaatagctg aattagataa tgtaaagagt tattcctttg
    22441 aaagttccgc ctcagctatg gcaaaagatg gaataactgc catcactagt tctgattcaa
    22501 gtgaagacag ttcagaaact actgatgcag gcggtgaacc tgctggagga atgggcggga
    22561 atccgcaaat gactcaagca gactttcaaa tcagtggtgt gtcagcaact gcacaaaata
    22621 gtaatttttc tgatggaaca gcaaaaatca cggatggtga aggaatagat gacagcgaca
    22681 aaggcacaaa caatgtagtc attgattcta ctctagcaga aacaaatgac ctggcagtcg
    22741 gagatacttt cacaatcacg agtactgaag atgaagatac cacttatgaa atgacgatca
    22801 aaggaattta tgaatccagc gaaacaagcg gcagtatggg tatgaaattc aacttcatga
    22861 atccgtcgaa tacactttac acttcttaca cttttgcaaa tgaactaaat gggacatctg
    22921 acgataacac gatagattct gctatttaca ctctatctga tccagataaa atggatgagt
    22981 ttctcaaaga agctgagaag ctgatcgaca cagatacatt tagcttacaa tcaaatgatt
    23041 ccatgtatca gtcaatgttg gaacctttaa acaacgtggc aagtttttct aaaaatgttg
    23101 ttttactagt ggctgtagcc ggtattatca tacttactct gatcattatg attaccattc
    23161 gggagcgtcg gcatgagtta ggcgtcttgc tatctttagg agaatccaga agtaaaatca
    23221 ttctccaatt gtttaccgaa gtggctattt gtatgattct tgcattagga gttgcttcgc
    23281 ttagcggaaa cgtcgtcgcg aatgccgtgg gacagcagct acttgaccaa cagacagaaa
    23341 ccacgacaca aaaccagcaa gctcctggcg gtggacaaat gccaggtaac agacaagaac
    23401 aaggcggtca acgcgatggt caacaaggaa ccacgaataa tccatttgct gtttctgatc
    23461 aagtcagcga attggaaatc aatgtcaaac ctgcacaatt gggactatta gccggagtag
    23521 catttggaat cagtctgttt tctgtctttt tagcttctat agggatatta agattaaacc
    23581 caagaaaaat cctattaaac taaaggagga atttgatgtt acggacagaa caattgggat
    23641 tttggtatca agatccgcaa gatgcgcttt ataaagatgt agaactgaat ttcgaagctg
    23701 gaaaaatgta cgctatttta ggagcaagtg gttctggaaa aacgactttt ctttcgctta
    23761 tttctggact agatgcacca aaagaaggaa aggtatacta tcaagatcgt tcactgaaca
    23821 aaatcgggtt aagaagttat cgtaaaaatg atgtctcgat tattttccaa gcttacaact
    23881 tgcttcctta tttatcggct ctagacaacg tcatagcagc aatggaaatc actaagacca
    23941 agcaagagaa ttatcgacaa gttgctttag atcgactggc tcgtgtagga attgatgaaa
    24001 tattagcaaa aaaacaggtg accaaacttt cgggaggtca gcagcagcga gtcgctatcg
    24061 ttcgtgctat ctgctgtgaa catcaactaa tcgtagcgga tgagcctact ggaaatctag
    24121 atgaaacaac ttcacaagat atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc
    24181 gctgtattat catcgtgact catgagattg aagtagcaaa aagctgtgat gaagtctttg
    24241 aactaaaaaa taaaaaattt caaaaaatcg aagtttaaaa aacaggataa tgatcaaata
    24301 aaaaatccga accatatctg tctcgcagat atggttcgga ttttttattt gcttgattca
    24361 attagtaata agtggttttt attttttttc gtcttttcga gaaaacttgt ctttgacttc
    24421 gtcgcctaaa tttcgagctt tttctttgat gtcagcacct agtgattgtg ctttcccttc
    24481 tgtttctttg ctcttgtcgt ctgttgcttt gccgtatgct tcttttcctt caccgactac
    24541 tttatctttc atatctttag aacgtccttt taaatctgcc attgtgcttc ctcctcttag
    24601 tttgtttatt gatctacctt cagggtaaca tcaacaacag atacagacaa acagaacgct
    24661 cgttttctaa aaaagatagt tagagctgtt gattaaaaac ttttttcgaa agtcccccat
    24721 actttgactt ctttttcttg gaccattatt tctcctttag cgataactgt atcaatatca
    24781 agcgtttctt tatccaagat acacagatct gcatcagcac caatttcgat ttttcctttt
    24841 ttatccaatt tcaatatacg tgctggatta ctggttatag ctcgtaaggc gatttccaaa
    24901 gggatacttt ctttttgcac agcttctttg atccctacta gcaatgcctt ggcacttcct
    24961 actcctaacc ctaggaaatg attgttttca tcaaaataag gcaagcttcc ttgtccatct
    25021 gaggtcatcg tgaaattatc taaagaaaca tcttcctcga tcaatcgttt cagtgctttg
    25081 ctgaagcgaa cttcaccatc tgtcttctcc caaaaatcag gatcttcgct agcagtgaag
    25141 tcaatcgtgc caccacgttt ggcaaatgcc acgcaagctt caaataattc tggtgtgcga
    25201 tttgcatgag taggcaaaaa ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc
    25261 aatagctcta gctttctttt tcctccgcct aagtggacat tgatgatccc agcttttccg
    25321 gataacatgc ctcctacacg agcatctgct actgcacgag cgaactcttc aaaacttggc
    25381 tgagaagaac ggtgatcgga tactgcgatt tctccgattc cgataatttt atcaattgtc
    25441 aaaaagtctt tgatgataga atctgttact gtttttaccg gcaatcggta cgaaccttcg
    25501 tatacataag tggagattcc ttctgcttcc agacctttcg ctttgctgat caacgcagtc
    25561 atatctctac cttctccgtc tgttcccaaa caaccgacaa ctgtcgttac accagctgta
    25621 gtcaattggc tcaatgtgac ttctggcgtt cgattttgat aaccgccttc tcctcctcca
    25681 cctaaaatat ggaaatgaca gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa
    25741 agttcttcta cttccactcc ttcgtagccg ccttctagct gttcttctac agccaaaatc
    25801 ttcccaccgg caatcagtac atccattttt cctaagtaat ctggtgcaaa cacttcaatt
    25861 tgtcgaatga ttttcataca tttttcctct ttctttaagt ataactgatg ataggatctt
    25921 aaaaaataat ccaaacgata ggatctttac tcctttgatt ttaccgtaaa atgacggttc
    25981 ttggagtcct tatctactgt ttggattact tttttattta caggtctcca gtactactgc
    26041 agacctattg caatggcaat catgatgaaa atagttccta aaacaaacag gaatccttgc
    26101 atcttgattt ggaatcttgc ccattttgcc cattcgatcc gtgcaacacc tagcacacca
    26161 atcaagctgg ctgatacggg tgtaaaagcg tccataaaac ctgaaccaag ctgataagca
    26221 agaactgcta cttgtctgga tacacctact aaatctgcca gtggagccat gattggcata
    26281 gtcaaagctg cttgtcctga atttgatgta acaaccaagt tgaacaaact ttggaagatg
    26341 tacatcgccc aagcaccaat gacagctgga acaccggtca acgccgtccc gatcccatgc
    26401 aaaatcgtat tcaaagctga aggaacagat gcatcagatc ctcccaagac aagaagaatc
    26461 cctttggcca tacctactac gattgctgta ccagctaaat cagctgcacc agattggaaa
    26521 gaagaagcga tgtcattgac tttcattcca tctaatttga aaagaacagc aatgattcca
    26581 gcaactaatc ccatcacaaa gaattgagaa gcgatttctg gaatgtaata gcctttttga
    26641 gtcacgcccc aaacgatcca gacaaggaca actaacatct ctaataagat caatttatgc
    26701 ccaagcatga attttttatt ttcatcagat gtctttttga tatgagaacg gaaataatca
    26761 tctgatttgt aagtcaacga actttctggt tttttacgga ttttttctgc atatatcatc
    26821 atataaccag cggctaatgc agtaacaact acccacataa tcaatcggaa tgtcgcacca
    26881 gacaacacgg ggattccagc aatcccttga gccaccgcta cgctaaacgg gctcatccaa
    26941 gaggtcgcat ttccgacttg agatgcgaca taagtcacgg ttactgctac gatcgagtca
    27001 tatcctaacg caatgacaaa tggaaccatc accattgaga atggaattac ctcttctgcc
    27061 atcccgaatg tagcgccacc gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc
    27121 tccagtccct tagttttact gataaacgca tagatacctg cgtcgatcgc tccggtacgc
    27181 ataataatac caaaagcacc tccaacaaca aggatcaatg cagcgatccc tacagcagat
    27241 ccatacttat ctccagatac tagtccttca aaaacaaaat ttaagaaacc aaagccgcca
    27301 aaatcatctg ttccccatat tttggctgtt ttatgcaatt ttttagaagt atcatagata
    27361 ttttcgccgt actgatcgta aagcacatca tctgtcaagg agatttcatc tagtttttcc
    27421 tgcgtaagat ttttttctcc gtcagcaatg actttttcca atcctttttc cggaacatcc
    27481 agtttttcac gttctgctgg atgatcttgc agttcttcta attgatcaaa cacatacgac
    27541 ttatccagct cgtaagcata acggaaggaa tcttgacgta aaacagtacg agtacttgtt
    27601 tcgccatttg catctttgta ttcaatatct tgcgtgctga actttcctgc tggaacgaca
    27661 aaagtcagta tccatgcaaa gatgactaca ccaaaaataa tgacatacgt atggggtgtc
    27721 gataatttag tgaagtcgcg tttcggtttt cctgaagaat ttgttttctt caaatttatt
    27781 tcttcctttc ttttttcagt aattttgatg aataa
//
LOCUS       DAYYCM010000034        27292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000034 DAYYCM010000000
VERSION     DAYYCM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.035"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1629
                     /locus_tag="ACHAY1_001516"
     CDS             151..1629
                     /locus_tag="ACHAY1_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593517.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1906..3966
                     /locus_tag="ACHAY1_001517"
     CDS             1906..3966
                     /locus_tag="ACHAY1_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8593518.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4049..4618
                     /locus_tag="ACHAY1_001518"
     CDS             4049..4618
                     /locus_tag="ACHAY1_001518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593519.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4793..5599)
                     /locus_tag="ACHAY1_001519"
     CDS             complement(4793..5599)
                     /locus_tag="ACHAY1_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593520.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5737..7251
                     /locus_tag="ACHAY1_001520"
     CDS             5737..7251
                     /locus_tag="ACHAY1_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8593521.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7654..8637)
                     /locus_tag="ACHAY1_001521"
     CDS             complement(7654..8637)
                     /locus_tag="ACHAY1_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8593522.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8642..9289)
                     /locus_tag="ACHAY1_001522"
     CDS             complement(8642..9289)
                     /locus_tag="ACHAY1_001522"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8593523.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9374..10006)
                     /locus_tag="ACHAY1_001523"
     CDS             complement(9374..10006)
                     /locus_tag="ACHAY1_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8593524.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10022..10573)
                     /gene="coaC"
                     /locus_tag="ACHAY1_001524"
     CDS             complement(10022..10573)
                     /gene="coaC"
                     /locus_tag="ACHAY1_001524"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8593525.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10570..11307)
                     /locus_tag="ACHAY1_001525"
     CDS             complement(10570..11307)
                     /locus_tag="ACHAY1_001525"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8593526.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11304..12293)
                     /locus_tag="ACHAY1_001526"
     CDS             complement(11304..12293)
                     /locus_tag="ACHAY1_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8593527.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12551..13093
                     /locus_tag="ACHAY1_001527"
     CDS             12551..13093
                     /locus_tag="ACHAY1_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8593528.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13110..14195
                     /locus_tag="ACHAY1_001528"
     CDS             13110..14195
                     /locus_tag="ACHAY1_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593529.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14207..15013
                     /locus_tag="ACHAY1_001529"
     CDS             14207..15013
                     /locus_tag="ACHAY1_001529"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593530.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15014..15841
                     /locus_tag="ACHAY1_001530"
     CDS             15014..15841
                     /locus_tag="ACHAY1_001530"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593531.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15838..16911
                     /locus_tag="ACHAY1_001531"
     CDS             15838..16911
                     /locus_tag="ACHAY1_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593532.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17339..20035
                     /locus_tag="ACHAY1_001532"
                     /pseudo
     CDS             17339..20035
                     /locus_tag="ACHAY1_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            20100..20600
                     /locus_tag="ACHAY1_001533"
     CDS             20100..20600
                     /locus_tag="ACHAY1_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593533.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20612..21106
                     /locus_tag="ACHAY1_001534"
     CDS             20612..21106
                     /locus_tag="ACHAY1_001534"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8593534.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21127..21936
                     /locus_tag="ACHAY1_001535"
     CDS             21127..21936
                     /locus_tag="ACHAY1_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8593535.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            21933..22754
                     /locus_tag="ACHAY1_001536"
     CDS             21933..22754
                     /locus_tag="ACHAY1_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8593536.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22805..24904
                     /locus_tag="ACHAY1_001537"
     CDS             22805..24904
                     /locus_tag="ACHAY1_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8593537.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24901..26019
                     /locus_tag="ACHAY1_001538"
     CDS             24901..26019
                     /locus_tag="ACHAY1_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8593538.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26058..26936
                     /locus_tag="ACHAY1_001539"
     CDS             26058..26936
                     /locus_tag="ACHAY1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8593539.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27082..27279
                     /locus_tag="ACHAY1_001540"
     CDS             27082..27279
                     /locus_tag="ACHAY1_001540"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8593540.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8715 a         4685 c         5717 g         8175 t
ORIGIN      
        1 tttttgcaat attctcaaaa aaaagataaa aaatgagaat aactgatgaa aaaacaagca
       61 taatccatca taaaatcagt ataggtgaaa agatgaactt tcatctgcat atcaagtaat
      121 tggtatgagg cattttttga ggtgtaggat atgaagttgg attaccttat agaagaggaa
      181 gatagagaaa aactgctgct agctagatat ttagaatttg agaaaaagaa ttatgtagtg
      241 ctgcctaagt tatatgaaac attaggtttg tctaaattta aaacaagaaa ttatatcata
      301 gaattaaata aagaatttac tgaattcaaa acaaagccag agatccaaat ttcagaagaa
      361 gcagagatcc atgtcgaacg tctgaatttg acggatatca aaaatttaag aagaatctac
      421 tttcaacgat caaaaattgc tcgactgtta acggagatcg tggattcaaa agtaacgatc
      481 gaaaaatttg cagagaatca ttttttaagc atatcaagag cctatgtaaa aagaagagaa
      541 ttagtctctt ttcttgaaca gcatcaaata aagttaaaga aaaacatatt agttggggaa
      601 gaaataaaca tacggaacgt attatttggg gtatatttcg gggtctttaa tggtttttct
      661 atgccattta gagaaagcgt catgaatgaa gcaaagagta tcgtgcaata ttttgaatat
      721 ctgttcaatt taaaactgtc tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt
      781 agtttgttga gggcctatgg gaatcaacca atcggaaaac gtttcttttc agatgaatac
      841 tttaagagct cagaaggaat gaagatcatc aaggatttat cacatatttc ttttcttcat
      901 atccctaaag agcagatcat tgatgagatt tcttatatca tgttgttttt gatcacggaa
      961 gaagaatttg aattagatgg cgcatttgga cttgcggaag ctcattttca tgatatccac
     1021 atgattagtc aaaaaatgtc agtggaattg attgatcaat taccattttc gattgaaatc
     1081 tctgaagaag aaagactata tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat
     1141 cgaaaaaatt ggatattcga ctttgagatg actagcttta cgtctaaaaa gcagctacag
     1201 tttttcttcg agacgtatcc aatattcagt acagcaatcc aaaaggtcat acaagagaat
     1261 aaacgctatt tcttgggaaa agatgaacgt ttcatgattc gcttatttta tgattatctc
     1321 tttttattgg tcagtatttg tccggtatca tattttgaag caccgattca tgtctgtatt
     1381 gatttttcgc aaggatctgc ttataccgat tacattattt ctcaagtgaa aggatttaaa
     1441 aattacaata ttgtgattga gagtcgaatc acttctcgta cgaatattta tttatctgat
     1501 tgtttacttg aaaaatttaa taaagaacag ataatttgga aaaatccacc gactccagat
     1561 gactgggaat attttggaaa taccatcatc agagaaaaaa atagacattt aactgaaaaa
     1621 atcagctagt atttatggat ggcagctttg tctcacatat ttattagctg atttatgttt
     1681 ttttcaaaga aaaggtaact agatataact agatataact agatatatta cttaacaaaa
     1741 ttaaaaagta cataagttat tattccgatt ttcttgaaac aataaaaaat caaaaaaaat
     1801 actctcctaa tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa
     1861 attactactt catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga
     1921 cgttcaaaac aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa
     1981 aaacactacg ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat
     2041 atgcatgtac ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct
     2101 gagaccactg attcccctat cactattact catactttca ctgatgcaaa tggaaaacag
     2161 ctacttcctg cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac
     2221 tggttaattc ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct
     2281 atcttttccg atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt
     2341 ttgaatcatg ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat
     2401 gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt
     2461 tcgatatctg atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc
     2521 aaagtaattt caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt
     2581 tccttgtcct attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat
     2641 gttcgtgctg atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt
     2701 tacgccccta aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa
     2761 gaagaaatca aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta
     2821 ttaacttata tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta
     2881 gaagcatctg ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa
     2941 gaacttcaag cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct
     3001 gctgaatttt ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa
     3061 gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg
     3121 gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa
     3181 attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt
     3241 gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta
     3301 ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat
     3361 ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat
     3421 tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata
     3481 gatcaaaaag aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca acaaatggct
     3541 catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata
     3601 tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa
     3661 gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa
     3721 tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa
     3781 aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct
     3841 agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg
     3901 cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg
     3961 ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg
     4021 cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat
     4081 ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga
     4141 atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg
     4201 ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc
     4261 tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac
     4321 ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc
     4381 agtgaaatca gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca
     4441 agaatttcct accctccata cggacggacc aatctatctt cacacacaga catggagcca
     4501 tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg
     4561 cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg
     4621 aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct
     4681 tacttttttg agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag
     4741 gggcagtgac aaaagtattg tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt
     4801 tgattttttt aggaataaaa ttcttgatga acgtaaattt ttggtaatcg tttcgactat
     4861 agaggtaacc aaatacactg aaaacaactg ctccaataaa attaacaaat aaatccttca
     4921 tagtatccat gattcccacg tctaagtaac cattttggat gattgtttta tctgttttac
     4981 catctgcatt ttgactttca atgattgtcc gagtgatatc tggaacatga tagacagtgt
     5041 ttccgtcttt acttaactcc acactattga tttgctggat gatcgtatct ttttgcatat
     5101 cgaattgcag taatttatct gctgaaaatt ccacaaactc ccacataaca ccaactgtca
     5161 tcgaaaaaca gaaggaaaca actgctagta ataaaggagt caagttcatg gtttttgtat
     5221 ttttattgag caaataaaca agggaaaagc cgacaccccc agctaaaaat ccgctgaatg
     5281 catgtagaaa agtatcccac attgaaaaat agccataaaa atcacttaac tctcctaaaa
     5341 ctgttgaact aaaaataaaa gataacaaaa tgatttctag catatttgga atctgtatct
     5401 ttaatatctt ttccagaaaa taagggattt caaataaaat taatgtcagt aataaaagca
     5461 ttgtatttcc ccagtgttgg ttccagacct gatccactag cataataacg acgatgatac
     5521 gtaataaaat atagattccg tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa
     5581 aggttttcaa atgattcata aaataactcc ttcatgtata tccttaatta tactaagaag
     5641 cgtgtgttta ccaaagctat atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat
     5701 cacttaatat agtataagga gaaaaggggg atacaaatga aaaaattagt tgctggttta
     5761 ttaacagccg ttgcacttct tgctatgagc gcatgcggcg cagatcagaa aaatacagcg
     5821 cagacaagtt ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc tgaacaagta
     5881 tatacagatc caaaagaaat gaaagaatct tatgatgtcg tgattattgg agctggtggt
     5941 gcagggatgg cagcagctat cgaagcaaaa gatgctgggt tgaatccagt aattttggaa
     6001 aagatgcctg ttgccggagg aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa
     6061 acaaaatttc aaaaagaaga aggaatcaaa gactcgaatg atgccttcta tgaagaaacg
     6121 ctaaaaggtg gtggaggaac gaatgataaa gaaatgcttc gatactttgt cgatcactct
     6181 gctgatgcgg tcgattggct agatcaaaac ggtatcaaac tggacaactt aacgatcact
     6241 ggagggatga gtgaaaagag gacacatcgt ccgtctgatg gctcggctat tggtggatac
     6301 ttagtggagg ggttattgaa taacgtccat gagcgggaaa tccctatttt tgtcaacgca
     6361 gatgtgacag acattaagaa aaatggggaa acggtcaatg aagtgactgt acatgttcaa
     6421 ggagaagaac caaaagaggt caccggaaaa gcaatcgtag ttaccacagg cggttttggt
     6481 gccagcaaag aatttattga aaaatatcgt cccgatttag ctgattatat tacgacaaat
     6541 caagaaggat caactggaga tggtatcacc atgattgaaa aattaggtgg acaaaccgtt
     6601 gatatggata aaatacaaat tcatccaact gtgcagcagg atgagggggt cttgattggt
     6661 gaggttgttc gcggagaagg agcaatcttg gtcgatcaag aggggaaaag atttgtcaat
     6721 gaaatggata caagggataa agtatctgca gcaattacag cattgcctga aaaatcagct
     6781 tatttgattt ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa
     6841 aaaggatatg tcacaactgg aaagacaatg gaagaattag cagaaaaaat cgatttacca
     6901 aaagaaacac tagcaaaaac agttgatgga tggaatacgg atgttgcgga gaaaaaagat
     6961 gctcaattca accgtgccac agccatggaa catgacttat cgtctccgaa ttattatgcg
     7021 atcaaaatcg caccaggaat ccattacacg atgggcggtg tgaaaatcaa tacgaacact
     7081 gaagtattag ataaagaaaa ccagccaatt agggggttat atgctgctgg tgaagtcaca
     7141 ggtggcttac atggaaacaa ccggatcggt ggaaattcag ttgcggagat cattattttt
     7201 gggcgacaag cgggcataca agcagctcga tttgcttcgg aaaatgaatg aaatagaacg
     7261 taaataaaaa gaggttgtga aatcagcgtt ttgagctaat accgagggtc agctgattga
     7321 acactgttta gtgaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
     7381 agtaaaaaca gcttcttatg tttttaactt ggttggactt attaccgcag atgcaagctt
     7441 ttgaacaccg tttattcgga aaaagagcgt gggacaaaag taaagaacac ttttgtctca
     7501 cgctctaaac acgtataaac ggcggaagca gaagaaattc cttcggaaat aagctgaaat
     7561 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     7621 tctgtcccag cctctttttt gatcagcatg attttattca gttatcttca aaatgatttt
     7681 tccgatgttt ttgtttgctt ccatatattc atgcgcttgt ttgactgcgc tgaattggaa
     7741 tgtacggtca atgattggcc gaatcttgcc attttctaga aaaggcccag ctttttgcat
     7801 aaaagtttgt gcgagctctg ctttatactg atcacttcta ggtgtcaata atgtaccaag
     7861 cagttggaca cgtttcccaa tcaatacacc aaggtctatc tcaggcacga tggtcccacc
     7921 tagcatacca atcaagaccc aacgtccatc aacggctata gaagctaaat tcttttccca
     7981 gtaagaagcg ccgatgaaat ccaaaataac atcgactccc cgaccttcag tgatttttcg
     8041 tacctcttct tgaaagtctt gttttttata gttgatcaat tcatctgcac caagttcttt
     8101 acaaaaggcg agtttttctt ctgatcctgc agtaacgatg attttcgcgt gagaaagctg
     8161 tctagctaat tggatcgctg ctgttcccac accacttgct cctgcatgga tcaagacagt
     8221 ttcgccttct tgtaattttc caagccagta aagtgtttga taagcagtca aaaaaacttc
     8281 tgagattcca gcagcagaaa catagtctag cgtatcaggg agaaggattg cacgatcggc
     8341 aggcatcaca gcatattcgg cataaccacc tagattgact aatccgtata cttttgtccc
     8401 aggagtaaaa gtggagatat cacttttatt ctcaactacc tctccagcta cttcgactcc
     8461 caaaataggg tagggaggtt tcaaagagac tgattctctt ttcataatat ctgttcgatt
     8521 gacagctgct gtatggactt tgatcagtaa ttcgccttgt tttggtttag gaacaggcac
     8581 ttcaatttgt tctaatacat ctacagaacc aggatgcttg attgaaatgg cacgcatgga
     8641 tctattcctc ctttgcccat tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa
     8701 gtactagagt cgtacgagaa atatccgttt cttccactag agcggcaagt acagcagcaa
     8761 ctgtagagcg agtgatcgtt tttgatgcct tctcattagg cttagttaat tgaatggaag
     8821 taacttcttc atcgttagtc aatgtgactg gttgaagaat gacaaaatct aagttagttc
     8881 gtttcagcca ttcatctgca taatgtttag taatataata atctgtcatg gactcaggcc
     8941 attttgctgg ttcatctgca taaacagcac tgaccatcag atagcgagag acatgcgcat
     9001 tttcagctgc gatcactgtt ttgattgcgc catccaagtc tacttgcaga agattctttc
     9061 cttgagagcc ggcagcaaaa atcaatacat cgatgccttt gaatgcttcg gtcatttttt
     9121 ccacggacca agttaaatca aaagagacat acgaaacatt ctcttttttt actaatgttt
     9181 gggaagcaac atctctcacc cctgcaaaaa tttcatggga acttggagct aaatctttta
     9241 tcaggtgtcg gccaatttgt ccgtttgcac ctacaacaaa aattttcatt tggatcatcc
     9301 tttcgacgtc aaatctttcc attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg
     9361 gctacgatga ctatcagtta gtaagagaag ggtgcttgaa gtatcttaac aagacattgc
     9421 atactgctgc agtcgcaaga cctgcgacga ttccttcagg tataccactt gtaaaaataa
     9481 ccgctagaat agctgaataa acagcattta aatttgttcc gatgactttg gcataatctt
     9541 gctggaataa aaagtagatc atattcatca ctaaaatcgt attggtcaat gaaccggaaa
     9601 gtccagctaa gaataaagaa acggaatctg ctttttgttt cattagtttg cgcagacctt
     9661 tataaacgaa ataaggaacc accccaatca agatgcgagg aataaacgcg atcagcaagg
     9721 ctttccagct tccatgttct gttccgatga ctgggataaa cggagaaaag acaaaagaca
     9781 aaggtgtttg gataactgtg ctacggatca tgctgatcag tccaaatgta ccgccaagaa
     9841 aagcacctag tcttggtcct aaaacgatcg aagcaatgat tacaggaata tgcatggttg
     9901 ttgcattgat tggaccgata gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa
     9961 tagctaaaaa catcgcagtt aatgtaaagt tttttgtatt tttcataagt actccttttt
    10021 tctaagcatt tctttcatta agagtcagca tgacctgttc gatgatcgtc tgaatatcag
    10081 ctaaggcacc ttttccaaaa tctccacaag ctaaaagaga ttctcttggt tcaatttcct
    10141 gatagccgac agatctcaat gtttccagat tacgctgagt gatcgggttt tgatacatat
    10201 tcgtgttcat ggcaggggca atcaattttg gtgttcccac tggaagcgcc attgcgactg
    10261 ttgaaagaag atcgtctgca atgccgtttg ctagcttgcc gataatgttt gctgtagcag
    10321 gcgcgatgag aaacaagtca gcttgttttg ctagttcgat atgattgatt acctgcggat
    10381 ttgattcttg catgacgtct gtatggactg cgcgcttcga cagagattgc aaggttaatg
    10441 gcgtgataaa atgtgtgctg cttttagaca taatcacatc tacctgggca cctaatttga
    10501 cgaattgact tgtaagatca gcgcttttgt aagcagaaat acttccagta acgcctagta
    10561 agatcttttt cattttgatt cctccagtct aattttctca gtaatcaatg cagcaatctc
    10621 agatttactt tgagcttctt ccactgttcc atctttagat aagagatagc cgtggtgtgt
    10681 ctcgccatgg atttccgtca aatcatttgc gaaaataaaa tcagttttgt tatttatcag
    10741 actatttttt gcgactgcca gaagagattc ctttggtaca tctactaata gtttgaaacc
    10801 aatgattttt gcttttggct gccattcacg tagataagaa atgatttttg gtgttttctt
    10861 caaaacgagc acaagatgct cggtgttaga agagatcttt gaaacagtgg tatctgtctg
    10921 ttcattttcc gcaaaccaat tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt
    10981 tgccagctgt tcttctgaaa aactaaaagc aggagtaaaa tcactgactg ccatactgtg
    11041 gataatcgca tcgtagtgtt cttttttcgt tagttcttct aattgtaaaa ataagtccag
    11101 tgcagattcg atcgtataaa tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt
    11161 cgtagtcacg taatcgattg tggtatccgg attggcagct aaataatcag ctaatgcttg
    11221 ccctaatcgt cctgtcgaat gatttgtaat agcacgtact tgatcgattt tttcagtcgt
    11281 tcctccagct gtgattagaa tcttcattta tggtcaccta catttactag tgttcttcct
    11341 tggtgtgttc cagcaagtag tttagatgct gtttcaggca attgatccag agtaatctct
    11401 tggacgacca gttcatctgc aatctgcaaa tccgccatct gttgccataa agaaagacgt
    11461 tgatctattg gtacattgac agaatcaatt ccaatcaact ggatgtttcg cagaataaaa
    11521 ggcaagacag tcgcgtttag tttgattccc ccagcattgc cacataaagc tactgcaccg
    11581 ttataagaga tcaatggaag aagactgctt agctgctcgc cacctactgt gtcgatgaca
    11641 tagtcgattt gctgttttcc taaagctttt gttgtctctg gcagaaattc atcaggtcgt
    11701 acgacttgca aagcacctag actttttagc catgtgacag actcttcttt tctggaaaag
    11761 gcaatgattg actggaatcc tagtttatgt aacagggcta tcgctgtact gcctacaccg
    11821 ccagaagcac cagtaacgac aactttttta tcggaggtca taccttgacg gattaaagcg
    11881 ttcacacaca acatagcagt aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc
    11941 atattttttg gtagaggaac taaccagtct cctggtactt tttgatattg gctataaccg
    12001 cctggatggg tgacgccaag cccaaaacca gtagcaagta caagatcttc ttttgaaaaa
    12061 cgtggattgt tactttggac gacagttccg gataaatcaa tccctggagt catcggatat
    12121 gttcgtatca cctgaccaga ctcgctcatt gccaatgcat ctttataatt gacatcagaa
    12181 taggcaattt ttaccaagac ttcattttca gccaactcag taaatggcag atcactgatt
    12241 gctgtatgga aaacaggttc ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag
    12301 acactccttt taaaattttc ttttatcata ccataaagcc tactctttta ttcttgttct
    12361 tccgtaaatt tagacaaaaa ttaggtgaaa aataaagaat caaacaacta gaatttattc
    12421 caaaagataa agaaatattc agcaaaaaaa attttacaaa gggttgcctt tttcaataaa
    12481 tttcgtatac taatacgagt ggtagtaaac aggaagttta ctattagaaa acttcgattc
    12541 ggaggaaatt atggaaattg gtgagaagtt aagaaatttg cgcatccaaa agaatttgac
    12601 acaagaagaa ctaggcgaac gcacagattt gtcaaaaggg tatatttccc agttggaacg
    12661 agatttaagt tcaccatcaa tggaaacatt cttttctatt cttgaagtct taggtgtgac
    12721 tccagaagaa ttttttcatc aagaagcagc gaatctgcaa gtggtttatt caaaagaaga
    12781 tcatacggtt tatttcgatg aagaaaacgg gtatgaattg gaatggctcg tggctgattc
    12841 gaatgaaaaa gaaatggagc ctgtcttgct gacttttgaa ggtgcaggag aatataagac
    12901 gtttgaacct tcattatctg aaacgtttat ttttgtatta gaaggggaat tagagttaag
    12961 cttaggagag acggtctatg tggcaaaagc aggacagtcg atctattatc aagcgacaaa
    13021 acaacatcaa ttaaggaatc atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca
    13081 atcatatttg taatataggg ggtaaaaacg tggggaaacc aatcatttca tttgatcaag
    13141 tagtgaaacg ctatgatgat gaaacagtat taaaaaaagt aagttttgag atcgaacaag
    13201 gaaaatttta tacactgctt ggtccttccg gctgtggtaa aacaacgatc ctacgtatta
    13261 tagctggatt caccgaagct acagaagggg acatctattt tgaagggaaa cggttgaatg
    13321 atttgccagc taataagcga caagtaaata cagtcttcca agattatgca ctttttcctc
    13381 atatgaatgt ttttgataac gttgcttttg gattgaagat caagaaaatg tccaaatcag
    13441 atatcgaaaa gaaggtaaaa gatgctttgc gtatggtcca gcttcctgga tacgaaaaac
    13501 gggagattag tgaaatgtct ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg
    13561 taaatgaacc gaaagtattg ctattagatg aaccgttatc ggcgcttgat ttgaaactac
    13621 gtacagatat gcagtatgaa ctgcgggatc tgcaacaacg attaggaatc acgtttattt
    13681 ttgttactca tgaccaagaa gaagcgttgg caatgagtga tgagattttt gtcatgaata
    13741 aagggaaaat cgtccaaagt ggtacacccg tagatattta tgatgagcca atcaatcatt
    13801 tcgttgctga ttttgttggg gagagcaata tcgtggacgg tgtaatgatc gaagataatc
    13861 tagttgagtt tgttggtaag caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac
    13921 cagttgaagt tgttttacgc ccagaggatt tgacgatcac gacaccagat aaaggaaaac
    13981 tagtcgtaac ggtcgatact cagctgtttc gtggtgtcca ttatgaaatc atctgttatg
    14041 atgaacagca aaatgaatgg atggtccatt caacgaaaaa agctaaagaa ggaagcaaag
    14101 taggattggc ctttgagcca gaagatatcc atgtcatgcg tttcaacgag tctgaagaag
    14161 aattcgatgc acgtcttgac agctacgaag aatagggggg acaagaatga aaacaatgcg
    14221 caggatctat tccgtccctt atattttgtg gctcggttta ttcgtgatcg cgcctgtatt
    14281 aatgatcatt tatcaatctt tttttgatat gaatgggcaa ttcacgctga ataattatct
    14341 atcttatttt acgtcgggga catcgctttc tatgacgatc aattctgtat ggtatgcatt
    14401 tttgatcacg tttttcacgc tgatcatcag ttacccgact gcttattttt taacaaaact
    14461 gaagcacaag caattatggc tgatgctgat tattttgcct acatgggtga atctgctgct
    14521 taaagcttac gcatttattg ggattttcag tgtgcacggc agcgtgaatc atttctttga
    14581 ttttatcggg atcgggccga aacagatatt attcacagac ttcagttttc tgttcgtggc
    14641 aacgtatatc gagattccat ttatgattat gccaattttc aatgcattgg aagagatgaa
    14701 tccttctttg atcagtgcta gtagagattt aggcgcgaat agctttgaga ccttccgacg
    14761 agtgattttt ccgttatcat taaatggtgt gaaaagtggt gtccaagcag tatttattcc
    14821 ttctctttct ttattcatgc tgactcgtct gattggcgga aatcgggtga tcactttagg
    14881 aacggctatc gaacagcatt tcttggttac acaaaactgg gggatgggtt ctacaatcgg
    14941 tgtgatttta atcattgcta tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg
    15001 aggtcgctca taaatgaaaa aatttaaatg gtcgtatctt tatttaggga tcgtcttttt
    15061 attcctatat gcaccgatct tctatctgat tttttattcc ttcaatgaag gaggaacgat
    15121 gaacagcttt acaggattta cttgggaaaa ttatgaagct gtttttgaag atacacgttt
    15181 gatcattatc gtattgaata cgttcatgct ggctttctta tctgcattga tcgccacgat
    15241 catcgggaca tttggggcta tgggaatcta ttacacgaaa aaacgacgtg cacggacagc
    15301 gttgttgagc atgaataaca tcttattagt ctctcctgat gttattatcg gtgctagttt
    15361 cttgattttc tttacactac taggattcgg attaggtttt cagagtgtat tgctttccca
    15421 tattgctttc agcattccaa tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa
    15481 cgattcaatg gtcgatgcgg ctagagacct tggtgctaat aatttccaag tagtcaaaaa
    15541 tatcattttg ccttatcttt ctccaggaat catagctgga tattttatgg catttaccta
    15601 ttctcttgat gattttgcgg ttacattttt cgtcacagga aatggcttta cgacgctctc
    15661 agtagagatc tattctcgtg caagacaagg aatcagttta gaaatcaatg cgctaagtgc
    15721 gttagttttc ttgttctcca tgatattggt cattggatac tactttatta gtaaagaaaa
    15781 ccgtccaaaa cggctgaaga aaaacagaag agtacgcgag ggggtggcac ggctgcgatg
    15841 aaaaaattac aatctctgtt tttaggtatt ctcatcatta ttgcggtgct gctgcttggt
    15901 gttcgtcaac ttgaaaaaac aagtggcatg agtggagcaa aggtacttac catctataac
    15961 tggggagatt atatcgatcc tagtttgatc acgaaatttg aaaaagaata tggatacaaa
    16021 gtgaattatg aaacatttga ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg
    16081 acaaactacg acatcgctat accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa
    16141 ctagtacttc cattagatca ttcgaagatc aaaggattaa ataatattga tccacgattt
    16201 ttagacttgg attttgatcc agataatcag tattcgattc catatttttg gggaacttta
    16261 gggatcgtat ataacgataa attctttgat gcaaatcaaa ttaagcattg ggatgatttg
    16321 tggaagccgg aattaaaaga cagtttgatg ctgattgacg gtgcacgaga agttatgggg
    16381 ctttctttga atagtttagg ctattctttg aatagtaaga atatggcaca gctgacagaa
    16441 gcagcaaata aattgaatca tttaacacca aatgtcaaag caatcgtggc agatgagatc
    16501 aaaatgtata tgatcaatga agaagcctcc gtggcagtga cattttctgg cgaagctgcc
    16561 gatatgatgt gggaaaatga acatttacat tatgtgatcc caccagaagg atccaatctt
    16621 tggtttgata atatcgttat gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc
    16681 atcaacttta tgctagaacc ggaaaatgcg gcacaaaatg cagagtatat tggttattct
    16741 acaccaaatg aagaagcaaa agcgttattg ccaaaagaca tttcgtcgga cgaacaattc
    16801 tatccatctg atgacacgat ctcccatctt gaagtctaca aagatctagg atcgaaatac
    16861 ctaggcatct ataatgacct attcttagaa ttcaagatgt ataggagata atggcaaaac
    16921 tcagtaagaa tggggactta tcaacgttct aaagagctaa aaatcgtcct ttttctatct
    16981 gagttttcca atcttctacc aaaagcggat gaaacgccgc tttttttgtg ttattttatt
    17041 ttttctacca cgtttttcta ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg
    17101 agacgggcta gtaaatctaa gattgaaaaa gttacagaag gataaagtaa ttggaaacta
    17161 tgcaaatata cttaaccctt ctgtaatcta ttaataaaac taatatatga atccagttca
    17221 atatttgtag ctatgaaaaa acataaaaat catattagat aaaaactaaa ttggtagata
    17281 aattattttg tcagatatct gaggcaaata ttcgactatt tgtgtttcaa agtaaaaatt
    17341 ggaatacaaa tatagaatag acatattgca gccgatagtt tattatgctt ttagacacat
    17401 taaactggag gaaaaaatga aaaagagcgc taaagatcga gtttttgatg agctgatacg
    17461 actaacaggg aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc taggtatctc
    17521 tcgtcagaat gccagccatt atttgacgag attagtggaa gataaaaagg tcgagaaatt
    17581 accgggaaag cctgttttgt ggaagccttt agatgaatat gcggttgtag ataatacgga
    17641 gcaaataaac gaagcctttc attctgtagt tggtcatgac ggaagtctga gagaggtcat
    17701 tcaaaaatgt atagccgctg tcaaatatcc gcctaacgga ctcagtgtat taataaatgg
    17761 agccacgggt gttggaaaaa gttttttagc cacaaaaatt tttgaatacg ctatccatga
    17821 gcaaattatt gaaaaagatg caccatttgc tatcttaaac tgtgccgatt atgcggacaa
    17881 tcccgaactg ctttcggcaa cactgtttgg atacaagaag ggggccttta cgggggctga
    17941 aaaggatacc gatggtttat tggcaacggc caacaacggc tatctttttc tcgatgaggt
    18001 ccatcgccta tcgaaagaaa atcaagaaaa gctctttcta tttattgata ctggaaacta
    18061 tcgtccagtc ggtgaaaatg taaattggca ttctgctaag gttcgtttta ttttcgccac
    18121 gacggaaaaa ggtgaaaatt atttattaga cacctttgat cggcgtattc aaattagtgt
    18181 tatgttgcca acttttgaag atcgccccat tcgagaacga ttagagctga ttcaattatt
    18241 ttttcaaaat gaagcggatg ttttacaaaa agatattatt gtttcaagag aagccttgtc
    18301 tttaatgtta tctcatccct ttagtgggaa tattggtaag ctgaagaata tcattaaaat
    18361 cagttgtgcg gatgtgtata gtcgaacaca agaagaacca ttaacgattg ggaaaaatga
    18421 gatcttgatt cagttaaata tgctggaatc cgaagtagaa agcttgccga ttgctagttt
    18481 attgataaag aaaaatcaaa agataaaaac ggatcgaatc aaaccgtttg attatgattc
    18541 gattttggaa aaaattactc agctgataaa tgaaggtaat ttcaatgaaa atttttcttc
    18601 gataaagaat gaagtacagc atttggtgca tcacattgat aaggattctt ttttatcaat
    18661 gagtagaaaa ttgatgattc agatgtttag aaaggtctgg gtatccgttt taggaaaaac
    18721 gtacggattg acgacgagtg gacctgttgc agaaattatt agcagatcct atgtcatttc
    18781 cccaagctat aagacagact taaccgggat aaatcgtaca ttagctgcaa atctacctcg
    18841 gacgacttac ttatgtaatc agttcttgga gcatttacca ccgctctcag aaaaggacga
    18901 gaactttcta agactgctta ttgtcgtgac tttatctgat tatgtagatg aatccatcga
    18961 gctgaagggc ctgttgcttg ctcacggaga tagtacggct agcagcattc aagcagtagt
    19021 gaatcaatta tgcggcaatt atgtttttga agcgttggat atgccagtag agagtaatgt
    19081 gtcagagatt gtcgaatcga caaggaattt cgtgaaacgg caagtgaata cagatagtct
    19141 agtgctgatc gtagatatgg gctctttaaa tcaaatttac acacagatca agaacgattt
    19201 aaaaggggag ttacttcttc tgaataattt gacgacttcc attgcacttg atattggctt
    19261 gaagatgacg atgaatgcgc catttaaata gattgcggag gatgcagcag acaagtattc
    19321 aatcaatgtt caatactttg aaggcttttc tcaaagtagc aacattatca tttcctgtat
    19381 gtcgggtttg gggatttcgg acaaattgaa agatgttttt cgtcatcatt tatcggatga
    19441 atccatcgaa gttttcacga aggactacag agaactacgt gaattaatcg accaaaatga
    19501 tgaaacttat tttgacaaga caaaattggt catcacgact tcggatcttc cacgttcctt
    19561 ttcaattcct aatattaatg tttatgacat cttagacggt gatggagcag ccaatttgga
    19621 gcatattttg agtcctgaaa tgaagccggc agaatttaac cgcttgattc aggagctagt
    19681 aaaattcttt tcgttggaag gaattgcgga ccgtttgaac tttctgaacc caacgatcgt
    19741 gatgagtgaa gtggaaacag tcatttctaa atatgaaaat tactatcatt tcactttaaa
    19801 tggccgtgta aaactaaatc tctatatgca tacggcattg atgatggaac gtttattctt
    19861 ggctcggaca gaaaaatctg aagaaccgaa ttatgatttg aaaccagatg aagatgaatt
    19921 ttattatgtt tctagaagta tttttcaatc aatggagatg aaatataatt ttaaagtcaa
    19981 tgcttatgag ctttcgctgc tctatgaatt attaggaccg tatattcaaa aataaagtgt
    20041 ctaaaaaagt gtctaatctg ttttttttag gttagacact ttttttaatg tgaattaacg
    20101 tgattaaacc aatgttttta aaagttggca caggaattgc ttatataaaa gtgaacagaa
    20161 agaaaggaat taaccatatg aagaaaattc taatagcgac tcacggtcac ttagcttcag
    20221 gctttcttag cagtattcaa ttactgacgg ggaaggttca agagattacg gtgattaatg
    20281 cttatgtgga tgagtgtgat tttgaacagg agttgaatgt atttgtaaac caggtgaacg
    20341 agcaggatca gatttttgtt tttaccgatt tatttggcgg aagtgtcaat cagaagatta
    20401 ccaaaacctt tttggagaaa gagattgctg taactatttt gtctggcttt aatctgccaa
    20461 ttctgctcga aatagtattg gcaaccaagg aattaacaaa ggaggatatt caggaactgg
    20521 taaataaatg ccaacaggaa ttaaagatga ccattcttga tgaggtacat gtagtaaatg
    20581 aagaggaaga ttttttttag gaggaagaaa aatgattaaa gcagtgcgaa ttgatgaacg
    20641 attaattcat ggacaagtag cgatgacttg gacaaagagt ctaaatttaa ccgggttagt
    20701 agtagcaagc gatgaggcgg ctaccaacga tattcaaaag atgacattga agatggctgc
    20761 accatcgaat gtcaaagtca ttattaaaac agttgatgac gcgattcatt tgttaaaaga
    20821 tccgcgggcg aaggacatgc gcttgatggt tttagtccct aatgtcaaag atgcggtaaa
    20881 agttgcgaaa gctttcccaa cggaaatcga attaatgaac gtggggaatg ctgggaaaat
    20941 gacaccgcca tcggaagata agaaaacctt atcaaaggaa gtgatgttaa cgacgtcaga
    21001 aattcaagac ttaaaagaat tagttgaact ttatccagat accgtttttc agcctacacc
    21061 ggcgatggaa aaacgttcag ctatctctat tttgagtgat ttttagaaaa aggaaggaga
    21121 aaaattatgt atgatgcatt tgtagtcgct ttggccgttt ttattggagt tgctggacat
    21181 gaaatgttcg ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt agcaggagcg
    21241 ttgatggggg atttgcagac ggggttgatt gtcggggcgt cactagaagc catctttatg
    21301 ggtgtcgtca atatcggaat ctctagtacg gcggagcctt ctttggctgc tgggctggct
    21361 acagcgttcg caatcaaaat gggaggaaac gtggatgcga ttattcccat tgtctttcca
    21421 ctagcagtat taggattaca gttaatgaat atgatttttt cttttgtatg tggaccgatg
    21481 gctgctaaat ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat caatctgcac
    21541 ttctttatgt ggtttgtcca ctatgcgctt tatgctctta ttccattctt tgccgtttta
    21601 tttggagcag atgttgtcaa aagcgtcttg gataatattc cagaggtcat catgaacgga
    21661 ttaacagtcg ctggaaatct gctgccggca gttgggatgg cgatgctgtt acggatgctt
    21721 tggagtaaaa atattgctgt gtggtttttc tttggggccg ttgttatggc gtatctcgag
    21781 ttaccattga ttgcaattgc cgcagttgga acgattattg caattgttat agcccaaaga
    21841 gatctacaac taaaaaatat atctgttcat gcagttcaaa gcgatgctgc tgtaaatgca
    21901 acgcttgacc aagaagagga ggatttcttt caatgaaatt agctgatgga atttctaaag
    21961 atgagaaaaa attggttcgg aagatgtttt ggcgctcgat gactatgtat gcttcggtga
    22021 atccggtgac gatgggcggc ggtggtttcg cctattctat gatgcccttc attaatcatt
    22081 tctacaaaag tgaagaagat cgagctgcgg cgctggaacg tcatacacag tatttttcga
    22141 cgaccattcc ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag aaggaaaatt
    22201 ctgaaaaagc tggttttgac gtggaaagta tcaacgcgat caaaacgagt ttgatgggac
    22261 cattggctgg aattggcgat tcattgttct ggggagtttg gcgtgtcgtt accgcgggac
    22321 tagcgatcaa cttagccaat gccggaaata ttttggcacc aattatcttt ttagttatgt
    22381 tcaatatacc taattacttg atgcgatact atggcggcgt tttaggctat tcattaggct
    22441 ctaaatatat tgaaaagctg tatgcgaacg ggttaattga aattctgaca aaagctgcta
    22501 gtatcttggg tctgatcatg gtaggtgcaa tgacgatcag tatggtcaaa ttcaataccg
    22561 ttttgagctt ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc ctcgatagta
    22621 tttttaaagg aattgtaccg ttattggtaa cattgagctg tttcaaatta ctagataaaa
    22681 aagtcggcgt catcaaaatt atttttggaa tggtcgtctt gggaatttta ttgtcattac
    22741 ttggaatcgc atagtaggaa gctgcagcta ctaggctgca gtttttttag caaggaggga
    22801 aaagatgact attaatgaag taatttattc acccaaacac ccatttttta atgctgagtt
    22861 aaacgacgga aatgtgacta cttcaaacga atacagctac tatcggaata ttcctgatgt
    22921 aaagggattg accttcgatg taagtaatca aaagacatta gcaaaccttg atgtttgcgg
    22981 gacgattcaa acgttgacct tttatcgaga aaatttacta actgaggaaa aaccaggtgt
    23041 ttgggtcaat aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg aagtaaacgg
    23101 gattgtctac catttgtcag aatcggatca tcgtattgaa gtcgatgtga ttcaagactg
    23161 tttgcctaga tatattcatc attattcgac gtttaaggtc attgtagtat cctttgctcc
    23221 catactggct aataagcgtt taagtatgct tgttcaacag gtttacattg tgaatgaaac
    23281 acacgaaagc cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga agtattcgga
    23341 tcagcaaaat gtgttgattt ctcaaaaagg aaataaggac acgatcgcac aacaggaagt
    23401 ggcaagcttc gccattgctt ttgtggaccc aaatgctttt gaagaagtaa tgacttttca
    23461 ggaatctgat atcgaaaaat ggctgaagga aacattgaac tattttgctc agctatttgg
    23521 gcagctgtca atgccaaacg atagagttgt ccatctctat cgtcgtgcac tttatcaaag
    23581 cttttctagt ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt ggggcagcta
    23641 tcctgctact aatcgaattt ggaataagga tatgtattac gcatcattgc cattcaccat
    23701 gtttgaaccg gagttatgtc aaaaaacggt tctatggttt gatcaatatg gcgttaagtt
    23761 tcctggttca aaatttacag gaggtatcaa tcattctctg agtaattcgt tatccagtgc
    23821 gttactggct agtctttatt ttgaacatac tggggataca aaattctttg agcggtgtcc
    23881 gcaggttcta ctgaatgctt caagaattat tgaggacata ttagctcaac gtcggtcagg
    23941 ggaaccaatg ctttttccat ctgtttggtt atcagacgcc tttgcacttg gaaagtatca
    24001 taccggcagc aatctttgtc tttggaaggc ctgtattgga cttgcagaaa tttttgaagc
    24061 cctagaacaa ctgagtctta gcaagcgata caaaaacatt gcctgtaaac taaaggaatc
    24121 aatttgtaaa caaatgacta tcgatggtcc ttttggtgag caatttttgg agggaatcgg
    24181 tgatgaggag aaaacaacct attcagtgaa gcactatcaa aaaccgattc ttgagcaagg
    24241 gttaatcttt ttaagtgatg tgattgtgga tggaaaaatt aatttattga tgcatgatgg
    24301 agaagaatca gacactacgt tgatgccttt ttatcaattt ttagataata aagatcattt
    24361 atatcagaac accatggcct tttctgctag ttctttcaat ccaacctata gcgaggaaat
    24421 caaaggcatc acttggggat tagaatccgg cgccactttt cctggcttta tcactgtttt
    24481 aatgagcgag ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac taatttgttt
    24541 ggcagattta gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc aacaaggaga
    24601 agtcgtgcga gactttggct gcggcaaatg cggctgggca tcgggtcttt ttgtcagcct
    24661 aatgatcagt caatatttcg gattgaaatt caaaaaagga gtattgcaaa ttgatcctgt
    24721 tgataattta acgttttctt ggaagaattt gaagttcggt caagccttca tttcgattga
    24781 atgtacgcaa tccgagttga gtatttcaaa tttgtcagaa aagccattga aaataagtga
    24841 tacgaaaaaa attcttcacg tagaaaaggg aaaaacgatt cacattcaaa ggagaaaaag
    24901 atgagtatac taaaaaatat tacccaattt aaaaaaatcc agagtcgagc aatcactccc
    24961 gaaaatccaa caggcgagaa aggtaaagct gcgatgttgc caagcgagct aggaccaagc
    25021 cgcaaaggtc gtggcgcaat tccgctgact tctggtgaag aaactgtgat tgcggatatt
    25081 actggaacgg gaataattcg tcatatgtgg atgacgattc gtgatcgaac agaatttggc
    25141 agttttgttt tacgtgattt aatcttaaga atttattggg atgggtcgga tagccctgct
    25201 gttgaaagtc ctttagggga tttcttttgt aatgggtttg gagagcgttg cgaggtgaac
    25261 tcgttgccga tagtggttaa tcctacaggg ggctttaatt cctatttcga aatgcctttc
    25321 caaacatccg ctaaaattac gatcgagaat ttacatccga aggatatgtc ctcatttttt
    25381 tatacgataa attacgcttt ggtggacgaa ttggaagaaa atagcctata ttttcacgca
    25441 aattggcatc gggaacgcat taccaaatta caggaggatt ataagttaat tgatcgatta
    25501 aagggtcaag gctattacgt gggtacttac cttgcgctta cctgcctaga acgttattgg
    25561 tggggcgaag gcgagtttaa attctacatt gatggggata gtgagtatcc gaccgtaacc
    25621 tcaacaggtt cagaagatta ttttggcggg gcgtgggcct ttcatgagta cgatgagttc
    25681 ggacgtccac atgcgaaaaa ctattcaaca gcctttttag ggtatcctta ccaatcaaat
    25741 agagatgcca caagagatcg ttttgaaacg ggaaaattga atgccgttca tgcctttggt
    25801 gacgacgcaa tgccacgaca tggtctttat cgttggcatc ttcaagaccc catctgtttt
    25861 accgaagatt tatcggtcac tttacaacaa atcggcaacg atgatgttcg cttatttgag
    25921 cggcaagacg atgttgctag tgtcgcttac tggtattcaa cagaagcttc aggaaatact
    25981 aaacgtcatt tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt tcacatattc
    26041 gaagggagga acctatgatg atatatgggg caattgaagc tggagggact aaatttgtct
    26101 gtgcagtcgg tactgaggaa ttaacgattt tggaaagagt cagttttccc acaacaactc
    26161 cagttgaaac aatgaagcag gtcattgatt tttttaaacc ttttaaggaa caattggcag
    26221 gcatcggggt cggctgcttt ggtccaattg atattcaatg caaatcaacg agtttcggtc
    26281 atatcacatc aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga acgctaaagc
    26341 aatatttcaa tattccgatt gaatggacaa cagatgtcaa tgcggcttgc tatggtgaat
    26401 atgttgctgg ttctggcaaa gggttagcag gtgttgcata cttcactgtg ggaacaggta
    26461 tcggaggtgg tgccttgttc aatgggactt tcgtggaagg gttcagtcat ccggaaatgg
    26521 ggcatatgtt gatcaagcga catccaaagg acaatttttc aggaaactgt cccttccacc
    26581 aagattgttt agaggggatg gcagcaggac cagcgattga aaaacggttg ggtgtaaaag
    26641 gccagaattt attggctgac gattcgtttt ggcagattga agccttttac cttgcccaat
    26701 gtgcatacaa tacaacactg atgttttcgc cagatagaat tatttttggc ggtggtgtaa
    26761 tgaaacaaga acacatgaag aaaaaagttc aggataaatt tgttgagctt ataaatggat
    26821 atgttgaaat accaccaatt gatagctaca ttattacgcc cgaattaggt gataatgccg
    26881 gaataatcgg cggattagca ttggccagaa aagcggtaag aaataagcaa ccataattaa
    26941 tgaatgtttc attgatactc atgtcaaaag tatttaaccg agtaatcaaa tgatcaaatg
    27001 aagttggata aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat cgtgttgttt
    27061 tcattaaatc actgaaagga aatgcttctg cgtttccttt tttctttttg ttgtgcgctg
    27121 tataccttga ggatattcac tggtggcgta cacttaaata gtaatatatt gtgttacttt
    27181 atgtctgtgg taatagttat agttgcttac caatgtatgt tcatttttcg aaatgttgac
    27241 ttcataatcc ttggcttaat gtttgaattt ttttggtgat agattttttc ag
//
LOCUS       DAYYCM010000035        27262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000035 DAYYCM010000000
VERSION     DAYYCM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.036"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..1438
                     /locus_tag="ACHAY1_001541"
     CDS             269..1438
                     /locus_tag="ACHAY1_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8593541.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1911..2465
                     /locus_tag="ACHAY1_001542"
     CDS             1911..2465
                     /locus_tag="ACHAY1_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593542.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2638..3075)
                     /locus_tag="ACHAY1_001543"
     CDS             complement(2638..3075)
                     /locus_tag="ACHAY1_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593543.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3123..4070
                     /locus_tag="ACHAY1_001544"
                     /pseudo
     CDS             3123..4070
                     /locus_tag="ACHAY1_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4341..4776
                     /locus_tag="ACHAY1_001545"
                     /pseudo
     CDS             4341..4776
                     /locus_tag="ACHAY1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4792..5625
                     /locus_tag="ACHAY1_001546"
     CDS             4792..5625
                     /locus_tag="ACHAY1_001546"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593544.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5870..6895
                     /locus_tag="ACHAY1_001547"
     CDS             5870..6895
                     /locus_tag="ACHAY1_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593545.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7033..7158
                     /locus_tag="ACHAY1_001548"
     CDS             7033..7158
                     /locus_tag="ACHAY1_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593546.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7183..8298
                     /locus_tag="ACHAY1_001549"
                     /pseudo
     CDS             7183..8298
                     /locus_tag="ACHAY1_001549"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8381..9277
                     /locus_tag="ACHAY1_001550"
                     /pseudo
     CDS             8381..9277
                     /locus_tag="ACHAY1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9293..10117
                     /locus_tag="ACHAY1_001551"
     CDS             9293..10117
                     /locus_tag="ACHAY1_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593547.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10149..11459
                     /locus_tag="ACHAY1_001552"
     CDS             10149..11459
                     /locus_tag="ACHAY1_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8593548.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11607..12866
                     /locus_tag="ACHAY1_001553"
     CDS             11607..12866
                     /locus_tag="ACHAY1_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8593549.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12876..13748
                     /locus_tag="ACHAY1_001554"
     CDS             12876..13748
                     /locus_tag="ACHAY1_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593550.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13760..14590
                     /locus_tag="ACHAY1_001555"
     CDS             13760..14590
                     /locus_tag="ACHAY1_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593551.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14630..16813
                     /locus_tag="ACHAY1_001556"
     CDS             14630..16813
                     /locus_tag="ACHAY1_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8593552.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16874..18094
                     /locus_tag="ACHAY1_001557"
     CDS             16874..18094
                     /locus_tag="ACHAY1_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8593553.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18091..19551
                     /gene="gtfA"
                     /locus_tag="ACHAY1_001558"
     CDS             18091..19551
                     /gene="gtfA"
                     /locus_tag="ACHAY1_001558"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8593554.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19628..20734
                     /locus_tag="ACHAY1_001559"
                     /pseudo
     CDS             19628..20734
                     /locus_tag="ACHAY1_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21056..21265
                     /locus_tag="ACHAY1_001560"
     CDS             21056..21265
                     /locus_tag="ACHAY1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8593555.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21258..21407
                     /locus_tag="ACHAY1_001561"
     CDS             21258..21407
                     /locus_tag="ACHAY1_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8593556.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21561..22226
                     /locus_tag="ACHAY1_001562"
     CDS             21561..22226
                     /locus_tag="ACHAY1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8593557.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22510..23595)
                     /gene="xerS"
                     /locus_tag="ACHAY1_001563"
     CDS             complement(22510..23595)
                     /gene="xerS"
                     /locus_tag="ACHAY1_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8593558.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23929..24162
                     /locus_tag="ACHAY1_001564"
     CDS             23929..24162
                     /locus_tag="ACHAY1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8593559.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24159..24566
                     /locus_tag="ACHAY1_001565"
     CDS             24159..24566
                     /locus_tag="ACHAY1_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8593560.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25703..26221)
                     /locus_tag="ACHAY1_001566"
     CDS             complement(25703..26221)
                     /locus_tag="ACHAY1_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8593561.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26588..26836
                     /locus_tag="ACHAY1_001567"
     CDS             26588..26836
                     /locus_tag="ACHAY1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593562.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9326 a         4140 c         5034 g         8762 t
ORIGIN      
        1 gtagtataat ttaaaggtaa taagttattc ttttattact atgtttgggt tttatagcaa
       61 agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat taaatcttat
      121 tatctttagt gatataacga aatatctaca cagacaaaat gatagcataa aatattttgt
      181 gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac
      241 aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac
      301 actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa
      361 ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg
      421 gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg
      481 agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc
      541 tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg
      601 aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc
      661 acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag
      721 gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag
      781 acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa
      841 agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct
      901 tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag
      961 tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt
     1021 ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag
     1081 cgatttcaaa ttagtttatc aagcaagttc aaaagaagaa gcaaataatc aaataaggtt
     1141 tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca tgaatcctgc
     1201 tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt caactaattt
     1261 gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc aatttcctaa
     1321 tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc aaaagtttct
     1381 atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa tgttttaact
     1441 tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctcccg acaaaattat
     1501 gatcttgcgt aaagttataa aatattaata aaaatatttt attaatgaaa agcttggtag
     1561 cacggttatt catatgctaa tttatttcac tttatagtaa ggggagttct tagtatattg
     1621 ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag aacttctacg
     1681 gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag taaggttaat
     1741 ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa atcataaact
     1801 aaatcccttg attttattga ctttcaaaag tttatatata taagttaatg attatggtaa
     1861 aatatataca taatcattaa ctcagaatat ttttaagaag ggaaacaata gtgagctata
     1921 atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa
     1981 caagtaatcc ggaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta
     2041 tgtcagatat tataggatta aaagttggag atatacgagg caaaagtaag cctattattg
     2101 ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga gaagaaattt
     2161 tattatatac tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg caacaaggtc
     2221 gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggac
     2281 gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa
     2341 ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa
     2401 ggtacattgg tataactgaa gatgaaatag gagcatcact tcgtggattt aaactaggtg
     2461 tataatttaa aaatttttat aaacagtaaa gacaaagatt aaaagctatt tttatctaga
     2521 acgattttat ttcataaagt tcgcggaata aaagctgggg ttactggtac ttggtatagg
     2581 ttcgtgttat ataaactaag taattaaata acatttttag gttggaaaag gatgaaatta
     2641 aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg cggaataaga aaaatactga
     2701 taactcaacg tttagcagac tatctaatag ggatagtctg cttttttcgt ttttgaccat
     2761 acttttgatc ttctgcaaac ttccttgctt ttcctctgta gaactcatga aatctgcaaa
     2821 acgaattgcc gtttcctctg tctgcttttt cgcgatatgg gtgtatatat catccatagt
     2881 ggttttaata tctgtatgtc caagtcgaac atacacttct ttgacagacg caccagattc
     2941 aaagagcaga ctacaatggg tatgacgaaa accatgaagt gtgatatgtt ttaagttatt
     3001 ctctttaatg atgattttca aattgtgatt tacatagtct aaataaaggg gcttattttc
     3061 ttctgatgaa aacaaaagtt gttcaccatc ttttataggt ttgattctca tttttagata
     3121 ttatgcccgc acgtattcat cacatttcta ttgtgaatcg ctttattcga ccgacatttg
     3181 acttttatca taatatactc ggtttaaaat tactaatgaa aacaattaat caagacgatc
     3241 ataccatgta tcacctgttt ttttcagata atcaccaccg tgtgggaact gaactgactt
     3301 tttttgaagt acaagaaggg aacaaccagt tttttggaac caatacgatt gaacgaacga
     3361 tactaaaagt tccttccgaa gcatcattgc atttttggga aatatatttt gaaacacaag
     3421 gcgtttgcca ttatggaatc gaatcatttt ctggacgacc tatcttacgt tttgaagggc
     3481 cagatgcaac acgattagct ttagttccct tacgcgaatt tgaagatatt gatgattatt
     3541 tcccgtatgt cacagaccag atccctacag agcatgccat tttaggaatt gatgccattc
     3601 aactacgcgt acaatattcc gatgcgactg aacgagagtt attgtcggta ggctggtagc
     3661 acaaagaata cgtcagtttc ttttcgggtc atcaacgagt gacaatctta gaaaatcaag
     3721 acactcagtt ctatcaagaa gttcagatta ttcaagatcg tgagaatcca gttgccattg
     3781 aagggattgg tagcgtccat catgttgcgt ttggcgtaga aaataaatct gatttacaac
     3841 gcatcgacaa acaattacaa gagcgcaatt ttataaattc aggaataaaa gatcgtgaat
     3901 tttttgtatc actttattat cgtgacccaa atcaattatt gattgaaatt gctacagggg
     3961 aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa gaaattcctt
     4021 tatttttacc gcagtactta aatttcattc gtgaacaagt ggagcaataa ttagctaatc
     4081 aaatccatga cgaagagtaa gaggttgact cataagtcaa aaaataaaga aaaacgcatc
     4141 aaatccattc taaagaatgt tgatttgatg cggttcctat ttttataaac tatcattttc
     4201 gactaataaa actacttatg agtcagcctc tctttttttc ttgattttta tacctctttc
     4261 taggtaaact aaacatctgc caaaaatacc gtgtctcatt gaaacattct caactatttc
     4321 aaaataaagg ggagtaccaa atgaacgagt atattgaaat tatccacgca agagaaacaa
     4381 cttaaaaatg tcagtctcaa aattcccaaa aataaaatta gtatttttac cagcgtatct
     4441 ggttcaggaa aatcatcgat tgtttttgat actattaccc aagaagctgg tcgtcaactg
     4501 aatgacacct atgatagttt tacccgttta tttttaccta aatacagtcg tccagaggtt
     4561 gatgagattg ataatctttc tacggcgatt gtggtcaatc aaaagaagat tggtggcagt
     4621 gcgcgctcaa cgttagggac gattactaac atcaatgcgt tatttcgtgt gttgttttca
     4681 tgttttgcaa cacctagttt aggatatgca aatgcttatt catttaatga tcctagtgga
     4741 atgtgttctg tctgtcaggg aatcggtaag acgattgcat ttttaatagg attgctttat
     4801 ttttcggcta ttgataatgc tgattttgta ttttctgatc catttaccgc agaagttttg
     4861 tctggaattg aggaacaggc aaagaatcat ggttattttg tattggtgca taatgttaat
     4921 tcagcagaag atgttaaaac tattcagaga aattggcgtt ttgaggggtt cattgttgtt
     4981 ggtgtacgtg taaaagaatt tttggaatta aataacgttt ttgatggtcc tgttgtttat
     5041 attgatacgc atctttcaaa agagacatta agagaacttg aacaacaaat gaatcgttta
     5101 tttgtaaata caaatgatta ccaagctagt acattggcag tggaacattt gattcattat
     5161 ggtcatgaga aaatagcgtt tctttctttt gactacattg taggtgaacc aagtgttatt
     5221 caagaacgac atcaagctta tatcgataca ttaaagaaat atcatttaac ctttaaatcg
     5281 cattggatat ataacaactc tgaatttgag cgaatttatc aagaattagc tgaatttact
     5341 gcaattgttg ttactggaga ttatttagcg gctaaattag taaaattttt aaaaaataag
     5401 aataaacgag tgatggaaca attatcgatt attggatttg atgatattac ttttgcagaa
     5461 ttaatggatc ctgcgttgac aacaatacga ctaaatccag gtaacaaggg ggcgattgct
     5521 tttcaccaac tgattggact ggccaaacaa gaagaaataa aaaatcaagt actactatta
     5581 gagggagaat tgatttctcg agactctgtt cacaaaatca aataaaaaag ctctgtgaaa
     5641 taagtatagt taacaataaa atatccgaac gatagagaga atgaactgtg atctagcagt
     5701 tcctcgtcgc aaattcacta gaattagcat tatttactct aaattgttcg gatatttttt
     5761 cttgttttta attatgatta cagtgcctat ttttaacgaa atttgcttat tttcaaaatg
     5821 tatgattata tgttagtaaa cactttcaaa tatgaaaaga ggagctatta tggtaggaat
     5881 tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga
     5941 ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag attgcagaat cggttaattt
     6001 ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg cattaataac
     6061 gacagtttct gaagtagatg agttggaaga tccctatttt cgaacgattc gcagagggat
     6121 tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac
     6181 accgttggag caagacgatt tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct
     6241 atcttctgtg attgaagaag tatcagcaat taattcgaat gtcgtagtga ttgatgaccc
     6301 tagtattgaa gctgaagtag atgcagttta tactgattta acaaaagcaa cgaatcagca
     6361 tttagatcga ttgtataaaa aaggacaccg aaacattgcg tttatcggag gaggacgtgt
     6421 tcgtttagat agccaaggaa ataaacacgt gtcagatgat gattatcgaa aaatagccta
     6481 tgagacgtgg atgaaacaaa aaggattaga aaaatatagt caaatttatt tagaaggctg
     6541 gaccacactt gacggaatgc aggcagcaga tttacttttg aaaaaacatg ctaaagataa
     6601 gttgccgact gctatcatgg taggaaatga tcctattgct gtgggggttt atcgttcact
     6661 tcaaaaaaat ggtctacata ttccagaaga tatttcgatt gtgagttttg ataatattga
     6721 agttgctgaa tttttaacac ctgcattaaa tacggtgaat gtgaatacag aagaaattgg
     6781 aaaactagca gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa
     6841 agtgattgtt tctaataata ttattgttag agaaagtgaa aaaaatatcg attaaaaaag
     6901 taaacatcca aaaaagggtg tttacttttt tgtttactaa tatttttagt aaaaaatctc
     6961 gacaaaacat taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca
     7021 ggaggaatta agatgaaaca tttcaaaaaa atagctttaa cagggatagc tgttttaact
     7081 gggacattac tcgcagcatg tggtggggga gatagtaatt ctaaagacgg gcaagtaact
     7141 ttaagctatg cgagttgaga tactgaccaa gctccaggac taagaaaaat gttagatgaa
     7201 tttgaaaaac aaaatcctga tatcaaagta gaaatggaga caacgccttg ggatcaatat
     7261 tgggttaaat tagaagctgc aacaacggga ggtaatatgc cagatgtagt aaccatgcac
     7321 tctagcgaat cctataaata tatgtcacag aatgctttga tgggacttga tgatgttatc
     7381 aaagaaaatg atattgacat gggaaacttt actgaaggaa ttgctgattt ctatacgctt
     7441 gaagacgaac tatacgcaat ccctaaagat gcttctgtag tggggctatg gtacaacaaa
     7501 gaattatttg ataatgcagg tgtcgattat ccagatgaaa catggacatg ggaaacactc
     7561 agacaagctg caattgattt aacagatgaa tcaaaaggca tctatggttt tgcatcacat
     7621 attggaacag agactggcta ctggccattt atttatcaaa atgggggaga agtatttgcc
     7681 gatgacaata caaaatcagg tttgaatacc ccagaagcac aagaagcttt agctttttat
     7741 cgagatattg cacttgttga tcatgcttca ccaacaatta cagaattaca agatcctgat
     7801 aagttaagtc gttttcaatc aggtagatta gcaatgatga ttgaaggaaa ctggtttgca
     7861 ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt gttacctaag
     7921 ggaaaagtta atgcaacaat tacaaatgga cttggttggg ctgccagtgc gagtacaaaa
     7981 tacccagaag aagtgaaaaa actaatggca tttttagcta gtgaggacgc caataaaatc
     8041 caagcggaaa cgggggcatc aatcccagca ctaaaaggct ttgaagacga gtgggctgct
     8101 tcttcagata caattgattt gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt
     8161 ccatacaata aagatggatt acgagctgaa caagcagaag ttcaaattat taaccgtatt
     8221 ctgtctggtg aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg
     8281 atattgagcg gaaattaaat tagttaatta tacaatatac aggcgaaagg attctatttc
     8341 gtctgtatat taatggatga ataccaaaaa gggggatatt atgaaaaatc gaatttttgg
     8401 aaaatatcgt aaagatcaag tgatatgggg ctatattttt atttttccaa cggcattagg
     8461 attaatttta ttaaatatat ggccagcaat tcaaacagca attttaagtt ttcaaaaaac
     8521 ggtagggttc ggtgatactg aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga
     8581 cggtgaagtt tttcagtctt tattaaacac attaatttat gcagtggttt cgatacctag
     8641 tattgttgta ttatccttgt tggtggcagt tttaatgaat agaaaagtta aaggattgtc
     8701 gatttatcgg acaatttatt ttctaccagt cgttgctgca ccatccgcag tcgcaatgat
     8761 ttggcgctgg atgttcaata atgattatgg actgattaat aacatgttgg ctaaaatagg
     8821 tctcgatgga ccagcatggt taacggatcc atcgattgcg atttattcta ttatcattgt
     8881 aggagtttgg agtgctattg gttataacat ggtgttatta ctagccggct tataagaaat
     8941 tcctaaagat tattatgaag cagcggaaat tgatggtgct agtccaatcc agcagttttt
     9001 caacattacg ttaccattag ttacaccaac attatttttt gtagttgtaa caacagttat
     9061 caatgctttc caagtatttg atgttatttt tatgatgatt actcctaata gtaccgccat
     9121 gtttaaatca caatcattag cctatttatt ctataaacat tcatttattt taaatgacaa
     9181 gggatatggt tcggcaatcg ttatgttcct tttagtgatt atattaatta ttactgcaat
     9241 tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa
     9301 taaatttttt tcaaatgcgc caatccatat catgcttatt ttaggtggta ttgcaatggc
     9361 attacctttt atttggatga ttttaacttc agggaaaacg ttggttgaat ctacccaaat
     9421 tccacctaca attttccctg agaaatttca gctaagtaac tatcaacaag tacttgattt
     9481 attaccattc atggcttttt atatcaatac atttttaatg gttgttggac gagtgattgg
     9541 ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa
     9601 aaatatcctt ttcagcttag tacttattca aatgatgatt cccggttcca tttttattac
     9661 tccacagtat ttattagcac aaaaattggg tattttaaat acaattccag cattaatatt
     9721 accaggggta gttagtgcgt ttggtacgtt tttattaaga caatttttct taggtttacc
     9781 tgatgaattg gaggaagcgg cctatttaga aggtgccaat atttggacga tctttttcaa
     9841 aattatgttg ccgcttgcta aatcaggatt ggttgcactt ggaatattca cagcgttgtt
     9901 tgcatttaaa gatttaatgt ggccattgat tgtcaatact tcaattgata agatgccttt
     9961 agctccggga ttagcaaact tacaaggaca atatgccacg aacttcccac aattaatggc
    10021 aggttcgtta ttagcaattt ggccaatagt tgtgatattc atcgttttcc aaagaaaatt
    10081 cattgaagga attgctacaa caggtagtaa attataaaac tattaattag tgattggaga
    10141 gtaagtacat gacaaaaatt acatttattg gtgcaggaag tactattttt gcaaaaaacg
    10201 tattaggcga tgcgatgtta actccaagtc tacaagatgc acatattgca ttgtacgata
    10261 ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa aaatattaat aaaaattcga
    10321 atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa agaagcgtta agagaggctg
    10381 attttgttat caatgctatt caagtgggtg gttataaacc ttcaacagtg attgattttg
    10441 atattcctaa aaaatatggg ttgcgacaaa ctattggtga caccatgggg attggtggga
    10501 tttttagaac cctacggaca ctaccagtaa tgttagactt tgctaaagaa atggaagaag
    10561 tttgtccaaa tgcttggtta ttgaactata caaatccgat ggcgatgtta accatgggtg
    10621 ttttaaaagc gacgagtatt aagactgttg gtttatgtca tagcgttcaa gtatgcgtac
    10681 cagaattgtt tgagcattta ggaatagccg atcaatatga cttaaatgac tttcaatgga
    10741 aaattgcagg cattaatcat atggcattct tgttggaaat tacaaaaaat ggggaagatt
    10801 tctatccaac aattcgtcag ttagctgcag aaaaaccaaa tccacatcca gattctgtac
    10861 gttttgaatt gttgaaacgt tttggttact atattacaga atcaagtgaa cacaatgcag
    10921 aataccatcc atactttatt aaatcaaaat acccagaatt gattgacgaa ttagggattc
    10981 cgttggatga atacttacgt cgttgtgaac aacaaattaa tgattgggaa agcatgcgag
    11041 aggacattgt aaataatggg gagttaacac atacgagaag ccgtgaatat gcttcatata
    11101 tcatggattc tattacgaca ggaactccgt cagtgattgc aggaaatgtc ttaaataaag
    11161 gcttaatcac aaacttaccg acagatgttt gtgtggaagt accttgttta gtcgataaaa
    11221 atggcgtgca accaacctat gtcggtgaat tgccaacaca attagctgct ttaaatcgga
    11281 cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt aacactagat aaatcaaaaa
    11341 tttatcaagc agcgtattta gatccacatc tatcatctga attgtcttta acagatatca
    11401 aaaatttagt ggatgattta atcgaagcac atggggacta tttacctgaa tataattaaa
    11461 aagaagctga aagagtaagg aattttcaat tccttactct tttcttagtt aaatttagta
    11521 aaaatattga caaaacactt agtaaacagt tacattagtg ttaacaaaaa gaaaacgcat
    11581 tcaaattgta taaggaggag cataaaatga agtggtctaa aaaaattatt ttagcttgta
    11641 tagcactagg agcaacgatc tctttgactg cttgtggaaa aaatcaggaa gatgcaagcg
    11701 gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat gactcaaacg attcaggaga
    11761 ttgctaaaga ttttgaagca aaaaaccctg atattcatgt aaaagttgtg gatgtaccga
    11821 atgctggtga agtaattaaa acacgtatgc ttgcaggcga tgtaccagat gtcattaatc
    11881 tttatcctca aagtatagaa ttgaaagaat gggcgaaagc tggttattta gaggacttaa
    11941 ccgaagaacc gtatttagag aatatcaaaa atggatatgc acaacgtttt gctattgagg
    12001 atcgtgtata tagcatacca ttaaccgcta atgtctatgg tttttactat aacaaaactg
    12061 cttttgagga aatggggatt caagcaccag aaacttgggc agaatttgaa aaaatagtag
    12121 ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa ggctggactt
    12181 taaatggtta tcaccaacta gctatagcga ctgctgctgg tggtgaagag gaagcgaaca
    12241 atgtttggcg tttctcagaa gttaatggaa ttaatgctga aagcaaagaa atgcaaaaag
    12301 attttaatcg attagatttg ttaagagaac caaaagcttt gcaaaacaac tggcaaggtg
    12361 ccggttataa cgatacagta gttactttta caaaaggtga tgcgttgatt atgccgaatg
    12421 gttcatgggc aatgccgatg attcattcac aaaatccaga ttttgaagta ggaacttttc
    12481 cttttccagc agatgaagca ggtcaaagtc taacgattgg cgcaggagac ctagccttat
    12541 cagtttctgc aacttctcaa cataaagaag aagccaaacg ttttgtagaa tatatgacaa
    12601 cacctgaagc gatgcagaaa tattatgatg tagatggtgc accgtgtgct gttgagggcg
    12661 ttatcgagaa tactcaggat tcaccattaa gtggtttaac tgaacttgct tttactgatc
    12721 gtcatttagt ttggttagcc aaagattgga atagtgaaaa tgatttttat accttaacaa
    12781 caaattattt acacaatggc aatcaaaaag ccatgattaa cgcattaaat gctttcttta
    12841 atccaatgaa agctgacgga aggtaggaaa ataaaatgaa aataaaagaa attttaaatc
    12901 gttactgggc aatcattttt gtagcaattc caattatttt gcagatgatt tttttctact
    12961 ttccaatggt tcaaggtgct ttttacagtt taactaattg gactggtctg acttataatt
    13021 ttgattttgt gggtttgaac aattataaga ttcttttttc tgatccgaag tttatcaaat
    13081 cgattggctt cactttaatt gtgacgcttt gcatgattgc tggacaaatt ggttttggta
    13141 ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa aacattcttt agagcatggt
    13201 tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa caaattttta
    13261 actatggttt accaaaaatt ggtagtatgt taggcattga atttttacaa accagtcttt
    13321 taggaacaac atggggagcg atttttgcag taatttttgt tttactttgg caaggaattg
    13381 ccatgccagt catcattttc ttagcgggtt ttcaaagtat tccacaagaa attattgaag
    13441 ctgctagaat ggatggggcg aatgataagc aaattttttg gaatattgaa atcacctact
    13501 tattaccaag tatttcaatg gtatttatta tggcattaaa aggtggctta acagcatttg
    13561 atcaaatttt tgctttaact ggtggtggac caagcgatgc cacaacatct ttggggttac
    13621 ttgtttataa ttatgctttt aaaaacaatc agtttggtta tgcgaatgct attgctttag
    13681 tcttatttat cttaattggg attgtttcac tggcacaatt aaaattatcg aaaaaatttg
    13741 aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta aatacttgct
    13801 tttattatta gggggcttca tgattttagt tcccttagtt tcaacgctat tcggttcatt
    13861 taaaacaaca cccgatatta tgaataattt ctttcgtttt cctaatccag cgactttgga
    13921 aaatttccga agattgttag cggatggaat tggtcattac ttttggaatt cggcaatcat
    13981 cacaatcatt gcagtagttg ctgtgacagc aattattcct atggctgctt attcactagc
    14041 aagaaaaatg actcgtggga aagcatacgg attgatgtat ttatttttaa ttttaggaat
    14101 ttttgttcct ttccaagtaa ttatgattcc gattaccgtt atgatgagta aaattggttt
    14161 agcaaatatg tggggattaa tcttactcta tctcacttat gccgtaccac agacactatt
    14221 tttatatgtt ggttttatta aactaaatgt gcctatcagt ctggatgagg ctgctgaaat
    14281 tgatggggct agtcacttta caacttattt caaaattgtt ttcccaatgt taaaaccaat
    14341 gcatgcgacg actattattc ttaatgctct atggttttgg aatgatttca tgctaccatt
    14401 attaatttta aatcgtgatt ctaatatgtg gacactacca ttgtttcaat ataattatac
    14461 gggacaatat tttaacgact atggtccaag ttttgcttcc tatacagtag gaattgtcac
    14521 gattacacta gtttatcttg ctttccaaag aagtattata gctggaatga gcaatggagc
    14581 agtgaaataa ccaataaatt attctcatta taaaggagtt cataaactta tgattatatt
    14641 tgacaatcac acacaaacat ttcatttaca aaataaaaaa attagctatc tattaggaat
    14701 cgaagaaaat aattatttaa cgcatcttta ttttggaaaa aagattacgc actattctgg
    14761 tgggtatcgc tacccccgaa cagatcgttc cttctcaccc aacaatgcaa atgcgcaaga
    14821 tcgtttatta tctttagaca cattaccatt agaatttcca ggatatggac atggtgattt
    14881 tagagaaccg gcgcataata ttaaattagg aaacggtagt cgtgtaaatg attttcgtta
    14941 tgatagctac gaaattcaaa aaggaaagcc cagcttagcg ggattgccag ccttatatac
    15001 ggaagatgat gatgaagctg aaacgctgat tattcaattg aaagacgcga ttagtggatt
    15061 gactttacag ttgttctaca caatttatca aaatcacagt gtaattattc gttctacgca
    15121 attagtaaat aatagtgatg aaacagtgga gattaaccgc ttagctagtc aatcaattga
    15181 ttttccaaat cgtccactcg agttaattca tttaaatggt acctgggcaa gagaacgtca
    15241 aatgactaga gaaaaaattc atacagggat aaaagtatta gatagtaaac gcggatcaag
    15301 cagccatcaa caaaatccgt ttgttgcttt agtagatgcc aaaacagatg aatttcaagg
    15361 agaggcatat ggatttaatt tagtttatag cggtaatcac gaaacggtga ttcaaaaaga
    15421 tccatttgac caaacacggg tcattatggg aattaattca tttaattttg gttggcaact
    15481 aaaaactggc gactcatttc aaacgccaga agtcgtgatg gtttatagta atgaaggtct
    15541 gaatacgtta tcacagacgt atcatgaatt gtataatcac cgtttaattc gtggaaacta
    15601 tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa gcaacgtatt ttgattttaa
    15661 cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac ttgggcattg aaatgtttgt
    15721 tttagatgat ggttggtttg gcggtcgtga aaatgacttc acttcattag gtgattgggt
    15781 tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta gccaagcaag tgcatgataa
    15841 cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt tctgaagata gtgaactatt
    15901 tcgagcacat ccagattggg cattgggtat tccaggtcgt gaccgctcaa ttagtcgaaa
    15961 tcaatatgtt cttgattttt caagacaaga tgttcgtgat aatatctatc aacaaatgac
    16021 ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc gaaatatgac
    16081 agaagtctat tctgtcttgt tggactcaac gatgcaaggt gaagtggctc atcgttatat
    16141 gttagggtta tatgaatttt tagaaaagtt aacaacgaat tatccaacta ttttatttga
    16201 aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca tgccacaaac
    16261 ttggacgagc gacaataccg atgccgtggc tcgtctgaaa attcaatatg gaaccagctt
    16321 agtgtatcca atttcaagta tgggtgcgca tgtttcagca gtcccaaatc atcaaacagg
    16381 aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt ttggttatga
    16441 gctagatcta acgcaattaa cttctgaaga aaaagcagaa attaaagaac aagttgcctt
    16501 ttataaaaag catcgccaac ttttgcaata tggaactttt tatcgcttgg aaagtccgtt
    16561 tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac caaacagaag ccatggtctt
    16621 ttatttccga gtattagcag aagctgcgca tccattagtc acattgaaat tagcaggatt
    16681 agcagaagaa gcaacttatc aaatggaaga tgtaacattt gctggcgatg aattgatgaa
    16741 tctcggtttt tatattgact cagaactaca tggtgattat gcgacacaac gcttttattt
    16801 gaaacagata tagtagcttg aatttacaat ataagtgcaa caccaaaaga gactcattcc
    16861 aaacgaagaa aatatgtttt atttacacgc atttactaaa aagcagcctg atttgaattg
    16921 ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt aactattggt tagataaagg
    16981 actaggcgga tttagaatcg atgcgatatt aaacatcaaa aaaaaactag agtatggcat
    17041 ctttgaatct gatggagaag atgggttagc cttcattggg ccctggattc ttaatcagtc
    17101 gggcattgaa gtgtggttaa aagaaatgag agagcggaca tttaaaccac ataatagtat
    17161 gacggtagca gaagccgatg ttcctaatga acgtttagat gaatatattg gtgaagatgg
    17221 ctattattcg atggtttttg attttagtta tacggatatt gatgtacctg aaactggtga
    17281 atggtttaaa gatagccaat ggacatggac ggatatgcgt accaatattt ttacgaatca
    17341 attagttacg caagataaag gttggggcgc cttatattta gaaaaccacg atcaaccacg
    17401 ttcaattaat aaatatattc cagaagaatg gatcaatgat tatagtaaaa aaatgttggc
    17461 gacgcttttt atgttactaa gaggtacccc ttttatttat caaggacaag aactaggaat
    17521 gaccaacata gaaatggcat cattagaaga ttttgatgat gtagctacgc attctcagta
    17581 taaacgggca ttagaatatg gattggcacc agagcaagca ttgaaagctg tcaataaacg
    17641 aagtcgggat aattctcgca cacctatgca atggaatacg caaaaaaatg ccagattctc
    17701 aacttctgat aatgtatggt taaaagtaaa tcctaattat ccagaactca atgcagaagc
    17761 tcagttaacc aatcaacagt cggtattcaa ttattatcat caattaatta aattaagaaa
    17821 aaatagtgta tacaaagacg tgctaatcta cggacaattt ctaccagtga aagataaaaa
    17881 tgaacatatt ttgctttatg aacgccaatt agaagataaa atcgtattag ttatcttaaa
    17941 cgttacagca acagaacaag aatattatgt agatgaacag taccaacaag tattaatcaa
    18001 taattatgaa gatgttttat tagatcaaag tcgaaaattg cgtttacgac cgtttgaaag
    18061 tattgtatta gctaattatg gaggacaatt atgaaaatta aaaacgaagc aatgctcatc
    18121 acctatgcag atagtttagg aaaaaacatg ggcgaattaa aacaagtctt agacaaacat
    18181 ttacaaggtg ttattggtgg tgtacatctg ttgccttttt ttccatcgac aggtgatcga
    18241 ggctttgcgc cgagtgatta tacgacagtt gatccagctc ttggaacatg ggaagatgtg
    18301 gaagcattag gtgaagatta ttatttgatg tttgatttta tgattaatca catttctcgt
    18361 gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc agtcaccata caaagaaatg
    18421 tttatccgta ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga tattgattta
    18481 atctacaagc gtaaagacaa agcgccgttt caaacggtag aatttgcgga tggtacgaca
    18541 gaagaggttt ggaatacctt tggggaagaa caaattgatt tagatgtgac caaagaagtt
    18601 gtgaaagaat ttattcgaga aaccatcaaa gatatggcga accatggctg ttctttaatt
    18661 cgattagatg cgtttgctta tgcgattaaa aaattagata ccaatgattt cttcgtggaa
    18721 ccagatattt gggaactttt agacgaagtg cgtcaagaag cagcgaaata tgacgtcgaa
    18781 ttattaccag aaatccatga gcattattcc attcaaatga aaattgccaa tcatgattat
    18841 tatatctatg acttcgcctt gccaatggtc actctgtatt cactttatag tggaaaatca
    18901 gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc agtttacaac attggatacg
    18961 catgatggaa ttggtgttgt ggacgctcgt gatttattaa cagatgaaga attggattac
    19021 acctctgagg aattgtataa agtcggggcg aatgtgaaga aggtctattc aagtgccaat
    19081 tacaataatt tagatattta tcaaattaac agcacctatt atagtgcatt aggcgataat
    19141 gaccgtagct atctgttagc acgtgcgatt caagtatttg cgccagggat tccacaaatt
    19201 tattacgttg ggttattagc aggaaaaaat gatattgaac tgttggaagc aacgaaagaa
    19261 gggcgtaata ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga agtgcaacgg
    19321 ccagtgattc aacaattatt tgcactttta aaatttagaa atgaatctgc ggcgtttgat
    19381 ttggatggaa cgattgaagt agcaacgcca tcagaatcag aaatagtgat tacacgtaaa
    19441 aataaaaacg gtagtgatag tgcaacatta acggctaact tggatacgaa agaatttatc
    19501 attgagtcta aagatataaa aattttcact agtgttgcat taaaatttta acctattcca
    19561 tagaacaaaa aatcctttat actagcactt ggatgactta tccaagacca ataaaaagga
    19621 ctcatacatg gataagtata aaacaaatac agcatttaat aaatggtttt ctgccataaa
    19681 attaggaaaa ctttctgtca actcccaaaa atgtattttt gattacaatt cgtatcgtaa
    19741 aaaactgtct tttgaagctg tgctcaagct ctttctctac gcagttaatg aagaaaaaga
    19801 aagtttgcgt gatttgagta cagctcttat cgaccgatcg ctccaactag aaaccgatgt
    19861 gtcatcgatt agtcataccc aattgtctcg agcatttaat gcacttgaac ctaaagtgtt
    19921 agaagaaatt tttcaacaac tgttagaaaa agttcaacat caaatcaaac caaccaagcg
    19981 aaatcatctg tatctgattg attccagtac gttttcgctt agtttaaaac gtcataagtg
    20041 ggcgactttc cgtcaaacaa aatcaggtgt caagttacac ttaaactatt gttatatgga
    20101 cgatactacg atgtatccaa ctgattttac cttaacaaat gcccatgaac atgatctcaa
    20161 tcaactacca gtattggtga atcaaccaga ggcaacttac gtgtttgatc gtgggtatta
    20221 ggactttgaa aagatggatc agatgcattg ggatggctat ttcttcgtca ctcggattaa
    20281 aaaaaacacc aaagttcacg tgatagatac tctggaaaca tcgccagaaa cagaaattct
    20341 acgagacgag ctggttcgtt taggatctaa aacctatcta accgccaatt tccgactagt
    20401 cactgttcaa gataagaacg gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc
    20461 ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca
    20521 catcaaacaa cacatgacca ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa
    20581 tcaattgatt ttaacgatga tttccgctct cctcacgttt ctcatcaaat tggagacgaa
    20641 aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt tatttactgt ttcagccctt
    20701 tgaatgttgt ctagaaaaat taattcccac atgagaaaaa agaaatttgg ctcgttactt
    20761 ttggttttca gttgaataac agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag
    20821 cacagttttt tttgacttat tttcagagga aatagttttc agaatattct aaaatgtttt
    20881 atgcaacact agtgatattt tttagaatta gatttctaat gtgcattttt tgagtattaa
    20941 ggagcaagta tacaaagatt tataaagctg attaaatttg aataaaaata tgcaggaacg
    21001 ctactatttt tagtagtgtt cttttttata tttgaataaa aaatgtggtg gttaaatggt
    21061 ggttataaca aaaaatgaat taatgaaatt aggttatggg aagtatcagt ctgagagaat
    21121 catacgacag gcaaaagcaa ttatggcttc aaaaagctat acctattacg aaaatagaag
    21181 attaggacta gtacctgttg aatctgtaga agaagtttta ggtatgaaga tagaggtaga
    21241 ggaggatttt cagaatcatg gttaatttta aaaaagataa aaatgggaaa tatcatttta
    21301 ctgctagttt gggattcgac aaacgaacag ggaaaagagt acaaaaaaac gtagaggggt
    21361 taaaacagtt aaagaagaaa aaaagcgtat accgaacttg tttctaatta tggaaaagaa
    21421 gcagaaactt ctcaaagtac tatgttgttt gaggagtttt tttataaatt attcctacct
    21481 tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac aatcaaaaaa
    21541 acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac attcaaaagt
    21601 ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg tatgggattt
    21661 ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat cctgcaaaag
    21721 ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa gaagaattta
    21781 caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt tttatttgta
    21841 tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta acttggaaag
    21901 atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat aagagagcca
    21961 aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc gaactagacg
    22021 acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata ggaggcatgt
    22081 cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt catattatga
    22141 gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta aggcatagtc
    22201 atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa aggttaaaac
    22261 atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat tcattgagag
    22321 agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc aaaccgacac
    22381 ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca aaagaaacac
    22441 actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc gcctatttat
    22501 acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta tatgggtata
    22561 taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata ccaatgaatt
    22621 tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc tgacttgaat
    22681 tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg atatacgctt
    22741 ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta ctttatatcg
    22801 ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg ctatattgac
    22861 agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta aagataggtc
    22921 atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa tcagtgcatt
    22981 gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt tactcaaagt
    23041 atttttatat tcattggcaa tataatctag ataattttgt gtatccttat ctagaaataa
    23101 ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga tggttgctac
    23161 ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg aacttttcga
    23221 aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag aagtagatag
    23281 acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta cattttcttt
    23341 tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta actgtcttat
    23401 cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat aaaagagctt
    23461 ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct cacgatcttg
    23521 aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt tttgatatat
    23581 ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt atctctataa
    23641 ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat ttattaattt
    23701 gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa tggtatacat
    23761 aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt aattaattat
    23821 atagaaagaa caatctttct atagagttag acatgagagg gactatgctt gatttaaaat
    23881 caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat ggcaattaaa
    23941 gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa ggagtttctt
    24001 aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa acttcgagcg
    24061 gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc tcaagcatta
    24121 actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat tacctagaag
    24181 ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct aatgaacccc
    24241 ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga gcctcagctt
    24301 ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt atacaattga
    24361 taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct acttttgtct
    24421 ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta aatttagcgg
    24481 tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg atcaacacta
    24541 taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata gggttattag
    24601 ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat ccaactatat
    24661 agaaaacgta aattttctat atagttggat taagataacg actatttatt ttatcatatt
    24721 tttactatac cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat
    24781 actactagtt tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat
    24841 acacctacaa cttataaatt gatggtattt agataacacg aaagaataaa aataacagct
    24901 gatagtaaag tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt
    24961 gcttctgaaa acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat
    25021 taaactaagc aataaaaact aatgttcccc tcattctttg aacttctact atttgattta
    25081 tttattctga taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt
    25141 taagatctct tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta
    25201 gaataaactg ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct
    25261 ccaaattata aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa
    25321 agtcagaaga tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat
    25381 atttaaaggg aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat
    25441 ctttcccctg ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt
    25501 atcgaagtga gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca
    25561 ccctctacgg cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc
    25621 aaagaactta tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa
    25681 ttgcttagca gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc
    25741 taaaagtccc aaatggagca actgtattta catgaataaa tttatatact tcccatacag
    25801 ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac
    25861 tcattgaatc aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt
    25921 ctttcaatgg tagatgtgca taggtatttg taaaccactt atataactct cctaactgat
    25981 tccatttaaa ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc
    26041 attttagaat taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat
    26101 ccacttcctc aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat
    26161 atttagtata agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca
    26221 ttatattacc tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg
    26281 atattttaat ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg
    26341 aatgatttat aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc
    26401 taaaaaatac gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct
    26461 tcgtaaatat caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag
    26521 cgttaagaac tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt
    26581 cacgaaagtg gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga
    26641 ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc
    26701 tcatcagatg aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa
    26761 aaagcgattg ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga
    26821 agatcatcct ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac
    26881 aatgatgttt tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca
    26941 aaagaaaatt caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt
    27001 tttttccaaa attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat
    27061 aacttctttt taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc
    27121 gatttttatg actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata
    27181 gtatggacgt ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt
    27241 tagtgaacta cccccactta gc
//
LOCUS       DAYYCM010000036        26814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000036 DAYYCM010000000
VERSION     DAYYCM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.037"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1496
                     /locus_tag="ACHAY1_001568"
     CDS             45..1496
                     /locus_tag="ACHAY1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8593563.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            1489..2211
                     /locus_tag="ACHAY1_001569"
     CDS             1489..2211
                     /locus_tag="ACHAY1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8593564.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            2231..3382
                     /locus_tag="ACHAY1_001570"
     CDS             2231..3382
                     /locus_tag="ACHAY1_001570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8593565.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(3730..4005)
                     /locus_tag="ACHAY1_001571"
     CDS             complement(3730..4005)
                     /locus_tag="ACHAY1_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8593566.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            4113..4877
                     /locus_tag="ACHAY1_001572"
     CDS             4113..4877
                     /locus_tag="ACHAY1_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8593567.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5001..5504
                     /locus_tag="ACHAY1_001573"
     CDS             5001..5504
                     /locus_tag="ACHAY1_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8593568.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            5769..6416
                     /locus_tag="ACHAY1_001574"
     CDS             5769..6416
                     /locus_tag="ACHAY1_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8593569.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      6459..6526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6577..7845
                     /locus_tag="ACHAY1_001575"
     CDS             6577..7845
                     /locus_tag="ACHAY1_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8593570.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(8022..9224)
                     /locus_tag="ACHAY1_001576"
     CDS             complement(8022..9224)
                     /locus_tag="ACHAY1_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8593571.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            9751..10476
                     /locus_tag="ACHAY1_001577"
     CDS             9751..10476
                     /locus_tag="ACHAY1_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8593572.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            10466..11140
                     /locus_tag="ACHAY1_001578"
     CDS             10466..11140
                     /locus_tag="ACHAY1_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8593573.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(11428..11712)
                     /locus_tag="ACHAY1_001579"
     CDS             complement(11428..11712)
                     /locus_tag="ACHAY1_001579"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8593574.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(12006..13313)
                     /locus_tag="ACHAY1_001580"
     CDS             complement(12006..13313)
                     /locus_tag="ACHAY1_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8593575.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVAHHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            13802..14908
                     /locus_tag="ACHAY1_001581"
     CDS             13802..14908
                     /locus_tag="ACHAY1_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593576.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            15499..15699
                     /locus_tag="ACHAY1_001582"
     CDS             15499..15699
                     /locus_tag="ACHAY1_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8593577.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            15846..18635
                     /locus_tag="ACHAY1_001583"
     CDS             15846..18635
                     /locus_tag="ACHAY1_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8593578.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            18682..19209
                     /locus_tag="ACHAY1_001584"
     CDS             18682..19209
                     /locus_tag="ACHAY1_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8593579.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            19230..20420
                     /locus_tag="ACHAY1_001585"
     CDS             19230..20420
                     /locus_tag="ACHAY1_001585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8593580.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            20460..21758
                     /gene="asnS"
                     /locus_tag="ACHAY1_001586"
     CDS             20460..21758
                     /gene="asnS"
                     /locus_tag="ACHAY1_001586"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8593581.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            22692..23366
                     /locus_tag="ACHAY1_001587"
     CDS             22692..23366
                     /locus_tag="ACHAY1_001587"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8593582.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            23363..23704
                     /locus_tag="ACHAY1_001588"
     CDS             23363..23704
                     /locus_tag="ACHAY1_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593583.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            23734..24093
                     /locus_tag="ACHAY1_001589"
     CDS             23734..24093
                     /locus_tag="ACHAY1_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8593584.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            24353..24475
                     /locus_tag="ACHAY1_001590"
     CDS             24353..24475
                     /locus_tag="ACHAY1_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593585.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            24617..26698
                     /locus_tag="ACHAY1_001591"
     CDS             24617..26698
                     /locus_tag="ACHAY1_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8593586.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         8788 a         4641 c         5635 g         7750 t
ORIGIN      
        1 tatagctttt ctgattacat tattattcaa aggtggccaa atggatgaaa aaaggaataa
       61 acatattatt agtggcgctg atgtttctac tggttttttt accagtaaaa gtacaaggtg
      121 cagaaaaaga tgaaatttac gagcggataa aacaaagtgg agaattagtc gttggcctat
      181 ctgcagacta tgcgccctat gaattccatg cgaatgtgga tggtaaggat aaaatcgttg
      241 gttttgatgt atccattgcc gaaaagatcg caaaggatat gggtgtgaaa ctaaaagtag
      301 aagaacttgg ttttgatgca ttattaggtg cgttgaaaac tggcaagatc gatttagtta
      361 tatctggtat ggcggtgact gaagaacgtt tacaagaagt tgacttttct gacccatatt
      421 tcgtggtaca gcaaaaagta ctcgttcgta aagaggacaa aaataaattt aagacgacag
      481 ccgacttttc tggcttagcc gttggggcgc aaaagcaaac cacacaagag gagttagtga
      541 aaaatgaact gactggtgca gagttgacct ccctgcaaaa agttcctgat ctgatcaata
      601 atttgctgaa tgaaaaagtt gaaggtgttg tgctggaaca accagttgca gaagcatatg
      661 cccaacagaa taagaaagta gctttgactc cagtaaaatt tgcgcaaggc gaaaaaacca
      721 cagctattgc gatgtcaaaa aatacaccag aactgaaaaa tcatgtcaat gcgtcaatca
      781 aaacgatcaa tgacgacggc ttgctgaaag attatcaaaa acaagcgaat caattgatgt
      841 tccaagatga tcagtccttt cttcaaaaat acggtccgta ttatttgatt ggaacaaaat
      901 acactatttt attagctttt gtgggtgtat tgtttggaat ggtctttggt agtttgcttg
      961 cactgatgaa attgggtaag aataaatttt taaaggcatt agccattatt tatatcgaat
     1021 acgtacgggg aacacctctc ttagttcaaa tctttatcgt ttacttcgga acaggagtac
     1081 tgggattgga tctatcgaaa ctggtagcag gttgtattgc tgtctcgttg aatagtgctg
     1141 cttatgtagc cgaaattgtc cgtgccggga tcaatgcggt caacaaagga cagatggaag
     1201 ctgcacgttc tttaggaatg gaccagaaag aagccatgcg tttcatcatc ttcccacaag
     1261 caatcaaaaa tattcttcca gctctaggaa atgagtttgt cacgatcatc aaagagtcct
     1321 cagttgtttc ggtcattggg gtatcggaat tgatctttca aacgggcgtg gtacaaggtg
     1381 ctagcttcaa accattccta ccatatgtgt tggtctcttt gatttatttc gtgttgacct
     1441 tcagtatttc ccgtatatta gctttagttg aaaggagaat gggtctgaat gcttaatgta
     1501 aaaaaactgt caaaaagttt tggcgagaat gaagtcttaa aatcaattga tctttctgtg
     1561 aaagaaggag aagtagtggt gatcattggc ccttcaggaa gtgggaaaag tacaattcta
     1621 cgttgtatga atctgctgga agaaccaact tctggtgaaa tcttttttaa gggacaaaac
     1681 attacagccc ctgacagcga tatcaatcaa attcgtcaaa aaatgggaat ggtatttcaa
     1741 agttttaatt tgtttcccca tatgaatgta ctcgaaaatt tgaccatcac acctgtaaaa
     1801 atcaaaaaag aagaccctaa aaaagcaaaa gaacatgctc tatcattatt gaaccaagtt
     1861 ggtctaaagg aaaaagtgga cagttatcct accagtttat ctgggggaca gcaacaacgg
     1921 gtagcaattg ctcgagcatt agcaatgaat cctgatgtga tgcttttcga tgaacctaca
     1981 tctgcacttg atcctgaaat ggtcggtgaa gtgttggcag tcatgcagga tttagctaaa
     2041 aaagggatga cgatggtcgt ggtcactcat gaaatgggtt ttgctaaaga agttgccgat
     2101 cgtgtgattt ttatggctga cggcatcatt caagaagagg gaacaccgaa agagattttt
     2161 gaaaatccgc aaaatagtcg aacacaggat ttcttgaaca aagtgttgta acaaagatgg
     2221 aggaagtaag atggacaaaa gtaagattgc acgccggttt cagcaaccag cagaaaatct
     2281 gttgatgaat attgctattt tggcaaagga aaaagaggat attatcgatt tatcgattgg
     2341 tgatcctgac ttgatcactg atccgacgat tattgaagca gcttatacag atgtaaaaaa
     2401 tggtgctact aattatactg cgcctgatgg cagcaaagaa ttcttgtcaa cggtcgtaga
     2461 tttttatcaa aagaaatatg gattgagcgt tgaatgcaac caagttcgag ctacagtcgg
     2521 agcaatgcac gggatgtatc tagttttaca ggcattacta gatccagagg atgaggtcat
     2581 catccatgaa ccttattttt caccatatcg ggaacaagtc attttatctg gaggcaagcc
     2641 tgtttttgtt cctacctatg aagaaaaggg cttccagttg gatgtggaag atttaaaagc
     2701 agcgattacg gatcgtacaa aggtgatcgt catcaattcg ccgaataatc ctacgggagc
     2761 tgttttttct gaaagcacgt tgaaaaaaat cgctgaagtc gcaatcgaaa atgaactcta
     2821 tatcatttcc gatgagattt atgaagactt tgcctttaaa gagaagttcg taccgatggc
     2881 gcgatttgcg cccgataata cgatcactgt cagtggtgta tcgaaggggt ttgcgatgac
     2941 tggctggcgc ttagggtaca tgatcgcacc agcatacatc aatcaaattg ctgggttgat
     3001 caatgaaaac atcacttatt ccgcaccgac tgcttctcaa agaggtgcaa tctacgcctt
     3061 aaagcactat gaacagcttg taccgccagt tgttgaagta ttcaaagaac gattagctta
     3121 tattgagaaa agagtggcag aaatccctta cttatcttta tcttctgttt ctggaagcat
     3181 gtatgctttt attaatatca gtaagagtgg gctcgattca gtaacgtttg tggaagaggt
     3241 tttagaaaaa agcggcgtac tttttgtccc tggaaaagcg tttggggaaa cgatcgggga
     3301 caactatatt cgactagcag cgactcagcc attggaaatc ttaggtgaag cctttgatcg
     3361 tttagcaaaa ctaaattttt agataaaact gtatatttga aaacagctat gggtatccat
     3421 tagaacaaag agggaaaatg atcctttcga gagttggctt gttgactggg cagcgtcagg
     3481 atttgacaat gaaaaagtgg tagtggaaaa tcaaagtcga ttttccacta ccataaaata
     3541 ttatagactc cattttattg aaccaaaatt tttgaagtcc tcccgctttt acgtatgcct
     3601 atcactggtt actaaactcg ctaataagaa tgtttttttg tcgatctagg atggagagag
     3661 gggcaaactt tttcgatcta catacagaca gtccgatttt tctgacattt cctattcatt
     3721 tctagaaggt cagcctatta caacaaactt ttttctgtcc tccatcatcg gattttcctg
     3781 aaggtctaca tattggacac gtccgctggg gcttggtgat cgtttgactt cttccacgaa
     3841 ttttttgatt gcctcatcgt cgcccgcagc ttcgatcgtg acagagccgt catcttcatt
     3901 tttcactgtt ccggtgatcc ctaattgatc cgctacgatt tttgtcatat agcgaaaacc
     3961 aacgctttgt acacgtcctt gcacgttcat cttgattttg ttcattttat tcaccgttct
     4021 ttcttttatt tttaaattaa tgataccgac ttttttgcta ctgaacaagt gcaatgatct
     4081 aatccatggt ataatcagtt gaggtgaaaa atgtgaaaga gatccaatca gcaaaaaatc
     4141 caagaatcaa agaattaaaa aaattacata agaaaaaata tcgtgaagaa aaaggggaat
     4201 atctgcttga agggtttcac ctgatcgagg aggctgttgc ctcaaatgcg aaggtcaacg
     4261 aactttttat tacgcaacgc ggtctgactg agtgggctag ttggcttgaa gaaaatcagg
     4321 cgctttctgt tacactagta tcagaagaag tgatggcaac tgtttcagat ctaccaactc
     4381 cgcaagggat catggcagtg gtggaagctg aaaaaagaga gataccgaat tttgaaggtg
     4441 gttggttgtt gctcgacaac gtacaagatc ccggcaatgt agggacaatg atccgcacag
     4501 ccgatgcttt tggtctatct tgtgttgtac taggtaaggg aacggcggat gtctacagta
     4561 ccaaagtatt aagaagcatg caggggagta actatcattt accgatcatc caagaggatc
     4621 taacaaaact attgcctatc cttaaagagc aaggatttat aacctatgga acagaactga
     4681 atgaagaagc agtgtccctg aatgatatcc agacaacaga aaattatgcg atcgtgatgg
     4741 ggaatgaagg tcaaggcgta cgtccagaag tgctggcgct aacggataaa aatgtgtata
     4801 taccgattca aggaaatgcg gagtctttaa atgtaggagt tgcagcgggc atcatgcttt
     4861 atcacttctc cgtatagtta aggaactatt aagctttctg cattttgcga aaacctcact
     4921 agaatattac gaacactagt ggtatactaa gttcaatttc aaagataaat ggaattcaaa
     4981 aaggtggcag atcgataatt atgacagaaa aatggcgtga agacgaagaa tatatgtctt
     5041 atataaatga tctattagaa actgatgcag taaagaaatt agcaaattac acgcaacatg
     5101 tccactcgac acgattagag cattcaatca gtgtgtctta ctatagctac aaattagcga
     5161 aaaaatgggg cggtgatgca agagcgactg ctcgagcagg ccttttacat gatttatttt
     5221 actacgattg gcgtacgacg aagttcgatg aaggtacaca tgcgtatgtt cacccaagga
     5281 tcgctgtgaa aaatgctgaa aaaatcacgg agctttcaga tctagagcgt gatattattt
     5341 tgaaacatat gtggggagca acgattgccc ctcctaaata taaagaaggt tatatcgtta
     5401 cttttgtgga taaatattgt gcagtcaaag aagcagctca accaatgaca gcttcaatga
     5461 aacaaaaatg gcaacgatat ttcggtaaag aacaatcgat ttaaggtagt taactaagta
     5521 tttgacccga gtattttgaa aaaaatcctc gggttttttg tttagacatt gactaattgg
     5581 tctttttagt gtttactttg ttgagatgaa aaagtacata accaacataa aaataaaggg
     5641 ggtgatgata agatgcgatg tgtatcttat ttaaaagagt ttgagggctt tatctaaagg
     5701 cactttgata gaaagatcac ttttttcttc aaagtagccg attttgaaag aagaaaggac
     5761 tcaaagtcat ggaaaaaaca atcaaaccat atcaatatta ttttatcaag cgagaagtcg
     5821 agcagctgtt aagcgcctat agttcagtga atgatccaaa gacagtccaa acagtccaag
     5881 cattagctgc ggaaaagatc cacgaggtac ttggtcatcc taatacagct gtaaatgaat
     5941 ttatggaaaa ggtactagac gtcactctaa aaaaagaaaa agcagaacga ctattagagg
     6001 aattgaaagt gatcgttctt ccattcatac aaccgacaaa accgcagatc gataaattat
     6061 tccgaaaagt gaaaaagtta aaacaaccag attggaatca actggattta agggaacata
     6121 cttatattgg ttggaatgat ccaggtagcc aaaaaaaatt tatgatctat tatgaagaag
     6181 aaaaactaaa agggatcagt ggaacattat ctcctacaat caacaaagga atttgttcga
     6241 tttgtcaaag aacttcaaat gtttcactct ttctttctac cacaaaaact ggttcagatg
     6301 gaacatacac gaaaaaagga aattatattt gtcatgatag tgaccaatgt aatcagcagt
     6361 tggtacaatt agctcctttt tacgaattca cagaacgagt gaaacgtgag cggtgatttc
     6421 attgtatttt tctgaaaaat atgctatctt atttggtgaa gtgaataggt gatggtgttc
     6481 gcctttaaac gttgatcgta agataactaa tgacgcctac taaagaaacg gggctacccc
     6541 tttttttagt aggcgttttt ttgaggagga aatgagatgg aaaatcgatt acctgatcca
     6601 ttgaaggtga tcatctatat gatcaaatgg ttattgatca cagcaatttt aggtattttt
     6661 atgggaagtt tatccgcatt ctttctaact agtttgactg atgtgacagc tattcggctg
     6721 gagcatcctt ggttactttt tcttttgccg ataagtgggg gactttttgc ttatctatat
     6781 acgacgtatg ggggcaattc ttcccgggga aacaatttag tgatcgatca agggaatgga
     6841 ggaaatgaaa agattccatt acggctgatc ccactgacct tatttggcac cattaccacg
     6901 catctttttg gtggttctgt tggtcgggag ggtacagcgg tccaaatggg cggggcactt
     6961 gctgagaatt ttgctcgttg gctaaaattg aacaaaatcg agcgtgagat tcttgtcatc
     7021 agtgggatca gcgcaggttt tagctcagta tttgggacac ccttggctgg gacattgttt
     7081 ggattagaag tattggcaat cggaaaagtg agaaccgaag cactgtttcc aagctttttt
     7141 gcagcacttt ttgcgaactt tgtaacggaa agtttcaatg tcaagcatac ccattatcaa
     7201 atgggggcga ttcctgcatg ggacccttca cttttcttca aattgttgct tgcgggcatt
     7261 gcatttggta tcattggctg gctctttagc cgttcgatcg tctggttaaa agcttggtat
     7321 gcaaagcgga tcccccatcc agttttacgc aactttattg gaggagcagt cgtggtactc
     7381 gtagtagtga ttttacagac gcaaagatat ctcggattaa gcttaccttt attacaagat
     7441 gcatttaatg gaagtgctca tccttatgat tttcttggga aacttggctt taccgttcta
     7501 tcacttggcg cgggatacca aggaggcgaa gtgacccctt tgtttgagat tggggcaact
     7561 ttaggtgcga cattagcacc cattcttcat gtatcagtac cttttttagc aggattaggg
     7621 tttattgggg tgttctctgg agcaacgaat acaccgattg cttgctttgt tatggggatc
     7681 gaactttttg gaggtcaagc agccagctac ttctttatga tctgtttgat tagctttatg
     7741 tgttcaggaa atagtggaat ctattctgcg caaaaagttg aagtgaaaaa aggaatcttg
     7801 ttcctaccga tccttggaag aatctcacga caaaaagaga aataaaaaga ggttgggaca
     7861 gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttt aaatttttgt
     7921 gaatttcgct tatttccgaa ggagttgctt ctgctcccac cgtttattcg gagttagagt
     7981 ctgtggcaaa actgattttt agttttgtcc cacgctcttt tttattgagc aaatttgaga
     8041 ataagagcag aaaatactgc aaagccaatc attaagccac agatgataag atggcgtttt
     8101 ttgtgtaaga caaggatggc aataaattct cttaagatag cattcggtaa gtagtatagt
     8161 gcagtttttg caccgattcc gtttgcattg agtttcgctt gtcgcgcata tagtccagct
     8221 ctaaagatat ggtaattatt tgaagtaaaa atcgcgcgat aaggcccatt catttgctga
     8281 tccattattt cttttgaaaa cagcatattt tctagcgtcg ttgtggaatt cgtttctact
     8341 aaaatatcac gctcgggaat ccctttttct aaagcatatt gtttcattgc aatggcttca
     8401 gctacttttt catcggttcc ttttcctcct gacatgagca agactggcgg attcaaagtt
     8461 gctttacttt gtgcccaata aaatgcgatc gccttattga tccttttagc aagtagggga
     8521 gatacgcgtt caccatcaat caagccagcg ccgagaacaa cgatataatc ttggttatac
     8581 cttggacggt tgaactgata caaaattgaa acagtcataa aattaaagaa aacgatgagt
     8641 agataagcaa aaataaacgg aatgacagca aatagagcat ttgcccattg tgggagccaa
     8701 tggactaatg tatagtccaa tatcaaaaag atagtcaaag cgactcctaa caagagagtc
     8761 aaaaggttgg ccaaagaacg ggattctttt ttcatgacta caacaccatt ccaaaataaa
     8821 aaaatgatca aaatgtagat accgattgcc aaaagaatta atattggtat cccgatcaaa
     8881 gccaataacc caccgataaa gatattttgc gtctctatta gtagccaccc tagataacca
     8941 ccaaagacaa tcaaaaacgt attaaaaagt acgccattga ttaacctacg tttttcctta
     9001 aaatagaaaa atgcaaaaat actgaaaaat agcaatggaa tcaaaaaata aaatgtatga
     9061 taaaaaactt ctaaaacgac gaagtaagtg accatcatca taaaagtcca cgtcccacaa
     9121 taaaagaata gagcgggatc gctttggcgc ttgcgccaat agaggatgcc tgacattcct
     9181 aaaatataga taactaaaaa tatcaggtct ctagtaagat tcattgaatg aactcctttc
     9241 gatttgtaaa ttttttctta aaaaagatag catcatttag tagtaaatat atcgaatata
     9301 gaaaagttag ttactaaata gtcaatcagt tttttcacga cgtttctatt ataacgaatt
     9361 atgttgaaaa aagcttttat cgaaaacata aatcttcaaa ttaacagatt ttttagacct
     9421 cgatcatgat gaagactttt gtagatacaa ataactgtaa cattgaaaat gaatgcgaaa
     9481 acgatatttt tttttattgg aaaatatcgg tcaagcaata aaccagttgc ataataaaat
     9541 gttaacggca acaaaatctg tggaaggaat gaaccggatg atttgatgga aaaaacaaga
     9601 tagacaagaa gtatacatag taaaacgtgg aacaagtagg ccagaatgaa aaatcagttt
     9661 ttatccactc gcttttctat acattcactg tcacaaatat aatcgaaagt aattctattt
     9721 tgagataaag tattttatag gagatagtat atgaacaaaa aaagatttaa acgtattgaa
     9781 agaaaatttg cattaactta tgaccaagct aagtatttga aaacactatt tccaaaacag
     9841 gtagttcggg ataaatatag tgatcatttt atttcttcct tgtattttga tacaaacgat
     9901 catcactttt taaaagaaat cactacagaa aagtcctatc gagaaaaatt tagaatcaga
     9961 aaatatggag atcctcagga catatcgcct atctacttag agataaaaag aaagtttcaa
    10021 ggaatgacag ataaacgtag aataaaatta gcgcatcatg agctcgatga tatgttggaa
    10081 aatatcgaat tggcagaaac cacattcagt aatttagaag aagagtatat ttttaatgaa
    10141 atacagtggt tgttcagaag gttggcattg gagcccaaag tatatatcta ttatttaaga
    10201 gaagcatttg taaatagaga ttcagaagat ttgcgaatca cttttgatca aaatatccat
    10261 taccgtctaa tgaacttaac gacctgggaa cactgtacag aagacaatct ggtagcgcca
    10321 gaaatcgatg tgctgatgga agtaaaaacg acaggagaaa tacctgaatg gttacaggta
    10381 ggattggata ggatgggtaa aaaagagata aaattttcta aatataaaca agtctataaa
    10441 agatatatcc aagaggaccc gctctatgtt caatagtgtc ttaaaaaatg agccttctca
    10501 actcacacta gatgatttct ttttatgtat ggtggtgtcg attttgctag gcattctgat
    10561 tgcttacacc catatgtatc aaaacaactg tacaaaaaac ttcatgtttg ccttgattgg
    10621 gttacctact ttagtacata ccgtcatttt gttgatcaac ggtaacttag gcacggggat
    10681 cgctgttcta ggcgcattta acttagtgcg atttcggtct gctcaaggga attcaagaga
    10741 tattttcagt attttttggg caacgtcgat tggtttagcc acagggacgg gatatattgt
    10801 ctatgcacta ttcgtgtcta gtatagttgc tgctatccaa gtcatcatct tcacgcttcc
    10861 aatcaaaaat aaacatacag tttttaatac tgaaaaaacg atcaagctaa aagtttctaa
    10921 atccttgttt ctttcgagta ttattgaaga aaaaattgca gaatatacag aaaactgcaa
    10981 tttaaaaaaa gtaaaaatca ataaagaaac ttgttcctta cactatatag taaaagtaaa
    11041 aacgagtatg cttgaaaaag aattggttcg aacgatcatg gatcatccag aagtcatttc
    11101 gatcactgta ggtatggata aatcgaataa attattgtga taaccaagaa agtacttaac
    11161 aatgaagtaa gaccccagtt ccaaagttta gcggggacac ttcaataaag tactttcttt
    11221 tttgtcttca gcaactactg gtaaaactga gagcaagctt tcgtgagagt acagcgataa
    11281 agatcaacct ccctttttat ctcgagcaat ttccttgcaa ggctgtttcg ttgatcttaa
    11341 acgagttagc aacagtcaaa gatgcgatgg ccataaaaaa attgcattaa gttggctggt
    11401 atctgatagc tgggaatcta aggaagacta tccattctgt ttcttaaatt ccgctagttg
    11461 atagatatca gccaattgtt ccagatgatc aacatcaaag ataaccgaaa gatgtttcct
    11521 tttattccca tttgcttgaa gagtttttat cttctctttg gttccttcac tcaatgagag
    11581 tgtgactaat ttctccctcg ttttttctgg gaaatgaaag gatactttga aaaatccgtc
    11641 ccaaatagaa agccagaaga tcggttttac tttctgagag tgggacaaaa gtaagaaaca
    11701 cttttgtctc attctttaaa aacgaataaa cggtgggagc agaagcaact ccttcggaaa
    11761 taagccaaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttctcgg
    11821 agttaaacac ttctgtccca acctcttgta ttcacctttt gatagccaag cttttccatc
    11881 attataaaac cgcaaatttc aaaaaaaaac gaacaataca aaaagcgtat tgctcgtttt
    11941 tctcttcagt ttctcacacc taaacgactt ttgtctcgac ctctttttgg tgactattca
    12001 tgatgttaaa ataaattact gaaaaaatct gtgacatggc gccaaccaat gacaaaaata
    12061 ttagcttttt caacagtaga agtggtaatg atgtctgtcg aaggctcttg gtttgcttcc
    12121 aaatagccta atttgtcatc agctaattgg atcgttgctg agccaactgt agtgcctttg
    12181 ttgataggag cggtcacttg gtcatccgtt actttctttt tatcaattgt tggagtaatc
    12241 gttaattttc cagtatccat tccattttcg acccagactt ttacagggct ttttagcgca
    12301 acattgacgg aggtttcttt tccatctttc actttgatgt cttttaggct tgggatgctt
    12361 gcatttgctt ttccaagttc tttttctgac cagttgtcat aacaataatc catcaatcgt
    12421 gatgtttcat tgaatcgggc acttggattt tcttcatggt tatctgcatt taaaacgact
    12481 gtgatgattc gtcggccatc tttgttgatc gttccgacaa agcatgcacc cgcaaaatca
    12541 gttgtgccag tttttaatcc atcgacacct tcgtgatagt ttacaaaacc aggtagcatc
    12601 cagttccaat tgaccatttc aaccggtgac tgtgtgtttt cgccgaatgt ttgatttgtg
    12661 gtactcgtga tttttaagac ttcaggataa tctaagatga ggtgatgagc aacaatagcg
    12721 acatctttcg cagacagttg attttcatct gtttcaccag tattctcagg tcgattctca
    12781 cctaggtaag aattgtttaa acctgatgca ttgacgatcg ttgcatcttt gataccccaa
    12841 tcttttagtt gtttcttcat tagctcaaga aatttacttt cactgcctgc aactttttca
    12901 gctaaagcaa ccatggaagc attggctgat tggatgaaag ctgagtcaac aagttctttc
    12961 actgtataat cattttcttt atgcaaagga acattcgata gatccggcac gacacttaat
    13021 ttttctgcat aatccgaaat ggctacctta tcttcccaag ataatttacc ctctttgatt
    13081 tgttcaagta cgatatatag accaatgatc tttgtgatgg aagcaattcc catcgttttt
    13141 tcgccatcct gatcataaag gatttttcca ctttctgcat ctacggcaaa ggcagctttg
    13201 gcatttattt taaatgcgtc gtctgcttga acagttgcgc taggcaagaa agaagtgata
    13261 cataagataa gacttgctaa tagtacaata aaacgggtat tttttttgaa catctttgtt
    13321 ctcctcattt ttaaattgat tcttacaact tagaatatta tcagagagaa caaaaggttt
    13381 caatggttct gaaaaaaacc ttataaagaa caagtcttat taatgaagaa tcatagaaag
    13441 ttatcttttg ggttcattga cacgaacttt gactggctga acttcgggtt cggttaggcg
    13501 tgatgctgca aaaagggcgc ctgctgagag tgcgaccaaa ctaaaaatca tgaataattg
    13561 cataagatca cctcgttctt ttttgttttt ttatagtata gcaagagagt ttgaagctac
    13621 tgttaaggaa atgagcatat aattgatgaa agtatgaaaa aaatatgaag taggacaaca
    13681 caactaaaaa tcgatcaggt atttctctgg tagggattcg ttttattgta ttgataaaat
    13741 gggatgtggt agactttatt tacagataca aagaaatgat ttttttagga ggggtacgtt
    13801 tatggcaaaa aaagaaaaag aattattatc acaattgact tcagaaggta ccaaaggacc
    13861 gtttgtcacg attatgttaa acacccatgt tgcgcatcaa gacgtagaaa aagatcaaat
    13921 caaattcaag aattttgcaa aggaagcgaa aaagcgcttt gaaaagaaat actctgagca
    13981 tgattggtct ccattccaag aaaaaattac tagtttgttg gacgatccgt ctttctggcg
    14041 tagtgccaca acgagtgtag ctgtcatagt atcaccagaa caagtgttta tccaccgttt
    14101 gggtattcct gtagatgacc agtattatgt gagtgatcta ccttatttat tagcgatcat
    14161 caaaaatgga catttcaact attcatacta tttattagga ttgaatcgtg attcgatgaa
    14221 attgtacgaa gtaacgaata aacaagtcaa agaagtggct ttgcctgaag atgcaccaag
    14281 agatgtcgtc actgctttag gatctgaact aacaggaggc gggttgaact acgcatcaaa
    14341 tggtagtgaa ggtacgacgt tccatggagt caatcctaaa gatgaagaag tcgaaattga
    14401 ttgggtcaat tattatcaag cagtcgatga ctatttgaaa gatacatttg aaaatccaga
    14461 gaaatggcct atcttcttat ttgcattacc agaaaatcag acgatgttca aaaaagtggc
    14521 gaagaaccct tattatcgtt ccgatgttgc gatctcagct tcacctgcac aactttcatt
    14581 aaaagaaatt gaagcaggcg caaaaaaact atcgcaagaa ttagctgtca aagaagcaag
    14641 tgactacaat aagttgcttg ataaaaagtt cattgatcaa ctcgttgata ttataccggc
    14701 agcaaaagaa gggaaaatat cccatttatt cattgctaca tctaatttag cagatggttt
    14761 tggcgaagat ccagaaacag aatatgaccg ccgtcaggtg ctgaatgaac tagcagataa
    14821 tgtgatcacg aacggtggtc aggtctttat ccttgaacaa aaagatgcac cagacgaaaa
    14881 gagtttagtt gcgatcttac gttattgagt gaaaactacc aaagtagatg aatggtcagg
    14941 cgttgataga agtgtatagc tccgagagaa ggagtttcct ctgtttcagc cgttggattc
    15001 atcttgagcg agagacaaaa gctagctttt gtctctcgct cattattttt gtgctttagt
    15061 cccattggct ttgcgagggg aagatacttt tctcaaaaaa acgaaccttg cagttgagag
    15121 ggggatagat gcgcatacta atttttatca gttaaaaaat gatgtggatc attagagaat
    15181 tccaaagttc gttcatcctt tttcagataa taaattgaaa ttttttgcaa aagtgaaaat
    15241 agcaaataga aattttctga atcttatagg ggtgatttcg agaaaacgtt cgtttatttg
    15301 caaatatatc ttctaaataa gagaataaat acaaaaaaat aaggaaaaac tctggcaatt
    15361 ttgtaaacgg tatgatatag taagaatgta gtttttgttc ggtaatctgt ggtagcttgt
    15421 ttcaaataaa catcttccag atatacacca ttcaagtaat tgcaaaatat gtgttgatac
    15481 acaaggagga tttttattat ggaaacaggt acagtaaaat ggtttaactc agaaaaagga
    15541 tttggattca tcactgcaga aaacggaaac gatgtattcg ttcatttctc agctatccaa
    15601 ggcgacggat tcaaaacttt agaagaaggc caagcagtaa ctttcgacgt tgaagaaggt
    15661 caacgcggac ctcaagcaac aaacgttact aaagcttaat ttacctatat taaaaataaa
    15721 agtccagaca tttatgtgtc tgggcttttt tgtctttttt tccttaaaaa gcgatgagag
    15781 tatttttctg cactttgtag tataatttca aaagaacatt agaggcagga gaagaggaag
    15841 atattttgaa accaagaact tatgcagtcg ttgatatcga gacgactggg acgaatccaa
    15901 aagaagatcg gatcatccaa tttggctgcg tcatgattga agctggtcgg atcacttcac
    15961 gtttttcgat cgatattcat ccagggcgga agatttcaaa acaaatccag catttaacag
    16021 ggatcacgaa tcaacgcgtc caaaaagcgc cttattttga agatgtcgcc caaacgattt
    16081 acaatctttt ggcagacacc atatttgtcg cgcataatat ttattttgat tataattttt
    16141 tgaaccatga actcatgcga tgcggacttc ctagtttgaa gataccgggc atcgataccg
    16201 tagaacttgc acaaattttc ttgccgacgg aaccaagttt tcgcttagct gatttgtcag
    16261 aaagccttgg ctttattcat gagaatcctc atcaggcgga tagtgatgcc gaagtgacag
    16321 gtcagcttct tttactgatc gaagaacgaa tgaggaaatt gccgatcatc acattagagc
    16381 agattgcacg attaagtagc catacaggaa tggatacgag tcgtttcatt taccacgtgc
    16441 ttgaagaaat gaaagaaaac ccggagcccc ttgatctctc attagaaatc atcgatgggc
    16501 tagctttgca gaaaaaagaa gtggagctat tcacatccgt ccattatggc gagcggacat
    16561 atccaagaaa aaaacaagca aaagaaaaaa tgtttggtaa aacactgatg tatcgaaaag
    16621 aacagaacag gctgatgaat gctgtctacg accatttcac aaaagatgaa tcaaaagatt
    16681 tgatgatcga agcagctaca ggaatgggca aaacgattgg ttatctgttg cctttgagtt
    16741 atttggctac acctgaaaaa ccagctgtca tcagcaccgt ttcgcttgtt ttgcagcagc
    16801 agatcatgga aaaagacatt ccattactga atcaattgct agatcagccg atacaacctg
    16861 tgattatcaa aagctatcgc cactatattg atttacagcg ttttaaaggt acacttgtgg
    16921 aaccgccggg acaaaaacaa tatgctttgt accaaatggc tgtcttagtt tggctaacac
    16981 aaacgaagac cggtgatttg gatgaattac atttaaccaa tctgaaccat gcattctttg
    17041 cagatgtcgc gcacagaggc actgggtttt tagcaaggaa tcagtcattt tacaaacaag
    17101 actttgtgcg tttcttgcaa aagaaaatca aacaaagcaa ttttttgata gtcaaccatg
    17161 catttttagt acaagaaaca cagcgaaaag aaccattgct tccagctagt ccttatttgt
    17221 tgattgatga agtgcatcat ttgccggaaa ttgctgaaaa agtaaatgac aaacgactaa
    17281 gtattaacta cttccataaa caaattcagc aactggaaga gccgaatcag ttatttgacc
    17341 gcatacaaag ttatttgccg aaggaacatg aagccttgcg ctggttgtcg atttatcgag
    17401 aagaactctc tgcactatgc gaagcgcaga tttgcgtggc agagggatta atgaacttag
    17461 taagcagcaa gcaacctaac ggacaatatc cagaagaagt catgatgaca aaagaaatgt
    17521 tggatcagct gccactggaa tctggaacag caatggatca tctgcaactt ttttatcaag
    17581 agatccaaca actacaatca cagatgcgct tgacttttga gattatccaa gaaagttgga
    17641 ccaaccagat gcggatcgat ttagcaagtt tatatagctt atttgatcaa ttagacaagc
    17701 aatacttgct gatggaccaa tggctagaag actggcgaga tcatttgatc cattggttcg
    17761 ttcccgggaa tacagcacag cagatgattt ttcatctcct tgattttcaa gcagctatgt
    17821 tgccgtcaac tgtgtggtat cctcgatatg accatattct ttatattgga ggaacactga
    17881 agatcggacc taatcgacag tatctctcta aacgacttgg cattgaagca gcacctctta
    17941 aagtgattgc ttctccttat gattatgaga aacaggcaag aatctatgtg ccgaaagaag
    18001 cacctgcagt ccatcaagtc agcaatcaag catatatcga ttatttagaa gcagcactca
    18061 caggattgat tgaacaagaa gatcggccga tccttgtttt attcacttct catgatgttt
    18121 tgcaaaaggt atatcagaaa atgcatttgt ctatgctaga aaaaggacgt gaaattcttg
    18181 ctcaaggatt tggcgggagt cgggaaaaac ttttgaaacg cttcattttg tcagaaaaaa
    18241 gcgtcttatt tggtgctgac agcttttggg aaggcgtaga tttacctgga gatgctttgc
    18301 agattgttgt agtcacacgt ttacccttcg acaacccgtt acgtccgatg gtcaaagcta
    18361 ggaatgctta tctggaatca gaggggatca atccttttta tcaggaatcc gtgcctaaag
    18421 cagctttgaa gttacgccaa gcattaggtc gtttgataag gacggagaca gatcgtggcg
    18481 tattacttat tttggatcgg agattagtca ctgcaaaata tggtgcacga atcatccatg
    18541 cattaccgaa aaaagtaccg ataaaagagc tagctataga tgaaatctat caggatatcc
    18601 atgaattctt aaagaaaaat gaagaaggaa gttaaaggtg ccgtcttttt tcatcattaa
    18661 aatctgctat aatgaaaagg aatgaagata aaggagcgac gacccttgaa gaaaaaacag
    18721 acaaaggaaa aaacgattaa tcgcgtgttg cttggcttga tcgtattttt attagcggtc
    18781 atcgtgatca gctcgatttt ttacttgcgt tccagtcgtc cggcggtcca ggcgaaaaaa
    18841 gaagcagtag aaatcgcaaa gaaatatgca aatctcgagg aagccgatca tttttactgg
    18901 tttactcgaa agaaaacgta cttttcattg acgggtacga atgataaagg aaaagaaatc
    18961 gccgttatca ttcctaaatc tggtgaaaaa gtaaaagtat tggatcaaac aaagggactg
    19021 actgaaaacg aagcaaaaca gacaattgct gcggcccatc ctgaaatcca aatcgaaaaa
    19081 gctgctttag ggatgtatga tgatcagcct gtgtgggaag tagtaggaaa agaaacaaat
    19141 gggaaactga attattacct gattggcttt gaatctggag atgaagtcaa gacaatcaag
    19201 ggaatttaaa aggtaaagga atgaatggga tggagttatc aaaaagaaca aaacgattag
    19261 aaccgtctgt cactttggca gctgcggcaa aggccaaggc attaaaagca aaaggcagag
    19321 atgtgctgag tttgactgtg ggagaaccgg attttgcgac accagaaaac atccaagaag
    19381 cagcaatcga agcaatccgc aatgggaaag ctagctatta tacaccaaca gcaggaatcc
    19441 cagagcttcg acaggcaatc gttgattatc tgaagaaaaa ctatggactt gtctatgaac
    19501 cttcacaaac agtcgtaaca gacggagcga aatttgcttt gtataccctt ttccaaacga
    19561 tccttgaccc gcaagatgaa gtcatcattc ctgtgcctta ctgggtcagt tacggtgagc
    19621 aagtaaaact ggcagaagga cttcctgtct ttgtgaaagg ggaagaatcc aatagctgga
    19681 aagttactgt tgagcagcta gaacaagcac gttcagaaaa aacaaaagcc gtaatcatca
    19741 attctccatc gaatccgact ggaatgattt atagcaagaa tgaactgcag gcaatcggtg
    19801 aatgggcagt aaaaaatgat attttgatag ttgctgatga tatttatggc cgtttagttt
    19861 ataatggcaa tgagtttact ccaattgcaa cgatttcaga agcaatcaaa aatcagacca
    19921 tcatcatcaa tggcgtgtct aaaacatatg cgatgacagg ctggcgcatt ggttatgcgg
    19981 tggggaattc tgagatcatc aatggaatga ttgctgttgc ttcccagtca accagtaatc
    20041 cgacagcagt cagtcaatat gcagctgttg aagcattgaa aggcgagcaa gatacagtgg
    20101 aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt atatccgctt gtggcagaac
    20161 tgccgggtgt ttctcttgaa aagccacaag gagcttttta tctttttcct aatgtaaaag
    20221 aaacgctacg catgtgtaaa tatgaaaatg tcacacaatg ggtagaagat cttttagaag
    20281 aaacaggggt agcgcttgtg actggcgaag gctttggggc accagaaaat gtccgcatga
    20341 gttatgccac tgatcgcgct acgttagaag aagcggtacg cagaatcaag caatttattg
    20401 agagtaaaag tcaaaattaa tcctgaagaa attcaaggaa tataaaagga gagtcatacg
    20461 tggaacaaat tcaaatcatc gattcgaaaa accatgtggg agaaacagta acaatcggcg
    20521 cttgggtcgc aaataagcga tcaagcggaa aaatcgcatt tttgcaatta agagacggaa
    20581 cagccttttt ccaaggtgta gttgtaaaaa gcgaagtgcc ggaagaagtt ttccaaacag
    20641 ctaaaaatct acaccaagaa acatccgtgt tagtaactgg agaaatccgt gaagacagtc
    20701 gttcaaaatt tggttatgaa atcggaatta caaatgtgga agtggtaagc gagagccacg
    20761 attatccgat cacaccaaaa gaacacggaa cagacttctt gatggatcat cgtcatttat
    20821 ggctgcgttc ttcaagacaa catgcgatta tgcagatcag aaatgaaatc attcgtgcaa
    20881 cgtatgaatt ctttaatcaa catcattttg tcaaaatcga tccgccgatc ttgactggtt
    20941 ctgcaccaga aggcactacg gatctattcg aaacaaatta ctttgatcaa aaagcttatt
    21001 tatctcaaag tggccaatta tacatggaag ctgctgctat ggctttcgga aaagtctttt
    21061 cattcggtcc gactttccgt gctgaaaaat caaaaacacg acgtcatttg atcgaattct
    21121 ggatgatcga accagaaatg gctttcactc atcaagaaga aagtttagaa gtacaggaac
    21181 aatatgtagc attcttagta caaaatgttt tagatcattg cgaatacgca ttagatgtac
    21241 taggacgaga caaagaagtc ttaaaaactt atacaaaatt gccattccct cgtattactt
    21301 atgatgatgc gatcgaatta ctgaagaaaa atggatttga tgacatcgaa tggggcgaag
    21361 actttggttc acctcatgaa acgttcattg cgaattcatt cgaacagcct gtctttatcc
    21421 taaactatcc aaaagccatc aagccattct acatgaaacc acatccaaca agagaagacg
    21481 tggtgatctg tgcagatatg atcgcaccag aaggttacgg tgaaattatt ggtggaagcg
    21541 aacgggcaac agactatgat ttcttgctag aacaaatccg taatcacggt ttggatgaaa
    21601 aagaatattc atggtatctt gatctacgta aatacggttc tgttccgcac tctggttttg
    21661 gtctaggcct tgaacgaaca gtcacttgga ttgctggtat cgaccatgtg cgtgaagcta
    21721 ttccattccc acgtttgttg aaccgaattt atccataaga ggttgtgaaa tcagtgcctt
    21781 gacctgagta ttagagaaac aaaacggaaa aatagcttct catattttac tgttttttga
    21841 gctaatatcg agggtcagct gattgaacac cgtttatatc aagaggttgt gcgtctgccg
    21901 ttcagctccg agtcacaagg agtaatttca aaaaaacatt ttttcatgtt tgatgactat
    21961 tgtgacttgt gaccaaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg
    22021 aactctcgca gttctttttt tttaaatacg ggataaagga agtttccagc agataagaga
    22081 agaaagcaaa agaaattgct atttgattca caaggttcta taacgaaatc gcaatcaatc
    22141 aaacatatac aaagaaggtt attcaaatgg aaatctgatt tgatgatgct tctgctgttg
    22201 aaataaagca gcatctagca cctgaaaaca aactaatagt aacattcgaa gatggagtag
    22261 gtccgtattt tcagcatgca atgatccata tgtaagttca attcaccctt gaaatcatcg
    22321 gaccagataa agttatggcc aatctatccc aagcaatcta gaagagatat tagtaaagga
    22381 acatttactg gaagtcttag atgagcctat aaatatatgt tctgatggcg tcatcaagtg
    22441 gtctgattga cggaagctta ggtgtgatcg atctctctgg accaaacaat gaaggaatca
    22501 aggggaatcc agcacgttaa gaaaaaaata gcataaaaaa ctagacactt gatgagcgtt
    22561 cattcaattg tttgaaggga ctaggctggt gtcatttttt atacatatat tttctctttc
    22621 gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat tcaaaaagga
    22681 gtttttgaca aatgaaaaaa cgtatattat ttatcggtga cagcattaca gacgcaggaa
    22741 gagaccgagc agaagcaact gatctaggta aaggttatcc acttttgatt gccggaaaac
    22801 taatctccca atatccaacg atggaatttg aatgcatgaa ccgtgggata ggaggagaca
    22861 aaattgcaga tctgcaagta cgctggcgag aagattgttt agctttacaa cctgactatg
    22921 tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag gctatattcg
    22981 gaactgagaa atctgctcag caatttgaat ccgtttatcg aaaattacta aatgaacttc
    23041 aaaaagcggg aatctgcaac atcatcttga tggagccgtt tgtacttcct ataaccgacg
    23101 accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt aaacttgcta
    23161 aggaatatgg cgcattgctg gttcctttag atggcatatt caatgcttta gggattgctt
    23221 atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc ggacatgaga
    23281 tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt ggagataaaa
    23341 atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag aagatctacc
    23401 tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa aaacggcagc
    23461 tgacctaaat gataagaata aactattgct cgttgctgta acaaaagaaa aacggattat
    23521 cggttttgcg caagcggacg tgcaacagtc agaagaacgg gtgggtcagc gaccatcagg
    23581 aaaactaatc caacacacat tagtaacaaa agaaaatacg gaagaaatcc aaaaaaaatt
    23641 tgcagaatat ttgatgctgg aagctaaaaa aagaagaata aaatcaattt ttgcccagtt
    23701 ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa tgtcatctgg
    23761 tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat actttggtgt
    23821 atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctt cctttctttt
    23881 gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat cgttaatatc
    23941 atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag cggcatcctt
    24001 ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact tgctcgattg
    24061 gcgttgatgc cattcggtgc agaaattgta taaaagagaa taataaaggt tagataaagc
    24121 atagagtcag gtgggataca tgtatttcac ctgactctat gcgttttcct gtataagttc
    24181 ttccatgtga cttttacgtc ttttatttat atggggttac tgtttatgga aagttgaaat
    24241 aattttcatt aaagaactct ttctatattt ttgaaagcgt agtggagctt cgtatatctg
    24301 ggaaggacat tttgcaggga ttgaaaaaag atattgtcct atcgaaaaat taatgaattt
    24361 agacaaattg ccagcaacag gcttcaaact tagttgctat cctgtcaaga tcaagaaggc
    24421 aagtgcgggt tggataagag cagtggcaat gatcgaagag aaaaagaaag aataagccaa
    24481 aacatctgta tggaagaaat tttccgatgc agatgttttt cattttttct ttcggatacc
    24541 ttgacatatg aataaccatt catttataat tagttatgaa gagatattca tatgttgagg
    24601 cgaggaggtc attaaaatga aaagttacag attagaaggt ttagattgtg cgaactgtgc
    24661 aatgaaaatc gaaaaaggag ttcagaaaat caatggagtc aaggaagcaa cggtaaactt
    24721 cacttctgga aaactaacga ttgacgcgga ggaagatcat ttggctacga ttgaacaaga
    24781 aacaaaaaaa gtagtgaagg aattagaacc agatgtcaaa gtaacagaaa tagataagga
    24841 aaaagtatct gaacatggaa atgaaaaaga gagaaatacg ctgttccgga ttttattttc
    24901 ccttgcaggt atcgctttat tgctattatt tgattttaat gaactaattc gcttgatagg
    24961 gtatttattg atttatctgc tgataggata tgatgtagtg aagaaggcag tgatgaatat
    25021 cgtcaaagga aaaatctttg atgagaattt tcttatgtct gttgctacga ttggcgctat
    25081 gttgatcggt gaatatccag aagccgtagc agtgatgcta ttttatcaaa tcggcgagta
    25141 tttccaagga ttggcagtca gtcattcaag aaaatcgatt cgcgaattga tggcgatccg
    25201 tccagaaaca gctcacgtgc agacggcgga aggattgatg accgtgaacc ctgaagacgt
    25261 gctgatcggt cagtttgtac ttgtgaagcc tggtgaacgt gtaccgttag atggaacgat
    25321 catcgaagga gaatcattag tcgatacgtc agcacttaca ggagaatcag tccctaaatc
    25381 tgtatatgtt ggtgaaacag tattaagcgg atttatcaat aaaaataaac catttttagt
    25441 tcaagtagaa aaatcttatg aaaattctac tatcagcaaa ttattagaat tagtagaaaa
    25501 tgccagcagt aaaaaagcac cagctgaaaa tttcattact aaattcgcgc gctattacac
    25561 accagtcgtt gtaggattgg ccgttttatt agctgtttta cctccacttg ttgtttctgg
    25621 agcggcgttt tctgaatgga tctatcgagc attgacattt ttagtgatct catgtccatg
    25681 tgcattagtg atttccgtgc cacttagttt ctttggcggt atcggaggag caagtaaaat
    25741 cggtgtatta gtaaaaggtt caaattactt ggagttgctt gcgcaaaccg agacagtggt
    25801 attcgataaa accggcacat taacgaaagg cgacttttcg atccagacga tcgatacaaa
    25861 aatcgatcca aagatattca tgcaatatat tgcgagtgca gaacagtttt ctacacatcc
    25921 gattgctcaa tctgttttag aaggctacgc aggtccgctt ttaccaacag ctaatatcca
    25981 ggaatttgca ggtgaaggga tcttagctga agttgatggc aaacaagtat tagtaggtaa
    26041 tcacaaattg atggaaagat ttgaaatctc ttttccttca agtcaagaaa tcggtacatt
    26101 gctttatcta gcaatcgatc aaagttacag tggttatctt gttattgcag ataccttgaa
    26161 agaagatgcc gtagacgcat tggttcaatt aaaacaggct ggtgtgaaaa acacagtcat
    26221 gttgacgggc gactcgaaaa aaattgctga tcatatcggg aagcaagtag gcgtggacaa
    26281 gatatatagc gaattgcttc cagaagataa agtgcagcgg ttagaggaaa tcttacaaag
    26341 taacaataaa aagaccgcat ttgtcggaga tggaatcaac gatgcaccgg tgctagcaag
    26401 agcagatgta ggaatcgcaa tgggtggatt gggaagcgat gctgcaattg aagcagcaga
    26461 tgtagtcatc atgaatgatc aaccatcgaa gattgcagaa gcaatccatt tagcaaagaa
    26521 aacattgaaa atcgtcaaac aaaacattgt ctttgctatt gggataaaaa ttttggtact
    26581 ttccttaggt gcttttggtt ttgcatcaat gggagatgca gtatttgcag atgttggggt
    26641 gacagtatta gctgttttga atgccatgcg aagcttgcat gtaaaaaata aacgttgatc
    26701 aatgaagagg acgaatgaac gtcctctttt ttttatatcc ttttcttaat gaaaaatgta
    26761 aagaaaatag ataggattat aaaataacta atgtttttaa ttttgcagaa aatt
//
LOCUS       DAYYCM010000037        25502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000037 DAYYCM010000000
VERSION     DAYYCM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.038"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHAY1_001592"
     CDS             133..3660
                     /locus_tag="ACHAY1_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8593587.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHAY1_001593"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHAY1_001593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8593588.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHAY1_001594"
     CDS             7448..7789
                     /locus_tag="ACHAY1_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8593589.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAY1_001595"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAY1_001595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8593590.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAY1_001596"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAY1_001596"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8593591.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAY1_001597"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAY1_001597"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8593592.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHAY1_001598"
     CDS             13000..13737
                     /locus_tag="ACHAY1_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8593593.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHAY1_001599"
     CDS             13751..14566
                     /locus_tag="ACHAY1_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8593594.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHAY1_001600"
     CDS             14579..15226
                     /locus_tag="ACHAY1_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8593595.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHAY1_001601"
     CDS             15242..15898
                     /locus_tag="ACHAY1_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8593596.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHAY1_001602"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHAY1_001602"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8593597.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHAY1_001603"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHAY1_001603"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8593598.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHAY1_001604"
     CDS             18240..18758
                     /locus_tag="ACHAY1_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8593599.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAY1_001605"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAY1_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8593600.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACHAY1_001606"
     CDS             complement(19645..20565)
                     /locus_tag="ACHAY1_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8593601.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACHAY1_001607"
     CDS             20691..21743
                     /locus_tag="ACHAY1_001607"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8593602.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHAY1_001608"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHAY1_001608"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8593603.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHAY1_001609"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHAY1_001609"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8593604.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACHAY1_001610"
     CDS             complement(23383..24144)
                     /locus_tag="ACHAY1_001610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8593605.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACHAY1_001611"
     CDS             24333..25427
                     /locus_tag="ACHAY1_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8593606.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAYYCM010000038        24424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000038 DAYYCM010000000
VERSION     DAYYCM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.039"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHAY1_001612"
     CDS             complement(53..787)
                     /locus_tag="ACHAY1_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8593607.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHAY1_001613"
     CDS             1114..1524
                     /locus_tag="ACHAY1_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8593608.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHAY1_001614"
     CDS             1517..2218
                     /locus_tag="ACHAY1_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8593609.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHAY1_001615"
     CDS             2218..3804
                     /locus_tag="ACHAY1_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593610.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHAY1_001616"
     CDS             3823..4044
                     /locus_tag="ACHAY1_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8593611.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHAY1_001617"
     CDS             4041..4802
                     /locus_tag="ACHAY1_001617"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8593612.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHAY1_001618"
     CDS             5182..5691
                     /locus_tag="ACHAY1_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8593613.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHAY1_001619"
     CDS             complement(5766..6305)
                     /locus_tag="ACHAY1_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8593614.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAY1_001620"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAY1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8593615.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHAY1_001621"
     CDS             7318..8169
                     /locus_tag="ACHAY1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8593616.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHAY1_001622"
     CDS             8197..8832
                     /locus_tag="ACHAY1_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8593617.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHAY1_001623"
     CDS             8852..9625
                     /locus_tag="ACHAY1_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8593618.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHAY1_001624"
     CDS             10033..10830
                     /locus_tag="ACHAY1_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8593619.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHAY1_001625"
     CDS             10808..11221
                     /locus_tag="ACHAY1_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8593620.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAY1_001626"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAY1_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8593621.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAY1_001627"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAY1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8593622.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHAY1_001628"
     CDS             15998..16558
                     /locus_tag="ACHAY1_001628"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8593623.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAY1_001629"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAY1_001629"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8593624.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHAY1_001630"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHAY1_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8593625.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAY1_001631"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAY1_001631"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8593626.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAY1_001632"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAY1_001632"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8593627.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAY1_001633"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAY1_001633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8593628.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHAY1_001634"
     CDS             21905..22483
                     /locus_tag="ACHAY1_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593629.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHAY1_001635"
     CDS             22535..23017
                     /locus_tag="ACHAY1_001635"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8593630.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHAY1_001636"
     CDS             23134..23508
                     /locus_tag="ACHAY1_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593631.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24424
                     /locus_tag="ACHAY1_001637"
     CDS             23938..>24424
                     /locus_tag="ACHAY1_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8593632.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FM"
BASE COUNT         8006 a         4389 c         5213 g         6816 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atgg
//
LOCUS       DAYYCM010000039        24257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000039 DAYYCM010000000
VERSION     DAYYCM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.040"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAY1_001638"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAY1_001638"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8593633.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAY1_001639"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAY1_001639"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8593634.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAY1_001640"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAY1_001640"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8593635.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHAY1_001641"
     CDS             complement(3373..4680)
                     /locus_tag="ACHAY1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8593636.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4680..5261)
                     /locus_tag="ACHAY1_001642"
     CDS             complement(4680..5261)
                     /locus_tag="ACHAY1_001642"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8593637.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5377..5474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5833..7125)
                     /locus_tag="ACHAY1_001643"
     CDS             complement(5833..7125)
                     /locus_tag="ACHAY1_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8593638.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7122..7745)
                     /locus_tag="ACHAY1_001644"
     CDS             complement(7122..7745)
                     /locus_tag="ACHAY1_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593639.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7748..8584)
                     /locus_tag="ACHAY1_001645"
     CDS             complement(7748..8584)
                     /locus_tag="ACHAY1_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593640.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8601..9488)
                     /locus_tag="ACHAY1_001646"
     CDS             complement(8601..9488)
                     /locus_tag="ACHAY1_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593641.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9504..10787)
                     /locus_tag="ACHAY1_001647"
     CDS             complement(9504..10787)
                     /locus_tag="ACHAY1_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593642.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11470..12153)
                     /locus_tag="ACHAY1_001648"
     CDS             complement(11470..12153)
                     /locus_tag="ACHAY1_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8593643.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12171..12788)
                     /locus_tag="ACHAY1_001649"
     CDS             complement(12171..12788)
                     /locus_tag="ACHAY1_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8593644.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12798..13622)
                     /locus_tag="ACHAY1_001650"
     CDS             complement(12798..13622)
                     /locus_tag="ACHAY1_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8593645.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13615..14175)
                     /locus_tag="ACHAY1_001651"
     CDS             complement(13615..14175)
                     /locus_tag="ACHAY1_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593646.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14349..15305
                     /locus_tag="ACHAY1_001652"
     CDS             14349..15305
                     /locus_tag="ACHAY1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8593647.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15610..16566)
                     /locus_tag="ACHAY1_001653"
     CDS             complement(15610..16566)
                     /locus_tag="ACHAY1_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8593648.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16670..17272
                     /locus_tag="ACHAY1_001654"
     CDS             16670..17272
                     /locus_tag="ACHAY1_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8593649.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17360..18859)
                     /locus_tag="ACHAY1_001655"
     CDS             complement(17360..18859)
                     /locus_tag="ACHAY1_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8593650.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18875..19399)
                     /locus_tag="ACHAY1_001656"
     CDS             complement(18875..19399)
                     /locus_tag="ACHAY1_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8593651.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19697..20446
                     /locus_tag="ACHAY1_001657"
     CDS             19697..20446
                     /locus_tag="ACHAY1_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8593652.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20437..21222
                     /locus_tag="ACHAY1_001658"
     CDS             20437..21222
                     /locus_tag="ACHAY1_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8593653.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21191..21907
                     /locus_tag="ACHAY1_001659"
     CDS             21191..21907
                     /locus_tag="ACHAY1_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8593654.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22009..23028)
                     /locus_tag="ACHAY1_001660"
     CDS             complement(22009..23028)
                     /locus_tag="ACHAY1_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8593655.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23047..24048)
                     /locus_tag="ACHAY1_001661"
     CDS             complement(23047..24048)
                     /locus_tag="ACHAY1_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8593656.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7187 a         5220 c         4224 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccat
     4681 tatgcgtcct cctcaagaaa ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga
     4741 gaaacgactc tcaatcccat ttcttcaagt aactgacgtc catcttggaa agacttttcg
     4801 atgacgatcc cgattccttc tacttgcgct ccagcttgct ggcataattc gatcaatcct
     4861 tttgccgctt gtccgtttgc caagaaatcg tcgataatca atactttgtc ttctgaagat
     4921 aaaaatttac gagaaataga aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa
     4981 gctgtcaata attcttcatc catcgtcaaa ctttttgctt ttctagcgaa gatcattgga
     5041 acatctaatt ctcttgcagc aaataaagct ggtgcgatcc cagaagcttc aatggtcaca
     5101 actttcgtga ttccttgttc tttaaatact tctgcaaacc gttttcccat ttgttccatc
     5161 aatacagggt ctacttggtg agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat
     5221 atacgtccgt ctttttgaat acgttcaact aattctttca ctttatttgc tcctttatca
     5281 tttattaata ccgaaataac cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa
     5341 aacaccctcc actttatcat aagaaaagaa gggtgaaata gccaatcttt tacttatagt
     5401 ccagcattta cggtactggc tagaaactgt catccatatt atgacgatat ataagtaggt
     5461 atgtaaaact aataatagtg taaattcatt ttcttgcaac tgttttgcac gtgaaggcag
     5521 tttgccatat ttgaataaaa aatgttgttt caaatattga aaacagtcta acactacttt
     5581 ctgaaactga caacgtggtt tacttatttt tttgtaaaat ctaatgattt ttaatgcatt
     5641 attccttttt gttcgggttt aaatagttat ttttattttc tcctatctaa aaaagcagcc
     5701 cacataaaaa gaaggcatga caaaaagcat gtcatagccc ctttttccga ataaatagtg
     5761 ttcaaaagac agcctatcgg caataaggca agattttata aactacggga agtaatttcc
     5821 gtaaatctat tcttattctt ttaagctgac aagctcgtca atcactactg cttttcctga
     5881 agcaatagaa tgatttgctg cgattcctgt caaaatcgac aaaataccat ctacgtgagt
     5941 tgctgcccga ttccacggat cttcttcttt ttcttcacca aatagatcat ctaataaacg
     6001 cggatctcct ccgccatgtc cgcccgcttc ttgttcgacg attacctcat atggctcacc
     6061 gaacatcgga tagacatgga tggactgaag aacggctgct ccttcatcct ttttatctcc
     6121 acctgcattg atgtaagact tttcgatcac tttcatttcg atccgtcctt tgctgccatt
     6181 gaaagaaaca ttgaatcctt cccacggcat atacgcgttt agcgaatacg tcaaaatcgc
     6241 accagtttca tatttgaccg ttacgcctag tgtatcttca atagagatat catcggaaaa
     6301 tacactgcgg tcacgaatat atccactgtc tttttctgct tctaaataca tttctcgcaa
     6361 ttcctcattg gctgacaggt ctatagcaaa tggatcagct ttagctagtt cgcttccata
     6421 gcatcgactg taaaatattt ctactccgcg tttctccgca ttatgcatcc cataaaagtt
     6481 taatccaccg aatgcaaata ccttttgggg acggctgttc aaccaaaaat tcactagatc
     6541 aaaatgatgg gtagatttat gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca
     6601 tcttctgaaa taatctgccc cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa
     6661 cacatcacca atcacatcac tttctatcag ttcctttatc ttcgtgtgat acggtgcata
     6721 acgataatta aaagtgactc gcaggcttct tcccgtctgt ttttggaccg ttattatttc
     6781 ttgagctttt tcctcatcga tagtcagagg tttttcgcaa ataacatcat atcctttttt
     6841 cattgcacga atgatataat catgatgcgt tcgatctact gacgtaataa tgacttcagc
     6901 cggccgtacg tcatccagta gttgctcaaa ttgtgatggc atacatgtct ttactttttt
     6961 ctgtccatac ttgttcacaa gtatccgatt tgcataatcc atccttgttt gagatacatc
     7021 acaaaaaccg actagttcac aagtttgatg atacgtagag gcaattgcct cataaaaaaa
     7081 acgcgctctc ccaccaagtc cgacttgtac atattttttc ttcatttaaa gtcattctcc
     7141 ttttctttta agcaaaatac tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt
     7201 aaaaaatcat gctgtataca ccagaacagt ccatacaaaa ggcataaata ccacagaaac
     7261 cccttgacgt tttttgacaa cagccagata ttccttttga tcaaataaac aagactatct
     7321 gctttatctt ctttgcagat atcaattagc gtactaaaaa aactatggga tacaaaaagc
     7381 aacagacaca atagaaaagg aatcagatac caagctccaa tctttcccac taataaatac
     7441 caatcaactg caaaaataat gacaaacagc tgaaacaacg taatgatgaa acacatccgt
     7501 ttgatttttc ctttcctcca acataccttt ttttctgcca agtaatggac agatagataa
     7561 taagaaggat acatcatcaa aaaataaatc agatagacaa cacgagcaga cagcactaat
     7621 cctaaagtga acaaacatcc tagaactaaa gcgaaaagag cattactgat aagactggtt
     7681 aaaaaaatcc gacagacata gtagttgagc ttgtaaagaa acatctcttc tattggcttt
     7741 ttcatattca gcccttcaga ccgcttgtac tgatcccctg gacaagatat ttattgaaga
     7801 ataagaaaat cagaaaaacc ggtagcagcg acaacgtaga catcgcaaac atactcgcgt
     7861 aagtcgtact agatccagcg tctgcaaata atttgatggc aatcgatacc gtgtaagatc
     7921 tcggcttgtt taaataaatc aatggtccca tgaagtcgtc ccatttccaa taaaactgga
     7981 tgatgatcgt cgtgatgatc gaaggtttca gcaaaggaaa aataattttg gtaaagacac
     8041 tatacttgct gcatccgtca ataatcgctg attcatctaa ctctttgggg atgctgacca
     8101 tgaattgcat gatctggtaa atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg
     8161 gcacatacgt cccaaccaaa ttcagccgat tgtaaataat aaactgcgga atcaaaatca
     8221 cttgtgcagg aatcatcatc gttactaaca tgacgacgaa ccagaatctt tttcctttga
     8281 aatcgattct cgataatgcg taagcaatac aagtcgagct cagaactgtc cctatcgtcg
     8341 aaactgcaga gatgacaaac gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc
     8401 ccgcccatcc ttttgcgtaa ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac
     8461 cactcataat ctcttgatcc gttttaaaag aacctgcgat catccataag accggataaa
     8521 tcatgactag acctaatagc aaaataaaac cgtattgaaa ccatttcgtt actgtattat
     8581 tcatttcttc ctcctctcat tcacacaacg acccattttt ttgatgattt gaaaatcaga
     8641 aaggtgacag ctcccataat caacagcatg atccacgaaa tagcactagc ataccccata
     8701 gaattaaaag aaaaagcttg gttatacaca taaagagcat aaaagttcgt ggcatcattc
     8761 ggaccacctt gggtaatgat aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc
     8821 ataaccaagt tatacaaaat gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc
     8881 tgccatttgt tcgctccatc gatctctgcc gcttcataat acgagcctgg gatctctttg
     8941 atacctgcag caaagatgat cattgccgaa ccgaattgcc aagcagacat caaaacaaga
     9001 gggatcatga ccagtttttg atcgctaaac caggaaagcg caggtgcgcc taatcgttcc
     9061 aggaaaatat tcactaaccc gtttttagaa aacaactccc gccaaactag agcgatagaa
     9121 atactgccac cgatcaaact aggcacataa tacaaagaac gaaagaaagt cgttcctttg
     9181 atattcctcg ttaaaagata agcaatcaat agaccaaaga tcagtttcac aggtacagat
     9241 gcaaatacat agagaaaagt gactcggatg gagttcaaaa aacgggcatc actcagcatc
     9301 tttatataat tatccaagcc gatccatgag actggatttc ccaaacgata atcggtgaac
     9361 gaataataaa gtgacaagat catcggtatc actgttatca atagaaatcc taggatgaat
     9421 ggagaagcaa atacgtaacc gaccacattg tcttgatcca agaaccgttt tagtttttct
     9481 tttttcatgc cttcctcctg ctgctatcct agaatgtttg acgcaaactc ataaagactc
     9541 tctgctgctt cttctgctgt tacttcttca taaatgactt tttcaactaa aagattgtat
     9601 tggtcttgaa tttccggatt caaagcacta tcaaagacat tcacttcgcc gttgtccata
     9661 tcggaaatca tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg
     9721 agctgttctc gaatattcga catgattggt acaccacgtt ctccttttaa aatttcattg
     9781 gcttcttcac tgttttgaaa gaaatcaata aacttagcag cttcttcttt atgggctgag
     9841 ctttttgcca tactcagcat ttgcgaagaa cgtaaaggga taccagattc tccatctgct
     9901 tttctgcgag gtggattaat caactgaatc tccatatctt caggagcccc tgacaatatt
     9961 tctgtcaact ggttacttga tagaaacacc attgccgctt ctctatccct aaccgcctga
    10021 aaagacagat ccgtcgtact tgcagctcca ccacctggtt ctggatgggc ccctttgtct
    10081 accagatttt tacgcagctg gaaaaaatct gttagatagt ctggattttc aaatcccaag
    10141 ctgttagata gatcttcccg attataaaaa ttcaatcctt tctcaacttg tggtatttga
    10201 ataatacagc cagctataaa atcttcgaat cttgaaatag catagattcc taattcttca
    10261 gtgatttgat agctgatttg ttcaaattca tcccatgtcc aatttgaagc tggttcaggg
    10321 atatttaatt gttcaaatat atttttattg taagcaatcg caaacgaatt caccccgtta
    10381 gacagtccaa ctaattgccc gtcgtaagtc gtcgttgcta aataggattc atctgcatta
    10441 gaaatatcaa ttatcccttg ctccacatat tcatctaatg gttcaatctg attgatgtat
    10501 tgaggaaatt gattgttcag ttggaaaatg tcccagatat catctgatgc tgctagtgta
    10561 cctaattttg tgtaatatcc ttcataatca taaaattcat aatcgatcgt cacatgggga
    10621 tttttttctt cataaagctc gattactttt actgttgcat cgtgtcggac ttgcgaaccc
    10681 caccaagcca tgttcagcgt gatttttttg tcatcttctt gcccacaagc cgtcaagaag
    10741 aataacataa cacttaaagc taagatgatt ctcattcttt tcttcattct ctgcacctct
    10801 acttccattg ttatttttca atatactgta tttttataca gaacatattt attaaacgat
    10861 ctttttcaag gggaataaaa accaagactt gccttatcgt tctgcctagg atgaacaact
    10921 caatactttc aagaaaaatt agatagtgct gattcttctt tcatcgctaa aaacacttct
    10981 tgcttcaatg atgggaggat aattacgcaa agaaaaaagg atatcagaca actgcccaat
    11041 atccttgaca tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa
    11101 aaataactag taattctgta tgaaattagg tacaatatac acgtcgatgc acaagcattg
    11161 actatggcaa cttgattggt cagttcccgc tgacttttca agccccgaca atttagctgc
    11221 tactaaattg tcgggtgcct tttttagttt ttattcgttt ttcattctat agaaagcgct
    11281 tcaataaatc aagacatttt tttgttagaa tgctaataat aacaaaaggt tttaactctt
    11341 cttatgtaca ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa
    11401 atagtactgg agtacaaaag acgaggaaga gccactatct gctcttcctc gtcttttgat
    11461 tctcacctgt tattcatgat tctttttctc agtttcccga tatttttcca ttttacgact
    11521 aaaaagctct ccgctacttg gtggcgtata tgtgatggca tttgctcctg cttgaatggt
    11581 ctcagcaata ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt caggatattt
    11641 tttacgaaaa tggcgtactg cttctgcagt ttctttacct gcactgatat tgataatcga
    11701 aataccagct gctaactttt catctatttc tgtatatttc gacgttaccg tactgataac
    11761 tggaatatca actactcgac aaacttcttt tactgtctct aaaggcgtag gaccgttcaa
    11821 aacgacccct aacgaacctt gcgcttctgc gaacatgctc atgtaggaag aacgagcacc
    11881 ttgtgtcaaa ccaccaccta caccggagaa aactggaatg tcagctgcct caataatgct
    11941 tttagtaatc gctggatgag gtgtaaaagg atacacagca attactgcat tcgcatttgt
    12001 attacgtatg atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat
    12061 cttgatcccg cttgcttcta aaatgatttc cggtactctt acgatatctt ttctcaattc
    12121 cgtcatgatt tctggcgtaa attgttctgc catcagccac ttctcctttg ctatctccgg
    12181 tcatactgat tcaattgata cgtctcgatg acctggatga ttttttgcgc ataaccgggg
    12241 tctgtcgcat aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta
    12301 atcacttttt catacagcgc aggattccaa tctactcctt ggacgaacag ttgtgtatgg
    12361 tcatccatgg actgttccca tgaatcatac actttgaagt ctccttggat agttatccat
    12421 tcttcgttaa caaattcctt tgtttccagg cttactttct tctgatcccc ataagccttg
    12481 atcccgaaaa gattattgta tttgctagcc aaggtacttt ttccccaatt tgattctaat
    12541 atagcttgtc ctaaaatgat actaggaagc actccataac catcttgtag ttcttgggca
    12601 tgcggaacaa gccgttcaat aaattgctga tgggattgcg cttcattttc ttgctgatta
    12661 tcagtaaaag gagaagacag attcttcaag gaaaaaacaa aggctaagag tatcactgct
    12721 acaccggcaa ccactgctgg ccaacggata ttcttgcgct tttgcttttt ccactttttt
    12781 ttcgccatga tcaattccta cttttcattt aattcattca gatattcttc tagcgtcaat
    12841 tggtgtttgg tgatatactc agcgttttct ttaccgacat aacgtaagtg ccaaggttca
    12901 tatgtgatct tagttatatc ttcgtgtcct tttggataac gaacgataaa tccatactgc
    12961 gcagcattat ctgcgatcca tttagctcca ggctgatctc cgtaacttgc atccaataca
    13021 gtagaaggat aactgttgta ccagttttga tcgacgacat cgactgccag acctgtgtgg
    13081 tgttcgctgt atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct
    13141 gatacacctt gacgactcat cacatcttgg acattttgtt cgaaaacttg ttgttgggat
    13201 gatacagacc ggtaagctga gaccatcact aatggaaatc cagctgcttc agcagccttg
    13261 gcaaatgctt catattctgg agcaatgcgt ttgtcgatca agtatccgtt acttaatgat
    13321 gccaattgag tagactcatc aatttctttt tctaatttgt gatctggacc aaccaaaact
    13381 agcttccaat cgtctgtttt aacatctggc aattgtttgg acgtacgatc ttttgtttct
    13441 ttgtccttta ccttgctgct ggttgtttga ctgttctttt ctttactagc agttgttttg
    13501 gtgcttgttc gttcgactgc ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa
    13561 ataacatagc ccattgctgc gatcataatc acaactgctc taaatcctaa aaatttattc
    13621 acttgaaaaa tcctttcgtt gttcttcacg ttccccattc gcctcatgaa cgtaagtttc
    13681 ttccagattt tcattcaccc aatcgagaag ttcgattttt tctccttcga tctgtttttg
    13741 aacatctgct ggcagacgaa aattcaaaat atcatcatag ccccattcat catattgaat
    13801 acttactttc aagtcaaggt atctaccagc ctgctgatta tttttcaaag ggatcagctc
    13861 gacttttgct gctttgctgc taagccattt ttgcgcgatg cgtgttttga tttgcttgta
    13921 ctctgcaacg gcaacgtttc gtttttcggg aaaaataatc gtttgacgca aaatcttttg
    13981 cgttagcatc cattcataat cagtgaaaag attttttact ttctgcatcg cttcagatgg
    14041 cagatttttc tcgccatctt tccatttttg ccattgttct ggactgatca gcaagtattc
    14101 atctaaaaaa tttttttcag tttcaaactg ttctaataat acactcataa taagatcaat
    14161 atatacttga tgcacgttta tcccctcctt ttaactattc tacttaagtt ttgatcaaaa
    14221 agcacctctt aaatcgctgc tttcattttt tttaatgttt caagtcctct cctctttatt
    14281 cttcgtgatt tttgttataa taatagacag acaagaaact caatccaact atgaaggagc
    14341 gttttcagat gaaaaaatat caatggggaa tcgttggtct tggtaccatc gcacatgagt
    14401 ttgccgaaag cttcaatcaa gagacgagcg aactaacagc agttgcttcc agaacttcag
    14461 aaaaagcgga aaacttcgct caccgttaca atattccaaa agcttacgga agctatcaag
    14521 aaatgttaga cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat agacagcata
    14581 tcgaccatat cttggcagca ttggaagcag gcaaacacgt tctatgtgaa aaagccatca
    14641 cgatgaataa aaaagaactg gctgacgcta tgcgcttagc agaagaaaag aatttgatct
    14701 tagcagaagc gatgacaatc ttcaacatgc cactttatca gcagttacgc tcaataatgg
    14761 atacaggaaa actgggggct ttaaaaatga tccaagcacc tttcggcagt tacaaagaac
    14821 cagaccctaa aaaccggttc ttcaatccag aattagctgg aggagctctg ttggatatcg
    14881 gtacgtatgc tgtatctttc gctcgtttct ttttgagctc tcaacctgaa gtagttgcct
    14941 ctacaatggt tccttttgaa acaggcgtgg acgaacaatc cgtcactatc ttgcgtaaca
    15001 aagaaaatga acttgctgct gtcagcttga cttttcaggc aaaaatgcca aaagtcggta
    15061 tcgttgcctt tgagaacggt tatgtgacga ttgctgatta cccgcgtgct gatcgtgcgg
    15121 agatcttatt tacagatggt acaaaggaat tcatcgaaag cgggaataca tctgaagcaa
    15181 tgaattacga aataaagaat atggtacaca tgattgaagg tgacttgccc aatcgctctc
    15241 tcttcttgac aaaagatgtc atcgaaatct tggatcaaat gcaagttcta tggaaaaata
    15301 tgtaatttta aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca
    15361 aaaatagctc ctcctatttt tcgtttggct ggacttatta ccgcagagga aagcttttga
    15421 acaccgttta agaaagaggt tgtgagccta ccgttcagct ccgagtcaca aggagcaatt
    15481 tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttgacagg
    15541 agaacaccgt ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata ttagctgaat
    15601 tcacaacctc tatttttgga ttttttgttg ggagaattct gccttgattt caataaaact
    15661 tttaaaagac tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag ggcgactagt
    15721 cggcagaggc atttgccgat acaatacata aacttcttca atacgtgtta aaatttgtct
    15781 gccatcattt gactctgaaa atgtttctgc agtataaatc aataaaccat agatatgttt
    15841 tccataataa gctggttgct ggattctctc tagattttcc agcatgtctt taatcacatt
    15901 ggcctgtgct cttctcatag agaagtaagt ttcgtagtat agcggctgtc tgagccagta
    15961 gttttcttga tgttctctgg catcttcttg gctttcgcga atgaatgtca gtaaatgttc
    16021 gcatttgatc tgcacatctt ctttattttc tgataaaaga aattctgcca tttgtctcaa
    16081 aatatttcga aattcttttt cgatctgtat ttggttttcc ttcagctgct tcgtacgatc
    16141 tggcatgtaa acatttgcaa gcaaagcaaa tactaatcca atcgccatta atcccgcttc
    16201 attgccgatc agctgccaag acatgttttc ctccactaaa taatgagtga ccaaaaccga
    16261 atttactact atcccttcgg ttaatttgaa gcgagaggca agcgggatga acaggaataa
    16321 aaaaagactg aagccaaaaa tagtataccc aacagcaaaa aaactgaaca gtgctgttgc
    16381 cgtagctaat ataaaggctg cgattcgatt catgcccgta cgcaatgtag agcgcttggt
    16441 atttcctaca cttaataatg caatgatccc tgcagctggc caatacaaca gcgataagct
    16501 acccgctgtg accatcgaaa agaagacact gatcactgtt ttgactgttc gcattcctat
    16561 acgcatttac ttcccccttt tttattttaa ttatacttga ataatgaaaa actagctatc
    16621 agacaaaagt ttaggtatga tagtaaaaag accaaaaagg agaatctcta tgcaatttgg
    16681 ccgttttcga ctaggtatgc gtacgatcaa aagtgcctta gctgtttttt tatgtatctt
    16741 gttttttcat ttgactcaac gaggaatccc gatgattgct gcattatctg ctgttttttc
    16801 tttgcgtcag gacttgacta caaccgtttc ttttggaaag tctaggatca tcggcaatac
    16861 attaggtggt acattagcta tcatttattt ttttgtcaaa agctattttc acaatgattt
    16921 tttggtagaa ttgatcttgc ttcctattct agttattatt gtgatcgtcg tgtcagatgg
    16981 tattaacaat aattccggta tcatttccgc aatagctacg ttgttgctga ttgcgttgag
    17041 catccctcaa ggcgaatccg ctttgtatgc tatccaacgt gtactagata cttttatagg
    17101 gacattcatt gcaatcggta tcaatttctt tcttcgtcct cctgaaacag aaaagaagga
    17161 agaactatca gaagatttgg tcgaactcca aaagaaagaa cgattgctaa gaacaaattt
    17221 acaagagata gaagctaaga tccaaaaaca aaagaaaaat agcgaggact aattttcttt
    17281 tgcaataaaa taaaaaaatg gagagaagaa ctttaaaaag tcttcctcca tttttgattg
    17341 aacggtgaag tgtactactt tattcttcct tcatcgagtt ttgttctgcc tcttttggcg
    17401 cttcatacat atttacttgg ctagtagtcc cacgtgtcga caaattctct acgatttcct
    17461 tcaagtcaag tcctgttgtc gcttttagtg tttcttgagt agaagaaagc aaatttgtgg
    17521 cataatttgt cactcggttc gctccactgg tttctcctga actgcttcct gtatcgacaa
    17581 cagagatttt ttcgatattt cctagcggct gtgctgcttc gcgcatcaat tgcggtagca
    17641 tatcgataac catactcaag acagctgctt cgccatattc cttgaaggca ttggcgatct
    17701 tttgttttgc ttcggcttct gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc
    17761 cagctaaacg tgtcttattg gcttccgctt ctgccagtgc ttccacacgg aatcgttctg
    17821 cttcggcttc agtcacttct ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc
    17881 gatcggcatc tgcttttttc ttcacttccg aatcatattg cttttcacga cgtgtgattt
    17941 ctttttcttc cagttcgatt tgtttttgac gttctacgat tttaatctgc atttcctgtt
    18001 cgattacttg ttgttgtgct cgggcacttt ctaaattgta ggcttggtct gcttttgctt
    18061 tagcaacatc ttgttcttgt ttgtaggttg ctaatttcag ttctttttct tttaatgatt
    18121 ctgcgatctc tgtttgtcgc tgtaattcag ctgcttgtga ttctttttct gcttgagctt
    18181 ttttgatacg cgtttctttt agagcttccg cttctgctat atctgcatct cttttgactt
    18241 gggcgattct tggtttccct aaagagtcaa gataaccgtt tttgtctctg acctctttga
    18301 tagtgaaaga aacgatgatc aaccccattt ttgcaaggtc tacagaagcg acttcttgta
    18361 cgctttgact gaacttatcg cgattttgat agatttcttc tacagtcata gaaccaagaa
    18421 tcgagcgcaa gtgtccttct aatacttctc ttgcttcatt ttccagttct tctcttgtct
    18481 tgcctaaaaa ctgttctgct gctgtagcaa tctcttctac agatgaaccg attttgatga
    18541 tcgaagtacc atcacacatc accggtacgc cttgttccgt atacacttca ggggtggaga
    18601 cgtccaattt actcgataat agactgatcc gattcgaacg ttgaaaaact ggcaagacga
    18661 atgcgccacc tccacgcaca attttgattt tgttattgct ttcatcaatg tgcacatttt
    18721 tgcttcctaa ataacttcca ctgatgatca acgcttcatc cggttttgct gtctggtact
    18781 tcgctacaaa gacgatcagc aacatcagta aaatcaaagc tgcgattgcg atcgcgataa
    18841 aaacaactgg tatttccatt taacacacgc tcctttaagc ttttatcatg ctttcataag
    18901 gcatgacata acagattctt tccttgatct caatgatcaa tactttcgtt ccccgctcag
    18961 ctgtctgttc ttgggggctg taaaaacaag ctggacgact aatggaaccg gtaacacttt
    19021 tcaactgaat ttctcccata cctcgtacag ggatcggtgt gatcacaaca gctacttgtc
    19081 cttccagagt tttttcagaa gacgacatcg ttgattcggc atgtttcatc ggcatcaata
    19141 tatagaaatt aagcaaaaat accaacacta tcgatatgcc tccactagca ataaataaaa
    19201 ataaagatga ttgatctgtt aaacgctcac ctagaaaacc aaataagctt gtgaaagtta
    19261 accaagggat aataagcatc ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa
    19321 taatcaatag gactgccaat cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa
    19381 gtgcgattcc ccctatcata tacacgcctc cttccatttc tacgtctatc ataccaatgt
    19441 ttgaaaattc tgtatagctt cttttaccga ctcagtggtg ttttttatag gaaggttgtg
    19501 tttatcgttg acaatttgtt aaatttttgt tttcatgtta aaattttcac aataaattct
    19561 ttcgaggtag cctatgtttc ctttttttaa gaaaaaacca ttaaagctcc agaacacgat
    19621 catccttgtc gtttgtttct ccacattcgt ttctttgatt gtttctgcca tattgatcag
    19681 gaattttatt ctttcgatga accgatctag tgtgtcagat aaaatctcta gcattgctaa
    19741 aatcgttgcc aatgataaac tcatccaaaa aaagctgcag actggtgacc cgacacatgc
    19801 gattcaagga ataacaaatg aaatcatgat tgcatcgaat ttagatttta tcgttgtcat
    19861 ggaccctgaa ggcattcgat attctcacgc agacccaagt tacatcggtc ttcctttctc
    19921 aagtcatgaa gatgtgaaaa aagtgattgg aggacaagaa tttttctctg aaaaagaagg
    19981 aatgttaggg ttcggcttcc gctatttcgt ccctatttat tatgatcaag agattattgg
    20041 tgcagtttgt gttggcttaa caaacaacac gattgacaat caagtcaaag atggacaaaa
    20101 aatgattgtt ttttcattga ttattggact tagcgtaggc atcataggcg ccgtttttct
    20161 tgcccaaaaa atgaaaaaag tactgcttgg acttgaacca caagaaatag cagaacagtt
    20221 aagtgaaaaa acgattatcg aaaatgaagt agatgaagga atcttagcca tctcttctga
    20281 taaaactatc ctcttgatca atacacctgc aaaaaacatt ttctaccaaa cagagcagct
    20341 attggatttc gaagtaggaa aaaaatcagt gataatctat atgacgtttt gtttaaagag
    20401 acgattgagc atcaaacgac cgttcggact caatccatgt atttaaatgg gatagaaact
    20461 atttctagcg ttacacctct ttttagcaat gaccgttttt taggagcagt cgcaacttta
    20521 cgaaatcgat cagaagtaat gaaattaatg gaggagcttg gggggacgaa acaatatata
    20581 aactccatga gagctcaaac ccatgaattc atgaataaac ttcatgtcat ttctggtttg
    20641 attgatttga agaagtatga agaagtcacc cattttatcc agcagctaaa tcaaaattat
    20701 aaaaaagaga ttggccaagt ctcaagtctg atcaaagtac ctgcaattgc cggtttttta
    20761 ttaggcaaaa taaacgaagc caaagaacaa tctgttgaaa tgctgttaga aagcgattct
    20821 tttatcccta aaatggagat ggatgactcc atccattcac ttttgcaagt gttagggaac
    20881 ttacttgata atgcaaaaga agccgtgcta caaaaacatg aaagaaacgg acaggtccgg
    20941 attcatttaa actatgacac agaagctgaa gtattcatcg tgaccgtctg cgatgacggt
    21001 gtcgggatat cagaagaaat ctcagaaaaa atatttacgc taggtttttc gacgaaggga
    21061 atcaatcgtg ggtttggatt gaatctcatc cattcaatcg tcaaacgcca cggtggattt
    21121 ttagaatttt ccaaacaaac aaatggcggt acaatcgtct atctagaatt accaaaaaag
    21181 gaagatcaaa atgatgacaa atgtcctgat aatcgaagat gatccaatgg ttgcagaatt
    21241 gaatcgtact tttgtggaaa gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt
    21301 agaagaagga aaaaaatatt tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata
    21361 catgcctggc gaaagtggta tccatttttt atcttggctt agacagcaag gaaatgatat
    21421 cccagtgatt ttgatcactg ctgcagatga tatcgaaacc ataaagaatg tgatccgcta
    21481 cggtgcctta gattatttga tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga
    21541 aaagtatctt gctttctctc agacaatcca aacgactgaa aaagccgatc agaaattgat
    21601 cgatcaagta ttcaaccaca ccactcccat tcacgaaata ccatcattca tcgatttgcc
    21661 taaaggtctg tcaaaactga ctttaaaaaa aataagtgca tttattctaa aagaagatac
    21721 tattttttca acaaaagaac tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa
    21781 atatttgaat tttttagtag aacaagatat attgaacgaa agaatcaaat atttggaaat
    21841 cggccggccg ctaacctgtt atgaattgac aaagaatggg cggaaaaagc tcacagatta
    21901 ctgttgagtc atcgaattat cccatacaaa atagtccaag tatacagaag taacaactgt
    21961 ttacttggac tatttttaaa cattttattg acgaatatcc atgttttctt atcttaattg
    22021 acgggcttta ggaagagata cctcattttt cgtttttcca gtttctatta tttcacgaaa
    22081 gttctcgaaa cttgtcgaaa tcatatttga taatgcctca tctgtatttg aacctacatg
    22141 cggggttaca agaactctag ggtacatttc tactaattgc tggatagcag ggtctggaat
    22201 cgtctcccat ggcttgaagg atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt
    22261 tgcaaaacca gctaaatgat tgcttttcaa agctcttaaa atagcttcat tatcttgaag
    22321 ctctccacgg gctgtattaa tcaagatgct accttttttc atttgagcta aaaatccttc
    22381 attgatcatt tgatcgtttt cacctgggat atgaggtaca tgaatactga caatatcgct
    22441 tttggctaat aactcttcaa gcgagtggaa cgtcaatact tttttagcag cttctgtctg
    22501 atgaatatcg tagccgatca ctttggctcc tagtccctta aacaaaacag cttccgtcaa
    22561 ccctatcctg cctgttccta tgatccctac tgtgcaattt ctgatttcac gactaaacat
    22621 ttgctcatcc actcgaaaat ctttgaaaga tgttcggatc gtcgtatacg cagtatttct
    22681 caaaagcatc atggcaaaag tcaaagataa ctctgctatc gcatttggcg aatacgatgg
    22741 tacacgggct actgtcatac caaaatcagc tgccgcttgt agatcgatat gattgactcc
    22801 cacagtccgt gtaaaaacat aacgaatccc atattcctgc atttttgcaa gattggtctc
    22861 atctgctata caatttcccc tcaacaatac agcatcatgg tcctttgctg acacgatatt
    22921 gtcatgtgtc aacagttctt cataaagact gagatcataa tggtaaatat tcaatttttc
    22981 aaaaaaatct atctcgttcg gacgaacacc gtaacatgcg attctcatac tttcttcctc
    23041 catttcttat gaaaataaac ctatgccatg aatcatttgt aatacaacga tccatacagg
    23101 tgtcagtacc acagcaaaaa tagttgacag taaggatgca ttggaggcta aaagtgcctc
    23161 cctatcaaaa ctgatagcat aggctgctgc cactgttgca gctggtgtag ccatcatgat
    23221 aacgattgtt gccagtgctt caaaattgac tggtagaata ttcgttgccg tcaaaactgc
    23281 tagaatcacg atattcaatg caggtacaaa taccactttg actagactgt aataccatga
    23341 agttttttca gatgcagctt ttttgaagct tacttcacct aatgttgcgc cgattgctag
    23401 ccacgccaat ggtgaactta aaccagctaa ataatccatc ggctgaaata gccacggtgc
    23461 agtctggtca attcgcaaaa acgctacttg gcttatctct ccttctgcac ctgtaactga
    23521 cacttgtggc agataccctt ggaacagcca aatgaaaagg ccggcaaaag tagcgataat
    23581 gattggattg acgaacatcg ctttgatatt tttagcttcc atcttcaagc cactcatttt
    23641 tatgtatcca taagaataca aaaagattcg gtagccaata ttgaaaattg aggaatacat
    23701 cacgccgaca ggaccataaa tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc
    23761 aaagatcgtc aggacacgca acgttgtttg cttatcccct ttatagctta agaaaaaagg
    23821 ttttgaaact agaataagaa taatataaat aactaatccc caaatcaaga cattgattcc
    23881 ttgcatcagt aattctgtat tgatatcttg catgaaagct cgaaatgcca gtgctggcaa
    23941 agccactgtt aagacgactt tcgatagaat ttttccaaca gattcagaaa aaatcccctt
    24001 ttttctacaa acaaacccta ataatataat aaaaactgtt gaagtgattg cactgacaat
    24061 cgacatatct gtcaaagtcg atcgaaaaat gtcaaataag ttcatctttc tcctcctaat
    24121 tatgttcatt tattcacaat aaaaaaagct tttattttgc taatacgtat taacattttt
    24181 aattttatct ttacattgtt acttattgaa caataggaaa ttaaagaaac ttttgaaact
    24241 tatgtaaagt gattttt
//
LOCUS       DAYYCM010000040        23530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000040 DAYYCM010000000
VERSION     DAYYCM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.041"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..244)
                     /locus_tag="ACHAY1_001662"
     CDS             complement(20..244)
                     /locus_tag="ACHAY1_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593657.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(264..686)
                     /locus_tag="ACHAY1_001663"
     CDS             complement(264..686)
                     /locus_tag="ACHAY1_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8593658.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(828..1538)
                     /locus_tag="ACHAY1_001664"
     CDS             complement(828..1538)
                     /locus_tag="ACHAY1_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8593659.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1556..2053)
                     /locus_tag="ACHAY1_001665"
     CDS             complement(1556..2053)
                     /locus_tag="ACHAY1_001665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593660.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2090..2542)
                     /locus_tag="ACHAY1_001666"
     CDS             complement(2090..2542)
                     /locus_tag="ACHAY1_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8593661.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2677..3282)
                     /locus_tag="ACHAY1_001667"
     CDS             complement(2677..3282)
                     /locus_tag="ACHAY1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8593662.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3732..4352)
                     /locus_tag="ACHAY1_001668"
     CDS             complement(3732..4352)
                     /locus_tag="ACHAY1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8593663.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4354..5223)
                     /locus_tag="ACHAY1_001669"
     CDS             complement(4354..5223)
                     /locus_tag="ACHAY1_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8593664.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5246..5653)
                     /locus_tag="ACHAY1_001670"
     CDS             complement(5246..5653)
                     /locus_tag="ACHAY1_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8593665.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5672..6961)
                     /locus_tag="ACHAY1_001671"
     CDS             complement(5672..6961)
                     /locus_tag="ACHAY1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8593666.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            7019..8358
                     /locus_tag="ACHAY1_001672"
     CDS             join(7019..7460,7460..8358)
                     /locus_tag="ACHAY1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8593667.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(8400..9098)
                     /locus_tag="ACHAY1_001673"
                     /pseudo
     CDS             complement(8400..9098)
                     /locus_tag="ACHAY1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            9177..9353
                     /locus_tag="ACHAY1_001674"
                     /pseudo
     CDS             9177..9353
                     /locus_tag="ACHAY1_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(9529..10098)
                     /locus_tag="ACHAY1_001675"
     CDS             complement(9529..10098)
                     /locus_tag="ACHAY1_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8593668.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(10172..11461)
                     /locus_tag="ACHAY1_001676"
     CDS             complement(10172..11461)
                     /locus_tag="ACHAY1_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593669.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            11788..12663
                     /locus_tag="ACHAY1_001677"
     CDS             11788..12663
                     /locus_tag="ACHAY1_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8593670.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            12677..13000
                     /locus_tag="ACHAY1_001678"
     CDS             12677..13000
                     /locus_tag="ACHAY1_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593671.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            12997..13749
                     /locus_tag="ACHAY1_001679"
     CDS             12997..13749
                     /locus_tag="ACHAY1_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8593672.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            13739..15910
                     /locus_tag="ACHAY1_001680"
                     /pseudo
     CDS             13739..15910
                     /locus_tag="ACHAY1_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(16022..18193)
                     /gene="copB"
                     /locus_tag="ACHAY1_001681"
     CDS             complement(16022..18193)
                     /gene="copB"
                     /locus_tag="ACHAY1_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8593673.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(18213..20399)
                     /locus_tag="ACHAY1_001682"
     CDS             complement(18213..20399)
                     /locus_tag="ACHAY1_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8593674.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(20399..20608)
                     /gene="copZ"
                     /locus_tag="ACHAY1_001683"
     CDS             complement(20399..20608)
                     /gene="copZ"
                     /locus_tag="ACHAY1_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8593675.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(20621..21061)
                     /locus_tag="ACHAY1_001684"
     CDS             complement(20621..21061)
                     /locus_tag="ACHAY1_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8593676.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(21135..21633)
                     /locus_tag="ACHAY1_001685"
                     /pseudo
     CDS             complement(21135..21633)
                     /locus_tag="ACHAY1_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(21918..23384)
                     /locus_tag="ACHAY1_001686"
     CDS             complement(21918..23384)
                     /locus_tag="ACHAY1_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8593677.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         6782 a         4825 c         4096 g         7827 t
ORIGIN      
        1 ttcattctag tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt
       61 ctttgatgaa ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat
      121 cgaccatttt tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa
      181 gcttctcact atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat
      241 ccactgtttt tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa
      301 ttgccttcag ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct
      361 ggttcttcca tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc
      421 ggtggtccta atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat
      481 tctaattctt ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt
      541 tcagaaattt ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg
      601 acataaagca tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt
      661 ctttcctgtc tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat
      721 actattcaag aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt
      781 acaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct
      841 tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt
      901 ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat
      961 tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc
     1021 cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt
     1081 gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct
     1141 aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac
     1201 aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga
     1261 tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg
     1321 gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg
     1381 taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct
     1441 tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat
     1501 acttcccaga aattggtatc cactattact tttttcatct gttcacacct cttgattaga
     1561 ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt
     1621 cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc
     1681 aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta
     1741 gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt
     1801 ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac
     1861 ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt
     1921 ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat
     1981 tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat
     2041 ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc
     2101 ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta
     2161 aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg
     2221 agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga
     2281 gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta
     2341 atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt
     2401 cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta
     2461 aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga
     2521 tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac
     2581 ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact
     2641 agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt
     2701 gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc
     2761 ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc
     2821 accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt
     2881 ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc
     2941 ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc
     3001 aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt
     3061 gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc
     3121 ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg
     3181 gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga
     3241 aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt
     3301 agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata
     3361 taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac
     3421 ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac
     3481 caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca
     3541 gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca
     3601 caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg
     3661 aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag
     3721 ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg
     3781 taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg
     3841 tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct
     3901 ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga
     3961 cgatctgatt cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt
     4021 aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt
     4081 tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa
     4141 tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc
     4201 aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca
     4261 tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga
     4321 ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt taattggact
     4381 ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc
     4441 tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc
     4501 tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt
     4561 gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac
     4621 atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa
     4681 ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa
     4741 tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc
     4801 ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata
     4861 ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata
     4921 catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg
     4981 gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac
     5041 taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga
     5101 acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa
     5161 agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata
     5221 catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa
     5281 tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac
     5341 gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga
     5401 tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca
     5461 attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg
     5521 tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg
     5581 gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa
     5641 aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga
     5701 tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga
     5761 atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat
     5821 gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg
     5881 ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca
     5941 tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga
     6001 cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa
     6061 ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac
     6121 cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga
     6181 tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga
     6241 tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga
     6301 aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa
     6361 gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat
     6421 catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga
     6481 aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat
     6541 agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt
     6601 acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca
     6661 gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag
     6721 aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac
     6781 ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg
     6841 tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca
     6901 atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca
     6961 tgttttgatg tgcaccccaa aagttagact agaaatctaa ttaaaagggg tgcttttttt
     7021 gcggaaatat acatttgttt ttaagaaaaa agtagtttca gactatttaa ataacgaagg
     7081 cggctacaaa tatcttgcac ataaatatca aataaatcgt acactggtta gacattgggt
     7141 aaggatttat aactatcatg gttgggaagg cttggttgga ggtggcaaaa gctacactac
     7201 aaaatttaaa cttgatgtta tagaatatat ggaaacaaat ggtctttcta tccaagaaac
     7261 tgctaaaaaa tttaatatcg gttcaaatag aactctaagt aaatggatag agcaatatga
     7321 agaaggcggt gcttcttcac ttgagagcca aaaaaggggc agaaaaatta gtatgaattc
     7381 caagctaaac attcctaaaa aacttaaaga tgagtctctt gaagaagaag ttattcgttt
     7441 aagagcagag aacgcatatt taaaaaagtt agaaaccttg attcaagaac aagatttgag
     7501 caagaagaaa tcaaggttaa aacaatcctt cagctcaaaa aagaatttaa attgaatcta
     7561 ttactttcaa ttgcacaatt agcgaagtca acttattatt actgggtaaa aaaattagat
     7621 aagccagata aatatagcaa gataaagcaa gaaattacag cgattgtaaa agaatcaaga
     7681 aactcttatg gttatcgtag agtcacttta gcgttaaaga tgaaaggata tacaatcaat
     7741 cataaaacag ttagaaaatt aatgtcccaa atgggactta cctgtcaaat ccgaataaag
     7801 cgatataaat cttataaagg aacagtagga aaaattgcca agaatgtgtt aaaacgaaat
     7861 ttttcagtag atacacccaa taaaaaatgg gtgacagacg tcactgaatt caagataaaa
     7921 ggaagaaaaa tctatctatc tcctatactt gatttattta acggagaaat aattagttat
     7981 agtatatcaa ctagtccaac atataaactg attgaagaaa tgcttcaaca agcaattaaa
     8041 aaaaagggaa ctgagggttc actgattcta cattcagatc aaggatggca gtatcagatg
     8101 ccacaatatc aaaaaaaatt aaaagagaat aacattattc aaagcatgtc tagaaaagga
     8161 aattgcttag ataattctgt aatagaaaat ttttttggtg tgcttaaatc agagtttttt
     8221 tatcgagaaa aattccgatc aattgagata tttcaaagta aattaaatga gtatattagg
     8281 tggtataata acaaaaggat aaaactaaag ttaaatggac tatctccagt agaatatcgg
     8341 aaacagtcca ttaaatagtt caacttttgg ggttcagttc acaaagaggg ggggattttt
     8401 tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa aatcttgtgc tccaccctta
     8461 gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt atactttctt attagggaaa
     8521 tcacgcatat tgaaagatac atgatagcca acatttccta aagtattata agcttgattt
     8581 acgtctggtc tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag
     8641 tccataatga aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag
     8701 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
     8761 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
     8821 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
     8881 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
     8941 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
     9001 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
     9061 ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
     9121 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
     9181 cagctagaac gagacaagca ggaatacctt tagaagatat caaagatttt ttaggtcata
     9241 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
     9301 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
     9361 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
     9421 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
     9481 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
     9541 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
     9601 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
     9661 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
     9721 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     9781 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     9841 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     9901 cactgtttga cgaaggacat ctagttcttg caagagcgtt ttgtattgtt cttgggtcag
     9961 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
    10021 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
    10081 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
    10141 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
    10201 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
    10261 cgttatggtc tagtggaaaa aaatgatcgg tgatcactag atctgcatac cgaggcggct
    10321 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
    10381 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
    10441 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
    10501 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
    10561 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
    10621 ggtcatggat accaattacg taacgaagcc attcttggta aagtactttt atcagctttg
    10681 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
    10741 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
    10801 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
    10861 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
    10921 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
    10981 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag taaaacagca
    11041 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
    11101 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
    11161 aaggttcatt taatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
    11221 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
    11281 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
    11341 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
    11401 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
    11461 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgagt tcactcctaa
    11521 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
    11581 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
    11641 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
    11701 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
    11761 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
    11821 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
    11881 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
    11941 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
    12001 tttgaatttc gtccgggacc gatctttacc acgatcttgt tggcagatga gatcaatcgt
    12061 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
    12121 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
    12181 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
    12241 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
    12301 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
    12361 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
    12421 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
    12481 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
    12541 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
    12601 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
    12661 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
    12721 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
    12781 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
    12841 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
    12901 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
    12961 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
    13021 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
    13081 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
    13141 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
    13201 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
    13261 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
    13321 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
    13381 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
    13441 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
    13501 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac
    13561 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
    13621 tcagaagcta ttgaaacatt gtcaaaagat cgaacggctt atcttgttta tttcgtagaa
    13681 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
    13741 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
    13801 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
    13861 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
    13921 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
    13981 atggatctta cagttttttt cgtcagttac agaggaattc ttccatctat cccaaggtaa
    14041 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
    14101 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
    14161 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
    14221 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg
    14281 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
    14341 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
    14401 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
    14461 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
    14521 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
    14581 aaatcattac accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca
    14641 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
    14701 attgacccta aaaaaacaag ctttcttacc ttatccttat ggaaatacta ccttgactga
    14761 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
    14821 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
    14881 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
    14941 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
    15001 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
    15061 tttccgctat tccaaaacgg ataccgggca tactccgaaa gaacacgatt atgtggatta
    15121 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
    15181 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
    15241 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
    15301 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
    15361 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
    15421 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
    15481 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
    15541 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
    15601 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
    15661 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
    15721 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
    15781 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga
    15841 aataacgcgt ataataagaa aaacggtata caaaaaaatt ctgacaaaag ggagccgctt
    15901 caaaaaataa gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt
    15961 gagtatcgat tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt
    16021 attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca
    16081 agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcta
    16141 gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc attttctttc
    16201 gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata
    16261 cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag
    16321 cacgggctaa acttggcgca tcattgatcc catcacccac catgattact tttttccctt
    16381 ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt
    16441 cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca
    16501 tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta
    16561 ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg
    16621 tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct
    16681 caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga
    16741 ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat
    16801 aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt
    16861 gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt
    16921 ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct
    16981 ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggagcgag
    17041 caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta
    17101 ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga
    17161 tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta
    17221 gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat
    17281 tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa
    17341 tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt
    17401 caacaattgt ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga
    17461 cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt
    17521 tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg
    17581 cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag
    17641 ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg
    17701 aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag
    17761 gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa
    17821 atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg
    17881 gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata
    17941 gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt
    18001 gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc
    18061 ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat
    18121 gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat
    18181 cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagctc
    18241 agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga
    18301 ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa
    18361 agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat
    18421 gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt
    18481 cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct
    18541 gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct
    18601 ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg
    18661 gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc
    18721 ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca cccgatcact
    18781 tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt
    18841 tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa
    18901 tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt aaaatcaatc
    18961 atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga
    19021 tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga accgactaga
    19081 tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct
    19141 aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca
    19201 acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca tggacacgca
    19261 atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct
    19321 gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc
    19381 cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg
    19441 atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag cagaccattc
    19501 gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg aagattttca
    19561 ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga
    19621 atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac
    19681 gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt
    19741 ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc
    19801 agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat
    19861 ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata
    19921 tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa
    19981 aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc
    20041 gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc
    20101 gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt
    20161 ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc tacgctttgg
    20221 attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg
    20281 actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa
    20341 tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc
    20401 aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa
    20461 cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt
    20521 gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca
    20581 taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt
    20641 cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt
    20701 gtttcaaaat ctcattgata tcttcttgag tcaaagtagc ttcttctact agatccgcaa
    20761 tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa
    20821 ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttcctttt tgttctgtcc
    20881 agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct ggtttccaat
    20941 ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa
    21001 taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca
    21061 tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca gattacgctt
    21121 gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt
    21181 ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc
    21241 tgctgtattt ttggttcatt gtttccttca aaatgccaga taaaagataa aaattctaag
    21301 tatttcctgt ttagtttctc cgaaaatgat tcaggcatat ctggccgaga ttttttgttt
    21361 atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta
    21421 agccaaatga tcttatctgc ttctttaatc ctttcctcta acgtagaagt ataatttcct
    21481 tcaacgatcc atgacggatt agatactata aacgattgct gttcttgcaa aaaccacttt
    21541 tttgcctgaa tgcggaaagc cggtgatttc tttgatcttc cttgcgaggg acgattttcc
    21601 cgacccactt tgtccaatca cgatgatctt cattcttctc cctcacttct ctttggaatt
    21661 tggttcgatc ataccactta taacgccgca aaaaaacaag ggactgtgac aaaagtcgtg
    21721 tcaaagtccc ttgtttcgaa taaatggtgt tcaatcagct gaccttcagc attagctcaa
    21781 aaaacggcaa aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac
    21841 agctttttca caacctctga ttcacggtgt tccagaagta gcttctgtca caacctcttg
    21901 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtaat ttctttgttt
    21961 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac aattggtgtg
    22021 atgatattcg tgaataattg gaaccaccat gctttaggat cagcagtata atctactttt
    22081 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
    22141 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
    22201 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
    22261 aagttcgtca accattttac taattcattc atatctttga taccaaagat cggcaacaaa
    22321 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
    22381 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
    22441 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
    22501 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
    22561 tatgccccat ttgccatcaa gtctttaggg atattatcgc tagcaaacag catacccatt
    22621 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
    22681 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgcccct
    22741 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
    22801 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
    22861 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
    22921 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
    22981 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
    23041 ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc caaataagga
    23101 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
    23161 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc taattgtcca
    23221 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
    23281 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
    23341 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
    23401 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
    23461 gttattttat gtgtgtatta ttttatttaa tacacatcgt tttttcatat tttttccatc
    23521 aaaaaaataa
//
LOCUS       DAYYCM010000041        23434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000041 DAYYCM010000000
VERSION     DAYYCM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.042"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACHAY1_001687"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACHAY1_001687"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8593678.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACHAY1_001688"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACHAY1_001688"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8593679.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACHAY1_001689"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACHAY1_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8593680.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACHAY1_001690"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACHAY1_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8593681.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACHAY1_001691"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACHAY1_001691"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8593682.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACHAY1_001692"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACHAY1_001692"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8593683.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACHAY1_001693"
     CDS             complement(8297..8767)
                     /locus_tag="ACHAY1_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8593684.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACHAY1_001694"
     CDS             complement(8813..9238)
                     /locus_tag="ACHAY1_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8593685.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACHAY1_001695"
     CDS             complement(9305..9574)
                     /locus_tag="ACHAY1_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8593686.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACHAY1_001696"
     CDS             complement(9585..11180)
                     /locus_tag="ACHAY1_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8593687.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACHAY1_001697"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACHAY1_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8593688.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACHAY1_001698"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACHAY1_001698"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8593689.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACHAY1_001699"
     CDS             15646..16620
                     /locus_tag="ACHAY1_001699"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8593690.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACHAY1_001700"
     CDS             16790..18190
                     /locus_tag="ACHAY1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8593691.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACHAY1_001701"
     CDS             complement(18268..18990)
                     /locus_tag="ACHAY1_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593692.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACHAY1_001702"
     CDS             complement(19069..19575)
                     /locus_tag="ACHAY1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8593693.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACHAY1_001703"
     CDS             19698..21497
                     /locus_tag="ACHAY1_001703"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8593694.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21991..23379
                     /gene="ltrA"
                     /locus_tag="ACHAY1_001704"
     CDS             21991..23379
                     /gene="ltrA"
                     /locus_tag="ACHAY1_001704"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8593695.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6646 a         5056 c         4323 g         7409 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ttgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
    21601 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
    21661 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
    21721 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
    21781 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
    21841 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
    21901 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
    21961 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
    22021 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
    22081 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
    22141 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
    22201 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
    22261 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
    22321 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
    22381 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
    22441 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
    22501 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
    22561 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
    22621 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
    22681 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
    22741 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
    22801 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
    22861 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
    22921 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
    22981 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
    23041 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
    23101 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
    23161 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
    23221 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
    23281 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
    23341 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
    23401 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DAYYCM010000042        23412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000042 DAYYCM010000000
VERSION     DAYYCM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.043"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1708
                     /locus_tag="ACHAY1_001705"
     CDS             47..1708
                     /locus_tag="ACHAY1_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8593696.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1778..2140)
                     /locus_tag="ACHAY1_001706"
     CDS             complement(1778..2140)
                     /locus_tag="ACHAY1_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8593697.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2208..3149
                     /gene="opp3b"
                     /locus_tag="ACHAY1_001707"
     CDS             2208..3149
                     /gene="opp3b"
                     /locus_tag="ACHAY1_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8593698.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3150..4193
                     /locus_tag="ACHAY1_001708"
     CDS             3150..4193
                     /locus_tag="ACHAY1_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8593699.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4199..5269
                     /locus_tag="ACHAY1_001709"
     CDS             4199..5269
                     /locus_tag="ACHAY1_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593700.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5259..6212
                     /locus_tag="ACHAY1_001710"
     CDS             5259..6212
                     /locus_tag="ACHAY1_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593701.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6224..7234
                     /locus_tag="ACHAY1_001711"
     CDS             6224..7234
                     /locus_tag="ACHAY1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8593702.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7519..8340)
                     /locus_tag="ACHAY1_001712"
     CDS             complement(7519..8340)
                     /locus_tag="ACHAY1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8593703.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8437..8715)
                     /locus_tag="ACHAY1_001713"
     CDS             complement(8437..8715)
                     /locus_tag="ACHAY1_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593704.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8983..10992
                     /gene="metG"
                     /locus_tag="ACHAY1_001714"
     CDS             8983..10992
                     /gene="metG"
                     /locus_tag="ACHAY1_001714"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8593705.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11471..12058)
                     /locus_tag="ACHAY1_001715"
     CDS             complement(11471..12058)
                     /locus_tag="ACHAY1_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593706.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12233..13555
                     /locus_tag="ACHAY1_001716"
     CDS             12233..13555
                     /locus_tag="ACHAY1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8593707.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13710..14486
                     /locus_tag="ACHAY1_001717"
     CDS             13710..14486
                     /locus_tag="ACHAY1_001717"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8593708.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14479..15048
                     /gene="rnmV"
                     /locus_tag="ACHAY1_001718"
     CDS             14479..15048
                     /gene="rnmV"
                     /locus_tag="ACHAY1_001718"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8593709.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15041..15925
                     /gene="rsmA"
                     /locus_tag="ACHAY1_001719"
     CDS             15041..15925
                     /gene="rsmA"
                     /locus_tag="ACHAY1_001719"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8593710.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16376..17005
                     /locus_tag="ACHAY1_001720"
     CDS             16376..17005
                     /locus_tag="ACHAY1_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593711.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17129..18238)
                     /locus_tag="ACHAY1_001721"
     CDS             complement(17129..18238)
                     /locus_tag="ACHAY1_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593712.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18447..18869
                     /gene="mgsA"
                     /locus_tag="ACHAY1_001722"
     CDS             18447..18869
                     /gene="mgsA"
                     /locus_tag="ACHAY1_001722"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8593713.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19056..19154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19192..19728
                     /locus_tag="ACHAY1_001723"
     CDS             19192..19728
                     /locus_tag="ACHAY1_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8593714.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19768..21531)
                     /locus_tag="ACHAY1_001724"
     CDS             complement(19768..21531)
                     /locus_tag="ACHAY1_001724"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593715.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21534..23249)
                     /locus_tag="ACHAY1_001725"
     CDS             complement(21534..23249)
                     /locus_tag="ACHAY1_001725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593716.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7539 a         4288 c         4769 g         6816 t
ORIGIN      
        1 agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt
       61 tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc
      121 agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt
      181 agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt
      241 tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca
      301 gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa
      361 attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta
      421 tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc
      481 agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa
      541 agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata
      601 tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga
      661 tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt
      721 tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa
      781 agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt
      841 gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca
      901 aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt
      961 caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta
     1021 ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac
     1081 aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga
     1141 agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa
     1201 aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc
     1261 gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt
     1321 caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga
     1381 cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt
     1441 tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt
     1501 taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa
     1561 agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg
     1621 tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga
     1681 ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag
     1741 gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca
     1801 tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac
     1861 cgtagctggc agacgagagc gtggaaaatt ctggtttttt ctttcggttc cttgttcgtg
     1921 caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac
     1981 cgccgataat cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg
     2041 taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg
     2101 cgtaggggat agattttttt tcatttgatt gctcctttat ttttggttca ttatctaata
     2161 atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg aatagttata
     2221 tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta attgcaacaa
     2281 tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag gaacgtttga
     2341 gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca gttatcgtac
     2401 aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc ttccaattta
     2461 aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg cagttagggt
     2521 tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc tctgccatga
     2581 agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt cgttccattc
     2641 caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta caagttttgc
     2701 caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg ttggccatgt
     2761 cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta ctgcatagtg
     2821 attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc tttaaacatg
     2881 gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct gtagcattga
     2941 tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg gaacaattcg
     3001 ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc ctttactcat
     3061 ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt gatccaagaa
     3121 ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt tatgaaacag
     3181 ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa gaagaacgtg
     3241 aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt ttaaagaaaa
     3301 acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc tctgtgatca
     3361 caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc aatttgcctc
     3421 ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt ggtggtgaac
     3481 ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt ttaggaacag
     3541 acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc tcgttgctga
     3601 tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg ttggtttctg
     3661 gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc ttatcaggaa
     3721 ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga attttttcaa
     3781 tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt cgtgcacaaa
     3841 caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt gaatcaagaa
     3901 tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt attcaaatga
     3961 tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc ggtttaggtc
     4021 ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag acattcctgt
     4081 atctgcctta tctattatgg attccggcag taactttatc ggttatcatg attggcttta
     4141 acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag tgaagacgat
     4201 gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg ctggaaaggt
     4261 acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg cgatcgttgg
     4321 ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc tgtcaagcaa
     4381 cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg gaaaatcaga
     4441 aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag atccaatgac
     4501 ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta gaaagcataa
     4561 taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat tagttggaat
     4621 acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag gtcagcgtca
     4681 acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg ctgatgaacc
     4741 tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga aagaaattca
     4801 gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag tagcgaatgt
     4861 cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa catcggaaga
     4921 aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa tgccgacatt
     4981 agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt tattgaaccc
     5041 gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg atgcagaaca
     5101 agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc tacttgctcc
     5161 gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta tctttaaaga
     5221 gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa ctacttgaag
     5281 ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat gctgtaaatg
     5341 acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa tctggaagcg
     5401 gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat ggtgagatat
     5461 tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg aaattccggc
     5521 atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt atgaaagtca
     5581 gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct gaagagcgtg
     5641 caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac ggtactcgtt
     5701 atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga gcattggcag
     5761 taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg tcgatccaag
     5821 cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc tatctattta
     5881 ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta atgcataacg
     5941 gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt catccttata
     6001 cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga aaacgcagaa
     6061 gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt gaaatcgcac
     6121 cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa aaactaaaac
     6181 gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa aagcttttaa
     6241 attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt ttggatgtgc
     6301 tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac aaacaggagt
     6361 agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct taaaagaagt
     6421 agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc gcaattattt
     6481 caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc aatcttatac
     6541 gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt taccaaaaca
     6601 aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc gctccgctac
     6661 gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg tttcgccagt
     6721 aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga cattgatcca
     6781 aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag aaaagctgca
     6841 acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa agatcaggtt
     6901 agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt atctgaaaca
     6961 aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca aacaatatga
     7021 ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt ctattcacga
     7081 ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata aaaaatttct
     7141 tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc aagtagtaga
     7201 aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg ataggagcgt
     7261 tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa tttttctttt
     7321 gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa gttcgtacga
     7381 gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc aaaattctga
     7441 aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg attaggtctg
     7501 aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga tttttgaagg
     7561 acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca tagctttctt
     7621 aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa atacttttcg
     7681 agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct cgtgttccat
     7741 tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa aagccaaagt
     7801 atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt ctccgctaga
     7861 taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct tggcatcatc
     7921 tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga cgacccagcc
     7981 tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt cttttgaaat
     8041 aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg actcatcctt
     8101 ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt ttctttggat
     8161 caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga tatcgacagt
     8221 caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa gaaattcttc
     8281 ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa aacggttcat
     8341 ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga ctctgaaaaa
     8401 aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa taaatggaag
     8461 tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc gctcttcacg
     8521 aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca aaatccaagc
     8581 tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca tacgaatata
     8641 aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg taaaaaaaag
     8701 atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt tattatacca
     8761 cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa caaaaacccg
     8821 catttattca acctattcat ccttttaaag gatagattga acgtagatag aagtcgttta
     8881 tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg tatactagca
     8941 aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga aaaggaaact
     9001 ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg caactcgtat
     9061 actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt tgatgtgttt
     9121 tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga agaactaggt
     9181 gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt atggaaaacg
     9241 cttgatatca gttatgataa attcattcgc acgacagatg attaccataa agcagctgtt
     9301 caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga atatgaaggc
     9361 tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga agtgtacaaa
     9421 gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt agaattggta
     9481 aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt ggaatactat
     9541 gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat caacaacttt
     9601 atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg gggagtacca
     9661 gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc caattatatt
     9721 actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg gcctgcagat
     9781 gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc aatcatgctg
     9841 atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct attgatgaaa
     9901 gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt
     9961 tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg gtcagatggt
    10021 gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa tgatctaggg
    10081 aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact cgttcctgac
    10141 tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc taacgttgtc
    10201 ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc tgaaatctgg
    10261 gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt attagcaaaa
    10321 gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga atctttgcgt
    10381 atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat cttcaatcaa
    10441 ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga atttccttca
    10501 ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat ggaagaagaa
    10561 gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa
    10621 tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa atttgatgta
    10681 tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt agaaggggca
    10741 gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca aatcttgtca
    10801 ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta
    10861 gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat cctttctgca
    10921 gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc aaatggctca
    10981 gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc cctttttccg
    11041 aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat catataatac
    11101 agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt cgctgatttt
    11161 tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca taatgataaa
    11221 gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct tacattcata
    11281 aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag cgttgatgtg
    11341 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
    11401 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
    11461 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
    11521 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
    11581 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
    11641 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
    11701 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
    11761 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
    11821 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
    11881 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
    11941 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
    12001 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
    12061 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
    12121 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
    12181 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
    12241 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
    12301 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
    12361 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
    12421 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
    12481 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
    12541 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
    12601 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
    12661 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
    12721 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
    12781 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
    12841 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
    12901 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
    12961 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
    13021 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
    13081 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
    13141 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
    13201 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
    13261 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
    13321 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
    13381 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
    13441 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
    13501 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
    13561 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
    13621 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
    13681 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
    13741 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
    13801 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
    13861 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
    13921 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
    13981 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
    14041 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
    14101 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
    14161 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
    14221 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
    14281 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
    14341 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
    14401 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
    14461 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
    14521 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
    14581 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
    14641 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
    14701 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
    14761 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
    14821 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
    14881 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
    14941 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
    15001 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
    15061 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
    15121 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
    15181 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
    15241 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
    15301 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
    15361 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
    15421 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
    15481 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
    15541 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
    15601 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
    15661 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
    15721 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
    15781 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
    15841 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
    15901 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
    15961 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
    16021 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
    16081 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
    16141 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
    16201 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
    16261 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
    16321 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
    16381 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
    16441 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
    16501 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
    16561 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
    16621 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
    16681 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
    16741 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
    16801 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
    16861 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
    16921 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
    16981 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
    17041 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
    17101 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
    17161 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
    17221 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
    17281 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
    17341 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
    17401 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
    17461 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
    17521 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
    17581 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
    17641 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
    17701 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
    17761 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
    17821 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
    17881 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
    17941 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
    18001 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
    18061 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
    18121 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
    18181 aataattttc tgcattgtca tattttttat atacgttttt cagagccatt tctaccataa
    18241 tacgttccac actttctttt ttagttttat taactattta aagtataaat gaaagcgtta
    18301 actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat gtgctagttt
    18361 taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa ataagtatgt
    18421 taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa
    18481 aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa catgaacttt
    18541 ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg atccatcggt
    18601 ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg gaggatcagt
    18661 tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa ccagatgtaa
    18721 cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat atcggaacag
    18781 ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga atcgtccatg
    18841 aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat aacgtttaat
    18901 gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata taatacgaac
    18961 gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat gtgaaaatat
    19021 gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat aaagcgttaa
    19081 gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc ggttttattt
    19141 tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa
    19201 caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt gatggtcact
    19261 ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac atttattcca
    19321 caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc agttttagct
    19381 gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt tacattgaat
    19441 gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat cacttggaaa
    19501 aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt gacaccatta
    19561 tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc gccccgcctg
    19621 ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt aggacgtagt
    19681 attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa aacaagcagt
    19741 cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt gatagagccg
    19801 tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga caatagctcc
    19861 ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat gggcaatcac
    19921 aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt tctctgtcaa
    19981 tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac tgatcatcgt
    20041 tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt gaccgccaat
    20101 cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag aggcttttag
    20161 ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat tttctcgcaa
    20221 tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac gtaatgtatc
    20281 acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg ttgtgtcata
    20341 aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc ctactatagc
    20401 aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc
    20461 ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg gttccaaggc
    20521 ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa atgctcgttc
    20581 tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga taaatggttt
    20641 agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc
    20701 actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa ggtgatctac
    20761 aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc ctttttcata
    20821 tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct gaaaagcttt
    20881 cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc caataatcct
    20941 ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca caaggaaaat
    21001 aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca tcataaaaat
    21061 aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg cagaaattgc
    21121 aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt gggagttttg
    21181 atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc ttaattttgc
    21241 aacggataag taagctaacc gattgcttaa cacttggatg agccattgac tgactacgtt
    21301 gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga agcggacctg
    21361 tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca caactgttgc
    21421 aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat aggcagtaag
    21481 gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca taactattct
    21541 tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg
    21601 gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc ttcttgaata
    21661 gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg tagcgcctta
    21721 cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa aatcagtaat
    21781 gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa
    21841 tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg gacaaattct
    21901 tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc tttcttaccc
    21961 cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg aacaagagca
    22021 atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc cattataact
    22081 gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt
    22141 ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt aatattttgg
    22201 agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc gataattgct
    22261 tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct
    22321 agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac gacgaggttt
    22381 gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc aagaacttgt
    22441 ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa acagatgatc
    22501 ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt ggaaacacgt
    22561 aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc tttttttgtc
    22621 tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg ttcattgagc
    22681 tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat gataaaaaat
    22741 aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc ccagtcgatc
    22801 acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa taaacgaatg
    22861 accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt gatcaaggta
    22921 tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt gattagctga
    22981 ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg gcaaatgata
    23041 gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc aatgattttt
    23101 cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa aggcaggaca
    23161 agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg cttacgatag
    23221 tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat ttgtccacaa
    23281 ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa agagaacaag
    23341 ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata gactattttt
    23401 tttaaaaaaa ag
//
LOCUS       DAYYCM010000043        23159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000043 DAYYCM010000000
VERSION     DAYYCM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.044"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHAY1_001726"
     CDS             19..540
                     /locus_tag="ACHAY1_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8593717.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHAY1_001727"
     CDS             637..858
                     /locus_tag="ACHAY1_001727"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8593718.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHAY1_001728"
     CDS             complement(1015..1179)
                     /locus_tag="ACHAY1_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8593719.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHAY1_001729"
     CDS             1282..1803
                     /locus_tag="ACHAY1_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8593720.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHAY1_001730"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHAY1_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHAY1_001731"
     CDS             complement(2270..2428)
                     /locus_tag="ACHAY1_001731"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8593721.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHAY1_001732"
     CDS             2521..3282
                     /locus_tag="ACHAY1_001732"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8593722.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHAY1_001733"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHAY1_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHAY1_001734"
     CDS             4837..6057
                     /locus_tag="ACHAY1_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8593723.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHAY1_001735"
     CDS             6092..6769
                     /locus_tag="ACHAY1_001735"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8593724.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHAY1_001736"
     CDS             6766..7962
                     /locus_tag="ACHAY1_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8593725.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHAY1_001737"
     CDS             7977..8300
                     /locus_tag="ACHAY1_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8593726.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHAY1_001738"
     CDS             8304..8639
                     /locus_tag="ACHAY1_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8593727.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHAY1_001739"
     CDS             8636..9031
                     /locus_tag="ACHAY1_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8593728.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHAY1_001740"
     CDS             9028..9399
                     /locus_tag="ACHAY1_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593729.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHAY1_001741"
     CDS             9415..9975
                     /locus_tag="ACHAY1_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8593730.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHAY1_001742"
     CDS             10102..10413
                     /locus_tag="ACHAY1_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593731.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHAY1_001743"
     CDS             10437..10628
                     /locus_tag="ACHAY1_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593732.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHAY1_001744"
     CDS             10628..14473
                     /locus_tag="ACHAY1_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8593733.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHAY1_001745"
     CDS             14474..15346
                     /locus_tag="ACHAY1_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8593734.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHAY1_001746"
     CDS             15343..16392
                     /locus_tag="ACHAY1_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593735.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHAY1_001747"
     CDS             16396..17220
                     /locus_tag="ACHAY1_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593736.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACHAY1_001748"
     CDS             17238..19370
                     /locus_tag="ACHAY1_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8593737.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACHAY1_001749"
     CDS             19387..19536
                     /locus_tag="ACHAY1_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593738.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACHAY1_001750"
     CDS             19533..19979
                     /locus_tag="ACHAY1_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593739.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACHAY1_001751"
     CDS             19981..20118
                     /locus_tag="ACHAY1_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8593740.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACHAY1_001752"
     CDS             20155..20448
                     /locus_tag="ACHAY1_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593741.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACHAY1_001753"
     CDS             20471..20668
                     /locus_tag="ACHAY1_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8593742.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACHAY1_001754"
     CDS             20679..21698
                     /locus_tag="ACHAY1_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8593743.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACHAY1_001755"
     CDS             21955..22602
                     /locus_tag="ACHAY1_001755"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8593744.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACHAY1_001756"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACHAY1_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         8053 a         3925 c         5102 g         6079 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatatttta
//
LOCUS       DAYYCM010000044        22726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000044 DAYYCM010000000
VERSION     DAYYCM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.045"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHAY1_001757"
     CDS             complement(<1..210)
                     /locus_tag="ACHAY1_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593745.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(301..753)
                     /locus_tag="ACHAY1_001758"
     CDS             complement(301..753)
                     /locus_tag="ACHAY1_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8593746.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(766..1176)
                     /locus_tag="ACHAY1_001759"
     CDS             complement(766..1176)
                     /locus_tag="ACHAY1_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8593747.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1451..1663
                     /locus_tag="ACHAY1_001760"
     CDS             1451..1663
                     /locus_tag="ACHAY1_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8593748.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1666..1878
                     /locus_tag="ACHAY1_001761"
     CDS             1666..1878
                     /locus_tag="ACHAY1_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593749.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2018..2173
                     /locus_tag="ACHAY1_001762"
     CDS             2018..2173
                     /locus_tag="ACHAY1_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593750.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2170..2394)
                     /locus_tag="ACHAY1_001763"
     CDS             complement(2170..2394)
                     /locus_tag="ACHAY1_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8593751.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2472..2660
                     /locus_tag="ACHAY1_001764"
     CDS             2472..2660
                     /locus_tag="ACHAY1_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593752.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2799..2927
                     /locus_tag="ACHAY1_001765"
     CDS             2799..2927
                     /locus_tag="ACHAY1_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593753.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2986..3321
                     /locus_tag="ACHAY1_001766"
     CDS             2986..3321
                     /locus_tag="ACHAY1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593754.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3554..4495
                     /locus_tag="ACHAY1_001767"
     CDS             3554..4495
                     /locus_tag="ACHAY1_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8593755.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4497..5387
                     /locus_tag="ACHAY1_001768"
     CDS             4497..5387
                     /locus_tag="ACHAY1_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8593756.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5430..6230
                     /locus_tag="ACHAY1_001769"
     CDS             5430..6230
                     /locus_tag="ACHAY1_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8593757.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6245..7096
                     /locus_tag="ACHAY1_001770"
     CDS             6245..7096
                     /locus_tag="ACHAY1_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8593758.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7162..7296
                     /locus_tag="ACHAY1_001771"
     CDS             7162..7296
                     /locus_tag="ACHAY1_001771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593759.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7297..7758
                     /locus_tag="ACHAY1_001772"
     CDS             7297..7758
                     /locus_tag="ACHAY1_001772"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8593760.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7784..8236
                     /locus_tag="ACHAY1_001773"
     CDS             7784..8236
                     /locus_tag="ACHAY1_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593761.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8342..8536
                     /locus_tag="ACHAY1_001774"
     CDS             8342..8536
                     /locus_tag="ACHAY1_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593762.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8567..8953
                     /locus_tag="ACHAY1_001775"
     CDS             8567..8953
                     /locus_tag="ACHAY1_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593763.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8950..9153
                     /locus_tag="ACHAY1_001776"
     CDS             8950..9153
                     /locus_tag="ACHAY1_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8593764.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9150..9446
                     /locus_tag="ACHAY1_001777"
     CDS             9150..9446
                     /locus_tag="ACHAY1_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593765.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9522..9938
                     /locus_tag="ACHAY1_001778"
     CDS             9522..9938
                     /locus_tag="ACHAY1_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8593766.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10199..10336
                     /locus_tag="ACHAY1_001779"
     CDS             10199..10336
                     /locus_tag="ACHAY1_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593767.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10746..10976
                     /locus_tag="ACHAY1_001780"
     CDS             10746..10976
                     /locus_tag="ACHAY1_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593768.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11135..11968
                     /gene="terS"
                     /locus_tag="ACHAY1_001781"
     CDS             11135..11968
                     /gene="terS"
                     /locus_tag="ACHAY1_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8593769.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11961..13376
                     /locus_tag="ACHAY1_001782"
     CDS             11961..13376
                     /locus_tag="ACHAY1_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8593770.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13388..14917
                     /locus_tag="ACHAY1_001783"
     CDS             13388..14917
                     /locus_tag="ACHAY1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8593771.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14835..15887
                     /locus_tag="ACHAY1_001784"
     CDS             14835..15887
                     /locus_tag="ACHAY1_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8593772.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15889..16206
                     /locus_tag="ACHAY1_001785"
     CDS             15889..16206
                     /locus_tag="ACHAY1_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593773.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16333..16956
                     /locus_tag="ACHAY1_001786"
     CDS             16333..16956
                     /locus_tag="ACHAY1_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8593774.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16971..17861
                     /locus_tag="ACHAY1_001787"
     CDS             16971..17861
                     /locus_tag="ACHAY1_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8593775.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17884..18099
                     /locus_tag="ACHAY1_001788"
     CDS             17884..18099
                     /locus_tag="ACHAY1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593776.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18111..18452
                     /locus_tag="ACHAY1_001789"
     CDS             18111..18452
                     /locus_tag="ACHAY1_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593777.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18452..18823
                     /locus_tag="ACHAY1_001790"
     CDS             18452..18823
                     /locus_tag="ACHAY1_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593778.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18816..19211
                     /locus_tag="ACHAY1_001791"
     CDS             18816..19211
                     /locus_tag="ACHAY1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8593779.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19213..19590
                     /locus_tag="ACHAY1_001792"
     CDS             19213..19590
                     /locus_tag="ACHAY1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8593780.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19591..20199
                     /locus_tag="ACHAY1_001793"
     CDS             19591..20199
                     /locus_tag="ACHAY1_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8593781.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20199..20540
                     /locus_tag="ACHAY1_001794"
     CDS             20199..20540
                     /locus_tag="ACHAY1_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593782.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20782..22726
                     /locus_tag="ACHAY1_001795"
                     /pseudo
     CDS             20782..22726
                     /locus_tag="ACHAY1_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8210 a         3307 c         4675 g         6534 t
ORIGIN      
        1 attaatatca ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt
       61 acttaatata aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc
      121 taattcataa gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat
      181 taataaaata agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta
      241 ttgtagagtt tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt
      301 ttaatgttgt aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg
      361 gaactgactc atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc
      421 aaacatatta gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa
      481 agctgctgtg caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa
      541 tcttttcata gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg
      601 tatcgtttgt cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata
      661 tttgattatt ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat
      721 ttcttctaat atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt
      781 tttaggagtg aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc
      841 atcgtataat ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc
      901 atctgaattc aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt
      961 ttctgttaaa tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac
     1021 attaaagtaa tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct
     1081 agacatgctt ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg
     1141 ttcttttaat aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata
     1201 ccgacaaaaa aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat
     1261 actagttttg taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt
     1321 ctccccagac atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag
     1381 catatttgca gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg
     1441 ggtatgaagc atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag
     1501 tcaaagagag ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat
     1561 cgaaaaaaga aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt
     1621 agaagtagat tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg
     1681 agcaaagcgg agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg
     1741 aaggaattta aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata
     1801 acaattgatg tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga
     1861 gttagagctg tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt
     1921 gctgctctcc tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct
     1981 tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg
     2041 gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt
     2101 agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc
     2161 taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat
     2221 ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc
     2281 gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc
     2341 tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt
     2401 cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa
     2461 ggagaagtga tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac
     2521 aacgcaggaa acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag
     2581 ataggcaagc tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
     2641 atagggagat tagattatga agaagacaga cacacttttt ataggattca ttttggggtt
     2701 attagtgatt gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt
     2761 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     2821 aaatcggtaa agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca
     2881 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag
     2941 caaagagtca catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt
     3001 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
     3061 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
     3121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
     3181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
     3241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
     3301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
     3361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
     3421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
     3481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc accaattgat
     3541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga
     3601 ccgtaagaaa ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa
     3661 atctccttat caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag
     3721 tgagcccgct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
     3781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
     3841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc
     3901 taatcaattt ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca
     3961 agttcaacat tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg
     4021 tggtcaaaaa tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac
     4081 tgaacaatta gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga
     4141 tggtagtcaa gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca
     4201 aacgacgtta ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac
     4261 taagaaagaa attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg
     4321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg
     4381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     4441 agagatttat aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg
     4501 caacaaatga atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag
     4561 gtcaattagg acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg
     4621 tgcttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
     4681 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
     4741 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
     4801 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
     4861 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
     4921 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
     4981 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
     5041 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
     5101 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
     5161 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
     5221 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
     5281 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
     5341 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
     5401 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
     5461 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt
     5521 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
     5581 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
     5641 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
     5701 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
     5761 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
     5821 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
     5881 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
     5941 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
     6001 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
     6061 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
     6121 tgcgagaaaa atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa
     6181 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
     6241 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
     6301 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
     6361 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
     6421 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
     6481 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
     6541 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
     6601 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
     6661 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
     6721 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
     6781 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
     6841 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
     6901 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
     6961 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
     7021 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
     7081 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
     7141 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
     7201 aagataagta agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac
     7261 gtctacgaga atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt
     7321 tgcggtagta gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt
     7381 cgtcagcagt atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc
     7441 gcagactttc gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg
     7501 ccacacttaa gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac
     7561 gaactttggc cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct
     7621 tcaggaacat taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag
     7681 gctattggag aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt
     7741 atgaaaaaat tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa
     7801 taaatgtttt ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa
     7861 aaatattgaa gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac
     7921 gagatacaca ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc
     7981 gaaaatcgac aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga
     8041 tgattccagt ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga
     8101 aaaactagga agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga
     8161 tggggctgag tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca
     8221 aacaggtagt atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga
     8281 tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt
     8341 catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt
     8401 cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat
     8461 tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt
     8521 agtgacttgc gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca
     8581 ccaactcata tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg
     8641 gtttccaatt gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga
     8701 agatttaaga caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga
     8761 gtggttgaaa agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt
     8821 ttctgataaa aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt
     8881 ggcttgtaaa caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga
     8941 ggaagcggaa tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat
     9001 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     9061 gcaagtcagt ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta
     9121 aatgatgatc gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta
     9181 gtcatggtat cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga
     9241 aatcaaaatt caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt
     9301 ttcgtggata tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga
     9361 tggaatgcga taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg
     9421 atatggaact aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9661 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9721 cggagagaga cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac
     9781 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9841 gaggatacgg agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag
     9901 aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga
     9961 tagtgtaggg tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat
    10021 aggtctgaga caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct
    10081 tcgatagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
    10141 gttcttatta tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat
    10201 ggataaagaa atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa
    10261 caaggctagt gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa
    10321 aatccaagtt tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta
    10381 tcagaaaaat ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt
    10441 taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac
    10501 aaaataaaaa atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa
    10561 gacgcaaagg tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga
    10621 aaggactagt acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta
    10681 ggttgctatt acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct
    10741 acataatgag aaactactgg tatatatcgc taactaatga atatcctcga accattgatg
    10801 attgttcagt gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa
    10861 tgaccagaga agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg
    10921 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10981 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    11041 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    11101 caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    11161 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11221 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11281 cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11341 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa
    11401 gagaaataag aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac
    11461 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11521 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11581 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11641 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11701 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11761 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11821 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11881 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11941 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    12001 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    12061 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    12121 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12181 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12241 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12301 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12361 cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12421 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12481 ttgataataa gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt
    12541 cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12601 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12661 ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg
    12721 aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg
    12781 ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12841 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa
    12901 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12961 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    13021 ttatagaaga caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    13081 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    13141 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13201 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13261 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13321 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13381 tgagtttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13441 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc
    13501 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13561 caatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13621 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13681 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13741 aaatgatata gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga
    13801 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13861 agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13921 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13981 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    14041 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    14101 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    14161 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14221 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14281 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc
    14341 tactgcaaaa gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat
    14401 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14461 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14521 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14581 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14641 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14701 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14761 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14821 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14881 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14941 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    15001 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    15061 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    15121 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15181 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15241 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15301 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15361 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15421 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15481 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15541 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15601 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15661 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15721 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15781 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15841 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15901 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15961 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    16021 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    16081 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    16141 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16201 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16261 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16321 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16381 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16441 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16501 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16561 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16621 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16681 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16741 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16801 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16861 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16921 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16981 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    17041 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    17101 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    17161 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17221 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17281 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17341 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17401 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17461 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17521 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17581 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17641 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17701 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17761 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17821 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17881 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17941 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    18001 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    18061 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    18121 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18181 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18241 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18301 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18361 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18421 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18481 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18541 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18601 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18661 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18721 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18781 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18841 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18901 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18961 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    19021 gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    19081 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    19141 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19201 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19261 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19321 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19381 ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta
    19441 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19501 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19561 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19621 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19681 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19741 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19801 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19861 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19921 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19981 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    20041 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    20101 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    20161 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20221 ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg
    20281 aatttctacc aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg
    20341 ctgtagatgg aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata
    20401 ttttgtttga aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa
    20461 ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata
    20521 cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac
    20581 ttatcgagaa ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20641 tgaacgtact gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga
    20701 gcatgaggat gttgagtact acgataactt cacccaattt taggaggtga tactttgaat
    20761 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20821 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20881 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20941 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    21001 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    21061 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    21121 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21181 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21241 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21301 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21361 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21421 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21481 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21541 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21601 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21661 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21721 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21781 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21841 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21901 gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21961 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    22021 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    22081 acttggaatt ctgctaaata aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    22141 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22201 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22261 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22321 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22381 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22441 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22501 acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat
    22561 aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat
    22621 tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata
    22681 aatatttgga atgaagtggt aaatcaagct aaatcgattt ggttaa
//
LOCUS       DAYYCM010000045        22638 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000045 DAYYCM010000000
VERSION     DAYYCM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.046"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHAY1_001796"
     CDS             complement(227..1114)
                     /locus_tag="ACHAY1_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593783.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHAY1_001797"
     CDS             complement(1111..1980)
                     /locus_tag="ACHAY1_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593784.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHAY1_001798"
     CDS             complement(2117..3403)
                     /locus_tag="ACHAY1_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593785.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHAY1_001799"
     CDS             3676..4254
                     /locus_tag="ACHAY1_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8593786.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHAY1_001800"
     CDS             4254..5183
                     /locus_tag="ACHAY1_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593787.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHAY1_001801"
     CDS             5180..6916
                     /locus_tag="ACHAY1_001801"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8593788.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAY1_001802"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAY1_001802"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8593789.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHAY1_001803"
     CDS             complement(7812..8321)
                     /locus_tag="ACHAY1_001803"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8593790.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAY1_001804"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAY1_001804"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8593791.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHAY1_001805"
     CDS             complement(9622..10713)
                     /locus_tag="ACHAY1_001805"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8593792.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAY1_001806"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAY1_001806"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8593793.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAY1_001807"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAY1_001807"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8593794.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAY1_001808"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAY1_001808"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8593795.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHAY1_001809"
     CDS             complement(14676..15650)
                     /locus_tag="ACHAY1_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8593796.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHAY1_001810"
     CDS             complement(15631..17514)
                     /locus_tag="ACHAY1_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8593797.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHAY1_001811"
     CDS             complement(17532..19757)
                     /locus_tag="ACHAY1_001811"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8593798.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHAY1_001812"
     CDS             19872..20906
                     /locus_tag="ACHAY1_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593799.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHAY1_001813"
     CDS             complement(20973..21986)
                     /locus_tag="ACHAY1_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8593800.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHAY1_001814"
     CDS             complement(22064..22252)
                     /locus_tag="ACHAY1_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8593801.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6450 a         4863 c         4096 g         7229 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaa
//
LOCUS       DAYYCM010000046        22427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000046 DAYYCM010000000
VERSION     DAYYCM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.047"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..310
                     /locus_tag="ACHAY1_001815"
     CDS             11..310
                     /locus_tag="ACHAY1_001815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8593802.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            383..1744
                     /locus_tag="ACHAY1_001816"
     CDS             383..1744
                     /locus_tag="ACHAY1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8593803.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1766..2026
                     /locus_tag="ACHAY1_001817"
     CDS             1766..2026
                     /locus_tag="ACHAY1_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593804.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2040..2792
                     /locus_tag="ACHAY1_001818"
     CDS             2040..2792
                     /locus_tag="ACHAY1_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8593805.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2886..3491)
                     /locus_tag="ACHAY1_001819"
     CDS             complement(2886..3491)
                     /locus_tag="ACHAY1_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8593806.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3544..4518)
                     /locus_tag="ACHAY1_001820"
     CDS             complement(3544..4518)
                     /locus_tag="ACHAY1_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8593807.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4896..5615)
                     /locus_tag="ACHAY1_001821"
     CDS             complement(4896..5615)
                     /locus_tag="ACHAY1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8593808.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5742..7013)
                     /gene="celB"
                     /locus_tag="ACHAY1_001822"
     CDS             complement(5742..7013)
                     /gene="celB"
                     /locus_tag="ACHAY1_001822"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8593809.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7166..8563
                     /locus_tag="ACHAY1_001823"
     CDS             7166..8563
                     /locus_tag="ACHAY1_001823"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8593810.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8644..9084
                     /locus_tag="ACHAY1_001824"
     CDS             8644..9084
                     /locus_tag="ACHAY1_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8593811.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9232..9558
                     /locus_tag="ACHAY1_001825"
     CDS             9232..9558
                     /locus_tag="ACHAY1_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8593812.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9559..10308
                     /locus_tag="ACHAY1_001826"
     CDS             9559..10308
                     /locus_tag="ACHAY1_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8593813.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10388..10843)
                     /locus_tag="ACHAY1_001827"
     CDS             complement(10388..10843)
                     /locus_tag="ACHAY1_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8593814.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11270..11545)
                     /locus_tag="ACHAY1_001828"
     CDS             complement(11270..11545)
                     /locus_tag="ACHAY1_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593815.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11839..12654
                     /gene="thiD"
                     /locus_tag="ACHAY1_001829"
     CDS             11839..12654
                     /gene="thiD"
                     /locus_tag="ACHAY1_001829"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8593816.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12983..14434)
                     /gene="cls"
                     /locus_tag="ACHAY1_001830"
     CDS             complement(12983..14434)
                     /gene="cls"
                     /locus_tag="ACHAY1_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8593817.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14775..17441
                     /locus_tag="ACHAY1_001831"
     CDS             14775..17441
                     /locus_tag="ACHAY1_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8593818.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17805..18557
                     /locus_tag="ACHAY1_001832"
     CDS             17805..18557
                     /locus_tag="ACHAY1_001832"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8593819.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18579..19733)
                     /locus_tag="ACHAY1_001833"
     CDS             complement(18579..19733)
                     /locus_tag="ACHAY1_001833"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8593820.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19918..22359
                     /locus_tag="ACHAY1_001834"
     CDS             19918..22359
                     /locus_tag="ACHAY1_001834"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8593821.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7201 a         3933 c         4675 g         6618 t
ORIGIN      
        1 ggaagaaaaa atgaaaaatc ggttaattgt cgcatgtgga agtggagtag caacgagtca
       61 gacaatcgct agtaagatcc aaagtatgtt agaagatgac ggcatcgcat ttcctgtaga
      121 agcagttgat tacaaatcga ttcaaaatga gcttctaact gctggaatct atgtgtatgt
      181 cgcacaacct gatgaggaag tacttgaaca agcaaaagat ttgggtattg aagtatttcc
      241 aggaattcca tttttaactg gaatgggtgt agaacctatt tatgacagta tcaaagagtt
      301 agtacagtaa aggaggattg ttggataaaa aagttggttc atagttttat ttactatatt
      361 tagaaagaaa gaaggtttat ttatggaagt tttccaaacc attatcaatt atgttttgaa
      421 cttaggatca gccatttttg ttcccctgat tattttgata cttggattgt tggcagggat
      481 gaaattaaaa aaagcattca tgtctgcttt gactttaggg attgctttta gcggaatgtc
      541 tatggtcatt ggctatatgt ctaacgcagt aagtccagca tcagaagcat tggctaaaaa
      601 tacgggaatc agtttgcctg cacttgatct tgggtggaca ggtgcagcaa gcatcacatg
      661 gtcgtggagt tatgctttta tattttttgc agtaacgatt ggggtcaatt ttgtactgtt
      721 actttttaat tggacgaaga cgttaaatgt tgatatgtgg aatgtttggg ggaaagcttt
      781 aactgcttat ttagtttatt atgtttccgg tagtttggct gcaggctttc ttactgcaat
      841 ggtccaagtt atattggaat taaagcttgg tgatatgttt caaaaacata tacaagatct
      901 cacagggatt ccattagtaa cagtgactca ttttatgaca tctgccgctg tcttgttgct
      961 gcctttcaat atgatcatgg ataaaattcc tgctttaaat aaacgagcgg atacgaatgc
     1021 attgaagaag aaaattggga tcttctctga aaactctgta atgggattca ttattggatt
     1081 ggggcttggc tttgctgctg cgtatggcgt ttcaggttct cttaatctag cgatccaaac
     1141 agctacagca ttaagtttgt ttcctatgat ctctaaaatg ttcatgcaat ctttgtctcc
     1201 tctagcagac gctatgtcag aatttatgaa aaagcggttt aaggatcgtg aagtatatat
     1261 cggattggac tggccgatat tagctggtcg ttcagaaatt tgggttactg ctattttact
     1321 tgttcctgtg tttattggtt atgccattat tttgccagga aaccaagtat tgccattagc
     1381 agggattatc aactattcga ttgcggtcgg cggattactt ttaacaggag gaaacttggc
     1441 tcgtatgctg atcttgggaa tcatcactat gcctatttat ttgtacgggg ccacttacct
     1501 tgcgccaata ctcagtgatt tagcagaaaa aagcggagca gtcgagggaa tcaagaaagg
     1561 acagttgatc acatggtcat ccatagaagg gccggagttc agggttttat ttgcggaggc
     1621 atttaatagg aatattctcg ctattgtagg atgcgtgatt tttctaggac tgtttgttct
     1681 actttataaa tatatgataa aagcgaaagt accgagtgaa cgttatgctg aactacaaaa
     1741 atagcattga aatggtggga aagtaatgaa aagagaagag acagataaaa taaaatggac
     1801 agtcgctctt tgtggtacac ttctgctatt tctatatgga ctctttactc agaatatcat
     1861 catcaatcta ttggtcattt ttttcgcact agtgatctat aaatatggaa atcacgtttt
     1921 attcagagag tacgatgaaa aaagaaaacg gaaaatagaa gaatcaatga aaataaaaga
     1981 agctaccaaa gaaatattga gagaaaaaag ttttataaaa agataatgga ggggtcatta
     2041 tgtcagatgg acgtatgtta tttgagagag aacgacagga catggcacgt atcgtaagag
     2101 tgattttcga aaggaaaaac acaaatgtcg caggagggaa tttttccttt aaaaccactg
     2161 atgatcaagg gaaagactac atcatcatga cgccgaccat gatgtcagaa gcctatctag
     2221 gagagatttc tcctgcacag gttctagtag tagaaccaag aacgcgtaga atcgttgacg
     2281 gagtcggtca tttaactcgt gaaatcaata tgcatgaagc gatgtatgag actaatccag
     2341 ctatcaaagc tgtcctacat gctcatgcgc ctaatgcaat gttctgggcg actactgggt
     2401 tgcctatgcc taatttgaca gaagccacag ctaaagtaaa agagattcct gtcatggact
     2461 ttgagcctaa ttgctctgaa gaacttgctg aattgacaag cgactatgta aaaaaacagc
     2521 atctcgaatt accgcatatg ttgctgctca actctcatgg cgtattgatc aacacaggtg
     2581 gagaatcagg aatcgaagct atccataaag cactgcaaat ccttgataca gttgaatgga
     2641 atgcagaaat tgcctacaag cagacgattt ttcaagcatt agggattact gacggttttt
     2701 attcaaaagg taagaaaatc gggggattgg aagagttgaa acataaaacg gctatttata
     2761 ataaatctgt tattgcagct ggtggagact aacctatctg acacaaaaaa ctacgacttt
     2821 cacaaaatct gatccagatc gatatctgct tcaggttgta ttatgtaagt cgtagttctt
     2881 ttcttttatt tagttaagag gtggtctgtc cattccctcg cttgcttgat ttcgtccatc
     2941 gtcaactgat gacctgttac agttagctga atagtcacat ttgcttggcg tgtttcgaat
     3001 tgatcggcca attctttaaa ggaagcaggg gaaacgattg gatcgttatc accataagaa
     3061 atccagacgg ttttatcctc taatgtggaa cggctttgat gaacacctag agacatcgga
     3121 tggaaaaaca accctttatt tagctcggtc tcacgctcca ataataaatg agcagcgatg
     3181 tttgcgccat ttgaatagcc gacaagcagc cagtcactta acggtatcag ttctttttcg
     3241 ctgatttctt tgattttttc atggagttta tctgtttctt tttccaaact gtccaggtcg
     3301 aattgattca atccattacg cttgaaaaaa cgattcatcc catcttcttg gaccgtgcca
     3361 cgaaaagaaa gcaatgtact gtttggtgca agaaagtgag cgatatccag taaagaatgt
     3421 tcatcgccac ctgtgccatg aagtaagatc aaatgcttgc cacttgttcc tttttcaaaa
     3481 atgtaggtca ttttgttccc tcttttctgt ttctatagat gcaaatgaag tagcatgaag
     3541 agattattca cgttttttat ttgcatcact tgtatcgaaa ggacggacat aagcttcaat
     3601 ggcttcacga cgttcttcta agaatggagg aagagatagt ttttctcctg ctgattctgc
     3661 tggttcatct tcaaggaatc caggaccatc agttgctaat tcaaacagta cgtgatgagc
     3721 ggctaggaag tactcagatt ggaaatagaa acgatcgacg aaaccagaat ttggcaattc
     3781 aatcttattg attacttgaa tccagtattc caatgcttct ttgtctgcta cacgcaatgc
     3841 taagtgatgg atatttccaa atccttcgat tgcttttggc aagtctgtcc gatgttcaat
     3901 aatgatgctt gcaccatttc ccccttcacc gatttcaaat aagtagaaat ctcctgaatg
     3961 gtctgtttct ttgaatccta agattttttc aagaatcaag cggatatgct ctaattgact
     4021 gacacggaca aagacaggtc caagtcctgt gatcgcaaac tctgtcggta cattgctgtt
     4081 tttccaagga atgccagctg ctacaccttg attgttttcg tcagagatca attgataata
     4141 ctgattatcg aaatcttcaa attcgatata ttttttgccg aaacgttcat tgattgctga
     4201 atgagtaacg tcatattttt caaaacgttc caaccaaaag tctaatgctg cgtcacttgg
     4261 cacacggaaa gaagcacgag agatcgtatc tgttcctttt tttccttttg gctgatttgg
     4321 aaaatcaaag aaagtcatat ctgttcctgg cgcaccagta tcgtctgtaa agtaaagatg
     4381 gtaagtttcg atgtcatctt ggttaactgt ttttttgatt aagcgcattc ctaaaatatc
     4441 tgtaaaaaag cggtagattt ttgtagcgct agatgtcatt gctgtgacgt ggtgaattcc
     4501 tctgatttgt gtatccatga taaatttcct cctgttaact tacaaaaagt aagtagttga
     4561 tttagacata gtatagaggg tgcatctcta ttttgcaatt aatttgtctt ctaaagtatc
     4621 aggaaggaat caagaatgga aaagaggagg gacggatcaa taaaataaaa agacttacga
     4681 ctagctgtat cgatttcaaa aaaactagct ttaaaaagct aactttagaa tcattacggg
     4741 tggtcataag tctttttatg ctgagttatt ttttcatttt tgagctaatg cttaggatag
     4801 aatagaatgt tgatttcacg atctcttctt gttacttgaa actgcttacc actgtcacga
     4861 tctcttggta aacggctttt tcacaacctc tttggttaag ttgaacgcac atccagcaca
     4921 gaatagttcc gaacatcaaa tcggtttcgg ctggaagaat attcaatcgg ctgtccatct
     4981 ttgagataga cgacttgctg aacttccagt acaggatcgt tttctgcaca gttcaaatat
     5041 tgctgatcat attcatcact tttatctgca gaaatagcac gataagcccc tgcaaaatgg
     5101 acattcaatt cttgatgaag atgatcatag atagaggcta acaaatgttc tttttttaaa
     5161 cccggcacaa gatggactgg cataaaggta tgctctaaaa catatggctc gccatctaag
     5221 atccttaacc ggtgaatatt gtagacaggt tgctcggcat cgatcatcaa tcgttcttgg
     5281 atatattgat tagggaattc aacatcaaaa tgaatgattt gactcttcaa agaacgatgt
     5341 ctgcgtttca tttggagact caaaccttca tactcgttag cgggcgatgt gtctttttgc
     5401 ataaatggat gattcaagat tttcgttcct gatcctcgct gcgaatagac aagtccttcc
     5461 attgctaaga tattgatggc ttttttgatt gtcatccggc tcgcaccaaa ttcttttacc
     5521 aagtcgactt gattaggcaa aaaactatcg agaggatact cttttgaacg gatacgttct
     5581 cttaaaatat ttgcgatttc ttcgtatttt ggcacgttat tcacctcttc tagagtataa
     5641 cgaaagatta tgcttccgac aactcgaagt agcatctgga acattgttta ttcgaaaaaa
     5701 gaggtcatga caagtttttg tcacaacccc ttaatgatct attaagcaat atctaaatct
     5761 ggttgtttat ctagtacttt caggaatggg taccagataa aggcaacgat gacaaagtcg
     5821 atgacttgca gcataccacc aagccatgaa tttgttgcta agacaccgct tgcaatgatt
     5881 ggcacagtcc atggaacaga gacgccagtt ggtgcaggga caagtccagc agccattacc
     5941 aaataattga acgtcgttac gattagaggt gccacaaccc aaggaataag gattgttgca
     6001 ttcaatacga tcggtagacc gaagatcgct ggttcattta cattgaaaat accaggtgct
     6061 aaagctaatc gtccaacatc acgatactgt ttctttttaa gaacaaaggc catcaataaa
     6121 acgacagcta aagtcattcc agagccacca ataccaacag tgaaggtttc catgaatggt
     6181 tttgtgatga tgtgtggtaa ttcttttcca tgttgataag cttctaagtt atcaagcatc
     6241 agtgtattcc aaatcgggtc cattacagag ttgacgatga tttgtccgtg aagtccgaag
     6301 aaccataaga attggacaaa gaacaaagca atcaatgttg caggtaaact acttcccaaa
     6361 ccagttaatg gtttttggat cacttcataa acgacgtcat gtaagttagc attgaaaata
     6421 ccagtcatca cagcattgat tactaagaaa atagttagtg tcactacagc agggatcaaa
     6481 gcggcaaatg attttgttac tgcaggcgga actccatcag gcattttgat ctgccagccg
     6541 cgtttcgtga ttctgcagta gatttctcca gccaagaaag cagcgatcat tccaatgaac
     6601 attccttttg cgccaagacg atcaagtgaa agcacaccag tcacatcttc accgccagcg
     6661 gttttcattg caaaaggcgt aagaatcaag aagctggcaa aagacacggc accaccgaaa
     6721 ataccttcaa cgtcataaga ttttgataaa taatagccga taccaaaagt aacaaaaacc
     6781 gacatgatgc tcattgttgc attttgccca ttaccgaata aattcgataa agttcccttt
     6841 gttgcgtcac tgaaaaaagg caagttattg ataacgacaa cgatcgaacc gaacatcgtt
     6901 aaagggaagg aaagcatgaa agcatcccgc agcactgtaa ggtaacgatt ttgccctaat
     6961 aaattagcta agggcatgat tttctcagaa agcttgtcca taaaaccgtt catttttatt
     7021 cacctcaaat ttatttgcaa tcgattacat acaaagtata acgccaaaaa ttttaaatgt
     7081 ctagtcattt tcataaaaag tattgattta ttttttaggg ttcgttatga ttacggtatc
     7141 aagaaaaaag agaggaagta aaaaaatgaa aacctacaca ttcccagaaa atttttggtg
     7201 gggttctgct gctagcggac cgcaaacaga aggaagagta agtgatgatg gtaaaggaga
     7261 aaatatttgg gatcattggt atgaattaga gcctgaaaaa ttcttcgatc aagtaggacc
     7321 tgaaaaaact tcgcaagttt acacaaaata caaagaagat gttcagttaa tgaaagaaac
     7381 tggccataac acatttcgta catctatcca atggagtcat ttgattccag aagggacagg
     7441 tgaaatcaat caaaaagctg tttcttttta tcgagattat tttactgaat tgctggataa
     7501 tggtattgaa ccttttgtga acctttacca tttcgatatg ccactaccac tgcaagaaaa
     7561 aggcggatgg ttatcaagag aaacggtcga cgcttatgtt tcttatgcag cagcgtgctt
     7621 tgaactgttt ggagatctgg tcaaaaaatg gtttactcat aatgaaccaa tcgtacctgt
     7681 agaaggaggc tacttgtatc aattccacta tccaaatcat atcaatatga aagaagcggt
     7741 ccaagtaggc tatcatgaga atctagcaag cgcaaaggta atcaaagcct accatgaagg
     7801 cggacatgat ggtgagatcg gaatcatctt gaatctgaca ccaagctatc cgagagatga
     7861 aacgaaccca gaagatgtaa aagcggcgaa gatcgctgac gcattcttca accgatcatt
     7921 ccttgaccct gcagtgaaag gagagtttcc acaagagttg atcgaaattg tcaaagaatt
     7981 ggctataatg ccagagatcc atgaaggaga cctagaactc atccgtgaaa acacgattga
     8041 cctgctagga atcaattact atcagcctcg tcgaatcaaa gcaaaagaaa ctccaatcaa
     8101 tcacgaacat agccccatgc cagatgatta ttttgataat tacgacatgc ccaataaaaa
     8161 aatgaatcca tatcgtggat gggaaattta cgaaaaagga atctatgata ttttaacgaa
     8221 tacaagagaa aactatggta atatcaaatg ctatatttct gaaaatggga tgggggtcga
     8281 aggagaagaa cgatttgtta acagcgaagg aattattgaa gatgattacc ggatcgagtt
     8341 tgttacggat catttgaaat acgtccatca agcaatccaa gaaggaacca attgtgtggg
     8401 ttaccacatg tggacatgta tggacaactg gtcttggaca aatgcttata agaatcgcta
     8461 tggctttatc gctgttgatt tggcacaaga cggtaaacgg acgatcaaaa aatcaggtcg
     8521 ctggtttaaa acggtctctg aaaaaaacgg atttgatgcc tgatattttt tgataaaacg
     8581 ttcacaaaat gtttgttcta acactgtaat cgtgctacac taacagtgaa ggaggcgttt
     8641 acaatggtag atacttatcg taatatttta gtagcaattg atggttctga aaaggcagaa
     8701 aaagcatttc ttgaggcaat cacgattgcc aaacgaaatc aggcaacgtt gcacatcctg
     8761 tatgtcaatg aagtaacagg caattacttt ggtgattttg catttgttac aacaagcctt
     8821 caggaagaat tggatgaggt tgcggaaaac cagatgaaag aacatcgaaa cttggcaatt
     8881 gaaaaaggcc tgacagatat cgagacatat gttctttacg gttatccaaa gacactgatt
     8941 gctaatttcc atgaatcgaa agaaccaatc gatctgattg tcatgggggc gacaggtctg
     9001 aatgcggtag aacgtgcact agtaggttcc actacttcct atgtagtcaa ccacgcatca
     9061 tgtaacgtca tggttgtaaa ataaataaat taagggaaca gaaagcaaaa aagcataacg
     9121 cttcttttgc tttctgttcc ttttattttt ttgtaaaaaa ttactttacc tgatgaagta
     9181 atcatgctat tctaattaca acgacagatg gagtaatatg gaggaaagaa aatgatttca
     9241 agtgatggaa tacgcggata caatgatgcg attattttat cgatccttaa caaaaacgat
     9301 tcttatggat atgaaatttc taaaacgatt cgtgagagga caaatgattc ctatgtgatc
     9361 aaagagacaa cactttactc cgtttttgcc cgtttagaaa agaatcaact gattgtttcg
     9421 tatcctggag aagtgactca tggaaaaaag cggacatatt ataaaatcac tgaaataggg
     9481 cgtaatcttt tagaagaaaa aaagcaagag tggctgacga cgaaagacgt agtcgatcaa
     9541 tttttggagg gaaattaaat ggatacaatc cgtaattatt tagacagtct ttttatcgga
     9601 gtacctcaaa gtaccgagat agataagctg aaaactgatt tgttggcaaa tatggaagac
     9661 cattatcatg aattgatggg agaaggaaaa aatgaacagg aggccatcgg tacagtaatt
     9721 tcgacatttg gttcaatcga tgaattatta gaagaactag atgtcgaaaa aaaacatcaa
     9781 gctgacgaga cggaaacaaa cacagcttct atttatttaa gcgaagcaga aaactactgg
     9841 aaagaataca gagcggcaag tctccaagtg gcatcaggtg ttctattcat tagcttatct
     9901 tttgcaagtt ttcttttctt ttgttcagca ggatatgtgt tcatgggtat ttcttgtctg
     9961 attttcggta tagcattagc tgtcggtttt tttattgctt ctggtatgaa aatcacacgt
    10021 ttaaatcact ttttgcacca tcggaaaata cctgaaaaag tattagcaga agcaaaagaa
    10081 aaagaagaag agtatcagcg aagttttggc ttctcactga ttgcaggaat tggattatgt
    10141 attttttcac ttttcccact tttggcttct ttaatgtggt atatggatgg tagtatcggt
    10201 gcctctatat tttttgttac ggtaggaacg ggtgtcttct tgatcattta tggcagtcta
    10261 gtccgtcatt cttatcgtca attcactcaa agcgcttatt actggtgaat gtattcagat
    10321 ttgtaaaaat attctattta tccggcgtag aaaaaaagac tgtgacacgc ttttgtcaca
    10381 gtctttttta tatataataa tttaaaaatt catcatctaa atccgtagaa ttttctagat
    10441 tgcttaccca tgtaaaatcg ttatagacag atttcagttg acttctgcgc cattgtacag
    10501 ggataaaaag acgcatgcta tcttcattga atccgactaa taatttcttt ccgtcagtaa
    10561 tgatgggacg ttgtaacaaa gtaaaatgcg agacgatcag atcgacaagc tctgaggtcg
    10621 tacattggtc gatttggtct ttcaaatttt tataatcaat cgagcgttta gctaatagat
    10681 cgtcaaatcc gttttctgtt aatgacaata aataaaatat ctctttcctt gttaaaggct
    10741 gtttaggtgt gaaaattcgt tctctgtaag gcatttgatg gtctttcagc cagtttgcca
    10801 tcagatggta actatgtgtg cgagaagaat agatcgtaag cataaaaaaa ctccttaaat
    10861 agtccattaa tttgttagat ctgttgagat aaataatagg taaggagctg tgcataagca
    10921 ttgatcaaaa caacccaaac atcattagaa ataactttct aaagtcttaa acttgcgacg
    10981 agaagcaact agactaatag aaaaaatgtc ttagccattt ggcttcctgt ttgttttttt
    11041 gcttatgctg cagctcctcg ggaaagggtt ctaacaattt gaagaaggtt gatgattgat
    11101 ctagtttaaa ttgtgtgaaa tttgaaatct ggtgttttta ctagatgaat cacctaacaa
    11161 ctatgattat aacttataga acttctgttt tgcattcggt tttgttatag ttgttataaa
    11221 gttttatttt attacagaac atttttaatt aataaagaac agttagaagt taaaattgct
    11281 gattcacata gagtttatga cttcttggaa attctttttc aatcagtttt ttcatctgtt
    11341 tatgaaattc ttttttttca gtaaacataa aggtttgggt atcgtaacct tcttcatgta
    11401 ggaaatagat tattcctttt tctgtgtttt gcgtataagc ttttatactc ctgtcttttt
    11461 tgaaagaaca gatcgtatag ccatctattt ggtctggtag tcgtttgatt ttcttcaaaa
    11521 tgactttttt atcctcaagt ttcatttttt tcacctgact ttattttttt catatggaaa
    11581 tcatagcgcc tttggttata tgaaaacaag aaggatactg aaatatatat aaatcaaata
    11641 gtggttattt attattagaa tagcgaatac aaataaacga ccactatttt ctgatttatt
    11701 gcaaatgttt tttcaaataa atttgattac caagaaaagg tagtggtata tttactgagg
    11761 gatactttga aaaacatatg atagaaacag taacagtatt atgaaaaatc tttttataaa
    11821 aatggaggaa tcgatttgat ggaagatcag tatgtacaag caatgacgat cgcaggatca
    11881 gatagcgatg gaagcgcagg gatgcaagca gatatgcata cattctttac aagaaatgtt
    11941 tacggtgttt ctgtcatgac cgcatgtgtt gcaggtaatt cttacggaat cggagcaagt
    12001 gtgacattgc caacagattt tatcgacaaa gaatttgaac tgattgcgaa ggactttcaa
    12061 atacgtgcag ctaaaacagg tatgctagca gattcaaagt tgattgagac agttgtaaaa
    12121 aattaccaaa aatatgattt tggaccatta gtcgtggatc ccgtaatcgt aacgaaacat
    12181 ggaaatcttt tactagaaga atctgctttg caaagcttga aagaaaagct tgtccctttg
    12241 gccgaagtac tgacacctaa tttttatgaa gcagaaaagc tgactggacg gacattagca
    12301 gctgaagaag attttgtgca agcagcaaaa gaactgcaag atatgggtgc aaaaaacgtt
    12361 atgatcaaag gacatcatgc taaaaatgcg cagcaagaaa cagtcaatga ctacgtattg
    12421 ttcgaatcag gaaaatcatt ttggttaaat gcaccatttt atcctaccga ccggatcaac
    12481 ggaactggcg attcgctatc tgcatgtatt gcagctgagt tagcgaaagg aaaaacagtt
    12541 gaacaagcga tccgattggc aaaagactat gtaaaccgtg caattcgtga agaaataaat
    12601 gtagggcaca aatatggccc catcaatcat tgggcagctg gtgaattaga gtaaaagaaa
    12661 gaagttgtga aaaagttgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
    12721 ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttaa
    12781 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
    12841 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    12901 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
    12961 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
    13021 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
    13081 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
    13141 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
    13201 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
    13261 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
    13321 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
    13381 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
    13441 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
    13501 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
    13561 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
    13621 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
    13681 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
    13741 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
    13801 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
    13861 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
    13921 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
    13981 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
    14041 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
    14101 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
    14161 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
    14221 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
    14281 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
    14341 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
    14401 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
    14461 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
    14521 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
    14581 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
    14641 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
    14701 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
    14761 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
    14821 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
    14881 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
    14941 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
    15001 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
    15061 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
    15121 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
    15181 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
    15241 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
    15301 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
    15361 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
    15421 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
    15481 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
    15541 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
    15601 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
    15661 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
    15721 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
    15781 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
    15841 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
    15901 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
    15961 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
    16021 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
    16081 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
    16141 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
    16201 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
    16261 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
    16321 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
    16381 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
    16441 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
    16501 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
    16561 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
    16621 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
    16681 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
    16741 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
    16801 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
    16861 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
    16921 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
    16981 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
    17041 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
    17101 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
    17161 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
    17221 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
    17281 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
    17341 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
    17401 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
    17461 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
    17521 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
    17581 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
    17641 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
    17701 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
    17761 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
    17821 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
    17881 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
    17941 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
    18001 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
    18061 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
    18121 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
    18181 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
    18241 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
    18301 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
    18361 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
    18421 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
    18481 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
    18541 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
    18601 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
    18661 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
    18721 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
    18781 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
    18841 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
    18901 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
    18961 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
    19021 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
    19081 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
    19141 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
    19201 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
    19261 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
    19321 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
    19381 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
    19441 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
    19501 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
    19561 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
    19621 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
    19681 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
    19741 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
    19801 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
    19861 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
    19921 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
    19981 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
    20041 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
    20101 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
    20161 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
    20221 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
    20281 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
    20341 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
    20401 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
    20461 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
    20521 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
    20581 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
    20641 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
    20701 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
    20761 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
    20821 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
    20881 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
    20941 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
    21001 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
    21061 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
    21121 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
    21181 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
    21241 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
    21301 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
    21361 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
    21421 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
    21481 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
    21541 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
    21601 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
    21661 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
    21721 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
    21781 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
    21841 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
    21901 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
    21961 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
    22021 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
    22081 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
    22141 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
    22201 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
    22261 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
    22321 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
    22381 ataaagcaag atttacagtt attttattta ctgcagatct tgctttt
//
LOCUS       DAYYCM010000047        22364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000047 DAYYCM010000000
VERSION     DAYYCM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.048"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..929
                     /locus_tag="ACHAY1_001835"
     CDS             39..929
                     /locus_tag="ACHAY1_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8593822.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1002..1826)
                     /gene="nadE"
                     /locus_tag="ACHAY1_001836"
     CDS             complement(1002..1826)
                     /gene="nadE"
                     /locus_tag="ACHAY1_001836"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8593823.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1838..3310)
                     /locus_tag="ACHAY1_001837"
     CDS             complement(1838..3310)
                     /locus_tag="ACHAY1_001837"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8593824.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3947..4309)
                     /locus_tag="ACHAY1_001838"
     CDS             complement(3947..4309)
                     /locus_tag="ACHAY1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8593825.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4532..5284
                     /locus_tag="ACHAY1_001839"
     CDS             4532..5284
                     /locus_tag="ACHAY1_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8593826.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5424..6347)
                     /locus_tag="ACHAY1_001840"
     CDS             complement(5424..6347)
                     /locus_tag="ACHAY1_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8593827.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6362..6634)
                     /locus_tag="ACHAY1_001841"
     CDS             complement(6362..6634)
                     /locus_tag="ACHAY1_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593828.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(6978..8165)
                     /locus_tag="ACHAY1_001842"
     CDS             complement(6978..8165)
                     /locus_tag="ACHAY1_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8593829.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(8578..10203)
                     /gene="groL"
                     /locus_tag="ACHAY1_001843"
     CDS             complement(8578..10203)
                     /gene="groL"
                     /locus_tag="ACHAY1_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8593830.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(10254..10538)
                     /gene="groES"
                     /locus_tag="ACHAY1_001844"
     CDS             complement(10254..10538)
                     /gene="groES"
                     /locus_tag="ACHAY1_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8593831.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10763..11425
                     /locus_tag="ACHAY1_001845"
     CDS             10763..11425
                     /locus_tag="ACHAY1_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8593832.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(11501..12793)
                     /locus_tag="ACHAY1_001846"
     CDS             complement(11501..12793)
                     /locus_tag="ACHAY1_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8593833.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12963..13592
                     /locus_tag="ACHAY1_001847"
     CDS             12963..13592
                     /locus_tag="ACHAY1_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8593834.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(13695..14504)
                     /locus_tag="ACHAY1_001848"
     CDS             complement(13695..14504)
                     /locus_tag="ACHAY1_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8593835.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14559..15428)
                     /locus_tag="ACHAY1_001849"
     CDS             complement(14559..15428)
                     /locus_tag="ACHAY1_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8593836.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(15429..16745)
                     /gene="yycH"
                     /locus_tag="ACHAY1_001850"
     CDS             complement(15429..16745)
                     /gene="yycH"
                     /locus_tag="ACHAY1_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8593837.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(16742..18577)
                     /gene="walK"
                     /locus_tag="ACHAY1_001851"
     CDS             complement(16742..18577)
                     /gene="walK"
                     /locus_tag="ACHAY1_001851"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8593838.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(18582..19286)
                     /gene="yycF"
                     /locus_tag="ACHAY1_001852"
     CDS             complement(18582..19286)
                     /gene="yycF"
                     /locus_tag="ACHAY1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8593839.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(19476..20336)
                     /locus_tag="ACHAY1_001853"
     CDS             complement(19476..20336)
                     /locus_tag="ACHAY1_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8593840.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(20323..20892)
                     /locus_tag="ACHAY1_001854"
     CDS             complement(20323..20892)
                     /locus_tag="ACHAY1_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8593841.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            21273..21345
                     /locus_tag="ACHAY1_001855"
     tRNA            21273..21345
                     /locus_tag="ACHAY1_001855"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21306..21308,aa:Lys,seq:ctt)
     gene            complement(21627..22319)
                     /locus_tag="ACHAY1_001856"
     CDS             complement(21627..22319)
                     /locus_tag="ACHAY1_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8593842.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
BASE COUNT         6448 a         4654 c         3795 g         7467 t
ORIGIN      
        1 gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag
       61 aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac
      121 tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta
      181 tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag
      241 gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa
      301 tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg
      361 caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac
      421 cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat
      481 ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag atgttggaag
      541 tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca
      601 ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata
      661 tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga
      721 agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca
      781 cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg
      841 atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat tacgatcagg
      901 atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat aaaacaaaaa
      961 cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca gaaatcatcc
     1021 aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt
     1081 tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc attatatgtg
     1141 acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga
     1201 atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt
     1261 ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt cgtgaagaat
     1321 ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct ttctccagct
     1381 acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt aaaatcgctg
     1441 attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg tttgatattt
     1501 actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc ttcttcatct
     1561 gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc ttttgtttct
     1621 tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga atcttggccg
     1681 ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata tgctttcata
     1741 aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac acctaattca
     1801 ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta tgaagttctg
     1861 ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat tcagtagaca
     1921 gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat agtgaatcta
     1981 gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa actctttttc
     2041 cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca gttttattga
     2101 taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg tcttcatccc
     2161 ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg cgccacactt
     2221 gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc gtatccatca
     2281 tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg gctggctgat
     2341 cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct ttttgcatct
     2401 ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt gcttccgtaa
     2461 atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata tctccactgt
     2521 cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg cgaatcgcac
     2581 ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag tctttatggg
     2641 ttttagcgta ggccataaat gcttcataat catttccata agattggact aacgaatgag
     2701 catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg cttgttgcat
     2761 ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt tcttgtgccc
     2821 gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata cgagctgctt
     2881 ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct actaattggc
     2941 agtgcgctaa aggcccttcc acttggatta atggttcatt tgcaaaaact agttctcctt
     3001 cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc aaaaaacctt
     3061 caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac gtcagattct
     3121 ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca
     3181 tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat tcccagtatg
     3241 tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg tctgggtaag
     3301 tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca ttataacaaa
     3361 aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt tttacggata
     3421 tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg tatttaacct
     3481 aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa ttagcgtttt
     3541 ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag ccgttaagat
     3601 accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg caaaagtttt
     3661 tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg gatataactt
     3721 attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt gcgttgaaaa
     3781 aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa gcagctgtca
     3841 atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt ttattgtata
     3901 cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa atagaatcct
     3961 tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat tgtattccgg
     4021 cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt gtcattttta
     4081 aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc gtattgatca
     4141 tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa
     4201 ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact ttaggataaa
     4261 atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc tcattcctct
     4321 tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc ctagcattaa
     4381 ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc acttaatagt
     4441 ataacataat ttcgtgagaa aaaaatacaa ttttttcttg accatctcgt tacgtaaagg
     4501 attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga aaatgtcaga
     4561 gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac tgctaaaacc
     4621 agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg atcgtctgca
     4681 acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag aggtcttaga
     4741 caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa
     4801 acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata tgtacgaagg
     4861 aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga
     4921 aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa aggttacagc
     4981 atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc aaactgttga
     5041 aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg aaagcttagc
     5101 agaccgtatc ttccaagctc ataagaagtg gttctcttac agctggcctg aatatcagcc
     5161 aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca ctgcatatta
     5221 tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc
     5281 gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata acaaaacagc
     5341 agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct ttcacaagtt
     5401 ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat tttcacttta
     5461 tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc cagatggtgg
     5521 tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc aatcaccact
     5581 ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc cgaatttgta
     5641 aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc tttctctgca
     5701 tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg atgacatagc
     5761 tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt gatgcccaat
     5821 tgctttatac cttgctgctt caaataagta agaccttctg taattgattg ttctcgatca
     5881 ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag aggtccatat
     5941 ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat caatccatcg
     6001 aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttatg atagcccgtc
     6061 ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc actgatcaac
     6121 tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt cgtttttcca
     6181 taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat gaccgcaagt
     6241 atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg tgaaatagta
     6301 gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca
     6361 cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact
     6421 gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat atatcttttg
     6481 tcaaattttc gatttcattc attggtattt cttttactat attcctattc aacccttgaa
     6541 aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa taattgttcg
     6601 ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt gatactttga
     6661 gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat
     6721 tcctctttca ttattaccaa agtctcttat tttaaattga atacattata atgatactgt
     6781 aaatatatct ttattattag tgaatggagt caagtccaca atcttgtgta aaagttcaac
     6841 ctcatcaaaa gcacaatgtt ttgcgttgtg aacctaactg ccatgccttt ctttctgagg
     6901 agagaggtca ggagcagtag cgcagcgact cttgatgtct ctcctcagaa agaagagact
     6961 cgcacaggtt catggtgcta aatgttattc gaattaaatt ctaaatattt tcgtggagaa
     7021 gccagccact cagtgtcttt ttccatcaaa agtgttccaa ttatccgagt gatggaattg
     7081 atatttggaa aaatccgaat cactcgttct ctccggcgaa tctcttcgtt caaccgttcc
     7141 aacacattgg ttgtacgaat ccttcggcgg atattttcag gaaaagacaa aatttgaata
     7201 gcatcttcaa agccgttttc taaaatgtcg caagcagcac ttaattttga atcacaacca
     7261 tatttttcca agaagtgctc ttttctttca cgtgtcaatt caagttcaga ggctttaaag
     7321 atactcttca actcgtcttt tacatcagaa gaaactttct ttggcagctt atcaaaaata
     7381 tttctcaaaa agtgggcttg gcatctttgc cagctcgcac ctaaaaagtt cttgcgaaca
     7441 gccttaacta accctttgtg tgcatcagaa atgaccagtt ttacaccgaa caaacctcgt
     7501 tgaacgaacg attgaaaaac ggtatcccaa ttatcttccg attcgccgtc ttgaacatca
     7561 aaaccaagaa tcgtacgtcg tccatcagaa tcaatgccga taccaatata tacacctttt
     7621 gagacaatac gatgattttc ccttactttc atatacaggg catcacacat gaaaaatgga
     7681 tattccgagc cttctaggct ccggtttctc cactcaaaaa caaatggatc caactgtttc
     7741 atcagatttg aaacgaatga tttagaaaca gtcgcaccat ccgttagcag ttcaactgtt
     7801 ttagttactt tgcgagtgga gacaccagaa atgaccattt caatcatggc agcgatcaaa
     7861 gctttttcgc ttcgctgata tctttcaaag atagtaggac taaacttacc gtctctggtt
     7921 cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa acgaacgatc gtagtaacca
     7981 tttctttgtg attgccgttc ttctgaacgt tcataggcat ttgcctgaat atattgattt
     8041 cgttgttctt ccatcagctg attaaacatg atagtcataa gtgtttttgc ggtatcattc
     8101 gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt caaaagtaaa atgtacttga
     8161 gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta ctcctaactg
     8221 agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta atcgtgaatg
     8281 gatatttact tttaatagat aaaatattat aactattaaa aataactatt ttcattataa
     8341 ttacacacaa aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     8401 acgtataaac agcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     8461 ttgaaaagca atttccgtga atttcttctt atttcttgga gttaaacgct tctgttccag
     8521 cctcttttta tctgtctttc actaatgatc agttgtagat aagcaagttg ctttttctta
     8581 catcataccg cccatcattg atggatccat tgcaggagct gctggcgctg ctggttctgg
     8641 tttatctgca acgactgctt ctgtcgttaa taacaaggca gaaacggaag cggcattttg
     8701 caaggcagaa cgtgttactt tagttgggtc tacgatacct gcttcaacca tgtttaccca
     8761 ttctccagta gcagcattga aaccagtgcc tagttccacg ttcttcaatt tatcaacaat
     8821 cactgatcct tcataaccag cgttttcagc gatttgacga attggttctt ctaaagcacg
     8881 aacaacgatc ttgatacctg ttgccacgtc tccttctgct tctacagcag aaactttgct
     8941 gataacgttc actagagctg taccaccacc tgagaccatt ccttcttcta ctgcggcacg
     9001 tgtagcattc aatgcatctt caatgcgtaa ttttaattct ttcaattctg tttcagtagc
     9061 tgcaccaact ttaacaacag ctacaccacc tgctaattta gctaagcgtt cttgtaattt
     9121 ttcgcggtca aaatcagaag ttgtttcagc aatttggttt ttgatcaatt gcacacgagc
     9181 ttcgattgct tctttttctc cagaaccttc tacgatcgtt gtattgtctt tatctacaac
     9241 aactttagaa gcatttccta agttttcgat tgttacatct ttcaattcta aacctagatc
     9301 gtcagtaatt actgtaccac ctgtcaaaat agcgatatct tcaagcattg ctttacgacg
     9361 atcgccgaaa ccaggagctt ttacagctac tacgttgaat gttccacgga ttttgttcaa
     9421 tacaagagtt ggcaatgctt caccatctac atcgtcagca ataatcaaca atggacgtga
     9481 ttgttgcaag atttgttcta acaatggcag aatatcttgg atgttagaga tttttttgtc
     9541 tgtgatcaag atatatgggt tttctagaac agcttccatt ttgtcgttat ctgttaccat
     9601 atattgtgat aagtaaccac gatcaaactg cataccttct acaacatcta gttctgtttc
     9661 gatccctttt gattcttcga ttgtgatgac accgtcgttt ccaacttttt ccattgcatc
     9721 agcaatcaaa tgtccaactt tatcagaacc cgaagaaaca gctgctactt gtgcaatggc
     9781 ttcttttgaa tcaacaacag ttgagatatt atgcaattct tcgactgctg cttttgttgc
     9841 caattcgatc ccgcgacgaa tgcctagagg attagcacct gcagttacgt tttttaaacc
     9901 ttcgcgaacg attgcttgtg tcagaacagt tgctgtcgtt gtaccgtctc ctgcaatatc
     9961 atttgttttt gatgctactt ctgatacaag ttttgcaccc atgttttcaa aatgatcttc
    10021 taattcgatt tcttttgcga tcgtcacacc atcatttgtg atcaatggag agccgtaaga
    10081 tttttctagt acgacatttc ggcctttagg tcctaatgtt actttcactg tatctgctaa
    10141 tttatctacc ccgcgtaaca tagctgcgcg tgcgtcttct gcaaatttca attcttttgc
    10201 catgattgat tcacctcatt gattattttt gattattttt ttatcttcca aatttactca
    10261 acaatcgcca tgatatcttt tcctgcgacg atcagatatt cttttccttc gtattttact
    10321 tctgtaccgg cgtatttttc gaacataact tggtcgcctg cttttacagc ggcaggaact
    10381 ttttcaccgt tttctaacaa acggccttca ccgactgcaa caactgtccc agtttggggt
    10441 ttctctttag cagcagaagc taaaacgatt cctccgacag ttttttcttc ttcttttgcg
    10501 acttcgataa tgacgcgatc acctaatggt tttaacacga taaatccctc cgataactct
    10561 ttttttaatc tgttagcact cttaaccttc gagtgctaac ctacattttt aataatactc
    10621 attctccctc ttttttgcaa gcttttaaat caaagaaaag aaattttatt ctgacagctg
    10681 cttattgaaa ttatgatata attgaaatca atcgatataa gcttgccttg catgcttgtt
    10741 atcccttaaa aggaggaatt acatgtcttt aaaaaaatat agcttcctca gcattttttg
    10801 ctatggtcta atttttatct cacccttgat cctagcttcg ataggacttg taaaatctac
    10861 gtctgaattg atcacagcaa ctgcagtcat gtatatacta ggcgccgttg tcctagctat
    10921 tttttatttt aagcaacgtg aacccttagc gatcgaatca gcggtaaaaa aagcttctgc
    10981 tccaaagatt gttatttatg gattggttgg aatctttgtc gcattgattc tgcaaagcat
    11041 cgctgtcatg ttagaatctg tattgtttgg caaagcaacc ccttccgaga atacacaaaa
    11101 tatcatccag atgatcatgg aagcacccgc ttttattttg gcgacaactg ttgctggacc
    11161 catcatggaa gagttcgtat tccgccgcag tatactgggg atcatcagtc gatattcaaa
    11221 tttttgggtc ggagcagtga tcagctcttt gctgtttgcg tttgctcata atgatggtca
    11281 tctgctaatc tactttttcc taggtttctt cttcagccta gagtacaaag caactggtcg
    11341 gatctggaca tcgatgatca cacacgtagg aatgaacaca ttagttgttc ttgttcaatt
    11401 agctgtccaa aaagggctag tataaacatg actataaata ctcctgtcat cctagattgg
    11461 tgattttccc aatctaaagc gacaggagta tttttgtttt ttagtcgttc atcttttctg
    11521 ctgctccatc attttttgtc tgtctttcct ttttgcagtt tgcagaatct atttttttca
    11581 aaaatgggaa ataaatgaaa aaactaattg acaacacgat accttgtaaa atcgctgcag
    11641 gcactccgcc tactaagaaa cccgagagga tcggcggtgt tgtccaaggg acttggactg
    11701 ctgtaaataa aggaacgaat ccaaaatata aagcggaata ggtaatcaat cctgaaacga
    11761 ccggtgcgaa aataaaagga acagccatca tcgggttcat cacgatcggt gtggaaaaaa
    11821 taattgattc attgatgttg aaaaaggctg gtaaaagagc aagatttcct aattctctgt
    11881 atttcttcga tttcgcaaaa accgtcataa aaaatacagc ccctaacgtc aatccagcac
    11941 ccgtaaccgt catgaactga tcgagaaatt gctgagtaac gatatgtccg ccatttgcca
    12001 ccgtcaactc ttgtcctgag gctaagattt ctgcattcgc taatgaattc gcttgcaaga
    12061 cgggactaac caacccattg accatcgcag atccatgaag accaaataac cataaaaacg
    12121 gaacaaaaaa acctaataaa agcacaccaa acaacgtgtc tatgaacgat tgaatgggaa
    12181 gctggacata tgtccatact ccttgaacaa cagtcattct gaacaactgt tgaaaaaccg
    12241 ctgaaccgat aagcgtaaaa gtgactataa caatgattgg aatcaatgaa gtaaaagact
    12301 gccaaacatt ttcaggtatc ttctctcttt gcttccaccc tttcgcaagt cttactaaac
    12361 gtgcatagcc ccatccgatg atgagcccag ttgtaactgc tcctagtatc ccttcagcac
    12421 ctgtccagac agtatctatc tgagtattta gtggctgaat aagcagtaga taagcagaca
    12481 aggctgttat gccgactggt aagcgatcga tgtcttcttc ttttgcataa gcgtatgcaa
    12541 tggcaaaaac gatccaaaag gctatcacat caaatgtagc ctggtatact tgtgtaaaat
    12601 acatagaaaa tcctgttttg tctatccact gagcgatgta atccacagga aaatgagcaa
    12661 ataagaaaaa cagcgagcca acaataatta ttgggatcgt gtaagtcatt ccattacgca
    12721 aagcagttaa gggtctaaga ttcacaacag atgttatcat tggtaaaaag gcacgatcta
    12781 accactcttt catttcttat ctccttcaac ttctattttt atcaatcagt tttcttaatt
    12841 taactatacc caatgatcac gaaccatgaa aggaatgcgc actgagcttt tcaaaaatcg
    12901 gattctttga cgaatattca gcaactgctt acaatggaca ggaataaaaa aggaggtttt
    12961 ttatgaaaat tgcactcatt ggagctactg gccatgcagg ttcatttatt ttagacgaag
    13021 cattgtccag agatttagac gtgacggcaa tcgtgcgtca tccagaaaaa ttgccatcag
    13081 atgttccgtt tatacaaaag gatttatttg agttgacaac tactgatctt gcatcttttg
    13141 atgttgtcgt tgatgctttc aacgctccca aaggaaaaga agaaatgcat caaacttctt
    13201 tgtcccattt gattgagatg cttgaaggaa ccgatacacg cttaattgtc gttgggggtg
    13261 cctcttcgtt attcttagat acagaaaaga cgactcgtat gattgaccaa gtacctaaaa
    13321 atgcaccctt ctatccaaca gctttcaata tgtacgaagg attgttaaaa ttgaaacaat
    13381 ctaaaaatct acattggact tatatcagtc cagcttcttt atttgatcca catggcaaac
    13441 gtacaggtac ttatttgtta agtgatgatt atttacaaag aaacgctgct ggcgacagta
    13501 cgatcagcat ggcagattac gcgattgcat tagtagatga gatactggat gagaaacatg
    13561 aaaagcagca catcagtgta gtcagtcgat aatacggtta tttttttaat atgctaaaaa
    13621 aataaaaata gaagataaaa ccaaccctga ttgattttat cttctgcttt ttatattttt
    13681 attgattgaa ctgtttaaat cgaaaatagc tcagttgctt catctggatc tgtatcatag
    13741 atttcaaaat catgtccaac gccaaaatct ttttctttca aaacattttc cattgttaaa
    13801 tgagcaagtt ctttcatatt gttttccttg ctaagatggc caagatagat tcgtttggtc
    13861 cgattaccga tcacatcagt catgacaagt gcaccgtctt cgttggaaag atgtcctcta
    13921 tctcctaaaa tacgctgttt taaattccac ggataaggtc ccatgcgtaa catctcaaga
    13981 tcatgattac tttccatcaa ataaccatcg gcattttcaa tgacacctcg catataatca
    14041 ctgcaatatc cggtgtccgt caacatgacg aatgaccgat tttctttatg gaaacgataa
    14101 aattgcggag ccgctgcgtc atgggagacg ccgaaactct caatatcgat atcgccgaat
    14161 gttctgactt tccccatctc aaatagatgt ttttgttcca ctggtacatt tccaatcagt
    14221 ggttccatcg cttcccaagt ctgctgattg gcaaaaacat ccaaatgata cttacgagcc
    14281 aagacaccca cgccatgtat atgatctcga tgttcatgcg tcacaagtat tccatctaat
    14341 tggtccggtg tccgatcaat ttgagccatc aaggaagtga tttttttacc gctaagtcct
    14401 gcatcgacca aaagccgttt tttttcactt tcgatataaa gtgaattacc cgaacttcca
    14461 cttgctagga tactgatttt aaaagctggt ttttcttcag ccatttgact cctacttccc
    14521 ttcttacttt gtaaactttt ttcattatac tcgctttttt attgatcttc cacttttggt
    14581 acagtattgt ttgtcacgat cgtattgctg aaagcgttga cactctcaat ttggaccact
    14641 ttatcgcttg tttcgatcgc tacatgccaa acaggaacat acacattttt ttcccggact
    14701 ttcagaatac aggaataagc tagctgacgc cataaaattt ttgatcctct tggtattttg
    14761 ttattgatgt acaatgtatt gattgcttcc tcttccgtat acagttccgt cttttcgcgc
    14821 agctgctcga tatcagataa atgcgtctgc atgtatttcg tgatgctata atcattatcg
    14881 tttttttcta aagaaagaac gatttttgct gtatcatcat tgaatgggat gccttcatac
    14941 tcttggcttg cttggatttc tagtggttct tcttctaaat gagaaaagtt tttcgtgtac
    15001 acataatcat taccgaataa aatattgtct ttgttagata aaaaatcttc caaggaacta
    15061 ctaattttat ctggatcgat cacgttcttt tcagcatctg aactggataa agatttcgtc
    15121 aaaacatcac cgtctaaaaa cacaccactt gccagcaagc cgcttttacc agccttgcga
    15181 taattcgcta atgaagtgct aagattggtc tgctccgcgc taagataata gccttgcttg
    15241 ttctcagacg aaatcttccc cgtgtaagtg atatcgtcgc tagccagacg ttgttggatt
    15301 gattcagtct gattagagcg aatgacattg ttttcttggt ataccgcgtc atgataactg
    15361 ctgaataaaa acacattcaa ccctaaaaaa gcgacgaaaa atatccattc gatttttttg
    15421 aaatccaatt aattcgcctc caatccaggt aatttttcca gaaggtcgtt agctgaatac
    15481 cagttatctt tatatttcac ataccattca ggtaagagat caacgacacg attcgtttct
    15541 ttgacatttt gccatgtata gccaacgatc actgaaccaa tcttgttgct atctgctccc
    15601 ttagctttta actgctggat gatttcattc gtacttgcaa gttctacttc ttcgtctgaa
    15661 ggtatcggca cttgaatcgt attcaaactg gtttggatat tcacttgcac agaaccattc
    15721 gtttcaccgc caacttccac accaatctgt cctctgctgt ctgtcccaaa aaccgggaag
    15781 ccttccacga atatccgata atcgatcgta ttgtcactgc ggtcaaaata tctgaggttc
    15841 cctaaagaac tgcctaattt gctaatatag gggaaacttt cagaaaatac actagcattt
    15901 tcttttttct ctggcagctc tccacgaaaa tcaactactt gcgtttccga atggatagac
    15961 attgtttcat tcccaccgta aaaaatcaat tcattgcttt catcattatt tttcacgcta
    16021 tctggtttct ggaaaaaagc atttctaaaa agtgtatagg gttgctgtga caggatataa
    16081 ctatactttt tcagtttgat cgaatgtttc gtagcatatt gcgtgtcgat cgtcgatggt
    16141 tctgttgtca tttctgtcca ttttgtgtct tttgctgtta attctttatt gatatctgtc
    16201 caattcaacg aaatatttgc ttcatatacg agatggttcg aataattcaa aaatcggatt
    16261 tttttatttc cttgatcgaa ttgtattcta gtaaaataca tattttcgct ttcttttgcc
    16321 aagttcgaca catctaaatc taaatcaaat gcttccttgt attctcttag caaaaaaggc
    16381 gcattatagg ccaactcaaa accagagttc agatttttca cctgttcaaa ctgtgatttg
    16441 tctcctgaaa ctacttcaga aagctttcca aatcttgctt tatcgatgat caattgcaat
    16501 cggtacacga gattttcact gttggtttct tggatcattc ccgaatggtg ccatgtaaca
    16561 tgaagcggaa gaaagacttc ggaagttttt cgattgcttt gagactcgat catttgagtg
    16621 tttgtctcat ctacgttgat tgaacttttt cctgctgggc tcagccagat tgcataagag
    16681 aagtatatgc ttaacgcaac catcaaaatg agtcctatac gaacgacttt ttcagagatt
    16741 ctcattccca ccattcctcc tcataaggtt catatggcag cgagatataa aaagtcgacc
    16801 cttttccttc cttgctctct gcccaaatag cgcctcgatg cgccttaatc acttctctag
    16861 aaatagctaa tccaagtcct gtcccacctt gtgctcttgc acgtgcttta tctactcgat
    16921 agaaccgctc gaacactcgg ttcagatctt ttttaggaat ccccaatcct tgatcggtga
    16981 tacttaaaac cacgttatta tgcgtttcta ataagcgaca agtaatcgtt cccccatctg
    17041 gtgaatactt gatcgcgtta ttcatgatat tatcgactac ttggatgatt ttatctgtat
    17101 ctacttcgac ccaaagatct ctttgcgtaa attcacgacg gatcttataa tttttttcct
    17161 gatttcccac catcatatca aaccggtcaa gaacaaaatt cactaactcg ttgaaattga
    17221 catattctaa ttgaagctgg gtattacctg tatccatgcg agatagattt aagaggtcat
    17281 tgatcattcg aatcattcgg tctgtttcat ctagcgttac cttcaaaaaa tttggtgcta
    17341 cttcctcgtc tttccacgca ccttcgctca atgcttcgat ataactacgc atacttgtca
    17401 acggtgtacg aagttcatgg gaaacattag agacgaattc acgacgttcc cgttctgttt
    17461 tttcttgttc tgtcacatca tgcaagacag ctactaatcc gctgataaaa cctgattcgc
    17521 gacggatcat agaaaagtct actcgcaaga tcatctgatc actttccaat gtactggttg
    17581 aacggtcaag taccatttcg tttggatctt ctaataattt ccgcaacgta tattcttctt
    17641 cgatatctaa aagtgtcaaa atcgattgtc cgattgcttc ctcgtttttg atatctagca
    17701 aggaaacagc catatcattg atcgtaatga ctttacccct gcgatctgtc gcaatgacgc
    17761 cgtctgtcat atgggatagc acgctatcca aacgattgcg ttcagcttcc attgtatctt
    17821 gagcttcttc aatacgttca gctaactgat tgaaggtatc agccagctgt ccaagttcat
    17881 cttgtccatg cactttgact ttccgactgt agtctcctct agcgattcga atggcttgtt
    17941 ctctcatttc accgatcggc tgtgtaatcg aacgagcaac taaaacagat acgatcattg
    18001 aaataaatgc cgcaattagt gaggcagtaa agaaaatgac tgctgtgtta ttgatttccg
    18061 aatatttttg ttcgatatta cttttgacgt aaagagcccc gataacggca tcgccggtcg
    18121 gggacaggat cggttgaaca ttgatattca cccgtttatc gtcgttatct aaagcaacat
    18181 aacgtttagt cgtgaaatca ttgatatcaa cataatcatt tttctttccc acagcgcttt
    18241 gttcattgac atcggttgtt cctcggatga tccctttttc gtcaacgacc cgtatttcga
    18301 tgatatcgct agtcgcactg ttttcaataa atcttttgat attcgcattt acctcttcat
    18361 catctgcatt atcttttcga ttcaattccg ggctgattgt tgttgctaat gattccaccg
    18421 tttgattcat atctttaata aatgtattga tcgtggaacg ttctaaacca cgtataaagt
    18481 aggcaccaat aatttcgatc gagatcaata aaatcagaat aaaggtaata gcgatcttaa
    18541 aattgactga tcgaaaaaag cggacttttc ttttcatcca attactcctg ttctgggtta
    18601 cgcaaatagt aaccgacacc acgacgtgtc actaaataat ttggatggct tggattatct
    18661 tcgatctttt cacgcaaacg acgtacagtt acatccactg ttcgtacatc tccaaaataa
    18721 tcataacccc aaaccgtttg taataaatgt tctcttgtca tcacttgtcc aaggtgcttc
    18781 gccaagtaaa agagtagttc gaattcacga tgtgttaact cgattgtttc tcctcgtttc
    18841 gtcaccatgt aagcttctgg atggatcgtc aaatcaccaa tcgtcaattc cgcaggtgct
    18901 gcctcttctg gttccttcgc agccgtggca ccgcgacgaa gatttgcttt gacacgtgca
    18961 actaattctc ggttggaaaa tggcttggtc acgtaatcat cggcgcctaa ctctaagcct
    19021 aggactttat caatttcaga atctttggct gttaccataa tgatcggcat atcataagtt
    19081 ttgcgtacct cacgagcaac ttctaagccg tccattttag gtagcatcaa atcaagtaaa
    19141 atcaaatcag gttctacctc agcaactttt tccaaggctt cttccccatc atatgcagta
    19201 taaacatcat agccttcttt ggctaaattg aatttgacga tatccgaaat cggcttctca
    19261 tcatcgacaa ctaaaacttt tttcataaag agccacctct tatatattat tgtcatacct
    19321 tctcattata ccgtatttct gcttttgttt catctttgcc tataaaacaa aagagaaaat
    19381 taaaatgaaa gtgcaaaata ctttattatt ttttgttcta acttcaataa aaaaggaaaa
    19441 cactgatttt cacctcagtg tttccttttt ttgatttatc cttctaaaac ttgtacaccg
    19501 acacccaata atcccggacc agtgtggact actaatgccg gagaaatctg gccaaagtag
    19561 atttggtttg cttgtgggaa ctctctttta agtctcgctt ccatttcaat tgcttcttct
    19621 ttcgcatccc catgggcaac agctaagttg aagacctttg cattaccgat tttttctttg
    19681 acgtaagcaa ccgttttatc caaacttttt ttacgtcccc tagcttttcc tactgtgtaa
    19741 taaatccctt cttcattaca tgagatgatt gggttaagct ttaaagcggt tcctacaata
    19801 gaagcaacta aaccgatccg tccacctttt tgaagatatt ccaatgtcgc aacattaaag
    19861 aaaacttttg ttctttcgac taactcattc agattactga tcagttgatc ccattctact
    19921 ccttcttcca gccattttgc tgcttggatc gcagaaaaac ctgcaccaat accaatattt
    19981 ttcgtatcta gtacatatgc ttccaattct tcttgctgtt gtgcaattaa acgaaccaca
    20041 ttatatgtcc cgctcaatcc actggaaatt gttaccgcaa gtaccttttc ataaccgtct
    20101 tttttgattt gctcaaagat ttctgtgatc gactcaccat ccggcaaaga tgtaccaggg
    20161 atttcctctg gcaaacgttg atagatctct tcagaggtaa tgtcaatttt atctgtatat
    20221 acctgatctt tataaataat tttcaatggg atcatataca tctcgtattt ttttatcaat
    20281 tcttctggca catccgtacc tgaatctact aaaattgcga ttttattgtt tttcatttgt
    20341 tttctcctct tttaaatatt cggtttctag aacaatcgtt ttttccgcaa gaagtttcat
    20401 tgcaaacgat aaagttgccg ctcttacagc aatatattct acaggggttg cctggtcgaa
    20461 tttttgcgga tgagatttac cttgtgctaa ttggctgacc atccacacag ctgcttcttg
    20521 ttcatcgcaa aatagattat aggcttcacg aattcctact gtttttcctt ggaacaatat
    20581 cccttctttg atttcaggaa tagtcaatac ttgtttcaac gtagtgattg caagtaaaaa
    20641 tgctacgtgt tctttgttat atcttttttt aactggtgct ggtatcaaac ctagtttaac
    20701 gtaattgttt accatagaag aggtaagcaa tggatgcttt tctccttgta tcacaggtga
    20761 aagataacga tcaactaacg ttctgacttg gtccatataa agttctaagt cagggagttc
    20821 ttcccagtga ggcagacgga atgtttctag ttccttcccc cactccatta attgctgacg
    20881 cattttttcc atcataaccg ctcctttttt catttcataa acatgatatc agacttatga
    20941 taaacattca atctagttgc tttaactagg tgatttgatt agcgggattc catgaaaaca
    21001 aaaaacaaga aggctaagcc ttcttgtctt ttatcgttaa gaacaggaga cccgccgggg
    21061 agacggcggg aaaggagtaa aaaatgaaaa aagtgttagg gtttgttctc ttgcaagaac
    21121 tttgtttgtt ggtatgctta tataatagcg agcaaatgtg aataatctat gaatgatttc
    21181 ctataaggaa gtaaagattt ttctgatttt ctttaaatgt agtcttgcat tttaaaattc
    21241 ttagtgctac tattattaag tactttttgt acgggccgtt agctcagttg gtagagcagc
    21301 tgactcttaa tcagcgggtc gcgggttcga gcccctcacg gcccattggg tgccaaaccc
    21361 acgtcaccag tacattcttg gctacttagg tagtttcgaa aagagtgtac gtggtggcat
    21421 tttttatttt tcgggtatga aattcgggta tgcattttag tattttattt tttaataaca
    21481 tcctaatcag tcaataaacc tgtagattat cacagtatgt acttcccctc cattttgagg
    21541 ggagatatca ttgtagaata ttcaaataaa aattatttag caacaaaata ctattttgtt
    21601 aaagaaattt aaactaaaaa atgcatctat tttcctgtat gttcagtcac gatttcttgt
    21661 gatctgtttg ctgtgtcttc tacatcgctt ttggcttttt ccagtttttc aatataatta
    21721 ttcaaattac tgttttctga ttgcgctttt tctagttttt gttcagtact ttgcaattgg
    21781 tttttaatag tatttttttc atttgacaat tgattaattt gttgatttaa actattaaca
    21841 gtattttgag catcattaaa tttattttgt tctgtttgta aacgattatt ccaatcagtg
    21901 tttaattgat ttatttcagc tattttttga tttatttcat caattttagc attgatttca
    21961 ttttgtttac cttgcaattc agcatttttt cctgataatt gattctctaa atttgttttc
    22021 tctatagtta attgattttt atcattttcc aaagtcaaca ctttttgttt atattcttca
    22081 acgttagcct ttgcttgctc aagttcttga ttatttgaag ataatcgtga atttaaacta
    22141 ctaatatttt gctctttatt ttctagtgct ttatctaatg actccaaatt atgattgatt
    22201 tgttcaatat tatcttctcc accccataac aaaatgtcat cggcaaagtt tttccctgcg
    22261 atccctgcta acactaatcc acctataatt aatccactta ctacaatttt cttattcata
    22321 tcatcattct ccttaacata aactgaacta attatagtta atga
//
LOCUS       DAYYCM010000048        22325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000048 DAYYCM010000000
VERSION     DAYYCM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.049"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHAY1_001857"
     CDS             5..403
                     /locus_tag="ACHAY1_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8593843.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="ACHAY1_001858"
                     /pseudo
     CDS             387..1081
                     /locus_tag="ACHAY1_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1675..2562)
                     /locus_tag="ACHAY1_001859"
     CDS             complement(1675..2562)
                     /locus_tag="ACHAY1_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8593844.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2747..3586
                     /locus_tag="ACHAY1_001860"
     CDS             2747..3586
                     /locus_tag="ACHAY1_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8593845.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3782..4756
                     /gene="sgrA"
                     /locus_tag="ACHAY1_001861"
     CDS             3782..4756
                     /gene="sgrA"
                     /locus_tag="ACHAY1_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8593846.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5054..5410
                     /locus_tag="ACHAY1_001862"
     CDS             5054..5410
                     /locus_tag="ACHAY1_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593847.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5455..6570
                     /locus_tag="ACHAY1_001863"
     CDS             5455..6570
                     /locus_tag="ACHAY1_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593848.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6596..7036)
                     /locus_tag="ACHAY1_001864"
     CDS             complement(6596..7036)
                     /locus_tag="ACHAY1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8593849.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7196..7702
                     /locus_tag="ACHAY1_001865"
     CDS             7196..7702
                     /locus_tag="ACHAY1_001865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8593850.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7870..9783
                     /locus_tag="ACHAY1_001866"
     CDS             7870..9783
                     /locus_tag="ACHAY1_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8593851.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9788..10789
                     /locus_tag="ACHAY1_001867"
     CDS             9788..10789
                     /locus_tag="ACHAY1_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8593852.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10991..11197)
                     /locus_tag="ACHAY1_001868"
     CDS             complement(10991..11197)
                     /locus_tag="ACHAY1_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8593853.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11843..12691)
                     /locus_tag="ACHAY1_001869"
     CDS             complement(11843..12691)
                     /locus_tag="ACHAY1_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8593854.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12855..13757
                     /gene="gnd"
                     /locus_tag="ACHAY1_001870"
     CDS             12855..13757
                     /gene="gnd"
                     /locus_tag="ACHAY1_001870"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8593855.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13759..14760
                     /locus_tag="ACHAY1_001871"
     CDS             13759..14760
                     /locus_tag="ACHAY1_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8593856.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14757..15041
                     /locus_tag="ACHAY1_001872"
     CDS             14757..15041
                     /locus_tag="ACHAY1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8593857.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15074..15871
                     /locus_tag="ACHAY1_001873"
     CDS             15074..15871
                     /locus_tag="ACHAY1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8593858.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15885..16718
                     /locus_tag="ACHAY1_001874"
     CDS             15885..16718
                     /locus_tag="ACHAY1_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8593859.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16736..17593
                     /locus_tag="ACHAY1_001875"
     CDS             16736..17593
                     /locus_tag="ACHAY1_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8593860.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17615..18124
                     /locus_tag="ACHAY1_001876"
     CDS             17615..18124
                     /locus_tag="ACHAY1_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8593861.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18161..18562
                     /locus_tag="ACHAY1_001877"
     CDS             18161..18562
                     /locus_tag="ACHAY1_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593862.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18674..19027
                     /locus_tag="ACHAY1_001878"
     CDS             18674..19027
                     /locus_tag="ACHAY1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593863.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19061..19504
                     /locus_tag="ACHAY1_001879"
     CDS             19061..19504
                     /locus_tag="ACHAY1_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593864.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19521..20606
                     /gene="uxuA"
                     /locus_tag="ACHAY1_001880"
     CDS             19521..20606
                     /gene="uxuA"
                     /locus_tag="ACHAY1_001880"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8593865.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20599..21312
                     /locus_tag="ACHAY1_001881"
     CDS             20599..21312
                     /locus_tag="ACHAY1_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8593866.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21519..21668)
                     /locus_tag="ACHAY1_001882"
     CDS             complement(21519..21668)
                     /locus_tag="ACHAY1_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593867.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22064..22264
                     /locus_tag="ACHAY1_001883"
     CDS             22064..22264
                     /locus_tag="ACHAY1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8593868.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7689 a         3430 c         4623 g         6583 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1501 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1561 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1621 tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat ttgtttattt
     1681 gaatatattt tttacataag gatctttaat agttacattg tcttttttca tcactttacg
     1741 gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa ttttttctag
     1801 ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca tttttactag
     1861 ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag aaacttcccc
     1921 gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat tggttgaatc
     1981 aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag cttgaacaag
     2041 cttggagaaa ttttctcctt catttgcttc tttttttgct ttatctgctt cttctttagt
     2101 cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag cagtgtctag
     2161 atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca gcatctgttt
     2221 aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt ccttgtagtt
     2281 tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg aagaaatctc
     2341 ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata gtgtttgatt
     2401 atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc cacctttaaa
     2461 cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg cacatgctga
     2521 aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct tattaaaaac
     2581 agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct gtagatattc
     2641 ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt ttcacgagac
     2701 aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga tcaggattat
     2761 tcggttgatt ggatctgttt taatagtctt tgttggtttt gttttaggaa ttcttgtcaa
     2821 taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa ttgccggtac
     2881 ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg tcgaaggaaa
     2941 aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt tagcgggagg
     3001 tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt tgacgggggc
     3061 gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt ttattttatt
     3121 tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc atcagatgct
     3181 tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc ttttgcacgg
     3241 attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag gttgcttttt
     3301 cagcgcactt atctacagta caggaagttt atttacggct attggtgctc atattacttg
     3361 gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag aacaaggttt
     3421 tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact ttggggctga
     3481 agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt tactctatgc
     3541 atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga tgaagaaagc
     3601 aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa aagtaaacaa
     3661 attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc gtgttctcat
     3721 cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga gagtgataga
     3781 aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa ttgcagcaat
     3841 gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat ttactgtgaa
     3901 aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag tcaaaccaaa
     3961 caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg atggtaatgg
     4021 aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga ccgctggtat
     4081 tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga gtaaagataa
     4141 agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt tgaaaccaag
     4201 cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg aaagcagtac
     4261 agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaatta gcacagaaaa
     4321 cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag gaagcacgag
     4381 cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca tgagtagtga
     4441 gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata atgaacttga
     4501 tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca acctaaaaag
     4561 tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa aagcgaaaga
     4621 aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga acaagctaat
     4681 tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc gttctctctt
     4741 ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa cgatagattt
     4801 ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag ctttgccaga
     4861 tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac aaatggaaaa
     4921 taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga aaaatcagta
     4981 acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata atagataaaa
     5041 aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag tatatcaatt
     5101 tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata tcgctttaga
     5161 tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat cgttttgcga
     5221 aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa aagtcgagtt
     5281 ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa cgattggtta
     5341 tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa
     5401 aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac tattttgaaa
     5461 aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga agaataccgt
     5521 atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca ttctgatata
     5581 ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat
     5641 acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt gcaacgaatg
     5701 aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt gcatccattg
     5761 ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc aaacttctgg
     5821 tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt tggtgtaatg
     5881 aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga aagttggggc
     5941 tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg ttttacaact
     6001 tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc aagggttttt
     6061 gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga ttttcagcat
     6121 atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc tcgcagaatt
     6181 ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata ctgtatgtta
     6241 gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca atcaaagtat
     6301 ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga acgcccaata
     6361 aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta tcctatcgac
     6421 aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca acctacaaaa
     6481 aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa aaatagatcc
     6541 atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc caaaattaag
     6601 acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc aagagttcgt
     6661 tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct tcgctaaatc
     6721 cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa gaaggggatg
     6781 tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga acaagatttt
     6841 tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc aattttagta
     6901 agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg ataaacggta
     6961 tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat
     7021 acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa taacatgaag
     7081 aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca cgtataatgg
     7141 aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca caagtatgtc
     7201 aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc
     7261 gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca aaacatacga
     7321 aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag tgccacaaac
     7381 aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt ttgtgagaaa
     7441 aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg aattggactg
     7501 tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga ctgtgaagtt
     7561 tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa cttatgataa
     7621 tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg ataaacgatt
     7681 tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg
     7741 tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat ttttatggga
     7801 taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct cctagaaaca
     7861 taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt ggtcatctta
     7921 tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat ttcttccgaa
     7981 tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga aacgaaatcg
     8041 agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt tactagcaca
     8101 gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac agctacaaaa
     8161 atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat
     8221 gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta cttcggtaaa
     8281 cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt aatagagtta
     8341 ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat caaacatctg
     8401 attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg
     8461 cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta tccaaataca
     8521 aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga tgtatatgca
     8581 tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt tcgagggaat
     8641 tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg gtataattta
     8701 ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac ctatggacaa
     8761 tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg gccatctcaa
     8821 aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt attagcgaaa
     8881 gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc aattgatgaa
     8941 caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc cataaaaaat
     9001 cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct tttgaaacaa
     9061 tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac gattggaatg
     9121 tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt acctgtgtct
     9181 aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt agtagaagga
     9241 gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca tgtaacggac
     9301 tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga aaacaataaa
     9361 agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga aaattatatg
     9421 acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg
     9481 atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc agtagctcca
     9541 ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga acctcgagag
     9601 gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca
     9661 gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat
     9721 tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg
     9781 taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat
     9841 tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag
     9901 aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca
     9961 aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa
    10021 taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat
    10081 gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct
    10141 atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa
    10201 acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa
    10261 agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag
    10321 tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc
    10381 gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc
    10441 tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc
    10501 ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag
    10561 acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac
    10621 gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt
    10681 agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa
    10741 acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat
    10801 gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca
    10861 agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg
    10921 caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa
    10981 attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg
    11041 cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata
    11101 ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac
    11161 tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa
    11221 ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa
    11281 atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca
    11341 atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat
    11401 ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg atgtatccta
    11461 aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca aatgataacc
    11521 taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa aatttttttt
    11581 aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta
    11641 ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt
    11701 ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct
    11761 tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt
    11821 tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag
    11881 cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca
    11941 aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag
    12001 aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa
    12061 taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg
    12121 attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca
    12181 tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat
    12241 gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag
    12301 attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt
    12361 catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg
    12421 ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg
    12481 ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag
    12541 agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg
    12601 gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg
    12661 ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta
    12721 tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa
    12781 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
    12841 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
    12901 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
    12961 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
    13021 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
    13081 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
    13141 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
    13201 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
    13261 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
    13321 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
    13381 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
    13441 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
    13501 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
    13561 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
    13621 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
    13681 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
    13741 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
    13801 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
    13861 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
    13921 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
    13981 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
    14041 caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat
    14101 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
    14161 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
    14221 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
    14281 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
    14341 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
    14401 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
    14461 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
    14521 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
    14581 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
    14641 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
    14701 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
    14761 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
    14821 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
    14881 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
    14941 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
    15001 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
    15061 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
    15121 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
    15181 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
    15241 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
    15301 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
    15361 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
    15421 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
    15481 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
    15541 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
    15601 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
    15661 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
    15721 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
    15781 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
    15841 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
    15901 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
    15961 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
    16021 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
    16081 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
    16141 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
    16201 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
    16261 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
    16321 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
    16381 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
    16441 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
    16501 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
    16561 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
    16621 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
    16681 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
    16741 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
    16801 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
    16861 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
    16921 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
    16981 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
    17041 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
    17101 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
    17161 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
    17221 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
    17281 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
    17341 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
    17401 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
    17461 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
    17521 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
    17581 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
    17641 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
    17701 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
    17761 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
    17821 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
    17881 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
    17941 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
    18001 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
    18061 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
    18121 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
    18181 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
    18241 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
    18301 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
    18361 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
    18421 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
    18481 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
    18541 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
    18601 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
    18661 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
    18721 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
    18781 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
    18841 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
    18901 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
    18961 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
    19021 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
    19081 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
    19141 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
    19201 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
    19261 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
    19321 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
    19381 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
    19441 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
    19501 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
    19561 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
    19621 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
    19681 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
    19741 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
    19801 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
    19861 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
    19921 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
    19981 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
    20041 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
    20101 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
    20161 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
    20221 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
    20281 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
    20341 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
    20401 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
    20461 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
    20521 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
    20581 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
    20641 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
    20701 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
    20761 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
    20821 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
    20881 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
    20941 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
    21001 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
    21061 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
    21121 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
    21181 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
    21241 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
    21301 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
    21361 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
    21421 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
    21481 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
    21541 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
    21601 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
    21661 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
    21721 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
    21781 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
    21841 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
    21901 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
    21961 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
    22021 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
    22081 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
    22141 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
    22201 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
    22261 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
    22321 aaatt
//
LOCUS       DAYYCM010000049        22222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000049 DAYYCM010000000
VERSION     DAYYCM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.050"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..895
                     /gene="purC"
                     /locus_tag="ACHAY1_001884"
     CDS             167..895
                     /gene="purC"
                     /locus_tag="ACHAY1_001884"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8593869.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            895..1149
                     /gene="purS"
                     /locus_tag="ACHAY1_001885"
     CDS             895..1149
                     /gene="purS"
                     /locus_tag="ACHAY1_001885"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8593870.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1151..1822
                     /gene="purQ"
                     /locus_tag="ACHAY1_001886"
     CDS             1151..1822
                     /gene="purQ"
                     /locus_tag="ACHAY1_001886"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8593871.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1823..4045
                     /gene="purL"
                     /locus_tag="ACHAY1_001887"
     CDS             1823..4045
                     /gene="purL"
                     /locus_tag="ACHAY1_001887"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8593872.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4030..5469
                     /gene="purF"
                     /locus_tag="ACHAY1_001888"
     CDS             4030..5469
                     /gene="purF"
                     /locus_tag="ACHAY1_001888"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8593873.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5492..6544
                     /gene="purM"
                     /locus_tag="ACHAY1_001889"
     CDS             5492..6544
                     /gene="purM"
                     /locus_tag="ACHAY1_001889"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8593874.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6541..7119
                     /gene="purN"
                     /locus_tag="ACHAY1_001890"
     CDS             6541..7119
                     /gene="purN"
                     /locus_tag="ACHAY1_001890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8593875.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7116..8651
                     /gene="purH"
                     /locus_tag="ACHAY1_001891"
     CDS             7116..8651
                     /gene="purH"
                     /locus_tag="ACHAY1_001891"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8593876.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8667..9941
                     /gene="purD"
                     /locus_tag="ACHAY1_001892"
     CDS             8667..9941
                     /gene="purD"
                     /locus_tag="ACHAY1_001892"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8593877.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10063..10977
                     /locus_tag="ACHAY1_001893"
     CDS             10063..10977
                     /locus_tag="ACHAY1_001893"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8593878.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11110..12675)
                     /locus_tag="ACHAY1_001894"
     CDS             complement(11110..12675)
                     /locus_tag="ACHAY1_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593879.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12668..14119)
                     /locus_tag="ACHAY1_001895"
     CDS             complement(12668..14119)
                     /locus_tag="ACHAY1_001895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8593880.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14133..15149)
                     /locus_tag="ACHAY1_001896"
     CDS             complement(14133..15149)
                     /locus_tag="ACHAY1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593881.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15369..16457
                     /gene="chvE"
                     /locus_tag="ACHAY1_001897"
     CDS             15369..16457
                     /gene="chvE"
                     /locus_tag="ACHAY1_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8593882.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16511..18037
                     /gene="mmsA"
                     /locus_tag="ACHAY1_001898"
     CDS             16511..18037
                     /gene="mmsA"
                     /locus_tag="ACHAY1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8593883.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18039..19244
                     /gene="mmsB"
                     /locus_tag="ACHAY1_001899"
     CDS             18039..19244
                     /gene="mmsB"
                     /locus_tag="ACHAY1_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8593884.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19501..20478
                     /gene="guaC"
                     /locus_tag="ACHAY1_001900"
     CDS             19501..20478
                     /gene="guaC"
                     /locus_tag="ACHAY1_001900"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8593885.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20742..22166
                     /locus_tag="ACHAY1_001901"
     CDS             20742..22166
                     /locus_tag="ACHAY1_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8593886.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7175 a         3741 c         5057 g         6249 t
ORIGIN      
        1 tataagattt tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta
       61 catatttttg ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa
      121 aaaacgatgt atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga
      181 acttttgtat gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg
      241 gatagaatat ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg
      301 aaaaggagaa ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg
      361 aataaaaaat cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca
      421 aattatccct ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct
      481 aggtgttccc gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga
      541 ccagttggat gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc
      601 ggaagaaatc agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt
      661 atttgctaag gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg
      721 acaaattttg cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac
      781 gaatgaacat atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta
      841 tcaagaagta tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat
      901 ttcgtaaaag tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta
      961 aaaaaagcag tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac
     1021 tttgagatac aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt
     1081 gacaagctgc ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag
     1141 gaagtataac atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat
     1201 gttatgggca gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc
     1261 ctctttagaa ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt
     1321 gagatgcggt gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga
     1381 agaagggaaa ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact
     1441 tttgccaggt gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt
     1501 gaaagtcaac cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct
     1561 tccagttgct catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa
     1621 aaaaaataat cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat
     1681 tgcagggatt acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc
     1741 agtagaagac ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa
     1801 cttcggaaag gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa
     1861 gaaaaaaaga atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga
     1921 agatatctta ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag
     1981 cgaacattgt tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc
     2041 tcatgtgttg caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc
     2101 agttgtcttc aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc
     2161 tgcgacagga gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc
     2221 gttgctagat tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga
     2281 agaagtagtt gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg
     2341 cgaaattgca tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg
     2401 tttgattgaa cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat
     2461 gtatgtaggt gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga
     2521 atttaatcaa gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga
     2581 aaagctgctg cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg
     2641 tatacaggat atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc
     2701 tggctcagga cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc
     2761 ttatgaaatg atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga
     2821 ggaagaaaaa atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa
     2881 agtaacagac gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc
     2941 agtcgatgca ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg
     3001 aatcgtagaa tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt
     3061 attgcttgat ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta
     3121 tgattcacaa gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag
     3181 ggttagaggg acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta
     3241 tttggatcca aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc
     3301 tagcggtggt aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc
     3361 agaagttttt tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt
     3421 aggaactcct gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat
     3481 ctatccaaca ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac
     3541 tcaggaattc aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt
     3601 tgatggcagt gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt
     3661 tgatctatcc attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt
     3721 gattgaaagt gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc
     3781 tttcaaacat cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc
     3841 agaaactcaa tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga
     3901 aatgatggga gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat
     3961 ttcagcaact gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga
     4021 tgcaatcgta tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt
     4081 tggggtcatc cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga
     4141 ggacaagaag gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat
     4201 ttggggctgc tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa
     4261 gctgcaatcg gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag
     4321 ccttttctat tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc
     4381 aatgctcgtt cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc
     4441 gatacggaga tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa
     4501 ttaaaaagtg ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg
     4561 gcaatgattg ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa
     4621 aacggagcat acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt
     4681 ataagagatg tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat
     4741 tgctttacga aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga
     4801 ccagattcaa acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat
     4861 ttagcgaaag aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg
     4921 tcagccgcaa gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa
     4981 aatcaataca ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt
     5041 cgaatgaaat tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat
     5101 gattcaattg ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg
     5161 gcaaaagaag tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg
     5221 attgatattc aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa
     5281 gaatgtatcg gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc
     5341 ggtctggaaa aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat
     5401 ccgacgccgt tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg
     5461 ttcttttaaa agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa
     5521 agctggcgta gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa
     5581 aaaaacggaa cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt
     5641 atcggtcttt caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa
     5701 attagcagtg gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat
     5761 gtgtgtcaat gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc
     5821 aacggggaaa aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg
     5881 tgttcaagga aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc
     5941 aggtaatgat tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt
     6001 gacaggagaa acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca
     6061 ttcaaatggg tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc
     6121 atcaaaactg ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat
     6181 ctatgtttct tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat
     6241 cactggtgga gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca
     6301 aatcaaactt ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga
     6361 aattcctgag atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt
     6421 ccaagaatcg aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta
     6481 tgaaatcggg aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg
     6541 atgagaatcg ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat
     6601 ctatcgaaaa aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca
     6661 tatgttttga aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa
     6721 tttgattcaa aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc
     6781 gatttgatcg tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat
     6841 gataaaagaa tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc
     6901 atacgtgatg cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac
     6961 caaggtgtag atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac
     7021 acgttcgatt ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg
     7081 attagtgaaa ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat
     7141 cagataaaac tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca
     7201 tttcaacagg aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg
     7261 aagaagtgac agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa
     7321 tccatggtgg tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg
     7381 aaaatatcca accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta
     7441 tgaaaccaga tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta
     7501 tgctccgtag tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact
     7561 atgaagaagt actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa
     7621 aactagcggc aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact
     7681 taacggattg ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac
     7741 aaacgcttcg ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc
     7801 tggaaaaata ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata
     7861 atatccgaga tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag
     7921 tcgccttgaa acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt
     7981 gggaagcagc gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc
     8041 gagaagtaga tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg
     8101 ccccttctta tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac
     8161 tgacagtaga ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag
     8221 gtggacttct tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg
     8281 tgacaaaacg taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg
     8341 tcaaacatgt gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg
     8401 gagccgggca gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga
     8461 aaatggaagg tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat
     8521 atgcagccaa acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag
     8581 attctattga tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt
     8641 ttagacatta gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg
     8701 aacatgcaat aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc
     8761 caggaaatcc ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc
     8821 attcagcgtt aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg
     8881 aagttccttt gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg
     8941 gtccgacaaa agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg
     9001 atacttatca aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag
     9061 cgtatatcaa agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga
     9121 aaggtgtagt cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga
     9181 accagcatcg atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag
     9241 aagaattctc ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc
     9301 aagatcataa gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt
     9361 attcaccagt accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa
     9421 aaccagcggc tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg
     9481 gtttgattgc tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc
     9541 ctgaaacgca agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt
     9601 tattagagaa tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg
     9661 tggttgcagc tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc
     9721 tggaagaaga gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg
     9781 gagcaggagg acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga
     9841 aaaaaattta caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata
     9901 ttggagaaaa agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat
     9961 aaaaaataca acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt
    10021 gtggtacact tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta
    10081 ggtacttctg atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg
    10141 gaagatccag taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat
    10201 gcagatattt atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca
    10261 tcaatcaaac gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg
    10321 tttgattttt ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa
    10381 atggattatg tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa
    10441 gtagctgaag cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc
    10501 aatcaaaaac caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct
    10561 aatcagctgc aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat
    10621 atgacagatg aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat
    10681 gatatgacga tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc
    10741 gggaacgaaa aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa
    10801 acgacaccaa ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg
    10861 atcgccggta cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat
    10921 atgtctcggg aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga
    10981 ttgtcataag caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt
    11041 ttcagcttcg agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga
    11101 tttcgtttat tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga
    11161 aatcccttgt tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc
    11221 aaaagcaata tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat
    11281 tctttgttct gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact
    11341 gaaatgagag ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc
    11401 ggtaaaattt tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct
    11461 tgaaggctgt gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta
    11521 aagcgagtcg tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc
    11581 ttgcgggatt ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa
    11641 acgatataat cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt
    11701 agatacttcg tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga
    11761 atcaactgga agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag
    11821 ggaactggcg gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat
    11881 tctttctgcg acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc
    11941 atggaccaac gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc
    12001 aactattaaa aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc
    12061 cctaaataaa aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac
    12121 ttggtttgct gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc
    12181 agcaagtttc aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg
    12241 attttttttc aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc
    12301 taaagcagtt actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag
    12361 cagataatca tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc
    12421 actaataaat atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa
    12481 cccgtccatg aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat
    12541 tgaagacagt gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga
    12601 accaaggaca gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat
    12661 aaaaacttta tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg
    12721 tgacagttgt tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt
    12781 aataaagaag gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat
    12841 cgatcccttt ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc
    12901 cgttgtctat aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa
    12961 atcctttgtg acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca
    13021 agatcatttt taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa
    13081 gcatatggcc gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt
    13141 tttcaagagg aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg
    13201 catgggtcat agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat
    13261 ccaaactgtt ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt
    13321 gtgctaaatg gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca
    13381 ttttattcat gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa
    13441 agccaaggtt ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt
    13501 ggacctttct ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa
    13561 ctccaagttc cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc
    13621 ttgctaagtc gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga
    13681 cagaatctac ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat
    13741 tattgatcag ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta
    13801 acgtactaat ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa
    13861 gttcttctag gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt
    13921 cccacatact ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga
    13981 tatttgcgat cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta
    14041 gcaaagggat aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta
    14101 tgcttaaatt ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct
    14161 atattctgct gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct
    14221 gtttgaatat ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt
    14281 ttacactcga ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt
    14341 ccatcaatag ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca
    14401 gctaaagcac cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa
    14461 tcatttgttt ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg
    14521 acaattcggt gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg
    14581 gttggtttac gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag
    14641 ttctctgaac cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga
    14701 ttgctgacaa ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg
    14761 acgaaaagct cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct
    14821 gctcgctttg cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg
    14881 atcatatcca ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc
    14941 atgtagccat ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat
    15001 tcagtatgct ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt
    15061 gggtcaggtg cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca
    15121 agggctaaaa aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac
    15181 tatcttatca gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag
    15241 aaacttaaaa aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata
    15301 aaaaaatcaa tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg
    15361 gataaaaaat gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa
    15421 gcattctatt aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag
    15481 gaatcgctat gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct
    15541 cggaattaga aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa
    15601 atcaagtagc acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt
    15661 ctatcgatgg ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg
    15721 tgatcgccta tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg
    15781 ataattttgg agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag
    15841 aaggaaaagg accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac
    15901 taatcaatta caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg
    15961 tcgtgccatc tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta
    16021 cggcgcaagc aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg
    16081 ctgttttgtc gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg
    16141 ggtatgggag tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg
    16201 gcgttaaatc gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct
    16261 tagcaaaaaa tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca
    16321 atgatactga aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag
    16381 tatctgtcga taaagataat tatcaagcag agctgatcga caccgattat tataaagaat
    16441 ccgatctgaa aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag
    16501 gataagaatt atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg
    16561 aatccgagcg ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg
    16621 cggagaaaac ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata
    16681 tggctcctat gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa
    16741 agatagtgaa gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct
    16801 ttcaataaaa gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg
    16861 ggaactgaca gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc
    16921 gaatcttcta gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc
    16981 attatcaaaa tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga
    17041 agaaagcgcg aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat
    17101 cattatttca cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg
    17161 ggatggacag acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg
    17221 cggaatggtg ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga
    17281 agtttatttt aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat
    17341 aaacgaccac atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat
    17401 gggagcgggc agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat
    17461 atccggacgt atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat
    17521 tgcaaacgga ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat
    17581 ggatattcgg gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt
    17641 agataaagaa aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac
    17701 aaactccatc catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt
    17761 atccaagtgg ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat
    17821 agatgttggg gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa
    17881 agctgtctgt cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta
    17941 tacgatgaac gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt
    18001 cttgatgaaa ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta
    18061 cggataaaaa agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct
    18121 acagcatggt gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta
    18181 ttttctggcg tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac
    18241 tagctgcagg gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag
    18301 tgatggcgtt tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt
    18361 ggatgacagt tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt
    18421 gggtagctta tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc
    18481 gtggactgac acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc
    18541 aaaaaatgtc tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga
    18601 cattgatttt aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa
    18661 atcgtcggaa aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg
    18721 tatttacagc agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc
    18781 cgattatttt gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa
    18841 cagtagcagg tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag
    18901 ggatcaaaac aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt
    18961 tagctggatt agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga
    19021 tggaactgga tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg
    19081 gaacaatcat gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt
    19141 cgattttagg cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag
    19201 ccgttgtgct agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt
    19261 cagaaaaaag actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt
    19321 cattgtttcg tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc
    19381 tctttttgct cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag
    19441 ccttgattaa attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta
    19501 atgaaagtat ttgattacga agatattcaa ttaattccta ataaatgtat cgtaaatagc
    19561 cgttctgaat gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg
    19621 cctgccaaca tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt
    19681 tatttctata tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg
    19741 aaaaagcgtg gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt
    19801 atagagaagt tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat
    19861 ggacatgcta actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact
    19921 tttgtgattg ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc
    19981 gcagatgcaa caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca
    20041 ggctttggga ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc
    20101 aagccgatca tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt
    20161 tttggtgcga caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc
    20221 gaaacaaaag tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc
    20281 caaaaaggcg aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct
    20341 ttgaaagata cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga
    20401 ggaagagatc tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt
    20461 aatggagaca caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg
    20521 cattaagtaa taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa
    20581 ttattatcgc agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga
    20641 tttttgtcac agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg
    20701 taaaatcaaa agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga
    20761 aaaaacaagg accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg
    20821 gaaggatcaa tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg
    20881 tgaatcgttc caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga
    20941 gcaaacgtgg cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa
    21001 gaatcgattg gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa
    21061 aaataagaga agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga
    21121 tgccagatga gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag
    21181 cggaagaaaa agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt
    21241 atttggaaag taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg
    21301 gtcagcaagc aatctttttg attttacaaa cgattctttc agctggagag gggatagctg
    21361 tagagtcacc ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat
    21421 ttgggatacc gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata
    21481 aaaataaatt gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga
    21541 tgagtcaaaa acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta
    21601 ttgaagacga tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac
    21661 gaacgattga tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa
    21721 caacgaaaat cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc
    21781 aagaaatgat ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta
    21841 atgaagcaat tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac
    21901 tgataagcgc ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt
    21961 actatgcctg gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag
    22021 tagatatggg tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca
    22081 gaatcaatta cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa
    22141 ttctagaaaa gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag
    22201 cttggattta aatttcaagc tg
//
LOCUS       DAYYCM010000050        20898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000050 DAYYCM010000000
VERSION     DAYYCM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.051"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..542
                     /locus_tag="ACHAY1_001902"
     CDS             225..542
                     /locus_tag="ACHAY1_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8593887.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(633..998)
                     /locus_tag="ACHAY1_001903"
     CDS             complement(633..998)
                     /locus_tag="ACHAY1_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593888.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1206..1688
                     /locus_tag="ACHAY1_001904"
     CDS             1206..1688
                     /locus_tag="ACHAY1_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593889.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1885..2775
                     /locus_tag="ACHAY1_001905"
     CDS             1885..2775
                     /locus_tag="ACHAY1_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8593890.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3101..5575
                     /locus_tag="ACHAY1_001906"
     CDS             3101..5575
                     /locus_tag="ACHAY1_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8593891.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5678..7414)
                     /locus_tag="ACHAY1_001907"
     CDS             complement(5678..7414)
                     /locus_tag="ACHAY1_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8593892.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7411..8115)
                     /locus_tag="ACHAY1_001908"
     CDS             complement(7411..8115)
                     /locus_tag="ACHAY1_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8593893.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8246..8749
                     /locus_tag="ACHAY1_001909"
     CDS             8246..8749
                     /locus_tag="ACHAY1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8593894.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8709..9047
                     /locus_tag="ACHAY1_001910"
     CDS             8709..9047
                     /locus_tag="ACHAY1_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8593895.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9068..10486
                     /gene="ffh"
                     /locus_tag="ACHAY1_001911"
     CDS             9068..10486
                     /gene="ffh"
                     /locus_tag="ACHAY1_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8593896.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10598..11176
                     /locus_tag="ACHAY1_001912"
     CDS             10598..11176
                     /locus_tag="ACHAY1_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8593897.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11180..11701
                     /locus_tag="ACHAY1_001913"
     CDS             11180..11701
                     /locus_tag="ACHAY1_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8593898.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11780..12334)
                     /locus_tag="ACHAY1_001914"
     CDS             complement(11780..12334)
                     /locus_tag="ACHAY1_001914"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8593899.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12587..12862
                     /gene="rpsP"
                     /locus_tag="ACHAY1_001915"
     CDS             12587..12862
                     /gene="rpsP"
                     /locus_tag="ACHAY1_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8593900.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12874..13125
                     /locus_tag="ACHAY1_001916"
     CDS             12874..13125
                     /locus_tag="ACHAY1_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8593901.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13249..14040
                     /locus_tag="ACHAY1_001917"
     CDS             13249..14040
                     /locus_tag="ACHAY1_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593902.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14210..14731
                     /gene="rimM"
                     /locus_tag="ACHAY1_001918"
     CDS             14210..14731
                     /gene="rimM"
                     /locus_tag="ACHAY1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8593903.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14721..15482
                     /gene="trmD"
                     /locus_tag="ACHAY1_001919"
     CDS             14721..15482
                     /gene="trmD"
                     /locus_tag="ACHAY1_001919"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8593904.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15618..15965
                     /gene="rplS"
                     /locus_tag="ACHAY1_001920"
     CDS             15618..15965
                     /gene="rplS"
                     /locus_tag="ACHAY1_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8593905.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16275..16517
                     /locus_tag="ACHAY1_001921"
     CDS             16275..16517
                     /locus_tag="ACHAY1_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8593906.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16605..16751)
                     /locus_tag="ACHAY1_001922"
                     /pseudo
     CDS             complement(16605..16751)
                     /locus_tag="ACHAY1_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16808..17122
                     /locus_tag="ACHAY1_001923"
                     /pseudo
     CDS             16808..17122
                     /locus_tag="ACHAY1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17151..18410
                     /locus_tag="ACHAY1_001924"
     CDS             17151..18410
                     /locus_tag="ACHAY1_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593907.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18463..19359
                     /locus_tag="ACHAY1_001925"
     CDS             18463..19359
                     /locus_tag="ACHAY1_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593908.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19370..20239
                     /locus_tag="ACHAY1_001926"
     CDS             19370..20239
                     /locus_tag="ACHAY1_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8593909.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20249..20898
                     /locus_tag="ACHAY1_001927"
                     /pseudo
     CDS             20249..20898
                     /locus_tag="ACHAY1_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6891 a         3600 c         4278 g         6129 t
ORIGIN      
        1 aaatttgtat ttgtcctttt taggtaactt attttaatct atataccttt ctggatcaac
       61 gaaagtatac tttatatagt cataacgccg atggtaacta tgcactgtaa cgataatgct
      121 gctaaaatat ctttttacga aaaacatcac aaggaaaaac ttaactatgg tattatctaa
      181 acataatttt ttcggtattt aatatttaat taggaggtaa tattttgctt ttttatagta
      241 attttatctt aatagtagcg atattattac ttctgaatat atggattttt gataggagta
      301 gaaatgcaag tataggtttt agaactaaac gaagtctgtc ttctaaaaaa aattgggtat
      361 actcacaaac tattttttat ggaggaattg ttttaattag tttactttct tcaacacttt
      421 attctttgaa tattatagat gtttccacct caaattcaat atctattatt ggaatcatta
      481 ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat
      541 agaaaaacta gtgatagttt tggtgatata agaaaaacat atatgtataa aaagaagaaa
      601 gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt tttataagat
      661 aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc tgcaagaagc
      721 aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt aaaattggcg
      781 caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga tataatacgc
      841 ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt gatgaacccg
      901 cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg aacaaaagat
      961 aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca cctaacaagt
     1021 gttattattt tgttagtttt attgtaaatt gaatcctgca ataatcaatt actttttcat
     1081 tatttctaga ttaaatgaat aaactctcat gccattatct ctaaaaaaaa agtttttccc
     1141 cctaattttt ttagatagaa aattgtatag tatgatgaga gaaaaagtag tttgggggaa
     1201 cgttaatgag aagaaaagca gtaatcgatc ggctttattt atttttttgg ttttttccta
     1261 tcgttttgct accttttaca tgggtaaatg ctgttgtaca aggtgcggag atattaggcc
     1321 tgatccaagg aagcggatta gtggtttcta ggaattttcc agctttgact gttttctatt
     1381 tagtgataag tgtaggtctc ttattaccaa aatctctgat caatcactac aattggcagc
     1441 tgctttactt ttcttgctta ttagtattta catctatgtt ttctttgctg cctcgcttga
     1501 ttatcggccc taatggtttg agcgagagtg gattgaagga ttcagcttat tcctccatgt
     1561 atttcttgac gatcaaacca tggttctatt tagtaattgc ctcatttatt cttgcaacat
     1621 ttttttactt ctggacagaa atcaggataa aaagtgaacg atctgtatct ataaaaaata
     1681 actattgatg gtggatcatc agtaaaaaag catatgagct gcgtaaaatg catgtttctc
     1741 atatgctttt tttattatta tgtaaagaat gcgaaaaacg tttacatgat ttttacataa
     1801 cttaactata tttttacagc aaccatacga gggatttaca gtcgactgat aaactttagt
     1861 tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt ttactctagg tatgatggga
     1921 attcttttag ccggatgcgg ctcaggatca ggaaatacag atggctctgc cacacaaaat
     1981 aattcaagtg actcaaatga acaagtaaaa atcgttgcgg taggttcgac agcattacag
     2041 ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa atccaaatta tcaaatctct
     2101 gtacaaggag gaggaagcgg aactggtttg agccaagtcg aagctggagc agttactatc
     2161 ggaaactctg atgtatttgc agaagaaaga gacggagttg atgcttcaaa attagtggat
     2221 cataaagtag cagtggtcgg gatggcacca atcgtcaata aagatacaga tgtcaaagat
     2281 atcaccaaac aggaattgat tgatatcttt acaggaaaaa tcactaactg gaaggaagtt
     2341 ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa acggcagcgg tacccgcgcg
     2401 acattcgaaa aatggggact ggatggtgct acccctgtgc agtcccaaga acaagattca
     2461 tcaggaacag ttcggcagct ggtaagtcaa acaccaggag caatcagcta tttagctttc
     2521 tcatatctcg atgattccac acaagcacta agtatcgacg gtgtagaacc aaaagaagag
     2581 aatgtagcag ataacagctg gggaatctgg tcatatgaac atatgtacac gaacggaaaa
     2641 ccttcccctg aagtccaaaa attcttagac tacatgatga cagaagaaat ccaagaagga
     2701 cctgtcaaag aattgggcta tttaccaatc acaatgatgg aagtagaacg tgatcacgaa
     2761 ggaaatatca aataactagc agataaacat gccacggtag tcgttactat taaggaaaaa
     2821 tcaactatgt ttccccacat atacattttt cctttaattg tgaaaaatag ttcctcatat
     2881 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt attcgaaaaa
     2941 aagagctacg acatagaact gtcgtagctt tttttttgct atatgacagg aatgaaaggt
     3001 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
     3061 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
     3121 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
     3181 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
     3241 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
     3301 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
     3361 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
     3421 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
     3481 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
     3541 tattttagag actggacatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
     3601 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggcgatcc
     3661 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
     3721 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
     3781 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     3841 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     3901 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     3961 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     4021 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     4081 gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc tctaaaaaac aagaaataat
     4141 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     4201 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     4261 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     4321 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     4381 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     4441 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     4501 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     4561 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     4621 ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     4681 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     4741 attgctaaag cgtcgtaaca atacagtagc tatgacaggt gatggcgtga atgatattct
     4801 agccatgaaa gaagcagact gcagtatagc gatggcttcc ggaaatgaag ccgccatgca
     4861 ggcctcacaa gtggtcttac ttgattctga cttttcacgc atgccagaag ttgttgcaga
     4921 aggacggcgc gttgtgaaca atattgagcg atcagccagc ttgtttttag tgaagaatat
     4981 tttctccttt ttgctctcac tgttttcagt gatctttgct ctgacctatc ctttagaacc
     5041 atcacaaatc acattgatta gtttgtttac tatcggactt ccatctttct tgcttgctct
     5101 agaagaaaac aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg aaaaagctgt
     5161 gccaggtgga ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt
     5221 gaatctaaat aaaacagatg tttctacagc atctaccatg cttttgattg cagtagggtt
     5281 cctcgttctc tataaaatct gttcaccgct gaataaattc agaagtcaga tcattctttt
     5341 ctgtgctagc ggtaactttt tttcggtcat ctttttgcat aagctgtttt caatcaccgg
     5401 tatctcagct gtatcacttt tgctgttgag cattctattt ttctcggcag attcgatttt
     5461 taggcatttg accacattgg tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa
     5521 gccgtttagt cgcgtagtta aaaaatttct atcgatattt aaacgagaaa attgacgaaa
     5581 agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag
     5641 tcatccaact ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg
     5701 tgaaagtcga acctgttcca agatgactgt ctacagtaat cgtaccatga aggagttgca
     5761 cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc tgaatgccgg cttcgcgcct
     5821 tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac
     5881 caaagtcttg gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc
     5941 tgtcgctttt tgaatattgg acagcatttt caatcagatt tttgataatt ggataaagga
     6001 tatctgtttt agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct
     6061 tttcgatgat tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg
     6121 tctggatctc gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt
     6181 tatctaaccg gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt
     6241 catcttttgc accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt
     6301 ttaattcgtg agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt
     6361 tcgttaagtc atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc
     6421 gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg
     6481 gatgctcagg agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg
     6541 tgtaccgctg ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa
     6601 gcagtagtaa acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca
     6661 gtaacgtata taattgctct tctgtggtag tatacgcccg atacatctta ctcatttgtt
     6721 cgctcaacgc attgatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta
     6781 cttccgcctg attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg
     6841 gctggttttt ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca
     6901 gaaagaagaa aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg
     6961 gttctgcgat acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat
     7021 ataaaagatc cccattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat
     7081 tcaatacggc tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg
     7141 tgtcaaataa gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga
     7201 cgaattcttc caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc
     7261 ggatcagcag ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt
     7321 gaaaataatg ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa
     7381 agaagagacg ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc
     7441 aaatcctcta atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg
     7501 caaatgacta acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat
     7561 gcggtctagc aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag
     7621 taattcaaat tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt
     7681 ggtcaaatca gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga
     7741 ttgcttacga ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg
     7801 actaaatggt ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac
     7861 ttggtcatct ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg
     7921 ctgagtgatc tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa
     7981 tgattgttct aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta
     8041 gccttctttt tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac
     8101 tagtattttt ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat
     8161 agaatcagta caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt
     8221 gctgaataga aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc
     8281 ctgtcgctat gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg
     8341 agaagctgtc atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc
     8401 tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac
     8461 aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt
     8521 gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca
     8581 ttcgatttat gacgggagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc
     8641 ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg
     8701 tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga
     8761 ccttattgac agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat
     8821 taggtgagat cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc
     8881 ggacaagcaa gatcttggag gattatgaaa aaaaacttca tcttttttct aattatattg
     8941 ttcgagaaca ggtattggaa aatatgactg catatattac agataagtac ccggaagata
     9001 aaaaactact tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga
     9061 agaaaatatg gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag
     9121 aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc
     9181 cttactagaa gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga
     9241 acgtgcggta ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat
     9301 cgttgatgaa gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc
     9361 aaaaatacca actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac
     9421 tggtaaatta gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg
     9481 ggacgtatat cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt
     9541 tcctgtattt gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga
     9601 gttagcaaaa gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat
     9661 cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat
     9721 tttgcttgtt gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa
     9781 ccagcaatta ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg
     9841 ggcagcacta tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga
     9901 aaaattgact gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat
     9961 gggggatatg ctgacattga ttgaaaaagc gcagcaagac tatgacgaga aaaaagcgga
    10021 agaacttgca cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct
    10081 tgatcaagtg atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa
    10141 tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc
    10201 gatggtttat tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg
    10261 tcgtcgccga attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa
    10321 acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc
    10381 aggtatggac caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa
    10441 tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag
    10501 cttcggtaca gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt
    10561 tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca
    10621 aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca
    10681 aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat
    10741 tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatta aaaaagtcgc
    10801 aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg
    10861 aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat
    10921 cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta
    10981 tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct
    11041 aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt
    11101 cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg
    11161 cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg
    11221 ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca
    11281 aaccacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta
    11341 tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt
    11401 tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt
    11461 tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg
    11521 tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac
    11581 ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt
    11641 tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta
    11701 gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt
    11761 tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta
    11821 aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat
    11881 tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa
    11941 gtgattgtcg tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa
    12001 cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta
    12061 aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac
    12121 cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag
    12181 tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc
    12241 cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta
    12301 aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct
    12361 ataacaatct tacaaaaaat aagtaactgg agcaaataaa aacacttact gacaaaagct
    12421 acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt ctatttccta
    12481 aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact
    12541 gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat
    12601 ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc
    12661 acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga
    12721 ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca
    12781 accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga
    12841 agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta
    12901 acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg
    12961 aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga
    13021 aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca
    13081 ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac
    13141 tcgaattttc gagtgcagct tttttgtttt tttattaatt gaagaaatga ttatttggca
    13201 aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa
    13261 atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca
    13321 gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt
    13381 accaaaacgg ctaccaccac tgaacaaaca atggctacct caaataatga gaaaaagact
    13441 gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc
    13501 tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat
    13561 tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt
    13621 gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag
    13681 ccctttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat
    13741 cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga
    13801 atccgtggga ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct
    13861 tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg
    13921 ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg
    13981 atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga
    14041 aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat
    14101 agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact agaattgcct
    14161 cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata
    14221 cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc
    14281 aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgttaacac tattccaaga
    14341 cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt
    14401 attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt
    14461 aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat
    14521 catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc
    14581 accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc
    14641 atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat
    14701 tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg
    14761 aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca
    14821 atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat gattatccat
    14881 acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca
    14941 ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac
    15001 gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg
    15061 gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag
    15121 gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc
    15181 ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg
    15241 gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag
    15301 aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa
    15361 gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga
    15421 cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt
    15481 gactttttct aaaatattct ttacagtagg ttgataatat gttagtattt tatggtgagt
    15541 gcaaaagcac ttaactatta cgatattccg ctatgagaaa agctcataag aatatttgga
    15601 ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt
    15661 ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag
    15721 gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa
    15781 tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc
    15841 cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg
    15901 caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc
    15961 gttaatcatt gttatcagac tgagaaagaa cccttggtat tcaagggttc ttttttctta
    16021 gcatgtaatt tgaacaccct gatccagttt ttttctttaa ttattggtag gggagaataa
    16081 tggattagaa taaatctacc tataagtagt agtaaaaaaa agagcactat gataatatct
    16141 ttcttaagag taaccgcttt aataaaaaat ccagaatatt ctaacataaa tggagtattc
    16201 ttagaatcaa aaggagaaag aagatggttt atacttatac ggagcaagaa aaaatgaaag
    16261 tttgagacac attcatgaat gatggaaaag taaaacaagt accaagttca acaaaaaaga
    16321 aaaacatcct attgaaagaa gtcttgaaac gctttgacca tggagtgact tatacagaaa
    16381 cagaggttaa ttcgatactg ctgaacgtat tttctagtgg cgattatgtc gaacaaagac
    16441 gttatctaat cacatttggc tttttcaaaa gaagttcaga cggtcgggca tatcagatga
    16501 tgggaattga aaattagaag ttttaattta tactcattga aaaaagttca agtatgcgat
    16561 attggttcta caccgcatac ttgaactttt tttgtcatct gtatctataa tacaaagcca
    16621 agaacgttat tcaaaatatg gatagctatg ataaaagcta atgagcgatt cgcagttaaa
    16681 taagcaatcg tcagtaaaat cgttccagga gcgtacatga aaaaatcaat tggatgcgtt
    16741 acatgaataa agataattct cttttatttg tacgaataaa taccaaaacg tacctcctaa
    16801 aattagattc aaagaagata gcaaagagga aaaagatagt tctgcagcag ctatagcggc
    16861 atgtggattg ctagaacttt ccaaacaact gcctgtttca gatcctcgtc atttcgatta
    16921 cgaagagata gcaatcaaaa tcttggaaga attacagaaa aactatacaa cgaaacgagt
    16981 taacggatca aacggtctgc ttctccatgc cgtttatgat aaaaacagct taaaaggtgt
    17041 agatgaatgt gtgatctggg gcgattactt ttatgtagaa ggaatcactc gtctagctaa
    17101 aacatggtac tgttactggt gatgtataag agaagaggag gattcatata atgaagcgat
    17161 ggaaaaaaat gatgattgga tcaatagttt taggaatgtc actcgtattt ggtgcttgtg
    17221 gtaatcaaga agacagtagt gaagaatcag caaatgaaat taccgtttcg acatggaatt
    17281 atgaaacaac acctgaattc aaagcgctct ttgaagcatt cgaaaagaaa acgggaatca
    17341 aagtgaaagc agtagatatt gcttcagatg attatgatac gaaactcacc acgatgcttt
    17401 cttctggaga cacgacagat gtattgacga tgaaaaatct tttgtcgtat tcaaattatg
    17461 ctttacgaga tcagttagtc gatcaaacag aacgaattag agaattagac actgatgctg
    17521 ctgaagaaac atatgaaatg tatgatatcg atggaaaaac atatgcattg ccatatcgaa
    17581 cggacttttg ggtactatat tacaataaga aaatgtttga tgatgcagga atcgaatatc
    17641 caaaaaatct aacttgggac gaatatgaag atttagcaaa aaaacttagt aaaaatgacg
    17701 gacaagttta cggagcatat caacatatct ggcgttcgac aatccaagct attgcagctg
    17761 ctcaaaacga ggcgaatctt gttgaaccag attatcaata tatgactgat tattatgatc
    17821 gagcattgag gatgcaaaaa gaaggcgcgc agatggattt tggcacggct aaatcaacga
    17881 aagtaaccta tcagtctcaa tttgaagaac aaaaagcagc aatgatgtac atgggaactt
    17941 ggtatatggc tggcatactc gcaaacaaag aagcaagtaa gacagaagta gaatggggaa
    18001 ttacagctat tcctcagaag aaaaaaggag aatcggtcac gacgtttggc tcaccaacag
    18061 catttgctgt caataaaaac tcaaagaaac aaaaagcggc gcaagaattt attgaatttg
    18121 cagccagtga agaaggagca aaagtattag caggagtagg agtagttcct tcatatcgta
    18181 cggatgagat tgatcagctt tattttaatc tcgctggaat gccaacggat gaaatttcta
    18241 aaaaagcttt ttctcctgat gaaatcaaac ttgaatttcc gattgatacg tatgggcctg
    18301 caatcgataa gattttacaa gaagaatatg acttagtttt ggttggagat gaaacgccgg
    18361 aaaaaggaac ggtaaatatg gagaaacgtg tagctgcgga aaaagaataa tcatgaaagg
    18421 agagaagcca agagataaaa cactcttggt ttcgtcatcg aaatggagaa tagtataaat
    18481 aagaaagcta caaatcattt gcgtagaaaa cgaacgtgga tcgcttattc gtttatcgcc
    18541 ccgaatttca ttggtttttt cgtttttaca ttaataccaa tcctgttttc gcttgtacta
    18601 gcatttatga aatgggattc tttttcaacg ccagaatttg ctggactaga taactttcgt
    18661 aaaatggcca acgatgagac attttggatt tctttgaaaa atactttttt atataccgct
    18721 gctgtagtgc ctggcacact tgtttgttcg ttagggctag ctttactact aaatcagaaa
    18781 attagaggga tgaagctttt tcgtacgata tttttctttc cgtatgttac ttccttagta
    18841 gcaattgctg ttgtttggaa tatgttgttt catccaacag tgggacctat taatatcttt
    18901 ttaagacgat ttattgaaca tcctccaggt tggacttcaa gttcaaattg ggcgctttgg
    18961 gcaattgtaa ttgtcagtat ttggcgaggg atgggttatt acatgattct ataccttgca
    19021 ggactacaaa gtatccaaaa agaactatat gaagcgtctt cgctagatgg agcaaaccgt
    19081 tggaaacaat ttttgaatgt gacattacct tcgttgagac caacaacctt ctttgtaacg
    19141 atcatgcttg tcatcaattg tttcaaggtg tttgaccttg tgcaagtgat gacggatggc
    19201 ggccctggtc gagcgacaaa tgtgctggtt taccaaatct acaatgaagc attcaaaaaa
    19261 tttgattttg gttatgcttc agctatttca cttgttttgt tcgtaattgt tttgggcgtt
    19321 acggttgttc aattttcttg gaataagcgt cgtacttagg aggtgtgata tggaagagag
    19381 tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg
    19441 gttgggtatg attctgctga ttgtattagc tttattgacg atcattcctt ttttatggat
    19501 ggtttctgct tcattcaaag caaataatga agtgttaacg attccgattc aatggatccc
    19561 taaaacttgg catttagaga attatctggt tatttggtct cgtattcctt tagtgacatt
    19621 ttttaagaat acagcttttt taagcgttgt gatcacgttg attcagttgt ttacttctag
    19681 ttttgctgtt tacgggtttt ctgaactaaa ttttaaaggg cgcgatattt tatttttagc
    19741 atatatcggc acgattgctg tcccttggca gtcttatatg atcccgcagt tcattatgat
    19801 gagaaaagtg ggtttaacag atacgttgtg gtcgttggtg ctattacaag cgttcaatgc
    19861 ttttggtgtg ttcttgttga agcaatatta cagtagtatc ccagattctt tatgtgagag
    19921 tgcgcggatc gatggcttat cagagtggga aatctatcgc aagatcattt tacctcttac
    19981 aaaaccggcg attgccagtt tgacgatcat cacattcgtt aatacatgga acgattatat
    20041 ggggccattc atttatttgt cttctacgga aaataaaaca atccagttag ggttgaaaat
    20101 gtttattggg ctatttgatg cagagtacgc attaatcatg gctgcctcag ttgtttctgt
    20161 tcttcctgtt gcttttatat ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc
    20221 atcaggaatg aaggaatagg aggtgattat gaaagagcag cgttttgaca aaaatgttta
    20281 tatggaattc ttttattggt tgtatcaatc attggcaagt agcctttgtt tttggtgtgt
    20341 caatatcttg tatatagcag ctgcgttcct tttgtcgata gatggaagga atgtcttttc
    20401 ctttattttt tctttacttt ttattggtcc aggttgcatc gtgatgatag gtatgacaga
    20461 agaattagca aaagaaaaaa gtttttcacc agtacgtttg ttcataaaga cgtttcgcca
    20521 attttggcta aagggatttt tttattggct atttgcttgg atagttagtg tgataatgat
    20581 ttttgattgt ttctttttta tacgtttttc ttatggaaaa tggttgattc cgttgtttgt
    20641 tttacttgct tgttttactt gcttgtttat cagtttcctt tagcatcaac tgctggtatt
    20701 ttcaggtgag aaatccagcg tcgaaaccaa atcaagttct acggatagcg ttctactata
    20761 cgttgaaaaa atggtatgtg agtttactag attttttact attgacaagt ttgtttctct
    20821 tctttttcgt aaagccccaa tggtgtattc tactaggtcc ttctatcgta tttggtttga
    20881 tttacttcaa caatcgta
//
LOCUS       DAYYCM010000051        20641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000051 DAYYCM010000000
VERSION     DAYYCM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.052"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACHAY1_001928"
     CDS             112..306
                     /locus_tag="ACHAY1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593910.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACHAY1_001929"
     CDS             575..874
                     /locus_tag="ACHAY1_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593911.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACHAY1_001930"
     CDS             complement(971..1405)
                     /locus_tag="ACHAY1_001930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593912.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACHAY1_001931"
     CDS             complement(1637..2347)
                     /locus_tag="ACHAY1_001931"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8593913.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACHAY1_001932"
     CDS             complement(2337..3197)
                     /locus_tag="ACHAY1_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8593914.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACHAY1_001933"
     CDS             complement(3202..3840)
                     /locus_tag="ACHAY1_001933"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8593915.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACHAY1_001934"
     CDS             complement(3855..4154)
                     /locus_tag="ACHAY1_001934"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8593916.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACHAY1_001935"
     CDS             complement(4197..5681)
                     /locus_tag="ACHAY1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8593917.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACHAY1_001936"
     CDS             complement(5701..6162)
                     /locus_tag="ACHAY1_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8593918.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHAY1_001937"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHAY1_001937"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8593919.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACHAY1_001938"
     CDS             7465..8235
                     /locus_tag="ACHAY1_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8593920.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACHAY1_001939"
     CDS             complement(8508..9335)
                     /locus_tag="ACHAY1_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8593921.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACHAY1_001940"
     CDS             9903..11138
                     /locus_tag="ACHAY1_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8593922.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACHAY1_001941"
     CDS             complement(12137..12397)
                     /locus_tag="ACHAY1_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8593923.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACHAY1_001942"
     CDS             complement(12424..13725)
                     /locus_tag="ACHAY1_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8593924.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14017..14475)
                     /locus_tag="ACHAY1_001943"
     CDS             complement(14017..14475)
                     /locus_tag="ACHAY1_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8593925.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14488..15819)
                     /locus_tag="ACHAY1_001944"
     CDS             complement(14488..15819)
                     /locus_tag="ACHAY1_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8593926.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15830..17272)
                     /locus_tag="ACHAY1_001945"
     CDS             complement(15830..17272)
                     /locus_tag="ACHAY1_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8593927.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17259..18023)
                     /locus_tag="ACHAY1_001946"
     CDS             complement(17259..18023)
                     /locus_tag="ACHAY1_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8593928.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18127..19002)
                     /locus_tag="ACHAY1_001947"
     CDS             complement(18127..19002)
                     /locus_tag="ACHAY1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8593929.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19257..19341)
                     /locus_tag="ACHAY1_001948"
     tRNA            complement(19257..19341)
                     /locus_tag="ACHAY1_001948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19305..19307),aa:Leu,seq:caa)
     gene            complement(19359..19430)
                     /locus_tag="ACHAY1_001949"
     tRNA            complement(19359..19430)
                     /locus_tag="ACHAY1_001949"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19396..19398),aa:Gln,seq:ttg)
     gene            complement(19440..19512)
                     /locus_tag="ACHAY1_001950"
     tRNA            complement(19440..19512)
                     /locus_tag="ACHAY1_001950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19477..19479),aa:His,seq:gtg)
     gene            complement(19574..19658)
                     /locus_tag="ACHAY1_001951"
     tRNA            complement(19574..19658)
                     /locus_tag="ACHAY1_001951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19622..19624),aa:Leu,seq:caa)
     gene            complement(19679..19749)
                     /locus_tag="ACHAY1_001952"
     tRNA            complement(19679..19749)
                     /locus_tag="ACHAY1_001952"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19715..19717),aa:Cys,seq:gca)
     gene            complement(19795..19866)
                     /locus_tag="ACHAY1_001953"
     tRNA            complement(19795..19866)
                     /locus_tag="ACHAY1_001953"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19832..19834),aa:Gln,seq:ttg)
     gene            complement(19878..19950)
                     /locus_tag="ACHAY1_001954"
     tRNA            complement(19878..19950)
                     /locus_tag="ACHAY1_001954"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19915..19917),aa:His,seq:gtg)
     gene            complement(19953..20026)
                     /locus_tag="ACHAY1_001955"
     tRNA            complement(19953..20026)
                     /locus_tag="ACHAY1_001955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19992..19994),aa:Trp,seq:cca)
     gene            complement(20033..20113)
                     /locus_tag="ACHAY1_001956"
     tRNA            complement(20033..20113)
                     /locus_tag="ACHAY1_001956"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20077..20079),aa:Tyr,seq:gta)
     gene            complement(20115..20190)
                     /locus_tag="ACHAY1_001957"
     tRNA            complement(20115..20190)
                     /locus_tag="ACHAY1_001957"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20155..20157),aa:Phe,seq:gaa)
     gene            complement(20226..20298)
                     /locus_tag="ACHAY1_001958"
     tRNA            complement(20226..20298)
                     /locus_tag="ACHAY1_001958"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20263..20265),aa:Asp,seq:gtc)
     gene            complement(20308..20380)
                     /locus_tag="ACHAY1_001959"
     tRNA            complement(20308..20380)
                     /locus_tag="ACHAY1_001959"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20345..20347),aa:Val,seq:tac)
     gene            complement(20396..20467)
                     /locus_tag="ACHAY1_001960"
     tRNA            complement(20396..20467)
                     /locus_tag="ACHAY1_001960"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20432..20434),aa:Glu,seq:ttc)
     gene            complement(20482..20571)
                     /locus_tag="ACHAY1_001961"
     tRNA            complement(20482..20571)
                     /locus_tag="ACHAY1_001961"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20535..20537),aa:Ser,seq:gga)
BASE COUNT         6518 a         4325 c         3276 g         6522 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
    13921 aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc
    13981 ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt
    14041 aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac
    14101 aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag
    14161 tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag
    14221 ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt
    14281 tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg catggatata
    14341 tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc
    14401 ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt
    14461 tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac
    14521 tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa
    14581 tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt
    14641 ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag
    14701 tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga
    14761 ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc
    14821 attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt
    14881 caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc
    14941 tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat
    15001 atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt
    15061 gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc
    15121 gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa
    15181 ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat
    15241 ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat
    15301 atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat
    15361 cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc
    15421 gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca
    15481 cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc
    15541 caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg
    15601 atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac
    15661 aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa
    15721 tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc
    15781 gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta
    15841 ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat
    15901 ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga
    15961 ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga
    16021 tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac
    16081 ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt
    16141 cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca
    16201 aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat
    16261 tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt
    16321 aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa
    16381 gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac
    16441 cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct ttatagatag
    16501 ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc
    16561 cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag
    16621 gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa
    16681 aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa
    16741 cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa
    16801 aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa
    16861 aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta
    16921 ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta
    16981 agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct
    17041 tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa
    17101 cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca
    17161 tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct
    17221 gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact
    17281 aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg
    17341 acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac
    17401 gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc
    17461 aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca
    17521 tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc
    17581 cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa
    17641 gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt
    17701 tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga
    17761 taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt
    17821 ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt
    17881 agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt
    17941 cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc
    18001 gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa
    18061 ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc
    18121 cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat
    18181 ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt
    18241 gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata
    18301 aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc
    18361 tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc
    18421 tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat
    18481 agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc
    18541 gaccacttct actgctatat catattcatt tggataaagg aatctcattt ctggcaacat
    18601 aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt
    18661 caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat
    18721 cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg
    18781 tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg
    18841 cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc
    18901 gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc
    18961 tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa
    19021 acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt
    19081 gaatagtagc accctacata atttacaaaa atagtttaac acataataaa agcgttatca
    19141 atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca
    19201 taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga taattatacc
    19261 ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt ccagcgcgtc
    19321 tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg gattcgaacc
    19381 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaagg
    19441 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
    19501 cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac taagatgaag
    19561 acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg ggcactggat
    19621 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat tagtactaag
    19681 gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt accacttggc
    19741 tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata gaaactgggg
    19801 tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat
    19861 accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
    19921 ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac
    19981 actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag
    20041 agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact
    20101 tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat
    20161 ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag
    20221 aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
    20281 cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct
    20341 gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc
    20401 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
    20461 acgggccagg taaatttgct acggagagta agggattcga acccttgaga cagtttggca
    20521 ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa
    20581 gaagctcaat actctttctc aaaatgagaa ctccggcagt aggactcgaa cctacgacat
    20641 c
//
LOCUS       DAYYCM010000052        20197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000052 DAYYCM010000000
VERSION     DAYYCM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.053"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..715
                     /locus_tag="ACHAY1_001962"
     CDS             56..715
                     /locus_tag="ACHAY1_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8593930.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="ACHAY1_001963"
     CDS             800..1705
                     /locus_tag="ACHAY1_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8593931.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="ACHAY1_001964"
     CDS             complement(1707..2339)
                     /locus_tag="ACHAY1_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8593932.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="ACHAY1_001965"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="ACHAY1_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8593933.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="ACHAY1_001966"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="ACHAY1_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8593934.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="ACHAY1_001967"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="ACHAY1_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8593935.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4119..5456
                     /locus_tag="ACHAY1_001968"
     CDS             4119..5456
                     /locus_tag="ACHAY1_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8593936.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5504..7633
                     /locus_tag="ACHAY1_001969"
     CDS             5504..7633
                     /locus_tag="ACHAY1_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8593937.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7608..8297
                     /locus_tag="ACHAY1_001970"
     CDS             7608..8297
                     /locus_tag="ACHAY1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593938.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8705..9382)
                     /locus_tag="ACHAY1_001971"
     CDS             complement(8705..9382)
                     /locus_tag="ACHAY1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8593939.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(9518..9712)
                     /locus_tag="ACHAY1_001972"
     CDS             complement(9518..9712)
                     /locus_tag="ACHAY1_001972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593940.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9762..10202)
                     /locus_tag="ACHAY1_001973"
     CDS             complement(9762..10202)
                     /locus_tag="ACHAY1_001973"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8593941.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10206..11051)
                     /locus_tag="ACHAY1_001974"
     CDS             complement(10206..11051)
                     /locus_tag="ACHAY1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8593942.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11200..12618
                     /gene="pepV"
                     /locus_tag="ACHAY1_001975"
     CDS             11200..12618
                     /gene="pepV"
                     /locus_tag="ACHAY1_001975"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8593943.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12675..13622)
                     /locus_tag="ACHAY1_001976"
     CDS             complement(12675..13622)
                     /locus_tag="ACHAY1_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8593944.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13845..14195)
                     /locus_tag="ACHAY1_001977"
     CDS             complement(13845..14195)
                     /locus_tag="ACHAY1_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8593945.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14395..15474
                     /gene="pepA"
                     /locus_tag="ACHAY1_001978"
     CDS             14395..15474
                     /gene="pepA"
                     /locus_tag="ACHAY1_001978"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8593946.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLVEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15618..15938
                     /locus_tag="ACHAY1_001979"
     CDS             15618..15938
                     /locus_tag="ACHAY1_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8593947.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            15960..16424
                     /locus_tag="ACHAY1_001980"
     CDS             15960..16424
                     /locus_tag="ACHAY1_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8593948.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16629..17234
                     /gene="ytpR"
                     /locus_tag="ACHAY1_001981"
     CDS             16629..17234
                     /gene="ytpR"
                     /locus_tag="ACHAY1_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8593949.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17273..18562)
                     /locus_tag="ACHAY1_001982"
     CDS             complement(17273..18562)
                     /locus_tag="ACHAY1_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8593950.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            18990..19469
                     /gene="rlmH"
                     /locus_tag="ACHAY1_001983"
     CDS             18990..19469
                     /gene="rlmH"
                     /locus_tag="ACHAY1_001983"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8593951.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            19672..19797
                     /locus_tag="ACHAY1_001984"
     CDS             19672..19797
                     /locus_tag="ACHAY1_001984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593952.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            19794..>20197
                     /locus_tag="ACHAY1_001985"
     CDS             19794..>20197
                     /locus_tag="ACHAY1_001985"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8593953.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
BASE COUNT         6470 a         3670 c         4121 g         5936 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata gcataattaa ggttttttaa ttgaaaactg aacatttatg
     4021 gtcacccttt aatgaaagcg ctaactacat aagttagaat gagtttgact gatatattag
     4081 tttgcttatc agaaaaacaa aatggacgga ggaataacat gagtgatatt attattggta
     4141 ttttgttagt tatctcattc tttttcatgg tctggtactg tgtaaaaggc tacaacttga
     4201 tggtcggctt tgccatcatg gctactgtat ggatgggatt agcgctagtc ggaaacacgt
     4261 tttcaccaaa cccggcgatg gaagggcaag gagtaattga tgtgttgacg catatctata
     4321 cgacggggcc agctgagtac gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt
     4381 tcgggcgtgt tctagtagat agcggaatcg cagctaccct gattcgaaag gtagttgaac
     4441 ttggagggga caagccaaga atcacgatga gcttactatg tattgtaacc gctgttatat
     4501 tcatgtcgat gacaggaatc ggaccagtga tttctattgc agttattgtg ttgccaatct
     4561 tgatgtcatt aggtatttca gtaccggttg cactgttctc atttatgggc tcgattatgg
     4621 ctggaatctt tgctaatatc gtcaatttca aacaatatca aacgatttat gctggtttca
     4681 atccagcggc tgaaagctat acttataatg actatttcca aattggtatg attgggatga
     4741 ttgtaagctt agttgtggta ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct
     4801 atgcaatggc agcaaacgta ccagctgaag gtggcgacgc accaatgatc tcatggttag
     4861 ccgtattatt accagtttta ggggtcgttc ttttagatct tccaatcatc ttaggtttta
     4921 ttctagcagg tatctgggca ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa
     4981 tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt agatgttgcg ccaatggttg
     5041 gtttcttgat gaccttagcg atgttcaata attcagctgc gtatgcttct ccttattttt
     5101 cagctatttt tggagattgg ataccgcaat caccattagt cttggcaatc gtatttgcta
     5161 tattgacacc actaggattt ttccgcggtc caatgaatct agtcggttca ggctcagcta
     5221 ttttagctgt cgtattagct gttaatccaa cgatgtcgcc agcctttttg ttcccgttat
     5281 ttgcaatcac aacgattgct cctcagcatt tagatattac acaatcttgg gttgcttggg
     5341 gattaggtta tacaaaagta acttctcgag aatatatgaa gaaatccatc ccaactggtt
     5401 ggatcatcgg agcgatctta tgcctgatca cgttcttatt gtacgggaac gcctagtaaa
     5461 caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg
     5521 aatgggaatt gatgttggcg gtacacatac gaaagcagtc gcaatcgata atgcgacgca
     5581 cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg gagtagccgc
     5641 aggtgtcgtt cagtcttttc aaaattgttt gctggaaaat aatatcagtc cagaagatgt
     5701 tgtgtttgtt gcacattcta ccacacaagc gactaatgca ctgattgaag gagacgtagc
     5761 aaaagtcggc gtaatcggga tggcaaaagg tggattagaa ggttttttag caaaacgaca
     5821 aactagattg aacgatatcg atttagggaa taagaaaaaa atagagattg tcaatgcatt
     5881 cttacctgtc aaacacttaa atgtagaccg tgtttcagaa acgatttctt ctttagaaag
     5941 agaaagagcg gaggtcttgg tttcttctat ggcatttgga gtagataatg gggaaccaga
     6001 acgagttgtt tatgaagcag catcggtaaa atcgatccct acaacgatgg cttctgacat
     6061 cacaaaatta tatggattga caagaagaac tcggacagca gcaatcaatg catctatttt
     6121 accaaaaatg ctagatacgg caacctctac agaagattcc gtacgagaag caggggtcaa
     6181 tgtttctttg atgattatgc gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa
     6241 acgacctgtt ttaaccatgc tttccggtcc agcagcatca gtcatgggct ctttgatgta
     6301 cttgagagcg tctaatggtg tttattttga agtaggcgga acaacaacca atatcggggt
     6361 cattaaaaat ggacgtccag caatcgatta ttcaattgta ggtggccacc caacatatat
     6421 ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac gggctaatca
     6481 gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt gccggtctag attatgcagt
     6541 atttacggag acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg
     6601 agaccctgct gattacgtca aggtagtgat ggaagacgga gaagaagtga ctattacaaa
     6661 tacatgtgca gccaatgtcc ttggattagt gcaagaagaa catttttctt atggaaatgt
     6721 cccatctgca cgaaaagcca tccaagcatt agctgattat tgtcatacga cggttgaaga
     6781 catcgcagaa cagatcatgg aaaaatccta tgctaagatt gaaccggtga ttttagaatt
     6841 ggcagacaag tatcacttgg agaaagatca gatttctctt gtaggtgtag ggggcggcgc
     6901 tgcctcattg attacttact ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa
     6961 cgccgaagtg atttcttcta ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg
     7021 tatcatccct tcgccaagta aagaagatat acgatcgttg aaaaatgaag cgatgaataa
     7081 agcgattgaa tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa tcgatccaca
     7141 gacttcaaaa gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac
     7201 caaggagatc acaacagaag aagcactaga gcttgctgca gaggatatgc gattaaataa
     7261 aaatgaagtt tgcttattgg aaaatacacc tttcttttat gtatgcggcg aacagaatcg
     7321 ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa ggatttatca aggtccaacg
     7381 tggacatgct tcatgtatga aaacaactgc agccaattat atgacagctg tcgaacaact
     7441 ttgggaagat atggcagttt atcaaactga attgattgcc cgaccagaat tttatctctg
     7501 cttaggagca agagtctctg attttacagc gacggatctg gaacaactac agcttttgat
     7561 ggatctggaa gtatctacta tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat
     7621 caaacaaaca taagttagaa gagttactaa acgaattaca acagatcccc gaagaaattt
     7681 ggggattcta ccagtttcgg cgagatttat tttggaaaaa gattcctttg tccaagcaga
     7741 aaattcttat acaacaatca atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa
     7801 agtacccttt tgccgatgta ggagaaattt gtgaacaaat ggctgttcca atcgtttcct
     7861 gtgaatcaga gcaaataaac gaacgaatca cttttgcaac ttatgcagaa gatgaaggga
     7921 ttcgattgat gacggagccg ttagaaaaat taaaatgttc aggactagct tcgatctcta
     7981 aagaaacagc gcaagctttg attattggac atgaactatt tcaccacatc gaagcttctg
     8041 ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac
     8101 atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat gagtttttca aaagagatga
     8161 atcaaagcga tttttcgcct tatgtattag aggcagtctt actttggcca tataatgaaa
     8221 ttcgtagcca agggatctta gaagaaataa aaaaaataga aaaaagatgt acggagtatg
     8281 atttagatta caaataaaga gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa
     8341 taaacgatgt tctcctgtca actcctcggt cacaagtcaa aatattcaac aaacatgaga
     8401 agctgttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     8461 ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata
     8521 agaagctatt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     8581 ctcaataaac ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat
     8641 atgaggaact attttgccgt ttctttctct aatactcagg tcaaaacgct gattccaaaa
     8701 cctcttattt gatgggtctg tcctgaatat cttcaaaata atcggggtaa ttatgaatca
     8761 cgtattcttg ctcctcgata atcatatgta aatgcatcgt gcaaagaaat ccgacttcat
     8821 caaagttatg atcaatcatt gattgaagca ttgtctgatg ttcatctaat actcgacccc
     8881 agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg cgtactataa ctttctagcc
     8941 aatcccaaat ttctcgtttt ccagcaattt cataacaagt tcggtgaaaa tcagtatcca
     9001 aatcaaaaaa agctttttta ttttgagcga ttactgcctg atcggtttgt tctagtattt
     9061 tttctagttt agcttgatct tgcttggtca ttttcgcact gcattcttgc aggatcggtt
     9121 tttccaactt ttcccgcgta taacgggcta atgaagcaga tcggatatcg atttttgaaa
     9181 cataagtgcc ggactgtgga atggttttta caagctcttg attgcgcagt tggatcagtg
     9241 cttctcgaat cggtgtacgc ccgatagcta gttgttcttc taacacttta tcggagattt
     9301 tacttccagg tgctaactcg gaataaatga tttgtttacg tatagattca taagcttgct
     9361 gttgcaaatt attgataacc atttaacctt cctttctatg cagatataat ctattataac
     9421 ataaatcgct tacaaatcgt gtgggcataa aaacacagtc tgcaaaaaaa gccctgatag
     9481 gcatgcctat tagagcctcg aaaagaaaaa tattgaatca atctggtcgt acctcttgga
     9541 tcggtttgac acgaccgccg attcttttag caaaagcttc ggattctttt ttgtctttga
     9601 aaatgagcag attgtctaag tttttttccc gagtgcgaca gccagtttta tcgatgtaac
     9661 cgtgcattac tttaacgaca tacatgggga tcacctgctt ttttcttttc attatacgaa
     9721 ctactcgaca caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc
     9781 aaagaagcgt acgcttggaa taactgatct aactccggat tgactttgct cagcttcact
     9841 aatcgttgcg aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt
     9901 ttgtcttcag ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg
     9961 atagttggat ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt
    10021 acttccagaa cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga
    10081 tgtaccaata aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata
    10141 atccccattt gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc
    10201 atgactcatg cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt
    10261 gcttcgctga tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg
    10321 aaactatgaa gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa
    10381 aatcctgtga tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa
    10441 cctaaaggat tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa
    10501 acgagcacag caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt
    10561 atcggtagag aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg
    10621 ggatagggta gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc
    10681 agccattgtt cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa
    10741 gaagtaccca atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac
    10801 tggtcagtat ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc
    10861 cgattttttt catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa
    10921 gcactcataa tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca
    10981 aatgttcctt tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg
    11041 tttatcagca tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa
    11101 acagataaga aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat
    11161 catgaacacg aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa
    11221 gaagtagaag cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac
    11281 agcgagcgcg atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac
    11341 gcattgaaac atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat
    11401 aactatgcag ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac
    11461 atggatgtcg ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag
    11521 gatggaaaga tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac
    11581 tatgcgatga agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta
    11641 gtcggcagcg atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa
    11701 gaaccagatt ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga
    11761 aatgtttctt tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct
    11821 tttgtttccg gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta
    11881 ccaagtgcag atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat
    11941 ccagtaagcg gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa
    12001 ggcgcacatg gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc
    12061 ttagataact atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat
    12121 gtccatgaag atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc
    12181 aaattgacaa tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac
    12241 ttcatcaata tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac
    12301 atcgaacaaa cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa
    12361 ccacattacg taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac
    12421 cacactggag aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg
    12481 aaacgtggcg ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca
    12541 aacgaattta tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc
    12601 tatcgtttgg cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag
    12661 tcttgatcct ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat
    12721 tcatattctt tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa
    12781 agaagtgcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag
    12841 cttgtcagca tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag
    12901 aagatggtcc aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc
    12961 atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga
    13021 cgatcacgat aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc
    13081 gtcaattccg tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata
    13141 cgattgaatc ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta
    13201 attgtatttc taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag
    13261 taggttccat ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc
    13321 tcgagcaaac catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa
    13381 tagtcggcat cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt
    13441 tcttttttta cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta
    13501 ttatcataga cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt
    13561 gaaattgttg cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc
    13621 atttttcact ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa
    13681 aggaaatgct agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata
    13741 tgtttggaaa gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct
    13801 tttgaatagt gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct
    13861 ttttttcttg aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa
    13921 acagtttcgc catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg
    13981 aatttcctaa taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt
    14041 agaaaagatg acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga
    14101 taccaaatcg ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg
    14161 cctttaaaat agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact
    14221 tgttactcct tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct
    14281 accagaaaaa ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc
    14341 gcagatcaat ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa
    14401 gaaaaaacat ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa
    14461 caagatgttc gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta
    14521 gatggtttgg gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta
    14581 atggtcgcag cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg
    14641 ctttttaaag tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt
    14701 acacttaaaa cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat
    14761 ttattgcgag gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct
    14821 gggtttgaat caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt
    14881 ccttacgctg aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac
    14941 aaccgctatg gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta
    15001 ggtcatacat tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa
    15061 gcttctgtca ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat
    15121 gatacagtga cgaaaaatgg cacattcgga catttagtag aaggtacatt gatgcgtatc
    15181 caagatccag gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa
    15241 acaaacaata tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct
    15301 catacacaaa acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat
    15361 acgcatcaaa cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa
    15421 acactgaaag gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat
    15481 agtgaaacta taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag
    15541 gatcaatgtt tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag
    15601 aaagtagagg acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag
    15661 aaactggaaa gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca
    15721 aaccgcaaat gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc
    15781 gtgacaagta catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg
    15841 tgatccaaga cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg
    15901 aaatcgaatc gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga
    15961 tgaatcaaga atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg
    16021 ctttcaaaaa agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg
    16081 taatcgatca gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg
    16141 atcagcaaac tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg
    16201 ttgattacac aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag
    16261 tcaaattgcc aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg
    16321 cagtcgaacg ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg
    16381 atgtcttgat cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta
    16441 cgaccttttt aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag
    16501 tcccttgtta cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa
    16561 aaatttcatg cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag
    16621 gaaagaacat gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta
    16681 tagccgacga taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca
    16741 aagatgaaca aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa
    16801 tcgaagggaa cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga
    16861 tcaaagaagc aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg
    16921 ggtacgtaaa aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag
    16981 aagtagacaa tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac
    17041 aaaaagttgt cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag
    17101 gaactttgcg aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc
    17161 caaatgctcc agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag
    17221 catttccaaa ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt
    17281 cactattttg attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt
    17341 tttctttcga gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca
    17401 aagcagattg aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt
    17461 cgtattgttt caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga
    17521 caccgttagt tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg
    17581 cagataagtc gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca
    17641 gttgattgat gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc
    17701 ttacattaga agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt
    17761 gtccttcgat attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt
    17821 ggatcgcatt gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca
    17881 aggaagaaat gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa
    17941 tcgcaatcgc aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt
    18001 cgactttgtc tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta
    18061 attcagcttt taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta
    18121 ctacgtgatt attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc
    18181 ttccttcact ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc
    18241 cgaatagtcc gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga
    18301 cagcgtcttg aactttatct accgctttgg tgatatcaga atctacattg actttgacat
    18361 tttccacaac tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt
    18421 tgccagtgcc catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc
    18481 caacaagccc cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg
    18541 gtgtcacatt ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta
    18601 gtatagtctt atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact
    18661 ttaggaaaaa tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt
    18721 gtaaaactac gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta
    18781 taaagtggat aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta
    18841 aaataacact gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc
    18901 tttttaacaa aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa
    18961 gtgagcatga agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat
    19021 taaaagaaaa atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg
    19081 caaaaatcga attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc
    19141 aaatgagaca agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag
    19201 aatacgtcta tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac
    19261 aaatcgacca attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt
    19321 taggactcag cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga
    19381 cgtatccaca tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga
    19441 tcaatgcggg ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta
    19501 ttttaaataa tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc
    19561 tttggtagcc aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca
    19621 tagaaacaaa tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat
    19681 gaaaaaaagt gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta
    19741 aatggactgg gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac
    19801 atagtaattt tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt
    19861 tttttaaaat gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca
    19921 attattttga tgctttactt aatagctatg aagatttaat tgtttacaga gaaaataaga
    19981 atattgatat tttattggta tcggaaaaaa ataaaatagt ggtgtgcatc gaaaataaaa
    20041 ttctgtcttc ggagtctgga gggcaactta ataaatacca aatatatata gaacgcaatt
    20101 attctaacta taaaaaaatt tttgtttttt taactccaga tggaaatgaa cccacaaatc
    20161 ctagttgggg cataattact tacaagaata tcgttga
//
LOCUS       DAYYCM010000053        19631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000053 DAYYCM010000000
VERSION     DAYYCM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.054"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAY1_001986"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHAY1_001986"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8593954.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHAY1_001987"
     CDS             complement(1174..1869)
                     /locus_tag="ACHAY1_001987"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8593955.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHAY1_001988"
     CDS             complement(1882..2598)
                     /locus_tag="ACHAY1_001988"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8593956.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHAY1_001989"
     CDS             2928..3536
                     /locus_tag="ACHAY1_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8593957.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHAY1_001990"
     CDS             3861..4184
                     /locus_tag="ACHAY1_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8593958.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHAY1_001991"
     CDS             4366..5736
                     /locus_tag="ACHAY1_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8593959.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHAY1_001992"
     CDS             complement(5866..7167)
                     /locus_tag="ACHAY1_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8593960.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHAY1_001993"
     CDS             complement(7521..9512)
                     /locus_tag="ACHAY1_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8593961.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHAY1_001994"
     CDS             complement(9656..9922)
                     /locus_tag="ACHAY1_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8593962.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAY1_001995"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHAY1_001995"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8593963.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAY1_001996"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHAY1_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8593964.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHAY1_001997"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHAY1_001997"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8593965.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHAY1_001998"
     CDS             complement(14082..14603)
                     /locus_tag="ACHAY1_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8593966.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHAY1_001999"
     CDS             complement(14606..15448)
                     /locus_tag="ACHAY1_001999"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8593967.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHAY1_002000"
     CDS             complement(15441..16166)
                     /locus_tag="ACHAY1_002000"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8593968.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHAY1_002001"
     CDS             16342..16638
                     /locus_tag="ACHAY1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593969.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHAY1_002002"
     CDS             17206..18798
                     /locus_tag="ACHAY1_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8593970.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHAY1_002003"
     CDS             18811..19551
                     /locus_tag="ACHAY1_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8593971.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAYYCM010000054        19404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000054 DAYYCM010000000
VERSION     DAYYCM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.055"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..635)
                     /locus_tag="ACHAY1_002004"
     CDS             complement(39..635)
                     /locus_tag="ACHAY1_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8593972.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="ACHAY1_002005"
     CDS             985..1329
                     /locus_tag="ACHAY1_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8593973.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="ACHAY1_002006"
     CDS             1330..1749
                     /locus_tag="ACHAY1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8593974.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="ACHAY1_002007"
     CDS             complement(1841..2191)
                     /locus_tag="ACHAY1_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8593975.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="ACHAY1_002008"
     CDS             complement(2157..3488)
                     /locus_tag="ACHAY1_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8593976.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="ACHAY1_002009"
     CDS             3743..4309
                     /locus_tag="ACHAY1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8593977.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="ACHAY1_002010"
     tRNA            4454..4541
                     /locus_tag="ACHAY1_002010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="ACHAY1_002011"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="ACHAY1_002011"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8593978.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="ACHAY1_002012"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="ACHAY1_002012"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8593979.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="ACHAY1_002013"
     CDS             complement(8266..8712)
                     /locus_tag="ACHAY1_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8593980.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8940..9089
                     /gene="rpmG"
                     /locus_tag="ACHAY1_002014"
     CDS             8940..9089
                     /gene="rpmG"
                     /locus_tag="ACHAY1_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8593981.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9177..9695
                     /locus_tag="ACHAY1_002015"
     CDS             9177..9695
                     /locus_tag="ACHAY1_002015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8593982.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9772..10728)
                     /locus_tag="ACHAY1_002016"
     CDS             complement(9772..10728)
                     /locus_tag="ACHAY1_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8593983.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10894..11700
                     /locus_tag="ACHAY1_002017"
     CDS             10894..11700
                     /locus_tag="ACHAY1_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8593984.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11697..12686
                     /locus_tag="ACHAY1_002018"
     CDS             11697..12686
                     /locus_tag="ACHAY1_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8593985.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12683..13687
                     /locus_tag="ACHAY1_002019"
     CDS             12683..13687
                     /locus_tag="ACHAY1_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8593986.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13819..14361
                     /locus_tag="ACHAY1_002020"
     CDS             13819..14361
                     /locus_tag="ACHAY1_002020"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8593987.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14381..15079
                     /locus_tag="ACHAY1_002021"
     CDS             14381..15079
                     /locus_tag="ACHAY1_002021"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8593988.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15100..15312
                     /locus_tag="ACHAY1_002022"
     CDS             15100..15312
                     /locus_tag="ACHAY1_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8593989.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15312..16274
                     /locus_tag="ACHAY1_002023"
     CDS             15312..16274
                     /locus_tag="ACHAY1_002023"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8593990.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16292..16687
                     /locus_tag="ACHAY1_002024"
     CDS             16292..16687
                     /locus_tag="ACHAY1_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8593991.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16850..17215
                     /locus_tag="ACHAY1_002025"
     CDS             16850..17215
                     /locus_tag="ACHAY1_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8593992.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17212..19023
                     /locus_tag="ACHAY1_002026"
     CDS             17212..19023
                     /locus_tag="ACHAY1_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8593993.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19085..19252)
                     /locus_tag="ACHAY1_002027"
     CDS             complement(19085..19252)
                     /locus_tag="ACHAY1_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8593994.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6282 a         3299 c         3945 g         5878 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taaaaaacat aatctatata aacaagtgta
     8761 agggtattta tacaaaaaga aaaagatttt gtttgagaag taaaaccgtt gtatttatcg
     8821 tcaagtagtt ttacttaaca aaatcgtgaa aaacactagc aatctagaaa aaatcatggt
     8881 aagataatca agtctgagag tcatcttaga ggattcttgt aaaaatggga gggaaaaaca
     8941 tgcgcgtaaa catcacttta gagtgtactt cttgtaaaga acgtaactat ctaacaagca
     9001 aaaacaaacg taacaatcct gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca
     9061 aagttacttt gcaccgtgaa acaaaataat gtttttaaag ttctcaattc cttgctggtc
     9121 aaagaattga gacttttttt atctcaaaac atctaaaatc ataaggagcg gataatatgt
     9181 ttatttccat gtgggcacaa gataaaaatg gtctgatcgg caaaaatggt ttgttaccat
     9241 ggcgattgcc aaatgatatg cgtttctttc gcgaacatac aatggatcga attttagtga
     9301 tgggaagaaa gacctatgaa ggaatggggg atctttcact tccttatcgc catatcatcg
     9361 tgttgacaac gcaaccaaat tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg
     9421 atgaactttt gtcgtatagt caaacgatct cagaagatat ctatgtttcg ggtggtagtc
     9481 ggatttttca agaacttctt ccttacactg gtcggatctg gcgaacattg atcgatagca
     9541 cgtttgaagg cgatacgtat attgggaata ttgatttttc agacttcgcc cttatagagg
     9601 agtatgaggg gataacggat caagaaaatc tatatgccca tcgttttcaa aaatgggaaa
     9661 gagtaaaaaa tagggttgct tcaaaagaag aataaaatct atgcgtctag tttgttaata
     9721 agaaaaaaag catctcacga ctaatcgtga agattctttt tttagttaaa atcaatttag
     9781 tttttctttg atatcattga ccatttcctg actggcaata ggtccgttat atagccaact
     9841 agattcaggt ccgaattgca gtacatgatt gtctgtcact gctttaagat tcttccagct
     9901 tggttcattg aacatggcag catctttgtc actgttcaca aggatgatgt agtcagcatc
     9961 taattcagct agcttttcag aggaaacagc agaccagtca gaagtggctt ttttggagac
    10021 ctcttccact agatcaggaa caccaaattc cagatcgtcg tatacgattc ttccacttga
    10081 ccgatttttg ctgaccataa aggctgagtt gttggtcacc cacaacactg cagctgtctt
    10141 gttctcgatt ttatctgcta gttcttctcg agttttcttg atggattttt gataatcgga
    10201 aatgattttt tctgcctctt tttcttttgc caaagctttg ccgacatctt ttagttgttc
    10261 ttcccatgtg acatctgttc cgttttttac aacataagtt ggcgcgattt tgctatattg
    10321 ttcgtatttc ccgccttcga cagtagcgga agaagaaatc aacagcaaat caggctcaaa
    10381 gcttaacact ttctcataag gaaggtcata agaaatcgtt gggacatcct ttaaatcatc
    10441 ttgcagataa tcttgaatgg aaccactgcc aacagtccac tgggcgatag gtttttcccc
    10501 tagtgcaatc agatagtctt ctaagtaact gccgatgatc cgtttgggcg cgtttggaat
    10561 ctctacttta tgtccaagtg tatctgtgac tgtatgagta ctattggatg aagtcgtcga
    10621 attggcagtt gtagctgttt ctttatctga agagggggag caagcgctca gtactccagc
    10681 cagcagcaat atgctagata atgtgaatac ttttgttttt aatttcattt ctttcctcct
    10741 aacataattg agaatctttc tcattggtta ttattcctga tatttcgaac gctgtcaacc
    10801 gcttcatttg gctatagata tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa
    10861 ttgagaatga ttttcagcga aaggatgggt gaaatgacga gattaagagc agctgacatt
    10921 gcagccggct acgaaaacaa aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg
    10981 aaaatcacta gtttgattgg gccaaatgga agtggaaaat ctactttatt gaaaagtttt
    11041 acccgtatac tacctgtgaa atcaggaaag atcattgtca ataactctga tatctctaca
    11101 tacagttcaa aagaacttgc aaaaaaaatt acattattag cacaaactag tgaacaccct
    11161 acaggtatga cagtagaaga agtagtatct tacggacgat acccttatca aaaactgttc
    11221 agtggcatgg acacgcaaga tcatgaagca atcgagtggg cgttggatgc tacaggtcta
    11281 gctggatttc gtatgcggga tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg
    11341 atcgctatgg cgcttgctca ggaaacagat atcttgatct tggatgaacc gacaacattt
    11401 ttagatccgg cacaccaatt agagatccta cagttattga ctgaaatcaa tcaaatgaaa
    11461 caaacaacga tcttgatgtc gattcatgac attaatcatg cttcacgttt ttcagattat
    11521 ttattagcaa tgaaaaacgg taagttgatc acagaaggta cacctgaaga agtgatcaca
    11581 aaagagtgta tgaaaaaaat atttgagatt gatgcaacga ttgtcacgct tccaagaaca
    11641 aataaaccag tttttctaac ctatgatttg ttgccaaaag agcaggagga tcaattatga
    11701 ggactatgaa gaaaaataga ctcctgcttt tggtctgttt tgttcttttg attatttttt
    11761 ttgttgctct ttcaaatggc gcagcgactg tgggaaaagc agatttttgg caggcgttgt
    11821 ttgattttga tagatcaaaa caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg
    11881 tggcgggagc atttcttgta gggagttgtt ttgcattgag cggtgcattg atgcaaggag
    11941 tgacaagaaa tcctttagca gattcaggct tactaggaat caacgcgggt gcttcgttgg
    12001 caatggccct tagttttgct ttttggccta aagcttctga tttttctgtg tttctaatgt
    12061 cattggcagg ttcttttgct gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa
    12121 tgggcatgaa tcctttacag ctgattttag ctggcgtagc tattagttct ttttttactg
    12181 ctatcagcca agcacttacc caattcttta atctgcagca ggatcttgct ttttggtttg
    12241 taggcggagc tgccaatatc acatggaaac aattagcagt attgtttcct ttatatatac
    12301 ttgctgtttt ggtttctttt tcattaggaa aagcaatatc tttgattcat ttgagtgaaa
    12361 cacatgcatt agctcttgga agtcatccta aacgaacgcg gttgattgtt ttctgtctag
    12421 ttgcagtgtt agcagccatt gccgtttcgt tagtcggacc tgtttcattt attgggctgt
    12481 tggttccgcc agttgttcgc ggaatggttg gaacggacta tcgttatgtt ctacctgtct
    12541 cttttttagt tggcggtgga ttggttatgt tggcggattt tacagcgagg atgatcaatc
    12601 ccccttttga aacacctttt ggattagtca ttgcattgat tggcgttccg tttttactga
    12661 tacaagtaag gagggaacaa cggtgaaaaa aatatattgg acgttctatt tcttgctttt
    12721 tgtacttgtc atcctgtcta taggtagttt aatgattggt acgcctttta tttacctgga
    12781 tcagcttttt gccacgttca tagggaaaga atcatcttct ttggcgctga ttgttagaga
    12841 atttcgagtt cctcgattac tgatcagttt attagcagga gcttgtcttg gcgtttctgg
    12901 ttatatcttg caaggagtaa caagaaatga attggcagat tctagtatat tgggcattaa
    12961 tgccggagca ggatttttag tgatgcttta tttaggtttc ttttcgcact attcgctgcc
    13021 gtttctattg ctagttgtag cctttctagg tggtgtttta gctgcttgct gtgtctatct
    13081 tgcggcctat gatcgaaatg gctttttggg tatgaatcga atcttattgt ccggtattgc
    13141 cgttaatgcc ggcctttctg cattaacatt gctatgcacg atccaattat ctaaggaaaa
    13201 ttatggattt gtgaatgcat ggttagcggg atcaatctgg ggtgcgagct ggccttatgt
    13261 ttgggcattg cttccatggg cggttatctt aattccgcta ggaggacttt atgcacagag
    13321 gattggattg ctatcatttg gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa
    13381 aagtcaaaaa atccttttgt tgttagctgt tgctttggca agcggtagtg tagcggtgac
    13441 aggtagttta tcttttgtag gactactagc tcctcatgct gcgaagctag tcgtacgtaa
    13501 agaaaataac tggacctttg ttctgagcgg tttgttcggt tccatttttc tgcttagtgc
    13561 agatatggtt gctagagtga ttctaccaag tggggaagta cctacaggaa ttttgattgc
    13621 cctttttggt gcgccatatt ttctgtatgc actttttatc agacaaaaac acgcgctgaa
    13681 cagttgaaat ttattaaaaa aggaaggcca atcatttcta ggtcttcttt ttattctatt
    13741 tatgcattta tttgaaagtt cgattgctat ttttcttttt ttcctatacc attttatgag
    13801 agaaggaagg gtgagttttt ggaccaattg aaagtaagat tacgccggaa aggaatcgaa
    13861 aagctgaaaa atctggccaa aagtggcaag aaaaaggaaa aagaacagcg aatctataaa
    13921 cagctattcg ccagcgagta ctggaagtca gcaaaagtga tagggacgac tttatcgaat
    13981 gaatttgaac tggatacaca gccggttatc caacgagcag aaaaagaaca aaagaaaata
    14041 gttgtaccta aaactttgcc gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc
    14101 gttcttgtac gttcgagttt tggtgttctt gagccacaat cagagaagct ctatttgccg
    14161 gagatgatcg atctcctcat tgtaccaggt attatctaca atagagccgg ttatcgaatc
    14221 ggttttggcg gtggttatta cgaccggtat ttaaataatt ttcataaaaa aacatgtagc
    14281 cttgtttttc gtgaacaatt gaatgaacaa tggcaaccgg agagctttga tcagaagatc
    14341 caacaattgt ttattgacta aaatgaaatg ggaggttttc atgaattatc aacagcaaat
    14401 aaaattgcgt gcatggttaa gacgaccgtt atggacctat gcttttttag ggatacagac
    14461 agcggtcttc ctcatgatgg aattatttcc gcgacttgag atcccttatt atgccggaat
    14521 gtatggtcct tatcttgttc acttcaatga atggtggcgt ttagtgacac caatcttcat
    14581 ccattttgga ctgatgcatt tcgtaatgaa ctcactgata ctgtatttta tggggcagca
    14641 aatcgaggca atctatggcc attggcgttt tttcttgatt tatatgttca gcgggataat
    14701 gggaaataca gcaagctttg cttttaacga agcgaatgtt ctgtctggtg gtgcgagtac
    14761 ttcgatattc ggtttgttcg gtgcgttgtt tatcttaggg tttcatttta aacataaccc
    14821 agcgatccag cagctagtca gacattttct tttatttatt gtgttgacat ttgtttttgg
    14881 tcttctggat acctctgtag atgtttgggg acatgtcggt gggctaattg gcggattagt
    14941 gttagggaat gttttgggtc taccgaaaca acagacaagc tattcgatac accaaaggat
    15001 tctttctaca ctagtttttg tatttctttt tgtaatatgt attttactgg gtttaaaaaa
    15061 atatggttta cttgtataat atgaaggaag tgcttaaaga tggagagctt atatgatgta
    15121 cagcagctgt tcaaaaaatt tggcatcttt atctatgtgg gtgcacgcat ctatgacatt
    15181 gaattaatga ctatcgaatt gcgtaatctg tatgatagcc gtttgattga tcatgataca
    15241 tacatgacag cgtggcgcat attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa
    15301 ggaaatttgt aatggacaag aagattattg ggattgattt aggtggaaca acagcaaaat
    15361 ttgcaatttt aacaccagaa ggagagattc aacaaaaatg gagcatcgat accaatattt
    15421 tggatgatgg taagcatatc gttcctgaaa tcattgaatc aatcaaccat cgtttgaatt
    15481 tatataatat gaaagccgaa gactttatcg gaatcggcat gggtacccca ggaagcgtgg
    15541 acagcgaagc tggaacggtt attggtgcgt ataacttaaa ctggacagaa gttcaatttg
    15601 tgaaaaaact gatcgaggca ggaacaggaa tcaaattcgc tatcgataat gatgcgaatg
    15661 ttgctgcttt aggtgaacga tggaaaggtg ctggagaaaa cgatccagac gttgtattcg
    15721 ttactctagg aacaggtgtt ggcggtggta tcgttgctgg cggaaatttg attcacggcg
    15781 tgagcggagc aggcggagag atcggccata tcactgtgga tcctgaaggg ttcgaatgta
    15841 cttgcggtaa acgcggatgt ttagaaactg tgtctagcgc tactggtgtg gtacgtctag
    15901 gtcgttactt ggcagaagaa tacgcaggag attcaaaact gaaagcaatg ttagacaacg
    15961 gtgaagaagt aacaagtaaa gatatctttg aaatggctca agaagacgat ccgtttgcat
    16021 tgatggttgt tgatcgcgta tgcttctact taggtttagc ttgcgccaac ttggggaata
    16081 cattgaatcc gtcaagtatt gtgttaggtg gaggagtatc ggcagcagga gaatttttac
    16141 gtagccgtgt agagaaatat tttaaagaat ttactttccc gcaagtaaga gaatcaacaa
    16201 aaatcaaatt agcagagctt ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct
    16261 tgaaatttgc ttaaaaaaac ggaggcatta catggtttta tgggtaatta atatcatttt
    16321 gctattgatt gtgcttgcaa tcatcttttg ggaagtctat ctacgagtaa tggccaagcg
    16381 ttcagcaacg actttgacag aagaagaatt tcgagaagga atgagaaaag cacaagtaat
    16441 cgatgttcgc gaaaaagatg tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc
    16501 ttacactgtt ttgaaagatt cattaggatc tattcgaaaa gaccagcctg tctatattta
    16561 cgatcaaaag aaaagcttga gcatccgtac agcaaataaa ttacgtaaag caggttatca
    16621 agagatttat ttacttaagg gtggatatga cggatggtct ggaaaaatca aaagtaaaaa
    16681 agtataaaac agatgattgt atgacagctt taaaaaccga atcaatcatt agattttgac
    16741 caagatcaca tgaacggcgc aatggatagt tgtgccgttc atttttttta ttttgtgcta
    16801 ttatctacat atataagcta agcgaaaagg cagaaagaag gaaacaacta tgatcaaatt
    16861 aaaacgtgtt taccttgatg ctgaaaaaga ggatggctat agaatactcg tggataggtt
    16921 ctggccacga ggaatttcaa aagaaaaagc gcagttagat ctgtggttga aagaaattgc
    16981 acctagtaat gaagcacgca aaacttttga ccatgtacca gaacgatttg cttcatttgc
    17041 aaagaaatac cagcaagaat tgattttcga agaagaaaag aagcaagcta tttttaaact
    17101 cagtcaactt atgaaagaac atccagtcat tacttttgtt tatggtgcaa aaaatgaaca
    17161 tcagaaccat gctgttttat tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata
    17221 gggatcattg gtgccggacc tagaggatta agtatagcag aaaggattct ccataataat
    17281 caggacagac attctcttga actttttctt ttcgatccca atggtcctgg aggtcgagtt
    17341 tggcggttga atcagccacc ggagttatta atgaattctg tttctcaaca agtgacgctt
    17401 tttacagatg aaacattgtc cagtggagga ttggtaacac agggacccaa cttgtttcaa
    17461 tggagtaaag gacctgctac agattatatc gaacagacgg taaccaaaaa taagcaacta
    17521 attatagaag aaatcaatcg tctggaaaaa gatcagcaat ctactcgatg tttatacgga
    17581 ctctatcaac gctggttttt ctcaaagttg caagaagaat ttccagcttc tattcagttt
    17641 gtttattctt tagtaaaaaa agtcagcaaa agaaaagatg gttatctttt acagacagat
    17701 cagcaaagct attctatcga tcagcttgtt ttagcaagcg gtcactggga aaatgatgag
    17761 acagaagaag aaagagtgtg ggtagactac gcaaagaaaa atgatttact gtatcagctt
    17821 ccttctaatc ctgcagatgt cgatctttcg aagataccgc ctaaagaaaa tgtcttttta
    17881 agaggtctag gcttaagttt ttttgattat gtcggattgt ttacagtatc aagagaaggg
    17941 cgcttcgaga acgaaaaagg aaaacttgtc tatcatccat caggcaagga accgattgta
    18001 tacagtggat caagaagggg gctcccttat taccctcgag gaagaaacca aaagcaaggc
    18061 ggatcaatgg catggccacg gttactaaca aaagaaaacc tggagcaatg gcatcagtcg
    18121 ggatttttga ctggcgaaac attttttgac tatctgaaaa aagacgttga gttgttttat
    18181 tacaagaaaa tcatcgaaga atatcatttg ccgattccaa agaaaaattt tgagcatgat
    18241 tttctctccc tctcttcaga ggagtgtctc ggaaagcatc cagcactaat cccttataga
    18301 tgggattggt cgtatttaga aattcctctt acctatcagg atgagtcctt tgcagaagct
    18361 agccgagcat ttattgaagg gcagataaaa gaagcggaaa aaggcaattg cacaggagca
    18421 tttgcttcaa catttgatgc attaaaagac tggcgtgatc caatccggca ggctattgaa
    18481 tggcacgttt tttctgcaaa ggaatacaaa gaaatactat ggggatggtt tactcatcta
    18541 aatagctttt taacgatcgg tccgccagtc attcggacga aagaattagc agcactgata
    18601 gatgcgggta tttttcattt gttcgaaccg ccatttgata ttcagcaaaa gcacaaccgc
    18661 tatgttattt ctctacaaga taaagaaatc agcagtcgct atttgattga agctcgattg
    18721 ccacaaacaa gcttttctcg ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa
    18781 cttgttcgcc ctttttcatt ttcagataat acaggaagtt atcattcaga agctatagat
    18841 gttgagcgag gtacgaatcg cgtgattgat gcttcagggg aaatccagtc tgacttttat
    18901 tgctatggaa tacctgttga aggtgtggat tggctaactg cagcagtagc tagaccttat
    18961 acagatccat ggaatctgag acaggcagat aaaatagcac aacttatatt gactgacaaa
    19021 taaaaatacg catgatgact tatctgttct ttcacaagtc ttcatgcgtg tttttaaata
    19081 attattaacg agaaacgcgt ttgcgcattg cttttttacg gttttcttcg atcattgctt
    19141 ctttttcgtc aattggatcg atcactgttt ttttcacaga agcgaccaaa ccagcaacgg
    19201 cagcggctgt tgccagactt cctactaata ttccagatac aaattttttc attgaatcca
    19261 cctcatttat tatttgttat atctattatg gaacaaaaga gaaaaaaagg ctagttttac
    19321 gtttgtttgt aaacctttgc aaaaaatcct tgaaaatgtt tactaaatgt tttattataa
    19381 atttaccata tgaatttacg aaat
//
LOCUS       DAYYCM010000055        18367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000055 DAYYCM010000000
VERSION     DAYYCM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.056"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..429)
                     /locus_tag="ACHAY1_002028"
     tRNA            complement(346..429)
                     /locus_tag="ACHAY1_002028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(393..395),aa:Leu,seq:cag)
     gene            957..1457
                     /locus_tag="ACHAY1_002029"
     CDS             957..1457
                     /locus_tag="ACHAY1_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8593995.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1647..1991)
                     /locus_tag="ACHAY1_002030"
     CDS             complement(1647..1991)
                     /locus_tag="ACHAY1_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8593996.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2145..3065)
                     /locus_tag="ACHAY1_002031"
     CDS             complement(2145..3065)
                     /locus_tag="ACHAY1_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8593997.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3252..3743
                     /locus_tag="ACHAY1_002032"
     CDS             3252..3743
                     /locus_tag="ACHAY1_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8593998.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3767..6142)
                     /locus_tag="ACHAY1_002033"
     CDS             complement(3767..6142)
                     /locus_tag="ACHAY1_002033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8593999.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6468..7094
                     /locus_tag="ACHAY1_002034"
     CDS             6468..7094
                     /locus_tag="ACHAY1_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8594000.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7117..7821
                     /locus_tag="ACHAY1_002035"
     CDS             7117..7821
                     /locus_tag="ACHAY1_002035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8594001.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8013..8846
                     /locus_tag="ACHAY1_002036"
     CDS             8013..8846
                     /locus_tag="ACHAY1_002036"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8594002.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9043..10971
                     /locus_tag="ACHAY1_002037"
     CDS             9043..10971
                     /locus_tag="ACHAY1_002037"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8594003.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11109..11564
                     /locus_tag="ACHAY1_002038"
     CDS             11109..11564
                     /locus_tag="ACHAY1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8594004.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11644..11772
                     /locus_tag="ACHAY1_002039"
     CDS             11644..11772
                     /locus_tag="ACHAY1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594005.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12034..12303
                     /locus_tag="ACHAY1_002040"
     CDS             12034..12303
                     /locus_tag="ACHAY1_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594006.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12703..12957
                     /gene="rpsT"
                     /locus_tag="ACHAY1_002041"
     CDS             12703..12957
                     /gene="rpsT"
                     /locus_tag="ACHAY1_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8594007.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13046..14092)
                     /gene="holA"
                     /locus_tag="ACHAY1_002042"
     CDS             complement(13046..14092)
                     /gene="holA"
                     /locus_tag="ACHAY1_002042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8594008.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14130..16403)
                     /locus_tag="ACHAY1_002043"
     CDS             complement(14130..16403)
                     /locus_tag="ACHAY1_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8594009.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16408..16935)
                     /locus_tag="ACHAY1_002044"
     CDS             complement(16408..16935)
                     /locus_tag="ACHAY1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8594010.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELT"
     gene            complement(17071..17289)
                     /locus_tag="ACHAY1_002045"
     CDS             complement(17071..17289)
                     /locus_tag="ACHAY1_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8594011.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17286..17531)
                     /locus_tag="ACHAY1_002046"
     CDS             complement(17286..17531)
                     /locus_tag="ACHAY1_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8594012.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17647..>18367)
                     /locus_tag="ACHAY1_002047"
     CDS             complement(17647..>18367)
                     /locus_tag="ACHAY1_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8594013.1"
                     /translation="VMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFCIAS
                     GFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKE
                     PGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQ
                     LASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDL
                     QEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5632 a         3531 c         3220 g         5984 t
ORIGIN      
        1 ggaagcaaac ggttggtact aagattcatt atttgaacgt ttaaacacat tcaaaatata
       61 gtaagaggtc atgcgattta ttctttcgca tgacctcttt ttgagtttaa gtttagtctt
      121 tattccttaa ctatcgtttg ccagtatttc ttgtatcaga atctataaat ctactatttg
      181 aatcaatcta ttaggtttta aactgtccca ccttcacaca aatttttttt gatttatttg
      241 aatctggttc cgttgtttaa aatttttcct atattctttt agataatatg ttattttctt
      301 tctcatcgct ccaggtgtac cttctcttta taaaaaaaca aattatgcgg ctaagaagac
      361 tcgaacttcc acgaggtcac cctcactagc gcctgaagct agcgcgtctg ccaattccgc
      421 catagccgca tcacatcttt tagtttagga ttacatgtgt tttttgtcaa gaacatattt
      481 ttgttctttt tcatttctgt acgccctccc ttatgccatc tacacaaata attcatccga
      541 gcaactccat catctattga gggttggtga aaaaatctgt tttatccata tgatcgattt
      601 caatgttaaa cgaacgactc tgggattagt aactatttta tttttttctt catttctttt
      661 ttcctctttt tctcgattca gctatcttct ttcatttgga tattgattat ttcttctgtt
      721 gattactatc cttctggaat tctagacgct gaagtaattc acgttttctt tcttctagag
      781 atcataagta acgaatgaac gaacttcaaa taattttttt attgtaattc atatttgtat
      841 tgttcgatga ttcttaatac gatatgacac aataaataaa aataggcatg gctatttaca
      901 acaatagtgc tatagtagaa aacaaatgaa taaggttact taaagaggtg aatacgatga
      961 aactgatcaa actatgggaa gcagatttaa agaaagctta tcagctacaa actagctttg
     1021 gaccaaatga gagtggattt atcaacaatg cttatggcta tacatttgct gaatttttag
     1081 attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag agttacgttc
     1141 ccgcaaccgt ctttatttta gaaagtgatg aacatgagta tgtaggaatt tttaatttgc
     1201 gccataaact gaacgaaaca ttaagaaacg gcgctggcca tattggttac ggcatctccc
     1261 ctaattatcg taaaaaagga tatgccacac aaggattagc attagtacta caagaggcaa
     1321 aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa aaaaaatctt ggcagtctta
     1381 aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga tgagaaagaa tactatacga
     1441 aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa gggatttgaa tgtcctttat
     1501 caatagaaca catttattta attaacttat aatatagcag acaaaatttt ataaacaaag
     1561 aaacctttta tctcttttct tctagaaagg ataaaaggct tttttgtttt tcctatttgc
     1621 ttctcatagc tagtcagcca agatttctat cgtatcccca acgcaatccg tgcataacgg
     1681 ctcatcttat ctgtcgtcca tgctggatac cagactagtt tgacttctgc tttcgttact
     1741 tctggcactt cttttaatgc gtcatggata gattctgtta aaatatctgc taatggacag
     1801 cccatagttg ttagagtcat cttaatgact gtttctccat tttcaggatt gaattctata
     1861 tcataaatca agcctaaatt gacgatgtcg attcctaatt caggatcaat caccatttcc
     1921 aaggcagcaa gaatacgttc tttgattagt tctatttctt gtccagaccg ttcttggttt
     1981 gcttcgctca tcgtacgaca ctccttaaca cgatttttat ttatcatact ctttttttag
     2041 ctgcttgtaa atgagaattt agaagtaaga ttctcgttta aatttataaa aatgttctta
     2101 ttagatgtaa tagattaaat ttattctaaa ataaacctga ttcctcattt tttttgtgaa
     2161 cgatttttta tttctggcaa tacgccagtt gttcgataca ttttttgttc gttttcattt
     2221 gcaaaccggc caaatactga atgagttggt aaaatattga taaatgcatc gtcgtcgatt
     2281 tcatgaacga tctgatcaag atcatacatt tcgtagcggg tgatgaccat catgatcatg
     2341 ttcccttgtt tttttgaata gccaccgaca gacggcagta atgtcattcc acgtaccatc
     2401 cgttttgcta gttcttctgt tacctcttct ggcttgacgg taacgatcat tgctgttact
     2461 ttttgatgac tcgtgtggat ggcatcaatt acttgagtca ttgcataaat ggaaataatc
     2521 gtatacaagg cactttccca gttaaagaag aagcctgcga tgactacaat gataccattt
     2581 agaatcagca tgtagtttcc gactgttttt ccagttgttt gtgaaagtac aagagagatg
     2641 atatccatcc caccagttgt aaagcccatt tttaaagata atcctacgcc aagacctaat
     2701 aaaacaccgc ctactagggc attcatcaaa atattatcag tgatctgtcc gactggaagc
     2761 agcatcgtca tcaccgatac acatacaaca ttcacaaaac taaggatcgt tgctcgtttt
     2821 cctactttga gaaaccctaa tacaaacaca gggatattca aaagaaaaat aaatgttcct
     2881 gtgtctatct tgatatgaaa ccattcatat aaaagcgagg aaatgatttg agcgatccca
     2941 ttcatccctg cagaaaagac attggctgga atcaaaaaat aatttaaagc gaccgcagcg
     3001 gtcaatccgg ttatcaaaat aaccactatt ttctttaatc tttcattttt atgatacgca
     3061 gtcataattt tactcacaag cacctctccc ctactttggt attccaaatg taagaataac
     3121 agtttatcta ttaaaataaa atacttcgaa ctcaaatgag aagaatttta gcaaacaggt
     3181 aagaatctgc cctaatccta tgataagata gatgtatggt aaatatttat atgtatatca
     3241 ggagggttcc tatggcaaaa gatgctagct tcgacattgt atcagaagtc aatacagaag
     3301 aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc caatcgcttt gacttcaagg
     3361 attctacagt agaaatcaaa ttagagaaca gcaatctagt tgtattgagc gatgatgatt
     3421 tcaaaatcga acagatcaaa gatgtattat ttggtaagct gatcaaaaga aatgtgccaa
     3481 tcaaaaatat ccatttttcc gaatcagaac acgctctagg tggcaaagct cgacaaactg
     3541 ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa aatcactaca gctatcaaaa
     3601 atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca gattagagtg acaggtaaga
     3661 accgtgatga tctacaagct gtgattgctt tattacgtaa acttgactta ccaatcgagt
     3721 tgcaattcac taactatcgt taatcaaaaa acgaggctga cgtctgtcat tcctcgtttt
     3781 tttgatttac tgcttctttt gataaccaaa aacgctgtat tccttcttcc aattccccaa
     3841 cacttcgtac ttctcttgct gtttgccaat gttgtgggaa taaacgtcga taagaaaaac
     3901 tagaaaatcg ttgatctgcc aatacaacga ttccttgatc cgccatgcca cgaatcactc
     3961 gaccaccagc ttgcaagact ttgttcattc caggaagctg ataagcgtaa gcaaatccaa
     4021 ggtgttcctt ctcatcataa tatgatttga tcagttcctg ctcatgattc atctgcggca
     4081 aacctacacc tacgatcata ctgccgatca atcggtcccc tctcaaatcg attccttctg
     4141 aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc agcgggattt gtttgaaatt
     4201 ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt ttcttggatt agcacattgc
     4261 tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc taaatattga taagaaggaa
     4321 agaaaacgaa ataattcccg acctttccat cagcaaaagc atgaatcgtc tctactaatt
     4381 ttgccagact gtgttccctt tttcgataag tcgtctctag gtaatttgcg atgaaaactt
     4441 gctggtttcc ttctgggaaa ggactcggca gtttgtaagc cagacttttt tgtcccccca
     4501 gtgtttcctg ataataagag agtggtgaaa aactagcaga aaaaagaata ctgctttttc
     4561 ctttatccaa cgactgctca agaaacaagc ttggatcaat acaaaactct tttactatca
     4621 gatcgcgata cgttttttcg actgttgttt cgtagtgatc atcataaaat tcactgacct
     4681 tcaaaaaatg aagcaaatca aaataataag gaagcaattg ttcttgtgat gggtgctcag
     4741 gaaattctgc cagccattct ttaatttttt cgcttaattg ataacctgcc tttactaaag
     4801 attcagccgg tgctttttga tgatgataat cccaatgatc ttcttttgct atttctcgga
     4861 tcgaatcaaa ttcttttaat agtttgttga aacggcgatg aagttttttg aattcctttg
     4921 gtatagcttc cttacttcgt ttcgtttttt cataagatag ttctgccgaa tacatctctc
     4981 gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa cagatagtcc tcgttttttt
     5041 catcttcaaa aaaacgtctt aaataaacag ttggatcaaa taaataattg taatccccaa
     5101 tgattacgtc acaccagata ctgacatcta atgatagttc gaaaggacaa agtgtatgct
     5161 ttcttgcata agtttcgatg acttcacgag tgatctgatt ctcatgattc aaaagatccc
     5221 ataaaccttc attaatccga ttgtaataac cattggcata tgggcactga tctggattac
     5281 aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt caaagtgaca ctttttactt
     5341 cagctcctgt atcttttaat gctgtcatag catcttcagc tacttgtcta gttattgttt
     5401 tagccgttag ataaaagatc ttttctccct cttcttcccc tacagctttc aaagcgggga
     5461 ataacgtaga gattgttttt cctgttccgg taggtgcttc tacaaacaat ttctgtttcg
     5521 tacggatcgt tttataagca gctaccgcta gttcacgctg acctttccta tactcatcat
     5581 gggggaattt caaagccatc aaagaggcat tgcgtacagt gcgccagttt tcttgaaaaa
     5641 caagccattc tttataatct tcagtcagct ttttaaaaaa agtttctaac tgctctcttg
     5701 tctgatgttc aacctttctc gtgatcactt cttctgccgt ctggaagtaa gtcaattgca
     5761 gatttatttc ttgtaaatca tgttcttgac tatagatata ggcatacacc cttgcttgat
     5821 gaaagaaaag ttccacttgt tctggttcta aatcttcaaa tcgtggttcg gaggttttga
     5881 tttcatctat ataataaata ccgtctcttt tgaaaatacc gtctgcacgg ccttcaacga
     5941 tcaggtaata ttcatctaat tgaactgttg tttttaaaaa gacttctttt tcatagtttt
     6001 ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc ttctaatgca gtatggttac
     6061 tttttttacg attgtcgata cttccacgtc gcaaaataaa ttctactaag tgccgcacag
     6121 atatttttga ctgtttgatc atttccacca cctctttctc tctaagatta cctgaaaaaa
     6181 cctagtttag caaaaagagc tctctttcca aacataaaaa aacaccctgc tgattaggca
     6241 gggatatagg atatagaatt tggatttttg gatgaatcta atgtaacaag gacatattaa
     6301 aatgctatga aaacttactt tccttctctt tagaatatcc aaatactttt catatagtct
     6361 gataattcag ataagtaaag agaaataagt acctgttttt gtttcctagc aatgaatgcg
     6421 ttatactaat ggatgaaaga agttttggct atcaaggagg aatgcacatg catatcgaaa
     6481 gattaaaaac aggaatgatt gaagaaaact gttatttggt ctacaacgaa gaagcacttt
     6541 tgatcattga ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc
     6601 aacagccggt agcgattttg cttacccata ctcactacga tcatatcggt gcagtggaac
     6661 ctttacgcgc gtattatcaa atcccagttt atgtcagccc actagagcaa aaatggctag
     6721 gtgatccgat tttgaatctt tctggactcg gtagacatga tgatattgct gatgttattg
     6781 tctcgcctgc agaatatgaa tttgaaatga aaacttatac tttaggaaac atgtcttttc
     6841 gcgtcgtccc taccccaggg cattctatag gaagcgtcag ctttattttt gatgattttg
     6901 tcgtttctgg cgacgccctg tttagaggaa gtatcggtag aaccgatctg tatacaggga
     6961 atttagaaca attacttcat agtattcgga cccagctgtt tgtattacct gatgaatttg
     7021 cagtctatcc aggacatggc gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt
     7081 ttttcaatcg ttaaaacgac ttaggaggag aataaaatga cagaaactac actttatatc
     7141 gtacgacacg gaaaaacgat gttcaataca attgaacgag tacaaggctg gtgtgataca
     7201 cccctcacaa agcaaggaca agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc
     7261 gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt
     7321 ctaggagaac attctaaggg aaaagaaatt ccttatttta ttgaaaaaag gattagagaa
     7381 tggtgtttcg ggtctcttga aggtggatat gacatggaaa tgtggggtgt gatccctcgt
     7441 gtattgaatt ttaaaacata tgatgaaatg tttaccacag aagtttcttt tgaacaaatt
     7501 gctaatgctg tatacaactt agataccgct gggtgggcgg aaacttatga aacattgcga
     7561 gaccgtgtat ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga
     7621 aatgttttag tcgtatctca tggtttgacg atcgcctttt tgcttaatct gatcgatcct
     7681 aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac
     7741 ggtcgatttt ctatccacgg catcaatgat acgtcttata tcgataacgg gaaaacacat
     7801 agtcgcgact cgtttctata atggggatat tttaattttt accagagaat cataaaaggc
     7861 aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt gtacttatct gctttatttc
     7921 tcaagttttt cttaatatta aaaaacagat tgggtttctc aaaaaataac ggtatactat
     7981 aagatgtatc ttaattggga aggaaggaat ttatgttttt tgcaaatatt atcaaagcag
     8041 tgattctcgg tattattgaa ggaataacgg aatggctgcc catcagtagt actggtcatt
     8101 tgatcctagc agatgaattc atcaaactgg atgccagttc acaatttatg tcgatgttca
     8161 atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa
     8221 acccattttc tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca ctttggttta
     8281 aagtgatcgt tgcttgcata ccagccatca tcattggttt gcctttggat gattggttag
     8341 aagcaaattt ccacaagttc ttaccagttg ccattgtttt gatcgtttac ggtattgcct
     8401 ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct ctgaatgact
     8461 ttacgtacca agcagctctt cttgttgggt tattccaagt cttggcactt gttcccggaa
     8521 catcccgttc tggcgcaacg atcctaggat ctatcttgat tggtgcttcg cgttttgtcg
     8581 ctacagagtt ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga
     8641 tcgtcaaata tttagctgaa gggaattcat tcaaaatgga agaaactgtt gttcttcttg
     8701 taggaacatt cgtatctttt gtggtttccg tcttagctat caaattctta ctaagctatt
     8761 tgaagaaaaa cgactttacc gcttttggct ggtatcgtat tatccttggt atcattttga
     8821 ttggttattg gtttattgca atgtagaaaa aaccagaaca cttctcttat ttgagaaatg
     8881 ttctggtttt ttattttctt taataataat cattataaat aataaaaaca acatcaagct
     8941 taatcaccaa atgataacca ttatcgttct catttagctg ttattattat caattacatt
     9001 tttattatgc tatccttgtt ttgacaagga ggtctaataa cgatgactaa tcagaaaaaa
     9061 gcttttgcag caacaatcgg ctgcctttta tttatgatct tcggttttat cttcgaccga
     9121 ttctctctta ccatcacccc acttttttat atcagtgcca ttatcatcgg tggatataaa
     9181 caaacatcgg aaggcttgaa agaattgtgg aatgatcgga cactgaatgt ggatttattg
     9241 atggctttag ctgctattgg tgcctgtttg atcggtaatt actttgaagg agcaatgttg
     9301 acattcatct tttgtttaag cggtgcatta gaggaataca caactaataa aagtcaaaaa
     9361 gaaattactg ctttgatgaa tcttcaacca caaaaagccc aattattaaa agaaaatgga
     9421 caaatgcaag aagtagatgt ttctcaacta aaggtggata acttggtatt tgtcgctaaa
     9481 ggagcagctg ttccgataga tggcttttta gaaagcacac aggcaacgat cgatgagtct
     9541 gcattaagcg gtgaatctgt gcctgtagaa aaatatttag gcgatgagat gtatgcagga
     9601 acgctgaatc aaggaaatcc catcacgatt cgagtaacaa aaacgagttc cgaaactgtc
     9661 tttgctaaga ttatccaatt agtagaagaa gcgcagaata cgccaactca aacagcttca
     9721 tttatcgaac gaatcgaaaa taattatgta aaattgattt tgcttgctgt tcctctaatg
     9781 atccttctcc cccacttttt cctaggttgg agttgggatg aaagctttta tcgaggaatg
     9841 gtattattag ttgtggcttc tccttgtgcg cttgtcgcat ctgctacacc agcttctcta
     9901 gctgctcttt caaattctgc aaaaaatggg attttaatca aaggcggtat ccatttagaa
     9961 aagctttctg aattaaaagc gattgccttt gataaaacag gaacgctcac taaaggaaaa
    10021 ccagttgtaa ctgacagctt tttctttgat gaaaaggatt tggcacaaca acttcttgtt
    10081 gctatggaac aaaaaacgac gcatccactt gctcatgcga ttttgcaata tttcgattgc
    10141 acaatacctg aagaaatcca atacttacca atagaagaaa ttactggttt tggattacaa
    10201 actacctatt taggtgcgca atggaaagtt ggcaagcacg catatgatcc tgagacgatg
    10261 ataatcagca aagagattgc ggaaatgatc gaacgtttgg aaaatcaagg caaaaccgtg
    10321 atttatcttt ctaaagatca gcagttaatt gctgttttag gtctgctaga tattccaaaa
    10381 gcaaataccc aacaagtaat ttcctacttt aaatcccaaa acatccatac aagtatgata
    10441 accggcgacc attctggcac agcaaaggcc attgctgaac aagtagggat tgaacattac
    10501 tatgcaagct gtactccgga agaaaaaaca caactagtca aaaaagagaa tgaactttac
    10561 caagtaaatg cgatggttgg tgatggagtc aatgatgcgc ctgctttagc agccgcttct
    10621 ttaggtgtcg ctatggggca agggacagat gccgctatgg atatcgcaga catcgtctta
    10681 atgaaaagcg acttaaataa atttgtctac agtcatcggc tatctttaaa aatgcggcgg
    10741 atcatcaaac aaaatattat tttttcgatt cttgttattc tcttgttgat tgcttctaac
    10801 ttcctgcaat ttttaaatct acctcttgga gttgtaggac acgaaggaag cacaatattg
    10861 gttattctga atggcttgcg tttactaaga cctttacctg atctttctga ttcttcaaaa
    10921 aaatgtaagt cttgtccact atacaaagcg cggctgtctt ctgtcaaata agcaaagtgt
    10981 ttttttatga ttttgtttat tttatgaaaa aaacagctta tgaataggtc ttgaaggatg
    11041 aattcctact tttgtaactt tctaaaaaca tgttatgatt gtttcgaaga aagtgaggaa
    11101 tatatttcat ggaaagttgg ctttttttag gaattatttt acttgtagca attttagcta
    11161 aaaatcaaag tctgatgata gcggcagctt ttgttttgat tatgaagctc ataccagcct
    11221 cccgttcctt tttcccttgg cttcaaggca aaggaatcaa tattggggtg acgatcatca
    11281 cagcggctat tctaatccct atcgcaacag gagaaatcgg tctacgtgat ctattagaag
    11341 cctttaaatc ccctattggc tgggtagctg ttttttgcgg tgggctagtg gctgtcttat
    11401 cttccaaggg tgtaggattg attgcttctg atccacaaat cactgttgca cttgttttcg
    11461 gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc cggaccggtc attgcttctg
    11521 gtatcactta ctgtatctta caagtatttg ctttaggaaa atagaacaaa ttttgtctat
    11581 tttaggagat tcttaacacc cccaatttac tgatccgtga tatactgaca acgaggtgat
    11641 acaatgttcg aaaaattaaa gtatttcata ggaacaatcg gctggtttct tctagcggta
    11701 cttttgattc tgggcttagc ttatctatta tttcctgatt tgatacaaaa tgtgtttgat
    11761 actgcttttt gattgaccta ttaaaacgat ataggggatg ctcctgcgat gcaggggctt
    11821 tttttaatca atgttttgta ataatttagg ccgtgccaat gagacttcct atagaattcg
    11881 atgctctttt ttctttttgg ctcttctgct cttttttgtt atactagatt acagtggaca
    11941 aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa tggaagaatt agtgcctgtt
    12001 ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa cttttgaagg tcttttaatt
    12061 gatacgtacg ttttacaaga acagccaatg atgctgactg ggatcaactt gcagcttgag
    12121 gaccgtgtga tcaatggcct gcttaaagat cgctatttgg caaaatatat cttacgtttg
    12181 cccctaaata aatatgtgct ggaagtaagt ggcaaatgga accgtcggaa ccagttggtc
    12241 ataaaaaaaa tgttgataaa aaatatcgat gaatacatta aaataatcgg tacaccagaa
    12301 tagcctttac tgatcaaagg atactggtta taaaataact gcagtttctt aatagaaaaa
    12361 caaagaacaa cagggaatcg tatccctttt caaaaaaaac tttgcaaacg gttctctttg
    12421 ttatataata acttagtacc aaacattccc attcaaactt tgtttaaata gataaataaa
    12481 aatctagttt ggtttgacgc tattgcctcc cacaatagcg tctttttgat ttttttcagc
    12541 ttgtactatc cgtttttact gaaaaaaaat cctaataaaa gactgttaag gaaaaaaact
    12601 tgtcatgttc atattgcaaa gcacgctgcc tttaggtata ataatgaatg ttgaattatg
    12661 cggtaacacg cgtaagaact taagttatcg gaggtgaaac taatgccaaa cattgaatct
    12721 gcaatcaaac gtgttcgtac aagcgaaaac gctaatgtga aaaattcatc tcaaacaagc
    12781 gctatgcgta ctgcaatcaa aaaatttgaa gatgctgtag cttcaggtgc tgacaacgta
    12841 gatgcgttat ataaagaagc tgttaaagct attgatatgg ctgaatcaaa aggtctaatc
    12901 cacaaaaaca aagctaaccg cgacaaatct cgtttaagca aaaaaatcgc taaataaaag
    12961 aatcggcttc ggccgatttt tttatttgtc aaaaaaagga caaaaacaaa cgaccttccc
    13021 gcatgctttg ccctcgtctt tcttcttatc gatttcttaa caactgccct gtgcgcaaaa
    13081 cgaatagttg aaaacttaat tctttttcga tttgtcccgt tttgatttga taatccatct
    13141 caactaattg atcaaacaac tgtaccagca aatgttcatc gaattttttt acttcctgca
    13201 tggcaagttt tacacgatat ggatggattt tcaatgtttc agcaatattt gcctgctgat
    13261 aaccaatctt tacaagaaat tttgtctgta aaaataaacg taattgagaa agcaaaatcg
    13321 cattaatttt gattgtttct tccccttgca taactaaatc ttcgtataaa cgcaaagctt
    13381 gttccgtctt cccttttaaa atatattggg tcatgtcaaa aatattatgt tctaacgttt
    13441 taggaactaa ttcttctact tctcttaccg tgatttgctg ttgattttgt ccataaagga
    13501 ccaatttctg taactctctc atcattttgg ataaatctaa gtcggtcaac ctcaagaata
    13561 aatcaattgc acttcgttcc attttgattg aggaattctc taaggtattc atcagatatt
    13621 gacgtacttc tttttcattc aactgttgga cattgatgat aattgctgtt tttttcaatt
    13681 gtttcgttac ttttttccgt tcatccaact tttcatatgg cgcaaagaat actaatacag
    13741 tcgattctaa tggttctttc aaataagccg tcaataaatc agtattttgt tcgattccat
    13801 tgtttacttt ttcacctgtt aaaaaataag gattctctac gaatactaac cgttgatctc
    13861 cgaaaaacgg caacgtctct gcttcagcta ctacctcatc taaacttgca ttttccatgt
    13921 caaaacttaa aaaatttaag tcatcttcgc cctctaattg gatttttttg acaatttctg
    13981 tccgaacttg atcttggaga aacttttctg ttcctaaaat caagtaacaa gatgcaagtt
    14041 tttctttacg tattttttgt aactctgctt gtatactcaa attactcacc actttcaaaa
    14101 attatcctac catgagcaga tgagaattac tagtctatca ttgtttccac tagcccatga
    14161 ggatgaaaaa ctggatgcca gcgataggtg atcattcctt gttgatccgt tcgccatgtt
    14221 tttatctgat tttcttctag tgtctccaat acttcgctgt gcgggtgacc ataacgatta
    14281 tttacgccgc aggatataat cccatgtttt gcttcaatat gggaaagaaa atctgtgtta
    14341 cttgacgtac ggctaccgtg atgacctaat ttgactacat cggctttcag attaggataa
    14401 atcaaaagca attgtttttc cattgcttga ttcaaatctc ctaaaaataa aaagcgccta
    14461 ttgttatatt ccatccatag accaatcgaa tcttcatttc ttccctctcc cttttcaaaa
    14521 ggagtcaata cataaagatc ataataaccg ctgatcctgt ccccatgttt gatttcaatg
    14581 atctcagtac catttttttg agcttcagat aaatgccttt gtaatggctc atttcgtcca
    14641 gcgccccaac cgatataaat ttttttgata gtaaaatgct gagataacgt gggtaattct
    14701 cccacatgat ccgtatcgtc gtgtgtcaag atgagtttat cgattacact tatcccctga
    14761 ccttttagat atggaacgat atttctttca gcaggaggac gtatcatacc ttgcgcccac
    14821 ttttctcttt caaagtgtaa ctgacctcca gtatcgatca aaatcgtttc ttttcgaaaa
    14881 ggactttgca aaaagatact gtctccttga ccgacatcga taaaagcgat cgttgaagaa
    14941 aagcgtaaaa atggcaaagt caaaaaaata aaagaaaaaa gaccaacata aaacaagtgt
    15001 ttcacaggtt gttctttcaa cctatccata caaatcagta atccgaaaat atacatccca
    15061 gatagcacaa ggggcaaacg tcctataatg agagacgacc ctgaagaaaa atcgattatt
    15121 cgctcaaata agattaatcc ctcttctatc agcaaaagta aagtttccgg cagccagcga
    15181 ttgaagagtg tgagaaataa cacaccagga agaaaaagaa acttgaatac cggtgcaatc
    15241 agtaatgtaa aaagactacc taaaataggc cactcataaa aatgccatac aatcaacggc
    15301 atcgtgatca tagtaaacag gacgtgagag gccgtttttt tcaaccaatc tccatcaaaa
    15361 gaaatcagga taaacaaaaa actcatgaaa aaactaagct gtccacccgc atgaaaaaga
    15421 aatcgaggat caaatagtaa aagaagccaa aagacaactg caaataagtc catggtggaa
    15481 taatgcttat tcagcaattt gactagcaat cgcaataaaa acagcaaact agcacgcaaa
    15541 acagaaatag aacttcctgc aatagaaaca taacagatag ttagtagact caacggaata
    15601 atactgacgt gtagtggcca tccgatacgt cgaaacaaat aatacagcca ccctaaataa
    15661 aattggatgt gtagacccga tagactgaac agatgcagca aaccactctc cctaaaaatt
    15721 tcttctggtt ctctgaatcc tgcatcttta tagccccata ataaagaatt aatatataca
    15781 cttgtttttt gcggaaattc agattggaca aaggagataa aagatcctct ttttctctga
    15841 agccagtttc gccagtgatt ttgcatggtg atctgttcaa tcttttgaat ttggaaccgt
    15901 ccagaaattc tatggttcct gaagtaagct tcttggtcaa aattataaag attccgcttt
    15961 tttgctggag agatgaactc gccttcagca gtcaattcta ttccctttcc tcggaagtta
    16021 agccatgttt tcaattctgc ttctgtgttt acttgataag aaagctggag cttttgttta
    16081 ttctctgata accttccttc cattgtcaaa aaattaccat ttgcacgaat cgtatctgta
    16141 aatacagtaa tttgcgcaga tgttgttttt tcctctaaaa atgaatactc ttgtagccta
    16201 taagaaatgg ataccgttat gctacacaaa atacacaagt acgcgataaa acgtccttct
    16261 tttagcgaaa accataaaaa aattccggca agaagcaaac gataacccca attgtcagtg
    16321 taatacaaca aacagataat catcgacgta attgccggaa aaatcaaccg atttcgaaca
    16381 tgcctttcta aaacgctaga catgttttca tgttaattca ttttgaagtt ttgaaaaatg
    16441 ctgtgagtct aaagcaactt ggtgtacaga tacacctaat tgttcgatca acgcttgggc
    16501 ataaggatca tttcgatagt catggagata attgatccct ttcacaccag cttgcaacaa
    16561 agcctttgta caggccaaac atggaaaatg tgtgacatag atttctgaac cttccgtcgg
    16621 gataccaagt tttgcacact gtaggagtgc attcatctct gcatggatcg ttcgcaagca
    16681 gtgtccatcc acgacgtaac acccttcatc tatgcagtgg acatctccac tgactgctcc
    16741 attatagccg ccagcaatga tccttttttc tcgaacgagt gtcgctccta cttctaaacg
    16801 tgtacacgta ctacgtaaag ataacagtac tgcctgagcc ataaagtact ggtcccaagg
    16861 aatgcgttca ttatgtgact tatcagtaac tttttcttta ttcgtatcgc atgcttttga
    16921 agatttttct aacatgtttt atattcctcc gttttttcaa gttgattaaa aagtactcat
    16981 tgcttaaaca aaatattctt taaatataac gaatcatcct gtctgacaaa agatcctacc
    17041 cttcactgcc gaaatgaagt ccgtcatcca ttagcccatt ataaaaattc ttcgaatcca
    17101 tttgttcaag aaattccgta taaaaatcga tacttcacaa tcatggcgtt ttgctacttc
    17161 tgtgagaaca gagatatatt ctttgatcaa cttgttattc cgatattttt gttttgtctc
    17221 atcgactgct ggtggcgtga taaagaaagc atgatcagaa ctatagtctt ttttcagctg
    17281 aactatcatc cactgcagat tttttggtac gtttctttag aaagttgttt atgctctgct
    17341 agatcattcg tcccaatgag aataaatact ttatctgctg tatagctaag tacatctttg
    17401 gcaaagcgtt ctcgtagagc ttgactgtta ttgccgctga cagccgtatt cataatatga
    17461 atcccagata ttttattttg gagatagaca ttcagcattg gtttttttaa tgtttctttg
    17521 cgaacaatca agctatttcc tatccaaaaa tactacctcc tcgtcctgcc aagctcttag
    17581 aattggatta ttcttagtat ccattagcgt ccatttattg ctcgtgccag tcgattttga
    17641 atcattctat ttaattgtaa cgaagttttt tagtttttca actgtctttt caccgatacc
    17701 agaaatctcc tgcaggtctt ccactttctg gaaggatccg ttttcttctc taaatcgaat
    17761 gatttcttgc gctttgactg atccgatccc aggaatctgc tgaagttctg atggagtggc
    17821 tgtattgata tttattttgg gaggctcgga atctgctgta ggctgttttc cttccgagga
    17881 tgccaactga tcaagatgtt ctggcagctc ttcacctaca gcagggatat ataggacttg
    17941 ctgatcttct atcttcgcag aaaagttgat tcgatcgcta tccgcttctt ctaataatcc
    18001 acctgcacgc tttagtacat catagacccg ttcttctgaa gaaaaggaat agataccagg
    18061 ctccttcaca cttcctttga tatcggtgta catgaccttt ggacgagcat cctcttctac
    18121 atcgtttcct tcagctgatg gatcttctcc actggaacta aacgaagaaa gtatttcttt
    18181 tgcagcagtc tcttctgttt ctctataaaa tagcaaattt gataataaaa aaccacttgc
    18241 gatacaaaag ataagaacta tcatagacat ccaaatgatc aatttgggtg aagctagtat
    18301 tttcttgctg attttttcta tcattgttct tttttcaaag aatgaccagt aaaaatcatt
    18361 cattact
//
LOCUS       DAYYCM010000056        18020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000056 DAYYCM010000000
VERSION     DAYYCM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.057"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHAY1_002048"
     CDS             214..498
                     /locus_tag="ACHAY1_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8594014.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACHAY1_002049"
     CDS             complement(815..1198)
                     /locus_tag="ACHAY1_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8594015.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACHAY1_002050"
     CDS             complement(1200..1784)
                     /locus_tag="ACHAY1_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8594016.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHAY1_002051"
     CDS             complement(1831..3306)
                     /locus_tag="ACHAY1_002051"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8594017.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHAY1_002052"
     CDS             3487..3798
                     /locus_tag="ACHAY1_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594018.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHAY1_002053"
     CDS             complement(3913..6249)
                     /locus_tag="ACHAY1_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8594019.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHAY1_002054"
     CDS             6391..7059
                     /locus_tag="ACHAY1_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8594020.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHAY1_002055"
     CDS             7062..8105
                     /locus_tag="ACHAY1_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8594021.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHAY1_002056"
     CDS             complement(8342..9223)
                     /locus_tag="ACHAY1_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8594022.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHAY1_002057"
     CDS             9382..10110
                     /locus_tag="ACHAY1_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8594023.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHAY1_002058"
     CDS             10266..10583
                     /locus_tag="ACHAY1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8594024.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHAY1_002059"
     CDS             10576..11184
                     /locus_tag="ACHAY1_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8594025.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHAY1_002060"
     CDS             11239..12030
                     /locus_tag="ACHAY1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8594026.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACHAY1_002061"
     CDS             12168..12656
                     /locus_tag="ACHAY1_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8594027.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACHAY1_002062"
     CDS             12707..13579
                     /locus_tag="ACHAY1_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8594028.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACHAY1_002063"
     CDS             13755..14117
                     /locus_tag="ACHAY1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8594029.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACHAY1_002064"
     CDS             14126..14878
                     /locus_tag="ACHAY1_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594030.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACHAY1_002065"
     CDS             14892..16694
                     /locus_tag="ACHAY1_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594031.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACHAY1_002066"
     CDS             16971..18011
                     /locus_tag="ACHAY1_002066"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8594032.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5655 a         3126 c         3723 g         5516 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta
//
LOCUS       DAYYCM010000057        17715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000057 DAYYCM010000000
VERSION     DAYYCM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.058"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACHAY1_002067"
     CDS             4..816
                     /locus_tag="ACHAY1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8594033.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACHAY1_002068"
     CDS             813..1613
                     /locus_tag="ACHAY1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8594034.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACHAY1_002069"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACHAY1_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8594035.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACHAY1_002070"
     CDS             3110..4819
                     /locus_tag="ACHAY1_002070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8594036.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ACHAY1_002071"
     CDS             5031..9383
                     /locus_tag="ACHAY1_002071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8594037.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ACHAY1_002072"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ACHAY1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8594038.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ACHAY1_002073"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ACHAY1_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8594039.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ACHAY1_002074"
     CDS             11221..11514
                     /locus_tag="ACHAY1_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8594040.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ACHAY1_002075"
     CDS             11511..11822
                     /locus_tag="ACHAY1_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8594041.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ACHAY1_002076"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ACHAY1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8594042.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ACHAY1_002077"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ACHAY1_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8594043.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="ACHAY1_002078"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="ACHAY1_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="ACHAY1_002079"
     CDS             15292..17655
                     /locus_tag="ACHAY1_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8594044.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6037 a         3147 c         3902 g         4629 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DAYYCM010000058        16886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000058 DAYYCM010000000
VERSION     DAYYCM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.059"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACHAY1_002080"
     CDS             complement(<1..161)
                     /locus_tag="ACHAY1_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8594045.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINR"
     gene            complement(163..360)
                     /locus_tag="ACHAY1_002081"
     CDS             complement(163..360)
                     /locus_tag="ACHAY1_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8594046.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(754..2481)
                     /locus_tag="ACHAY1_002082"
     CDS             complement(754..2481)
                     /locus_tag="ACHAY1_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8594047.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2474..3667)
                     /locus_tag="ACHAY1_002083"
     CDS             complement(2474..3667)
                     /locus_tag="ACHAY1_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8594048.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3854..4498)
                     /locus_tag="ACHAY1_002084"
     CDS             complement(3854..4498)
                     /locus_tag="ACHAY1_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8594049.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4592..4726)
                     /locus_tag="ACHAY1_002085"
     CDS             complement(4592..4726)
                     /locus_tag="ACHAY1_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8594050.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="ACHAY1_002086"
     CDS             complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="ACHAY1_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8594051.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6857..7330)
                     /locus_tag="ACHAY1_002087"
     CDS             complement(6857..7330)
                     /locus_tag="ACHAY1_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8594052.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7731..7955
                     /gene="nrdH"
                     /locus_tag="ACHAY1_002088"
     CDS             7731..7955
                     /gene="nrdH"
                     /locus_tag="ACHAY1_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8594053.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8114..10273
                     /gene="nrdE"
                     /locus_tag="ACHAY1_002089"
     CDS             8114..10273
                     /gene="nrdE"
                     /locus_tag="ACHAY1_002089"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8594054.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10323..11288
                     /gene="nrdF"
                     /locus_tag="ACHAY1_002090"
     CDS             10323..11288
                     /gene="nrdF"
                     /locus_tag="ACHAY1_002090"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8594055.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11377..12516)
                     /locus_tag="ACHAY1_002091"
     CDS             complement(11377..12516)
                     /locus_tag="ACHAY1_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8594056.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12825..13538)
                     /locus_tag="ACHAY1_002092"
     CDS             complement(12825..13538)
                     /locus_tag="ACHAY1_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8594057.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="ACHAY1_002093"
     CDS             complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="ACHAY1_002093"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8594058.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14727..16259
                     /locus_tag="ACHAY1_002094"
     CDS             14727..16259
                     /locus_tag="ACHAY1_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8594059.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16450..16629)
                     /locus_tag="ACHAY1_002095"
                     /pseudo
     CDS             complement(16450..16629)
                     /locus_tag="ACHAY1_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16649..>16886)
                     /locus_tag="ACHAY1_002096"
     CDS             complement(16649..>16886)
                     /locus_tag="ACHAY1_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594060.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5234 a         3474 c         2917 g         5261 t
ORIGIN      
        1 aatctattta ttaaaggact gggatctaat tcatattttt gtttttctaa ttttaaatat
       61 accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca
      121 ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat
      181 tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca
      241 atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt ttccactatg
      301 agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat
      361 aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca
      421 aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt
      481 taagtatata aactatgtat attctttaat attttttgag aatgcacttc gtacataata
      541 aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa
      601 ttcataatag tttgtcggcg tctttgaacg tttcgaagat gagctacgca ttctcttttt
      661 ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat
      721 tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc
      781 ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc
      841 caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc
      901 ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc
      961 cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg
     1021 tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt
     1081 cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat
     1141 cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc
     1201 tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc
     1261 aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata
     1321 ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga
     1381 aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg
     1441 tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga
     1501 taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa
     1561 gatcactact actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt
     1621 tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat
     1681 gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg
     1741 caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac
     1801 cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa
     1861 aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc
     1921 tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag
     1981 agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc
     2041 tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat
     2101 actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga
     2161 gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg
     2221 gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga
     2281 aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag
     2341 taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc
     2401 agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg
     2461 atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca
     2521 cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg
     2581 gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca
     2641 cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat
     2701 ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt
     2761 cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca
     2821 atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa
     2881 attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca
     2941 tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat
     3001 catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt
     3061 gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct
     3121 caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact
     3181 gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt
     3241 tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca
     3301 agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac
     3361 ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac
     3421 tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc
     3481 cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca
     3541 caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca
     3601 tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt
     3661 ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca
     3721 cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac
     3781 tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta
     3841 atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct
     3901 gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg
     3961 ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg
     4021 cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag
     4081 ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct
     4141 gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga
     4201 tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg
     4261 ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat
     4321 ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa
     4381 gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg
     4441 ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt
     4501 tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa
     4561 aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg
     4621 ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg
     4681 cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct
     4741 tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa
     4801 acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc
     4861 aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag
     4921 cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc
     4981 gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta
     5041 tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca
     5101 aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg
     5161 actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg
     5221 aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag
     5281 ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac
     5341 ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc
     5401 aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt
     5461 ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga
     5521 tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg
     5581 aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc
     5641 atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa
     5701 agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag
     5761 tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact
     5821 gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc
     5881 cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg
     5941 atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc
     6001 acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca
     6061 ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata
     6121 aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt
     6181 aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt
     6241 accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct
     6301 cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga
     6361 acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt
     6421 ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt
     6481 tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc
     6541 ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta
     6601 gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc
     6661 acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg
     6721 ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct
     6781 acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt
     6841 ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg
     6901 actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg
     6961 ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct
     7021 tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat
     7081 caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta
     7141 tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata
     7201 aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg
     7261 ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta
     7321 aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata
     7381 tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc
     7441 tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata
     7501 gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt
     7561 tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta
     7621 ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt
     7681 ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa
     7741 aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa
     7801 atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga
     7861 aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat
     7921 tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga
     7981 tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt
     8041 tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag
     8101 gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa
     8161 tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt
     8221 tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttccatcta ttatggaaaa
     8281 aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc
     8341 atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt
     8401 tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta
     8461 tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga
     8521 agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc
     8581 ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca
     8641 agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg
     8701 tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa
     8761 aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt
     8821 cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt
     8881 ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg
     8941 cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa
     9001 aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt
     9061 ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa
     9121 acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg
     9181 ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat
     9241 catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg
     9301 caaacaggaa tatgaagtat taggaacaga tatctcatgt aacttaggct cgacaaatat
     9361 cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt
     9421 gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa
     9481 gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca
     9541 aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa
     9601 ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa
     9661 ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt
     9721 ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga
     9781 agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt
     9841 ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc
     9901 tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc
     9961 gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg
    10021 taaagtaatc gatgtttatg caacggcaca acaacacgtc gatcaaggca tgagtttgac
    10081 tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca
    10141 aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat
    10201 ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag
    10261 ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa aggagcattc
    10321 tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat atcattgata
    10381 aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa
    10441 atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat
    10501 ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta
    10561 aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg
    10621 ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag
    10681 aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg
    10741 aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat
    10801 tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta
    10861 acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg
    10921 gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag
    10981 actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat
    11041 tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca
    11101 aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc
    11161 caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg
    11221 gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat
    11281 tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac
    11341 agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac
    11401 ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg
    11461 gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa
    11521 tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt
    11581 cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa
    11641 aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc
    11701 tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac
    11761 cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc
    11821 aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc
    11881 ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc
    11941 aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa
    12001 aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa
    12061 tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt
    12121 gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc
    12181 atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt
    12241 catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac
    12301 tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa
    12361 accagcaaca agcaatggta aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat
    12421 attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca
    12481 gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat
    12541 acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa
    12601 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
    12661 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
    12721 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
    12781 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
    12841 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
    12901 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
    12961 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
    13021 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
    13081 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
    13141 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
    13201 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
    13261 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
    13321 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
    13381 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
    13441 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
    13501 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
    13561 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
    13621 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
    13681 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
    13741 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
    13801 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
    13861 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
    13921 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
    13981 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
    14041 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
    14101 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
    14161 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
    14221 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
    14281 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
    14341 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
    14401 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
    14461 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
    14521 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
    14581 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
    14641 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
    14701 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
    14761 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
    14821 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
    14881 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
    14941 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
    15001 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
    15061 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
    15121 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
    15181 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
    15241 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
    15301 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
    15361 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
    15421 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
    15481 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
    15541 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
    15601 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
    15661 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
    15721 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
    15781 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
    15841 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
    15901 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
    15961 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
    16021 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
    16081 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
    16141 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
    16201 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
    16261 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
    16321 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    16381 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
    16441 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
    16501 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
    16561 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
    16621 aaatacagac ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcctt
    16681 caaaacgtcg ttgtgctcga cttaatagaa cactgtaagc cagtaatatg ctttcaaatc
    16741 tactctctag ccctttaaca gaacgtgagt tgaaattttg acaacctaat ttttctaagg
    16801 aagaaaaaac agtctcaact gtttttcttt gttttccaag taagctgtgg tctagttttt
    16861 ctgatttatc catgttttta cgtggc
//
LOCUS       DAYYCM010000059        16771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000059 DAYYCM010000000
VERSION     DAYYCM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.060"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..1519)
                     /locus_tag="ACHAY1_002097"
     CDS             complement(308..1519)
                     /locus_tag="ACHAY1_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594061.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1638..2273)
                     /locus_tag="ACHAY1_002098"
     CDS             complement(1638..2273)
                     /locus_tag="ACHAY1_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8594062.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2409..3062)
                     /locus_tag="ACHAY1_002099"
     CDS             complement(2409..3062)
                     /locus_tag="ACHAY1_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594063.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3165..3371)
                     /locus_tag="ACHAY1_002100"
     CDS             complement(3165..3371)
                     /locus_tag="ACHAY1_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8594064.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3447..4193)
                     /locus_tag="ACHAY1_002101"
     CDS             complement(3447..4193)
                     /locus_tag="ACHAY1_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8594065.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4353..4577
                     /locus_tag="ACHAY1_002102"
     CDS             4353..4577
                     /locus_tag="ACHAY1_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8594066.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4601..5362
                     /locus_tag="ACHAY1_002103"
     CDS             4601..5362
                     /locus_tag="ACHAY1_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8594067.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5374..5631
                     /locus_tag="ACHAY1_002104"
     CDS             5374..5631
                     /locus_tag="ACHAY1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594068.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5723..5974
                     /locus_tag="ACHAY1_002105"
     CDS             5723..5974
                     /locus_tag="ACHAY1_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8594069.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5950..6123
                     /locus_tag="ACHAY1_002106"
     CDS             5950..6123
                     /locus_tag="ACHAY1_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594070.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6237..6644)
                     /locus_tag="ACHAY1_002107"
     CDS             complement(6237..6644)
                     /locus_tag="ACHAY1_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594071.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6805..7029
                     /locus_tag="ACHAY1_002108"
     CDS             6805..7029
                     /locus_tag="ACHAY1_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594072.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            7039..7233
                     /locus_tag="ACHAY1_002109"
     CDS             7039..7233
                     /locus_tag="ACHAY1_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594073.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7236..8258
                     /locus_tag="ACHAY1_002110"
     CDS             7236..8258
                     /locus_tag="ACHAY1_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8594074.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8221..9036
                     /locus_tag="ACHAY1_002111"
     CDS             8221..9036
                     /locus_tag="ACHAY1_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8594075.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            9054..9836
                     /locus_tag="ACHAY1_002112"
     CDS             9054..9836
                     /locus_tag="ACHAY1_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594076.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9836..10684
                     /locus_tag="ACHAY1_002113"
     CDS             9836..10684
                     /locus_tag="ACHAY1_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8594077.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10681..10923
                     /locus_tag="ACHAY1_002114"
     CDS             10681..10923
                     /locus_tag="ACHAY1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594078.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11066..11434
                     /locus_tag="ACHAY1_002115"
     CDS             11066..11434
                     /locus_tag="ACHAY1_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8594079.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11449..11610
                     /locus_tag="ACHAY1_002116"
     CDS             11449..11610
                     /locus_tag="ACHAY1_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8594080.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11607..11933
                     /locus_tag="ACHAY1_002117"
     CDS             11607..11933
                     /locus_tag="ACHAY1_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594081.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11930..12745
                     /locus_tag="ACHAY1_002118"
     CDS             11930..12745
                     /locus_tag="ACHAY1_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8594082.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12884..13195
                     /locus_tag="ACHAY1_002119"
     CDS             12884..13195
                     /locus_tag="ACHAY1_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8594083.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13192..13437
                     /locus_tag="ACHAY1_002120"
     CDS             13192..13437
                     /locus_tag="ACHAY1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594084.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13434..13850
                     /locus_tag="ACHAY1_002121"
     CDS             13434..13850
                     /locus_tag="ACHAY1_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8594085.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13847..14185
                     /locus_tag="ACHAY1_002122"
     CDS             13847..14185
                     /locus_tag="ACHAY1_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594086.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14182..14412
                     /locus_tag="ACHAY1_002123"
     CDS             14182..14412
                     /locus_tag="ACHAY1_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594087.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14419..14622
                     /locus_tag="ACHAY1_002124"
     CDS             14419..14622
                     /locus_tag="ACHAY1_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8594088.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14619..14915
                     /locus_tag="ACHAY1_002125"
     CDS             14619..14915
                     /locus_tag="ACHAY1_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594089.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14992..15405
                     /locus_tag="ACHAY1_002126"
     CDS             14992..15405
                     /locus_tag="ACHAY1_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8594090.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15418..15804
                     /locus_tag="ACHAY1_002127"
     CDS             15418..15804
                     /locus_tag="ACHAY1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594091.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16355..16582
                     /locus_tag="ACHAY1_002128"
     CDS             16355..16582
                     /locus_tag="ACHAY1_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594092.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16579..>16771
                     /locus_tag="ACHAY1_002129"
     CDS             16579..>16771
                     /locus_tag="ACHAY1_002129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594093.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5683 a         2734 c         3495 g         4859 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaaataa
       61 aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg
      121 gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagttcgt
      181 tatttaatgg tattttaaat gaagataagc gaatttacag catttttaaa tgcttatata
      241 ccgcaaatta tcccaagttt ttgtatatcc tacccgagtt tatacccgaa aatataattt
      301 tttagtgtta ttttatatct ttttttaata ggttttcaaa cgctgtagtt gctgtatctt
      361 tttctttgcg taagacatgg ccataaacat tcattgtagt attaaaatct cggtgtccca
      421 atcgtttttg aagctctttt ggagtcatat taggatcgct aagcaaatag gtagctgatg
      481 tatgtctaat gtcgtgaaaa cgaatgtgac gcaaattgtg acgtttggtg aatcgtttga
      541 acttcttata cagataactt ccacgtggca aagttccatc ttgttttgta tcaaatataa
      601 aatagtgttt taatttcttt atatttaatt tccaacgaat ctttttattc tctttgatta
      661 attcatctaa catatcaaat agataagcag gaccggttac tattttttca tcatcatcat
      721 ttttcaaacc tggcagcaac cgaatatctg attttccatc cacttctgaa atccgctgat
      781 gaaatcttat ttcttgtttg tcaaagtcaa tatctttttc ttctaaagct gcaatttctc
      841 cttgtcttgc tcctgtaata aatgctagaa caattaaagt tttaatttct atgctttcct
      901 caaatgctgc ttttagcatt gcatgaattt cttcttccga ataaggttct tcgatattct
      961 tttttacgcc tttttctttt ggtattttta cattttccac aggattttct ttaattgctc
     1021 cgtactcatt tttagccatc aaaaataagt tgttaacggc gtataacata cgaagctttg
     1081 tatttcttga taaaggttta tcagagtttc tcccaggatc ttttgtattg attctttgag
     1141 cattaacaac aattttcttt atcatgtatg gtttgatatc gattagaggg ataaaatcaa
     1201 atttatcaag aaatctattc tcgatcattc gcttatagtt atgataagac tttggctcta
     1261 aatttttttc ggcctctgaa agccatattt ttctataaaa ttcaccaaaa gttatattga
     1321 acaaatccaa cgaatcatcg gtcattcctt cgaattgctc taaccagatg ttcaattctt
     1381 tatatgccgc tcttttacta cttgctttta tagttttgct ttttcttttt gccgagccat
     1441 caggtttgta tcctaataca gctcttagtc tatatgtttc gttgtctact tgttctaagt
     1501 atcctgttac ttctgacata attgaatcag ctctctttct ctgatacaat aggtactata
     1561 aagaagccta tcgtataggt ttgtttcttt cttagaacac gctcgctttg gtcggtgggg
     1621 cgtgtttttt atactaatta gttgtgaaga gcaattgaaa ctgtctctgt tccaaagtaa
     1681 cctgtttgtg cttgcaatac attatttttt gcatttgctt gggcttcaga gatatcaaaa
     1741 actattttac cagtttgttg catatcagga tttaagcttt ccataaagaa agagttagtg
     1801 atattaccat tttcatcttg atttgccgac atagatgcag ccgaatcagc ttcgaaagtt
     1861 tttccatctg cttttaattt gaagaatgag ctatcaacag tcacagcctt atcacctgca
     1921 ttttttacag ataagtcaac tactaagaaa gtgtcttttg cgttagtagg aagtacagat
     1981 gggccaactt gttttactac ttcaacggag tttactttgt attccatttt ccctacagca
     2041 acgtcatcac cgattttata agtcttttct tcttttgttg tagtttcttt tgtagtttct
     2101 ttattagtac ttgttgatgt tgcagtagct gaatctttgt tttcaccgcc acttagagca
     2161 ccaccaatac caaaaaacaa aagtactatt aatacccaaa accatacccg tttataaaac
     2221 ggttttttaa ccttatacat tttcccatct tgacccataa ctttttttgc catttaaata
     2281 ttcctcattt cttgttataa tatatttgtg atctcagaaa tgaggtatga gtccgtgttg
     2341 cagcacgggc ttttttattt ataacttttt tagagattat aggcaaaata gtagggcata
     2401 aaaatatatt attgaattcc gtatttaaaa aatcctaatt gaacttcacc ggaagtcttt
     2461 tgctgtgttg tacgcaatgc ttcttcttca gacattccat tctgtacttt ccatgcaaca
     2521 ggcgacatcc cgtatttgtt aacaaaatca gtaagtgata aagtgtcagc gtcttgctga
     2581 gcgcttgttt gttggtcttc tggattttgt tgagatgctg cttgttgttc tttctgatct
     2641 tgactgataa tattgccagc atcatctgta gtcaatccat tttcataaag ggccacgccg
     2701 aaagcttccc actctttgtt ggaccaattt gcacgatcag ctggagttga ctgtaaagtg
     2761 cgttgtttca tctgttcata tgtttcttct tgaggtgcgg tttggattgt actctgactg
     2821 gagcttataa ctgttgggct aggttccgcc gtaacttggt tggagcttgt aactgttgaa
     2881 ctagaatctg ttttagatgt agatttgcta gtagatgaac tggtttcagt tgtttctttt
     2941 gttttactta tttttgtttc ttggttagaa gtggcatctg ttgattcagc ttttttatta
     3001 tttgaacaag ctgaaagtag cagagcagta cttaacaaca acataacgct aacttttttc
     3061 attttatact cctcttttct ttgatacaat aagttgtaaa gaagcctact ataggtttaa
     3121 catcccacgt ccacaaactt tggtcggtgg gcgtgttttt ttgtttatag actttcgaaa
     3181 actaaagtag cttggattct atcgccacca ccgaaacctt tactaccacc gtttgtagtg
     3241 gaaatagtgt gtaatcgata acctttagaa cactgtctat ttatagtgtt ttcaagttct
     3301 gataggtttt gcgacccttt tcctataaac ttttctttta atacaacttg caatacaacg
     3361 taacttggca tattcttttc cttctttcta ctatgataat aaatactaac ccctagccgg
     3421 agcgccaact acgactgggg tattttttag aatacgactt tcccgattat tctaatctgt
     3481 tccggtgaga cgactctatc ttcatataat tcgtttatag agcgtagaat aatcgtttga
     3541 ctctcataat ccttatatac ttctttacac gtcacaccgt caccatctat ttcaacgata
     3601 gcaatctcac cgtcttccac gtctggctgt tccttaacaa atacaaattg tccattcctg
     3661 ataactggtt ccattgaatc gcctataata ggaacagcaa aatcagcatt gtttggaact
     3721 ctttcaaatt cttcttcctc tacaactgta tcgccataaa ctaattcagt cgggttggcg
     3781 gcagaagctg aatttttgat tgtcggaagt ttttcctttt taggaaagtg caccacgttt
     3841 cctcgatttt gctcttcgag ttgctgttca gcaaagttgt agacttttgt ttgtcttggt
     3901 ttttctaatt gattgtagat tgtttcaatg gatggaatgt tatttttctt atcttcttta
     3961 ggaacatcgt aaccaagcaa ccatgcctca ctcacattta aagttttagc caacaaataa
     4021 agcttgtgtt ggtcgggcga tgacttacca ttgacatatt gcgatagatg actttttgac
     4081 atttttattc cagtttcttt ttgcaaaggt aaagacatat tcaaaatatc aacttgcttt
     4141 aaatttcttt cagacattaa ctgattaagt cgttgctgag tcgtttgctt catcttatac
     4201 ctcctttctg atacttatta tataccttat tgaacataag ttcaataaaa aagttaaatt
     4261 aatttgaact tttatgttga caatgtttta tgcaggagtt atactttggt tacaaagttc
     4321 aaaacatttg aactagaaag gagcgaacta atatgagttt tgattattct aagttgtcgg
     4381 gaagaattgt tgagaaatat ggaacgcaat acaatttttc aatcgcttta ggtttgtcgg
     4441 aaagaagttt gtcattaaag ttgaataacc gagtaccatg gaaaagcacc gaacttcaaa
     4501 aagccattga attgctagat attccaaaag aagaaattgg cgaatatttt tttaatttag
     4561 aagttcaaaa tgtctgaact taagaaagga attcgaaaaa atgaacacac aaaaatttga
     4621 aaacaatcta tttgaattag aagttaagac ggaaaatggt gaatcgttat ttaatgctga
     4681 aacagtagca agaagtttgg gtttcgtgga aacaaaaaac ggtaaagaat atgttcggtg
     4741 gagaactgtc aatggatatt taaaaaaata cctttcgcaa gaagttgcga aaaacgactt
     4801 tatcagtgag ccaatggttt acaaattagc ctttaaagca aacaacgtac ttgctgaaaa
     4861 attccaagac tggttagcaa cggaagtcct acctcaaatc cgaaaacacg gcatgtacgc
     4921 aacagatgaa ctacttaaca atccagactt gctgattgaa gtagcaacca aactcaaaga
     4981 agaacgcacg ttacgattga tagctgaaca acgagtaaat gaattacaac cgaaggcaga
     5041 ctattacgac cgaatcttga acaacaaagg attagttaca gttagtacga ttgctaagaa
     5101 ctacggtatg agcgctgtat cgtttaataa attgctacat gaattaggta tccagttcaa
     5161 tcaaagtggc acatggctgc tatacagtaa gttccaagac aaaggataca cgcatattga
     5221 gccatttgat tatgaagata aaaacggcaa tcggcaagta aaaacaagga tgaaatggac
     5281 gcaaaaagga cacatcttct tatatgaaac attgaagaag aataattact taccgatgat
     5341 tgaacgtgaa caaacagcat aggaggtaac actatgaacg aggaaatgag ccaaacaatc
     5401 aaggtacaaa aaatgataga tgatctaaca catgcaattg atagccaagc cgataaaatc
     5461 atcaaggaat tgcaaggaca aaaagtttgg gatgcaaaaa tgcttttcaa gactatcaaa
     5521 tttgaaatga atccaacaaa aagaaagctc gccgatgtgt tggaagaaaa gttggcttcc
     5581 gctatcaacg agcaagaatt actatttgaa accgatactt ttaacagctg atgttttata
     5641 gatgacagtt tcatccgaat ctatatcatt atagattcga ttattgcaat gtacgaatta
     5701 taaatacagg aggaaataac caatggagca actagcttta gttaacctct cagaccttaa
     5761 agcttctttg gcagaatctg agattgcaaa tgaagtgtgg gatacaaagc aagctgcgga
     5821 atatttaaaa accacaacac gcacgctgac taaagatgcc gaatctggaa agattccagc
     5881 agcgaaagtt ggcagagagt ggaggtttag tagtatcgct ttgtatcagt atctaaaagg
     5941 aggaaaaaca tgggtaaatt caacagagca ctagtgttca gcgcacccct aatcgtctat
     6001 gctttaggac tttggggaag cagacaagca ttaatcggca cgatcgttta catggtttgg
     6061 atttttatgg ggcttgatga agctgagtac agagcgaaaa agcccaccga gggggctgac
     6121 taaagtgtgg ttctgtttat taggtgttta tctcgtggcc gttttagtaa ataactacat
     6181 gagaaaacgt ggcgaatact ggtatacatc ctatgcggtt ctagtatgtc taatgcttac
     6241 aatttttcta atgatttatt caaaataggt aatactttgt caatcaaata agtttcaaga
     6301 gatttttgtt ctgcatcagt gttgccgtaa ccaaaacggc ttaagaaaat tttcataacc
     6361 tctatatctt cattttgaat atatggcagg actgcataac ctgatgcttt tacagcagat
     6421 atatttttat cagtgttact accaatacaa atacccacat tgttgagaaa tgttgagaaa
     6481 gttgtttgta ttgtttgggt ttttgaatcg tgtttttgta attcaatttc tttatccttc
     6541 atcgattcaa catgcttatt atttataacc gctgtaatcg aaggggatat aagagctact
     6601 aaagctagga taatcgaaat tgtgatcgtg ttatcgaaac tcattttttc accatccagt
     6661 ttttaaacca ttatatcaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa
     6721 aataaaaaaa gacacgaacg ccagcaagca tgatcgtgtc caaaagaaaa atcttttaaa
     6781 ccattataac acaggaggaa ataaatgatc tcagttaaag ggttaggcga tgaaatattc
     6841 gaagcaatga tgagcaaagc acaacaggac atcaaagaaa aaatcatttc tgctgcagcg
     6901 caaggacaaa ctagctgcac tgttcgatcg agaggaacga caccaagttt tcttttggcg
     6961 ctagaagatg aaggcatttt gaatatcaaa cgaagagatg gcagcgtcag attgttttgg
     7021 caattttagg aggccgtgat gaaagatttt gattctttag gcgccagaca gcaaccacca
     7081 aatgaagcta gtcctgttgg agttgattgg caagaaaatc cgttatatcc aggtgatact
     7141 tgctatctaa cagaagaagg ctatgtacca gtagacgcaa ttctagagta cgtccagcaa
     7201 cattatccaa agattgaatt aggaggaatt taggaatggc aaatgattta acacaaacaa
     7261 cacaacgttc attagatgag caggtaatca gtaatcttgg acggttacaa gaacaaggtt
     7321 tagaaatgcc accaggatat agtccacaaa atgcgttaaa aagtgctttt ttcgaactca
     7381 ctaacaatac aggaggaaac ttgttgcaga tggctgcaaa caatcaagaa atgaaaacct
     7441 ccatttctaa tgcactttta gacatggtta ttcaagggtt atctccagca aaaaaacaat
     7501 gctatttcat taaatacgga aacaaggttc agctaatgcg ttcatatttt ggaacaatgg
     7561 cggtccttga tcgtgtaact ggaggggcag atattacgcc tgtagtcgtt cgacaaggtg
     7621 atgagtttga agttgcaatg gatggaccaa acatggttgt caaaaagcat gaaacaaaat
     7681 tcgagaattt agacaatgaa atcattgcag cctatgtagt catcaagtta acaaatggta
     7741 aagagaccac aacagtcatg acgaaaaagc aaatcgatca aagttgggct aagtcaaaaa
     7801 tgaaagattc tggaccgcaa aaggaatttc cagaagaaat ggctaaacgg acagtaatca
     7861 atcgggcagc taaaacattg atcaacacaa gcaacgacaa cgatttatta gttcaagctg
     7921 caaaagatac gttagaaaat gaatttgaca atgatcggaa agatataacg ccacaaacag
     7981 aaaaagtggc tactctcgaa caaaaattct tttcaaacaa aaagattact gaaccaatac
     8041 aaaaagaacc agatccgatt gtgattccag atgatatcca aaatgaagta actcgagttg
     8101 ctgatgtacc tgcccatcca gaaatagagc aagcacattt gatcgaaaat gaggatatca
     8161 atccaattca agaagaattg atagatattc cagactttgg acgcgaggaa ggtgtagaag
     8221 atgtctcaga atttgaagac gacgagtacc ctttctgatg agaattatta ctccaatgaa
     8281 gctgactggc agtacatgtc aacatcacaa tataagtcct ttttaaaatg tgaagccgca
     8341 gccttagcaa agttgaaagg cgattgggca ccgacgtcgg atcccaaagc cttactcgta
     8401 ggaaactacg tacattctta ttttgaatca aaagaagtac atgaagcgtt caaagaagaa
     8461 aataaatcca aaatgttttc tagtaggaag ccgtatggat tgttgaaaga tttccaaatt
     8521 gctgaacaaa tgattgaacg acttaagcaa gaagaagcat tcatgaacat ttatcaaggt
     8581 gagaaagaaa cgatcgtgac tggggaacta tttggaacga catggaaagg caagattgat
     8641 tgcttgaatg ttgaggatga gtattttgtt gatatcaaaa caaccaaaga catgcatgag
     8701 cggaaatgga acgaaaacta cggatcaaga gaaacgttca ttgtcaattt tggttacgta
     8761 cttcaaatgg caatctatca agagcttctt ttacagcaat acggaaagat gttcatgcca
     8821 atcatcgctg cggtatcgaa gcaaacgcct agtgaggcga agttgataac gattgatcaa
     8881 gataagatga gctacgagct aatcatgcta aaagaaaata ttgaaagaat tgtcaaagtg
     8941 aaaaatggcg aagaaaggcc gataccttgc ggtacgtgcg agtattgtcg aggtaacctt
     9001 tcaattactg gattcactag catggacgat ctttaaaagg aggtgctttg cgaatgaatc
     9061 taggatacat caagttatac cgtaaagtga ctagttcatt cgtttggacc aattccgata
     9121 tgttcaagct atggatattg tgtttaatga aggctagtca tgaagataga aaatttttat
     9181 ttaacggtca agaagtacgt ttgacaagcg gacaattcgt cacaggagcc catgcaatag
     9241 caaaagagta caacgaagga gtcccgagtg acaaagcaat tgcatggcga acgctatgga
     9301 gatggcttaa gaaatttgaa aatgaagaat tattgacaat ccaatcgaac gctagatata
     9361 gcgttataac aataataaat tggtctgact atcaatcgag tgacaagcca gtgacaagcg
     9421 acggacaatc gagtgacaag ccagtgacaa caaacaagaa tgataagaat gataagaatg
     9481 ataagaataa taatcctcgc aactctcgaa aaacacgaga gtatgcagat gacgatccaa
     9541 ataaaaaatt ggccattctt ttattaaaac tcattcgaaa gaatcgtagt ctcaaagaac
     9601 ccgatttgaa caaatgggcc aatacgattc gtttgacgat tgaagctgac aaacgaactg
     9661 gaaaagaagt tcaagacatg attgtttggg ctactaacca tgatttttgg tccacagtca
     9721 tttcatcgcc aactagctta agaaaaaatt tcgatacaat ggtgggtcaa aaaaataaaa
     9781 gaaagcaaca aaatatttct aatgatgagt taccagaaac aggtgaggat tggtaatgaa
     9841 taaaaaacta agtgcaatgg ctgcacctta cggcggatta aaaacagcag atcataattg
     9901 cccgaaatgt ggtgatccat tgtatatctg gaaaacaaaa aataaagatg gtactgatcg
     9961 atgcggtcct acatgtatca ataagatttg tggttatcga gaaatggtaa ctaaaaatca
    10021 aaaagaagct atccaaaaag cgaatgaagc aatgaaaaag gacgctatca atcgaatgct
    10081 taacagttca atgattacag atgatgccat atggaccttc gattttgatg gatacaaagt
    10141 agttgatcag gaaacagcac aaataaaagc gatggctcaa gaatgggcta aaaaaattgt
    10201 aaatggtagc acgattcatg cagtgataac cggtagaaca ggagccggca aaacccatct
    10261 aggcgctgca gtgattaaag aagtcatgag ggcatctaat tataaaattg cttgttcatt
    10321 tatcagttat cgagaacttt tagaacagtt gaaatttgca atgaatgatc cagaagcaag
    10381 aaaagccgta acaggttcgc tgatggctga gataaagaaa tcagatttcg tggtaatcga
    10441 cgatttgggt gcagaactag gaagaatgga agaaaacaac caggcaacgc cttatgatgt
    10501 tgatgttctc acatcgctca cagaagctcg tctaaacaaa gctacgatat tcacaaccaa
    10561 tttatcatcg aagcaattaa aacacgcgta cggtgagcga gtgttctctc gtgtaatgaa
    10621 tggaacgaaa gggaacatag ctgtattcaa aacaacgaca gacaaaagga ggaatccagt
    10681 ttgacctttg tagtgaaaaa aatgtgctac ttagatagcc gagggcgagg agaagctagt
    10741 gtcgagtttg ctaaacacca cacgactaaa gaagaggctg accttgttgc aagtgtttgt
    10801 ggcggcgaag tagtcgaagt tattaaaccc aaaagacgat tttcaaaacc taagacaaag
    10861 ccagtgaaaa aagagtgttg ttgtccgaaa agtaaccaag catggatgag aggtgcaaaa
    10921 tgacttgttt aaaatgcaac gatgaaacag ttatttggta taagacattg cttggatggt
    10981 caacttgtga accttgtcca atatgcaatg aaaatggacg acgttcgaaa gaacgactcg
    11041 aaagactaaa gaaggagtat agcaaatggc aacaagaagc aaatacggaa acaaaaagca
    11101 cgaagtagac ggcatcactt ttgattctat cacggaagca gagtattacg atctagcctt
    11161 gtggcaagca caggcgaatg gttggaaagt gaagctccag gaaaggtttg agctgatgcc
    11221 gaagtttgaa ctagagggca agaagtatcg caagatcgag tatattcccg acttcacatt
    11281 ttacaaaagc ggcaagctgg tcaaagtcgt agatgttaag gggatgcaga caaaagactt
    11341 taagatcaag gcaaagttgt tctgtcatca atatcaagtg ccgttgatat tagccaaaaa
    11401 atatcggaat acgttcaagg aagagcgttt ttaacgaggt ggtccatcat gacaacagaa
    11461 gaagtgattc aaatgcgaat tcgaagcctt cggcgtgaga ttgacgatct ggaacgaaca
    11521 aaggcagtga tggtcaatga aacggctaga aaggcaatcg atttgcacat agagaattta
    11581 agaagggaaa tccgtagatt ggaggaatga gcgtggataa ggaagcggct tggcgaaaat
    11641 taatgttgct gattcaagat gagaactggc aagaagatga agcagtggtt gctgaagttc
    11701 agcgtctagg aaagattgct aacggacgta tacgaaaaaa gccagacaaa agaaaacagc
    11761 gcaaagggaa aatcgtcgtt gttttacacg aaggcaaaat tttgatgcaa ggaacagcta
    11821 gtgagctgtc tgcagaaact ggatatacgc gtgggactat tcgaacgtac gcttggcgaa
    11881 atcacaccga tcgaaaaggg cacgaatata agtatttgga ggaagaaaaa tgaacgaaaa
    11941 caaattaatc aaattgggtg tagcaggagc agtaatcgta ggtattggag ttatcggagg
    12001 atttaagttc ttcgaaaaaa tcgataatgg atatgtgggt gtgcgctatt caatgaacgg
    12061 cggtatcaaa gatgaagcac tgacgcaagg tgtgaaattt gtagggattg acaaagtgat
    12121 ccaatatcca attcgcttgc aaactatcca atcaaaaaat atttcagtat ctacaagcga
    12181 cggcaaaaag acaacgattg atatcaaata tgactacaaa gttgattcaa ctaaagcagc
    12241 aaaaatgtac aaggaatttg ggaatatcac ttcggaagat atcgaaagtg gatggttaaa
    12301 atctaagctt caaaaggtcg ctcgtgaagt ttatgcgaaa tatagtctgc ttgatgtcct
    12361 ttcaggagat tcctctaaag ttgaagctga ggtattaacg aactttgcta aatcagttga
    12421 atctaaaggg tttgaagtcg aagacgtaac acttggtgtt ccagatgtcg ataaagaaac
    12481 acaaaaatca atcgatgcga tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga
    12541 cgcagaaact gcaaaaactc aagctgatag tgaagcttac aagaaaacaa aagctgcaga
    12601 agcagaggca gaatctaatc gcaaagtcgc cgaatcagta acagacaatt tgattcgtta
    12661 tgaagaagct caagctcgca aaaagcatgg atgggtaaca gtaaatggag cagatactgt
    12721 agttacggat gaagcaggca aataatatgg gattctttat ggctaaaatt ctcttgttct
    12781 taggtttagt aggagcagca tatctcgtgt atgcgctcct ttcccaaact gatgacaaag
    12841 aagatgacaa caacgatgaa atgaaatttt aggaggagaa ataatggacg aactaatcac
    12901 aaaagtagag cagtgggcta aagacaaggg actggatcaa gcagatccaa aagcacagtt
    12961 tttgaaagta gctgaggaat tcggggaaat agcttcggcg atggcaagaa gtaatgatga
    13021 actatttaaa gatagcgtag gagacgttat cgtcacgctg attatccttt ccatgcaaaa
    13081 agggacaaac gtacaagagt gtttagaaat ggcatacaac gaaatcaaag gacgcacagg
    13141 gaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gagggcagca aatgataaaa
    13201 aagctcattc aattcagcat ggatttatat gatatcgaat caggagcaac actatctgtg
    13261 gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt gtgggtagtt
    13321 gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc ggttgagttt
    13381 gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag cgaatgatac
    13441 cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc gtcttgcatt
    13501 ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc gaattgaaat
    13561 catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat aagaatggtg
    13621 tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc gattacttag
    13681 ataataaagt ttgtattgtc aaaaattcaa tagattattc cggattagtt tgtgccactg
    13741 ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa tacacgtatg
    13801 aagtcatcgg aaatatatac gagaatagcg agttattgga ggtagagtga ttatcttagc
    13861 tactgactac atcgattctt taaaagatga aaatgggaat gtcccagcat tgctaaattg
    13921 tggaattaaa agcaagaata gaatcatttt gttacggata tcggctgatt tagcaaaata
    13981 tctttatctg agcaagacag cagtatatta cactgcggct acacgttatc agtacaaggg
    14041 aaaaacaatc agtcaggatt attatgatcg tttaatcagt cctgatatgt atgggaattc
    14101 agaatcagca attaagctgt ttggcgcaat agagatatat gccgatgatt ttccaaattt
    14161 atggttgaag gaggaagcgg aatgaaatac gaaataccac taagtgaagc agacgtccag
    14221 tcaattatta acggtcggga ggttaataaa aaacttcctg atggtactga attagtcatc
    14281 agacaaagtt atttgaaaga tatggcagct ccagtattaa ttgatcgttt taacgtgact
    14341 gattctgtgg tagagaacca cttgaaagaa tttcgatcaa gtataaacga cactttcaga
    14401 ttagggagtt gattgacaat gaacaccaga caccgcagag tagcaaaact aagaaaacag
    14461 gaactgaatg tactaaagac aaagtttgaa aaagaatatg gaatttcagc agaagaaaca
    14521 tataaagtgg caagtcagtg tgttgctgat gcaagtgatg ctattcgtaa gtttggaatt
    14581 tcgatattaa gtgatgatcg taaatgggag gaaaaagaat gaaactaaaa gacggatttt
    14641 acgctagcag tcacggtatc ggcggtttaa tgctagatat gccgacaaag aatcctaaaa
    14701 cacgtaagaa accaaaagtc aaagtcggtg acatggtccg ctgtgaagca gaggagttca
    14761 tttatccatt tcgtggatat gtagaacacg tctataatca ctcagcgatc attcgcattg
    14821 aaaacacgat ggaatgtgat aaatggacag ctaaaagcaa agagaattta gcagtagtga
    14881 gattggtgga tatggaacta atcaatgaca aataaaaaag ccggatcgct ccgactaaca
    14941 taataaaaca gacaagttta ttatatcaca taaaaggagc agtttgaatt gatgcaattg
    15001 ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt
    15061 cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg
    15121 gatatgccga aggcaccgaa gcacggcaat aaggcagagg atgcattgat tcagatgatg
    15181 gatatagaag cggagagaga cgcgatttta gcggctttga tggctcttag tctgattagt
    15241 cgtcagatac tctactacag tttttgtgtg ccagacagct tctcaaacta cagaattagc
    15301 cgtgaagtgg gttattcaga aagaagtata caacggatga aatcggaagc tctaatagag
    15361 tttgcagaag catataaaca cggaagaata attgcttata aataggggga ccatataatg
    15421 tggaaagatt atgtatcgtt gaaagagttg aaaaaagatc ttgttttcaa aagaattgta
    15481 gaatggtcag agagtgaatt aattttagaa gatggaacca aaatggaagt tgtatgtagc
    15541 gaatcagatt gctgcgcatg ggccgaaggt gaattcaaaa atgtaaagct tgatgcagtg
    15601 attacagata taaagatttt tgataaaggt aaccgtctct ataatggtga cggacatacc
    15661 tcttatgctg aagtcgttgt ttatcataat cgtaatgaga tcgctaaagc agaatgcaca
    15721 gcgaatgatg ggaatggcgg ctactattat agtgtttgtg ctttaaaggt caaagacaaa
    15781 ctttgtatag tgactgacgc ataaaaaaat ggcggttttt tggcggaatg atggcggttt
    15841 ttagccattt atcagtgata ttatggtagt gtcgaaagat tagtgatagg tctgagacaa
    15901 aataataata aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc
    15961 aacggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc
    16021 tatcatccgt tgctgtttat tgttacatac agatcgctta ggcggtcttt ttattttggc
    16081 cgaaaaccta cattttcgat ggccgattat tggaggagga atagcatgtt caaactatct
    16141 gaaatcatta agaagtccga cgttgagaaa ttagagatgt taaaaaagaa gttgaagaaa
    16201 tagcagtctc ttcgtgaggc tgttttattt tgctcacaaa aatagaccac tatcgggtaa
    16261 tagtggtcag gaattaaatg aaaaagatgt tagggttgtt agctaagtat aacatcataa
    16321 cgcttacaaa acaatgcaag aaggataaat tattatgaga acttactggt atgtgtcatt
    16381 aaacaataaa tatccgaagc caatgaaagg acagcatagg cgtgtagtca tgtctgttca
    16441 aatgaaggcg aagtattcga ttgtagaaat gctaagagaa gctacaccaa aagagattga
    16501 tcagtataag ttggttctta ttggcattgg ttggtttaag gatgaacata ttcaaacaaa
    16561 catgaagagg tggttgagat gaagttatat tatgttgaaa aagcaaattg ttgggtggtt
    16621 gaaggctgtc tgtcaaagta cgctacagat attatatcta ctcaattttc agagttggga
    16681 ttgaaggtta tcttttgtgg tgagtttgtc agtgtgaaag aagcggtatt gccaatgaca
    16741 gcaaagagca gacgctcgcg tggttcaagt t
//
LOCUS       DAYYCM010000060        16203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000060 DAYYCM010000000
VERSION     DAYYCM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.061"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1008)
                     /gene="murB"
                     /locus_tag="ACHAY1_002130"
     CDS             complement(73..1008)
                     /gene="murB"
                     /locus_tag="ACHAY1_002130"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8594094.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1081..2001)
                     /locus_tag="ACHAY1_002131"
     CDS             complement(1081..2001)
                     /locus_tag="ACHAY1_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8594095.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2114..2866
                     /locus_tag="ACHAY1_002132"
     CDS             2114..2866
                     /locus_tag="ACHAY1_002132"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8594096.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3730..4263
                     /locus_tag="ACHAY1_002133"
     CDS             3730..4263
                     /locus_tag="ACHAY1_002133"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8594097.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4753..5277)
                     /locus_tag="ACHAY1_002134"
     CDS             complement(4753..5277)
                     /locus_tag="ACHAY1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8594098.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5270..5743)
                     /gene="tsaE"
                     /locus_tag="ACHAY1_002135"
     CDS             complement(5270..5743)
                     /gene="tsaE"
                     /locus_tag="ACHAY1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8594099.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5866..6846)
                     /gene="pta"
                     /locus_tag="ACHAY1_002136"
     CDS             complement(5866..6846)
                     /gene="pta"
                     /locus_tag="ACHAY1_002136"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8594100.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6999..7679)
                     /locus_tag="ACHAY1_002137"
     CDS             complement(6999..7679)
                     /locus_tag="ACHAY1_002137"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8594101.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7805..8674
                     /locus_tag="ACHAY1_002138"
     CDS             7805..8674
                     /locus_tag="ACHAY1_002138"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8594102.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8700..9215
                     /locus_tag="ACHAY1_002139"
     CDS             8700..9215
                     /locus_tag="ACHAY1_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8594103.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9262..9825)
                     /locus_tag="ACHAY1_002140"
     CDS             complement(9262..9825)
                     /locus_tag="ACHAY1_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8594104.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9880..10059)
                     /locus_tag="ACHAY1_002141"
     CDS             complement(9880..10059)
                     /locus_tag="ACHAY1_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594105.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10136..10645)
                     /locus_tag="ACHAY1_002142"
     CDS             complement(10136..10645)
                     /locus_tag="ACHAY1_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594106.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10741..11121)
                     /locus_tag="ACHAY1_002143"
     CDS             complement(10741..11121)
                     /locus_tag="ACHAY1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8594107.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11451..12200
                     /gene="pnuC"
                     /locus_tag="ACHAY1_002144"
     CDS             11451..12200
                     /gene="pnuC"
                     /locus_tag="ACHAY1_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8594108.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12384..13661)
                     /locus_tag="ACHAY1_002145"
     CDS             complement(12384..13661)
                     /locus_tag="ACHAY1_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594109.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13740..14384)
                     /gene="pcp"
                     /locus_tag="ACHAY1_002146"
     CDS             complement(13740..14384)
                     /gene="pcp"
                     /locus_tag="ACHAY1_002146"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8594110.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14583..15527)
                     /locus_tag="ACHAY1_002147"
     CDS             complement(14583..15527)
                     /locus_tag="ACHAY1_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8594111.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15529..>16203)
                     /locus_tag="ACHAY1_002148"
     CDS             complement(15529..>16203)
                     /locus_tag="ACHAY1_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8594112.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
BASE COUNT         4660 a         3367 c         2872 g         5304 t
ORIGIN      
        1 cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac ttattttaag
       61 ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt gtttttctcc
      121 aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta cttcttggat
      181 gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc
      241 agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg
      301 gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc atgaagggta
      361 ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg ctttgattgc
      421 ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac gtaattcttg
      481 gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc
      541 atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc catacgcacc
      601 tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa attcaaatcc
      661 agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc
      721 aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga tgactacacc
      781 gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca tccaaggtat
      841 atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt tcgggaatgc
      901 aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa
      961 caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg aggattttcc
     1021 tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg ccagtcttgt
     1081 tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat gacttcttac
     1141 aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct gagaaaagaa
     1201 ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt cgccaaattc
     1261 tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt ttacttcata
     1321 agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg ctaaatccat
     1381 cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc
     1441 ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc
     1501 tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc
     1561 tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac
     1621 cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa
     1681 ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg
     1741 gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtggat
     1801 catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa
     1861 cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc
     1921 aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac
     1981 accgattaca ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac
     2041 accatttttt tctaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag
     2101 gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa
     2161 taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac
     2221 gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta
     2281 cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc
     2341 cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata
     2401 ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt
     2461 agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca
     2521 aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa
     2581 ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg
     2641 actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt
     2701 ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga
     2761 ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga
     2821 cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa
     2881 agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat
     2941 aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa
     3001 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg
     3061 tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta agaggttgtg
     3121 aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
     3181 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
     3241 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3301 ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa
     3361 gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca
     3421 tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
     3481 aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc
     3541 acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat
     3601 cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg
     3661 ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt
     3721 tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt
     3781 gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca
     3841 ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa
     3901 gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt
     3961 caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga
     4021 tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt
     4081 agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg
     4141 aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc
     4201 ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag
     4261 taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca
     4321 caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga
     4381 ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag
     4441 taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac
     4501 cgcagatgca agcttttgga caccgtttac caagaggttg tgaaatcagc gttttgacct
     4561 gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     4621 accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt
     4681 tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt
     4741 ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag
     4801 atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag
     4861 gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat
     4921 ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca ttccccaata
     4981 ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc
     5041 cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt
     5101 attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc
     5161 tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat
     5221 gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat
     5281 tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga
     5341 ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat
     5401 aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac
     5461 cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca
     5521 tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt
     5581 tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg
     5641 cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct
     5701 ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc
     5761 tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta
     5821 gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt
     5881 ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa
     5941 atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa
     6001 acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt
     6061 tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga
     6121 agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt
     6181 agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct
     6241 catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc
     6301 gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata
     6361 tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg
     6421 ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc
     6481 agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc
     6541 ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt
     6601 atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt
     6661 tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg
     6721 ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc
     6781 ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa
     6841 ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa
     6901 ataacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata
     6961 caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc
     7021 caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg
     7081 gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata
     7141 tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac
     7201 acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa
     7261 cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa
     7321 aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga
     7381 tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc
     7441 acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc
     7501 accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct
     7561 ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa
     7621 tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa
     7681 aactccacct cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt
     7741 caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat
     7801 cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag
     7861 tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat
     7921 ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga
     7981 ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt
     8041 taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg
     8101 tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat
     8161 tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat
     8221 tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc
     8281 tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc
     8341 agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc
     8401 ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc
     8461 ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt
     8521 caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat
     8581 cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa
     8641 agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga
     8701 tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa
     8761 acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat
     8821 tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat
     8881 tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat
     8941 tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata
     9001 tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag
     9061 caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag
     9121 tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg
     9181 ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat
     9241 acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact
     9301 gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg
     9361 atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg
     9421 taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat
     9481 agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc
     9541 atctagctga tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc
     9601 atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg
     9661 tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt
     9721 ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa
     9781 tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt
     9841 tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat
     9901 cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg
     9961 gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc
    10021 aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat
    10081 ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg
    10141 cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac
    10201 gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga
    10261 ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa
    10321 gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga
    10381 ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct
    10441 ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt
    10501 ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca
    10561 tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag
    10621 caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat
    10681 caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct
    10741 ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc
    10801 tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat
    10861 tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata
    10921 gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt
    10981 cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc
    11041 taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg
    11101 atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat
    11161 taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta
    11221 taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa
    11281 tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac
    11341 acaaattatt tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg
    11401 ttagtaaata cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat
    11461 attttcagtt tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct
    11521 ttttcagtct tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg
    11581 ttattacatt tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa
    11641 aatcagtcaa cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt
    11701 cagcaaagaa ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc
    11761 ccgtattgtt aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg
    11821 gaaaaagctg gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc
    11881 tgattggtcg ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta
    11941 gcttgactgc tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag
    12001 cttcagggct tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt
    12061 tggcgatgtt cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt
    12121 caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca
    12181 acgcacaaca acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc
    12241 tcacgctcta aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga
    12301 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
    12361 gcttctgtcc cagcctctat tttttatagt aattccttca gatactttct tgcctcatca
    12421 ttgaaacgca gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca
    12481 gtaaaacgat aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg
    12541 atatctttca ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca
    12601 gattgtatag cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga
    12661 ggaatgctgt cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat
    12721 agatagattt ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg
    12781 acttctggtg ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa
    12841 gcattaggat gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat
    12901 gcgcgatagt tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca
    12961 aatagttctt tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc
    13021 ttgatttcat tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga
    13081 aattctgcga gccgctgagc taattttata tccggtaaat atggctgttt catccgacga
    13141 ttttggtaca agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc
    13201 tgcttaagcc ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc
    13261 gaagctgcca ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt
    13321 aggatctttc cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac
    13381 tcacaataca atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca
    13441 gaaaacaccc aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga
    13501 tatcggcggt gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc
    13561 caacgttgac tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct
    13621 tttggaaacc attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct
    13681 aattacaaaa agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat
    13741 tagtgagttg ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact
    13801 gcataagtca aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga
    13861 tcgatcactt gatcgggtga gaatggcacg tggatgaacc cacctcgaat tgccgggaat
    13921 tgtgtttcga tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct
    13981 gtataagaga tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt
    14041 ggcaatgagg taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga
    14101 tttccttcat tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag
    14161 actgctgacc gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg
    14221 atggcttctt tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg
    14281 atttcagcac cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact
    14341 ttgtccccac caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc
    14401 cgattgattt aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa
    14461 aggaaacaat agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata
    14521 gtgttccact acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc
    14581 tattatttaa atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac
    14641 aactggtttt tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata
    14701 ttgaagttag cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc
    14761 attcctatta acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc
    14821 gtcatcacgg tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca
    14881 agagcgtaaa tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca
    14941 ttggaaatca ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc
    15001 attggcaaca tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat
    15061 gtacttggtc gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg
    15121 acgcccacgc cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca
    15181 ccgatactca aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata
    15241 tattcttttg atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg
    15301 gtcatcgcaa agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata
    15361 actaccccta agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct
    15421 gttaaatttc ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc
    15481 agtaatttat cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt
    15541 tcctggataa tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac
    15601 aactaaagcc gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc
    15661 atatcctaga tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag
    15721 tacttggcca aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc
    15781 ttcatgctta ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa
    15841 aatgattgga cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat
    15901 cattcgaaag atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt
    15961 gatcaaggaa gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat
    16021 aatgatgata aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa
    16081 cattccttga atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat
    16141 caatatcgag tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct
    16201 aag
//
LOCUS       DAYYCM010000061        15900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000061 DAYYCM010000000
VERSION     DAYYCM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.062"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHAY1_002149"
     CDS             complement(52..522)
                     /locus_tag="ACHAY1_002149"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8594113.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHAY1_002150"
     CDS             complement(681..1139)
                     /locus_tag="ACHAY1_002150"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8594114.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHAY1_002151"
     CDS             complement(1310..2455)
                     /locus_tag="ACHAY1_002151"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8594115.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHAY1_002152"
     CDS             complement(2468..3385)
                     /locus_tag="ACHAY1_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8594116.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHAY1_002153"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHAY1_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8594117.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACHAY1_002154"
     CDS             4477..4647
                     /locus_tag="ACHAY1_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594118.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACHAY1_002155"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACHAY1_002155"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8594119.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACHAY1_002156"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACHAY1_002156"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8594120.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACHAY1_002157"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACHAY1_002157"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8594121.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACHAY1_002158"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACHAY1_002158"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8594122.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACHAY1_002159"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACHAY1_002159"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8594123.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACHAY1_002160"
     CDS             9222..10223
                     /locus_tag="ACHAY1_002160"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8594124.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACHAY1_002161"
     CDS             10220..11581
                     /locus_tag="ACHAY1_002161"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8594125.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACHAY1_002162"
     CDS             11541..12692
                     /locus_tag="ACHAY1_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8594126.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACHAY1_002163"
     CDS             12667..13836
                     /locus_tag="ACHAY1_002163"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8594127.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACHAY1_002164"
     CDS             14011..15390
                     /locus_tag="ACHAY1_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8594128.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACHAY1_002165"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACHAY1_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5201 a         2803 c         3384 g         4512 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttataaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAYYCM010000062        15665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000062 DAYYCM010000000
VERSION     DAYYCM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.063"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1422)
                     /locus_tag="ACHAY1_002166"
     CDS             complement(<1..1422)
                     /locus_tag="ACHAY1_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8594129.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGN"
     gene            1674..2855
                     /locus_tag="ACHAY1_002167"
     CDS             1674..2855
                     /locus_tag="ACHAY1_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8594130.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2915..4543)
                     /gene="treC"
                     /locus_tag="ACHAY1_002168"
     CDS             complement(2915..4543)
                     /gene="treC"
                     /locus_tag="ACHAY1_002168"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8594131.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4994..7705)
                     /locus_tag="ACHAY1_002169"
     CDS             complement(4994..7705)
                     /locus_tag="ACHAY1_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8594132.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7715..8416)
                     /locus_tag="ACHAY1_002170"
     CDS             complement(7715..8416)
                     /locus_tag="ACHAY1_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594133.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8602..9201
                     /locus_tag="ACHAY1_002171"
     CDS             8602..9201
                     /locus_tag="ACHAY1_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8594134.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9282..10295
                     /locus_tag="ACHAY1_002172"
     CDS             9282..10295
                     /locus_tag="ACHAY1_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8594135.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10403..11320
                     /gene="rbsK"
                     /locus_tag="ACHAY1_002173"
     CDS             10403..11320
                     /gene="rbsK"
                     /locus_tag="ACHAY1_002173"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8594136.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11348..11743
                     /gene="rbsD"
                     /locus_tag="ACHAY1_002174"
     CDS             11348..11743
                     /gene="rbsD"
                     /locus_tag="ACHAY1_002174"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8594137.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11743..13116
                     /locus_tag="ACHAY1_002175"
     CDS             11743..13116
                     /locus_tag="ACHAY1_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8594138.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13473..15617
                     /locus_tag="ACHAY1_002176"
     CDS             13473..15617
                     /locus_tag="ACHAY1_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8594139.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
BASE COUNT         4614 a         3128 c         2912 g         5011 t
ORIGIN      
        1 atttcctgca tataaatgaa aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa
       61 ttgaatatat ctaaaagcgc cactttcctt atcttctgct tcgaatagat accttacgcc
      121 acggtttcct gcctcgtagg tcaagatttc tttccctaca taacgataat ttgaacgttt
      181 cgttgttcca ttcttaaacg taaatgccat ctctttctca ctgatcttga ttgaagcaat
      241 atctgtttga tagcccttcg tataatattc tttatattct tgtgctgtct tatcctgatt
      301 caatttttct ttatattcaa aaacaggatc aaaagtccca tcttctacat acgtatctac
      361 tgtttgccaa gttcctgccc aatctgaaag agagcgatct tgtacatcct cgtcttcaaa
      421 ataaccgttg tagacgctct tttctttttt gggggtcaaa gtatcttcta gcgggttttc
      481 tgtctctgtc gtttttttca acgcttcgag gtttgcttcc atgatagaaa gatagttttc
      541 tccattttct atttgttcat tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac
      601 acctgcttcg tttgctaacg ttctggcaat actatctttg gcgttttctt caaaataaat
      661 atagttgatt ccatgttctt taacaaagtg tttcagttca gcaattctcg ccgcagacgg
      721 ttcttcacta gaagaaagac cagcaatcga tacttgattc aatccatatt ctaacgctag
      781 atagttgaat gcagcatgtt gcgtgacaaa agatctttgt tttgcttgct taaaggtttc
      841 ttgataaagc tgatggagtg cttgaagttt ctcggtatag gctgctgcat tttttgtcca
      901 tacttcctgt ttttctgggt aagcttcaat cagctgatca cgtatgtttg ctacctgctt
      961 gattgctaaa cttggagcta gccatacatg tgggtctagc tcatgtgtat ggccttcttc
     1021 cccgtgttca tggtcatgat catgtccttc ttcacttccc ggaagtaaaa gcataccttc
     1081 tgtcgctttt atcgtatgga cattgccttc ttgtaatgtt ttttggattg cggggaccca
     1141 tgtttccatg ttttcatcat tataaacaaa gatatctgta tcttggatct ttgccatgtc
     1201 tttagcagaa ggttcataat cgtgggaatc agtccctgct ggaatcaata actctacttc
     1261 tccttcatcc ccgacgattg cttttgtaaa atcatacatg ggataaaagg tcgtcatcac
     1321 gctcaatttt tctttactgt cctcggcttt cccacttgcc ccgcaagcag ttaacatacc
     1381 tgctaaaacc agcatggcca atatacctat caatcgtttc atttatgaca ctcctcttct
     1441 catcttcttt catttgaatg ttccgattcg cttttcgcaa accgtaatag ttacgtttta
     1501 gcaacaggat ttaatttatc atatggttaa aagaactgtc aagcgttttc ttattttaat
     1561 tgatgagaaa gtttatatga atgattgacg atatttttgc aatcaacctc tatcattttc
     1621 cttttatacc cgcttttgtc tataatgaag ttatagataa aggaggaaaa agagtgaaaa
     1681 aagaaacaga tacaacgaat cgtttgatcc gctttttagg aggaaaaacc tcttactatg
     1741 ttttaggaat agttatctta cttgcgctga tcatttattt tttccatcag atctcgttta
     1801 tattcaagcc tgtcggaatc attatcgcta ctatcttgcc cccattattg tttgcactga
     1861 tcttgtatta tcttttggaa ccagtcatca aacgtttgag caagcacctt tcgaggaact
     1921 tgtcagtcat tatcgtttat gtgattattt tatttttatt ggggcttgga agcatatggc
     1981 ttattccttt tcttgaagaa caggcaaaga cattgatcag tagtttgcca gatttgtttc
     2041 aagattttca agagagtctc cgacaatttt tggagaaaac accttttgcc gattcattta
     2101 atcaattttt cgcttctatt gacgacgtga cgtctgatat agctggattt attggaaatt
     2161 attgggaaag tggcgcacaa cggatcagca acattttttc aactgttaca gctattttta
     2221 ttactttatt tactggtcca attgtcgctt ttttcctttt gagagatcct caaaaatttt
     2281 atcagtcagt tttagcgatt gtcccaccag catttcgtac agattttaaa aatttgacaa
     2341 agattgctaa tcaacaattg ggttcatttc taaaggggca aatcattgct tcatttatcc
     2401 ttggagcagt ctactgggtt tgtttcttac tgatcggatt agaatttgct agtgttttag
     2461 ctatttctgc tgggttgtta tgtatcattc catatattgg gccgtttatc gccttctttc
     2521 caggtttgat cattgcattc caagactcta cttttatggc tgtcaaattt gtcattgtct
     2581 ggtttgccgt acaacttctc cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt
     2641 tacagatcca tccgattacg atcttaatcg ttttacttgt catgggtgat ctaatgggaa
     2701 ttgtgggcgt tatttttggt attcctatct atacgttagt caaattactc gttactttta
     2761 ttttccgtaa attcaaacaa cgctacaata aattttatgg cgataaagga gagtatgaaa
     2821 actcttcatt ttctgaagaa gattatttta aataaaacac aaaacgtcga tcttacgaga
     2881 atagctcgta ggaacgacgt tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct
     2941 ggtatggttt tagctccaga ctttgatgaa gaacagtatc ggaataatta tcgataagaa
     3001 tattggcttc tgtgaatcct tctgggatgg cgatccttgt atctttatca tagaaatttg
     3061 ttaatacaag cagctgctgt ccttcaaatt ggcgaagaaa gccataaact tccggatgat
     3121 ttgcttcata agcatgataa tctccttgtg caacgattgg atatgttttt ctcagccgaa
     3181 tcaatttctt gtaataggac aaaatagaat cttcagaaga tagttctttt tctacattaa
     3241 tctcttctag cttgcctcca acttttaacc aaggtttccc tgtagtaaat cctgcttgtt
     3301 ctgtgtctga ccattgcatg ggtgttcgag agttatcgcg agattttgct ttgatccgac
     3361 gaaacgcttc tttttctgtg aatccttgtt caaggagtaa cttataagca ttcaaacttt
     3421 ctacgtcaat atagtcggaa atcgatgaat agtctgggtc gatcatgccg atttcttctc
     3481 ccatataaat atagggcgtc ccacggttca aatggatcat ggttgctagc atctctgctc
     3541 cttgttttcg atactgatcg atagctacaa atcgattgag cgcacgtggc tgatcatgat
     3601 tattaaaaaa tagagcattc cagccgttac cctcactcat cttttctccc catgtatgaa
     3661 atagtcgctt taattctgca aaatcaaatg gaggagtaga ccatttattc ccattttcat
     3721 aatcgacttt caaatgatgg aaattaaaga ccattgataa ttcttctcta ctaggttctg
     3781 tatacaagat acaattttcg atcgtcgtag agctcatttc accgactgtg atgatagtgg
     3841 aatccgtccc aaacgttgct tggttcaatt cttttagata cgtatgggtg attggtttgt
     3901 ccgtatattc aggtttgcca tcattttctt tattattttt caattgttcg tctttaccga
     3961 taacattgat aacatcgaaa cggaatcctt ttaccccttt ttccatccaa aaacgaacaa
     4021 ctttgaatag ttcctctctt actgcttggt tccgccagtt caagtcagct tgtgtccgat
     4081 catataagtg caaataatat ttccccgtat ctccaaatgg cgaccaagca cttccaccaa
     4141 attttgatac ccaatccgtt ggctcttctc ttaaaatgaa gaagtcctga taatgctggt
     4201 cccctgctag tgcttttttg aaccattcat gttctgtcga aacgtgattc agcaccatat
     4261 cgagcatgat ttctatccgg tatttctttg cttcggctat tagctgttcg aattcctcca
     4321 tcgtaccaaa gataggatcg atggctgtgt aatcagagat atcataaccg ttgtcttttt
     4381 gagggcttgg ataaataggg tttagccaaa tcatgtcgat gcctagatca ttcagatacg
     4441 ggagtttttc tttgattcct tttaaatctc caattccatc accattactg tctttgtaag
     4501 atttgggata gatttggtaa atgactttgt ctttaaatgt catggatatt tacctccatt
     4561 caactgattt attacctacc acaacttata tatacaagtt atcatttatt attataactg
     4621 aaccgaaagc gtttgtaaaa tttatcttta tacattctaa gaggtctgtt gcccaataaa
     4681 aaacaagaga tcagacatgt atttgtctat cctcttgttt aaaatagcaa aatattcttg
     4741 tttcttctat aaatggtgtt caaaagcttt tatctacggc aatcagtctt ctcaaatgta
     4801 aaacatgagg agctaatttt atttgagtat tctttttgct cgatgcagga cggctttttt
     4861 acaacctctg attcatggtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca
     4921 aaatatgaaa aactattttg ccgtttgttt ctctaatact caggtcaaac cgctgatttc
     4981 acaacctctg tttttattca ttcgatttca acgcatcaat catatcgata tgctttaatt
     5041 tgaaatgggt aacaatcatc acaataaccg taaatacgat agttaatcct gctgataaga
     5101 cataagccca ccatgtgatc actaatggaa agatgacatt ctccatagac gcttgttgca
     5161 agatgaagtc agtcaatata tatcctacac caaatcctcc gatgattcct aaaagagtaa
     5221 agatgatatt ttctcgaaca atgtacatcg tgacttcttt atcaaaaaat ccaaggactt
     5281 ttattgtaga taattcgcgt acacgctcgg aaatattgat attagttaga ttatacaaga
     5341 cgataaaagc cagcagtcct gataatacga caaagattaa gacaaccgag tctaagttcg
     5401 ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa agacgtattt gtcacagcgt
     5461 ctgtggcaag cagtttctcg gaaagcattt cttcttcttt tgttgtcatc ttatctgtac
     5521 gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac tgaagcataa tattcagggg
     5581 acatataaat aaagctacct agatagtttt tcacgatttc ttttacttcg actttccatt
     5641 ctttttgatc ttcatcataa agcgaaatat gatctcctgt agataagtca aatagttctg
     5701 ctactttttg cgtaatagcg attcctgttt taggcaagga gcgtgggttc tcttcaagat
     5761 cagttaaatg gataaaacga tccaaatcag ctgttttact tggtacgatc actgtcagac
     5821 tttgcgaagc atgtcctttt tttctcaatt ctacggtttg agtatgaata gctaggatct
     5881 catttacttt ttcttgcttc tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg
     5941 atgaatccaa tgtaacgatt gcttgatagt caatgatcgg tccaaattgt ttggctgaaa
     6001 cagaacttag agaatctttc aatcccacgc ctgcaaccat cagcccggca catccagcaa
     6061 ttccgataat cgccataagc attcttgact tatatcggaa gagatttcgg taactgattt
     6121 tttgattaaa gctaaggcgt gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc
     6181 ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg ttctcttaaa tctttgaata
     6241 aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg
     6301 gcggccagtc ataaaaaata agcgcactcc caatatcata ccgctcgtta gacagctcaa
     6361 aaatgatcct tggcaaaaga ttcgttccta atatcgttcc taaaacaata cctatccctg
     6421 aggctagact tgcatagatt gcatattttt ttgcgatttc aactttcgta taacctaacg
     6481 cctttaatgt cccgatttcc ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg
     6541 tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac attggcaatc gcagcgatac
     6601 gatcagacag atcaccaaat tcttgaaaac cgacattttc tgaacgttca ttgtataggt
     6661 atgatggttt agttaaattt tcaatttttt ctgcatttcg attcaattct tcctctgcag
     6721 aggccagttc acgttctttc tcaactaatt gcctttcttg ttcagcaagt tgagcttctt
     6781 gttgagataa tgtttgaaca gcgattgttc gttgcgcttc aggaagctgc tggatagttg
     6841 ctgactgttg cgctaactgt tttttagcat cttctaaccg tgttcttgct tgcgttgttt
     6901 gtgctttgcc atccgctatt ttttgctgtg ccggttctaa ttcttcgttg gccgattttt
     6961 tcagctcttc taagcgttct tcgggtcgat cttgtaaata cttttctgcc ttttcttgat
     7021 tttgctccat tttatctttg taagctttgc tgtacgtatt caaattcttt acgtttgtaa
     7081 aatcaagata taagacagaa taaatatctg ttttaaaaac ggattcaggt aaatagacaa
     7141 aataatcgac gctaccattt ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca
     7201 atggagaacg gacaaagccc gtgatggtat actctttttc tttcaattca tctgaatcaa
     7261 tctgataaat atcaccgatt ttataccctt catcttccgc tttttcatcc aatacaagct
     7321 ggtttggttt ttccgggagt tttccatcag ttaattgtag cttgttctgc ttgttatttt
     7381 tgttataaga aaatatttga atcacttcgt ttttgccagt attggaataa tagatctgtt
     7441 ttcctgtttc tacttttgca tctgggatct tttctgcttg agcaatatct ttttgtgtca
     7501 agccaccagt agaaacgatt tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca
     7561 tcgttttttg catgacaggt ccagcagttt tgacacctac aaataaaagt gttcctagca
     7621 tgacaataag gatgattgca atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg
     7681 ctttaagata tgttcttgtt ttcataaaat ccccctacca tacaatctca tcaatggatg
     7741 aaggatgatc atttaattca acagacctta ctttagcatc attaatgtga atgactctat
     7801 cagcgatggg cgctaaagca gaattatgtg taatgatcaa aacggtattg ccgtgttgtc
     7861 tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg
     7921 tcggttcatc acataaaaga agtttgggat ttttagctag tgcacgagca attgaaaccc
     7981 tctgctgttc gcctcctgat aattgggacg gaaagttatt caatcgatga gctaggccca
     8041 cttgcctcaa tacctctaca ggatcaagcg catcaggaga aacttccgtt gccaattcca
     8101 cattttcttt tgctgttaga ttaggaacga gattgtagaa ttggaaaacg aaccctacat
     8161 ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa
     8221 taatgatctg accttcatca ggtgtatcca tcccacctaa aatattaaga atcgttgact
     8281 ttcctgctcc acttggacca agtataatca ctaattcgcc tttttcaatt gaaaaagaaa
     8341 tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta tcgtttgtat tcatttttta
     8401 cttcgacata cttcactctg attcctccat tttcacgata tttctcgcta tatctatgca
     8461 tcgaatgcgt tttctcacac ttatttattt taccactgaa tttctctaga ctaaagaaag
     8521 gaatccttta atttagttga caaataaacg atttatgatt tacttacata aaaaacattg
     8581 ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa atatgatttt tgatttatca
     8641 aaaactgctt ttgtcgcgat cgacatgcaa aaagagatcg taaataacgg tacactctct
     8701 ccacacactg cagcatctat tctgacaaac aatgatttgt tagtaagaac attgaaacgt
     8761 accgctgcct tgaaagtttt agtgaacgta gacatctcca ctttccctta tttgagtcaa
     8821 caaactgata tgggtggaat gactgcttct gtacctccag aattcactga tttacttttg
     8881 aaggattctt tgaaagatac agataatatg ttgactatca caaaatacaa tccaagtgct
     8941 ttttttggta cttctcttga tcttcaactt cgccggcgtg gaatcgaaac tatcatttta
     9001 agcggtgtag cgacaacaaa cggtgtatat gctactgcat tagatgcctt tcaacacggg
     9061 taccatatcg tgcttgctga agatgcttgc agtgatcgtg acaaagaatc ccatcaattg
     9121 ttcataaaaa agatatttcc gaaaactgca cgagtacgct caacgaaaca aataatcgaa
     9181 gctatccaac aaagcaaata agtactggca cacgtgattt tcatttactt ctttttgatt
     9241 tcatgggaaa ataaccacag gatttagata ggagtatgaa catggtgaaa aaaaagatca
     9301 caatcaaaga agtcgctaaa ctttccggcg tttcaatcac aactgtctcc ttgatattaa
     9361 atggaaatac ggagaaattc agtccgaaaa ctgttcagaa agttttagct gccaaagaag
     9421 aattaggcta tcagcctgac tattttgcta gacaaatgat taccaaggaa acgaaaacaa
     9481 ttggtgtact cgttcctgat atcacgaatc cattcttcag tacactgatg cgaggaattg
     9541 aggatatctt gtataagcag aatttcgtca cgatattatg taatgctgat tctgatcatc
     9601 aaaaagaaat cgaatatcta gccgagctca cacgtcgagg tgtggatgga ttcattatcg
     9661 cgacttctgc tgtctctaca gatgcaatca acgaaaattt gaaaaaacaa ggccggccct
     9721 tcattgtttt ggatcagaaa aaatctgaag gatttagtga tgccgtacga acagacgatt
     9781 ttcgcggtgg ttacctagcg ggcatgcact tgcttagtct aggacatcaa acgatcgccc
     9841 ttgtctatcc tgaaaatcca ccggaaaatg tccacgctcg gattgagggg tttaaatctg
     9901 ctcttgatgt ttaccaaatc cctcacgacc aactgatcct cttacctaca cagttctcaa
     9961 aacaaggtgg ctaccagatc acagcagaac ttcttgattc tgctgcaact ggtgtgtttg
    10021 ctttaaatga tgaattagct tttggattat atcgcggatt agaggaagct ggaaaaagca
    10081 tccctgaaga ctacagcatt attggctatg ataatataga tatgtgcgag tatatcaagc
    10141 cgaaactgac aacgatcgca caaccaatct tcgaattggg acaaacatct gccaagttat
    10201 tgttagatcg gatccaattc ccagaaaaag aatgggaaga aaaaagatta cctgtacgat
    10261 tagaaaaaag attttcaact gcacccttga aatagctttc aaggtgtgct atatttttat
    10321 cgaactatta aaacgtttta ctaaaacgtt ttaaaaaata attttcccat caaaataaat
    10381 gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta ttaatatgga
    10441 cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg cgcatgagat
    10501 ttttcacgct ggcggaggta aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc
    10561 acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt tattagatct
    10621 actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg ccacaaccgg
    10681 acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc atgcaggggc
    10741 aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta caaacagtga
    10801 ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt tttctattgc
    10861 aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac
    10921 tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag aaatcattac
    10981 aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac tccatgaatt
    11041 aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc atacagaaaa
    11101 agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag ccgcaggtga
    11161 tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact tgaaagaagc
    11221 gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc
    11281 tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat gagaggaaga
    11341 aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat
    11401 ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct ttggtactga
    11461 aaaaatagat ttggctgtct caaacggtct tccaagtttt ttatctgttt tagaaaacgt
    11521 tttagaagaa cttgaggtac aatcgattga attggcggaa gaaataaaga caatgaaccc
    11581 ggaaatattg gaaaaaatca aattattgtt accagataca ccgattagct ttatcccaca
    11641 tgaagaaatg aaaaaagagt tgaataactg tcatgcattt attcgtacag gcgaaatgac
    11701 accttacgcc aatattatat tagtgagtgg agtggtcttc taatggaaac gacaatgaat
    11761 cctgaacaga tggtactgtc cgagcagatt catatgctga ttgaacagtt gggaggaaaa
    11821 gacaatattg attctgtcct tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag
    11881 ccagtagcca atgcagaaaa attaaaacag caaatccctt ccattaagta tttcatttta
    11941 ctcgatagat atgcagaaat aatcatggat cagggattgg ctcctgctta caaggaattg
    12001 acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa atccgatcga aaaactattt
    12061 ggtttgatat cggaaatctt tacgccgatc attccgctgt ttgccggttc tggtatatta
    12121 aaaggtctgg ttgttttagc gactacgatt ggctggctta gtgaaacaag cggaacttat
    12181 catattttgt ctgctgcgtc gaatgccgtc ttttattttc tgccaatctt tcttgctttt
    12241 tctgctgcca aaacgtttaa agtgaatgga tatattgcgg ctgtcattgc tgcagcactg
    12301 attgaaccga atctgacctc tttactttca gacagtggca aagcagctac tgattttctg
    12361 gggattcctg tagtactgat gcagtatacg tctactgtca tgccggcgat tttaggtatt
    12421 ttcttttatt cttttgttga aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta
    12481 gtatttgtac ctttactttc attagttatc acggttcctt tgacgttaat ggtattcggg
    12541 cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa ttacttggat gatggatatt
    12601 aacggaccga tcagtggcgc aatcatcgct ggtggttgga atattttggt aatatttggt
    12661 atccaatggg ctgtaaaccc tgtcatgatc agtaatatct ctgcttatgg gttcgaccga
    12721 atcgttccgt tgacaggagc agccaatttt ggaatggctg gtgcagcttt aggtgtattt
    12781 ctgcgaagta aacgttcaaa aacccgttcg atctccggtt ctgcttttgc ttcgatttta
    12841 cttgcgggag tgaccgaacc aactgtttac ggaattgcga tcccattgaa aaaaccattt
    12901 gttgctgctt gcattggtgc agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa
    12961 gcgatcgctt ttgtatttgg tagtctgacc acattgcctg cattcatttc cggtactttt
    13021 ttctggtact tagcaggact ggctgttagt ctagtcgtag caatgatcac tactctcgtt
    13081 agcgggtttg atgaagacct aatgtcttat gaatagttaa tgaaaaagac tgaagtatga
    13141 gatgtttatc acatatttca gtctttttct ttttaggatc attattttta taaatttttg
    13201 tgatactttc caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt
    13261 ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat
    13321 gctcgttgct tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa
    13381 aaagcttttc gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag
    13441 aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc
    13501 tagctgctac ttggctgatt gctccgtctg ttttgtcgac caataccgta tttgccgtag
    13561 ataatcaaat agctgatcaa acagtcacaa cagatacgac tacatccagc tcagctacta
    13621 gtgaaacaac ggattctttt acagaagacc caactggaac aactaactca acaacaggat
    13681 cgaccgagcc aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga
    13741 catattccct tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag
    13801 cggattcccc tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat
    13861 tagcggaaac gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag
    13921 tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga
    13981 acaatttatc tattactgca gaatttaaag ctggaccgga tacttcaaca gagaaaccaa
    14041 ctgatcctag tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg
    14101 tgggtaataa caacaataat aatggttcaa ataattcaaa caataataac aataattcga
    14161 acagcgggaa ttcttcgaat caaacaaacc catctggatc gaataacaat ggaaatactc
    14221 agagagaacc aaaccatcca aacaatggtt catcgaatgg cggaacgaac aatgcttcat
    14281 ctcgtccaaa tacagcaaat caaagcggta tagagaatcc atcttctgct tcttctgatt
    14341 ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa tacaacgaat gtccaacaag
    14401 caatcgttcg agaagcattc aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc
    14461 caaatacttt tgactgttct ggtttgactt attacgtata tatgaaagca accggtcatt
    14521 atatcggtgg ctggacaggc gaacagcaat atgcaggtac gcagattcca gtaagtcagg
    14581 cccaacctgg cgacctcgtc ttctggggac cttcttccgg tgttactcac catgtaggta
    14641 tctatattgg gaacggacaa tttatccatg cgcctcaacc aggagataaa gttcgagtaa
    14701 cttctatttc agatttcacg cctgattttg ctgtccgagt aaacttagca ggtttacctg
    14761 ctgcttcagg ttcgctgaca ggcggctcat ctattttaga tggattagat ggctcgttcc
    14821 atttcagtca aaaccaaaca actgatcagt tcttgaaaaa gattgcagat gatgcacagg
    14881 aaatcgggca aaaagaagga atctacgcat cagtcatgat ggctcaagct atccttgaaa
    14941 gtggatctgg aaacagctta ttatcaagcg aaccaaatca taaccttttt ggtatcaaag
    15001 gatcatataa aggaagcagc gtgaccttca atacacttga acaagatagt tccggacaaa
    15061 gctaccaaat ccgcgcacaa ttccgtaaat atccttctta caaggaaagt ttggaagact
    15121 atgccgactt gatcaaaaat ggactgactg gcaatcctga tttctacaaa ccaacttgga
    15181 agtcggaaac aaaagactac aaagaagcaa ccaaatattt ggaaggacgt tatgctacag
    15241 atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg
    15301 atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt
    15361 ctaagcgatt cgatgtgcca atcaaatgga caacagatgg cgtgccaatc acaggattag
    15421 aattttcaac gataaaacgc gtcaacaacg cttcttccat cttccgtgtc gcttcaatct
    15481 gggatttgtg gaacaatttt acggcaagac agataccaga aacaactact cgtacagtta
    15541 aagctgcaac tgttcctgta ctaaccttgt taaggttaga caaatcattc tggcttgatc
    15601 tgcctttaaa gaaatagaat aaattaaaaa ggggatgtga cactgtcaca ctcccttttt
    15661 aattt
//
LOCUS       DAYYCM010000063        15291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000063 DAYYCM010000000
VERSION     DAYYCM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.064"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHAY1_002177"
     CDS             complement(93..1136)
                     /locus_tag="ACHAY1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8594140.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHAY1_002178"
     CDS             complement(1513..2478)
                     /locus_tag="ACHAY1_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8594141.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHAY1_002179"
     CDS             2712..3557
                     /locus_tag="ACHAY1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8594142.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHAY1_002180"
     CDS             3748..4935
                     /locus_tag="ACHAY1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8594143.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHAY1_002181"
     CDS             4941..5297
                     /locus_tag="ACHAY1_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8594144.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHAY1_002182"
     CDS             5299..5637
                     /locus_tag="ACHAY1_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8594145.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHAY1_002183"
     CDS             6032..7066
                     /locus_tag="ACHAY1_002183"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8594146.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHAY1_002184"
     CDS             7059..7745
                     /locus_tag="ACHAY1_002184"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8594147.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHAY1_002185"
     CDS             7769..8617
                     /locus_tag="ACHAY1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8594148.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHAY1_002186"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHAY1_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8594149.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHAY1_002187"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHAY1_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8594150.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHAY1_002188"
     CDS             10883..12118
                     /locus_tag="ACHAY1_002188"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8594151.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHAY1_002189"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHAY1_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8594152.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHAY1_002190"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHAY1_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8594153.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHAY1_002191"
     CDS             complement(14069..15211)
                     /locus_tag="ACHAY1_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594154.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4997 a         2848 c         3262 g         4184 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat c
//
LOCUS       DAYYCM010000064        15223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000064 DAYYCM010000000
VERSION     DAYYCM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.065"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(598..1164)
                     /locus_tag="ACHAY1_002192"
     CDS             complement(598..1164)
                     /locus_tag="ACHAY1_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8594155.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1164..2120)
                     /locus_tag="ACHAY1_002193"
     CDS             complement(1164..2120)
                     /locus_tag="ACHAY1_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8594156.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2120..2776)
                     /locus_tag="ACHAY1_002194"
     CDS             complement(2120..2776)
                     /locus_tag="ACHAY1_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8594157.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3146..5164
                     /locus_tag="ACHAY1_002195"
     CDS             3146..5164
                     /locus_tag="ACHAY1_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8594158.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5234..5440
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5591..8005
                     /gene="leuS"
                     /locus_tag="ACHAY1_002196"
     CDS             5591..8005
                     /gene="leuS"
                     /locus_tag="ACHAY1_002196"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8594159.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8353..9906
                     /locus_tag="ACHAY1_002197"
     CDS             8353..9906
                     /locus_tag="ACHAY1_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8594160.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10287..12071)
                     /locus_tag="ACHAY1_002198"
     CDS             complement(10287..12071)
                     /locus_tag="ACHAY1_002198"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594161.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12086..13807)
                     /locus_tag="ACHAY1_002199"
     CDS             complement(12086..13807)
                     /locus_tag="ACHAY1_002199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594162.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13907..14668
                     /locus_tag="ACHAY1_002200"
     CDS             13907..14668
                     /locus_tag="ACHAY1_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8594163.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4961 a         2783 c         2993 g         4486 t
ORIGIN      
        1 gaatactgtt tctttttttg cagttacagg ttgtttattt tcggaaaaag ctaatatagc
       61 taagattgaa acatccatct ggaatttatc ttttttactg ctttgatcat aaaagtctgc
      121 atattcatca ctgatatttt ttctaataaa ttcttccatc ataaaaatca cctcaaaacg
      181 attatacgaa cttacgttct tgttgtaaag cgatatttga ggggcaaaaa aggggcataa
      241 tttgactgtt attaagttag ctattgatat atgaatgaca tacaaaaata taaaatcttt
      301 taaaaatgct tatatatcaa tgtttttgcc attcttttat atatctgtta tatgtcaaaa
      361 aaatgtgttc aatttcaatt tgtatttgaa taaagaggtt gtgaaaaagc tgtcctgcat
      421 caagtaataa gaacagccaa tcaaaaacag cttctcatgt ttttgatttg gttggactta
      481 ttaccgcaga tataagcttt tgagcaccgt gaataaagag gttatgaaat caacgctcta
      541 acctgagtat tagagaaaaa aacgcaaaac atatcattta tgttttgcgt tttttattta
      601 tctttttttc tcaatacaat ataaaatagg cggctggttc ttttggttga taaaccgata
      661 attcagcaca ttatatgttt cctgtggcag tttggaacaa aaagaatcaa ccatatccag
      721 ttctttttct ccaccttcat gaccatagta gacgacaagg atcaatcgtc cgcgaggcac
      781 aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc tctggcatag tgatgattgc
      841 tttatcactt tttgggagat accctagatt gaaaatccct gctttgagat tttgttgttc
      901 agctatcact tctcctaacg tttcgtggcc ttgtagaaac aatgagacac gttcttctaa
      961 ctgacgctgt tctagccttt cacgagtagc atcgattgct tgttgctgga tatcaaaact
     1021 atagacatga ccagtttttc cgatgtgttc tgctagaaaa accgtatcgt ggccattgcc
     1081 catcgttgca tctacgacgt gatcccctgg ttgcaagatt tcttgcagta atgtatggct
     1141 gaaatgcaaa gcagtttgca acatcagacg ctcacctttt tccgtacatc atattttcct
     1201 tggaagctat ctctgcgaag aagttcttca tcaattgcat tcaagacttc ccattttttc
     1261 aagctccaca ttggtccaat caaggaatcc cacggcgcat cccctgtcaa acgatggatg
     1321 atgatctctt ttggtatcat ttccagctgg tcgcaaatga cattgacata gtcaggacgg
     1381 ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca ttctcgtttt gcgcatcaga
     1441 tgcatcaaat ggagcttgat tccttggata tctgaatcta aaatggtgcg tctgacattt
     1501 tctttcatca tttctaaact ttcgcctggt aatccgttga tcaaatgcgt acaaacacgg
     1561 atattatgtt tgcgtaattt agccacgcca tctaaataag tttgataatc atgtgctcgg
     1621 ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc caagttctac ccataaatac
     1681 atccgctcgt tcagttcagc aaggtaatca actacttcat ctggcagaca atccggtcgt
     1741 gtaccgactg ataatcctac gacacctggt aaattgacta cttgctcaaa tcgttctttg
     1801 attatttcaa ctggcgcatg ggtgtttgta aaattttgga aataaacgat gtattgttgg
     1861 acatgaggcc atttttgatg catcatgtcg atttctttct ggaattggat tggaagaggg
     1921 tcttcaggtg caacgatcat gtctcccgaa ccagatacac tgcaaaaagt acaaccgcca
     1981 tgggcaacag tcccatctcg attcggacag tcaaatcctc catcgatcgg caccttgaaa
     2041 attttcccgc caaactcatt tcgtaaagcg taattccaag aatgatatct tttatttcct
     2101 tctgaataag gaaaagtcat taaaagtcct tctttctgta agagttatct atttgttttc
     2161 ttttttctct atacactgga taagtcaggc acagccaaga aagactgagt aagtaaccgc
     2221 cgatgatatc agtaggaaaa tgaacgccca gataaatgcg gcttatcccg acacttacta
     2281 taataaacat catcagcaat tgaagcaatg tgcgtaaggt tcttttttga acaaaagctg
     2341 gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc tgctgcatgt ccactaggga
     2401 aactgtaact tgtttcaact actaagtgcg ccaatgaagg acgctctcgc atgaaaaata
     2461 gtttgatcac cgggttgatg attccagcaa ttacaataaa tccagaaacg aaccaaaatg
     2521 tttctgccct ttttttctta taccacatcc ataaagccgc acaagcaaat aaaatgatga
     2581 cggtaagagg attaccgaat ttagtcataa agagttggta gctgttcaga tcaggataag
     2641 aagctctgac aatgcctgta aaccactgat cgaaaccaga aagccacgac agatggaact
     2701 ttacgatcca gcccaaaatg aaaaacagga caagaaagaa gctaccagcc acctgaaatg
     2761 atgttttata tttcatacaa ttcctccttt gaagcaatga cttattatag catttttggt
     2821 ccagcgaaaa aaacggtaaa gagataaaac caatgtatga ttcctctttg aataaccaat
     2881 attcttataa aaaaaataac tgtaatcgga aagacatttt ttggtaatgc aaatgaaatg
     2941 ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa caagaacgta gacgtaggaa
     3001 gaacttgaaa atgaacagaa gttgttcatt acggtaaatt acaatatgat tacaaaaaac
     3061 aacaaagctc ttaaataact tttaataaat attaagagaa gttataatta ttaataatag
     3121 ataaattgaa gttggaggat agaagatgga aaacattgca agaaaagaac ggagacgttt
     3181 agaacagacc aaacgttttc gaaaagcaaa acgaggggca gcaatcgtgg gaactgcgat
     3241 ggtaggctgt tctgtcgctg ccccattagt acagccggtt cctgttcaag cggatgaaac
     3301 acctgttcag ttcggcgcaa gaatcaatac agcggcattt atcacagaaa ttgctactta
     3361 tgctcaacca atcgctcaag ccaacgatct atatgcatca gtgatgatcg cacaagcagt
     3421 tgtagaaagc ggttggggca gtagtgcgtt atctcaagca ccttattata atctttttgg
     3481 gataaaaggc agctatcaag gacaaaccgt ctacatggat acgcttgaat ttttaaataa
     3541 tcaatgggta aataaaaagg agcctttccg acaatatcct tcttttgctg aatctttcaa
     3601 tgacaatgcc tatgtactta gaaatacttc atttggcaac ggttattact atgccggtac
     3661 ttggaagagc aacacgaaat cctatacaga tgcgacagct tgtttgacag ggcgttatgc
     3721 gactgatcct ggttacgcag gcaaattaaa taatatcatc gcaacttacg gattgaccca
     3781 atatgataca ccagctagcg gaaacgcagg cggtggagcg actacaggtg gcaataatac
     3841 tggaagtgca ggtagtactg gcggttcaag caattctggt aatacaaact caggttcagc
     3901 aacttcgacc acttatactg tccaatctgg tgattctgtt tggggaatct caaataaatt
     3961 cggtatcaca atggatcaat tgatccaatg gaacaacatc caaaacaatt tcatttatcc
     4021 aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc tcttcgacga ataattccgg
     4081 taacaatacc aattcttcag gaaatgctgg tacatcaaac ggaggacaaa caactggtgc
     4141 gaaatacact gttcaatctg gtgattctgt ttggaagatc tcaaatgatc acggaattac
     4201 gatggctcaa ttgattgaat ggaacaatat caagaacaat ttcgtttatc caggacaaca
     4261 gctaatcgta tcaaatggcg gttcttctaa tgctggagca aatactggct caaatactgg
     4321 gaatacaagt ggctctagtt cgtccagctc aagttcttct aatacagctt caggaacgaa
     4381 atatacagtt aaagccggag actctgtttg gagcgtctca aataaatacg gaattacgat
     4441 ggctcagcta atccaatgga acaatatcca aaataacttt atctacccag gacaacaact
     4501 gactgtttca aacagtggat caacaaacag cacgtcaggt aatacaggtt cgtcaaccaa
     4561 tacaaataac agcagttcag ttaatactgg cagcagttcg tctcaaggca cttataccgt
     4621 gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac ggcatcacga tggatcagtt
     4681 aatcgaatgg aataatatca aaaacaactt catttatcca ggacagcaat tgatcgttaa
     4741 aggtggaact tcggcaaata caaatacagg ttcaactact tctgcagcga aaccaaatac
     4801 accgaatacg tctgctacga caagtacatc atccactggt aatacgatgt ataccgtgaa
     4861 agcgggcgag tcagtttgga gcgtagcgaa caaacatcat atcacgatgg atcagttaat
     4921 cgaatggaat aatatcaaaa ataatttcat ctatccagga cagcaagtga ttgtgaagaa
     4981 aggttcatct caatcaacaa accaacaatc agttactggt tcgaaaacct atactgtgaa
     5041 atctggagaa tctgtttgga gtgttgctga cagtcatgga attacaatgg ctcaactaat
     5101 cgaatggaac aatattaaga acaactttat ttatccaggt caaacgttga ttgtagctaa
     5161 ataaaatagc tgttcgaaga aataacgatt gaattccaca aaaatatttg ttactataag
     5221 gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa aagactgaag tttcagagag
     5281 cgcatggctg gtgagaatgt gcacggatgc ttttgaactc gcctttgagt tgcccttgct
     5341 gaatacccga gtaagcaagc ggcggtgacg gccgttacaa cgttcgaggc cttttcacta
     5401 aggtaaaatt aggtggtacc gcgtagatta cgtcctataa aaagagcaat ctttttatag
     5461 gattttttta tttcttttgt gaagtttgag aaaacaaagc aaaaggaaat aaaaataaac
     5521 agattctgcc ggttttttcg ttatatttat aatagaaaca agtgttttca atagaacagg
     5581 aggacagcaa atgagttaca atcataaaga aattgagaaa aaatggcaaa aatactgggc
     5641 aaaaacgaat actttcaaca cccatgatga tcctgaaaag ccaaaatttt acgcactgga
     5701 tatgtttccc tatccatccg gtcaagggct acatgtaggg catcctgaag gttatacggc
     5761 aacggatatc ctttcacgtg tgaaaagaag tcaaggatac aacgtgctgc atccaatggg
     5821 gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta gatacaggaa acgatccagc
     5881 tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa atcaattctt taggatttag
     5941 ttatgactgg aatcgcgaag tgaatacaac tgatccggag tattataaat ggacacaatg
     6001 gatctttacg aagctttatg aaaaaggtct agcttatgaa gcagaagtag cggtaaactg
     6061 ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg atcgatggga aaagtgaacg
     6121 tggtggctat gatgtagtca gaaaaccaat gcgccaatgg atgctgaaaa tcactgctta
     6181 tgctgatcgc ttattggatg atctagaatt agttgattgg ccagaaagca ttaaagaaat
     6241 gcaaagaaat tggatcggtc gttcagtagg agcaaatatt gaatttaaag tggctggtac
     6301 ggataaatcc tataccgtat ttacgacacg accagatact ctattcggtg caacttactc
     6361 ggttcttgca cctgaactag atcttgttag agaaattacg acccctgaac aaaaagctgc
     6421 tgtagaagct tatatcgaag agacagctaa aaaatcagat ctaaagagaa cggatcttgc
     6481 aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc aatccagtta atggcaagga
     6541 aatcccaatc tggatcgccg attatgtatt atcttcatac ggcacaggag ccatcatggc
     6601 tgttccagca catgatgaac gtgactttga attcgctcaa actttcggac tagaaattct
     6661 tccagttatc gaaggtggag atgttgaaaa agctgcttac acagaagatg gtacgcacat
     6721 caactctgaa tttctagacg gcatgaacaa acaagaagca atcgataaaa tgaatgaatg
     6781 gctagaagaa aatggcgtag ggaaaaaaga agtcagctat cgcttacgtg actggctatt
     6841 ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc cattgggagg acggaacagt
     6901 cacagctgtc ccagaagaag agctaccatt acggttgcct aagacaaaaa acatcaagcc
     6961 aagcggtact ggagaatctc cattagcaaa tatcgaggag tgggtcaatg tcgttgatcc
     7021 tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg ccacaatggg caggaagctc
     7081 atggtattac ctgcgttata tcgaccctca taacaaaaaa gaactagcag attatgaaaa
     7141 gttgaagcgt tggctaccag ttgacatcta cattggtgga gcagaacatg cggtacttca
     7201 tttactatat gcacgattct ggcataaatt cttatacgat atcggtgttg ttcctacaaa
     7261 agaaccattc caaaaactat acaatcaagg aatgatctta ggcgaaaaca acgaaaaaat
     7321 gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg gtcgaaactt acggtgcaga
     7381 tacactgcgc atgtacgaaa tgttcatggg gccattagat gcttcgattg cttggagcga
     7441 aaatggctta gaaggaagcc gtaaattctt ggatcgtgta tggcgtttga tcgttgatga
     7501 gaacaataaa atgcgtgacc gtatcactac attgaatgat ggcaagcttg ataaagtgta
     7561 tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat cttcatttca atacagcaat
     7621 ctcccaattg atggtcttta tcaatgaagc ctataaagtg gacgctctgc cttatgaata
     7681 tatcgaagga tttgttcaac tactcgcacc gattgctcct cacatgggtg aagaactatg
     7741 ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca tggccaacat acgatgaagc
     7801 agcacttgtt gaagacgaag tagaagttgt tttccaagta aacggtaaag ttcgtgcgaa
     7861 aagcaatgtt ccccgagact taggaaaaga tgagctagaa aaagcagcat tagccaatga
     7921 aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa gttattgcgg taccaaacaa
     7981 gttagtcaat atcgttgcta attaattggc tctttgtcaa taaggactga tgagttgtgc
     8041 aaaatcaaat ctgggccaga atgaacttca ttctgtctca gattttttgt tatttcaaat
     8101 tgattaattg attgatcaaa aaggttttga ttttcatgtt ctcaaacacg ttcaatgtct
     8161 ttttcttctg aatggtcttg taagagatct ctgtaatcat caaatttttg tctattaatc
     8221 ttagtatttt ttgatgtata aactacctga aattgagagt acataccaaa ttcataaatt
     8281 ttttattagt gggactagtg attgtatgga ttttgacgca taatgaatga agtatgagaa
     8341 aaggagagac aaatggatca agaactattt attatcgaag tattatcagc taaacaaaaa
     8401 attatcgaga aagcgaaaaa aagagcgaaa agaataatgt tcaagctttt aagcttagta
     8461 tttttctctg gtttgctttt tttcggagct gtatcgttcc tatatcgcca tatgagtgca
     8521 gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt tgtatcactt ttcgtgggga
     8581 gttggcggaa taacaattgt tgtgcttatt gtttttcttc tacttatttg gctccgttac
     8641 catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa gagcacaaca gtttttacat
     8701 aataaaccaa cttctcaaga tgatgactat ctttattttg agaagacttc gacgagtcaa
     8761 gcttttcgga tacaaaaaag tgattgccag ttattgctga gatcacttga tgatttacct
     8821 atctacttag gcgaaagcaa atcaaaattc ggaatgaaca cattccatat ttttttacta
     8881 gaaaacgaac ccagatggga aaaaagccag tcttcgcctg gtggatacct gacaactaag
     8941 atgagtacat tagtcactgg ttttttcctc attcttttag ctggaacaat ctattatggg
     9001 agatacactc agccaaaaat gtcttctcat ataaatgctg cttctcacat gacaatgaac
     9061 tcaactaacg atacgcatat acaaaagaca aatgaagggt tgttagctga tcctaagcaa
     9121 aaggtaaatt tgacaacaca aaagataaat gatctcaaac ttgatacaga gacgcactct
     9181 ctctatatga caacagatag tggtgctaat tgggtatttg tgccgattga ttcgcaatgg
     9241 ttgcgttttg gcgaatacac cctgacaacg gggctgattc ctgaaggtta ttggatggac
     9301 aacacatatg atatttccgc cgatttctca tggtttatct attcttctgg ggatgatgta
     9361 cgtctgctga cttcaagaga taatggtaaa acatggcaaa cgtctattat cacccaatca
     9421 gttcagccga tacgttatcg aaaagcggta ttttatggca acgatggtct atttatctgt
     9481 acatcagcca gtgggatgtc tgcagaaact ttgtacattt acactactat ggatggcgga
     9541 atcacttgga taaaaagtgg aagtacaatg atcaatcagc cagtacaaaa tgtcagcttc
     9601 gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt tgttttatac aaacaacgga
     9661 ggacgctcat tcaaagaatc agttctgtcg attccggaaa actatcagac aggggggata
     9721 gatctttttc aatcaccaaa tgaagttact caaataagcg cgaatacgtt agagacaaaa
     9781 ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa tgtttgcctg cctcttccgt
     9841 tcaactgata acggggaaac atggcaattc gttcagcaac tatcgcaagt agaacaagga
     9901 gattaaagaa gagagattgt gagcctgacg ttcagcaccg agtcacaaga aacaaattca
     9961 aaaaacagct tctcatattt gttgaatagt gtaactcgtg attcggagtt gacaagagca
    10021 acaagaagcg attttgaagt atagcttctc gtattttaca agatcgtgtc ttattgccga
    10081 taggttgtct cttgaacacc gtttattcgt aaaaagggat tgtgacaagt cttttgtcac
    10141 tacttaccga acgaaactga aggagctatc caaaaagtat cattagtatt attctttgtg
    10201 tttctagtgg caccactagt acatttaaaa aagctatcga tcatggacta atcatacatg
    10261 atcgatagct ttttagataa tgtttgttac gctatctgaa attgactatt ataaagactg
    10321 gcataaagcg ttggtgattg gagtagttca gaatgtgttc ctttttcgac gatatcgcca
    10381 ttcttcatta caaggatcat gtctgcattt tcaatcgttg acaaacggtg tgcaatcaca
    10441 aaacttgtcc tatcttttgt cacagcttcc attgcttgct gaatcaatgc ttctgttcgt
    10501 gtatctacgc tagaagttgc ttcatctaaa ataacgacag gaggattcgc tagaatgact
    10561 cgagcaattg tcaataattg ttgctgtcct tgagataacg tgccgttttc gctagaaatg
    10621 atcgtatcat atccgtctgg cagtgttcga ataaagtgat cacattgtgc gatttcagcc
    10681 gcttcgataa tttgttctct tgtggcatcc attttcccgt aggcgatatt atcagctacc
    10741 gttccttcaa acagccaggt attttgtaaa accatcccaa aagaacgacg tagttctcca
    10801 cgtgaaagct tggtaatatc aagaccgtcg aattcgatct ttcctttatt cagttcataa
    10861 aaacgcatca gcagattgac taaagtcgtt tttcctgcac cagttggacc aacgatcgct
    10921 accgtttgtt ttggctttac tgaaaaatcg acttgattca tcaatatttt atctgatgtg
    10981 taaccaaact gtacattttt gaaagctatt tttcctttcg gttgttctaa aagtcttgca
    11041 tctggaaggt ctggaatttc atctgtttca tctaatatct caaatatcct atcgattgct
    11101 gccattgcgg attggatcga gttgatcaca taagaagcgg tagaaattgg ttcagaaatc
    11161 tgattgatgt actgaagata agcttgtaag aagccgatcg tcacaccacc tgatagaaca
    11221 agcatagcac cgaataccgc acttatgatg aaggcgagtt gattaatgaa acgaatggcc
    11281 ggatagatag cacagttcat aaattgagca cgtttaaaag cgttgtattg ctttttattt
    11341 acttcgtgta tagcagcttc tgcatgagct tgctgattga atgttttgac cactagatta
    11401 ccagctaaga tttcttccat tcgattattt aaaacaccca gttcatactg attctgttca
    11461 gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa ttgaactgcc aacgatcagc
    11521 aataagacga gcaaagtaag cttgctttcg atataaaaca gcatcagacc agcagcagct
    11581 attgttacga cagaagtaaa aaattgattg atacctgtca ataacacttg agaaagttga
    11641 ttgagaccgg tagttgtccg actaatgatg tcacctactt gatgattgtc aaaaaacgac
    11701 atcggtaatg ttttgaattt ttttgtgatt tcttttcgca ccctcaatgt gatcttttcg
    11761 cttaacgctg ccattgtccg ttcctgaata aatgaagtga tgctgcttaa gactgaacag
    11821 ccgatcagca ataagacagg gaacaacaat gcttgtttga tatcactgaa agaaagctgt
    11881 ccaatacctg tttttcttat ttctgataag agatcatcga tcccgattcc catgatcaag
    11941 ggcatggcta cgactaatag gttcccgaat aaactgcata gaaacaatcc gtaaaaatat
    12001 ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag cacgcgtgtt caacgttttt
    12061 ttcttttcca tcgtatccct tctttctata ttccttgtga ttttgcaaac tcctgataag
    12121 tttggcaatt agacagtagt tcttcatgag taccttgtcc aacgattttt ccttcatcta
    12181 aaacaatgat gttatctgct tgttggattg tactgaggcg ttgagcaaca atcacgagag
    12241 ttttctccga catgtgttta tgaagggctg ctcttaatgc tgcatctgtt ttatagtcta
    12301 aagcggagaa actatcatcg aatacataga tctctgctgg tttgatcaac gctcgagcaa
    12361 tacatatccg ctgtttctgg ccccctgaat aattagaacc gccttgagca acaaagctgt
    12421 ccaatccgtc aggaagggtg tccactagtt cttctaattg agcgatcttg atcgctttag
    12481 acaactcttc tgttgaggca tattcatttc ccatctgcaa gttgcttaaa attgtcccgc
    12541 tgaataagaa agccttctga gggacataac tgatgtggga gcggatctgt tcttgagtca
    12601 tttgagtaag aggaatacca ccaagggtta cttttcctat cgtagggtca ctaagtctca
    12661 agagaagttt cgccaaacta ctttttccag atccggtccc gccaacgatc gctgttgttt
    12721 ttcctttagg tattgtgaaa tcaatatctt ctagaactgg atcagagatt tcagtatatt
    12781 taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc aaaggttggt tcagctactg
    12841 ttttgatcgt ctcttcctca atttcgatct ctgcttgcag tatttcttct atccgtcgta
    12901 atgaggcaaa cgatttaggg ataatgacca ataccatagc tgcgatcatc aagtaagcaa
    12961 gtgtcagcat agagtattcg actacagcag tcacgacacc gatttgtaat gctccttctg
    13021 ctacaagatt tccgccaaac cacaaaatag aagcataaac gattcccatg agcaaaaaag
    13081 caattggagt gacccaagca aatattcggt tcactttgat catttgatta gcatagtcag
    13141 caaagctctt attcgttcgc ttttcttcat atgattgatt atcaaaggca cggatcatat
    13201 taatcccagt aaagaattct ctcaatgtga tggtgatccg atccattttt ggctggatac
    13261 tcaaagataa aggtgcccct cgtttcatca gtcgataaac aatccataca taaaaaacaa
    13321 cagagatcaa tgtaacccat gttagtaata gagaataacg aaacatcatg agaatggtga
    13381 aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa taaaacaatc atttgctgga
    13441 cattatcgac atcattcgtc attcttgtca aaagagatcc agaactaaaa cgactgatat
    13501 ccttcattga cagctgttgg aatttacgga aatatttctc ccgcgtttgg aatccaaacc
    13561 tagcggaagc caatgctgaa aagtaactag aggcaactgc agcgattgtt cctagaaacg
    13621 ctacaagcag catttcaact ccaatttttt ttataactga ggcttcgcca cttgctatac
    13681 ctgtgtcgat cagctttgct actaattttg gtacacctaa tgtaccggcg atttctaaac
    13741 agatcatcac aaaggtaagg agagcgagtc ctttgttttt ctgtaagaat gtttttaaga
    13801 tagacatatt attcctcctt ttttcctgat acttttcgat tctatacctt caagtaactt
    13861 gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga gaaaaaatga aaaaaatgta
    13921 tacgattggc caagtatcgg aactatttga tttgccaaaa tcaacgatcc gctattggga
    13981 tgaacaagga ttaatccgtt caagtagaca agaagagaat gattatcgat tgtttgatat
    14041 cgatgatatt tttatgcttt atgatatcgt tttttataga aaattagata taccgatcaa
    14101 acagatgaag aatttatatg gaaaaacatt gacggagtta tatgagacac ttgatgaaac
    14161 agaaagaaga atccataaag aactggtggt gatgaaacaa aaacaaaaag agataaggga
    14221 gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag gaagaatttc ctgtagaaga
    14281 gatccctttt gactgtatga tctcaacgga gtttgaagat attgtggaaa tcaaaaaatt
    14341 tctacctaac tattcaagct ttgggatgat gagtatgcca gcttccagtt cacagacgat
    14401 gtatggattt tttatcgacc ctagtgaagt acatttattt actcaagacg tgatttggga
    14461 gaaaaaagat acggctgttt atcgtcgatt cttattgaaa tccgagatga accatgcgga
    14521 acgtaataat attttagaaa tcagagaaag aatgtatgaa aaaggatgga aaacaggaga
    14581 agtgatcggc cagtatctat tgacgaatac ggatgaaaac aatatcagaa ctgaatatta
    14641 tcatgcatgg atagaaatga aaaaatagga aatatgtgtt taagataact cgaaaatgat
    14701 ggttgtttta gttataaaga aaaattttct ggttcgatta cgggtttaat gtgggggatt
    14761 attagtcgtg gatagataga attttagttt tttagaggaa atatgtcttt aaaaatggaa
    14821 tggttgcaat tcttagcgat atatacgatg taattgtcct cttaaaaaag tatggaaaaa
    14881 taattgtatt gggtgttatg tataattcat ggtgaactga gactaatgag aagaaaaatt
    14941 catattgtta tgagtgagat tgtatcagat gagaatgata aattaaatag gtgcataaaa
    15001 gcattacact taaaatacat tgttgcttac attattacaa tatgttaata aggcaaaaat
    15061 aaatagatgg attgaataaa catatttttt aatcaaatag tccctactgt gactttcctc
    15121 agctatcggt gatactaccg atagcatttt tttgcttata aaataattat aagtatttag
    15181 agatttttct tctaacattc ttcaaacaag aaaaatcaca aag
//
LOCUS       DAYYCM010000065        15137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000065 DAYYCM010000000
VERSION     DAYYCM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.066"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACHAY1_002201"
     CDS             4..2376
                     /locus_tag="ACHAY1_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8594164.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACHAY1_002202"
     CDS             2437..3897
                     /locus_tag="ACHAY1_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8594165.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACHAY1_002203"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACHAY1_002203"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8594166.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACHAY1_002204"
     CDS             6128..6277
                     /locus_tag="ACHAY1_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594167.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACHAY1_002205"
     CDS             6352..6945
                     /locus_tag="ACHAY1_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8594168.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACHAY1_002206"
     CDS             6958..7857
                     /locus_tag="ACHAY1_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8594169.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACHAY1_002207"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACHAY1_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8594170.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACHAY1_002208"
     CDS             8723..8983
                     /locus_tag="ACHAY1_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594171.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACHAY1_002209"
     CDS             9428..9634
                     /locus_tag="ACHAY1_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8594172.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACHAY1_002210"
     CDS             9655..9885
                     /locus_tag="ACHAY1_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594173.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACHAY1_002211"
     CDS             9901..10338
                     /locus_tag="ACHAY1_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594174.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACHAY1_002212"
     CDS             10331..11038
                     /locus_tag="ACHAY1_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594175.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACHAY1_002213"
     CDS             11202..11402
                     /locus_tag="ACHAY1_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594176.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACHAY1_002214"
     CDS             11418..11597
                     /locus_tag="ACHAY1_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594177.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACHAY1_002215"
     CDS             11922..12185
                     /locus_tag="ACHAY1_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8594178.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACHAY1_002216"
     CDS             12179..12529
                     /locus_tag="ACHAY1_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8594179.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACHAY1_002217"
     CDS             complement(12553..13167)
                     /locus_tag="ACHAY1_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594180.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACHAY1_002218"
     CDS             13481..13903
                     /locus_tag="ACHAY1_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594181.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACHAY1_002219"
     CDS             13913..14116
                     /locus_tag="ACHAY1_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594182.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACHAY1_002220"
     CDS             14327..14911
                     /locus_tag="ACHAY1_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594183.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3060 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaa
//
LOCUS       DAYYCM010000066        15024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000066 DAYYCM010000000
VERSION     DAYYCM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.067"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACHAY1_002221"
     CDS             complement(<1..626)
                     /locus_tag="ACHAY1_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8594184.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            complement(1200..2384)
                     /locus_tag="ACHAY1_002222"
     CDS             complement(1200..2384)
                     /locus_tag="ACHAY1_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8594185.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2409..3416)
                     /locus_tag="ACHAY1_002223"
     CDS             complement(2409..3416)
                     /locus_tag="ACHAY1_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8594186.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(3588..3947)
                     /gene="comGG"
                     /locus_tag="ACHAY1_002224"
     CDS             complement(3588..3947)
                     /gene="comGG"
                     /locus_tag="ACHAY1_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8594187.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3919..4344)
                     /gene="comGF"
                     /locus_tag="ACHAY1_002225"
     CDS             complement(3919..4344)
                     /gene="comGF"
                     /locus_tag="ACHAY1_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8594188.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(4307..4654)
                     /locus_tag="ACHAY1_002226"
     CDS             complement(4307..4654)
                     /locus_tag="ACHAY1_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594189.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(4626..5096)
                     /gene="comGD"
                     /locus_tag="ACHAY1_002227"
     CDS             complement(4626..5096)
                     /gene="comGD"
                     /locus_tag="ACHAY1_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8594190.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(5071..5349)
                     /gene="comGC"
                     /locus_tag="ACHAY1_002228"
     CDS             complement(5071..5349)
                     /gene="comGC"
                     /locus_tag="ACHAY1_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8594191.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(5346..6395)
                     /gene="comGB"
                     /locus_tag="ACHAY1_002229"
     CDS             complement(5346..6395)
                     /gene="comGB"
                     /locus_tag="ACHAY1_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8594192.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(6352..7281)
                     /gene="comGA"
                     /locus_tag="ACHAY1_002230"
     CDS             complement(6352..7281)
                     /gene="comGA"
                     /locus_tag="ACHAY1_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8594193.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7413..9167
                     /gene="ade"
                     /locus_tag="ACHAY1_002231"
     CDS             7413..9167
                     /gene="ade"
                     /locus_tag="ACHAY1_002231"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8594194.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="ACHAY1_002232"
     CDS             complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="ACHAY1_002232"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8594195.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9997..10779)
                     /locus_tag="ACHAY1_002233"
     CDS             complement(9997..10779)
                     /locus_tag="ACHAY1_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8594196.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10983..12194)
                     /locus_tag="ACHAY1_002234"
     CDS             complement(10983..12194)
                     /locus_tag="ACHAY1_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594197.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(12187..12924)
                     /locus_tag="ACHAY1_002235"
     CDS             complement(12187..12924)
                     /locus_tag="ACHAY1_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594198.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13062..13496
                     /locus_tag="ACHAY1_002236"
     CDS             13062..13496
                     /locus_tag="ACHAY1_002236"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8594199.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            13489..13833
                     /locus_tag="ACHAY1_002237"
     CDS             13489..13833
                     /locus_tag="ACHAY1_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8594200.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            14013..15023
                     /locus_tag="ACHAY1_002238"
     CDS             14013..15023
                     /locus_tag="ACHAY1_002238"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8594201.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4759 a         3062 c         2550 g         4653 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      721 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgttt tttcgcaaca
      781 aaaaacttga ttttcatacc tgtgctttca cgattcaggc aaagtacagg tggaaaatca
      841 agtttttatt attcgatcaa tgaatgtttc ggatcggttt tctagctaaa cataaagcga
      901 agaataaagt cgacagccca cgaggctgat ctaaaaatgt tttttctttt tacatatgta
      961 atgtccctta taaagatgaa tgaaaataaa atattgggat tttcctgaaa atcaataaat
     1021 aaaatgccag tcttttccct tttgaccact tttccaagca aaattcccta agaagatcct
     1081 gtaaaaaaga agggaaattg aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa
     1141 cacatgcaag aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct
     1201 tagccgcgca gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact
     1261 aatactctta cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc
     1321 aacgctggat cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca
     1381 tgtccgtcat tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt
     1441 gtcacataac cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga
     1501 atatgttctt tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg
     1561 cctagcagac cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat
     1621 ttttccatta aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc
     1681 accccagcta atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct
     1741 gtgatcgata caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg
     1801 ttcaataatt ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat
     1861 tttcttactt tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt
     1921 ggcattgttg agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc
     1981 tttttaaacg ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct
     2041 aattcagcaa tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct
     2101 cctccgtgaa caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc
     2161 agttcaatca actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg
     2221 atttgttcga atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg
     2281 atacgttcga cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat
     2341 ttcaaacttg aactcccagc attgattgca attgttttag acatatgact ctccttctcg
     2401 ttgaatgatt acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc
     2461 ttctctggtt cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct
     2521 tgtggtcctt ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat
     2581 tcatctggca attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt
     2641 tcttcacttt gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc
     2701 ggcttcacat atttcattga ttgctctagc aataaatgat gtgcataact atggccttct
     2761 tcggtacttg ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa
     2821 tcgctgattg caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag
     2881 tattgaacat ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca
     2941 tcaatatcca caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact
     3001 gttaataaca agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta
     3061 tttgtgtata gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt
     3121 tgatggttcg cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt
     3181 ctgcgccatt cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc
     3241 cgttgcgttg tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt
     3301 aaattttctg cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa
     3361 agctgaatag cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg
     3421 cacctcattt ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt
     3481 gacaaaattt caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt
     3541 aaaaacagtg ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta
     3601 gtatctgtat cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc
     3661 tctaagaata gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa
     3721 actccttccg aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt
     3781 tttgctatat aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct
     3841 aatgctgcca tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata
     3901 gagcccttat aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc
     3961 attttcaaag tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt
     4021 gatacttgtt aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg
     4081 atatctttcg atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac
     4141 agagacattg tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc
     4201 tttttgatct ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt
     4261 gaatgtgagc aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa
     4321 tgctggaatt acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt
     4381 tttcgattac tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc
     4441 gctctggtct tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa
     4501 atagcagttc ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca
     4561 ctaagtagac acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc
     4621 ctgttttatt caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc
     4681 gtccgctctt tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt
     4741 ctagcagcaa aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat
     4801 acttcccaat ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta
     4861 ttccaataaa agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt
     4921 cgtttttcaa attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat
     4981 gcgagaactg gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac
     5041 aacgtatatc ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc
     5101 tcgtttgata tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg
     5161 aagcgcatag agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg
     5221 gtctacatga ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat
     5281 gatcagtaag acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct
     5341 ctttttcatg gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa
     5401 actaataaaa tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag
     5461 tgatgaagac gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta
     5521 cctaaacttc cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa
     5581 cgataattct ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta
     5641 gaggcaagat gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc
     5701 cttaattcga aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga
     5761 tacaaggaat atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca
     5821 ggagacaact tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg
     5881 aatagtagaa cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg
     5941 accattcctg aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct
     6001 agcaaaaagg tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat
     6061 ccttcaacaa gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga
     6121 cgttcagcta actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc
     6181 tcactaaacg cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga
     6241 aacgaacgga tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc
     6301 agttctccca tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag
     6361 ttctgactgc caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac
     6421 tgattcttcc tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc
     6481 caaaagttct tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt
     6541 cagttcatgg attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac
     6601 ccggtgccct gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc
     6661 gccaatgatt agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt
     6721 ctgtgctatt ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg
     6781 atcttcgata gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt
     6841 cttgcctgat ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa
     6901 aatagttgca aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa
     6961 tagccgaatc actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg
     7021 aacttcctgt ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc
     7081 gatatccatc tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga
     7141 gactttcttc cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga
     7201 agctggcaat atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc
     7261 cgataatcgt tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct
     7321 ttttgtttca aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat
     7381 acttataaaa atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg
     7441 aagtagcaag taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg
     7501 tctttacgaa agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa
     7561 tcggtgaata cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat
     7621 tcatcgatgg ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag
     7681 tatccttgct tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg
     7741 ctgggagtac aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct
     7801 tttttatgct accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa
     7861 atgcaaaaaa attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca
     7921 tgaactatca agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc
     7981 accagcataa gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg
     8041 tttatcctgc agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag
     8101 aacgtttgga tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg
     8161 catcgctact accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg
     8221 ataaactgat caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta
     8281 ttgcacttgg aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat
     8341 gttttgattt gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa
     8401 cagacgatct taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc
     8461 aatctgggca actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc
     8521 tgccaaaact aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt
     8581 cacaagccca tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa
     8641 aagtggaccg acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg
     8701 cggtgatcga gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc
     8761 agctgaaaca aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg
     8821 ttgggacaaa cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg
     8881 ggatgaccgt tgtcaacaat cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa
     8941 tcatgacaca ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag
     9001 cacatcaact aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat
     9061 tatctgtcat acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc
     9121 aattgattcc accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc
     9181 cagaaaatac attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt
     9241 ttttatgaaa gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg
     9301 agcttctact cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat
     9361 aatattgcct tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc
     9421 atattgagaa aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg
     9481 gatttctcca tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa
     9541 tcgccgtttt tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc
     9601 tacttcgctg tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag
     9661 gttaggaagc ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat
     9721 ttctactcca atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc
     9781 tttccccata ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt
     9841 tcccttccac aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc
     9901 tgctcccgga cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa
     9961 ggtctggcca aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa
    10021 ttgtaaaatt tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc
    10081 ttggagcaaa ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg
    10141 tatgccataa ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat
    10201 cgttgcaata tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa
    10261 ataattttta catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt
    10321 ttccttatct attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg
    10381 atttttctgg agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt
    10441 catgacacga ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg
    10501 gtacaaaaca tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc
    10561 ttccagccac tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag
    10621 ttttggtgta attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat
    10681 aaagactttg tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg
    10741 acgtaaccgc cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact
    10801 ctttgttttg cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac
    10861 gttctttttc atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa
    10921 cagcattctt actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt
    10981 tttcagtctg acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta
    11041 acaatcaaat aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca
    11101 atcaaaccaa aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc
    11161 tgcttcttct gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg
    11221 ttcgctaaag gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga
    11281 ctaaaataaa agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa
    11341 taaagaccac taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg
    11401 ttttttgatt ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc
    11461 tttgccgtaa tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac
    11521 ctatgttctt ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac
    11581 aaaaaccagt aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc
    11641 caataaaatg atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa
    11701 cgaataactt gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat
    11761 ttcaaactcc ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta
    11821 accaccacta atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg
    11881 caagaatagc gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa
    11941 aaaacaatat ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc
    12001 caaaatacag agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa
    12061 ttagaataat ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg
    12121 ttcaatacat aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga
    12181 aaaaaactac tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga
    12241 atccggcagc tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc
    12301 gttatgtaag accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga
    12361 catcaaaata ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat
    12421 agctagtgga tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa
    12481 aaaggcgcat aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca
    12541 gtcaagacga ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat
    12601 atccataggt atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc
    12661 ttcatataaa gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt
    12721 tttctttaag gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat
    12781 aatgtgcttg atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc
    12841 tccaggctgt acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt
    12901 gacatgatcg ataattaaac tcattctttt tcctccgcca tttattttta tttattatac
    12961 cacgaagaaa atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag
    13021 ttaagctgaa gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt
    13081 gcaaaatcat caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg
    13141 cttttttaga catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg
    13201 tagcagatat ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta
    13261 agattgctca agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata
    13321 acaaccgtga actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct
    13381 acagcaaaga ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg
    13441 aagaactaaa agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac
    13501 tttttcaaag gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg
    13561 cccaggagcg ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact
    13621 ctgacaaatg atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa
    13681 gaaacagctg aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat
    13741 tttaaattcc aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa
    13801 gctgatctgc cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa
    13861 ctatataagt gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt
    13921 ttgaaactac cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa
    13981 aataataact aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag
    14041 ctactagtgc gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata
    14101 tcgcaacgat gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg
    14161 aatcatcgaa ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat
    14221 atggcgataa agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat
    14281 taggtgatac ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag
    14341 aatatattcg taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag
    14401 atgacgattt gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca
    14461 aattatcaag tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt
    14521 tctctaaatt agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag
    14581 ttaaattcga ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat
    14641 tgaaagacgg tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag
    14701 aatactacgt tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca
    14761 aagatcaact aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta
    14821 aagtaatcgg tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa
    14881 atgtgttgag tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag
    14941 cttcaacaaa gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag
    15001 aaacaactga ttcatcaaaa taaa
//
LOCUS       DAYYCM010000067        14222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000067 DAYYCM010000000
VERSION     DAYYCM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.068"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAY1_002239"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAY1_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8594202.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAY1_002240"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAY1_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8594203.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAY1_002241"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAY1_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8594204.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAY1_002242"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAY1_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594205.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAY1_002243"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAY1_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8594206.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAY1_002244"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAY1_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8594207.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAY1_002245"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAY1_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594208.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAY1_002246"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAY1_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8594209.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHAY1_002247"
     CDS             complement(7139..7417)
                     /locus_tag="ACHAY1_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8594210.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHAY1_002248"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAY1_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8594211.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAY1_002249"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAY1_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8594212.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAY1_002250"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAY1_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8594213.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAY1_002251"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAY1_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8594214.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAY1_002252"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAY1_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8594215.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAY1_002253"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAY1_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8594216.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAY1_002254"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAY1_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8594217.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAY1_002255"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAY1_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594218.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14222
                     /locus_tag="ACHAY1_002256"
     CDS             13982..>14222
                     /locus_tag="ACHAY1_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594219.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGY"
BASE COUNT         3938 a         3057 c         2239 g         4988 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 tt
//
LOCUS       DAYYCM010000068        14129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000068 DAYYCM010000000
VERSION     DAYYCM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.069"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAY1_002257"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAY1_002257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8594220.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAY1_002258"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAY1_002258"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8594221.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHAY1_002259"
     CDS             8118..9086
                     /locus_tag="ACHAY1_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8594222.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHAY1_002260"
     CDS             9104..10009
                     /locus_tag="ACHAY1_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594223.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHAY1_002261"
     CDS             10006..10812
                     /locus_tag="ACHAY1_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594224.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHAY1_002262"
     CDS             10969..11937
                     /locus_tag="ACHAY1_002262"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8594225.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHAY1_002263"
     CDS             complement(12254..12778)
                     /locus_tag="ACHAY1_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8594226.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACHAY1_002264"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACHAY1_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8594227.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAYYCM010000069        13809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000069 DAYYCM010000000
VERSION     DAYYCM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.070"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHAY1_002265"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHAY1_002265"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8594228.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHAY1_002266"
     CDS             complement(2262..2513)
                     /locus_tag="ACHAY1_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8594229.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHAY1_002267"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHAY1_002267"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8594230.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHAY1_002268"
     CDS             3463..3681
                     /locus_tag="ACHAY1_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594231.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACHAY1_002269"
     CDS             complement(3970..4482)
                     /locus_tag="ACHAY1_002269"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8594232.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHAY1_002270"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHAY1_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8594233.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACHAY1_002271"
     CDS             complement(6935..7378)
                     /locus_tag="ACHAY1_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8594234.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACHAY1_002272"
     CDS             complement(7379..8167)
                     /locus_tag="ACHAY1_002272"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8594235.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHAY1_002273"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHAY1_002273"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8594236.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACHAY1_002274"
     CDS             complement(9282..9869)
                     /locus_tag="ACHAY1_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8594237.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHAY1_002275"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHAY1_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8594238.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACHAY1_002276"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACHAY1_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8594239.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="ACHAY1_002277"
     CDS             complement(12786..13721)
                     /locus_tag="ACHAY1_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8594240.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DAYYCM010000070        13399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000070 DAYYCM010000000
VERSION     DAYYCM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.071"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(348..1220)
                     /gene="scrK"
                     /locus_tag="ACHAY1_002278"
     CDS             complement(348..1220)
                     /gene="scrK"
                     /locus_tag="ACHAY1_002278"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8594241.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1484..3430)
                     /locus_tag="ACHAY1_002279"
     CDS             complement(1484..3430)
                     /locus_tag="ACHAY1_002279"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8594242.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            3615..5054
                     /locus_tag="ACHAY1_002280"
     CDS             3615..5054
                     /locus_tag="ACHAY1_002280"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8594243.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            5056..6018
                     /locus_tag="ACHAY1_002281"
     CDS             5056..6018
                     /locus_tag="ACHAY1_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8594244.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(6188..7614)
                     /locus_tag="ACHAY1_002282"
                     /pseudo
     CDS             complement(6188..7614)
                     /locus_tag="ACHAY1_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7857..8312
                     /gene="tnpA"
                     /locus_tag="ACHAY1_002283"
     CDS             7857..8312
                     /gene="tnpA"
                     /locus_tag="ACHAY1_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8594245.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            8577..8675
                     /locus_tag="ACHAY1_002284"
                     /pseudo
     CDS             8577..8675
                     /locus_tag="ACHAY1_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8898..9128)
                     /locus_tag="ACHAY1_002285"
     CDS             complement(8898..9128)
                     /locus_tag="ACHAY1_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8594246.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(9358..10176)
                     /locus_tag="ACHAY1_002286"
     CDS             complement(9358..10176)
                     /locus_tag="ACHAY1_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8594247.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            10337..11026
                     /locus_tag="ACHAY1_002287"
     CDS             10337..11026
                     /locus_tag="ACHAY1_002287"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8594248.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            11040..12542
                     /locus_tag="ACHAY1_002288"
     CDS             11040..12542
                     /locus_tag="ACHAY1_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8594249.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            12555..13031
                     /locus_tag="ACHAY1_002289"
     CDS             12555..13031
                     /locus_tag="ACHAY1_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8594250.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(13128..13238)
                     /locus_tag="ACHAY1_002290"
                     /pseudo
     CDS             complement(13128..13238)
                     /locus_tag="ACHAY1_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4390 a         2242 c         2360 g         4407 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga acctatttgt taaaaaggtc
      121 caaaaaaata tccgcacccc cttttttttg taaaaaacta ttgcacaata caagtggcct
      181 acggctatcg aaatttccat aactttcgag caagaattta tctcattcaa ggcttgatct
      241 tccaagtaaa acaaaaacca gttaagcact ctgcctaact ggttatgact aaattttcag
      301 ttgtatcttt ctctaccaac actaaatgtc gaagagcctt ttttcgttta tgaatgtaag
      361 accgcttctc gagccaatgc caaacaaccg agcgttccag cgttgtcttc taatgctggg
      421 gtcacgatat atttgcttaa ctcaggtgtt tcgacatacc cattgaccaa ttttgcaaat
      481 gcgtcgtgga cgttttgcag catatggcgt tgtttcatca caccaccgcc aaaaatgatc
      541 acatctggag aaaacatcaa ggttgtattg taggcacatt gagcaatata ggaggcttcg
      601 atttcccaat aaggatcatc ttctgcaacc tcttgtcctt ttttccctaa gcggttttcg
      661 atggcgggtc cagcggccat cccttctaaa cagttgccat gaaatggaca gctgccagca
      721 aagtcatctt ttggatgagg gacgacgagc atatgcccca tttctggatg actaaaccct
      781 tcgataaagc gcccttcttg taaggcaccg ccgccaacac ccgtaccgat cgtatagtaa
      841 acgacactgc tcaatccttt gccactgccg aagacatact caccataggc agccgcattg
      901 acatctgttg tccaagaaat cgggatcgga aattcttttt tcatggtacc gatgaaatca
      961 aaattttgcc atgccaattt cggggtcgag gtaatgtacc cataagtggc ggaatcgcga
     1021 tggatatcga taggcccaaa tgagccaatc ccaatccctg ctaaatctgc ttggtattgt
     1081 ttgaagaagt caatgactaa aggcattgtt tcctcaggtg tcgttgtcgg aaagctcact
     1141 ctttccttga tcgtcatttc ttcatcgcca atggcacaca caaattttgt gccgcctgct
     1201 tcaattgcac cgtatagcat agacatttct ccctttattt tagaatgaaa tcactaaata
     1261 aatgactata tatcatattt aaattatttt tttatttagt aacatgacaa gtaaataact
     1321 actatttttt ctttttgaat ttttatcatt ttttaaaatt ctaataattt ttcatacaat
     1381 tgatatatga aaagattaaa taaaatgtta ttctcaaata tttgattaat ctatattatt
     1441 tgattttgga aagaaaattc catctttact cagggaaata tatttatttg atttcaagta
     1501 aatcagtacc ctcagtgact gtattttcag ctactatatt tacttctgaa taatccgaag
     1561 tattggtcac aataatcatt gttgtatcat caagaccttc tttctcaatc attgaacggt
     1621 caaatgctcc aagaatatcg ccttttctaa ctttttgatt agtagtaact ttttgtgtaa
     1681 atccttttcc tcccattgat acagtatcaa taccaatatg aattagaact tcagcaccat
     1741 tatttgattt aataccataa gcatgtccag tttcaaacgt gagttgaata gtaccatcta
     1801 ctggagaatg gaccgtgttt ccatttggtt taatggcaat cccttttccc atagcttctg
     1861 atgaaaagac tggatcattt actgaagaaa gactaatcac tttaccagaa atgggactaa
     1921 caattgtttc tgatgaaact tttgtttgtg ttgattcaac atcagtagat attggatctg
     1981 ttgttgctac ttttgaagaa ttaactggtg tttgttcagc aatttcttct gctttaaaga
     2041 taccagcagc ttttgtttta ccgtagaagt aagtaattgc aaaagcaact acaaagctaa
     2101 tcaattcaca caatacgtaa aatggaatag atgttgcatt aattgaaaga aatcccaaga
     2161 aacctgctga accaagtgaa acagcaacta ctttaagtaa tcctgctact gctgtagcaa
     2221 ttcctgaacc aattaaagca caaaagaatg gaaagcggta ttttaagtta actccgaaca
     2281 aggcaggctc tgtaattcca agaagtgctg agacacccgc agaggaagca agactttttg
     2341 tcttagaatt tttagttaag aaccaaatgg caaatgttgc agcaccttga gcaacgtttg
     2401 ccatagatgc aactacaaag ataaagtctc catgtccgat accatttgtc cacgctgaaa
     2461 gtaattgagt ttcaatagct ggaaatgatt gatgtaaacc agtcatgact ataagtgaat
     2521 atgttcctcc aaaaataccc attccaataa atccagtagt gttatatagc caaacaataa
     2581 tgtctgtaat tccattagac aacaataaca ttacagggcc aataactgtg aaagtcaaaa
     2641 atccagtaat cattactgat aataatggcg tgaatgtaaa gtcaatagat gatggcaatt
     2701 ttttatgaaa gaatttttct aatatagata atatccaaac agctgctaaa actggaataa
     2761 cttgataagc atagtttgtt tgtgaaacat gataaccaaa aatattccaa tattcagttg
     2821 agccaccaag atctggtgtt gtcatgatca taccaattgc agcaccaaga aattggtttg
     2881 ctccaaaacg tttagcagct gaaataccta ctaaaattgg taagaacatg aatggtgcag
     2941 cggacatcag ttggatcatt gaggaaatac cttcaatcgc aggatacatt tcttcaattg
     3001 attttggtcc aaaaagtcca gcagctgtta aaaagttttg taatgccatt aataaaccac
     3061 ctgcaacaag ggcaggtata attggaacaa aaatatctga taataattta attaatgcca
     3121 taatggggtt gaatttttta tctttactag caatttgttt taaatcatct gttgaaattt
     3181 ccgaaagtcc agtttttttg ataagttcgt cataaacgaa attaacatca cctgcaccga
     3241 taatcacttg atattgtcca tcaattttaa atgtgccttt cacatctgga ttttcgtcta
     3301 aagctttttg attcactttc gtgtcatctt ttagtacgag acgaagacgt gtagcgcagt
     3361 gtgcagctgc aataaggtta tcttttccga ccgcttgtat tacgtcggaa gctactttac
     3421 tgtaattcat tcgaatcact ccttttttat ttttacgtga aaacggttca ctgaaatgca
     3481 aatttatttt agcatatctt aaaaatatgt caaacgtttg tcatatagtt tattttttta
     3541 aatgaaatga ttgatttttc aacggaaaac gtttaccata ctagttgtaa aaagataaat
     3601 tgaaggagta aaatatgaac ttacctcgag aaatacgata tcgtgcttat tctgattgga
     3661 ctaaagatga gatggataaa attaaggata atgtaaaacg atcaccttgg cgtgctagct
     3721 atcatatcga acctaaaaca ggattgttaa acgatcctaa tggtttttct ttttttaacg
     3781 gaaaatatac gcttttttat caaaattggc catttggagc tgctcatggg ttgaaagaat
     3841 gggtacacaa tgaatcagat gatttagttc attttcatgt aacaggagca gaacttcttc
     3901 cagacacaaa acgagatagc cacggcgctt attccggttc tgcatatgaa atagagggga
     3961 atctattctt actctataca ggaaacgttc gaaatgaaaa ttgggaacgt gatacacttc
     4021 aaatcggagc atggatggat aaagactaca acattacgaa aggtgagaat gttcttattc
     4081 ataaacctgc tgatgtaact gatcattttc gtgatccaca aatgtttaat tatgaaggac
     4141 aattgtatgc gattgttgga gcacaaaatt tagaaaaatc aggctttatc aaactttaca
     4201 aagcggatga caataacgtg gaaacatggg aggaagttgg caaccttgat tttggcggca
     4261 ctggttcaga atacatgatt gagtgtccaa atctagtatt tgtagacaaa aaacctgttc
     4321 tcatttattg tcctcaaggg ttggataaat ctgagctaaa ttatgggaat atctatccga
     4381 atacttttaa gatctgtcaa tcgcttgaca tagaaaaagt aaaacttgtg ggcgcttcag
     4441 aaattcaaaa tcttgattat ggatttgaag cgtatgcgac tcaaggattc aatactcctg
     4501 atggtcgtgc tctgattgtc agttggattg gactacctga tatcgattat ccaactgata
     4561 agtatgatta tcagggagca atgagtcttg taaaagaact ttcaataaaa gatggtaaac
     4621 tttaccaata tccagtagaa gctattacat cattacgtgc tagttctgaa caatttacag
     4681 caaaagcaga aacaaataat acttatgagc ttgaactaac ttttcctcca aatcagaaat
     4741 cggagttact tcttttctct gatagtaaag gaaatggact tagcttgatt gtagatacca
     4801 aagaaggaaa aattatttta gatcgcagca aagcaggcat tcaatatgcc actgactttg
     4861 gtacgattcg tgaatgctca attgatacta aagaaacaac tgctaacatt tttgtagata
     4921 actcaatcat tgagattttt attaataaag gagaaaaagt gtttactagt cgtgtattcc
     4981 ctgaaaatca ccaaaatggt attcaaatca aatcaggcga acctactgga aaatactttg
     5041 aattaaaata ttaaaatggt agcaaaatta actgatgtcg ctgaattagc tggagttagc
     5101 ccaacaaccg tttcacgcgt cattaataat aagggatatt tatcagaaaa aacaaaacaa
     5161 aaagttcatg aagctatgaa aactcttggt tataaaccaa ataatttagc acgtggactt
     5221 caaggaaagt cacctcagct tattggtctt atttttccta atattagtaa tatcttttac
     5281 tctgaactca ttgagtatct cgaaatagag ttatttaaac atggatataa aacgattatt
     5341 tgcaatagtc aaaatgatcc tgctaaagag agagaataca ttgaaatgct tgaagccaac
     5401 caagttgatg gcataatctc atcaagccat aatcttggaa ttgctgatta tgagcgtgtg
     5461 agagcaccta ttgttgcttt tgatagaaat ttagctccta atgtttccat tgtttcttca
     5521 gataactttg aagggggaaa acttgcagct aagactttac aaaaaaatgg ttgtcaaaat
     5581 accattatga ttacaggaaa tgataacacg gattctccca cagaattacg tgcactaggt
     5641 ttttcttttc aaaatccaaa tggaaaaatt ttcaaaattc ctaataatct gtctactatc
     5701 cgtcgtgaaa tggaaataaa gtcaactatt ataaatcaca aaccagatgg tatttttgtt
     5761 tctgatgatt taacagccat tctcactatg aaaattgctc atcaacttaa acttaatatc
     5821 ccagaagatt taaaaattat tggatacgat ggtacctctt ttattgagaa atttttccca
     5881 caattgacaa caataagaca accaattgat gaaattgcaa gtcttattgt tgatattctt
     5941 ctgaaaaaaa ttaaaggcga aaagacaaat aaggattata ttttaccaat ctcaatactt
     6001 tctggaggaa gtatttaacg ttaaattaat tttttaatgt ctataaaatt cgttagaaaa
     6061 tacaatttgt agatatccaa ttgatttgta aacaaaaagg agttgtcaac ttgtagtgac
     6121 ccccaaaagt tagacttttt tggagtggag aaatccactc cttttttcat gctctaaaat
     6181 gatattttta cacaactgtt acggttgttt tacgaaattg gacgggactt tgccagttta
     6241 atttctgttt tatccgttca ttattgtagt aatagatata gctatcgatg gctgatttta
     6301 attcctcgaa acagttataa actttcccgt gaaaaatctc ttgttttaat aagccaaaaa
     6361 aattctccat aagtgaatta tctaagcagt tccctttccg agacatgctt tgaaagattt
     6421 tgtactcctt caatttgttt ctataagctt tcatttgata cgcccaacct tgatctgagt
     6481 gaaacgtacg acgataggga caatcgttag tggtctggat agcttcctcc aaaccttcca
     6541 ttaccgctaa tgcattgggc ttttcagaga tacgatagga taaaatctca ctattgaaca
     6601 tatccataaa aggatctaga tacaatttct tttgacggat aatgcctttg gcatccactt
     6661 catggtattt aaattcagtt gtatccgtcg tgattttctg atggcagata ctggtgtaga
     6721 aacgacgatg gatccgattt ttggctactg ctccgacttg tcctcgataa gaattgtagc
     6781 gccgtgattt acgtgtgtat gtcgttactt ttattcctag cttcttgatc aggcgctgaa
     6841 ctttcttttt attgatagag aaaccgcggt ttcttagttc attggtcatc cgtaaacagc
     6901 cataatcctt gtgcttctcc cgaactttaa gcatttccgc ttcaatctct tgatctggat
     6961 ttttacggtc caatcgtttt tgccagtaca taaagattgc cttagggaac tttaaagttt
     7021 ctagaagctc aactaattta aacggtcctc ggaggcttgc gatgattcgt gcgattgctt
     7081 cattcgtcgt tgttgggctt cctgttttcg cagcttcctc aattctttta aaaaggcatt
     7141 ttcaatttga agggaacgga ctcgtttctc caactcctcg atgcgttcac gttctttaga
     7201 cgttgcttta gttgtagatt ttttcttatt tttgggttca tttttcttag acaagatagg
     7261 tggacatcct ttcgtttttg agagtccatc gatcccaccc tcacggaatt gacgcatcca
     7321 actagcgatc aaattagggt tgttcatgtt aagtgtgttg gcaacttcac gataggacag
     7381 ctcacttgtt agatataact ctatcgcatc cagcttaaat tgaacagaat attttttctt
     7441 ttgtcgcttt cttactagac cctcttcacc aaattcttga taggcattga tccagcgctt
     7501 cacttgtgat ggagccttaa tggaatattt ctttgccaga taatcacttc cgcctttacc
     7561 atccaagtaa tcatgaacga tctttaattt aagttcaaaa gtatacttag ccataaaaaa
     7621 taccgacctc caaaagttag attttttggt ctaacttttg ggggtcggta caaaacttaa
     7681 aagttttaga cccttttatt ttcttaatga cttcagtcag ttttgtgcgt aaagcgtgcg
     7741 aaacaagaaa accgcgcgtt tctacgcgtg gaagaagtat ctttagcgat aaaaactcct
     7801 ttcgatatac tagtgagtgg ctaccaaccg ctcatcagat cgaaaggagt tttttcatgt
     7861 caaatgacga taaaagttta gcatatactc gttggaattg taagtatcat attattttta
     7921 caccaaaata tcaacgaaaa gtaatctatg ggcaattacg aaaagatctt ggaaaaatac
     7981 ttaggaagtt gtgcgaaatg aaagatgtgg aaatcataga agcacatgca atgctagatc
     8041 atattcacat gcttgttcga gtaccaccaa aaatatgtat atccagtttt atggggtatc
     8101 tgaaaggtcg tagtgcggta ctaatccacg agcagcacgc aaatctcaaa tataaatacg
     8161 gaaacaaatc cttttggtcg aagagatact atgtgagtac agtcggattg aatcaaaaga
     8221 caaatgaaaa atacatccgt gaacaagaag ctgatgatcg cattcgatat attataagta
     8281 aaagagagta cgtagaacca tttaaaaaat aaagagaagt aagtgagtga agatgagcag
     8341 ttggcggtct tttaaaaatc cccttttaag gggcagcgag tgtcgacccc ttctaggggg
     8401 attaaaaata cacccgtcct cacgggtgta tgggttactg atagacttga ttttaactgg
     8461 tgtcagaaac ttatgtccag aaagggtgat gaaattttgg caatttacgg ctattcaaga
     8521 attagcacaa gcaaccaaga ttacaaaaca caaattcaaa cattagaaaa tgctggcgca
     8581 gaaataatct tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
     8641 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagagtatat ttacttcaga
     8701 tggtaaggca acaaaataaa atacaatagg cagatgaact aataaataaa atctgtatta
     8761 aatacagata aattaatata aaaatcaaaa ggatcataaa cttttaacaa gtgatgaaat
     8821 gggtggaaat tgattcttat agacagtttg tctcataaca ttgaagtatt ttgcaatttt
     8881 agcattgaaa cactttttta atcttcaacg ttgttcttat aaacaagatg attgggaatc
     8941 ttttgaatta acactgtata gcttagaaca ccctttatag aaaacattcg cttagcaatc
     9001 ttaataattc cttgtgcccg atgtcgttta gattcagtag gttcctgctc atcgatcatg
     9061 gaaaaaattt gaaggatcag atccttaata aatttattta agagaggatg atccattgct
     9121 tcatccatac tcgataaact agtgataatg agctacacgt ttttctttca agttgatcaa
     9181 tagcttccac gataaaaata tctttagata aaatcaagaa ccgtaggaac tacagggata
     9241 tttggtcaat aagagccacc gctattagta aaaaataagc tatcaagtat gctctcatcc
     9301 caagaattgc ctacttcaat cgttaaaaac cgttaggttt agctaagtgg gggtagttca
     9361 ctcaatactt ttatcaataa tagagcgcaa aactttttct cttaattcaa ctgaatcaat
     9421 attattgttt tgttcataac catgaactaa tagatcacaa atataaagtt gtgatatttt
     9481 tccagctaaa gatccaccat taagaaattc ttcaatggct gtttgtaaaa ctaagtctgc
     9541 agactttcct ataggtgaat gaatatagtt agttatagca ataatttttg cgccattttt
     9601 tttagctatt ttcaatgaat cataagtatc tttcgttttc ccagataaag aaaaaatgat
     9661 tactaaatca ttaactgtta ataaagaagc gacttgggct tgaaaatgag ggtctagtac
     9721 agcttttgct tgaactccta cgcgaagaaa catattttct aaatcaagac tagtatttcc
     9781 actagatccc actccaaata tatatatgtt ttttgattga gtgatcattt taatggcttc
     9841 attcaatttt tcttcgtcta aaagcctaat agtggaatag agagcttctg ttaaactatt
     9901 agcaatttca tcatagtact ttacactagt cggatgttct tttttttgac taaagtcttc
     9961 ctttgcaatt tcaattttta aatcagaaaa accagaaaaa ccaatttttt gacaaaagcg
    10021 aataatagta gcatcgccaa cttttgtgtt tttcttaata tcactcatcg tactataaat
    10081 aatagttttc ccagaattta aaataaaatc agcgactttt ttttcagact tcgttaaaaa
    10141 tggataattt tctttaatta aatagatata actcatacaa actcctctac aatttcaact
    10201 tttctccatt atactccaaa ataataaaaa aaactcctaa atactccatt tttatcttta
    10261 cttttatgaa agcggatgct atattgtttt tggagttgtt atccaaaaat aataaaaaat
    10321 tggagtgttt aggataatgg ataaaaagag gtttttagat cttatttcaa aaggtttgat
    10381 tgtttcctgt caagcattac ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc
    10441 ttattttgct ttggctgctc aaagggctgg agcagttggt atacgtgcaa attctgttcg
    10501 agatattatt gaaattagaa agtgtgtatc attaccaatg attggaatta ttaaaagaga
    10561 ttatccacca gaatctccat tcattactgc cactatgaga gaagtagatg aattagtaga
    10621 aaccggtgtt gaggtaatcg ctcttgattg cactcttaga aatcgacatg atggactatc
    10681 tataaattct tttattacta aaataaaaga aaaatatcct catcagttat ttatggcaga
    10741 tatttctaca tttgaagaag gcaaaaatgc atataaagca ggaatagatt ttgtaggaac
    10801 tactttaagt ggatatactg aggaaagtaa aaaacaagat gggccagata tagatttgat
    10861 taaaaaattg gtagaagaag ggatttgtgt aattgctgag ggaaggattc atactccaga
    10921 acaagcaaaa gagataaaaa aaataggagt tgcaggtatt gttgtgggtg gtgctattac
    10981 tcgaccacag gaaattgcat caagatttgt taaagcatta agttaggggg agaaataaaa
    11041 tgtttaaaaa attttcacaa ttaggacgcg ctttcatgtt accaattgct atattaccag
    11101 tagccggatt actattggga ctgggaggag cgttaacaaa tgagagtgct ataaatgctt
    11161 atccattttt agatcaaccg tggttgcata caattttaag tattatgagt tatgctggaa
    11221 atgcagtttt tgcaaaccta gcactaattt ttgcaattgg tattgctgta ggacttgcaa
    11281 atggagataa aggaacagct ggtttagctg gtggagtagc ttatctagta tatactgcaa
    11341 ctattagtgg gtttcttgaa cttttttcag caaaagatgc aacactagat acaggtgtag
    11401 tgggatcaat tgttgttggt ggtactgttg catttttaca caatcgttat agaaaaattg
    11461 aattaccaca attcttaggt ttttttggag gatcaagatt tattccgatt gtttctagtt
    11521 tagctgcaat cattattggt tctttctttt atttaatttg gccaccaatt caaaatggtt
    11581 tagtagttgc tggtgaacaa attgcccaaa tgggtagttt aggaactttt ttatatggtt
    11641 ttttattgcg tttaacaggt gctgttggac tacaccacac catttaccct ttgttctggt
    11701 atacatcttt aggtggtacc gaaactgtag ctggatcaac aattgctgga gcccaaaata
    11761 ttttctttgc ccaactagct gatccaaacc acactggtct gtttacttat ggtactagat
    11821 tttttgcagg aagatttgca acaatgatgt ttggattacc agcagcatgt tatgcaatgt
    11881 atcgtgcaat tcctaagaaa aatcgtaaaa agaatggtgg gctttatttt agcggggccc
    11941 ttacatcatt tttaactgga attactgaac cagttgaata tatgttttta tttgttgctc
    12001 cttggcttta tgtgattcat gcattcttag atggattatc tttttatttt gctgatatct
    12061 taaatattcg tgtagggaat tccttttcag gtggtttgat cgacttttta ttattcggtg
    12121 tacttcaagg gaatgacaaa actaattggt taaaattaat tccttttggt attgcatggg
    12181 catttatata tttctttgtt tttacattct ttatcaaaaa atttaaggta gctattcctg
    12241 gaatggaaga agaatcagaa gttcattctg aaaaagtaat aaacaaaaat acatcattac
    12301 atgaagagtc tcttatcatt attgaagctc ttggtggaaa agaaaatatt gagtctgttg
    12361 aagcttgtgc aactcgtttg cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa
    12421 caataaaaga cactggtgca actgctgtct tcgaagtgaa gggtggcatc caagctgttt
    12481 ttggagggaa agctgactta ttaagtcaag aaatcaatca aatattagga aaagataatt
    12541 aggaggtttt taatatgttt gaaattttca aaaagaaaaa acaagaaact ttttatgcac
    12601 cttgttccgg aaaaatcatt tccatcacag aagtaaatga tcctgtcttt tcacaaaaat
    12661 tactaggtga tggttttgcc gtcatacctg atggttcaaa aatttatgct ccattggctg
    12721 gtcgagtaac aaatatattt cctactaaac atgctattaa tataaaaagc aactcaggca
    12781 ttgattattt gattcatatt ggaattgata cagttgaatt acaaggagag cctttttcga
    12841 tttgtgttca agaaaatgat gaggttactc ctgaaacctc gttggttgat gtggattttg
    12901 acaaaataat aaaatctggt aaagatactt ctgttatagt tgtttttccg gagaaaaatc
    12961 agttcaatga tttaataata gataatggtc gaattgctca tgggaattca tgtggaaaaa
    13021 tcacttttta aaaaagtgta tataaaaagc tataaaataa gctaaatagg atatttgtca
    13081 aatgaagttg gtgaacagaa agaactgttt tctttctgtt cattttttta ttttattagt
    13141 aatgattccc catgccaaat catgatccgt aatttaaagt tataaaagct tcgaaatcca
    13201 ttggcatttc gttggaatac cttgatatga ttattcaaat ataaaatata gagagaagag
    13261 agagagggaa gatatttata aagggcaaag ttatgtttac tcaacttagg aaatagaaat
    13321 agatcaattt atattctctt gtcaatcgaa tatgaacctt agcactatat accaaatata
    13381 gtaaaatgct gaaagatgt
//
LOCUS       DAYYCM010000071        13293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000071 DAYYCM010000000
VERSION     DAYYCM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.072"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(439..1053)
                     /locus_tag="ACHAY1_002291"
     CDS             complement(439..1053)
                     /locus_tag="ACHAY1_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8594251.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1659..2036)
                     /locus_tag="ACHAY1_002292"
     CDS             complement(1659..2036)
                     /locus_tag="ACHAY1_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594252.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHAY1_002293"
     CDS             complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHAY1_002293"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8594253.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3260..4279)
                     /locus_tag="ACHAY1_002294"
     CDS             complement(3260..4279)
                     /locus_tag="ACHAY1_002294"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8594254.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4912..5916)
                     /locus_tag="ACHAY1_002295"
     CDS             complement(4912..5916)
                     /locus_tag="ACHAY1_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8594255.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5929..6978)
                     /locus_tag="ACHAY1_002296"
     CDS             complement(5929..6978)
                     /locus_tag="ACHAY1_002296"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8594256.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6995..7816)
                     /locus_tag="ACHAY1_002297"
     CDS             complement(6995..7816)
                     /locus_tag="ACHAY1_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8594257.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7803..8582)
                     /locus_tag="ACHAY1_002298"
     CDS             complement(7803..8582)
                     /locus_tag="ACHAY1_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8594258.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8599..9069)
                     /locus_tag="ACHAY1_002299"
     CDS             complement(8599..9069)
                     /locus_tag="ACHAY1_002299"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8594259.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9081..9500)
                     /locus_tag="ACHAY1_002300"
     CDS             complement(9081..9500)
                     /locus_tag="ACHAY1_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8594260.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9581..12358)
                     /locus_tag="ACHAY1_002301"
     CDS             complement(9581..12358)
                     /locus_tag="ACHAY1_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8594261.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12549..12773
                     /locus_tag="ACHAY1_002302"
     CDS             12549..12773
                     /locus_tag="ACHAY1_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594262.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3913 a         2762 c         2157 g         4461 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1501 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1561 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1621 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1681 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1741 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1801 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1861 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1921 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1981 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2041 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2101 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2161 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2221 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2281 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2341 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2401 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2461 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2521 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2581 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2641 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2701 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2761 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2821 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2881 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2941 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3001 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3061 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3121 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3181 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     3241 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     3301 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     3361 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     3421 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     3481 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     3541 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     3601 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     3661 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     3721 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     3781 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     3841 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     3901 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     3961 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     4021 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     4081 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     4141 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     4201 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     4261 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     4321 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     4381 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     4441 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     4501 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     4561 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     4621 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     4681 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     4741 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     4801 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     4861 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     4921 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     4981 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     5041 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     5101 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     5161 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     5221 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     5281 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     5341 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     5401 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     5461 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     5521 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     5581 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     5641 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     5701 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     5761 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     5821 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     5881 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     5941 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     6001 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     6061 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     6121 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     6181 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     6241 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     6301 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     6361 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     6421 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     6481 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     6541 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     6601 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     6661 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     6721 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     6781 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     6841 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     6901 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     6961 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     7021 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     7081 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     7141 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     7201 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     7261 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     7321 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     7381 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     7441 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     7501 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     7561 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     7621 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     7681 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     7741 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     7801 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     7861 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     7921 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     7981 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     8041 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     8101 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     8161 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     8221 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     8281 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     8341 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     8401 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     8461 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     8521 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     8581 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     8641 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     8701 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     8761 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     8821 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     8881 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     8941 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     9001 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     9061 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     9121 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     9181 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     9241 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     9301 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     9361 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     9421 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     9481 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     9541 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     9601 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     9661 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     9721 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     9781 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     9841 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     9901 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     9961 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
    10021 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
    10081 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
    10141 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
    10201 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
    10261 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
    10321 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
    10381 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
    10441 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
    10501 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
    10561 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
    10621 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
    10681 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
    10741 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
    10801 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
    10861 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
    10921 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
    10981 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
    11041 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
    11101 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
    11161 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
    11221 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
    11281 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
    11341 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
    11401 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
    11461 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
    11521 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    11581 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    11641 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    11701 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    11761 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    11821 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    11881 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    11941 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    12001 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    12061 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    12121 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    12181 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    12241 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    12301 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    12361 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa
    12421 aatatatctt attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg
    12481 tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg
    12541 actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc
    12601 gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta
    12661 tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt
    12721 tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac
    12781 agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac
    12841 aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact
    12901 gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc cattgattac
    12961 gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa
    13021 aatctcaagt ggcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata
    13081 gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac
    13141 aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt
    13201 ctctcaaccc acttttttac aaaaattacg tagcaaaaaa gattgcaaga acaaattaaa
    13261 aaagaagtaa atagagtttc atctataaac att
//
LOCUS       DAYYCM010000072        13085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000072 DAYYCM010000000
VERSION     DAYYCM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.074"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHAY1_002303"
     CDS             complement(79..2199)
                     /locus_tag="ACHAY1_002303"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8594263.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHAY1_002304"
     CDS             2429..3607
                     /locus_tag="ACHAY1_002304"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8594264.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHAY1_002305"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHAY1_002305"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8594265.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHAY1_002306"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHAY1_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8594266.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHAY1_002307"
     CDS             complement(4960..6213)
                     /locus_tag="ACHAY1_002307"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8594267.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHAY1_002308"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHAY1_002308"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8594268.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHAY1_002309"
     CDS             7790..9136
                     /locus_tag="ACHAY1_002309"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8594269.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHAY1_002310"
     CDS             complement(9444..9830)
                     /locus_tag="ACHAY1_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8594270.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHAY1_002311"
     CDS             complement(10098..10247)
                     /locus_tag="ACHAY1_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594271.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHAY1_002312"
     CDS             10709..10921
                     /locus_tag="ACHAY1_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594272.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHAY1_002313"
     CDS             complement(11199..12998)
                     /locus_tag="ACHAY1_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8594273.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DAYYCM010000073        12936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000073 DAYYCM010000000
VERSION     DAYYCM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.075"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            276..833
                     /locus_tag="ACHAY1_002314"
                     /pseudo
     CDS             276..833
                     /locus_tag="ACHAY1_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            891..2930
                     /locus_tag="ACHAY1_002315"
     CDS             891..2930
                     /locus_tag="ACHAY1_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8594274.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3039..4190)
                     /locus_tag="ACHAY1_002316"
     CDS             complement(3039..4190)
                     /locus_tag="ACHAY1_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8594275.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4344..4874)
                     /locus_tag="ACHAY1_002317"
     CDS             complement(4344..4874)
                     /locus_tag="ACHAY1_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8594276.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4973..5323)
                     /locus_tag="ACHAY1_002318"
     CDS             complement(4973..5323)
                     /locus_tag="ACHAY1_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8594277.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5480..7009)
                     /gene="ahpF"
                     /locus_tag="ACHAY1_002319"
     CDS             complement(5480..7009)
                     /gene="ahpF"
                     /locus_tag="ACHAY1_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8594278.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7019..7582)
                     /gene="ahpC"
                     /locus_tag="ACHAY1_002320"
     CDS             complement(7019..7582)
                     /gene="ahpC"
                     /locus_tag="ACHAY1_002320"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8594279.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7726..8388)
                     /locus_tag="ACHAY1_002321"
     CDS             complement(7726..8388)
                     /locus_tag="ACHAY1_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8594280.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8572..10218
                     /locus_tag="ACHAY1_002322"
     CDS             8572..10218
                     /locus_tag="ACHAY1_002322"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8594281.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10209..11291
                     /gene="uxuA"
                     /locus_tag="ACHAY1_002323"
     CDS             10209..11291
                     /gene="uxuA"
                     /locus_tag="ACHAY1_002323"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8594282.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11399..12934)
                     /locus_tag="ACHAY1_002324"
                     /pseudo
     CDS             complement(11399..12934)
                     /locus_tag="ACHAY1_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4323 a         2369 c         2411 g         3833 t
ORIGIN      
        1 ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc
       61 tttttccaat agctaagaat gttttcttta cgttgtatag tgctaaaact cggacgatga
      121 gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg accttttctt tatatttttg
      181 tgactgactt atttgatgag acaaactcgc tcctcacctg acaagtggat tgctgtgtga
      241 ggaggagatg cggtgaaaaa agatcgattg gtggaggtcg ctcggattca cttctggttg
      301 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
      361 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
      421 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
      481 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
      541 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
      601 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
      661 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
      721 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
      781 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
      841 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
      901 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
      961 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
     1021 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
     1081 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
     1141 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
     1201 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
     1261 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
     1321 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
     1381 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
     1441 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
     1501 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
     1561 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
     1621 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
     1681 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
     1741 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
     1801 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
     1861 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
     1921 aattgacaat cgatgcgaag gcacaaaaaa tagcttttga cagtctagga ggaaaagctg
     1981 catcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
     2041 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
     2101 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
     2161 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
     2221 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatctttt aaaacaacgc
     2281 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
     2341 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
     2401 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
     2461 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
     2521 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttatcaac aatggcacac
     2581 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
     2641 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
     2701 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
     2761 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
     2821 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
     2881 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
     2941 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
     3001 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
     3061 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
     3121 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
     3181 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
     3241 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
     3301 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
     3361 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
     3421 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
     3481 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
     3541 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
     3601 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
     3661 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
     3721 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
     3781 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
     3841 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
     3901 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
     3961 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
     4021 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
     4081 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
     4141 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
     4201 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
     4261 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg
     4321 tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg
     4381 ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc
     4441 aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta
     4501 taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg
     4561 atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca
     4621 tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa
     4681 cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga
     4741 taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca
     4801 atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat attttgttta
     4861 atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt
     4921 accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc
     4981 tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac
     5041 gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg
     5101 taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca
     5161 cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga
     5221 tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat
     5281 aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt
     5341 tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
     5401 aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac
     5461 tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag
     5521 tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag
     5581 ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg
     5641 gattcatttc gatcgttcta tctagccaat ccgtattagg gagtaagccg actagaatga
     5701 atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag
     5761 attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat
     5821 tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt
     5881 ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat
     5941 taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc
     6001 aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt
     6061 gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa
     6121 tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga
     6181 tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag
     6241 aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag
     6301 cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg
     6361 gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca
     6421 aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt
     6481 ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga
     6541 acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca
     6601 atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca
     6661 atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc
     6721 cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag
     6781 caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg
     6841 aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag
     6901 ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa
     6961 tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct
     7021 aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg
     7081 ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca
     7141 gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt
     7201 ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa
     7261 aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag
     7321 catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga
     7381 cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag
     7441 gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc cccataagat
     7501 ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga
     7561 tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat ttataattat
     7621 tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca
     7681 aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata
     7741 ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc
     7801 cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg
     7861 ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg
     7921 ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc
     7981 gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt
     8041 gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa
     8101 tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt
     8161 ttctgcagaa acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac
     8221 gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag
     8281 atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat
     8341 ttgtttacga atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc
     8401 aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca
     8461 cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc
     8521 ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat
     8581 ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat
     8641 tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt
     8701 ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc
     8761 gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct
     8821 taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc
     8881 aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac
     8941 tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt
     9001 actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc
     9061 gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt
     9121 tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca
     9181 caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa
     9241 ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca
     9301 tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca
     9361 aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca
     9421 tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc caatggacgt
     9481 ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg caacggtaga taaagcagat
     9541 acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt
     9601 ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt
     9661 tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca
     9721 aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat tcctaatatg
     9781 cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt
     9841 aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact
     9901 ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg
     9961 agtccagacc cactattgac agaccttcag cataagcttt ccgatattca actgggtacg
    10021 caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt
    10081 ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg
    10141 gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
    10201 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
    10261 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
    10321 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
    10381 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
    10441 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
    10501 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
    10561 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
    10621 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat
    10681 gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg
    10741 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
    10801 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag
    10861 ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt
    10921 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
    10981 ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta
    11041 aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag
    11101 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
    11161 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
    11221 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
    11281 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
    11341 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
    11401 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
    11461 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
    11521 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
    11581 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
    11641 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
    11701 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
    11761 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
    11821 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa
    11881 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
    11941 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
    12001 atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
    12061 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
    12121 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
    12181 aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt
    12241 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
    12301 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
    12361 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
    12421 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
    12481 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
    12541 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
    12601 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
    12661 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
    12721 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
    12781 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
    12841 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
    12901 tattgctttt tttcttcaag ataaacgttt taatcg
//
LOCUS       DAYYCM010000074        12786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000074 DAYYCM010000000
VERSION     DAYYCM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.076"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..2259
                     /gene="acm"
                     /locus_tag="ACHAY1_002325"
     CDS             94..2259
                     /gene="acm"
                     /locus_tag="ACHAY1_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8594283.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2531..3016)
                     /locus_tag="ACHAY1_002326"
     CDS             complement(2531..3016)
                     /locus_tag="ACHAY1_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8594284.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3106..3451
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3496..4137
                     /locus_tag="ACHAY1_002327"
     CDS             3496..4137
                     /locus_tag="ACHAY1_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8594285.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4156..4791
                     /locus_tag="ACHAY1_002328"
     CDS             4156..4791
                     /locus_tag="ACHAY1_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8594286.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4809..5624
                     /locus_tag="ACHAY1_002329"
     CDS             4809..5624
                     /locus_tag="ACHAY1_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8594287.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5943..7379
                     /locus_tag="ACHAY1_002330"
     CDS             5943..7379
                     /locus_tag="ACHAY1_002330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8594288.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7568..10000
                     /locus_tag="ACHAY1_002331"
     CDS             7568..10000
                     /locus_tag="ACHAY1_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8594289.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10230..11297
                     /gene="rlmN"
                     /locus_tag="ACHAY1_002332"
     CDS             10230..11297
                     /gene="rlmN"
                     /locus_tag="ACHAY1_002332"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8594290.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11287..11493
                     /locus_tag="ACHAY1_002333"
     CDS             11287..11493
                     /locus_tag="ACHAY1_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594291.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11490..12593)
                     /locus_tag="ACHAY1_002334"
     CDS             complement(11490..12593)
                     /locus_tag="ACHAY1_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8594292.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4372 a         2143 c         2730 g         3541 t
ORIGIN      
        1 attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt
       61 ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta
      121 tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat
      181 gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca
      241 gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca
      301 ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa
      361 aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat
      421 tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg
      481 caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc
      541 accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct
      601 tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt
      661 cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt
      721 gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac
      781 ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc
      841 cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa
      901 aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa
      961 caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt
     1021 gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt
     1081 acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca
     1141 aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc
     1201 ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat
     1261 gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa
     1321 ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag
     1381 aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg
     1441 agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca
     1501 gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat
     1561 gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat
     1621 tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa
     1681 gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa
     1741 cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa
     1801 gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg
     1861 caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac
     1921 gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc
     1981 catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta
     2041 ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat
     2101 tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct
     2161 aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc
     2221 tgtgctggga tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt
     2281 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
     2341 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
     2401 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
     2461 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
     2521 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
     2581 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
     2641 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
     2701 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
     2761 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
     2821 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
     2881 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
     2941 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
     3001 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
     3061 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
     3121 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
     3181 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
     3241 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     3301 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     3361 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     3421 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     3481 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     3541 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     3601 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     3661 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     3721 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     3781 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     3841 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     3901 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     3961 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     4021 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     4081 ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     4141 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     4201 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     4261 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     4321 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     4381 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     4441 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     4501 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     4561 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     4621 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     4681 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     4741 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     4801 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     4861 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     4921 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     4981 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     5041 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     5101 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     5161 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     5221 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     5281 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     5341 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     5401 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     5461 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     5521 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     5581 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     5641 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     5701 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     5761 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     5821 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     5881 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     5941 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     6001 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     6061 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     6121 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     6181 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     6241 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     6301 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     6361 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     6421 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     6481 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     6541 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     6601 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     6661 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     6721 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     6781 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     6841 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     6901 atacgacagg aaaaaaaagt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     6961 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     7021 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     7081 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     7141 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     7201 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     7261 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     7321 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     7381 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     7441 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     7501 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     7561 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     7621 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     7681 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     7741 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     7801 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     7861 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     7921 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     7981 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     8041 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     8101 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     8161 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     8221 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     8281 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8341 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8401 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8461 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8521 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8581 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8641 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8701 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8761 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8821 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8881 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8941 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     9001 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9061 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9121 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9181 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9241 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9301 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9361 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9421 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9481 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9541 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9601 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9661 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9721 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9781 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9841 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9901 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9961 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
    10021 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10081 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10141 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10201 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10261 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10321 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10381 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10441 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10501 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10561 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10621 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10681 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10741 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10801 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10861 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10921 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10981 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    11041 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11101 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11161 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11221 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11281 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11341 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11401 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11461 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11521 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11581 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11641 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11701 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11761 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11821 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11881 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11941 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    12001 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12061 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12121 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12181 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12241 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12301 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12361 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12421 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12481 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12541 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12601 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12661 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12721 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12781 taatga
//
LOCUS       DAYYCM010000075        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000075 DAYYCM010000000
VERSION     DAYYCM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.077"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHAY1_002335"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHAY1_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8594293.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHAY1_002336"
     CDS             complement(324..893)
                     /locus_tag="ACHAY1_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8594294.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHAY1_002337"
     CDS             1079..1981
                     /locus_tag="ACHAY1_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8594295.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHAY1_002338"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHAY1_002338"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8594296.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHAY1_002339"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHAY1_002339"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8594297.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHAY1_002340"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHAY1_002340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8594298.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHAY1_002341"
     CDS             8549..8734
                     /locus_tag="ACHAY1_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8594299.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHAY1_002342"
     CDS             complement(8796..9992)
                     /locus_tag="ACHAY1_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8594300.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHAY1_002343"
     CDS             complement(10162..11598)
                     /locus_tag="ACHAY1_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8594301.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHAY1_002344"
     CDS             11891..12700
                     /locus_tag="ACHAY1_002344"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8594302.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2673 c         2250 g         4011 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaat
//
LOCUS       DAYYCM010000076        12578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000076 DAYYCM010000000
VERSION     DAYYCM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.078"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..179)
                     /locus_tag="ACHAY1_002345"
     CDS             complement(<1..179)
                     /locus_tag="ACHAY1_002345"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594303.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYV"
     gene            complement(173..574)
                     /locus_tag="ACHAY1_002346"
     CDS             complement(173..574)
                     /locus_tag="ACHAY1_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8594304.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(761..2569)
                     /gene="uidA"
                     /locus_tag="ACHAY1_002347"
     CDS             complement(761..2569)
                     /gene="uidA"
                     /locus_tag="ACHAY1_002347"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8594305.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2592..4358)
                     /locus_tag="ACHAY1_002348"
     CDS             complement(2592..4358)
                     /locus_tag="ACHAY1_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8594306.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4371..5165)
                     /locus_tag="ACHAY1_002349"
     CDS             complement(4371..5165)
                     /locus_tag="ACHAY1_002349"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8594307.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5175..6551)
                     /locus_tag="ACHAY1_002350"
     CDS             complement(5175..6551)
                     /locus_tag="ACHAY1_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8594308.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6671..7318)
                     /locus_tag="ACHAY1_002351"
     CDS             complement(6671..7318)
                     /locus_tag="ACHAY1_002351"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8594309.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7402..8829)
                     /gene="uxaC"
                     /locus_tag="ACHAY1_002352"
     CDS             complement(7402..8829)
                     /gene="uxaC"
                     /locus_tag="ACHAY1_002352"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8594310.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8903..9982)
                     /gene="uxuA"
                     /locus_tag="ACHAY1_002353"
     CDS             complement(8903..9982)
                     /gene="uxuA"
                     /locus_tag="ACHAY1_002353"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8594311.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9985..11616)
                     /locus_tag="ACHAY1_002354"
     CDS             complement(9985..11616)
                     /locus_tag="ACHAY1_002354"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8594312.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11920..>12578
                     /locus_tag="ACHAY1_002355"
     CDS             11920..>12578
                     /locus_tag="ACHAY1_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8594313.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     Y"
BASE COUNT         4045 a         2506 c         1641 g         4386 t
ORIGIN      
        1 tctacatagg taacgaattg acctacctcg ttaaatccat ttgtcaaaca taacttagcc
       61 atataagaat gataatgttt gagtatcatc ttgatcgaaa ttggatctcc ttctagagca
      121 gataagataa tggatagtgg aactaatagg tactgccgct ttttagattt atctaccata
      181 ggtcataccc cattttcaat tgttcaactg ccttctttcg ttgataccag ataccacttg
      241 tgctcatatt gagaatttta ccgatttcag catcattaaa acctgcaaaa taataaagta
      301 ggataatctt tcttcttatc tccgataact gatcaattgc tgctgataac ttttgatctg
      361 aaattaatat ttccattcca aataagaaat agacatcttg ttcatcaaaa tgagtttggg
      421 aatacctgcc ttcatttgaa tctgctaatg gttcctcatt atcctttatg ttagcgtttc
      481 gttttcgaat agacttagct tcgtttcgta atactttttt acagtaggca tcaaattggt
      541 gttgtataga tatgtcattg taatgttgtt gcatgataat atgctccttt ttcccccttt
      601 ccactaatac aacaatcaga aagccccttt ttttcgaaaa aactagaaaa aaaacataaa
      661 aatatttatc attctttaaa cagaaaatct ttctataaaa aacaaaaaaa aacgattagg
      721 ctaggctaat cgttttttga acattcttaa aaaatccaaa ttatttttta taactgaaat
      781 tgggtattga tgaccatcgt ttctgtagat agtaagcaat cattttaggt tttctatctc
      841 tagtaaagat tcctttctta tttccttgca ctcttaaaat cccttgactt gttgcaaaat
      901 ctgcaaaatt ccacacttgt tcaccaacaa aattttcaca tgtatctatt acttcgtgat
      961 ttgcttcgta ataagccact tgatactctt ctgtaaacat tgttggtact gtatcatgaa
     1021 atccagctac tgtatctgct ccatattctg taaacattat tggcgtatca ggacatcttt
     1081 ccatccatcc atctagctct tttttcaaca gatattttgc atgtgccaga tcccctcccg
     1141 aaacatacca accataataa cgatttaaac ataaaacatc gacaatatct cctaccttac
     1201 aaacatctgg cgttgatttt agataagtaa cgattgttac aggtcttttc tgcggatcaa
     1261 gttctttagt taattcaact aaaggttcaa aatattcttt agcaccttcg gaatccgaat
     1321 ctggttcatt tgcaatagac cacattacaa cacttgggtg gttcttgtcg cgttctatca
     1381 attccttgat caccgtttta tgagaatttt ctgttcgtat ctctttccac gtattatgtt
     1441 tttccacatt atcatctaat aaagtggcca tgaaatttaa atgtaaccct actgcaggta
     1501 cttcatcaat aactacaata ccttctcgat ctgctaatcg cataatttct tctgagtaag
     1561 gataatgtga tgttctaaaa gaattagcac ctatccattt cattaaatta aaatctttaa
     1621 tatttacagc ctcattcaaa ccacgtccat taacatacga atcctcatgc ttaccaaatc
     1681 ctttaaaata aaaaggttta ttattaatca aaaatttgcc attggtaacc tgaacactgc
     1741 gaataccaaa ttcttctgta taggtatcga tcactttatt atcatcaatt aaactaattt
     1801 tcatactata taaataagcg tctagaggtt cccaaaaaat tggattctta agctgaatca
     1861 ttcctttctt tccttcagct tcagctacaa gttgagcctc ttcgtcatat atcgctatta
     1921 agatttttcc ggtccattct gtatcgatat tgaattctac acttgcatcc aaatcagtta
     1981 ttcttgtatt aatagtaata tcacttatat agtctttcgg agttgtatat aacttaacag
     2041 gtctatgaat acctgcataa ttgaagaaat caaaattaac taagttttcc ttttttatat
     2101 ccccattttc ctgtactgtt tccttcataa tccccacagg taaagtacta tcgtcaataa
     2161 tattgtttac agcaactgta attcgattat tcccgtcttt tacaaaccga ttaatttcag
     2221 actcaaatgg tgtaaaccca cctttgtgtt gagtaataaa ctttccattc aaaaaaactt
     2281 ttgcttcatg tgttgcagat ccaaaacgaa gaactaatct ttcttctaat aatcttttag
     2341 gaagagtcac ttctttttca taccacaccc atccaacatg atttctaatg ttctctgttt
     2401 caaataaatc attataagaa gagggaactg aaatcggata actctctttt tcatttattg
     2461 gttctaaagc aagttcttcc tgtaagtcat catttgtatc taatttaaat ctccagagac
     2521 cgcttaagtc aattacttgc ctcgtctctg tcataatggg atacaacata taaaccactc
     2581 ctatctaaat attaaataaa tgtatccttt aaaccacaat aagcatctat tggacttact
     2641 cccttaaatt cagaaagacc cattagtttg tccaccaata tttctaaagt ttcttgtttt
     2701 ccatcataag tattaatata agtctttact tgaggtacat cagccaaatg aaacggacac
     2761 tgtacagata caaaaattgt tggaatttca tgaatgtaaa acggtatatc cggagttcct
     2821 ttacttgctg gccagacaat tcgttgaact gtgcctggat taacattagc tatattaatg
     2881 atcaagtcat attttttagt taaattttta attggtctct tagcagcata tattccagct
     2941 atatttttaa ttttttcttc ttgcgataat cctttcaatt cttcttcagg tgaaatgtat
     3001 ttttcaactt caaatccttt tgctgacaaa atctgtttca ataaatcaat ggcttgaggt
     3061 ctatttccag caactacttc accaaaccca ccttttaccc catgtacatc tactagtaaa
     3121 atccttttat atttttctgg tacaataggc caaatatctt gtttatcttt aactaatgta
     3181 attgcttggt cagccacctc tttaaaaatt gatttatttt caattaaacc aattttttcc
     3241 agagcttctt gcttaggtaa taaaatttgt tcttttgctt ttttgtgtaa tcccaattta
     3301 gcttttaacc ctaaaattct ttttaaggca tctaataacc gatcatcagt gataattcct
     3361 ttacgatagc cttccatcat ataattaaag tcttcatctg gatcattaaa aaatagaaat
     3421 agatcacatc cagctgcaat agaagtgggc aataattctg atcgtttcat tgccccagtc
     3481 attcctaaca tatgactagc atcagtaact actaatccat taaatccgag ttttcctcta
     3541 agtaagtcag tgattaattc ttttgataaa gttgcaggaa gtaaatcttc atctttcaga
     3601 tcaggattaa aatgttttga ataagctggc atatggatat gacctgccat aattgaaggt
     3661 aatccagctt ctattaagcc tgagtatact tttccaaaag aattatccca ttcttccgta
     3721 cctaaactat taatactaaa agatagatgc tgatctcgtt cgtcaatacc gtcaccaggg
     3781 aaatgtttag ctgcaggcaa aattccactc tcctgaatac ctttcatata agctaaacta
     3841 agttctaaaa ctttatcagg gttttgtcca aaagatcgac tagaaatgat aggatttctc
     3901 caatttctat taatatctac aataggagca aaactccagt tacatccaat agccgatgct
     3961 tcaatcccag aaactctccc taattcataa gcatatttcg catcatttgt tgcaccaatt
     4021 tttacttcta caccaacttc agtaccatct gtacaagctc cgttaccacc tgcttccgta
     4081 ttagcagcaa ttaatagagg aattttactt tctgtttgta agatatagtt ttgatcatat
     4141 acctcatcag cagttcctgg attatatcgt acagctccaa tatggtactg attgaccata
     4201 ttactcaagt aatttttatc acgactagat cccatattaa caaacaattg acctattttt
     4261 tcttccaaag tcatagaatc aatcgtctct tcaatccaag aaatagcatc ttcatctaaa
     4321 ttatatggtt tttttcgtaa atctactttt atagtcattt tctttctcct ctacttatta
     4381 gtaagatttt tccaaaaagt atctatatat ttttgttcaa tatctacata atctcccgat
     4441 aaacatttaa ataaaacatc agttcttaag ttttcccatg attctttttc tttattgacc
     4501 aaaataggat aaaagaaaac tttattttta tttgcagctt ctagatcacc aggagaatca
     4561 ccaatcatta atatcttatc caaatcacaa ccattttcaa ttacacttcg aataatatct
     4621 tctttttttc ctttatcttg acaacacagt tcatcaacta atgataataa ttcatgtctt
     4681 tcccattctt cttgaacagc ttccttatta gctgagctta caataattat cttcccatat
     4741 tttttcagag cttttatagt atctctagca cttaaaaagg gtttatctaa tccttttgct
     4801 tgactaatct gttgattgac ttcttccgac cattttaaag cttttaataa atcctctgac
     4861 tgagaatatt ctatttccct cttcaaagat ggattagaca attcttttgt tagagatacc
     4921 cagtgcttta atcgattaat cgatgttaca tcattttttt ctaatgtttt aactaaacct
     4981 aaaaatctgt ttattccacg agtctctgaa tataaattaa tattattcca atcctgtagg
     5041 aatttttctc tatgatgaac atcaaaaatc tctgcagcaa ttggtccgaa aaagagattg
     5101 tgtttataag tcatcgtatc cataacacag ccatctgaat caatacaaaa tatccaatta
     5161 ttcatatttc tctcctattt atgtttttgt tttaaaagct cgcaattttc taagacttta
     5221 tctttagata atggataaag gaaaaataaa attaatccta caatcagaaa acaaattgat
     5281 ggaattaatg ttgaagcgtt atataacttt tctaaaacat ctcccgtttg tacatttgct
     5341 aaagagtcat agcctataat agaaagagtc caacctccga cgcctccagc tagagcttga
     5401 cctatttttc tagcaaaaga atataccgca tatacggtcc catcttctct ttgatcagac
     5461 tttacttctt gatcatcaat aacatcagta atatttgccc aaataacagt attaaacaca
     5521 cccaaaccca tatagccaac cgaactaaga attaagaata catacatatt actagctttt
     5581 atcaaaaata agatcaaata tactattgct gaaaagaaca ttcctactga tgccagttct
     5641 ttttttccat attttttact taaaaacaaa gttaaaggcg aaacaactat taaaaccaaa
     5701 ataggattga taaaattcat taaaactatt ccctgtgcat cattatagta atcaggaaat
     5761 acataattat tcatactcat aattaataat tgagcaagga gtaagaaaat agccgcacca
     5821 attatagaaa gtaatgccct atttttaact aaccctttta aagattttaa caatgaaact
     5881 ttgtattgag attggtcaat tcttactcgt tcagtagtta gtttataaca tattaaataa
     5941 caaataagtg cacatatcga aaatattccc gcaacaattg taaaagttga tccaccacga
     6001 actatttgag cgcctgaatc atctcttgta taaataaata atggtgttaa tacaccaatg
     6061 accaaacttg ctaaagtagc tccaacattt cggaatgtcg atagcattgt tctttcttct
     6121 gtaatagaag taatagctga tgccattgat ccataaggaa tatttataga tgtgtaaaaa
     6181 atactccccc ataatatata cgtaacatac atgtatacta ccttaacgct catagaagct
     6241 gaaatcatag aagtttgata cattaaaaaa cttgctaaag cgactggacc actcattctt
     6301 aaaatccacg gtttaaactt accctgctta gttgctttcg atctatcaac aatacgcccc
     6361 atagttacat cagtaactgc atcgataaat cttgctacta aaaataatgt tccaacggca
     6421 gctccgctta tccccaatac ttttgtataa aagatcatta aaaaagaact agcaaaaatg
     6481 aaagtaaaat cgtttccaaa atctccaaac atatatccta ttttgtctct tataccaaat
     6541 tttctttcca tacaattctc tcccttctgt tgttaagcgt tttcattctg atgaaagtac
     6601 gtactatact ctgaagcgta aagagtcagt atttattttt tacatttcag agtattattt
     6661 gttttttctc ctaataattc tttatctcat aaaacttatc tatatattta ctagctactc
     6721 gacttacttc ttcaaaatca cctttttcaa cagaagccaa taaattccca cctacaccaa
     6781 ctgcaacaac tcctacttca aaccaatctt ctaagttctc tatacttact ccgccagtag
     6841 gcattatatt tacgtgtggt aatggagctt gataggcttt tacaatggat ggaccaaaag
     6901 cagaacctgg gaatagttta atgatatcta cgccagcagt aagtgcctcc tttatctcag
     6961 taattgtcat acaacctggt aaataaggaa tttgatataa attacaaatc tcagctgttt
     7021 gtcgatcaaa agatggacta acgatatatt ctgccccagc cataattgcg atacgagcag
     7081 taaacgcatc taaaaccgta ccggctccga ttattacatc tttttgatca gcatattttt
     7141 tagcaatttc tttgattatt tcttcagcat taggaactgt aaaagttagt tcaatccctt
     7201 taattccacc cgcaataatt gaatttattg ctttaatggc ctcatcctta tttttcccac
     7261 gtacaaccgc aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat
     7321 ctatctcctt cctttatgga tacatacttt gtctagctgt actaacttac aaagaataaa
     7381 tttaagcttt tatttcaagt attattttct acttccgatt tcaaaaaaat taaaataatt
     7441 atacgcatta cgataacaaa tattctccac tatctgacta agatacattt catcatctgg
     7501 aattcggcct gctttaaccc actcgcttag taattgacat aatactcgac gaaaatactc
     7561 atggcgagga tagcttagaa aacttcgaga atcagtcaac atacctgtaa aattcgacaa
     7621 caaactttgt tgtgccatcg tagtcaactg ttgacgcatt ccttcagctg tatcattgaa
     7681 ccaccaagca catcccaatt ggattttttg aatcataccc ccttggaagc tttgcattgc
     7741 tgttgctagt ggaagccaat cagttggatt taatgaatat aaaattgttt ttggtaagca
     7801 cccactcttt tcagcggtat cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc
     7861 ccccatcgaa tcaccaccga catctactcc aatttgttcc tttaatatac ttgaattatt
     7921 tcttaaagca tttaaatgca tttgcatcgt ccaaccatat cttgtatact cagacatcaa
     7981 atgtaattga atacctgtaa caaattgtgc aatttctgat aaatggaaat ccgtttttcc
     8041 tgctaaagct tttattaaca gattgtcgag tattttttca tctgctattt cataaaaata
     8101 agtattcaaa ccgtgatctg ctaatcgaca gccaatttga tgaaaatgat ccactcgttg
     8161 gctcaatgct tgcttcaacc cattaaagtc tttgattggt atagccgctg tttgactaag
     8221 ttttaaaata tagttcgaaa agcctgatga tttgatactc cagaccttat ctggacggaa
     8281 agtaggtaat accttgaaag aattatcctg tttagaaagc tcagtatgat accacaagtc
     8341 atctgtagga tcatccgttg tacagatggc tttcacatcc atcatctcca acaattcttt
     8401 cgggcgatag gaatccgttt gaattttttc gttagcacgt cgccaaattt ctggtgcatt
     8461 cttctcagaa attgtcaaat caatatcaaa gtaacgttgt aattccaaat gagaccattc
     8521 aaaaatagga ttacctggcg ccttctccac tgtttttaca aaagctaaaa atttttcata
     8581 atcatcacct ggaccactga tcaactcttc ttctatacca ttagctcgca tcaatcgcca
     8641 tttataatga tctccagctc ctttatcatt taaccatacc tgagtcaaat tcttgtattg
     8701 cttattttct agcaaatatg ctgggtctaa atgacaatga tagtcgataa ttggttggtg
     8761 ttttgcaaat ttatgaaaaa gtcttttagc tatttcattc tctaataaaa agttcttatc
     8821 taaaaacata tttaaacact cctttccttc cgttagtttt tagaagagaa aatttttcct
     8881 ctaaataaac tcccttatat aattatttac tcgccccctt ttcaagcgct tcccaaatac
     8941 catttaaata aactgcacct agagctcggt catataatcc ataaccaggt ctaccggttt
     9001 ctccccaaat catccgacca tgatctggac gaatatagcc attaaatcca ttatcgtaca
     9061 ttgtttttaa tatcttatac atatctagag aaccatattc tgatttatgc ggcgcttcgt
     9121 aaaaatcgag tccattgtca cttaaatatt tgatattacg aacatgtgca aaaggaatcc
     9181 gatcccgttg acaaaattca gatagaattt catagacatc attatctggt cgttctgcaa
     9241 tacttccagt acataatgta ataccattag aaggagaatc cactacatta cacaaccaat
     9301 ctaaatcgtc acgattttta acaatacgag gtaatccgaa aatggaatat ggtggatcat
     9361 ctgggtgaac tgccatttta atcccaactt cttcacatgt cgggataatt gctttgagaa
     9421 aatatgcaaa gttctctcgt aatttctctt catcaacttc tttataggct tctaataatt
     9481 gcttaatttg tgataaccga tgaggttccc aacccggcaa ttcaaaatta ttagtagaat
     9541 tttctacttc tctaataatt tcctccggat cagtagggat attttttgaa atataggcta
     9601 acgtagaaga cccatcatct aaacgataat ccaaatccga ttttacccaa tcaaatactg
     9661 gcataaaatt ataacagata acctcaatcc caaattctgc caaattgcga atcgtctgtt
     9721 tataattctc aatgtattga tctcgtgttg gtaaaccaat tttgatatca tcatgaacat
     9781 taacactctc aataactttc aaggtaagtc cattggcttc gacttcatct tttaaagatt
     9841 taattcgttc tctcggccaa acctctccgg ctggtatatc cattaatgtt ccaacaattc
     9901 ctttcattgc tgggatctga cgaatttctt gtagtgagat tttttcttca tttgccccat
     9961 accatctaaa agtcatttcc atagttactt tcctccaact tctgttatat atttattgat
    10021 tgttttacga actgcttgag gacctactag catttcgcaa aagatttttt cagttttctt
    10081 tcctacccca acttgatata aatctaaacc aaagatttct ttattttcta aaatttcctg
    10141 taccgcacaa tgaatctttg aaggatcttg ttcacctaat ttcaatactg ataatttatc
    10201 ctgcaactct accaataacg gatcaggact tggcacaaaa ttttcccctt gatcatcgac
    10261 tgccagtaaa taacgtaacc aagcagcaat agcgatagga ataaatgtta gattttctgc
    10321 atttccgtct ttttcgacat actttttaat tgtttcacca tagcgaatcg ctactttttg
    10381 agaagtatct gttgcaattc gttgtggtgt atctggaata tagggattag gcaaacgttt
    10441 attaattact tcatcaataa attgttttgg atcaataatg cctggatttt caactactgg
    10501 taaaccttct aaataaccta ttcctttgat taaaccaacc aaatcatcat cctgcatctc
    10561 tgctgaaatt gattgatatc ctagcagaca acctgttact gccaaagcag tatgaagtgg
    10621 attcaagctt gtcgtaacct tcatgatgtc tgctttatca accgtctctc tatctgtcat
    10681 aatcacacca gccttttcca atttaggccg gccatttgga aaactatctt caataactaa
    10741 ataatgaact acctcagtat tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat
    10801 gatatctaaa tcctcgagcc ccatcatcaa tagatcttcc tttacattct ctgaaggatt
    10861 tggcgtaatt cgatcaatca tactccaagg aaaagtcact ttttctttat cctttaaata
    10921 agcaataaat ttttcagaaa catatccttc tttttgccaa gcttctgcaa tcgtaaaaat
    10981 actatcttga aaacgttgac cattctgtga gaaattatca gtagaaacca tcgcaatcgg
    11041 caactctcca gcattaaatc tgctatataa caaggatgtt acaatactca tcgtatgttg
    11101 aggattgcta ggtccagatt tgatatcagc tttgactacc gaaaagaatt gccctttaga
    11161 atcttttaaa ctgtaacctt tttcagtaat agtaaacgtt gtaaactgta atttaggatc
    11221 ttcaaaatat ttaaccactc tttgatagtc cgctatattc ttcggattgc aatataaact
    11281 atcagccgtt actgccaaaa gtcttttttt gagttcacca tctccactca taacaatttc
    11341 caaaatgtca ttgttatagt catgataagc tttttccact acttcttcat caaatgtttc
    11401 acacacaact actcctgttt ctaattcatt ttgatctgct aagcattgag caatttctgc
    11461 atgaaatcct cgataaagat ttcctccacc aaaatgaagc catactggat gttgctggcc
    11521 ttcttgctta acttctttga catcaaaaca cggcagtaga atctgcattt cattgaattc
    11581 ttcgatagaa ttttgccaat ttttaatcga aaccataatt aacctcctca aactgatatg
    11641 ttagttttga tacattttat attttaaaac ccattgtaac cgttgtcaat ataaaaaggg
    11701 aataaacttg cttttttcta ccatatcaga atgattgtat tttttattca gaaaaataaa
    11761 atactccttc tttttggact gattttatcg aaacttattt tttctttttt tcatgtattc
    11821 ttttattttt ataactagtg tattacttca aaatttgata ttatatggca tgaacagaat
    11881 taaaatatca gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc
    11941 caaaatcaag cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga
    12001 aaaaaagtat cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt
    12061 gaagcactga ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat
    12121 tatatctcat taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg
    12181 gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta
    12241 gaaactataa tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt
    12301 cataatgata atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg
    12361 caatggttag aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg
    12421 gaaggattaa agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca
    12481 aaacaagatc cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
    12541 gagcaaaata ttgttttagc tactcatcca aaatattt
//
LOCUS       DAYYCM010000077        12393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000077 DAYYCM010000000
VERSION     DAYYCM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.079"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="ACHAY1_002356"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="ACHAY1_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8594314.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACHAY1_002357"
     CDS             complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACHAY1_002357"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8594315.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACHAY1_002358"
     CDS             complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACHAY1_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8594316.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACHAY1_002359"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACHAY1_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8594317.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="ACHAY1_002360"
     CDS             3719..4198
                     /locus_tag="ACHAY1_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8594318.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="ACHAY1_002361"
                     /pseudo
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="ACHAY1_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5499..6284
                     /gene="phnX"
                     /locus_tag="ACHAY1_002362"
     CDS             5499..6284
                     /gene="phnX"
                     /locus_tag="ACHAY1_002362"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8594319.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6630..8321)
                     /gene="argS"
                     /locus_tag="ACHAY1_002363"
     CDS             complement(6630..8321)
                     /gene="argS"
                     /locus_tag="ACHAY1_002363"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8594320.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="ACHAY1_002364"
     CDS             complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="ACHAY1_002364"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8594321.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9805..10824)
                     /gene="argF"
                     /locus_tag="ACHAY1_002365"
     CDS             complement(9805..10824)
                     /gene="argF"
                     /locus_tag="ACHAY1_002365"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8594322.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="ACHAY1_002366"
     CDS             complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="ACHAY1_002366"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8594323.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3422 a         2556 c         2203 g         4212 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttaatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgctcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccacccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgca tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc atattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattaa
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaattttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa ctcctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcggc tcctttgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatgg
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 gtaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccctctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaaaagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgattcgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactaga
     5041 aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtactac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa cggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgcgaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gactggattt actgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaagggta ttcaccagac ttcatggcaa
     5941 cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaaagat cgtttaaaac
     6181 agtctgttgc tcttagattc caacaagctg gcgctgatat ttgtgtaaac aatttaaaag
     6241 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaatca
     6301 gaggtcgtga aaaagctgta ctgcatcaag taataagaac agccaaacaa aaatagctcc
     6361 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6421 tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tacaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggcg catatcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tcttcatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggaca
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatatt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgttcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tctgataaaa aaacaatcca gatggcctaa aggagaagaa ataccacctg
     8701 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8761 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8821 agcgtttggt tttgcttcaa caaatgcaat agctgcctct actttcggaa gcatgcttcc
     8881 tggagcaaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8941 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     9001 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     9061 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     9121 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9181 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9241 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9301 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9361 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cattttgtaa
     9421 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9481 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9541 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9601 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagattg cattaccccc
     9661 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9721 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9781 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9841 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9901 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9961 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
    10021 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
    10081 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
    10141 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10201 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10261 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10321 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10381 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10441 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10501 gaattcgatt ccatcaaaca tacgacctag tactttagct gtatcttctg tggattcttt
    10561 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10621 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10681 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10741 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10801 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
    10861 ttaaagagga aatcccgacg gcagctgaca gtaagaccgc cgtcaggaaa caaattactt
    10921 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacggc ctcgtgacaa
    10981 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
    11041 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
    11101 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
    11161 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
    11221 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
    11281 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
    11341 cattgcacgg ttgtttggaa ttttgattgc taacactttt tcaaatcctg aattttttgc
    11401 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
    11461 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
    11521 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
    11581 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
    11641 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
    11701 aaatggatag tcatcgtctg ctgacagatc gtatagactg ctagaacgta cttcaatatc
    11761 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
    11821 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
    11881 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
    11941 gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca aagttgtcat gttcttcttg
    12001 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
    12061 attttccact tcttgtccag gccttttcag taaaactgtc tttaatttcc ctatttcaga
    12121 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
    12181 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
    12241 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg taatttattc
    12301 attttttatt ctgtatacac tttcaaaaaa atatttattc attgaaaaaa attttttgaa
    12361 taaattaaaa aaataaatta tattagcgtt gtt
//
LOCUS       DAYYCM010000078        12167 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000078 DAYYCM010000000
VERSION     DAYYCM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.080"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHAY1_002367"
     CDS             <1..610
                     /locus_tag="ACHAY1_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8594324.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHAY1_002368"
     CDS             723..1001
                     /locus_tag="ACHAY1_002368"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8594325.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHAY1_002369"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHAY1_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8594326.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHAY1_002370"
     CDS             1458..2213
                     /locus_tag="ACHAY1_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8594327.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHAY1_002371"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHAY1_002371"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8594328.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHAY1_002372"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHAY1_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8594329.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHAY1_002373"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHAY1_002373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8594330.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHAY1_002374"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHAY1_002374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8594331.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHAY1_002375"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHAY1_002375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8594332.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHAY1_002376"
     CDS             6950..7492
                     /locus_tag="ACHAY1_002376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8594333.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHAY1_002377"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHAY1_002377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8594334.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHAY1_002378"
     CDS             9085..9987
                     /locus_tag="ACHAY1_002378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8594335.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHAY1_002379"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHAY1_002379"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8594336.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHAY1_002380"
     CDS             11430..11852
                     /locus_tag="ACHAY1_002380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8594337.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4043 a         2158 c         2687 g         3279 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagag
//
LOCUS       DAYYCM010000079        12142 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000079 DAYYCM010000000
VERSION     DAYYCM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.081"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACHAY1_002381"
     CDS             <1..565
                     /locus_tag="ACHAY1_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8594338.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /gene="fic"
                     /locus_tag="ACHAY1_002382"
     CDS             966..1571
                     /gene="fic"
                     /locus_tag="ACHAY1_002382"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8594339.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="ACHAY1_002383"
     CDS             1587..2159
                     /locus_tag="ACHAY1_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594340.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="ACHAY1_002384"
     CDS             complement(2411..2668)
                     /locus_tag="ACHAY1_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594341.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="ACHAY1_002385"
     CDS             complement(2794..3609)
                     /locus_tag="ACHAY1_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594342.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="ACHAY1_002386"
     CDS             4126..5451
                     /locus_tag="ACHAY1_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8594343.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="ACHAY1_002387"
     CDS             5444..5794
                     /locus_tag="ACHAY1_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594344.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5791..5961
                     /locus_tag="ACHAY1_002388"
     CDS             5791..5961
                     /locus_tag="ACHAY1_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594345.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACHAY1_002389"
     CDS             complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACHAY1_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8594346.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="ACHAY1_002390"
     CDS             complement(7112..8065)
                     /locus_tag="ACHAY1_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8594347.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="ACHAY1_002391"
     CDS             8677..10170
                     /locus_tag="ACHAY1_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8594348.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="ACHAY1_002392"
     CDS             10305..10601
                     /locus_tag="ACHAY1_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8594349.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="ACHAY1_002393"
     CDS             10702..11553
                     /locus_tag="ACHAY1_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594350.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11786
                     /locus_tag="ACHAY1_002394"
     CDS             11574..11786
                     /locus_tag="ACHAY1_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594351.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4169 a         1864 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac ga
//
LOCUS       DAYYCM010000080        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000080 DAYYCM010000000
VERSION     DAYYCM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.082"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHAY1_002395"
     CDS             <1..465
                     /locus_tag="ACHAY1_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8594352.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHAY1_002396"
     CDS             complement(1080..2120)
                     /locus_tag="ACHAY1_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8594353.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHAY1_002397"
     CDS             complement(2469..2795)
                     /locus_tag="ACHAY1_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594354.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHAY1_002398"
     CDS             complement(2782..3570)
                     /locus_tag="ACHAY1_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8594355.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHAY1_002399"
     CDS             complement(3938..4228)
                     /locus_tag="ACHAY1_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8594356.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHAY1_002400"
     CDS             complement(4364..4543)
                     /locus_tag="ACHAY1_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594357.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHAY1_002401"
     CDS             complement(4540..4890)
                     /locus_tag="ACHAY1_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594358.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHAY1_002402"
     CDS             complement(4883..6208)
                     /locus_tag="ACHAY1_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8594359.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHAY1_002403"
     CDS             6658..7272
                     /locus_tag="ACHAY1_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594360.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHAY1_002404"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHAY1_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHAY1_002405"
     CDS             complement(7883..9202)
                     /locus_tag="ACHAY1_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8594361.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHAY1_002406"
     CDS             complement(9336..9944)
                     /locus_tag="ACHAY1_002406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594362.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHAY1_002407"
     CDS             complement(10582..10839)
                     /locus_tag="ACHAY1_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8594363.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHAY1_002408"
     CDS             complement(10832..11101)
                     /locus_tag="ACHAY1_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8594364.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHAY1_002409"
     CDS             complement(11232..11411)
                     /locus_tag="ACHAY1_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594365.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHAY1_002410"
     CDS             11509..11745
                     /locus_tag="ACHAY1_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594366.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYCM010000081        11894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000081 DAYYCM010000000
VERSION     DAYYCM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.083"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..285)
                     /locus_tag="ACHAY1_002411"
     CDS             complement(79..285)
                     /locus_tag="ACHAY1_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594367.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(301..915)
                     /locus_tag="ACHAY1_002412"
     CDS             complement(301..915)
                     /locus_tag="ACHAY1_002412"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8594368.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1062..1745)
                     /locus_tag="ACHAY1_002413"
     CDS             complement(1062..1745)
                     /locus_tag="ACHAY1_002413"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8594369.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1854..2312)
                     /locus_tag="ACHAY1_002414"
     CDS             complement(1854..2312)
                     /locus_tag="ACHAY1_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8594370.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2396..4159)
                     /locus_tag="ACHAY1_002415"
     CDS             complement(2396..4159)
                     /locus_tag="ACHAY1_002415"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8594371.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4380..5840)
                     /locus_tag="ACHAY1_002416"
     CDS             complement(4380..5840)
                     /locus_tag="ACHAY1_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594372.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6048..6974)
                     /gene="glsA"
                     /locus_tag="ACHAY1_002417"
     CDS             complement(6048..6974)
                     /gene="glsA"
                     /locus_tag="ACHAY1_002417"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8594373.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6997..8424)
                     /locus_tag="ACHAY1_002418"
     CDS             complement(6997..8424)
                     /locus_tag="ACHAY1_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8594374.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8455..9669)
                     /locus_tag="ACHAY1_002419"
     CDS             complement(8455..9669)
                     /locus_tag="ACHAY1_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8594375.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9755..9982)
                     /locus_tag="ACHAY1_002420"
     CDS             complement(9755..9982)
                     /locus_tag="ACHAY1_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594376.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(10010..10252)
                     /locus_tag="ACHAY1_002421"
     CDS             complement(10010..10252)
                     /locus_tag="ACHAY1_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8594377.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10284..10841)
                     /locus_tag="ACHAY1_002422"
     CDS             complement(10284..10841)
                     /locus_tag="ACHAY1_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8594378.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10854..11042)
                     /locus_tag="ACHAY1_002423"
     CDS             complement(10854..11042)
                     /locus_tag="ACHAY1_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8594379.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11055..11621)
                     /gene="amaP"
                     /locus_tag="ACHAY1_002424"
     CDS             complement(11055..11621)
                     /gene="amaP"
                     /locus_tag="ACHAY1_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8594380.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3483 a         2454 c         1945 g         4012 t
ORIGIN      
        1 agttgcacca ggattctttg ataatttttt agttttatgt gtttttttat atgttttttc
       61 acttttcgga ataaatattt atttgttaaa gtaattaaac aatccgctaa tcgtttgatc
      121 tggcatttct atttccttac catctaaagt aacagtagta acgacaaaat ggtcttgcaa
      181 tggcggaact tctaagtact gcccttgcag aaaatcattc actgtttcat attctttttt
      241 catttctttt gaatctgtcg atggtttata gcttattttt aacataacta gtgctccttt
      301 ttattcggga cgtgcaatcg tgaaaatttc tcctattgct gcataatcat ttcctgccaa
      361 acggcttaca gcattcaatt ttcttggatc gatccttcct gtcttatcat catagactgt
      421 ttcatttaaa tgatatttct gtacttctag caaaaataaa tcagctgtta ctttattttt
      481 attccgtatc tcgacatgtt catataagga tgtttcaaaa cgaacttttg cttctgtcaa
      541 tgctggtact gaaatgatta ctgaatcagt taaagtcaag tcagtcaggt acagttcgct
      601 ttcctcatta ggcagattgg ctgctgtttt atttacttct tctacgttgt cctcatcaag
      661 gatatggata acagcttctc ttgtctcaag aatattcttt gctgtgtctt ttactgcatc
      721 ttgttttctc tggatcgcca aggataagat gggtcgattc gaactgacga tattgaagta
      781 actgaagggt gcgatattca ctatatcttt tgctgattta gtcgttacta ctgctactgg
      841 tctagggatg actgaaccaa tcagcaactt gtaattttca cgttctgtca attctgaagg
      901 actccgttca aacatggttt cgccttcttt ctataaaatt cgttaaaaag cagatcttga
      961 agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa actatctatc
     1021 aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt tgctttactt
     1081 gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga tccaactacg
     1141 actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc agtatgtcct
     1201 tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc aaaatcaggt
     1261 gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa
     1321 ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat agctaaccga
     1381 ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg acgtttacga
     1441 gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc cacttctttt
     1501 tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat ccctattacc
     1561 ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc tcgaatacca
     1621 actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga actgtgtagc
     1681 ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg ttccagtgta
     1741 tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa aaatgtaacc
     1801 cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc ttactaaaag
     1861 aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc tatgttcaac
     1921 gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata agctaatgtc
     1981 cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa aggcaagatg
     2041 gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag gaccacgtct
     2101 aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta accacttgat
     2161 gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat tttttctgta
     2221 tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt ttcttggaac
     2281 tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct ttttataatg
     2341 aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata atgaattatt
     2401 gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt
     2461 cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca aaatttaata
     2521 cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct cctgacaaac
     2581 taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct aatgtttcta
     2641 cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct ttttttctaa
     2701 aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa attgctactt
     2761 ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg atagctgtac
     2821 catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg
     2881 cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat ttgatgttcg
     2941 ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca ctagctgaga
     3001 tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa tcatgtgata
     3061 attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga cgtactccct
     3121 gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc acgtcaaaat
     3181 aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat
     3241 tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa tccatgatga
     3301 tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac agatcaaatg
     3361 gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg cgaccttttt
     3421 ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca aataaacgga
     3481 tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc attgctttaa
     3541 ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt ttatataaac
     3601 tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt agattggatt
     3661 gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg gagccgtcac
     3721 cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta gctaaacctg
     3781 ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat
     3841 gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca ttttttcgac
     3901 cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt ttatcctttg
     3961 gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga ttcggaccaa
     4021 cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct tctgccaact
     4081 gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt gcatccaata
     4141 catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt taatgaaaat
     4201 agatttgtta cactttatct attctactac ttagagttga ctctaagtca atagcatttt
     4261 aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc aggactcaaa
     4321 taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat
     4381 caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa atggatgttt
     4441 gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc attttgatag
     4501 ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc aggcaattct
     4561 tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc atacaaatga
     4621 aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa aatttctatt
     4681 tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa ttttcgaatg
     4741 aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc caatatttct
     4801 acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct ttccggtaaa
     4861 aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg cttgaaaata
     4921 aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa ttcattcagt
     4981 gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct taacagctcc
     5041 atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata gacatcttct
     5101 tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa ttgaaataac
     5161 ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa tgcctttctt
     5221 ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga atatcttctt
     5281 ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc tacatacgga
     5341 tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa gatgttccgc
     5401 gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc tttaaaaaaa
     5461 tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc aagatcagcc
     5521 agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat
     5581 tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta
     5641 tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt
     5701 tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg
     5761 tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc
     5821 tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta
     5881 atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc
     5941 aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg
     6001 tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg
     6061 tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca
     6121 aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc
     6181 aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat
     6241 tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta
     6301 atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt
     6361 gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc
     6421 ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata
     6481 tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca
     6541 cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca
     6601 atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga
     6661 tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt
     6721 gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt
     6781 gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta
     6841 tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg
     6901 taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat
     6961 tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt
     7021 atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag
     7081 aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat
     7141 acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt
     7201 tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata
     7261 gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc
     7321 aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc
     7381 aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa
     7441 atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac
     7501 ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat
     7561 ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt
     7621 attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc
     7681 taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt
     7741 ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga
     7801 aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag
     7861 tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa
     7921 tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt
     7981 tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag
     8041 atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa
     8101 atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa
     8161 accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt tttcccatcc
     8221 tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc
     8281 taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa
     8341 tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg
     8401 aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt
     8461 gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt
     8521 gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac
     8581 ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt
     8641 tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat
     8701 gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac
     8761 agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac
     8821 ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac
     8881 aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc
     8941 aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc
     9001 accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat
     9061 aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat
     9121 acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct
     9181 aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat
     9241 cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa
     9301 agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat
     9361 taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa
     9421 aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa
     9481 tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat
     9541 cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat
     9601 caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa
     9661 tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt
     9721 tttttcttct gtttttttga attaatactt gcatttataa aagacagaca attaataaaa
     9781 gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa ataaacggtc
     9841 ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat attttttcat
     9901 aaggaataaa gaatacttca tataaaatat ataaaaaaga accgcagcga gtgtcgctac
     9961 ggttctcaaa tttgttcttc atttcaacat aagtttttct tcaatttgct tataatttac
    10021 gtgcaaagaa tgaaacgact aatactaaaa tgattgcacc gatcaatgat ggaatgatag
    10081 ccattcctgc aattgttggt ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta
    10141 ctaaaccagc aatgatgttt gcgatgatcc ccattgatga tcctttattc gtgattgctc
    10201 ctgcaattgc tccaatgata ccgccgacga ttaatgccca aataaatccc attttaaatt
    10261 cctcctaact gacttcttac ttcttattgt acacgtggtt cgttattttc tttgactttt
    10321 tgtgcacctt tgcctacagc agattttgct ttatctgttt ggcgtgaagc aaattgtcct
    10381 gtggattttg ctgcttctgt tactttgtct tgtactgttt cttgatcttt ttggtattct
    10441 tcttgtgttt tgatgtctgc aacattcaca ttcacttcga taacatctaa atgtgtcata
    10501 ttgtttactt cagtactgat caattctttg atttggttgt aaatatcttc gatgtcttta
    10561 ccgtattcta cgatcacatc tagatccaca gctacttgtt tcttacctac ttctgtattg
    10621 atacctgctg ttacgttatc tgtgttgact aatttttcag ccatatttga gaaaaatccg
    10681 ccatctaatg tcaatagacc atctacatct ttcaatgcat aaccgatgat tttttggatg
    10741 actttgtctt cataagtcaa ttcaccattc atcatgcggt tttctccttt ttcatttgcc
    10801 aaaactgatc catttgttgt attcatgttt ttgttttcca tctttgtttc ctcctatgat
    10861 ttaaaaaatt gatcaactat tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt
    10921 gctgtcatga ttatgatcaa caatgtcttg aaaaatccaa tcgttaacca taaaacagct
    10981 agcagtagtc caagaccccc aaagataata gggagcttgt atgctgaaaa tagttctttc
    11041 attatttggc cctcctattc cactcgagag tgcttgtagt ctcttgtatc ttcttgttct
    11101 tcataaccgc tgtatgtgac tgccactttg accgtttctt ttgttccgag tactttagtt
    11161 acttcttctt cgatatcttg catcaataca cctgttttcc ctactaatga agaagttcgt
    11221 ttcaagtctc cgttgacatg gatatggatt cgatttttcg ttgaacgtat ccgtacttta
    11281 ggtgaatgaa tgaactcttc ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt
    11341 ttgtcgattg ttagtttgcc atcttcccgt ttcataacga aagttcctct tgactttgga
    11401 tagaaaagaa cgactagcaa gatgataata gcaagaacgg caaatactac agctgcccag
    11461 aaaatatatt gttgcatgta gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt
    11521 tcaggcataa tatacggcgc attgatcaac gcaatcgcta gaaatactga aagtagcagt
    11581 aaactcatga taacaccgat tatttttaac cccttattca ttttccacct cctataaact
    11641 tatttttacc ctgtaaataa ctctaccgac aatcacagta tagatagcga acagttagca
    11701 tagcatcagt aaataaagcg ctaccaaaca ttgctggatc ataacggcgt tttattgcca
    11761 gtctgatact gaaataacaa ttgcttactt attctctatt tacttttgat tgctggctat
    11821 acttaaagga tattattcaa gattaaaaat tgcaaagatt atgctcagtt attattacaa
    11881 gattctattt attt
//
LOCUS       DAYYCM010000082        11576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000082 DAYYCM010000000
VERSION     DAYYCM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.084"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1084
                     /locus_tag="ACHAY1_002425"
     CDS             125..1084
                     /locus_tag="ACHAY1_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8594381.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1406..1576
                     /locus_tag="ACHAY1_002426"
     CDS             1406..1576
                     /locus_tag="ACHAY1_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594382.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1590..2192
                     /locus_tag="ACHAY1_002427"
     CDS             1590..2192
                     /locus_tag="ACHAY1_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594383.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2208..2885
                     /locus_tag="ACHAY1_002428"
                     /pseudo
     CDS             2208..2885
                     /locus_tag="ACHAY1_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3196..3693
                     /locus_tag="ACHAY1_002429"
     CDS             3196..3693
                     /locus_tag="ACHAY1_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8594384.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3694..4110
                     /locus_tag="ACHAY1_002430"
     CDS             3694..4110
                     /locus_tag="ACHAY1_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8594385.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4130..5674
                     /locus_tag="ACHAY1_002431"
     CDS             4130..5674
                     /locus_tag="ACHAY1_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594386.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5817..6041
                     /locus_tag="ACHAY1_002432"
     CDS             5817..6041
                     /locus_tag="ACHAY1_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594387.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6056..6925
                     /locus_tag="ACHAY1_002433"
     CDS             6056..6925
                     /locus_tag="ACHAY1_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8594388.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6906..7640
                     /locus_tag="ACHAY1_002434"
     CDS             6906..7640
                     /locus_tag="ACHAY1_002434"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8594389.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7673..8581
                     /locus_tag="ACHAY1_002435"
     CDS             7673..8581
                     /locus_tag="ACHAY1_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8594390.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8578..9120
                     /gene="sat4"
                     /locus_tag="ACHAY1_002436"
     CDS             8578..9120
                     /gene="sat4"
                     /locus_tag="ACHAY1_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8594391.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9213..10007
                     /locus_tag="ACHAY1_002437"
                     /note="aph(3')-IIIa"
     CDS             9213..10007
                     /locus_tag="ACHAY1_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8594392.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10283..10414
                     /locus_tag="ACHAY1_002438"
                     /pseudo
     CDS             10283..10414
                     /locus_tag="ACHAY1_002438"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            10490..10983
                     /locus_tag="ACHAY1_002439"
                     /pseudo
     CDS             10490..10983
                     /locus_tag="ACHAY1_002439"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11153..>11576
                     /locus_tag="ACHAY1_002440"
     CDS             11153..>11576
                     /locus_tag="ACHAY1_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8594393.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENES"
BASE COUNT         4087 a         1816 c         2472 g         3201 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttgatat
      481 tgacacacta ccgatgagag gtaaaagaaa acccaatggc catcaggaaa aacggggaaa
      541 acaacagtat cagcgctcaa tccatgatcg atctgataat tatcctgatt tcaattctga
      601 gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg ccgtcattac
      661 tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc gtaaggcatt
      721 agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact tctttaaatc
      781 tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta accaacatga
      841 tattgatata tattttgcgg accctggaac accttctcaa cgcccattaa acgagaattc
      901 taacgggatt ctgcgtcgta atggactgcc gaaatcaatg gattttagaa aagtgaatca
      961 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
     1021 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
     1081 ttgacaaatc atactatgat acgtgtttgt ttttctgtgc tgtttagtga atgattagca
     1141 gaaatataga gagtaagatt ttaattaatt attaggggga gaaggagaga gtagcccgaa
     1201 aacttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc
     1261 agtgagagcg aagcgaacac ttgatctttt aagttgctat ctttttaagg tcaatagagt
     1321 atacttattt gtcctaatat ggttttagca gtataattga cttggtgaat aggtcattta
     1381 agttgggcat aataggagga gtaaaatgaa aaaatttatt tatcgagttt tagaaaatga
     1441 cgaagtggtg gctattttta atgagcaaca atatgcgcaa gattttatcg cttacgaaaa
     1501 gacaatttct gataagcaat ttgaaattga aaaagtagat atagctgatt ggttattgca
     1561 accgagagaa ttttagaggt tggttgaaaa tggctaaaat tggttatgca cgtgtcagta
     1621 gcaaagaaca gaacttagat agacaactga aagcgttaga gggcgtttct aaggtctttt
     1681 cagacaaatt aagcggtcaa tcggtcgaac gcccacaatt acaagctatg cttaactata
     1741 ttcgtgaagg ggatatagtt gttgttactg aattagatcg attaggacga aataataaag
     1801 aattaacaga attgatgaat caaattcaaa ttaagggggc aaccctggaa gtcttaaatt
     1861 taccctcaat gaatggtatt gaagatgaaa atttaagacg gctgattaat aatttagtga
     1921 ttgaattgta taagtaccaa gcggaatctg aacgcaaacg aattaaagaa cgccaagccc
     1981 aaggaattga aattgctaag aaaaaaggaa aattcaaagg gcgacaacct aaattcaaag
     2041 aaaatgatcc acgtttacaa cacgctttcg atttattttt gaatggttgt tccgataaag
     2101 aagttgaaga acaaactgga attaatcgcc gaacgtttag aaggtatcga tcaagataca
     2161 acgtgacagt agatcaaaga aaaaaacaat gaaaagaggg atagttaatg agtacggtta
     2221 ttttagctga aaaaccaagc caggcattag cctacgcaag tgctttaaaa caaagcacca
     2281 aaaaagacgg ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct
     2341 ttggttttgg gcatttagtc gaattagcag aaccaggtca ttataacgaa aagtggcaga
     2401 actggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
     2461 ataagggaaa gcagtttaaa attgttgctg aacttctcaa aaaggcaaat acaattattg
     2521 tcgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa
     2581 atgccttttc taaagataaa acgtataaaa gattatggat caatagctta gaaaaagatg
     2641 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
     2701 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttatata
     2761 ccttgaattt acaacaaaaa ggcgtacaag gtacattttc attaggacgt gtacaaacac
     2821 cgactttata tctcatttat caacgccagg aagcaataga aaactttaaa aaagaacctt
     2881 ttttcctgaa taattcatag ttttactaat agtctttcct aacattgatt taatagtctt
     2941 taaagtattc tgcttcatca cccattaggt gatgaaaaaa gaaccccttt ctgatttagc
     3001 ggacaaatac tttgacctaa tctcagaaga tataaaagaa aaaataataa aatatccgat
     3061 ttatactttg tataaagaac taaagtagtt agcaaatcat ttacagcaaa tcactatgtt
     3121 ccacatacca ttttacatac ttaccttatc cacgacacat gttgagatag tatcgaagat
     3181 aaagaaagtt gctccatgtt gtttttgcga gatggggaaa tcaaagccac attgacaacc
     3241 atgatgaata agcttgcttt cagtcataag ctaatcctag aaccactttt caagtcagtt
     3301 agccaaatcg atgaagaaag cgactgtgaa agaatggacg ctattgataa gctaatggag
     3361 caactattgg aagaacgcaa tacccttatt gcactgatgt ccaaaggttt cctagaacct
     3421 gctcttttta atcaggaacg aaatgtttta gatagtgaga ttaaaaatct tactaccgaa
     3481 aaaacaaacc tggtaacgaa ctctgcgagt ggggttttgc gagcaaacga gataaaggac
     3541 ctcattaatt atgtgtcagc agataatttt aatggtgact acacggaaga actatttgaa
     3601 gaatttgtag tgaacatcat tgtaaattcc agggatgagc tgacattcaa tttgaaatgc
     3661 ggtctttccc tgaaagaaaa ggtggtgaga taaatggcat atattccata tggatacaaa
     3721 attcaagatg gagtggttac tgtcgatgaa aaggcagcag gtcaagtaaa ggtattcttt
     3781 gagaaataca tatcaggact atcccttaca gtggctggcg aacaggcagg tattgaaaag
     3841 acacattctg tgatggggcg cattttgaaa aacgtcaact accttggaaa tgatacgtat
     3901 ccagcaatta ttgataaaga gatatttgat aaagctgaag aagttagaga taaacgtgca
     3961 aaggatttag gacgagtggt agagcttgcc gctttcacct ctccccctcc caaagaacga
     4021 ttcaaaatga aaaaggcaga taataagatg ccagtggatc cttttgaacg agcagaatac
     4081 ttatacagtc tgatagaaag cgaggaataa agtgacagag aaaaatataa tggttattcc
     4141 tgctcgtaaa agagtaggaa gtacagccgc aaaagaaaag ataaagaaac ttcgtgttgc
     4201 tgcctattgc cgtgtttcta cagaaacaga agaacaaaat tctagctatg aggttcaggt
     4261 ctcacactat acagagttta taaagaaaaa taatgaatgg gagtttgctg gcatctttgc
     4321 agatgatggt atctctggta cgaacactaa aaagcgtgac gaatttaatc gcatgattgc
     4381 agagtgtatg gacggtaaca tcgacatggt tattacaaaa tccatcagcc gatttgcgcg
     4441 taacacccta gactgccttc aatacattag acagctcaag gataagaaca tatcagttta
     4501 ttttgagaaa gagaacatca acaccacgga tgccaaaggt gaggttttgc tgactattat
     4561 ggcatctttg gcacagcagg aaagccagag cctttcacaa aacgttaagc ttgggctaca
     4621 gtaccgatac caacaaggaa aggtgcaggt caaccataag cgatttatgg gctacacgaa
     4681 agatgaagat ggaaatttaa ctattgtccc cgaagaagct gagattatca aacgcatcta
     4741 ccgagaatac cttgagggtc agagtctagt gggcattggt cgagcccttg aaaaggatgg
     4801 tattttaaca gcagcgggaa aaccaagatg gcgaccagaa tcagttaaga agatacttca
     4861 aaacgaaaaa tacatcggag atgcccttct gcaaaagact gtcacagtag attttctgac
     4921 caagaaacga gtgaaaaatg aagggcatgt tccccaatat tatgttgaaa atagccatga
     4981 agcgattatt cctaaagagt tatttttgca ggcgcaagaa gaaattcatc ggagaagcaa
     5041 tatctacaca ggagaaggca agaacaaaag aatttacagt agcaagtacg ctttaagtgc
     5101 catcaccttc tgtggagatt gtggcgatat ttatagacgg gtctattgga atatccacgg
     5161 aagaaaagaa tttgtctggc gatgcgtgac tagaatcgag caaggtcctg aagtttgtaa
     5221 gaaccgaacc gtaaaagaag atgaacttta tggtgcagta atgactgcga ttaataagct
     5281 acttgcaggt gggaacaata tgataaagac actggaagag aatattcatg ctgtgattgg
     5341 cgaaaccaca gaataccaaa tttcagagat taacaactca ctagaggaaa aacaaaaaga
     5401 actaatcaag ctggcgaaca agggtcaaga ctatgaccac ttagcagatg agattgatga
     5461 gctgagagac aagcgacaga tccttttagt agaagatgcc tccctcagtg gcgagaacga
     5521 gcgaatcaat gagctgattg aatttatccg caagaacaaa ttccgcacct tagaatatga
     5581 tgataagctt gtaaggaaga taatccagag cgttacagtc tatgaaggcc acttcgtcat
     5641 agccttcaaa tctggcatcg aaatggaaat atgaaaacca gaagtaaata gcccatgact
     5701 ctacagtaga gttgtgggtt ttcttttgct tgctgattat aataatattt taataactat
     5761 tgtgtttatc aaccaaatgg tttataatat tcttagcgta aacttggagg tgctgtatga
     5821 ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa tataagtgtt tccgaacttg
     5881 ctagacgtat tggccagact ccacagaatt tcaataaaaa attacaacga gaaacagtaa
     5941 ccttggatga gttgaaggcc attgctgatg tgctaggtgt caagtttgtg caggcattta
     6001 ttttaccaga tggagaagaa ataaaaatat ctaacgaata aaggaggatg tttatatggt
     6061 aggtaatgtt attcaaatag taacagagaa attatcatct ttgcctttta tagaaggcat
     6121 cgtattaggg ggctcacgtg caagaggcac ccatacagaa aattctgata tagatatcgg
     6181 aatctattac aattcagact catttgacct gacagcgatt aatcaaattg ctacagagtt
     6241 ggatgatgag aatagaaaca accttgttgt acctcccgga gcgtggggtg attgggttaa
     6301 tggcggtgga tggttagtta taaacgggtg tcatgttgac ttgatattac gtgatataaa
     6361 acgggtggaa caaataatca aagatacgga gcaaggaatt gttactgcca attatcagac
     6421 cgggcatccc catggttata taagtgctat gtatcgtgga gaattagcga ttagtaaaat
     6481 actatatgct aagaacgaaa gcttatgcga attaaaaaag caggcagaaa tttaccctac
     6541 tgctctaaag aaaagcttga tgaacttttt tatatttgaa gctgaattct ctttaatgtt
     6601 tgtaaaagca aatgcggggg cagaggataa atattatatt gcaggccatg tttttcgtat
     6661 tatttcatgc ttaaatcaag tactatttgc atgtaataat gcttattgca ttaacgaaaa
     6721 gaaagctata aaactgcttg aaacttttga atataaacct gaaaaatatg ccgaaagagt
     6781 taatcatatt tttgaagtac tcggtctttc tctttttgaa tgctacgaca tgaccgagaa
     6841 actttataaa gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     6901 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     6961 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     7021 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     7081 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     7141 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     7201 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     7261 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     7321 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     7381 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     7441 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     7501 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     7561 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     7621 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     7681 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
     7741 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
     7801 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
     7861 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
     7921 agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt
     7981 attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat
     8041 tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag
     8101 aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta
     8161 tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat
     8221 tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa
     8281 attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga
     8341 gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct
     8401 atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta
     8461 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
     8521 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
     8581 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
     8641 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     8701 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     8761 gattgccttg aatatattga caatactgat aagataatat atctttacta ccaagacgat
     8821 aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat
     8881 atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct
     8941 atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac
     9001 cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg
     9061 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
     9121 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
     9181 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
     9241 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     9301 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     9361 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     9421 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
     9481 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     9541 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     9601 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     9661 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
     9721 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     9781 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     9841 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
     9901 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     9961 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag
    10021 atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg aagtacatcc
    10081 gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga tagagttcta
    10141 tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt ataaccaggg
    10201 tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac tttttatgga
    10261 agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta ttcttattca
    10321 gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt
    10381 aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga
    10441 ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc
    10501 ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc
    10561 atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga agcatggtga
    10621 aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat ctggacagaa
    10681 aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca ataaaagggt
    10741 aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa tgatagagcc
    10801 tatatcactg gggttttatg aacagaactg gtacttaatc ggttttgcag gctaagaaat
    10861 gaatatagga attttcgtgt ggatcggatt aaaagcattt gcatagaaga agagctctgt
    10921 caaagccatg atggctcttt ggaacaaata ttaaaacaaa tgctttcata taaaaaacta
    10981 tatgccctac aatgccgttg taaaaattgg ggaaaaggga tatattgatt ttatatcttt
    11041 ctcaaaataa atataaaagc cctttaaaga gggcttttat atattaatca caaatcactt
    11101 atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattct taatgataca
    11161 atattactat acaaaaaaag aatggggcgt agttatggag aaggaaaaac taaaaatact
    11221 tgaagaatta agacgtattt taaacaataa aaatgaagca attattatct tgaataatta
    11281 ctttaaaggt ggtgttggaa agtccaaatt atcgactatg tttgcttact tgacagacaa
    11341 attgaattta aaagttttaa tgatcgataa ggacttacag gcaacattga caaaagactt
    11401 agcaaaaact tttgaggtag aattgccacg tgtcaatttt tatgaaggct tgaaaaatgg
    11461 aaacttggct tcttctatta ttcatttgac tgataattta gacttgatcc ctggcacgtt
    11521 tgatttgatg ttactgccaa aattaactcg ctcatggacg tttgaaaatg aaagta
//
LOCUS       DAYYCM010000083        11557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000083 DAYYCM010000000
VERSION     DAYYCM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.085"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHAY1_002441"
     CDS             complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHAY1_002441"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8594394.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHAY1_002442"
     CDS             complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHAY1_002442"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8594395.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHAY1_002443"
     CDS             complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHAY1_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8594396.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHAY1_002444"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHAY1_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8594397.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="ACHAY1_002445"
     CDS             complement(5395..5790)
                     /locus_tag="ACHAY1_002445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8594398.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHAY1_002446"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHAY1_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8594399.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6267..8459)
                     /locus_tag="ACHAY1_002447"
     CDS             complement(6267..8459)
                     /locus_tag="ACHAY1_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8594400.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8483..9454)
                     /locus_tag="ACHAY1_002448"
     CDS             complement(8483..9454)
                     /locus_tag="ACHAY1_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8594401.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHAY1_002449"
     CDS             10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHAY1_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8594402.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3119 a         2424 c         2119 g         3895 t
ORIGIN      
        1 ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaatt aacattgaat
       61 taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat tttgtgcctg
      121 ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag
      181 tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg
      241 ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac
      301 aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac
      361 tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg
      421 tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg
      481 tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt
      541 cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga tattcttcgt
      601 tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga
      661 caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag
      721 aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg
      781 acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga
      841 aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtaa
      901 atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta cggctggttt
      961 ctcgccttta agcagagcat atttttcgcc catgatccct tgtagttcag ggaattctcc
     1021 taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa
     1081 ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg
     1141 cattttctca tagatgctac cgattttttc atggaaagta acagttttta atttttcaac
     1201 gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg
     1261 agcagtcaat actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt
     1321 acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg
     1381 ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat atttttcttc
     1441 gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc
     1501 ttcatctaga tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtgatgat
     1561 catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt tttcttcgta
     1621 ttgttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc ttgaagctct
     1681 gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat
     1741 ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg ttactgggaa
     1801 agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg
     1861 agtgtatttc gttacataga catattccac gccttttaat tctctaaaag tgatagcatc
     1921 tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc
     1981 gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg cttgacgttc
     2041 tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt cgatcgtatc
     2101 ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct
     2161 tggtgtgact acatgagcag gtatttcttc cagcccgatt tcaagtaata agtcttttgc
     2221 catcttattc agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga
     2281 atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga
     2341 tacgggaaag ataacctgca cgctctgtta cggatactgc accgcgagca tctaataaat
     2401 tgaatgtatg gctacatttc aaaatataat catacgctgg atgaacaaga ttttcatcga
     2461 tacagcgttt agcttctttt tcaaaacgat caaaattgct tagtagcagg tcttggtcgc
     2521 ttacttcaaa agaatattta gaatgttcat attctggttg tttgaagatc tcaccatatt
     2581 tcacgccatc agcccattcc agatcataaa cactttcaac ttcttggata taagaagcaa
     2641 gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt
     2701 gctggaagta tgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac
     2761 cggcacaacc catcgatggg ttctcccagt tgtcttcaac aaagcgaata tcgtgttcta
     2821 atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata ttttcaggtg
     2881 aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat
     2941 aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc
     3001 cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag
     3061 cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt
     3121 ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa
     3181 taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg
     3241 aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac
     3301 tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa
     3361 ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttctggtttg
     3421 tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc
     3481 cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc
     3541 aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt
     3601 catttgatca atgaactttt tgctttttag atgaataccg acatattcat catacaactg
     3661 gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt
     3721 atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata
     3781 acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc
     3901 gaagcgttgc agtatttgca cttcaaagat attcgtgatg atttctgcat catctccttg
     3961 gtccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc
     4021 ttcgattgct gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc
     4081 ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc
     4141 acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg
     4201 ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agcctttcga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat tatttatgtt tcgatcttcc gctgtttttc tcaacgtacg gatttttatc
     4441 cagtttattt ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa
     4561 gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta tctctttcga
     4681 cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg
     4921 tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc
     4981 ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg gccacgtttt tgatcagcac
     5101 tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag
     5221 cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat
     5641 cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc
     5701 ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag
     5941 cagcacgttc aatagagatc atgatgtctc ctagtacacg aggtaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca
     6121 tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca
     6181 gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact
     6301 tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatt
     6361 aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca
     6421 tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga
     6481 tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca
     6541 cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt
     6601 tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata
     6661 tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt
     6721 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct
     6781 gaaggcaagt tttcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag
     6841 caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga
     6901 ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa ctgtttcaat
     6961 aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc
     7021 aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac
     7081 ccgcagacga gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc
     7141 aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt
     7201 tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc
     7261 aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7321 gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7381 ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat
     7441 tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat
     7501 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga
     7561 agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt
     7621 cctagtaatt ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg
     7681 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct
     7741 tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga
     7801 atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt
     7861 ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc
     7921 agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat cgtcgaataa
     7981 aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt ttcaaaattt
     8041 ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt
     8101 gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat
     8161 tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat
     8221 tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg
     8281 atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc
     8341 ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg
     8401 aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg
     8461 tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga
     8521 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8581 ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtccctcgag
     8641 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8701 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8761 catcatccaa tgttcgcccc cgcatgtaag ctaaaggtgc gatttcgatt acgcctcgtt
     8821 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8881 gcagataagg atccactttt tcttttagat ccccgggaag aaagcctaga ttttcacctg
     8941 cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga
     9001 ttgctaatac tactgcaaga aaagtttttc ctgtaccggc tggtcctacg ccaaatacga
     9061 tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt acccgtatag
     9121 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9181 tacctttttg ccccattttc aatgccgtca caacatcggg cgtacttacg tggattcctc
     9241 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9301 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcaa
     9361 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca
     9421 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9481 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta
     9541 aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa actagccgct tccattctct
     9601 aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa
     9661 gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa
     9721 caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa
     9781 taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga
     9841 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9901 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcgaaa
     9961 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10021 gagccctttt cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg
    10081 aatctgatat tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata
    10141 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat
    10201 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtaca
    10261 cttaggtcaa atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg
    10321 cgctcttttc ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10381 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt
    10441 aacgatcgga ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc
    10501 taaacccttt ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt
    10561 ctttttactt gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa
    10621 gtatttaaac tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca
    10681 acccatgggg aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa
    10741 tggcgtttta gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac
    10801 ttttgtccat gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc
    10861 aaaagctggt agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct
    10921 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    10981 gatcactaat ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt
    11041 aggtgtcttc actactgcta tgggactcgt tgtttctttt gcacaagatt tccataaact
    11101 gttccctaaa gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt
    11161 tgcgaatgcc ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta
    11221 tccattgtca cttgcgttaa tcttactttc cttaacagca aaattcttcc aaaagagtcc
    11281 atttgtctat caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc
    11341 aaattcgcct gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca
    11401 tcattatgtt ccttttgctg cattaggtct tggctgggtc gtcccaacgt tgcttggtta
    11461 cgctggcagc ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag
    11521 tgagttacca gaagaataac caaatcaaac acaattt
//
LOCUS       DAYYCM010000084        11411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000084 DAYYCM010000000
VERSION     DAYYCM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.086"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1123
                     /locus_tag="ACHAY1_002450"
     CDS             41..1123
                     /locus_tag="ACHAY1_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8594403.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            1457..2395
                     /gene="yidC"
                     /locus_tag="ACHAY1_002451"
     CDS             1457..2395
                     /gene="yidC"
                     /locus_tag="ACHAY1_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8594404.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2576..2980)
                     /locus_tag="ACHAY1_002452"
     CDS             complement(2576..2980)
                     /locus_tag="ACHAY1_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8594405.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2996..3805)
                     /locus_tag="ACHAY1_002453"
     CDS             complement(2996..3805)
                     /locus_tag="ACHAY1_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8594406.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3792..4682)
                     /locus_tag="ACHAY1_002454"
     CDS             complement(3792..4682)
                     /locus_tag="ACHAY1_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8594407.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4695..5174)
                     /locus_tag="ACHAY1_002455"
     CDS             complement(4695..5174)
                     /locus_tag="ACHAY1_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8594408.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5191..6957)
                     /locus_tag="ACHAY1_002456"
     CDS             complement(5191..6957)
                     /locus_tag="ACHAY1_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8594409.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(7003..7944)
                     /locus_tag="ACHAY1_002457"
     CDS             complement(7003..7944)
                     /locus_tag="ACHAY1_002457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8594410.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7941..8936)
                     /gene="lacD"
                     /locus_tag="ACHAY1_002458"
     CDS             complement(7941..8936)
                     /gene="lacD"
                     /locus_tag="ACHAY1_002458"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8594411.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8929..10122)
                     /locus_tag="ACHAY1_002459"
     CDS             complement(8929..10122)
                     /locus_tag="ACHAY1_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8594412.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10140..10868)
                     /locus_tag="ACHAY1_002460"
     CDS             complement(10140..10868)
                     /locus_tag="ACHAY1_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8594413.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3286 a         2456 c         2010 g         3659 t
ORIGIN      
        1 aaaagattaa tttaaaaaaa taatggaaga agttttttat atgacaaaaa atttaattgt
       61 tcgcggtgcc cctcaagagt atgaaataaa agaaaacgca tgggattcac tccctgaaca
      121 cctttacaaa aggcatattg acaacgtttt gattctccat ggaaaaacat cttggaaagc
      181 agtaaaagat gtttttccag atttatcaat aacaaaaatt tttgaatatt atggtggaga
      241 atgtaccgat ccacttgctg acagttttgt ttctttatgc aaaaagaagg atttcaatgg
      301 tattattgca gtcggcggcg ggaaagtagc agatctagga aaattgattg cctataagac
      361 aaacttacca atcatcatct tacctacctt agctgcgacg tgcgcagctt atactccact
      421 tagtgtggtc tataatgaag aaggagtgat gcttcgctat gatatgttcc ctaaagcaaa
      481 tgatttagtg ttgatcgatc caaagatcat tttgcactcc cctcgcgaga tgatgattgc
      541 cggtattggt gacaccttag caaaatggta tgaatctcac gcaatcatct ctcagttaac
      601 tatcaagcct atcgaagtcc aagtagcgga atttgcagcg ttacgatgcc gcgaaatcct
      661 tttagagaaa agtgcagagg caatggaagc gatggaccag caaaaaatca atcaagcttt
      721 tatcgatgtc gtggaaacaa acattttatt ggctggtatg gttggtgggt ttggcgatga
      781 ctacggccga acagctggag ctcattccat tcacgatgcg atgaccgtac tccctgatag
      841 ccatcagcaa cttcatggaa acaaagttgc atacggtatc cttgtccagc tgatgatcga
      901 aaataaatta gcagaaatcg aacacctact gccgttttat catcacttga acttaccaac
      961 gagtttagct gatatgggtt tacatttaac agaaagtgac tatcaagccg tagctgaacg
     1021 tgcatgttta cctgatgaaa tgatccactt catgaaagaa acgatcacac cagcactggt
     1081 tgtccagaaa atgaaagagt tagaacaagt gacacaggga tagttagccc cttataccat
     1141 ggcagtcagc ttttttatgg atcggctcaa tggtaaaaaa aaagaagtgg aaaaaattga
     1201 cattccactt cttttttttg cgctaaaatt atgatttcaa ggaaatatcc gttgctcatc
     1261 ttattttaga gaacggaggt gctaagtatg ctttatactg aagtattttt agctggattc
     1321 ttctttttaa cattaagcgt cttttttgat cctccaatcg gttatttcta tgaaatttct
     1381 atgaagttga ttgaattatg agcgcttttt tagtatagtt aagcttgtgt aaaaattata
     1441 aaagaggaag tattttatga ataaatggaa aaattggtta ttgggctctg ggcttgtcac
     1501 gatcatgctt ttcttgtccg gttgtgtccg catggattca aatggtaacc ctgacacttc
     1561 aggaatcatt taccgtatat tggttcaccc aatgggacaa gcgattacat atttagtgga
     1621 aaactttaat tggtcctacg gctgggctgt tatcttgatg acagtcatcg ttcgtatcat
     1681 cattttgcca ttaggtatta gtcaatcgaa aaaaacaatg atccaatctg aaaagatgca
     1741 agcattgaaa ccacaagttg aagctacgca acaaaaattg aaagctgcga caactcgaga
     1801 agaacaaatg gctgcacaag ctgaaatgca acaagtttat cgtgaaaatg gcttaagtat
     1861 gactggtggt atcggttgtt taccgcttct gatccaaatg ccgatctttt ctgcgctata
     1921 tttcactgct cgctatacag aaggcatccg tgaatcaacc ttttacggga tcgacctagg
     1981 taaccctagc ttagttttag tagcgattgc cggtattgct tatttacttc aaggctatat
     2041 ctcaactatc ggtatccctg aagaacaaaa gaaaacgatg cgtacgatgt tgatcgtatc
     2101 acccgctatg atcgtcttca tgtcgatcag tgccccagct ggtgtcactc tttactgggt
     2161 agtcggtggg atctttagtt gtctacaaac attcattacg aatgtcatca tgaaaccaag
     2221 attaaaagcg caagttgcgg aagaattaaa acaaaatccg ccaaaacaag tggtgacgcc
     2281 tcgtaaagat gtcacacctg ctgaacctgc gaagcaaaca aaaaaacaag tacaagcacc
     2341 aaaaaacaat gcgaagaatg gtcggaatgc tggtaaacag caaaagcgta aatagttatt
     2401 tgtaactagc ttcttcgatc gacccagatc atttctgatt ttatgacgat aaaagccaac
     2461 gtactgtgaa atatagaaaa ctatatttcg cggtacgttg gcttttttat acacggcctc
     2521 tttatacatc cgtatttata tccagttgta tcaccagtca agaagttgtg cttgattatt
     2581 cttcttcatc gtctcccatg ccagcaatca cttggttcac tgaaacgata ctctcacgac
     2641 ccgaaccaat aaaatctggt tctgctccaa tcatttgcga attgatgaat tcgatgacca
     2701 tcggcaaatt catgccagca tatagttcga tcgtttcacc ttccatgatt agcttggaaa
     2761 cggtatttgc aggtgtccca cccaacagat cgcaaagcac tacgtaatct ttcaatggtg
     2821 ctgtaacgga gaggaatctt tctttatacg tgtccgcgcc atcttcaggc aataaaggga
     2881 tcgtatagat attttcttgt ggtcccatga tcatttctgt actgtttttt agttcttcac
     2941 aaaatttacc atgactaatc aagactagtt gtctactcat ctttccaatt ctcccttatt
     3001 tcgaaatcac acctaatgca gataatgcta cacatacgat caatacgatg aatattgctc
     3061 gagtaggtgt catatttttc ttacctaaca tccaataaac gccaccaacg attccagctg
     3121 gtaaaagttt aggtaagatc atatcgatat agttttgcat attcaacgtt acatccccaa
     3181 tcgttggtgc ccatgagaat ttaacattga tcatggttgc tactaacgct ccaaccatga
     3241 aaactcctag taaagtagcc gcatctgtca atgcagtcaa gcgtgattgc atcgtggtaa
     3301 ctagagaaat cccttctttg taggcaaatt ctagttgttt ccaacgaaag accatgacag
     3361 cgacctgagc aaggatccag ataaggatac ctactggatt accatctgtt gccatatttg
     3421 cggctaaagc tccaaatatg gtcgggatca atgccgcaaa tacagaatca ccaattgcag
     3481 cgaaggaacc catcaatcct gctttcatcc cagaaacagt ttcctttgaa gagactcctt
     3541 ctttttcttc gatagcaaga tcgatcccag taatgatcgt gttaaagaaa ttagacgtat
     3601 taaaaaattg cgtatgtgtt ttcatcattt cttttaattc tggtgtatca tcaccataga
     3661 tttttctaag ttgtggcaag atagtataca gataacctga accttgcatt ctttcatagt
     3721 tccaacctaa ttggaaagta aacaaacttc tacgattgat ctgcttaaaa tcttctttgg
     3781 tcaatttata attagatttc gtcatcttcg atttccccct catcagattg tgattgctga
     3841 cttactactg gatgttggtc attcttttga tcattcttat aactgatcgt tgccagtgct
     3901 agaccaatca atgccacagc aagcatcggt aatgaattga atgttcctgg gaaatctttg
     3961 accacgctag ccacacttgt tcctaacaat tggacattcg agaacactgt tgctaataac
     4021 gctgtgatcg taaatcctaa aatcagataa gaaatgtgtt tctttactgg taggtagcgt
     4081 agtaaaattg caaacccaac tgctggtaag actgcccctg caacagataa gccatctcct
     4141 agccatttta ggtcgccgtt taaaacagcc acaacatttt ggactaagcc accaccgaat
     4201 gctagggcca agaatacagg aaccattctg gaaagtgccc aaggtaatgc ccccatcaag
     4261 aagttccgtt caattgcttt gtaattcatt gtttccacat gttgatcgat ccgatgtgca
     4321 aaatacgtat tcgcaaaacg tgccaaaata tctaattgga tcatcaaact tgctacagga
     4381 accgcgatgg ccgcaatcgc ttgttctggg ttcatcccta gagaaatgga gaaagctgtt
     4441 gctaagatcg ttccagagtt ggcatcgatt tttgatgcac caccaaatgt ccctacccct
     4501 aaaacagtca actgcatact tccaccaatg attaaacctg cttttaaatc acccattact
     4561 aacccagcat ataatcctgc aaaaactggg gcgctcattg aagaataaat ctgtaattca
     4621 tctaaaattt gataccctgc atataatgtt aataataata tctgccacca ttgaatatcc
     4681 atgaatcatt cctcctattt gattaaaaga ctcataaagt cttctgcttt gtcatttggc
     4741 accatttgag cgatcaaatg aacgccaagc tgatctaatt tcttgaagac ctctacatcc
     4801 gcatctacga cattgattga ttttgtgatg gaacgtgttt catctgtttg tgacatattg
     4861 ccgacattta attctttgat cggtacacct ttttctacta gcgctaagat acgatctggt
     4921 tttttcgcta cgatcagcac tcgttgagaa tcatatttac ctgctaaaat atttgcggct
     4981 gctttttcaa taggtaaaat acttaatttg actccagctg gcgttgccaa ctttaaccca
     5041 cttttttcga tcgcatttgt tgctacctca tcgtctacga ccatgatccg accaatattc
     5101 aactttgtcg tccaaagatt tgccacttgt ccatgtatca aacgtccatc aattcttact
     5161 ccaataattg ccatcatcca ttctcctttt ctaacttttt tctattggtt ctttgacaaa
     5221 atctataact tcactgaatc taccttctgt ttcaaagata acgatttcat tttgtccttt
     5281 tctaaataaa ccctcgggaa caaacagtga aagaattggt cctacttccc aaaaacgacc
     5341 aatattcgtg ccattcaccc aaacgatccc tttcccaaac tcactaaggt ccaaataagt
     5401 atcaccgatt tctgacagtt ccgcttcaaa gcggtaaaat gctggttgac ctgcttccca
     5461 tttttttgaa aaatcgatgt gttctgtagt ctctaaaggt aaacaatact gtgtccattc
     5521 tgtgataaaa tgaagatcgc tcatcactcc tgtacgtatt ccttttcttt gcgtatcagc
     5581 tagcagcttg tgtccatagt tgacccgtcc catattttca aataatagat caatttgatg
     5641 gtcttctttc tcaagtgtca atggaatggt ctcaccgatc tctgtttggt attgaatcgc
     5701 ttgcttttct tcatcgagaa aaacatggac ccgatcacgt ccgtcgatga tccgtaattt
     5761 ttcattgacc gcatcttttt tgatcgttgt tcgatacaat aggtacccag tattctgacc
     5821 caattcttcc attgtttgag gataggtcga gtggatcggc ttgctgatgg tatccagtgt
     5881 tgcgaaaaag ctgacttttt ctttcaagtt gattcctttt ttgacgattg tttcttttat
     5941 ccatggttcc atttgctgca tttctggaaa attttctgcc atcattcgtt tcagagcaaa
     6001 atatttttcc gttggatttc cctgctcatc caatggtgca tcgtaatcat aggaagtgac
     6061 ttgcggcaaa tcaatcgcgc ctctggctga acagccattc ataaacccaa aattggttcc
     6121 gccatggaac atatagagat tgatactgcc gatctgtaat gcttctttga ctgcatccgc
     6181 taattcttcc ggatctcttt taatgatcgg ctctttccaa cgattaaacc agccatccca
     6241 aaattccata cacatcaacg gccatttttt accatgtgct tcaaaaaact gggccatatc
     6301 tgcaaaattt tcttttgctt ttgagccaaa attccctgtc gtaaaaacat catcgtcaat
     6361 caaaatgcct gctcttaatg tggcttgcca aggtccatca gaagtgaata gcggtacatc
     6421 gattccacct tcctccaagt atttttttac ttggcgcagg tatgctttct cttcaccata
     6481 cgaaccgtac tcattttcta cctgcaccat taagacgttg ccaccatgag tgactagatg
     6541 aggaacgatt ttaggtagta gcgtttggta ataccgtttg acatacttca tgaagcgagg
     6601 atccgctgaa cgaatacggc actcctttgt caatagccaa gcaggcaagc caccaaattc
     6661 ccattctgca caaatataag gggctggacg tacgattgcg tataatccaa gggattgcgc
     6721 taactctaga aaatgggaaa gatccaatat tttcgaaaaa tcataggttc cttcttcttt
     6781 ttcatggaga ttccaaggca cataggtttc cacagtatta aatccaagcg ctttcaaatt
     6841 gtgcaatgac cgctcccaat tcgtcggatg cactcggaaa taatggatgg caccagataa
     6901 aattttgaaa ggttgaccgt ctaacaaaaa ctcttctttt atttcaaatc tagtcaagca
     6961 aatttcctcc tcgtttttag aaaccgcttt ctaattttat aatcaacgaa tctttctgac
     7021 gttgatttcc cccctaaaat aatcgacttt cacaggatcg atccatcctg ttttttcttg
     7081 ttgtgcattc gccatcccac aggccatcgc aaacgcaagt gtttcttcaa tcgtatagct
     7141 ctttgataga gcatgtgcta agcctgcaat agtcgaatct cctgacccca caggattcac
     7201 caccgcgatt ttaggaatcg taacctgata gaagtgtctc cctactttag cgattgctcc
     7261 ttttgcgcct agcgaaagaa cgatccaatt gattccttca aaaagcggat gatccaataa
     7321 tgattggatt tcatctaacg aagtagccga agaaattgtt tggtggacca actctcctaa
     7381 ttcttccaaa ttaggtttga tcaaaaatgg tttttccttg ccttccaaga cttcaagcaa
     7441 ggtttgacca gaagtatcta acaatactcg tgttcctttt ttattcgtta gggcaagtaa
     7501 ctctgcataa taacttttag aaaatccttg aggcagacta cccgaaatcg taaagatatc
     7561 agttgtttcc attagtcgtg caaaatgctc ggtaaattta tgttgctctt cttccgtcac
     7621 aacaggaccg ctttccaata gttctgtttg attcactcca tgtaaaatag caatcgaatc
     7681 tctcgtttcc cctttgatag gtaaaaattg atgcgttatc cctgattgat cgagttgttc
     7741 tttgatatat gctccatgct gcccgccgac taatccagta gctaaaacat catctgctaa
     7801 taaatgaagg acacgagcaa cattcaagcc ttttcctcca gccgttttgt tgaccgtttt
     7861 gacccgattg acttggtcga tccgtaattc atccaacgtg taagcgatgt caattgaagg
     7921 attcatggta actgtcgtga tcattttgct gtcaccgaat cttcttttac tttgtcaaac
     7981 caaggggtgg cgttttcatt tatcacttta ttcaacgatt cgatattttg tcgcccttgt
     8041 gttctcaacc actcaatcgc accttctcta ccattttcaa catatgcttt gactccatct
     8101 ttccaagtcg cacgtccgca taacacaccg ttgaatgttg aaccagattc atgagcaaag
     8161 atcaatgttt gttggaacaa tgcagtcgaa acccctgcac tcaagaagat aaatggtaac
     8221 ggtgttgcat cactttgttc cttgaagaat gccgctgctt cttttttact atacgcaatt
     8281 tcaccatcag aataaccttc tacatagttc atattgactg gcacttctac tttcaataca
     8341 tccactttat atttaggatc agaaaattct ttcatcatct cgatcacttt atgcggtttg
     8401 atcttcgcat attctaatga agtgctgtca ttggttttgg catcatacga aaccaattct
     8461 agataaaacg gacgcttttc acctgcacat tcactgccta aacgttcgat aaatacatgt
     8521 ttcaaatgat tgatctctgg gtcttcatct acatcataat aaagcaaaaa cttgatcgca
     8581 tcggctgttt gttcctttaa tctaagcgta gaccaatccg ctaataaatc aggcagacgt
     8641 cctggagttg tcgcatcata acccgttttt tcataggcta aaagtagtcc tgcttcctga
     8701 tgtctggctt tagcagctgg caatccatac tctggatcta gtaaaatagc agaagcataa
     8761 ggcgttaatt ctgctgatac taattctttg aattcagtga tctgttgcgc cgttggttct
     8821 tttcctaacg ctttgatcat ctttttcaaa gcaccgcgtt gatcaattgc caatgcactg
     8881 atgattcctt cttttgttga taaactcttc atagctgcaa ttttttcttc attcatcctt
     8941 cgtttcccct ttgttcacat attcatggat aatgacacct ttgaccactc gattgactgt
     9001 acctgtaggc gatggtgtat ctggtttatt cgctactttt atcgacgtat cgagcgcaat
     9061 gatttgggct gtcatgatca taggtaaggc aagatacgca tcaggtaggc tgatcgaagc
     9121 ttctaacaaa agacttgtac ctgtgaaatc gatgtttcct ggttgggcga tcgcgatgac
     9181 actcgtggta atttggtcat cttttatttc ttctagaata tctaagtcat aatttcttgt
     9241 atacggttgg ttattaacaa aaccaataac aagtgtttcg ctatttaaaa acgattttgg
     9301 tccatggcgg aagcccatag aagaatcaaa gatcgttgcc actttaccgg ctgtcaattc
     9361 caatattttc aattgtgctt ctctcgttgc accagccaaa cttcctgaac caagataggc
     9421 gatacgttca aatgacttag gcaataattt ttggatttct tgatctcgtg aaattagatc
     9481 ttctccagtt ctgatcaatt gttgaagata actttctttt ttctcaacgg cagtttggtc
     9541 gaatacccat aacgcactca acaacatgca cgtaaaactt cctgtcatgg caaatcctgc
     9601 atcatttgat ttttctggca tcagtaaaag ataatttcgt tcatctgtcg ctgcattctt
     9661 tgctaactga ccttctttag cacaagtaat cgtcaaatga aacaatgtct tgatttgttc
     9721 attggcaatc ttgactgttg ccacactttc tggactattt ccactacgtg caaatgacac
     9781 cagtaatgtc ggttcatccg gaaatagaaa ttcttttggt gaagacacga tatctgtcgt
     9841 accaaagcta tgaaaaataa agtgatcgtt atcaccgtat tcatttaaat aaggtactgc
     9901 tgtatccccg acatattgtg atgtgcctgc accagtaaaa atcactcgaa ctcttttgcc
     9961 atttgctgct ttttttatct ggtcaaaaaa caatgctaat ttttcttgat tctgtgctaa
    10021 tatctcagct gtttctttcc ataattgcgg ttgctgtttg atttctcgcg tagttatttc
    10081 cgcacccaac tcttttaatt tatccttcga tagttcaaac atgttgtttt ccctctcaat
    10141 cattttcgga cgtggcggat cttataatga aactggtctg ctctcgctac actatccgta
    10201 tattcaataa tttcattttt cacattatag gtcgttcggc ttagatataa taccggggca
    10261 ccatcagcga tacgaagcgg atcagcatct ttcttttgaa cgatacttgc ataaaattct
    10321 tcatctgcta attggacttg ctgttcataa tcctgagaaa acacatcata caatggctta
    10381 ctttcaagta atggcagatc aagaccaata aatagttttg caggtaaata agaacgttcc
    10441 accatcaatg gttcgtcatc cgctaaacga agtcttttca atttgaaaac tgtttcgcct
    10501 aaattgatgt ttagattctt ggcaaccttt ttgtttgctt ccatttgctt gaattccaag
    10561 atgactgtat taggcacccg tcccaattcc ctcatctctt ctgtaaaact atacgctccc
    10621 gccagatcag acgacttatc tgatagatca gacacatatg ttccttttcc gtgtcggcga
    10681 taaatatacc cttgcgtctc taattcttgc aaagcaaggc gaaccgttgt ccgactcact
    10741 ccataatgct gacaaagttc tctttcagat aacaatttgt catgaggtgt catctgtgtt
    10801 tcgatacgtt ctttcaatac gtcaactagt tgataatata atgattttcc tttacttgag
    10861 ctctccataa aaatacctct tctttactgg ttataaccac aacacaagaa tacccacata
    10921 ttcctcattt gtcaaagggt ttttggcgta aaaaagctat tataacaagg tttcaaggta
    10981 atttagaact ggtatcaaaa gatacctcaa aaataccagt tgacaaataa aaaacaaaaa
    11041 aaagctgaat cttggcaaag attcagctaa atggtctaga gaagtaaaga aaaaatattc
    11101 tttatacatc tgtggctatt tctactatcg acaagcaaat gggcctaata gactactatg
    11161 taaataaaaa tggctttctc taaataaagt aggatttttt attttttaga taacttttct
    11221 ctcatgccgg tctgatagtc gaaattttgt tgtacatttt taaaaataac aaaaattttt
    11281 tatgataatt ctgtcttttt ctagtttcct tatggcgtaa gtttaacgaa taagaattta
    11341 gtatctatac ttttatatta caaaaaatat tcgtaaaatt cgggctctat acttttcgat
    11401 gtttgtgaac t
//
LOCUS       DAYYCM010000085        11205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000085 DAYYCM010000000
VERSION     DAYYCM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.087"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1133)
                     /locus_tag="ACHAY1_002461"
     CDS             complement(90..1133)
                     /locus_tag="ACHAY1_002461"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8594414.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(1123..1614)
                     /gene="coaD"
                     /locus_tag="ACHAY1_002462"
     CDS             complement(1123..1614)
                     /gene="coaD"
                     /locus_tag="ACHAY1_002462"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8594415.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1611..2171)
                     /gene="rsmD"
                     /locus_tag="ACHAY1_002463"
     CDS             complement(1611..2171)
                     /gene="rsmD"
                     /locus_tag="ACHAY1_002463"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8594416.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2290..2628)
                     /locus_tag="ACHAY1_002464"
     CDS             complement(2290..2628)
                     /locus_tag="ACHAY1_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8594417.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2612..3058)
                     /locus_tag="ACHAY1_002465"
     CDS             complement(2612..3058)
                     /locus_tag="ACHAY1_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8594418.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(3039..4163)
                     /locus_tag="ACHAY1_002466"
     CDS             complement(3039..4163)
                     /locus_tag="ACHAY1_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8594419.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4184..7612)
                     /locus_tag="ACHAY1_002467"
     CDS             complement(4184..7612)
                     /locus_tag="ACHAY1_002467"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8594420.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7638..8801)
                     /locus_tag="ACHAY1_002468"
     CDS             complement(7638..8801)
                     /locus_tag="ACHAY1_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8594421.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8804..9106)
                     /locus_tag="ACHAY1_002469"
     CDS             complement(8804..9106)
                     /locus_tag="ACHAY1_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8594422.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9220..10992)
                     /gene="recQ"
                     /locus_tag="ACHAY1_002470"
     CDS             complement(9220..10992)
                     /gene="recQ"
                     /locus_tag="ACHAY1_002470"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8594423.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3060 a         2446 c         1915 g         3784 t
ORIGIN      
        1 tttgttttta tttttatcgt gaagattttt gacgaaaaag aggggttgtg acagaactcg
       61 tgtcacaacc cctctttaat tatttagttt catttcattt tctctagata gtcaagcgct
      121 tcttgtactg tttttactgg tacgattttc attgaggaat tcaatttttt agcggcagct
      181 tttgcttctt cgtagttggt ttttatttca ggatattctt tcttaacatc gtctgaaatc
      241 gtatcatctg gtgcaaagaa aatctctacg ccagctttat ccgcacttgc cactttttta
      301 tcgattcctc cgattcggcc aacttctcct tcactgttga tcgttccagt tcctgcaatt
      361 tgcttaccat gtcgcaagtt tttaccagtg acttgttcat agatttctaa agtgaacatc
      421 aatcccgctg atggcccacc gatagagcca gaattaatac gaacatctgt atctgattcg
      481 atctcggtat ggtcagtaag cgtgatacct attcctgcct ttttatctgt tggtaactcg
      541 atcagatcac cagaagctgt tttttcttca ccattatgca aataactgat ttcgattgtt
      601 tgtccaactt tttggctttt tacataatcc ataaatgctt ctgtactttg aaagcttttc
      661 ccgtctactt ttgttactgt atctccaact gaaagcttgc caaagaaatt cgatgttttc
      721 tctacactca tgacatatac gcctttgaat tccattttat aaggcttgtc tgcaagtttt
      781 aacgcttgtt cgattgctgc attttgggaa gaatccatat aatactgttg catttgatta
      841 tattcttcat ttgtcgcccc accagtcaat tcttcttcac tgatcaattc ttcaaaaggg
      901 gtcattttag cttttaaagc taagaaacct gtagcttgcc tgataccaac agaggtaaga
      961 gagaaagacc cagaaccttt atcttcctta tcattgaccg taattagttc tttcagatcg
     1021 atcgtggctc ccggtgattc tacataatac ggcaagggaa ctactacaaa tccaatgatc
     1081 aacattccaa atatgataat cagtactgtt ttcaaccatt gtttagcttt catttgattc
     1141 acgctttcca gctaaagctt cgttgatcac tggcggtaag tagctgctca catctccgcc
     1201 aaagtgcagt acttctttta aaatactgga actcacgtga ctatattctg gttttgctaa
     1261 taagaagaca gtttccaatt catcatatag atgatgattc atctgagcaa tctctcgttc
     1321 atattcaaag tctttgatac ttcgtatgcc tctgatcaac gcatctgcac caatctcttt
     1381 tgctgtttct accgtcaatt gattttcttg atgcatgact tttacattag gaagatgaga
     1441 aacagctttt gtgatcaatg tcattctttc ttctggggga aacaaacttt tctttgaagt
     1501 attgataaaa acaccaatta ctacctcatc gaataattta gccgcacgtt ccaccgtatc
     1561 taagtgacct tttgtaaaag gatcaaaact tcctgggaat aatgcacgtt tcattgattt
     1621 ccctcttttc tatagatagt tacggccgtt atgccataaa tctgttttct tatttgatgg
     1681 aatccagcca cagtttctgg caattcgact gttttatccg tttcgcatac gataactgcg
     1741 gttttagaaa aaagtcccag ttcttccatt ttatggattt gatcgattat tttttgtttt
     1801 gcatatggcg gatcaagaaa caagtagtcg aattgttgtt tctccgctgc tagccgttcg
     1861 attgctttgt ccgcatcaag ttttattgtg gtaaatttct ctggttcttt cgtaatttcg
     1921 atgttttctt tgatgatttt tagcgcttgg taatttttgt ctacgcatac cgcatgactg
     1981 cagcctctag atactgcttc gatagctaaa ccaccgcttc ctgcaaacag atccaatact
     2041 gtctctccat caaaatatgg tccaatcata ttgaatatgg attcttttac tttatctgta
     2101 gttggtctgg tgttcgcccc tgctaaactt tttaaacggc gcccgccata atttcctgat
     2161 attatccgca tctactcact ccttagaaat ggtactggta ctagtatacc aaaaaaatac
     2221 ttggacaatg atcaaaaatc gatcgttatc caagttattt tttatttaaa aactgctcat
     2281 aagagatttt taaagatgtt cagctaatcg gatttctgta tttagctctt caatttcgaa
     2341 accttcgtct tttaatactg ttttgtttcc tattttttcg gcaaaattca tttcgatatc
     2401 cggtcggtaa gaacgttcta cgtttcgaac aaaatgtaat ttacggattc gttcttctgt
     2461 ttcttccaat gtctcttcat ttaagtaaag cacgacatat ttcattcttc ttgagacata
     2521 atgcaacaag ccgaactttt tcagattctt taattgtttt aagctgtaga cccatacgat
     2581 cagcccgcgt cgttttgtta tttcaaattc actattgtct aattgcatga caattacctc
     2641 cgcatccaaa gtgttttttc gtttcaaaaa acgggctacc tgcatctact ttgatttcat
     2701 tagaaacttc ttcggcaagt acttggacaa tttgatccaa cagactttgc agctgtgttt
     2761 cactatatcg gaaagctgca acatgctcat ccatatcaag ctgtcttttt gcttttcgaa
     2821 cagctcgttt tttttcacga taatcaggtg catactcacc atatgccgct acattttcaa
     2881 atgcttcttt cttagacaag aacgcttctt tcaactggca aacttttgca gattcttcca
     2941 ttgactgttt atgttcaaga taagaagcca tcgttttact cttttttatc atttcagcga
     3001 cttggaggca ttgatcctcc agttgaaaca gctcttcgtt aacgatcact gctatcacta
     3061 ctttctccgt tttcttcttg caataagact aacatatttt ctttagaaaa agcaatgcct
     3121 acagaatccg ttcccgggtc catgaaacgg tcatggttgt aaggatcgct gtaccaatat
     3181 aaaaacgaaa aagtagcgtc aatcgccgga tgaataaata gcccactagt attttctaat
     3241 ttttgtttac tgattaactt ctccaattca tcattcgtca gtgagaattt cactgtcgtt
     3301 tgagcactgc ctagcgattt ttgccaatca gccatacgtt cctctgacaa tatttcctca
     3361 ggccttgtca aaaaatcatg tagtagcagc tgactgtggt tttgaaaggc aagtgtttga
     3421 ttgtatatgg gaagttcgtc ttctcttcta agcatattta ataaagtcgt actttttcgt
     3481 tctttaacgc gattgattga ttcccaatca gccgatttat ctgcttgata atgtggaagg
     3541 ccttcaccaa acacttgata tggcaatagt ttcaaaagac tatctttatc taaatacaca
     3601 actgaaaata ggccgtttgt tgtttgatca aaaaataaaa tagcaaaagt gtcgttatca
     3661 aaagcaatta gcggacgata attcatatcc tcttccatta gttcgattcc aacttctgta
     3721 tcatggtact caaaagtgaa attagtatag atagtagtga ttttcgtaag atcttgcatc
     3781 gtcatcccta aataaaacgg cgcgatatca gcaggccctg tttgcagtac tttaacagtt
     3841 gtgactctgc tgtcttggat gttcgcttca aatgtgagat gactagcttt attttcataa
     3901 tatcttgttt caaaaccaaa tccgctagtt aatcgatcat aggcttggcc aaaatccttt
     3961 tccagctgat cgatcggttt tccgatataa gtagaaaaac cagttgcttt aagctcctgg
     4021 tatttccacg ttgtcatttg gtttgatatt tgttgatcag agttttcctt cacttcaagt
     4081 tttggcggaa acaagactgg ttccatgtag aatgccacaa taactaatag cagaatcagc
     4141 caaaacaaac ccagtctttt catgtttccc cacctttcgc ttattatttc tcagtcactt
     4201 caatcaaaag gtcaccagac gaaatagtgt ctcctgaaga cacatataca tgtgcgactt
     4261 ctccgtcaaa acgtgcttcg atggccgttt ccattttcat cgcttcagta atcatcaacg
     4321 tatcaccttt tttaacgtta tcgccttttt tcactaatac atccaaaaca gaaccagaca
     4381 tcgttgctcc aatttgttct cggtttgtcg gttcagcttt caatcgggtt tggaccgtac
     4441 tgatgatcga attatctttg atagtgattt cacgtctttg tccattcaaa ttgaagaaca
     4501 gtacgcgatt cccttcgata tcgggttctc cgatttcatc taaacgaatg ataagcgttt
     4561 ttccgcgttc gatttgtaca ttgattgttt cacccaaacg tataccttgg aaaaatgttg
     4621 gtgtgtccag taatgtaaca tcgccaaatt gattatatgc tttttgatag tccaaaaaga
     4681 cttgcggata catcaaatag cttaatactt cgtcttgttt tggttcatag ccaatttttt
     4741 ctgccaattc tttttttact tcgttgaagt cgactgattc agccaaaaga cctggacgtt
     4801 cattcaatgc aggtctgcct ttaagaataa tcttttgcaa ttttttagga aatccgccaa
     4861 cgggttgccc tagttctcct tggaagaaag tcacaacaga ttcagggaaa cttagtgttt
     4921 cgcctttttc ataaatatct tcttctgtaa gatcattttg gaccataaat agtgccatat
     4981 ctccaactac tttagatgat ggagttactt taacgatatc gccaaacatc aagttgacag
     5041 tatgatacat ttgtttgatt tcatcccagc gatgacctaa tccgacagct tttgcctgtt
     5101 gctgtagatt cgaatactgt cctcctggca tttcatgcat atatacttct gtttcaggag
     5161 cattcaagcc gttttcaaac ggtttgtaat acatgcgcac atcttcccaa taatggttta
     5221 atttctgtgc attttcaatc gtgatttctg gcagccgtgg accattgacc agtgcataat
     5281 acaagctgct catgctaggc tgactagtat tcccactcat tgcactcatt gccacgtcaa
     5341 cgatgtctac tcctgctttt gaagcagcag aataagtgat gattccattc ccgctggtat
     5401 catgagtatg cagatggatt ggcagatctg tcgtttcttt caattcacta atcaaacgat
     5461 aagctgcctg cggtttaagc agcccagcca tatctttgat tgcaatgata tgcgcaccca
     5521 gttgttctag ttcctttgcc atgtctttat aatactgaac attgtatttt gcccgacttg
     5581 gatcgttgat atcgccagta tagcaaatag ctgcctctgc gattttacca gtatcccgta
     5641 cagcttggat acttttttcc atttgtggag tccagttcaa actatcaaag attcgaaata
     5701 catcgattcc ttgcgctgct gcttctttga caaattccac caatacattg tcaggataat
     5761 tagaataccc aacggcattt gaacctctga aaagcatttg cagcaacgta ttaggcatta
     5821 aagaacgaat ttttctcaac cgttgccaag gatcttcatt caaaaagcgg taagccacat
     5881 caaatgttgc tcccccccac atctcacttg aaaagagttc aggaagacct tctccagtca
     5941 aacgtgcaat ctgcttgaaa tcctttgtcc gaacacgagt tgctagcaga ctttgatggg
     6001 cgtctctaaa tgtcgtatcc gtcaacaaca aattttcctg tcctttgatc cattcgacca
     6061 aagcatcggc acccttcgca tctaatacat tcttagccgt tacgtaatca gttcttgtga
     6121 ttagatcttt aggcatacgt ggttcttcat aaaatggttt ttcgccactt tcgatacctg
     6181 gaaaaccatt cacagtaatt tcaccgatat atttcatcgt tttgtttccg cggtctcgta
     6241 gtcgaggaaa ttcaaataat gttgctgtac tatcaatgaa ggttgttttt gcatttcctg
     6301 attggaattc aggatgtgtg atgacattga gcataaatgg aatgtttgtt ttcactccac
     6361 ggattctaaa ttctttcaag caacgttcca ttttctgaat ggctgtttca aaggtagcac
     6421 catgcgtaca tacttttact agcaatgaat caaaatacgg tgtcactaca tagcccgcgt
     6481 aagcatttcc gacatccaga cgtacgccaa aaccaccagg tgaacgatac gtatcgattt
     6541 tccctgtgtc tggtaaaaag ttattcaacg ggtcttctgt agtgattcgg cattgaatgg
     6601 ctgaaccatt taatttaatt ccttcttgct gaggcaatcc gatttcttta tgcagatcca
     6661 aaccttgagc aatcaacagt tgcgttgtta cgatatcaat atctgtaatc atttctgtga
     6721 tcgtatgttc cacttggacg cgagggttga cttcgataaa gtaaaaatca tctccttcaa
     6781 caagaaattc tactgtacct gcgttgacat acccgacata tttcatcaat tggacagctg
     6841 cctggcagat tttttgacgt tgctgttcat tcatagagac acaaggcgct acttctacta
     6901 ctttttggtg acggcgctga acagaacaat ctctttcaaa aagatggatg acgtttccat
     6961 gcgtatcccc gagaatctgt acttcgatat gcttaggatt agcgatgtat ttttcaacat
     7021 acacttcatc actaccaaag gctgcttttg cttcgctttt cgcacgttcg tagccctctc
     7081 ttgcactctt ttcatcgtga gctacacgca tgccgcgtcc gccgccgcct agagctgcct
     7141 tgatcataat cggataaccg tattgtttcg caaactccaa ggcatcgtct atagaagcga
     7201 ttggtccatc tgtgcctgga atcgaagcaa tccccgcttc gatagctgca gcttttgctt
     7261 tgattttatc tccaaaaata tctaaatgat gcagtttggg tccaacaaaa atgatgcctt
     7321 cttcttcgca ccgttgcgca aaattcaagt tttccgacaa aagaccgtaa cccggatgaa
     7381 tagcgtcagc tccgcattct ttcgcgatag aaatgatccc ttcaatatct agatacgcat
     7441 caatcggttt ttttccttgt cctactaaat atgcttcatc cgctttgaaa cggtgtacgg
     7501 aatattcatc ttcttctgca tagattccaa cagttttaat tcctaattct gtacaagcac
     7561 gaaatacacg tacagcgatt tcacccctgt tagcaactaa aacttttttc atcgaaccac
     7621 tcctacatct ttttttatta taaacctaag ctcttgcgtt tttcatccgc actgatgttg
     7681 aggacaaagc cgacacaaat cgaaagcatc agcaaactgg agcctccttg acttaaaaat
     7741 gggaacgtga tccctgtcaa aggaataacg ccagtaattc ctccaagatt gacaaatact
     7801 tggatcaaaa acattgcgcc gatcccaatg cacaaaagcg aattgaacgg atcttttgaa
     7861 cggataccta caaggacaat gcgcacaatc ataaagaata gcaacgctaa tatcaaaatc
     7921 cccataatca ctcccagttc ttccactacg attgcataaa taaaatcggt ttgtgcttct
     7981 tgtaaaaatc cttttttctg gatactgtta cctaatcccc tgccaaatag tcctccgttg
     8041 aacatggcat agtagccgtt gactagttga tgccctttat ttaattcgtc tgagaaagga
     8101 ttttggtaaa tagcaaatcg attatagata tactgtaaac gtccgggtaa gatctttccg
     8161 tttgtgatat tgatcagcca gatcgtaaaa gtacttagac aaaagcctcc tactcctaca
     8221 atcaaagtat acacataatt gactccgctg gctaaaagca gaaccaaaat aatcaaaaag
     8281 ataactgccg cattaccaaa atcgggaaga atagcgacta atgcagtaag tccgataacc
     8341 attgcaagtg gacgtttcac agtcccaaga aaatcttttt gtacactgtt ttgtctttta
     8401 gataaagtca gagacaggta ccagatagaa atgattttta ggtattcggc aggttggatc
     8461 gagaatcctg caatttgcag ccaacctttc gccccattga tctccttacc aaaaaacaca
     8521 acaatcagca gcaaaatcgt caaaactgcg atggctgcca tgatcaaacg ctggtttttc
     8581 aaaacatctg ttttcatttt ataaagtagt gcgatagcaa tcaagctaag cacccaaaaa
     8641 atactctggt taatgactga tgcggacgga ttttgtccgt tttctaataa tagatacgac
     8701 gtagaactat ataccatgat caatcccaat acacataaaa ttaaataggg aatcaagatg
     8761 ctatagtcta atagatgcct ttttttcact ttcttaggca agtctattcc tccttgccaa
     8821 cttctttttt atcataaatt tcttggtaaa cctcactata taactgattc aattctcgtt
     8881 ctagctcact taacatcagc tggccctcgt tctttccgat tactcctata cggacagcaa
     8941 aaccgacttg ccgggaaaag ccgtacatct gtgtatctac cacttcttcg aaggctttgc
     9001 attgtgagat acagagactg ttgttttgat ttcggattaa tcgtttgatc cgttctgctt
     9061 catcatttaa tacttggatc gcaagttttt tcgacaaaga atccatctgt gcttacctcc
     9121 cattctaatt ccttaattat aacatatttg attttcataa ggagcaaaaa aaacagattt
     9181 aaccagttgt ttttattcat ttttacgtat tcgttctttc tacttatcca gaacgttaga
     9241 gtccgcattt tttatccgag ataaaaaggc ttcgccatat ttatcaagct tgctttggcc
     9301 aactcctttt atctgaagca tctccaatct gcttgatgga cgtattcggc tcatttcttt
     9361 caatgtggaa tctgaaaata cgacatacgg gggaacgcct gcttctgatg ctagatccgt
     9421 tcgcagttcc cgcagcactt caaatagtgt atcggtttct tcatccgata gctgctggac
     9481 tttcttgggt tcctttcgat aaacagattc ttgtcctttc aatacttgta tccctctttc
     9541 tgttactttc aaaacaggat actcaccatt gatggtcaga agatagccat tcgatatcaa
     9601 atattcaatt aactgcatcg tttctttttg cgattggttt ttcatcagtc cataggtaga
     9661 taaatgtcca aaacctaaag cttgcagctt ttgttctttc gatcctgcta agactttcat
     9721 cagcatttgt ttaccatagt tctcacccat acgttttaag caagaaagaa ccatctgtgc
     9781 ttctgtcgtt acttctacta tttctctgtc atctaaacag ttaccacaac ggttgcacgg
     9841 actgcttgtc tcgccaaaat agtttaggat gtattgctga agacaggact caatatacac
     9901 gtattctgtc atcgctttta atttttcata ttctttttgc ttttgccctt cttctctttg
     9961 ggactgctgg ataaaaaact tttggacttg gatatcttga ggagagaaaa gcaggatggc
    10021 ttcactggaa agaccatctc ttcccgcccg accagcttcc tgatagtatg cttctagaga
    10081 gccaggaatt tgataatgga taacaaaacg gacattgctt ttatttatac ccataccaaa
    10141 tgcatttgtc gctacaatca gctgcaagcg atcgtataaa aaggcttctt gcatttctgt
    10201 tcgctcattc tcatttagac caccatgata tctacccacg gaaaaatcga attttttaag
    10261 caaatgatag agacggtcta cttcttttct cgttgctgca taaatgatac cagattgatt
    10321 tttgttgatc tttaaatatt cgattagata ttgttctttt ttttgatctt taattacttg
    10381 aaatcgtaga ttttctcgtt caaaaccagt ctgaatatgg ttgttttctg gaatttttaa
    10441 aagtcttttt atgtcagcag ctacttgcac agtcgctgta gctgtcaaag caacaattac
    10501 aggacgattt ggcagttgat ctagctggtt cgccatctgt aaataacttg ggcggaaatc
    10561 atgcccccac tgagagatac aatgtgcctc gtcaatagcg atcatacgga cgttccatcg
    10621 cttcattgct tggataaaat ccggcatgat aaaacgttca ggagcgatat aaagtaaacg
    10681 gtactgctgt ctatcgattt cttggaagcg tctagctgtt tcgtaaccat ctaacgagct
    10741 attgataaaa gtggcgggta tacctaattg attcgccgcg tccacctgat ccttcatcaa
    10801 agagattaaa ggggacacaa ccacagtaag cccatctaat agtaaagctg gaagctggta
    10861 acagattgat ttcccacttc cggtaggcat gattcctaat acattttcct gatcaatgac
    10921 tttctggata atttcttttt gaccaggtct aaactcatca tagccaaagt attgttttaa
    10981 taagtcgttc atcttcccgc tcctttctct tgccatttta cttcgatttt cttcgaagga
    11041 caagttgatc tcctttcgtg aaacattatc ttattatgaa acaaaaactc tctagaaata
    11101 agttttttaa aagtctttta aacgtctttc ggcgtatttt ctgaatttat gtccattgac
    11161 aaagatgact tctccacaga aaagagttgg acaaaaaaag aaacc
//
LOCUS       DAYYCM010000086        10912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000086 DAYYCM010000000
VERSION     DAYYCM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.088"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            454..723
                     /gene="rpsN"
                     /locus_tag="ACHAY1_002471"
     CDS             454..723
                     /gene="rpsN"
                     /locus_tag="ACHAY1_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8594424.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            738..917
                     /gene="rpmF"
                     /locus_tag="ACHAY1_002472"
     CDS             738..917
                     /gene="rpmF"
                     /locus_tag="ACHAY1_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8594425.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            949..1326
                     /locus_tag="ACHAY1_002473"
     CDS             949..1326
                     /locus_tag="ACHAY1_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594426.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1347..1496
                     /gene="rpmG"
                     /locus_tag="ACHAY1_002474"
     CDS             1347..1496
                     /gene="rpmG"
                     /locus_tag="ACHAY1_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8594427.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1520..2503
                     /locus_tag="ACHAY1_002475"
     CDS             1520..2503
                     /locus_tag="ACHAY1_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8594428.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2533..2655
                     /locus_tag="ACHAY1_002476"
     CDS             2533..2655
                     /locus_tag="ACHAY1_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8594429.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2673..4196
                     /locus_tag="ACHAY1_002477"
     CDS             2673..4196
                     /locus_tag="ACHAY1_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8594430.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4220..4459
                     /locus_tag="ACHAY1_002478"
     CDS             4220..4459
                     /locus_tag="ACHAY1_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594431.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4652..5113)
                     /locus_tag="ACHAY1_002479"
     CDS             complement(4652..5113)
                     /locus_tag="ACHAY1_002479"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8594432.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5308..5526)
                     /locus_tag="ACHAY1_002480"
     CDS             complement(5308..5526)
                     /locus_tag="ACHAY1_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594433.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5644..7260
                     /locus_tag="ACHAY1_002481"
     CDS             5644..7260
                     /locus_tag="ACHAY1_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8594434.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7501..8841)
                     /locus_tag="ACHAY1_002482"
                     /pseudo
     CDS             complement(7501..8841)
                     /locus_tag="ACHAY1_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9279..9425)
                     /locus_tag="ACHAY1_002483"
     CDS             complement(9279..9425)
                     /locus_tag="ACHAY1_002483"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594435.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9464..9658)
                     /locus_tag="ACHAY1_002484"
     CDS             complement(9464..9658)
                     /locus_tag="ACHAY1_002484"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8594436.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9568..9900)
                     /locus_tag="ACHAY1_002485"
     CDS             complement(9568..9900)
                     /locus_tag="ACHAY1_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594437.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10414..10605)
                     /locus_tag="ACHAY1_002486"
     CDS             complement(10414..10605)
                     /locus_tag="ACHAY1_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594438.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10699..>10912)
                     /locus_tag="ACHAY1_002487"
     CDS             complement(10699..>10912)
                     /locus_tag="ACHAY1_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8594439.1"
                     /translation="INVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3639 a         1724 c         2107 g         3442 t
ORIGIN      
        1 ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga attagttcac
       61 ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg gcgataaacc
      121 agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc gttttaagta
      181 gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag tgaattctta
      241 tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta tgtaccgata
      301 aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat ttattgttga
      361 caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac gatttgaggt
      421 gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa aattgctaaa
      481 gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga attaaaagcc
      541 gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa tcgtctgagg
      601 cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt
      661 attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg
      721 taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg aagaaacggt
      781 taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg
      841 attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag aaagtaatta
      901 aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat ggaaattatt
      961 tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca gcaatcgcta
     1021 gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga aacgggttta
     1081 ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga ccaaggctta
     1141 tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga ggagttattt
     1201 caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt agtttcacaa
     1261 ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata tcttgaagat
     1321 cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa
     1381 ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa cggttagaat
     1441 taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg aaatagctaa
     1501 gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt ttttaggggc
     1561 tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca gggaagaaat
     1621 tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat tagttcatag
     1681 cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc gcagtgattt
     1741 attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac caatttcaca
     1801 aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa cacttttaac
     1861 agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta ttgatagtga
     1921 cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca tggcagatcg
     1981 catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat tacagagaga
     2041 aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg aaacgattca
     2101 aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg aaagtgattc
     2161 atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac atgagtttaa
     2221 atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc aatggattga
     2281 ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga ttgctttaca
     2341 agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt ttcaatacac
     2401 cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg atgaagaaag
     2461 gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag tagtgattgg
     2521 gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact taaaagcccg
     2581 aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg aaataaaatg
     2641 aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg
     2701 ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca aaagtatcga
     2761 atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat gatttcacca
     2821 aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga tcagaacctc
     2881 atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat gtttttgtat
     2941 atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg aagaaagctg
     3001 ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt gaagaagaag
     3061 atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg tgggtttcac
     3121 ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa ttgtatccga
     3181 aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg aaacggttgg
     3241 atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg acgcaacacg
     3301 cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt gctggactaa
     3361 gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa
     3421 ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca aaaactttgg
     3481 ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt acaaaagaac
     3541 aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa gcgttagaaa
     3601 aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga
     3661 cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg agtgattata
     3721 caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat gaagttttcg
     3781 attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat tattatacta
     3841 ctggctataa gacagatatt gatactatca acattaaaga caacaccatt gattttgtag
     3901 tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt ttgaattatg
     3961 aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt
     4021 ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag catttccata
     4081 ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat tggcctactt
     4141 tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct cattaaaatt
     4201 caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt ccaagaatat
     4261 tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga gtacattagc
     4321 tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat taaaacggga
     4381 tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag caaaacggca
     4441 taatagcttg gccaattaat tgataatacc agagtagatc atttttccct tatttggatt
     4501 tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt atcaaagaag
     4561 acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag tttttcaatc
     4621 atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg caagtattct
     4681 cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga ggatctggaa
     4741 aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag aattcttttc
     4801 ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa agttcaatca
     4861 ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct aaaaggaagc
     4921 atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca gctacaatat
     4981 cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg ccatggatcg
     5041 aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt cttgtttttc
     5101 ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt tctgtatcga
     5161 aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag tccatatgtt
     5221 catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca aataaatgac
     5281 gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat ttttttcttc
     5341 gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt ttcttctcag
     5401 ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac taatagaaaa
     5461 tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat gacgatactg
     5521 gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt tggatatcga
     5581 ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag aaagaggttc
     5641 cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg aaaaacgata
     5701 cttctaaatc atattctgta cggtgatcac ggaaagaaaa ttgctgtcat tgtaaatgac
     5761 ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa aagaactgga
     5821 gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga agatttgatt
     5881 gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat tgaagcttca
     5941 ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa ccctttagac
     6001 attgatttga cccaacgatt agagattggt gccattatta tagtagtgga tgtaaaacgt
     6061 ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga aggtaatgta
     6121 gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg ttgtaacata
     6181 ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat taacggtcta
     6241 attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga agtagactta
     6301 tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc agtgggctgg
     6361 ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga atatggtatc
     6421 tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc ccatcttacg
     6481 gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt tgcccaacat
     6541 agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga atcattaaat
     6601 tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa aaatcccgaa
     6661 ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt ttttattggt
     6721 atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac tgattctctt
     6781 aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa tatgtttagc
     6841 cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga tgtcataatt
     6901 attggcattt tgattattac ctcttttatt cccttatttg tattctctat agaaaaggag
     6961 aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg caaggaaatg
     7021 gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc tgcgaaggga
     7081 caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga taatagtcct
     7141 gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag tatttgttta
     7201 ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta catttactaa
     7261 tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa taatagcagg
     7321 ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga caacaggcac
     7381 aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt acttattttg
     7441 gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat actcacacgg
     7501 ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt gggtagtatt
     7561 cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt agttttgaaa
     7621 gtttctatag ccataggcaa ttcgttttag tactttgata tggttattca aacattctat
     7681 agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt atttcttgaa
     7741 ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt ttaggagcgc
     7801 gacgaattca ggaacatctt gtcttctgat aacggaaaga aaatcctggt agacttcgta
     7861 gcctcgcttt aaggaatcat cataagataa tagccgatcg actatctctg cttctgtcag
     7921 atgatctcga aatgacggac gccagtagcg atgctgacct ttcaattccc acgcattctt
     7981 ttgaagtagt ttccagtatt tctttaagtg cttcgaccga cgatcccctt ttttaaaggt
     8041 cttcattact tgaattcggt ggttggtaaa agcccgacca atgtgttggc taatatgaaa
     8101 gcgatcggcg acgattcggg cattcggaaa acactctttc accaatgtgc gatagggtgt
     8161 atagaaatct gaaaccacta gacgcacttg ttcacggact tttatagggt agcgcaagaa
     8221 atagtcgcgt aaaggattga gttgccgatt ttccacaata tcaatcagtt gatgggtctt
     8281 gccgtccatc atgacaaagc tcatggctcc cgagacattt ttcactgatt tgaattcatc
     8341 gaaacacacc acttctggta acagctggct caaagaaatg gttttaggcc gaaggctgcg
     8401 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
     8461 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
     8521 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
     8581 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata agatggatac
     8641 ttttttccag ccctatttaa tgatcgtttg ttcattttga tagtggcgat gaaagcattc
     8701 ctccggttga taggtcaatg tgccatcaaa aacaaaacat gtttcgtcat tgattcgctc
     8761 tttgcgaaag gcatcttcat gaaagataag atttaggtct agaatatcta gtaactcttt
     8821 ggtaaactct gaataggaca tcttgaaaca ctcctttgaa tggttttcgt cgactttatt
     8881 ctaaaggatt ttaaggtgtc tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca
     8941 gaaggcgtca ccaacactag atattataga tcccttattt tcttcgcttc aaattgtcaa
     9001 ggagtcatca taaattttgt atggattctt ttgtttgggt agtagtcttc taaataatct
     9061 tggattaaca ccttcatctc tattttactt ttatatttgt tggagtcgat taattctcat
     9121 ttgattgatc gatgaaacgc ttcaattact gcattgtcaa caggagtccc agtttggctc
     9181 actgaatgtg tgatgtggtg gtcgttcaac agctcttgat aaataaaaga ccgatgctgg
     9241 ctacctctat cggaatgaat taccgtaggc aattctcctt aataaatttg taatggacgg
     9301 acgacaatct cttttttcat gtgtgaatct accatatagt aacgtagaat acgagttgct
     9361 aagtcaatat aggtggacaa atagaggcga ccgtcattac atggaatgta agtaatatcc
     9421 atcacccaaa cagcatcaat ttggttctgt tcaaattatc gattcaacat ttttttacca
     9481 tttctttgat tgttttttct ctaccatgag aagatttgcg tcgatgaaat cctcttgtgc
     9541 ataaattcat ttcacgcata agaacagcta caacacgttt attgatttcg attccttcat
     9601 cgtaaagttt ttgagtgatc cctggagatc cgtatcgatc tttgtgttcg tagaacactc
     9661 gtcttcctct tttagtcgtt tattttcctg ttcaagaatg tagaaatctg ctttatcagg
     9721 taatcttttt ccttgtccag caaaagcatt tttcccgtat cgacgatatt ctgaaaccca
     9781 tcgggaaatt gttggacgag agatattgta ttcttcagca attttcttgg tagttacttt
     9841 tttctcaagg atatcctgtg aaatctttag tttaaattta cgatcatatg tttttctcaa
     9901 tagagttcat tccttccggt aacgtaactg atagtcaaga aaagtgtatc atagcccaac
     9961 ttagtatcag ggtttgtcaa ctaaactgtg gaagttgata gataagagtt tttaacaacc
    10021 aaaattctct tgactatttt tgaaatcaga caaattttga ttggacacat agttgaataa
    10081 atctaccgtc gttacggaag gtaaatcgca tgcttttcaa ttctagagga gaagtcctcg
    10141 ttgaagtacc atagcccagt tctggattcg acctcctgcc cacttcatac gtaaataaag
    10201 taattttagg agtgagactg tgacaaaagt tatttttaag ctaaaaacga attaaaaaac
    10261 gttgataatt aaaatcattc gttttagatt ctatgaatca ttatagaacg ctgtaaaatc
    10321 gatactttat aattataaag aagatagttt caaatttatt gttcgaaatt tgaaactatc
    10381 tttttactta tgtcacagac tcccttagtt gagttattct ttaatttcga atttcgagat
    10441 agctagatat attgcaagca tacattcttg ataacaatct tgatcatatt ttccagttct
    10501 ccaggattgc ttatgaatca aagagctaaa tcttttgaga attgcctcaa cagcttcttc
    10561 atttccagcc tttgctaaaa tgaaatcttc ttttaaagaa ttcataaatc taccttcttc
    10621 ccatggactt ataatctatt tctttgggaa ggaggagaac gagcaaagaa gcatacaaaa
    10681 ttagttatta tgcagatatt aaaccagcat tttttgaatt tttttgtaaa gtcgatgttt
    10741 taaatttgtc gcaccctgtc ttgtaatccc cagaaccttt ccaatttctt tatcagtttt
    10801 atcaaagata aatttttcga taagaaaaat ttgttcaggt tctttcaaca aaggaaggat
    10861 ttttgctaat tttatatctt caacaaggag agtcacattt aaaacattaa tc
//
LOCUS       DAYYCM010000087        10351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000087 DAYYCM010000000
VERSION     DAYYCM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.089"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..285
                     /locus_tag="ACHAY1_002488"
     CDS             136..285
                     /locus_tag="ACHAY1_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8594440.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            296..1810
                     /gene="dltA"
                     /locus_tag="ACHAY1_002489"
     CDS             296..1810
                     /gene="dltA"
                     /locus_tag="ACHAY1_002489"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8594441.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1807..3012
                     /gene="dltB"
                     /locus_tag="ACHAY1_002490"
     CDS             1807..3012
                     /gene="dltB"
                     /locus_tag="ACHAY1_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8594442.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3057..3290
                     /gene="dltC"
                     /locus_tag="ACHAY1_002491"
     CDS             3057..3290
                     /gene="dltC"
                     /locus_tag="ACHAY1_002491"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8594443.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3293..4561
                     /gene="dltD"
                     /locus_tag="ACHAY1_002492"
     CDS             3293..4561
                     /gene="dltD"
                     /locus_tag="ACHAY1_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8594444.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4702..5784
                     /locus_tag="ACHAY1_002493"
     CDS             4702..5784
                     /locus_tag="ACHAY1_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8594445.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5784..6587
                     /locus_tag="ACHAY1_002494"
     CDS             5784..6587
                     /locus_tag="ACHAY1_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8594446.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6657..7397
                     /locus_tag="ACHAY1_002495"
     CDS             6657..7397
                     /locus_tag="ACHAY1_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8594447.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7408..8412
                     /locus_tag="ACHAY1_002496"
     CDS             7408..8412
                     /locus_tag="ACHAY1_002496"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8594448.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8457..10301)
                     /locus_tag="ACHAY1_002497"
     CDS             complement(8457..10301)
                     /locus_tag="ACHAY1_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594449.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3326 a         1707 c         2090 g         3228 t
ORIGIN      
        1 gatttgatgt gctattttta taaaagctat gtgacaaaac tgttagtaaa aaaacggttt
       61 ctgtaagcta gacacatgtt tctttttgtc aaaattagtt acactttgtg tgaggttata
      121 tgaggaggga atccaatgaa caaatggcgt tctaatccaa aattgaatta ttggggaaaa
      181 ttcatcattc gaacaatttt ttatagtgca gtactgctgt tcctgattta tctgtatcac
      241 tataaaaata ttcaaggagg aacttttatc tataacgaat tttaggagga agagtatgga
      301 aatcaaaacg attattgaag caatcgatga ttggggaata aatgaacctg atcgagttgc
      361 ttatcaggcg gatgagacgc atacttttgg tgaattgaaa caagcatcag acgcacttgc
      421 ttattattta gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg gcaatttgga
      481 gtttgaaatg attgtctcat ttctaggggt tgtcaaatct ggccatgcct atttgccgat
      541 tgaagagcat acacccaagg agcgtatcct atcgatcttt cgggtggcta aaccatctat
      601 ggtcatcagt atcggggact ggatagaaga tattccttcg gttcctgtca taacaaaaaa
      661 agaatttcaa gaaatcaaga agataccagt aggattttat cccagaaata gcgtaaaagg
      721 tacagaaaat ttttatatta tttttacttc tggtactact ggagaaccaa aaggcgtaca
      781 aatcagtcat gataatctag tcagctttgt tcaatggacg atggaagact ttgggatcaa
      841 gcctggtatg catttcttag cccaagcgcc attttctttt gatttatcgg tattttctat
      901 ttatcctgca ttagtttctg gcggaatgct gaagccgtta aataaagcag ttgttcaaga
      961 ctttcgtcaa ttattcgcga cattgccagc cttgaagttg aatgtgtggg tttctactcc
     1021 atcatttatg gatatttgct tgatggaacc tacattcaat ggagaaaatg tgccggaatt
     1081 ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa gtctacttga
     1141 tcgattccca aatgcacgga tatataatac ttacggtcca acagaagcaa ctgtagctat
     1201 ttcaagtatt cagattgacc agcatgtact agatagttat gaccgactac cgattggcta
     1261 cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt aaacctgttt caaaaggaga
     1321 aacgggtgaa atcattattg caggaccaag cgtatcaaaa ggttacctga acaatcccga
     1381 aaagacagct gctgcgttct tttgtcaaaa tgacttagca agttaccata caggggatgc
     1441 aggtagacta gatgaatatg ggcttttatt ttatgaagga cgtatggatc agcaggtcaa
     1501 attacatgga taccggattg aactcgggga tatcgaacat tatctgctcc aagacaaccg
     1561 aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc agctggttgc
     1621 ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt cagttgacta aatctatcaa
     1681 agaacaatta ctagaagttg taatggatta tatgattcct cagaagttcc attatgtcga
     1741 tctgctgcct cagacagtta atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa
     1801 tcctgaatga tgttacctca tatgatccct tatgcagagc cgttttattt ttttctttta
     1861 gccattgcgc ttgtaccgat cgttctatct ttattgatat gggaaaaaag attaccagtt
     1921 tatcaatcag cagtgacact tttcttttta tttgtcagct ttggaggtaa ttattggcaa
     1981 cagggagcgg cgctgattgg gtatgttttg tggcagacga tacttgtatg gggctatttt
     2041 gtttatcggc aaaggaaaaa tcagacagga tggttttttg ccgccgttat tttagcgata
     2101 attccactgt ttttaacgaa agtgactcct ttttttgaac atggaaaagc atcgttactt
     2161 ggttttttag gaatctctta tttaaccttt aaatctgtac aaatgatcat ggaaataaga
     2221 gatggcatga tcaaaacata taagccattt cgttatatcc agtttctgct gttctttcca
     2281 acaatctctt ctggaccaat cgatcgctat aggagatttg aaaaagatca gctccatccg
     2341 cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt
     2401 tttttatata aattcattat cggttattat tttggtcaag tactgcttcc tgtagtggga
     2461 cgcattgcct tacagcatgg tggtatttca tgggcattag taggttatat gtacgtgtac
     2521 agtatgtatt tgttttttga ttttgctggc tatagcttat ttgcagtcgg tacaagctac
     2581 atgatgggat atcagacacc aatcaatttc aataaacctt ttttatcatg gaatatcaaa
     2641 gaattctgga atcgttggca tatgacgctg tccttttggt tccgtgatta tgtatatatg
     2701 cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat
     2761 atcggttatt ttgctttgtt tcttctaatg ggtatatggc atggattgac gtggtactac
     2821 atcgtatatg gactgtatca tgcttgtctg atctgtctga cagatgcatg gatccgattt
     2881 aagaagaagc ataaagagca gttgccttct aacaaattta cgcatggctt agccgtattt
     2941 ttgacatttc aagcagtatg ttttagcttc ctgatttttt caggattttt agataaactt
     3001 ttttttagct aacgaatagt gacatattaa tttatataac aaaggagaaa aggaaaatgg
     3061 aagaacaaat tttatcaatc ttagaagaaa taacaggaac agatgaagta agaaaaaatc
     3121 gtgatattga tctatttgat gaagggttgc ttgattcttt agcatctgtt caattattag
     3181 tcgagctaga cggagagctg gatatccaag tccctgtatc tgaattcgag cgagaggatt
     3241 ggtgcacacc gaataaaatc attcaacaag caactgcatt gaagggttga gtatgaaaaa
     3301 gaaacttttt gctattttcg gcccggttgt tttagctgtt ggcttactgt ttgtgttttt
     3361 tacctcctcc tatcaagtag atatctctaa tccagaaacg attagacgtg cctcttcttc
     3421 catgtcagtg aacgtgttga aaggagatgc gataaaaaat tatgcattat ctgaaaaaca
     3481 atatattcct ttttttggct caagtgaatt aagcagaatc agtccatttc atccgtctgt
     3541 tctggcagaa aaataccagc gaaactatcg tccatttttg ctgggagcac ccgggacaca
     3601 atcgttaagc caatatatga tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt
     3661 cgtctttatt atttctcctc agtggttcgt taaaaatggt gtaaaaactg attactttaa
     3721 tacgtattat tctgaacttc aaacatacga ctggttgttt tcaatgaaga aagtaacgcc
     3781 tgctgatcgc tatttagcta gaagattatt aactttctca aaagtaaaag agaatgatac
     3841 gttgacggcg attctgcaaa cgatcaaaaa agggaagcta cctttacctg aatctttgaa
     3901 tcagctgcgc agccaatgga acatgctgaa gagagaggat gaagtattca gcaatattgg
     3961 gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa aacaatacca
     4021 agaaacagaa ttatccattt tagcaaatca aattggagaa cgagaaacga cgaataatcc
     4081 attcgggttg aaaaacgatt tttatacgca tcgtatccga gcccacgagc cagagctcaa
     4141 acagtcacaa aaaaattggg attatcgttt ttcgccagaa ttttctgatt ttcagctggt
     4201 cttggatcaa ttagcaaaaa accataacga agtattgttc atcatcccac cggttaacga
     4261 gaaatggagc gattataccg gactatctca agaaatgtta caaggatttg ccaaaaagat
     4321 caaatttcaa ttaaatagcc aaggattcaa tcgaatagct gattttgtca atcaagcggg
     4381 taccaactat tttatggaag acacgatcca tctaggctgg aaaggctggt tggctgcaga
     4441 tcaacagatc agaccgtttt tagaagaaaa tcatattact gctagtaagt atcatcttga
     4501 cgatgctttt tttagcaaat cttggcaaca tcaaatacca gataaactac aattaaaata
     4561 aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc tgaataaaag
     4621 ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa tggtataatc acaatatgaa
     4681 tatagaaatg ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact
     4741 tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc
     4801 aaaccattcg ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt
     4861 gctggcggag gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta
     4921 acaaaaataa ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa
     4981 gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg
     5041 attggttcca aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata
     5101 aaggatatgt tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata
     5161 cgtcctggag atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat
     5221 ttactggcaa aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag
     5281 aatctatcta aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa
     5341 gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct
     5401 ttagatactg gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt
     5461 ttaggaaaag gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta
     5521 ttgaattttg ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt
     5581 tctggcggag gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta
     5641 gccattaccg ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat
     5701 tatttgaata cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt
     5761 gaaaaaattc ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa
     5821 aagcacgagt ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta
     5881 taatatttac gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa
     5941 gcatgcagca gcacttattg agttttctag tcgataattt acttttattc gcattcaact
     6001 atgcattgtt ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt
     6061 tattttcagt tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt
     6121 cagtcatcaa ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag
     6181 tgagtactta tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag
     6241 aagattttct tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac
     6301 tcatcatgag tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc
     6361 ctgatttgtc ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg
     6421 gtacgtatat tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt
     6481 tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg
     6541 aacaagcacg gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt
     6601 tattcatcaa aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga
     6661 aattaactgt tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt
     6721 atttgcttca atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca
     6781 aattacaaga acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc
     6841 atgatcatat tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa
     6901 atccggagac attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat
     6961 tgacgttgga aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg
     7021 gtccttttct gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta
     7081 tcgtagaaga aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa
     7141 gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag
     7201 gaaatgaaca tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag
     7261 ctaacgtaaa gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga
     7321 aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca
     7381 aagtgtttaa catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca
     7441 agaatcaatt tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt
     7501 ttgctctttt atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa
     7561 gaaatcaatc gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga
     7621 ggcggagctg tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat
     7681 ggggattcat tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa
     7741 ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa
     7801 ttttctggca acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg
     7861 ttcgactctg atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa
     7921 tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa
     7981 gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc
     8041 gtagagtcag tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc
     8101 aatcgaatct ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat
     8161 ttcaatttct ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc
     8221 tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc
     8281 tctgacgtgg aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata
     8341 gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc
     8401 ttactttatt gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat
     8461 acaggattcg ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt
     8521 tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa
     8581 cgttcctgat catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt
     8641 gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt
     8701 tttcgataca gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg
     8761 atcaaaagtt gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg
     8821 aaggttatct gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt
     8881 gtcacctggt ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc
     8941 ttttccacat agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc
     9001 atctcttgga caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc
     9061 tcataatgtt gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct
     9121 ttgtcggtat ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata
     9181 atatataaaa tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg
     9241 taaatcaagc aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa
     9301 cggttattct tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca
     9361 ctagcaatat tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa
     9421 agcagaacgc cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta
     9481 tagtaaaaca ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat
     9541 aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct
     9601 gatacagctt caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca
     9661 agacgttttt gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa
     9721 acagcgatta tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat
     9781 ggtccagttg ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat
     9841 gagactgtaa gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat
     9901 gagagtccgg gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct
     9961 agatatacag taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt
    10021 ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga
    10081 gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct
    10141 tttatttctt tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca
    10201 attcctgtta aaaataacaa tgataatata atgattatgc tccactttat acgtaaataa
    10261 aaagattctt ttttattata tatgttatta aaaaaagaca taaaggtatt cctttctaaa
    10321 acttgtaatg ttagggtaaa cgttttttaa a
//
LOCUS       DAYYCM010000088         9945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000088 DAYYCM010000000
VERSION     DAYYCM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.092"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHAY1_002498"
     CDS             complement(<1..613)
                     /locus_tag="ACHAY1_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8594450.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACHAY1_002499"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACHAY1_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACHAY1_002500"
     CDS             complement(2207..2542)
                     /locus_tag="ACHAY1_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8594451.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACHAY1_002501"
     CDS             complement(2526..2789)
                     /locus_tag="ACHAY1_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8594452.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACHAY1_002502"
     CDS             complement(3249..4052)
                     /locus_tag="ACHAY1_002502"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8594453.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACHAY1_002503"
     CDS             complement(4030..5154)
                     /locus_tag="ACHAY1_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594454.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACHAY1_002504"
     CDS             complement(5170..5985)
                     /locus_tag="ACHAY1_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8594455.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACHAY1_002505"
     CDS             complement(5975..6847)
                     /locus_tag="ACHAY1_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8594456.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACHAY1_002506"
     CDS             complement(6932..8221)
                     /locus_tag="ACHAY1_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8594457.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8877)
                     /locus_tag="ACHAY1_002507"
     CDS             complement(8365..8877)
                     /locus_tag="ACHAY1_002507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594458.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8944..9087)
                     /locus_tag="ACHAY1_002508"
     CDS             complement(8944..9087)
                     /locus_tag="ACHAY1_002508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8594459.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9570..>9945)
                     /locus_tag="ACHAY1_002509"
     CDS             complement(9570..>9945)
                     /locus_tag="ACHAY1_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594460.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         3054 a         2110 c         1610 g         3171 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 agccagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
     9661 tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag
     9721 gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt
     9781 tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt
     9841 cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga
     9901 tgttccactt catccatcag atctccccaa acatgtctcg tgatg
//
LOCUS       DAYYCM010000089         9474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000089 DAYYCM010000000
VERSION     DAYYCM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.093"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1013
                     /locus_tag="ACHAY1_002510"
     CDS             <1..1013
                     /locus_tag="ACHAY1_002510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8594461.1"
                     /translation="DFIILIRVRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGS
                     QAVLMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATK
                     KAREFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLD
                     GSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYST
                     AACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKF
                     SNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQM
                     KY"
     gene            995..1456
                     /locus_tag="ACHAY1_002511"
     CDS             995..1456
                     /locus_tag="ACHAY1_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8594462.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1506..1970
                     /locus_tag="ACHAY1_002512"
     CDS             1506..1970
                     /locus_tag="ACHAY1_002512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8594463.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1984..2733
                     /locus_tag="ACHAY1_002513"
     CDS             1984..2733
                     /locus_tag="ACHAY1_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8594464.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2733..3563
                     /locus_tag="ACHAY1_002514"
     CDS             2733..3563
                     /locus_tag="ACHAY1_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8594465.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3563..4210
                     /locus_tag="ACHAY1_002515"
     CDS             3563..4210
                     /locus_tag="ACHAY1_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8594466.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4308..5084)
                     /locus_tag="ACHAY1_002516"
     CDS             complement(4308..5084)
                     /locus_tag="ACHAY1_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8594467.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5228..6187
                     /locus_tag="ACHAY1_002517"
     CDS             5228..6187
                     /locus_tag="ACHAY1_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8594468.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6304..6612
                     /locus_tag="ACHAY1_002518"
     CDS             6304..6612
                     /locus_tag="ACHAY1_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8594469.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6639..6938
                     /locus_tag="ACHAY1_002519"
     CDS             6639..6938
                     /locus_tag="ACHAY1_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8594470.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7112..7663
                     /locus_tag="ACHAY1_002520"
     CDS             7112..7663
                     /locus_tag="ACHAY1_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8594471.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7809..8102
                     /locus_tag="ACHAY1_002521"
     CDS             7809..8102
                     /locus_tag="ACHAY1_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8594472.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8095..8391
                     /locus_tag="ACHAY1_002522"
     CDS             8095..8391
                     /locus_tag="ACHAY1_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594473.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8462..9466
                     /locus_tag="ACHAY1_002523"
     CDS             8462..9466
                     /locus_tag="ACHAY1_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8594474.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3344 a         1366 c         1781 g         2983 t
ORIGIN      
        1 atgactttat aatacttatt agagtgaggg ataaagaaat gagacaacta cacaaagaac
       61 agattgaaac agcaatggca gcgttaaagg aaaagacaat tgatcatgta tattttacag
      121 catgtggggg ttcacaagcc gttttaatgg ctggtcaata tctatttgat aaagaattaa
      181 ctgtaccttg tcatgtttat actgctaacg aatttaacta tgctatacca aaaggattat
      241 ctgaaaattc tttagttatc acttgttccc attctggcac aacaccagaa actgtagaag
      301 caacaaaaaa agcaagagaa tttggagcaa tgactatttg tttatctaat gaagaaggat
      361 ctcctctatg ggaagcagct gaatatcctg ttcactatga ttggggtaaa gatgttgatg
      421 ctagtgattt aaataaagga attctttatg gtttattatt taatatttta cttgttttag
      481 atggctctaa aaaatgggaa acttgcttga atgaatttga taagttaaat gatcttgtta
      541 ttgctgaaaa gagcaaattt gaaataactg ctaaagaatg gggtaaaaac caaaaacgag
      601 ataaaattat ttacacaatt ggtagcggta tcaattacgg agaaatttac tccacagcag
      661 cttgctggtt catggaaatg caatggatta attctaatgc tattcattct ggggaatttt
      721 tccatggtcc ttttgaaatc actgattatg atgtaccatt catcttagtg aaaagtattg
      781 gagatacaag atttttagat caacgggtag aagacttcac taaaaaattc agtaataaat
      841 taatggtttt agaccaagct gattttgact tagaatctat agatagtgta gcgaaagaat
      901 atattgcagc tgtacttagt ggcgttgtta ttcgccaaat ggtagaagcg attgcttttg
      961 aaagaggcca ttccttagat gttagacgct atatgtggca aatgaaatat taattaatag
     1021 gaggttgcaa aagatggcaa atcaattatt tcacattgtg attacaacac attctaccct
     1081 ttgtgagggt tatttaaagg caacaaaatt aattttaatg aatgacgcca caggtattag
     1141 tgctgttcca tttaaagaaa atactagtcc agatgatttt gaggagagta tgaaagaaat
     1201 aattagtcag catcatttgc aacctctaat tattttaacg gatttaattg gaggaacacc
     1261 taataatgtt tctattaaac agttgaacaa caatagaatt caaattattg ctgggattaa
     1321 tttacctctt ttactggagt tattaatggc ccagtcagct ggtaaatcta tagaagaaat
     1381 agattttaag gaattactga ataactctca aaaaagtatg gttcatatta attcgctttt
     1441 aaaagatgtc agttaaggtg gcaatttcta ttctataaat tttaaaaatt gaaggaggaa
     1501 atagaatgat taaattagtg cgtgtagacc acagattact tcatggacaa gtaatatttt
     1561 cttggactaa acagtatgat attaaccata ttattgtggc tgataacaaa gttccaggag
     1621 atccaatgtc tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa ttagacattg
     1681 ttaaaattag tgatgttaaa gggattatgg aaacatcccc aaatgataat tacatgattt
     1741 taattaaagg acctaaggaa gcttttgaat tagttcaaaa tttaccagaa attaaagaaa
     1801 ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag gcagtctttt
     1861 taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg aaaatatttg
     1921 tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa ggggggaaaa
     1981 taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt tattgactcc
     2041 aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact tgccggactt
     2101 gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt aatttctatg
     2161 ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag tattattgcc
     2221 actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat agctatccca
     2281 gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc tcaatttgga
     2341 catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat tattcatatt
     2401 tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt aacagtatat
     2461 tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat tacagacgcc
     2521 ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt aagcacaatg
     2581 ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc ctattcaggt
     2641 ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat ggatcaagtt
     2701 aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt
     2761 taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc atgtgttcgt
     2821 ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca attttaaata
     2881 aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat atggcatttt
     2941 ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct atggaagaac
     3001 aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg attaaaacca
     3061 gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact ttaagaatta
     3121 ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga ccgattttag
     3181 ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt aaattaggat
     3241 atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg gatcgagtaa
     3301 tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct agcatggtgc
     3361 caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta caagacttac
     3421 ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata tataaattga
     3481 ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca gggtttgttt
     3541 taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg acggagtttt
     3601 aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac aagaaaattt
     3661 aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta tttttgatga
     3721 tatagaaaag tacaatgagg gatgtaacag tttagaattg aagggaaaat ttactaaata
     3781 ttggaaatcg caaaagattg attacttgga tatttttcct aatatactaa ttgaacagct
     3841 tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc caatggatac
     3901 tataaatgaa gttgttacca cttgtaaaat agagtcatat tttgattgtt taattagcgg
     3961 aagagatttg ccagaaagta aacctaatcc tactatattc cttaaagctt cagaacagct
     4021 cagaattcca gttgaagaat gtctggtgat agaagattct tataatggta taaaagctgg
     4081 taaaagagca aatatgaagg ttcttgcaat taaagataaa cgtttttctc aagatgtaag
     4141 tctagcagat gatgttattt acgatattaa atatctgcga caagaagtac aggtgaaatt
     4201 tggaatataa ataagttttt tgatgtattt agagaatatt agtaaaaata aaaatcatgc
     4261 ccaaatctaa tcaaaattga ttgctttggg tatgatttct tttaatatca atctattact
     4321 ttaggatctg aatcattttt atgcaccgta tctaaagtga tattttctgt gacttcaata
     4381 acataactaa ttctatcaca acgaactgtg gattgagaat aatgaaatgg ttgattattt
     4441 ttatctttgc caaccttttc taaaataaat agcggatctc ctactataca ttgcaataag
     4501 tcggaaagct caggtgttgc agataaaccg tttatttcca taatagagct cgtagctttg
     4561 acatcatagt catcttctag aagtttatat aatgaagtat attggctacc tttttcaaat
     4621 aaagttggaa atttttcttc tgatgcgaaa aaattatcaa ttcccatcgg ggcaccgtct
     4681 tcccacatta aacgtttaat attgtaaact ctatcagtta cattgatttg taatttatta
     4741 gctaaaaact catcagcctt tataatttta gaatgtaaaa cagtatatct aattttatgt
     4801 cctcgggtag aaagagaatc gctaaatcca tgaactccag ctaattttaa actttctttt
     4861 ttttgagtta caaaggtccc tttccccctt aatcgttcaa ctgttccact ttctgctaaa
     4921 agtttaaaag cttttcgaat ggtaatctta cttactccat aaattttaca taattcttct
     4981 tctgtaggta attgctctcc tggaagatat tttttttcac gaatatcgtt acgtatagaa
     5041 gcttctactt gtaaatataa tggtctatta tcttgaaaat tcatagtaca ctccctaaaa
     5101 agttatactt cattatatca caatttatta aaacgtcgat tgtaaaatta agctagacaa
     5161 ctaaaaagat catttgtgct acactcaaaa tagttccgac caaagaatta ataaggagtg
     5221 agtacaaatg acttacaccc atcttacaac agacgaacta gtaatcatag agtcttattt
     5281 caatatcgag aaacctgtcg ctcttgtcgc acaaagttta aatcgctcta gacaaaccat
     5341 ttataatgtt tattcgtttc tcaaacaagg aaaaaaagtg ctagattatt accgtcaata
     5401 caaacaaaat aaatctcgtt gtggacgacg ttcaaccgtt ttatctgatg aacaatcaac
     5461 atatgttcaa gaaaaggtag ctcaaggatg gtcacccgat gtgattatcg gccgtggcga
     5521 gtttccaatt gattgttctg ttcgtacact ctatcggatg tttaaaaaag gcctttttga
     5581 cacacagtcg ttacctatga aggggaaacg taaaccgaat aatcatcaag aaaagcgtgg
     5641 aaaacaggcc tttcgcagaa cgattcatga acggaacagt ctctatccac acctgactaa
     5701 agaatttggt catctcgaag tcgacaccat tgtgggagtt aaacacaaga gcgctgttat
     5761 tacattggtt gagtgtttat ccaaggttat tattactttg aaaccgacag gcagacgagc
     5821 cgaagatatc gaaaacaggt taaatgattg gttcaaatcc atgcctaaac acttgtttaa
     5881 atccatgaca tttgattgtg gaaaagaatt ttccaactgg cgatctataa gtaactcgaa
     5941 cgatattgcc atctttttcg cagatccagg aacaccttcg caaagagggc taaatgagca
     6001 ctcaaacggc ttgcttagac gagatggctt acctaaaaca atggacttta gggacacgga
     6061 tgaaaccttt attcaatccg ttgcctcgaa aagaaaccat attccaagaa aatcattaaa
     6121 ttatcgaaca ccattggaag tatttttgag ctgcatagat aaagacatat tgtctagctt
     6181 aatttgacaa atcagatagt ataaaatgaa tcaaattgaa tatcaaatat atattatatc
     6241 catgaagccc aatcttcttt aatagaagat tgggctattt ttatggcaaa ggagggaaaa
     6301 gaaatgtttc aaaataaagg acaacgttat gtcacaaagg gagtaatgga cagtttgccg
     6361 gtggaattac aagctttatg ttggaatcta atcgatcaaa acgttcaaaa gcaatttcat
     6421 ttagactatc ttcaaatctt tgaattttct acggaaaaag gaaatcaaaa gttggttcat
     6481 cgtcaagaag aaccagaagc aagaaaagag tatctgattt cacctaagct tcgtttgaaa
     6541 tcagtgtctc aaaaaatatg ggtcattgat tcgggtgagt atcaaacgat gttattacca
     6601 gaagaatact gatatctaaa agaaaggaac gaaaggactt gttagaaacg gctatcgaag
     6661 tactggaaaa gtgtgcacag ctagtcacta ctcctgaaga gtggggctat gaatccgtta
     6721 cgatggaaaa agaggaaatt gaaatgggta tgcttcctga agatgttact ctaccacgtt
     6781 tagtgatgac acatctttat atttattgcg caccagaaga tggaaaagac tatgtagttt
     6841 atttcattac agatattacc agccagcgag aatttgttcg tgggttactg gtagaaggac
     6901 gattggtttg gagtcagatt ggaggaacga atgaataaga tcgattattt gagaatgatt
     6961 gcgaaatgtt tagaagatat ccttacagcg attcatcaaa ttctgaagaa aaaaggagaa
     7021 agaatatgaa atattttcaa ctagtattga cacttataac ggcaatggtg attgaattgc
     7081 ttgaacaaaa attagcatag gagaaaaaga gatgattgaa aaagaattag tgaccacaca
     7141 aacagaagtt tggcgagatc ctcatcatac ccatcgctat gttttcaaac gacaatgggc
     7201 gatcaaagga aaagaagcgg aagataaatt agcggtagtc attactattc gtccaacaga
     7261 tacagaacca tttacgaatg atttgagtat gttattaatc gaaaagaata tccgacaatt
     7321 aggatttact ggttttattg ctgtgaatct tttttcgtat acaaaagcga aaagtcctcg
     7381 ggtatttcct cgtggaaatg atgaacaatc tttggaaata ttaacaactg ttttcacaga
     7441 aaagaaaatc aacaccatta tttttgcttg tggttcgatt acagcgacga gtcaagtggc
     7501 ttatgaacaa gcgaagacca tttacgatca attgagtgcg aaacaaaaaa agatgaccaa
     7561 agtattgata aatgcagaag gtaagttatc ccacccacta agtattcatg ttcgtaatga
     7621 atggcaattg gcagatagtc aattattatt tcaagaaaaa taacatttta ttttcttatg
     7681 ttacttataa gtagttttac atgctttttg actttaaaaa tacatgtgat gatacatgtt
     7741 atatgaatta tcaatgtacc cagtaatatg ctattttcat tatgtaaaaa ataacagggg
     7801 gtgtgtagat ggagattgat gaaacttgtt tgagtaactc ttataaaagt atgaatgact
     7861 tttttgagga aatggaactg gattcagaat tgctctatca attttatctt gcttttcgag
     7921 ggcaacagat ttcttttcca atgaaaatgt acgatcgtga gttagttaga aaaagaatcg
     7981 aaaacatgat agaacaggaa aaaactgtag atatccgtca attgacagaa gtttatggtt
     8041 tctctgcaag atggattcaa aaagtaatta aacaaaaaga aaggagacga gtacatggct
     8101 aagctgattg tattaggtgg tttaatttac ggtggatatt ggttatataa atatatgctt
     8161 gcgcatccga atatagataa aggaaaagta attaagacag ttggggtaat catagttgga
     8221 ctcatagctc taactaagaa aaataatact aatagcccaa aaataatgag aacttatcgc
     8281 tgtagaggat gtggaaggga aatttctact agttttaatc catctgaaag ccctcaagga
     8341 agagatcgtt gtcatgctgg ctatcgacat tcttgggaaa ggattaacta aatgaacacc
     8401 attgagatta attgactgat aaaataagta cgcgagggag aaattgaaag ggggaaataa
     8461 aatgaaaaaa tggttgttat tactaattgg aatggggatt ttattcactg gttgtacatc
     8521 tgagaatacg aggactaaag agactgtaga aactgataca acaattgagg ctaaaaaaag
     8581 taaaacgcaa tcaactgaac aaactacaac agaaatgaaa caatctgtgg aagcaagtac
     8641 aagtgaaagt tcaatcattg aatcttcttc tgaagaggtt aaaaatactt tgtgggatag
     8701 tgagaaatct actcaactag caatttttgt cccacaatgg ggaaagacat taggtcaaga
     8761 atataaaagc tacaatcctc agaacaatgt aagtctgtat ggtacgcctt taccaagtgc
     8821 agttattgat ggaaattgga aaatggcaat caatgaagca ccagttaccg tacaatggtc
     8881 agaagatgga actggtcatg cagatttcaa tttagtagca gtttattctg atgtagaaac
     8941 tggagaatat ttagggcagc atgtatattt ctttggcttt caaaatggac aacctaaagt
     9001 ttatttaacg caacaaaatc aaggcaatga taaaaactac atgtacttca atgaaactca
     9061 gaaccaacag ttaaaacaag gtttcattga tattgtgaat ggacaaactc ctcaaactcc
     9121 agtagtagaa gaatctactc aacaagcaac aactacaacg acacaatcta caagtagctc
     9181 agcaatttca agttgggaag ctgctaaaga aactgtacta aataataaag ctttatggtt
     9241 gtatgattct cctgataaaa atgcatcaaa tactaattta attagcacaa atgaatatat
     9301 gaaagatcta atgcaagatg aaaatggaag ctactattca attaatattg taacggatga
     9361 taatggaaat acaacaatgg ctggtggaaa acaattccgt gtttatacag atggaagaat
     9421 ttctcaacgt attggaatga ctaacaattt tcaaatgatt aaataattga aaat
//
LOCUS       DAYYCM010000090         9300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000090 DAYYCM010000000
VERSION     DAYYCM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.094"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..699)
                     /locus_tag="ACHAY1_002524"
     CDS             complement(31..699)
                     /locus_tag="ACHAY1_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8594475.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            833..1120
                     /locus_tag="ACHAY1_002525"
     CDS             833..1120
                     /locus_tag="ACHAY1_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594476.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1536..3074
                     /locus_tag="ACHAY1_002526"
     CDS             1536..3074
                     /locus_tag="ACHAY1_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8594477.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3071..4036
                     /locus_tag="ACHAY1_002527"
     CDS             3071..4036
                     /locus_tag="ACHAY1_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594478.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4023..5345
                     /locus_tag="ACHAY1_002528"
     CDS             4023..5345
                     /locus_tag="ACHAY1_002528"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8594479.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5408..5512
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5586..6329
                     /locus_tag="ACHAY1_002529"
     CDS             5586..6329
                     /locus_tag="ACHAY1_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594480.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6344..7336
                     /locus_tag="ACHAY1_002530"
     CDS             6344..7336
                     /locus_tag="ACHAY1_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8594481.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7348..8085
                     /locus_tag="ACHAY1_002531"
     CDS             7348..8085
                     /locus_tag="ACHAY1_002531"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594482.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8075..8797
                     /locus_tag="ACHAY1_002532"
     CDS             8075..8797
                     /locus_tag="ACHAY1_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8594483.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8881..>9300)
                     /locus_tag="ACHAY1_002533"
     CDS             complement(8881..>9300)
                     /locus_tag="ACHAY1_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8594484.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3048 a         1603 c         1965 g         2684 t
ORIGIN      
        1 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
       61 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      121 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      181 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      241 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      301 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      361 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      421 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      481 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      541 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      601 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      661 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      721 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      781 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      841 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      901 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
      961 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1021 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1081 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1141 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1201 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1261 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1321 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1381 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1441 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1501 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1561 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1621 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1681 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1741 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1801 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1861 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1921 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     1981 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2041 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2101 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2161 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2221 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2281 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2341 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2401 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2461 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2521 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2581 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2641 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2701 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2761 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2821 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2881 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     2941 aattaagcgg aaaaataaca gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3001 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3061 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3121 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3181 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3241 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3301 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3361 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3421 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3481 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3541 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3601 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3661 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3721 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3781 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3841 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3901 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     3961 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4021 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4081 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4141 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4201 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4261 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4321 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4381 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4441 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4501 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4561 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4621 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4681 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4741 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4801 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4861 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4921 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     4981 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5041 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5101 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5161 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5221 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5281 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5341 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5401 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5461 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5521 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5581 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5641 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5701 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5761 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5821 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5881 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     5941 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6001 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6061 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6121 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6181 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6241 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6301 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6361 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6421 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6481 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6541 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6601 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6661 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6721 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6781 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6841 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6901 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     6961 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7021 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7081 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7141 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7201 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7261 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7321 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7381 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7441 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7501 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7561 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7621 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7681 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7741 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7801 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7861 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7921 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     7981 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8041 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8101 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8161 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8221 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8281 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8341 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8401 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8461 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8521 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8581 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8641 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8701 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8761 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8821 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8881 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     8941 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9001 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9061 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9121 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9181 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9241 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DAYYCM010000091         9088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000091 DAYYCM010000000
VERSION     DAYYCM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.095"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..801)
                     /locus_tag="ACHAY1_002534"
     CDS             complement(<1..801)
                     /locus_tag="ACHAY1_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594485.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFV"
     gene            complement(794..1423)
                     /locus_tag="ACHAY1_002535"
     CDS             complement(794..1423)
                     /locus_tag="ACHAY1_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8594486.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1416..2534)
                     /locus_tag="ACHAY1_002536"
     CDS             complement(1416..2534)
                     /locus_tag="ACHAY1_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8594487.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2536..3168)
                     /locus_tag="ACHAY1_002537"
     CDS             complement(2536..3168)
                     /locus_tag="ACHAY1_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8594488.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(3179..4996)
                     /locus_tag="ACHAY1_002538"
     CDS             complement(3179..4996)
                     /locus_tag="ACHAY1_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8594489.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(5009..5773)
                     /locus_tag="ACHAY1_002539"
     CDS             complement(5009..5773)
                     /locus_tag="ACHAY1_002539"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8594490.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5801..6499)
                     /locus_tag="ACHAY1_002540"
     CDS             complement(5801..6499)
                     /locus_tag="ACHAY1_002540"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8594491.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6511..7290)
                     /locus_tag="ACHAY1_002541"
     CDS             complement(6511..7290)
                     /locus_tag="ACHAY1_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8594492.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7306..8250)
                     /locus_tag="ACHAY1_002542"
     CDS             complement(7306..8250)
                     /locus_tag="ACHAY1_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8594493.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8296..9075)
                     /locus_tag="ACHAY1_002543"
     CDS             complement(8296..9075)
                     /locus_tag="ACHAY1_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594494.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2468 a         1864 c         1519 g         3237 t
ORIGIN      
        1 aacaaattca atatgatttt tttcacaaat atcagctaat ttagttttgc tttcaccttt
       61 tccaataaag taaagattta ttttgtttcc aaattttctt ttgcatgttt tcaataattc
      121 attaatcgct tctaaatcta cgatattatt aatcgtacct aaataagcaa ggttcaacgt
      181 agactctaag cttttattga ctattttctc tccataatca ttttggtcat ctacattgaa
      241 agaacatgga taaatggtca tattttttga ttctccggaa atttttatga attttttgaa
      301 ataatcgcat tctgttataa ctagactaga attactgaga tattgatctc gtagcttttt
      361 ccataaatca gtaaaaaatt tatatttgtt ccctattccc ggcattgact ctggccataa
      421 atcatacaca tcaaaaataa ctcgattgca tctactaaac ttagcaataa aaaaaactaa
      481 ggtattaggt ggtattctac aataaataat ttcaggcttt tcttttcgaa taaataataa
      541 tgtttttatt gaaaaccata tatgagataa aattcgcatt acggatatat tttttttata
      601 tccgatcgtt tttatcacat gacgattttt aatcatatgc ctttctttac tggatagaaa
      661 ttgttttctt atatgtgagt aatcagatac aacattaaaa tattcgatac tgttttccgt
      721 cagaatttcc tctaatatat tcgttcgact gatataatca aaaggatgaa taagcagcca
      781 ctttttttta ttctcactca tctatcttcg caccctcaaa tttctcagta gtcccacttg
      841 ctactgatgt tattccatca cggacaataa ctttctttag cgttaaaaaa aatattttta
      901 tgtctttcat cattgaaatg ttctccacat attcaatatc tttttcaaat ttttctttcc
      961 aactaattgc attacgacca ctgacttgag ctaatccggt taaacctggg cgcacaaaat
     1021 gtctttttcg ttgttcatta ctgtatagtg aaagatactc aactaacaga ggacgcggtc
     1081 caataatact catatctcct tttaaaatat tccataattc tggtagttca tcaagactag
     1141 tcgaacgcaa tattttacca aattgagtca atctgatatc gtctgataat aaattacctt
     1201 cttcatctct ttgatctgtc attgttcgaa atttatacat cagaaaaatc ttttcatgtt
     1261 ttccaggtcg ttgttgttta aagatcactg gcgatccaag ctttattcga actaggattg
     1321 caacaaacgt catcagtggc cataacatta ttaaacctaa aattgaaaaa agaatatcta
     1381 tcaaacgttt aaagaacttt tcgtagatac ctctattaga cattttgcat cactcgctta
     1441 attgtattag ttactcgata caaatcatca tcggtcattt tagaatctga aggtaaacag
     1501 ataccttttt caaacaatgt ctgagcaaca tctgtaccaa tatactcata cttatcgaaa
     1561 actggctgta aatgcattgg tttccatatc gggcgggctt ctatattatt tttatctaac
     1621 tcatcaataa gatgattcgt atttattccc tttactgtta tgacacttaa ccaataattt
     1681 ggttcattcc aattattttc tggcatgaat tgtatatttg acaatttaga caattctttt
     1741 ttataaaaac tatgaatata tcgttttttc tcaattcttt catttaaaac tttcaattgt
     1801 cctcgaccga tacctgcaac gatattgctc attcgataat tatagccaat ttcattgtgt
     1861 tgataatggc gtgccttctc acgagattgc gtagcccaaa acctcacttt ttccaccttt
     1921 tctttgtcat tagacacaag cattccgcca cctgaagtcg tgattatctt atttccatta
     1981 aaagaataaa ttccatagtc gccgaatgta ccagtatatt ttcctttata tgttgttcct
     2041 aatgattctg cagcatcttc aattaaaaca acattatgtt ctttgcatat tttagtaatt
     2101 tcatctagtt cagctgataa accgtacaaa tgcactacta atacagcttt tgcgttagga
     2161 tatttttcta gcgctgttcg caaatgttca gggttcatat tccaagtttc gtaatcacta
     2221 tcaacaaata ctggaatagc tccttgatag atgattggat tggcagtagc tgagaacgtc
     2281 aaagactgac aaatcactat atcatcttta ccgaccccag ccgcttttaa agctagatgt
     2341 atagctgctg ttccagaaga tagtgcagct gcatgactag caccgacata ttctgctaat
     2401 tcctcttcaa aaccattgac attcataccc aaaggagcca cccaatttgt atcaaatgct
     2461 tcttttacat actcttgttc atatccttca tcactcatat gaggagatgc taataatatt
     2521 cgtttatttt ccaacttaat cccatctttc tatgactttg gtaggtacgc caacattttt
     2581 agtcttatta ttaatatttt ttattactac acttcccgca cctataatgt tcatatttcc
     2641 aattcttaga ttttgtatta ttttagtacc tactccaatt tcatttttat ctcctaatag
     2701 aacatgtcct gaaatattta cacttggaaa aattgaatta taattaccga tttgagtatc
     2761 atgtccgata gtagagccga tattgatcat attgaaattt cctaaaacta cgtcagcagt
     2821 gtaagtcgta tagggcatca aaatattgcc aattcctaac tgaacattta tgcccactag
     2881 tgcacttgat gcaactaaat tagggaaaat caatttatta tttttattta tcttttgata
     2941 tattttctct ttgatatctg gcgaagcgat tcccagaaaa atagcagttt cttcttctaa
     3001 ttctaataac gagtctattg ttcccaatac agtttttccc actaattctt gattttgcat
     3061 ttttaacgaa tcatcgacaa aaccaatcaa ttcatatcta gagaaacttt ctagtagaaa
     3121 agcaatctct ttgccaaatc caccagctcc tacaataatt gctttcactt tactccccct
     3181 acttattcga agcattcgca aaagtgacaa tgtctttagc caattgttca tcgtcttgcg
     3241 gcaggctctt gacaaattcc atcactgttt gcatggaata acctttgatg tttcccacaa
     3301 agatcttctc gtaaactgct tcatcgtttc tttccttatc taacaatagt tcttcatata
     3361 gtttttcccc tggacggata cctgtttcaa tgatttcgat ctcgtcttcg ctatatccgc
     3421 tcaatcggat catgtttttt gctagatcta cgattttcac tggttcactc atatctaaaa
     3481 taaagatttc gccgcctttt gctaacgccc cagattgaat aactaaacga cttgcttcag
     3541 gaatcgtcat gaaatatcgg gtcatacgaa agtctgttac agtgactggc cctccttgag
     3601 caatttgttc ccggaatact ggaatcacac taccgcgaga accaaggaca ttcccaaatc
     3661 tgacagctga gaatttcgta catccttctt cattcaaccc agtcacgatc atttcagcga
     3721 ttcgttttgt tgaacccatg acgtttggcg gattgttggc tttatctgtc gagaccatga
     3781 cgaagttttt tactttcgct gctttggctg cttctgctac attttttgta ccgaaaatat
     3841 tgtttttgac tgcttctttt gggttatatt ccatcaatgg tacatgctta tgagcggctg
     3901 catggtagac gatatctggg tggtattgtt ccatgatctc attgattttc ttgcgatctt
     3961 ggatatccgc aatgatcggc acgatttctg taggacactt ttgatgatgc gtgcgtaatt
     4021 cacggtcaat caagtaaatc gagttctccc catgacctaa aagtaatagt tttgcagggt
     4081 caaattggat gatttgccgg cagatttctg aaccgattga tcctcctgca ccggtgacaa
     4141 gaatgacttt gtttgtgatc tggtctttga tggattcgat atccaactct acctcatctc
     4201 ttcccagtaa ttccactacg tcgatggttt ttaattttga tacgctgatt ttccctgagg
     4261 caagttcttc catagaaggc atcgtattga ctttcacatg agcagattcg actaactcga
     4321 agattcttcg taattttttt cttgataaag aagggatagc gatcgttacc atttggatgt
     4381 catatttttc gatcaattcc ggtatatctt ttaatgaccc cagtactttt tttcctgaaa
     4441 gataggtatt tcttttattc ggatcatcat ccacgaatcc aatgacatgg atatcttgag
     4501 ccgttttaga accaagaaag ctgttgtata aaatacgtcc accttcgccg gccccaacga
     4561 tcaaggtatt tttagcggaa tctgttgata gatacctcat attttttgtt tcgatataga
     4621 tacgccagat caatcggctg ccaatgatca gtaatagcga aagtaggtat gcaaagatca
     4681 ctaaacgtaa actgtattgt ttatttatga aaagcaatat gatgatactt gaggccgcac
     4741 ttgcagataa cgaaccaaaa atcgcaatga tctctcttaa atttgtataa cgattgattc
     4801 gagtaaaaac tttgaataaa ctaccataga ataaataaaa accaatcgac aaaccgatag
     4861 agattaaaat aaggtccatt ggtatagcta caaacggctt catgaattta gctgctgcaa
     4921 tattcgcagc aatcaacaac aagctgtcca caaataccaa tgtggcaatt tttcttcttc
     4981 ttgttaatac aaacacccta cattccccct aaaataaacc aaacttcttc ttttttacct
     5041 cttcatagac tggataactg atctcatccc cgttgatcag gtcctttgcg acttgtttca
     5101 tctgtgctac tttttcctta ccaaagtcct tggcgatttg ctcataacat tcttttaagt
     5161 aaaaggtacg tttagagacc ccatgggcat cggaagcagc catttgcacg aggttatgtt
     5221 tgaccatcat tttggctgtt ttctggatgt cttttccaaa tacaccgaca taacttggtg
     5281 cggtgagttg agccaagcat cccatatcta ggaatggcag caaatgattt gggtcttctc
     5341 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tgtaacaact
     5401 caaaaaagag ccattctgca tataacggca cttccagtgt cggaaattcg attagaagat
     5461 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttca atcagtgttc
     5521 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caattgtctt ttctctaatt
     5581 ctgcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     5641 tattgtgatg gggtgtgcat aaaatatgtg tgatcccttg ttgaatcgct ttttcagcca
     5701 tggcgatact ggcatctatg ttttctgctc cgtcatcgat tcctggcagg atatggcaat
     5761 gtagatcgat catttctctt gtctcctctc tttttatttc ttaattcccg tagtaatagc
     5821 cagcatctct gctgtgttct gctccattgt atacaacacc aaggactcgc gcttgtacca
     5881 tatctaacag ttcttttgct ttagtcaatg attcttttcg tgacgtattt tcccgtacga
     5941 ccaataaggt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gcaacgactg
     6001 gtggcatgtc gaaaatcact acatcaaata gttgtcttgc ttctgctaaa atctgatcca
     6061 ttcttggcga acttagtagt tctgatggat ttggtggttt aggtccactt ggcagaatcg
     6121 acaaattgtc gactggtgtt cgttggatct catctgctac acttcctgat gaactaagaa
     6181 cagtacttaa tccgcttgcg ttgttcaact tgaacgtttt atagatgacg ggcttacgta
     6241 agtctgcatc gactagcagt acccgttgac cagatttggc aaaaacaacg gcaatgtttg
     6301 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     6361 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     6421 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttga gttgcttttt
     6481 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     6541 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gatccttttt tacggatacc
     6601 agcgttcaat tcttttgctg acatttctgg aactgttcct aagattggga atcctaatgt
     6661 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     6721 acctacgcct accatcattc ctaacactaa gccaatcact agattcaatt tattatttgg
     6781 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     6841 cacgtctttg gcgttttctt ggaaaacttg tgaagttgta ttagcgatct gttgagcagt
     6901 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     6961 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     7021 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     7081 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     7141 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     7201 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     7261 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
     7321 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc tgactttttg
     7381 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct tgatcgtacc
     7441 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     7501 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     7561 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     7621 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
     7681 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     7741 accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga catagtgatc
     7801 gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc cgccgaatgc
     7861 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
     7921 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
     7981 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca tcaatagaac
     8041 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     8101 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     8161 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     8221 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     8281 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     8341 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     8401 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     8461 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctagata
     8521 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     8581 agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag cttgctgcca
     8641 gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca gttttgattc
     8701 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     8761 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag aggatagctt
     8821 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat ttttttcttt
     8881 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     8941 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     9001 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     9061 gaatggcttt tccatagatt cactccta
//
LOCUS       DAYYCM010000092         9050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000092 DAYYCM010000000
VERSION     DAYYCM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.096"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHAY1_002544"
     tRNA            2..74
                     /locus_tag="ACHAY1_002544"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHAY1_002545"
     tRNA            78..150
                     /locus_tag="ACHAY1_002545"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHAY1_002546"
     tRNA            155..225
                     /locus_tag="ACHAY1_002546"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHAY1_002547"
     tRNA            238..311
                     /locus_tag="ACHAY1_002547"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHAY1_002548"
     tRNA            323..394
                     /locus_tag="ACHAY1_002548"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHAY1_002549"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHAY1_002549"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8594495.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHAY1_002550"
     CDS             1983..3455
                     /locus_tag="ACHAY1_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8594496.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHAY1_002551"
     CDS             3538..4326
                     /locus_tag="ACHAY1_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8594497.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHAY1_002552"
     CDS             complement(4393..5061)
                     /locus_tag="ACHAY1_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8594498.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHAY1_002553"
     CDS             5176..6888
                     /locus_tag="ACHAY1_002553"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594499.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACHAY1_002554"
     CDS             6891..8663
                     /locus_tag="ACHAY1_002554"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594500.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9050)
                     /locus_tag="ACHAY1_002555"
     CDS             complement(8745..>9050)
                     /locus_tag="ACHAY1_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594501.1"
                     /translation="KLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSS
                     HMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2715 a         1523 c         1996 g         2816 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt
//
LOCUS       DAYYCM010000093         8970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000093 DAYYCM010000000
VERSION     DAYYCM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.097"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            312..908
                     /locus_tag="ACHAY1_002556"
     CDS             312..908
                     /locus_tag="ACHAY1_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8594502.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            889..1335
                     /locus_tag="ACHAY1_002557"
     CDS             889..1335
                     /locus_tag="ACHAY1_002557"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8594503.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1678..2001
                     /gene="relB"
                     /locus_tag="ACHAY1_002558"
     CDS             1678..2001
                     /gene="relB"
                     /locus_tag="ACHAY1_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8594504.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2321..2548
                     /locus_tag="ACHAY1_002559"
     CDS             2321..2548
                     /locus_tag="ACHAY1_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8594505.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2564..3730
                     /gene="hemW"
                     /locus_tag="ACHAY1_002560"
     CDS             2564..3730
                     /gene="hemW"
                     /locus_tag="ACHAY1_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8594506.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3965..5008
                     /gene="hrcA"
                     /locus_tag="ACHAY1_002561"
     CDS             3965..5008
                     /gene="hrcA"
                     /locus_tag="ACHAY1_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8594507.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5102..5665
                     /gene="grpE"
                     /locus_tag="ACHAY1_002562"
     CDS             5102..5665
                     /gene="grpE"
                     /locus_tag="ACHAY1_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8594508.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5716..7545
                     /gene="dnaK"
                     /locus_tag="ACHAY1_002563"
     CDS             5716..7545
                     /gene="dnaK"
                     /locus_tag="ACHAY1_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8594509.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7696..8862
                     /gene="dnaJ"
                     /locus_tag="ACHAY1_002564"
     CDS             7696..8862
                     /gene="dnaJ"
                     /locus_tag="ACHAY1_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8594510.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3143 a         1448 c         1913 g         2466 t
ORIGIN      
        1 attcaatatg tagcgcttac taaattattc gccataaaat tttataattg gatcactcat
       61 tgttttacct aagtcatatt taagtaataa ccggtgtttt tacataagaa gacttcgggt
      121 attttttagt ttgattagct ctgatatacc tataaagaat atgctaaggt aagaatagta
      181 aaaattcaca tagatgaggt ataataatga tatttttata tattacagat aacttgacac
      241 aaaatgagtt tttttcgatt tttcaagtta tctaatgcaa ttatattgat atcattagga
      301 gctgcattgt tatgaatcca acattgatta ctaagaaaga attactaaaa aaattagata
      361 tttctactgg tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt
      421 ttaatcttga taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt
      481 tagaagttgg tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac
      541 agggtggaat gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag
      601 gaagtaatgt ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc
      661 taaaagggga tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta
      721 attttgtaaa gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag
      781 aagtatattt agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca
      841 gaaaagtata taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag
      901 atatataacc aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta
      961 gaggaagaag caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac
     1021 gaagaggagt tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa
     1081 tatgttaaac gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata
     1141 aaagacggtg taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa
     1201 gatgtgattg aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt
     1261 gagttacctg acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt
     1321 tctaaaatac cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta
     1381 tataagacta agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat
     1441 tttttaaata acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg
     1501 aggattcttt ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat
     1561 ttctgaactt ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc
     1621 ctacattttc acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg
     1681 agattcttta agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa
     1741 aaagaatggc tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa
     1801 aactattcga tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat
     1861 aaacattgga taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg
     1921 gtgggctgga atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt
     1981 ggcaaatacg tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag
     2041 tagggcaccg aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa
     2101 ttggaatcta gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa
     2161 taaggacaaa attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt
     2221 cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg
     2281 taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat
     2341 ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc
     2401 tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct
     2461 tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt
     2521 cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa
     2581 tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta
     2641 ttactgtgac tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat
     2701 gctgctgaat gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact
     2761 ttatgtagga ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc
     2821 aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc
     2881 tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc
     2941 catgggagtg cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc
     3001 agaagatgtg tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat
     3061 cgatttgatt tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa
     3121 agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac
     3181 aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac
     3241 cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat
     3301 aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga
     3361 ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa
     3421 caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga
     3481 agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa
     3541 aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt
     3601 gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg
     3661 tgtttatttg acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt
     3721 tgaaaaatag tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa
     3781 agtttgacaa tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat
     3841 tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct
     3901 tcttgctata tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag
     3961 gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag
     4021 tagtggcgtg cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc
     4081 aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc
     4141 ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa
     4201 acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt
     4261 tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta
     4321 tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt
     4381 acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag
     4441 ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat
     4501 tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga
     4561 gattccgatg attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga
     4621 caccgttctt ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt
     4681 ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga
     4741 ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg
     4801 tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat
     4861 agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg
     4921 gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta
     4981 ccgctcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt
     5041 tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat
     5101 cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga
     5161 aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga
     5221 aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc
     5281 tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg
     5341 ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc
     5401 cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga
     5461 aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc
     5521 ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc
     5581 cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc
     5641 aactatggtc attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag
     5701 aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac taactctgca
     5761 gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca
     5821 acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt
     5881 caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt
     5941 tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt
     6001 caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc
     6061 actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc
     6121 gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt
     6181 ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt
     6241 gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac
     6301 aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc
     6361 aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac
     6421 gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca
     6481 tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa
     6541 tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta
     6601 aaagatgcag gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact
     6661 cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca
     6721 gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt
     6781 gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg
     6841 ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa
     6901 gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa
     6961 cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca
     7021 gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta
     7081 aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct
     7141 tcaggtctaa cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa
     7201 gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc
     7261 tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa
     7321 gcagaaacag ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg
     7381 aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa
     7441 caagctgcac aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat
     7501 gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt
     7561 tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc
     7621 ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca
     7681 ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa
     7741 ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct
     7801 gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag
     7861 attttaagtg acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat
     7921 ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt
     7981 ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca
     8041 gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt
     8101 gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat
     8161 acttgtggtg gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat
     8221 ggtgcaggta cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa
     8281 acttgtgatg tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat
     8341 ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa
     8401 gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt
     8461 ggtgatttgt atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct
     8521 gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa
     8581 gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg
     8641 aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa
     8701 catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta
     8761 cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc
     8821 gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat
     8881 aaagtagact tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa
     8941 aaccagacga acaatcatac gttcgcctgg
//
LOCUS       DAYYCM010000094         8963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000094 DAYYCM010000000
VERSION     DAYYCM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.098"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..80
                     /locus_tag="ACHAY1_002565"
     tRNA            7..80
                     /locus_tag="ACHAY1_002565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Ile,seq:cat)
     gene            complement(465..962)
                     /locus_tag="ACHAY1_002566"
     CDS             complement(465..962)
                     /locus_tag="ACHAY1_002566"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8594511.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1002..1811)
                     /locus_tag="ACHAY1_002567"
     CDS             complement(1002..1811)
                     /locus_tag="ACHAY1_002567"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8594512.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2145..3002
                     /locus_tag="ACHAY1_002568"
     CDS             2145..3002
                     /locus_tag="ACHAY1_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8594513.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2995..3897
                     /locus_tag="ACHAY1_002569"
     CDS             2995..3897
                     /locus_tag="ACHAY1_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8594514.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3930..4280
                     /locus_tag="ACHAY1_002570"
     CDS             3930..4280
                     /locus_tag="ACHAY1_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8594515.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4329..5252
                     /locus_tag="ACHAY1_002571"
     CDS             4329..5252
                     /locus_tag="ACHAY1_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8594516.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5330..6223
                     /locus_tag="ACHAY1_002572"
     CDS             5330..6223
                     /locus_tag="ACHAY1_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8594517.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6261..6824
                     /gene="amaP"
                     /locus_tag="ACHAY1_002573"
     CDS             6261..6824
                     /gene="amaP"
                     /locus_tag="ACHAY1_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8594518.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6838..7026
                     /locus_tag="ACHAY1_002574"
     CDS             6838..7026
                     /locus_tag="ACHAY1_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8594519.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7039..7941
                     /locus_tag="ACHAY1_002575"
     CDS             7039..7941
                     /locus_tag="ACHAY1_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8594520.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7973..8215
                     /locus_tag="ACHAY1_002576"
     CDS             7973..8215
                     /locus_tag="ACHAY1_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8594521.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8471..8842)
                     /locus_tag="ACHAY1_002577"
     CDS             complement(8471..8842)
                     /locus_tag="ACHAY1_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594522.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3114 a         1552 c         1741 g         2556 t
ORIGIN      
        1 tatgatgtac ctttagctca gttggttaga gcagacggct cataaccgtc cggtcgtagg
       61 ttcgagtcct acaaggtaca taagaggttg tgaaaaagct gccctgcatc aagtaataag
      121 aacagccaag taaaaacagc ttcttatgtt tttacttggc tgttcttatt actgcagatg
      181 caagcttttg aataccgttt aaggaagagg ttgtgaaatc agcgttttga cctgagtatt
      241 agcttaacaa acggaaaaat agttcctcat attttgccgt ttgttaagct aataccgagg
      301 gtcagctgat tgaacaccat ttaagaaaga ggtcgtgagc ctgccgtttc actctgtgtc
      361 ataaaaaagc caatctactt tgatagaaaa agcacgtagg actaaaacag ttctgcgtgc
      421 tttttttatt gtgtgttaaa tatcgtaaat gaagatagat atttttatga ttcgacgata
      481 gagacgttga aatctttcaa cagctctaaa gcggattctc cgtgttttct actggagact
      541 aacattggat caacaaaaaa gtctgcttca ggaaaagcac tctgcaggcc tatcagatta
      601 gagagtacac acaagtttgt ttcaatcccg actacttcga tctctttttg ttcttctttg
      661 ctttcaaata aagtttcctg gatttcagct aaagtttcag gtggaatcgt aaagtaatac
      721 tttttaatca ctcgttcatt ttcttgtgga gaaagaagag ggatcaattg caaatcttct
      781 cgttcttctt ctttgtcctt cagttcaact ggtatatccc gtgtgaagag aatatattca
      841 ttattttggc gtgctttagc taatctttga gcaatacgtt ctgccaattg atctgactct
      901 ggaatataaa attcgctgtt tggatcaaga atatgatttt gcatatcaat aataactaac
      961 attttctttc ctcctacatt catatcgtca ttttattctt tttaatagaa gttttttaaa
     1021 atataagcaa caccagcttc ttcattcgtc agtgttactt ggtttgcaat ttcttttact
     1081 tgtcgctgtg cattccccat cgcaatactc aacccgctgt aattcaacat agcaatgtcg
     1141 ttttcttgat cgccaaaagc catgatgtca ctcgattcga ttccaaggta atccgccaga
     1201 aaaacaagtg aatgagcttt agtgatacct gtaggcgtga tttctaagaa atgcggatca
     1261 gagaatactg attggatacc atctgcttgc agatctcttt gtagcttctg tagtttttct
     1321 tcactcttat ctgttaatgc taatttaaga tagctaagag aaggatcttc caacatattt
     1381 aaaatttgat caaaagaatg gttctgtaaa ttgaaatgtt cataataagg gtcatctttg
     1441 actagatttg attcatcgaa tgtcagctga tcatcttggg tttctcggct agcaatgatg
     1501 gatattttcg cttcttgagc atagaagaag gcttttctca gtgtatcgct atctattttt
     1561 gaacgtctga gaactttcgc catttttcca tccttcattt cagcaatcag tgaaccattg
     1621 gaagaaatca aatagccacc gattcctaat tcggaaaaaa tatctttagc agaaatgctg
     1681 ttccttccgg tagcgacggt tacgattccg ccattttcat caaatttccg aagtgcttgg
     1741 atattttctt ctggtatctt tccatttcga tcaagcaagg taccgtctaa atcaatcgct
     1801 gctagtttca ttttatcttc ctactttctt tttacatatc tattttcctg ttaccttaag
     1861 attaaatgag aatttaaaaa aatgaaacta acttgctttg tcattatgaa ttttttcaaa
     1921 aaacattctt aagaaaaagg tggctatata tagtagtttt tggtcaatac ctttgaataa
     1981 aaagccatga aaaacgaata gttagcgctt accgatgcgt tttgtgataa aaaaatttta
     2041 aaatattcaa tgaaaacaaa gcatatattt tgctttaaaa ccaaatgagg aatattctag
     2101 atgtagaaca ggttagaaaa agattagaaa aaggagtgat tttgatggag aagagatacg
     2161 gcgtagttcc tgtggtaact gcttcagacg aaaattatgc tccttacttg agcgtgatga
     2221 tcgcaactgc attagaaaat tgcaataaaa caagacgcat aaaattttat gtgattgacg
     2281 atggtctatc agaatatagt aaagaaggat tggaagaaac ggtaaataaa tattcaagta
     2341 atgcaagtat ccaattcctg acagtggaaa aagatatcta cgaagacttc ctagtcagtg
     2401 accatatcac aactaccgct tacttaagaa tttctttacc aaacttatta gctaaagagg
     2461 attataagaa agtcctttac ttagattcag atgtgttagt actagacgat atcgtaaaac
     2521 tatatgatga gccgttaaat ggaaaaacaa tcggcgctat tatcgatcca ggccaagtga
     2581 aagctttgga aagattagga atcgattccg atgacttgta cttcaattca ggagtgatgg
     2641 tgattgacat tgatcaatgg aataaaaaag aaattacaga aaaaactatc cattacctca
     2701 gtgaaaatgg cgatcgaatc atctatcacg accaagatgc tttgaatgct gtactttatg
     2761 aagattggga acaacttcat ccaaaatgga acatgcagac ttcgctgatt tttgaacgtc
     2821 atccagcacc aaatgaaaaa tatgaacgtc agtataaaga aggaaatgaa aaaccttcga
     2881 tcgttcactt tacaggacat gataaacctt ggaatacgtt gaaagatcat ccgtatacca
     2941 atttatactt aaaaaaatta gcacacagta ctctaacgaa agtaggcgag atcaatgaat
     3001 aaaaaagaaa tagcagtagt cgcaagttgt aacacaaaat tcgtaccaca tttagctgca
     3061 ttatttgtct cagtattaga taattgcaac ccgtctaaat ttgttcggtt ctatgtgatt
     3121 gacgatgata tcgattttga aagtaaacag cttttacgct tctcagtgaa aaacgcacga
     3181 atgaactcag atgtggaatt cttgaaaatc aataaagaat tctttacaaa tgtggtaatc
     3241 agcgatcgta ttccagaaac agcttattat cgtattgcga ttccggaatt gttccgcggc
     3301 acagaagttg aacgtatctt atatatggat tgcgacatga tcgctttaca agatatctct
     3361 aaattatggc gtcttgactt cggtgattca atcgtagcag ctgttgaaga cgctgggttc
     3421 catcaacgac tggaaaaaat gaaaatttca gctaaatcaa tgcgttattt caactcaggt
     3481 ttgatgctga tcaatgtgaa aaaatggtta gatgaaaata ttacgcaaaa agtattagat
     3541 ttcatcgaac ataatccaga aaaattacga ttccatgacc aagatgcatt aaatgctatc
     3601 ttacatgacc gttggttacc acttcatccg cgttggaatg cacaaggcta tatcatggca
     3661 aaagcaaaaa aacatccaac agcagcaggt gaacgtgaat acgaagaaac tcgcaacaat
     3721 ccttacatta tccatttttc aggacatgtg aaaccgtgga gcaaggattt tgaagggcca
     3781 acgaagaaat attacgaaaa atatgcaggg atgacggctt tccgttgtgt agctaaattc
     3841 cctaaatatc caaaatatgc aaaagttcaa ccaaaaacag atgtagttgt taaataatag
     3901 aagaccacaa ctgaagaggg ggagtaggaa tggaaattac aatgtctaaa aatgcagtag
     3961 agacgttaat tgaaaaggtt ggggaaaata caaaaatcgc actcgcgctt atcaatgatt
     4021 ccgatccatt tctaagggac aaaggtgctt ttgcaaaagg cagctttttt caaatcattc
     4081 cttttgtttc ggaatttgga gaatatgcga ctaaaatcga gcatccatta ttggacatct
     4141 atacatctaa attagagcaa aattatttcg gtaaacgatt gaacatggac ttcaacaaac
     4201 agcttgacag cttttcatta gaaaatgaaa tcgcagtact cgattataat atcaaattaa
     4261 agaactgctt cttcagctaa tagtttcgag aaagtccaaa aaggaggtat acattgagat
     4321 actactttgt ggatgaagaa aagaatgtaa tagaaacaga tgaaaaaaaa tataattggc
     4381 tcgtacttga tagtgcaaag cccgaagaga ttgagcaagc aatcgaagaa ttcgagcttc
     4441 ctgatgatat tttcgtagga accgaatatc cggaagaagt ttctcggatg gagcgccttt
     4501 atgagacgaa actagagcat ccattttctt tggtagtgat taatctagat cagtcagata
     4561 atcgtatcga aaaaaaatta acgccaatat cttttgtact ttccgataat ctcttgatta
     4621 cttgtatcga tagaaaaacc gaatttgtgg atcggctgct gacaaagcat ggaaatcatt
     4681 taaactcgtt tgaaaagatc atcacgtata cattattaga tatttacacc cattacgtga
     4741 aagaactaag agagatgaaa aagcgaattg acgctttgga taaagcagct cgtaaaacaa
     4801 cggaaaatga agaactgtat aaacaggcag atcttgaaag agacatcgta tatatcgatc
     4861 ataccttaag agatcaacgg gaaacaatgg atcaattatg gaattctgaa actttcatca
     4921 aacgtttaga cgatgaacag ctactctatg atgtccattt gagacagcga caaacagaaa
     4981 aaatgatcga gatctatcga gatttattag agagtattgg tgatctattc aacgggatga
     5041 tggacaataa tctaaatcat atcatgaaat atctagattc agctgcatta atcatttccg
     5101 ttccggcgtt gttcgcaggg atttggggaa tgaatacagg aggattaccg ggtaaaagtt
     5161 ctgctctagg ctttatactc gtagtctttg gctctttgat agcagcagta gttttaggtt
     5221 accatttata ccggaaagat tatacaaatt aaagaatcta tttaaaacag gtatcaaaca
     5281 aagaaatttg ttttggcaat acaatttaaa aactaggagg aatacagcaa tgacagaacc
     5341 aaaattaatc gacccgcgaa acttgtacca tacggagaaa tttcctaaac aagatcagga
     5401 tacaccagct ttacagcaga atatgattcc tgttcctgat tgtggagaag aatcctatga
     5461 aggcaataat caactagaaa accgccgtgt actaatcact ggtggagatt cagggatcgg
     5521 tcgtgcagca gcgattgcat ttgctagaga aggggcagat atcgctcttc aattcttccc
     5581 aggagaagaa aaagatgcag aggaagtagc aaaatacgtg aaagatgcag gacgtaagat
     5641 tgtcttacta ccttttgatc ttcgtgacga acaagcacct aaagaaatca tagataaagc
     5701 cgtgagtgaa cttggcgggc tagatacgat ggtactaaac gcaggacagc aaatttcttg
     5761 tcagtctatt gctgagttac cacttgagca agtcaaagac acatttatga taaacatcat
     5821 cgctatgttt gctttagtga aagaagctgt tccgcatatc ccagcaggag gagcaatcgt
     5881 aacaacaact tctgttcaag cattcaatcc aagcgaacat ttattagatt acgcagcaac
     5941 aaaagcttca atcgctaact ttacgatcgg tttagcaaaa cagcttgcac ctaaaggaat
     6001 ccgtgtaaat ggtgtagcac ctggtccaat ttggacacca ttacaactag acgaaggaca
     6061 accttctgat tcgatccctg aatttggtca gcaaagttta ctagaacgtg caggtcagcc
     6121 aggagaatta gcaccagttt atgttttctt agcatcgaat aaagcaagtt atgttacagc
     6181 ccaaatttac ggagtaactg gtggagaagc gatcaactta taagaagttg atcgtttcac
     6241 ccctatgagg aggtgcgaaa atgaataaag gtgtaaaaac gatcggagtg atcggaacat
     6301 tacttttgct atctgtgctt ttgtttgcga tactattaag tgcaacctat ccattgcctt
     6361 ttcagctaga gaagtttcga ttcttttcca tcactaattc atacgtgcaa caatatgtat
     6421 tttggcttgc cgttgcattt gctattttga caattatcac gttacttgtt ttcctgtttt
     6481 atccaaaaac aatcggaaac tttctactga aacatggtga tggggagttg acgttggata
     6541 aaaaagcaat tgaaggaatg gttcgttctc acttgcatga agatgaattc atccattcac
     6601 caaaagtaaa tgtcaaagca acgaaaaaca agatcgccgt caatgtaaaa ggggagttga
     6661 aacgaacttc agcactcatt ggtaaaacag gaacattgat gaaagaaatc gaaaatgaag
     6721 tacaacacat attgggaaca aaagagccag tgaaagtatc ggtgaaatac acaggctttg
     6781 aagaaccagc taaacgaagc aatgaccgca aacgagtaga ataggggggg accaaaaatg
     6841 aaagaactat tttcgacata caagctccct atcatctttg gggtacttgg attgatatta
     6901 gccgttttat ggatcaccgt gggctttttc aagaccttat tgatcttgat catggcagcg
     6961 atcggcgtcg ctatcggttt ttatctgaaa caaacgaaaa tcttagatca ctattttaaa
     7021 tcttaggagg aaacatccat gaataacaat aacaaaaata ataacaataa aatgaacaac
     7081 caaaaaccag tgtctgcaca tccagaccca acgacaccaa accaatcaaa tggtgtgaac
     7141 gatgaaaaga acaaaacagc accagtatct gcacatccag atccagtagt ccctaacgtt
     7201 caacaaggag tagaagagaa gaaacatgat gcagaaaagc atgcacctca tcatccagga
     7261 actccagaat ctaaagttcc agaacaacat gatgcaatcg atccttctaa acctgtggaa
     7321 gagcaaaaac atccttctga ccatgcgaaa gcacaagaaa aacttagttc tgaccacaca
     7381 aacggacaag gaaaacttgg ttctgatcat acaaataacc aaggaaagca tgattctgaa
     7441 caggaagatt caatgcgcgg tgagctgaat tatgaagata aagttgtgca aaaaatcatc
     7501 ggaatcgcaa ttgaacaagt agacggttta ttatctgcaa acggtggttt cttctctaat
     7561 gtggcaggaa aacttgtgaa tacagataat gtaacggctg ggatcgaaac agaagtcggt
     7621 aaaaaacaag tagcagttga tatggatgtc attgtggaat atggcaaaga tatcgaaaaa
     7681 atctttgaag aaatgcaaga agtaatcggc aaagaagtaa aaaatatgac acatttagaa
     7741 gtgatcgaag tcaacgctaa cgtagtggac atcaaaacaa aagaagaatt tgaaaaagat
     7801 caagaaacgg tgcaagacaa agtaactgac gcagcgaaaa aaaccggtga atttgcttcg
     7861 aatcaaacag ataaagtaaa atcagcagct ggaagcggcg cacaaaaagt aaaagaaaat
     7921 acagaaccac gggttcagta ataaggaagt aaaacattag gaggaacgaa acatgggatt
     7981 tatttggtca cttatcgtcg gaggaattat cggagcaatt gcaggagcaa tcacgaataa
     8041 aggttcttcg atggggatta ttgcaaatgt cgtagcaggt ttagttggat cagcacttgg
     8101 tcaagcatta ttaggttctt ggggaccaag tattgcagga atggcaatcg tgccatcatt
     8161 gattggtgcc atcattttag tactaatcgt atcattcttt gctcgaaaac tgtaaaaaga
     8221 cttttaaaaa gaggtcgtga aaaagctgtt cttcatcaag taataagaac agccaaacaa
     8281 aaatagcttc tcatattttt catttggttg ggcatattat cgaagatgca agcttttgaa
     8341 cactgtttat acggaataag aggctgtgac aagacttttg tcacagcctc ttatttatgc
     8401 gggcaaattc ccaattagat ttttaaaagt ttttccttaa atgtgtcttt attgatacag
     8461 agaagcttct ttaataaaac aatgcctccc taaattttct tgagatagga aaggtgaaca
     8521 tacaaaataa tacgaccact ttgaaaaccc agatgatcca caaatggatc gtatgatact
     8581 gatgaagtaa aaagcaatgg atcagaaact caaagagatt gaaaaagaca aggaagaaat
     8641 aatatctctt ttttactctt gtctgtttta gccaataata gctcgtaaag taatctagac
     8701 aaacaaatag aagtaaacaa agtttcgtaa gagcagcaat cgtatcatgt tgctctgcta
     8761 gacgattgac gagcaggaaa aatgttggta atggaatata gatcacgagt aatgaaagaa
     8821 aaaagtaaac aataaatggc atactattcc tcctaattgt gtgagttcgc cctcatttta
     8881 aaataacgat atttgtttgt gaagcttttt cgtgtcgtaa caaatgttat ctaatgtatt
     8941 tttaaataaa taatcattat tat
//
LOCUS       DAYYCM010000095         8952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000095 DAYYCM010000000
VERSION     DAYYCM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.099"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..253)
                     /locus_tag="ACHAY1_002578"
     CDS             complement(<1..253)
                     /locus_tag="ACHAY1_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594523.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSH"
     gene            complement(550..2931)
                     /locus_tag="ACHAY1_002579"
     CDS             complement(550..2931)
                     /locus_tag="ACHAY1_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8594524.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2921..3568)
                     /gene="recU"
                     /locus_tag="ACHAY1_002580"
     CDS             complement(2921..3568)
                     /gene="recU"
                     /locus_tag="ACHAY1_002580"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8594525.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3632..4171
                     /locus_tag="ACHAY1_002581"
     CDS             3632..4171
                     /locus_tag="ACHAY1_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8594526.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4261..4680
                     /gene="gpsB"
                     /locus_tag="ACHAY1_002582"
     CDS             4261..4680
                     /gene="gpsB"
                     /locus_tag="ACHAY1_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8594527.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4702..5069
                     /gene="rnpB"
                     /locus_tag="ACHAY1_002583"
     ncRNA           4702..5069
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAY1_002583"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5278..6444
                     /locus_tag="ACHAY1_002584"
     CDS             5278..6444
                     /locus_tag="ACHAY1_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8594528.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6490..7986
                     /locus_tag="ACHAY1_002585"
     CDS             6490..7986
                     /locus_tag="ACHAY1_002585"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8594529.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8067..8750
                     /locus_tag="ACHAY1_002586"
     CDS             8067..8750
                     /locus_tag="ACHAY1_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8594530.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8759..>8952
                     /locus_tag="ACHAY1_002587"
     CDS             8759..>8952
                     /locus_tag="ACHAY1_002587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594531.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2843 a         1603 c         1879 g         2627 t
ORIGIN      
        1 catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt acagaaaaaa
       61 taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa caaaattttt
      121 cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga tggtgcagac
      181 aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct ttcaaaaaat
      241 tattcaaaac catatattgt attttttatt tgataataac aatgggatta ttaagaagtg
      301 gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag taaatggata
      361 aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc
      421 acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt
      481 tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt
      541 tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc
      601 agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg
      661 tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt
      721 ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct
      781 agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga
      841 tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta
      901 cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt
      961 agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga
     1021 agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt
     1081 atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc
     1141 gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc
     1201 attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt
     1261 ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa
     1321 gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata
     1381 ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt
     1441 actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa
     1501 agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga
     1561 taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac
     1621 aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata
     1681 tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc
     1741 acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc
     1801 gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat
     1861 ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg
     1921 ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt
     1981 tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc
     2041 atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata
     2101 ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt
     2161 cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc
     2221 aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga
     2281 agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa
     2341 gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt
     2401 gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg
     2461 tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat
     2521 gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat
     2581 cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc
     2641 taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa
     2701 ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc
     2761 taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt
     2821 tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt
     2881 aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt
     2941 ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg
     3001 gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt
     3061 tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa
     3121 aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc
     3181 atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt
     3241 tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg
     3301 tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt
     3361 ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa
     3421 gataatattt attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt
     3481 cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag
     3541 gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca
     3601 aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc
     3661 tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt
     3721 taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact
     3781 tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga
     3841 agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg
     3901 gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc
     3961 tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact
     4021 tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta
     4081 tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag
     4141 tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt
     4201 ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat
     4261 atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg
     4321 cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa
     4381 acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta
     4441 gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca
     4501 aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa
     4561 gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca
     4621 aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa
     4681 tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg
     4741 aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag
     4801 ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt
     4861 atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta
     4921 aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca
     4981 ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa
     5041 aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac
     5101 aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag
     5161 atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa
     5221 aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg
     5281 gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt
     5341 ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt
     5401 gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta
     5461 aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa
     5521 gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg
     5581 atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt
     5641 gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca
     5701 cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca
     5761 ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa
     5821 aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat
     5881 cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca
     5941 ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg
     6001 gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact
     6061 ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga
     6121 ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa
     6181 tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt
     6241 cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat
     6301 atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga
     6361 aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct
     6421 agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg atgtttggag
     6481 gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa
     6541 caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca
     6601 taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca
     6661 aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga
     6721 actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat
     6781 cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga
     6841 gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa
     6901 cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa
     6961 tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga
     7021 attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta
     7081 aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt
     7141 ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt
     7201 aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt
     7261 tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat
     7321 gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt
     7381 aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa
     7441 gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac
     7501 catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc
     7561 atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta
     7621 cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg
     7681 gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat
     7741 gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg
     7801 gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc
     7861 catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg
     7921 cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta
     7981 aaataaaata caatttacaa agatagctga aattcatcta atctatagat gttttcagct
     8041 atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta
     8101 tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca
     8161 agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag
     8221 cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc
     8281 aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt
     8341 tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc
     8401 tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt
     8461 tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc
     8521 cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt
     8581 atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca
     8641 aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg
     8701 acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt
     8761 gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa
     8821 cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc
     8881 aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga
     8941 acaccgttta tt
//
LOCUS       DAYYCM010000096         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000096 DAYYCM010000000
VERSION     DAYYCM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.100"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHAY1_002588"
     CDS             complement(13..1152)
                     /locus_tag="ACHAY1_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8594532.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHAY1_002589"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHAY1_002589"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8594533.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHAY1_002590"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHAY1_002590"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8594534.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHAY1_002591"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHAY1_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8594535.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHAY1_002592"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHAY1_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8594536.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHAY1_002593"
     CDS             complement(6166..7257)
                     /locus_tag="ACHAY1_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8594537.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHAY1_002594"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHAY1_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8594538.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHAY1_002595"
     tRNA            8800..8871
                     /locus_tag="ACHAY1_002595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYCM010000097         8793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000097 DAYYCM010000000
VERSION     DAYYCM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.101"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..212)
                     /locus_tag="ACHAY1_002596"
     CDS             complement(<1..212)
                     /locus_tag="ACHAY1_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594539.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVS"
     gene            374..1483
                     /gene="pdhA"
                     /locus_tag="ACHAY1_002597"
     CDS             374..1483
                     /gene="pdhA"
                     /locus_tag="ACHAY1_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8594540.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1486..2463
                     /locus_tag="ACHAY1_002598"
     CDS             1486..2463
                     /locus_tag="ACHAY1_002598"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8594541.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2488..4131
                     /locus_tag="ACHAY1_002599"
     CDS             2488..4131
                     /locus_tag="ACHAY1_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8594542.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4139..5545
                     /gene="lpdA"
                     /locus_tag="ACHAY1_002600"
     CDS             4139..5545
                     /gene="lpdA"
                     /locus_tag="ACHAY1_002600"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8594543.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5707..5982
                     /locus_tag="ACHAY1_002601"
     CDS             5707..5982
                     /locus_tag="ACHAY1_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8594544.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5988..6755
                     /locus_tag="ACHAY1_002602"
     CDS             5988..6755
                     /locus_tag="ACHAY1_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8594545.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6923..8752
                     /gene="typA"
                     /locus_tag="ACHAY1_002603"
     CDS             6923..8752
                     /gene="typA"
                     /locus_tag="ACHAY1_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8594546.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         2993 a         1585 c         1948 g         2267 t
ORIGIN      
        1 aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa ataaaataaa
       61 tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg gttgtaagtt
      121 tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat tgtgatttta
      181 ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga aaatttgtgc
      241 ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg ttaaatagtg
      301 atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata gatgagagag
      361 gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac tgctagatga
      421 agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg tagttaacaa
      481 agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc atatggtatg
      541 gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc taggcttttt
      601 tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat ttgataaaga
      661 agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg gtttaccttt
      721 atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc cggaagaatt
      781 gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc
      841 attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg
      901 ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc atgcaaatgc
      961 cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa aacaaactgc
     1021 agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc aagtcgatgg
     1081 aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg cgatcagtgg
     1141 aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata cactatctgg
     1201 tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga aaaaagatcc
     1261 attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag aaaaagaaga
     1321 acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag ccgacaaagt
     1381 accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc cacaatcaat
     1441 caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca
     1501 atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga gaatgtcgtt
     1561 gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga aggattgcag
     1621 gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg aatcgcagga
     1681 ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca attctttggt
     1741 tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg
     1801 agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg tgttcataca
     1861 ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg tattcgagta
     1921 gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat tagaagtaac
     1981 gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga agaagtgccg
     2041 gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg aacagatgta
     2101 tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga caatcttgca
     2161 aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt agatgtagaa
     2221 acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga agcacaacga
     2281 caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat cctttcatta
     2341 gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg acaagcagaa
     2401 aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat cgcggaattt
     2461 taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaattgcc ggatatcggt
     2521 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
     2581 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat cccgtcacct
     2641 gttacaggaa cggttaaaaa tgtgattgtt ccagaaggta ccgtagcaaa tgttggggat
     2701 gtattagtag aaatcgatgc accaggacac gaagacaatg aaggggacag tggtgtagcc
     2761 gcagaatccc aaactccagc taaaccggct gctgaaccca ctgttgatac agaatcagct
     2821 ggatcgtcaa gcgaaggagt cttccaattt aagttaccgg atatcggtga aggaatcgca
     2881 gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga agatgataca
     2941 ttactagaag ttcaaaatga caaatcagta gaagaaattc cgtcacctgt cacagggaca
     3001 gttaaaaacg tgattgttcc agaaggcact gtagcaaatg ttggggatgt attagtagaa
     3061 atcgatgctc ctggacacaa tagtgcaccg tcaactagtg cgccatcagc tgaagcacca
     3121 aaagaaaaag tggaaacatc aggttcagca agtgtcgttg aagctgccga tccaaataaa
     3181 cgtgtattgg ctatgccatc tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca
     3241 caagtaactg caactggaaa aggtggcaga gtaacaaaag aggacatcga aaacttcttg
     3301 gcaggtggtc caagcagtgc accagcgaaa tcagaagcgc cagaagcggc agcaccgaaa
     3361 gaagcagctc ctgcagcaga aagcaaacca gctgcaccag caaaaccatt caagtccaac
     3421 ttaggagact tggaagaacg tgtagcaatg acgccaacac gtaaagctat cgctaaagca
     3481 atggtcaaca gtaagcatac agctccacac gtgacattgc atgatgaagt agaagtgtct
     3541 aaactatggg ataatcggaa acgatttaaa gaagttgctg ctgcaaatgg tacgaaactt
     3601 actttcttgc catatgtcgt gaaagcttta acagcgactg ttaagaaata cccagtattg
     3661 aatgcatcta tcgacgatgc aaaccaagaa atcgtttata aacattacta caatatcggt
     3721 attgcaactg atacggatca cggactatat gtaccaaatg tgaaagatgc agaccgcaaa
     3781 ggaatgtttg ctattgcaga cgaaatcaat gagaaagcaa aattagctca tgacggcaaa
     3841 ctatctgcag aagatatgcg caatggaaca attacgatca gcaatatcgg atctgtaggt
     3901 ggcggttggt ttacaccagt catcaactat ccagaagtag ctattttagg tgtaggaacg
     3961 attgcacaac aaccaatcgt aaatgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa
     4021 ctgtcattaa gtttcgatca ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaat
     4081 aatatcaaac gtcttttagc tgatccagag ctattaatga tggaaggatg ataaacaaat
     4141 ggtagttgga gattttgctg tagaattaga tacagttgta attggtgcgg gacctggtgg
     4201 ctatgtagct gccatccgcg ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga
     4261 gtatattggc ggtgtttgtt tgaatgtcgg ctgtattcct tcaaaagcac tgatagctgc
     4321 aggacaccat tatcaagagt cactagattc tgaaatgttt ggagtaacta gtgaaaacgt
     4381 caaactagat tttgcaaaaa cacaagaatg gaaagaaaac aaagttgtcc atacgttgac
     4441 ttctggtgtt ggtttcctat taaaaaaaca taaggttgaa acaatcgaag gagaagcgtt
     4501 cttcgtagat gatcacacac tacgcgtcat tcatccagat tcagctcaaa cttattcgtt
     4561 caataacgca atcatcgcaa caggttcccg tccaatcgag attccaggat ttaaattcgg
     4621 aggacgtgtg ttagattcta ctggcggatt agcattgaaa gaagtaccta agaaatttgt
     4681 gatcatcggt ggcggtgtga ttggtgccga attgggcgga gcgtacgcaa acttaggagc
     4741 agaagtaacg attcttgaag gctcaccaca aattttacca acttacgaaa aagatctagt
     4801 aaaattagta gaagatgatt tcaagaaaaa aggcgtaaca gtcgtaacga atgcaatggc
     4861 gaaagaagca gtcgataacg gtgacagcgt gacagtgaaa tatgcagtgg acgggaaaga
     4921 agaatccgtt actgctgatt atgtgatggt cactgtaggt cgtcgtccga atacagatga
     4981 tatgggctta gaacaagcgg gtgtcgaagt aggagaacga ggattgatca cggtggataa
     5041 acaaggacgt acgaacgtac ctaacatcta tgccatcggt gatatcgttc caggggcggc
     5101 attagcacac aaagccagtt atgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa
     5161 agtagctgta gattataaag caatgccggc agtagccttc actgatcctg aattggcttc
     5221 tgtaggtatg acgatcaaag aagcaaaaga tgctggtatc gaagcaaaag catataaatt
     5281 cccattttca gggaacggtc gggcattatc gcttggtaaa acagaaggat tcatccgtct
     5341 agttacgacg atcgaagata acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag
     5401 tgatatggtc tctgaattag ctttagcaat cgaatcagga atgaatgctg aagatatcgc
     5461 cttgacgatt catcctcatc catctcttgg cgaaatcgtc atggatgctt cggaattagc
     5521 attaggttta ccaatccata tttgatttga ataatacagg cgaaacgcaa ctgaaaaatc
     5581 agttgcgttt tgttttttag aattatcaat aatattatta tgtaaactta aaaatatgac
     5641 aacttcttgg tttattacca cttcctatgc tacactgaag aggaatatga aataggagag
     5701 ttgaagatgc aagaatatca gtatccactt gatttagatt ggacaacaga cgaaatggtc
     5761 attgtaatga agatgtggga agatctggaa cgtgcgaatg aaaaagggat aaacaatgaa
     5821 gcttttttga agacttatca gcagtttaaa actgttgtca aatccattgg tgaggaaaaa
     5881 cgattgggac gggaatttga aaaagcttcc ggttattctt tatatcgtac agtgcagcaa
     5941 gcaaaaaaaa atccaaacaa aactttgaga atgaacgggt gagataaatg gaacagatga
     6001 ttgatcatat caagtcctgg cttgaagaag ctggtgagaa aatcaaatct tctcaacaaa
     6061 cattaacaat ctcgcaaaaa tcaaatcgaa cggatctagt aacaaatgtc gatcgaaaca
     6121 tccagaattt tctaatcgaa aaaatccatt cctttgatcc agaggcgaaa atattggcag
     6181 aagaagaagg gtgtaatcaa ttatcggatc tcgatggaag agtctttatc atcgatccta
     6241 ttgatggcac cttgaatttt gtgattgaag gggaaaattt ttgtattatg ctcgctgttt
     6301 atgaatccgg cgttggaaag cttggtttca tttatgacgt gatgagaaat gaactttttt
     6361 ggggtggaaa aggaataggc gtgtataaaa atgatgagcc attgcctaga ccagcaaata
     6421 aatccttatc ggaaggctta cttggtgtga atagttatct tttcggtcat aataaatatg
     6481 gaatccgaga aattgcggaa agcagcatgg ggacacgtat gtacggatgc gctggtcttg
     6541 aactagtggc aatcttgaaa ggaaaccaca ttgcttatat ttcaaacctt agcccttggg
     6601 attatgcagc gggaaatgtc ttattggaag agtttggtat ggtaacaagc ggtctttttg
     6661 gtactccttt gacatttgaa aaaagggaat actatttagc agggacttct tgtgcatatg
     6721 cagaaatcgc agaccttttg aaaaaagaag gctgaaaaaa aggtagaaat cattcacaaa
     6781 aatgataaaa acgagcaaaa gattaatttt ctcgaaaaaa tgaaaacatt ttactatatt
     6841 tcatgaaaaa agtctgctat aattagcagt cgagtaaaat ttgccctgtc ataaagaacc
     6901 aataaaaaag gagttagtta cattgaatta tagaaacgat atccgtaacg tcgcaattat
     6961 tgcccacgtt gaccacggaa aaacaacatt agttgatgaa ctattaaaac aatcagatac
     7021 attagacgca catacgcaat tgcaagaacg tgcgatggac tcaaatgcgc tagaaaaaga
     7081 acgtggtatc acgattttag ccaaaaacac agctgtagac tataaaggta ttcgcgtaaa
     7141 catcatggat actccaggac acgcggactt cggtggcgaa gtagaacgga tcatgaaaat
     7201 ggttgacggc gttgtgttgg tcgtagatgc gtatgaagga acaatgcctc aaacacgttt
     7261 tgtattgaaa aaagcactgg aacaacatat cacaccaatc gttgtcgtaa acaagattga
     7321 caaaccttct gctcgtcctg agcatgtcgt agatgaagtt cttgaactat tcatcgaatt
     7381 aggagcagat gatgatcagt tagactttcc tgtgatttat gcatctgcac taaatggtac
     7441 gtcaagcttg tcagatgatc cagcagatca agaaccaacc atggcaccta tttttgatac
     7501 aattattgaa aaaatccctg ctcctgtcga taacagcgac gaaccattac aattccaagt
     7561 ttcattactt gactacaacg attatgtagg tcgtatcgga atcggacgtg tatttagagg
     7621 aacaatcaaa gtaggagacc aagtagcatt aattaaatta gacggaacag ttaagaaatt
     7681 ccgtgtaacg aaattatttg gtttctttgg actaaaacgt ctagagattc aagaagcaaa
     7741 agctggagac ttgattgctg tctcaggtat ggaagatatc tttgtcggtg aaacagttac
     7801 tcctgtggat catcaagatg cgttacctat ccttcacatt gacgaaccaa cattgcaaat
     7861 gactttctta gttaataatt caccatttgc cggtcgagaa ggtaaattcg taaccgctcg
     7921 taagattgaa gaacgtttga tggcagaact tcaaacagat gtatccttac gagttgaacc
     7981 aacaaactct ccagatgcat ggacagtttc tggtcgtgga gaattgcatt tgtctatctt
     8041 gattgaaaac atgcgccgtg agggatacga attgcaagta tctcgtccag aagttattga
     8101 aaaagaaatc gacggtgtaa aatgcgaacc gtttgaacgc gtgcaaatcg atacaccaga
     8161 agaatatatg ggtagtgtta tcgagtcctt gagcttacgt aaaggtgaaa tgcaagatat
     8221 ggtccataca ggaaacggac aaatccgttt gacattctta acacctgccc gcggtttgat
     8281 tggttattca actgaattcc tttcaatgac acgtggttac ggaatcatga accatacttt
     8341 tgaccaatat ctgccaatgc ttcctggtca aattggcgga cgtcaccaag gggcgcttgt
     8401 ttcgatcgat acaggaaaag caacgactta ttctatcatg agtatcgaag aacgcggtac
     8461 agtcttcgta gaaccaggta cagaagtata tgaaggaatg attatcgggg aaaacagccg
     8521 tgataatgat ttgactgtca acatcacgaa agcaaaacaa atgacaaacg tacggtctgc
     8581 tactaaagac caaacatctg tcatcaaaaa accaaaacaa ttgacgttgg aagaatcttt
     8641 ggaattcttg aatgatgatg aatattgcga agtaacacca gaaagcattc gcttgagaaa
     8701 acaaatttta gagaaaaatg ctcgtgaaaa agcatctaaa aagaaaaaat aatctgtaaa
     8761 aggatgaaga gagaaaccag cttgttataa aaa
//
LOCUS       DAYYCM010000098         8674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000098 DAYYCM010000000
VERSION     DAYYCM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.102"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(404..2039)
                     /locus_tag="ACHAY1_002604"
                     /pseudo
     CDS             complement(404..2039)
                     /locus_tag="ACHAY1_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2478..3347)
                     /gene="scrK"
                     /locus_tag="ACHAY1_002605"
     CDS             complement(2478..3347)
                     /gene="scrK"
                     /locus_tag="ACHAY1_002605"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8594547.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3367..4488)
                     /locus_tag="ACHAY1_002606"
     CDS             complement(3367..4488)
                     /locus_tag="ACHAY1_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8594548.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4490..4885)
                     /locus_tag="ACHAY1_002607"
     CDS             complement(4490..4885)
                     /locus_tag="ACHAY1_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8594549.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4904..5701)
                     /locus_tag="ACHAY1_002608"
     CDS             complement(4904..5701)
                     /locus_tag="ACHAY1_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8594550.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5703..6491)
                     /locus_tag="ACHAY1_002609"
     CDS             complement(5703..6491)
                     /locus_tag="ACHAY1_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8594551.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6505..6981)
                     /locus_tag="ACHAY1_002610"
     CDS             complement(6505..6981)
                     /locus_tag="ACHAY1_002610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8594552.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7053..>8674)
                     /locus_tag="ACHAY1_002611"
     CDS             complement(7053..>8674)
                     /locus_tag="ACHAY1_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8594553.1"
                     /translation="QMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELI
                     EYLYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDF
                     QIEFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDI
                     YLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAID
                     MPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVS
                     TRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKL
                     KRILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSI
                     EDMILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVS
                     DAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTH
                     FKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         2803 a         1734 c         1252 g         2885 t
ORIGIN      
        1 ttcacctggc atatattgca gattagaagc atgtaatttt ttctcagaaa caaaccactc
       61 attggaaaag acatgaatca aatcatcact tacttgtgta aaatagcgac gtttaatgac
      121 aaagatatct tcattgctat ctagtgtacc ttcatttctt tgtgcttaaa ttcaaagata
      181 attttctcaa taaagatttt tgattccagt aagattcctt tactcctttt acaacatagc
      241 gttgtccttt gttttgaaac atcttcttcc cctctttgcc attaaaaata gcccaattct
      301 ctataaagaa gattaagctt cattattata atataaagtt taaatttggt ttattactta
      361 ctttttctta ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta
      421 tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa
      481 ttaaaaaagc taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc
      541 cttgtgttcc ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact
      601 tttgcgactg ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca
      661 tgtactgtat agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg
      721 ccaattctta cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca
      781 tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt
      841 accaagtgat ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat
      901 acagccaaca gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat
      961 gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag
     1021 attcgccaag gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa
     1081 aatttggtgg ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga
     1141 ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc
     1201 gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct
     1261 tggtgttgta aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac
     1321 atcggatacc aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc
     1381 acaatcaacc ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact
     1441 agtaagccaa cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag
     1501 ctcatactga ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca
     1561 taatacataa ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga
     1621 taaatatcaa tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca
     1681 tcgattcttt ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc
     1741 aatagccaaa catataagat ataactaacg attaaattat tacttatcca ataaaaaatg
     1801 actgccggaa aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta
     1861 aacgtcgtaa aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa
     1921 tggaaggcaa tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa
     1981 taaagtccag caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt
     2041 acttaattat atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa
     2101 atcgttattt atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa
     2161 tggtctgaac accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt
     2221 aattatgtgg ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat
     2281 ctttcataaa aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta
     2341 ctatctttca ttcttcttgt ctcacttatg ataaggcgct gggtatcatg agtgaatgat
     2401 attgctgaat acaaatagtc cgaccacatt aagttgatcg gacttcataa gcattttatt
     2461 gactaatact tttttgctta agaattgatt ctaacatctt ttgctaaagt taagcatcct
     2521 atagttcctg cattatctgc taacttaggt gtgacaatgt attcttcgat atttggcgta
     2581 tcgacatagc cgttaattag ttctaaaaaa cgtctttgta ctttcttttt cagatgttct
     2641 tgttgcatga caccaccacc aaaaattata atatctggtg aaagcatcag ggtcgtatta
     2701 caggcacatt gtgcaatata atccgcctcg atttcccaga acggatcttc tatagaaaga
     2761 tcttgtccct tggttcccgt acgcatctcg atagcgggtc cagatgccaa cccttcgaga
     2821 caatcatgat gaaaaggaca agtaccagag aaagtatctc ctgaatgtct cttaactaac
     2881 gtatgcccca tttctggatg gctaaaccct tcaacaaaaa tcccatcttg tatagctcct
     2941 ccaccaattc cagttccaac agtgtaataa acaacactgg ataaaccttt tccatgacct
     3001 gaaacatact ccccgtaaca agcagcattc acatcggttg tccaggcaat tggaatttcg
     3061 aaatgcttat tcaacgttcc aacaaaatca aaatttttcc aggcagtttt tggagtagag
     3121 gtgatatgtc cataggtctt tgattcacga tggatatcaa tcggtccaaa tgaccctact
     3181 ccaatgcttt ctaattgttc tttatagtga ttaaagaaat caattaccaa actcattgtt
     3241 tcattcggtg tagttgtagg aaaactcact ctttctaaaa cctttaaatc atcattgcca
     3301 atggcacaaa caaatttagt cccacctgct tctattgagc catacatttc ttactccacc
     3361 gtccttttat ctaggaattc gttcttccct gtctaatagt ttttcgaaaa actcatgtgg
     3421 ttccttttgg taccaatagg caacagaaga aatatcgtct tgacgctcat aaagcttgat
     3481 gtcatcattt cctagggttt ggaaagtaac acgtaaattt tctttgaaac taatggggtc
     3541 gggtaaatgc cagcggtaca acccatgcat cggtaaagca tcatcgccaa aaccatgaac
     3601 tggatttgga ttaggttttc cagcactaaa ataatcccgg gtgtgatctc ttttagattg
     3661 aaaaggatag cccataaata gcgtattaaa attttgagca tatgcccttt ctccaaagtt
     3721 ctttttatgg aaagcccaag cgccgccaaa ataatcttct gctcctgtag atgttattgt
     3781 agggaaatct tcatctccat ctatataaaa cttaaattct ccttcccccc accagtatct
     3841 ttccaatgcg cacaaagcaa agtaggttcc tacataatac ccagtgcctt tgattccatc
     3901 aataagggtg taatcttttc cttttgtcgt gatgggttca cggttccatt tagcatgaaa
     3961 atacatcgca tcatccggta cttcagtcaa tgcataatta aatgtataaa aaatgtgctt
     4021 aataaactca ggatgttcat tcgttaaagt aatcttagct ttttttcgaa atggcatctc
     4081 aaaatagcaa ttcatccctg atgttggatt cactacgatt ggtaaggaat tcacttcgca
     4141 tcttgtccca aacccattac aaaaaaaatc tcctaatgga ctttctacag aaggagtgga
     4201 ttcatcatcc caataaatac gcaaaattaa atcacgtaag acgaaactcc cagcgattgt
     4261 tttgtctgtc actgtcatcc aaaaatgacg taaaattcca gggccttcca tttctgctaa
     4321 agtaattgtc tctccagcat ttatcggaac agaaggtgac ccttttctcc caattcctag
     4381 atgactcgct gtcattgccg ctttcccttt ttcaccattt ttattttctg gtgaaatagt
     4441 tctactttcc tctcccttgt ggagccatat attatttaaa caattcattt tattcgtcct
     4501 ctatcctttc ttcacttttt tgattattca tatagatgat ttgttgttgt gcctccgcaa
     4561 taattgctcc aatatctaaa ttatcatttc ctaactgcaa agcgattgac attgccaagg
     4621 gaagattcat tcctgcaatt acgtgcactc tgtcagaaat ataggtaaaa aacttttgat
     4681 ttacacttcc tccagccatg tcagttaaaa caattatttc gtctgtagta tccactaact
     4741 ctttaatttg ttcatctata tcatcaattt cttcgacata tgcacataag ctactaattt
     4801 gattatgcat acctgttaaa aactctagtg tctctttaaa accctcagct agtattgaat
     4861 ggctagcaat gatcaatctt ctcataattc aaatcctttt cctttacgct aatattccaa
     4921 atgcagccat aaccatcgca aaaacaataa cgagtagaat caaaatggtc atactgattt
     4981 ttttcttact gagtagataa tatgctgcag cagtaataat tactggaatt aatgttggta
     5041 aaattttgtc caacattggt tggattttca tactaacttc accaaaagtg aatttaagtg
     5101 gcgttgaaac actgataact gaagcaatta atgatccaac tactgttagc cccaatactg
     5161 aagcagcttc agttaaataa ttaatctttt caccgtattc agtaacaatc ttcattccct
     5221 gcttataacc aaattcaaag cctctcattc ggaaaaagaa gattaccatg attaaaatga
     5281 accataaaaa gattcctaca ggattatttt ctaaagccat ataagccgca attgatccca
     5341 tgatggttgg gatcatcgtc atcaaaagag agtccccaat accagcaatt ggacccatcg
     5401 taccaacttt aaagtcatta atcgcatctt ttgcttctaa tccttgcttt tcctccattg
     5461 caacacaagc acctaaaata atggctgcca gccaaggctg tgtattgtaa tactgaaagt
     5521 gattatttaa agcttcgatg tattcatcat catctttgta taattttctt aaaataggtg
     5581 acaatgcgta agtcacagat gcaccttgct gtgtttgata attaaaggta caaactgaca
     5641 tcaaccaacg atagttcgct ttcctaagat cctttttcgt tacttgatat ttttcgctca
     5701 ttttattcat cctccccaat aaacccaaca tcagaagctc ccgcagaagc agtgatttgt
     5761 gcatcttttt cagctctatg atacacatta aatgctaaag cgaatcctat gatcgccgct
     5821 ccaataatag gtaccttcaa aaaagcggct aagaaaaatc caacaagtaa ataactaatg
     5881 tatcgacctg taggcataaa gcgtaacaac atcgcaatac caacagcagg caacattttt
     5941 ccggctaatg ttagcccatc cataaaccaa ttaggtaaaa catccaaaac gtaattaaca
     6001 gcagactgtc ctaaagtaac ggcaataaat actggaattg ctgcagataa accaaagaaa
     6061 attggcccca accacaaaac actattcatt ttcttaaatt ctcgtttatt cgcatattcc
     6121 tgtgatttgc gaacaacaaa accatttaag attttgacaa ttacatctag ttgcacccct
     6181 aacataccta caggtagtcc tattgctaat ccggcttcta ctccaagctg gctaccaaat
     6241 gccgttgcaa taattgcgca cgttccatag tccggtacag atgagccacc aaaaccggca
     6301 actcctaatg acatcaattg aactgtaccg ccaactatca aacctgtttt tatatcaccc
     6361 atcaaaattc ctgtgaataa tccccaaaaa actgcgtaat ttacaaagat catcggaatc
     6421 ccataataat caacatgttt aataaatgct aatatagtca gcataataat ttgaaaaata
     6481 gtaaaatgca ttatccttac tcctttatcc taattttgat tgaatttcct gtgccttatc
     6541 cgccggaaca aattgtgtaa caatttctac accagaatcc aacaattttt tatacgtgct
     6601 gatattttca tctgatgcat aggctctaga agatagctgt aatgcattat ctttaacaac
     6661 agtaccgcca acaattagtc ggtcaattct aataccgtta tctactaaat gaaggattgt
     6721 ttccggctct ttcacgatga caaatacttt ttcctgatcg tatttcccct ttttaaaatt
     6781 cgcaatcgct gtttcttcag tgataattga tacagccatt ccagcaggtc tagctaaacg
     6841 catacttgat ttttggatgt cgttatttgc gacagaatca tcgatcacca tcactctgtt
     6901 tgctttagac attggtgccc attgcgtgac aataatccca tgcaataaac ggttatcaac
     6961 tctcgacata acaatactca taaatacttc ctcctttatc tttcattttc ttacaagcaa
     7021 ttaccatgcc aaaacaaaaa gaacttgata aatcaagctc tttttacata atcataaacg
     7081 tatttaacct catgaatcgg gatgtcgaca ctataataat gttcaacgac actgaaagat
     7141 tgcttaaaat gtgtctgaaa tttttgaaat tcctcactcg tttcattgaa ataaatttca
     7201 tcttccaaag tattttgttt tactaatcgt tcaattaagc aactaatgtg aacatataat
     7261 ccgaaacacg tattattatc aaatgaaatt cctagatcat tttgtaagat gtatatagca
     7321 tctgaaaccc gttctaataa tttagtcgga tttaatatgg tcaattgctc cattaaattc
     7381 gttagactaa aattcttcag aatattattg ttcaaaactt tcagttcatt atcattaaag
     7441 tgtctattca attgattgga gaattttctt atattcattc ctaaaatcat atcttcaatt
     7501 gaaaagaatt ctacatcttc tagatgtgga tttagtgttc caacaataaa aagaatattg
     7561 ttctctttac gaagctccga taaatagttt tcattcacta aagagtaata gtcacacgtt
     7621 tctatgacaa gatcagtatc ccttggaaag ctatcttcaa gtatcctttt taatttaaac
     7681 gaagttccta atccgcttgc acaagaacag acaatcatat ccttcttaat atttgatgat
     7741 tcataaatat aatccacatt ttgattttct tttacttgtt caagaatctg tttaagcggt
     7801 aaatctaata atatctgatt tgcaacatca atagccattc ttgttgaaac attgtttagt
     7861 agtccgaatt ttttttgagc gtctagttcc aaatttagat aaattttctc cagtgaaccc
     7921 atatcaacaa gtaaaataat ttcttcatat ccggtacact cttgtaagta tctattaatt
     7981 ttgtctgcaa tctcactact tgaagtcatt agtggcatat caatggcgtc gtatatatat
     8041 ctctcaacga tcctattagc agtacttgct atacaggttg ccgttagatc tccatgagcc
     8101 aaaatcagtg ctaaacgttt ttgctgaaat tctttactac acattaactt aaataaaata
     8161 accaaaacat ttttttgaat ttttgttaaa taaatatccc ctatttgaat aaaacgattg
     8221 actaattgaa cttctcgact acaccaacta ttaatatcat tgatgaagga atataattta
     8281 tcgtccgatg ttaattggat ttgataaatc caagaattca aaaagataga cgttatttga
     8341 agataataat agtcaaattc tatttgaaaa tctctctcta tttccttaac atactgtcta
     8401 aatcgaatct cttgataggt gggatatgtt aaagctgaat tattttggat atcaacattc
     8461 ttttgtctaa aatatctatc atatttctca aaaagctgct gcttatcagt gtctttcttc
     8521 cacgtttgat aaagatactc tataagttcg ttgatattaa aaacttcgtc tactttttcc
     8581 aaatcatcca tttgaatata ttttaattca ttctgtcttt tcataacctt catgccgttg
     8641 aaattcaccc attgtggtaa atgatacatc tgaa
//
LOCUS       DAYYCM010000099         8603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000099 DAYYCM010000000
VERSION     DAYYCM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.103"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHAY1_002612"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHAY1_002612"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8594554.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACHAY1_002613"
     CDS             complement(1098..2201)
                     /locus_tag="ACHAY1_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8594555.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHAY1_002614"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHAY1_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8594556.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACHAY1_002615"
     CDS             3528..5462
                     /locus_tag="ACHAY1_002615"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8594557.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACHAY1_002616"
     CDS             5919..6335
                     /locus_tag="ACHAY1_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8594558.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACHAY1_002617"
     CDS             6349..6915
                     /locus_tag="ACHAY1_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8594559.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACHAY1_002618"
     CDS             6938..7924
                     /locus_tag="ACHAY1_002618"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8594560.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACHAY1_002619"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACHAY1_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8594561.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1752 g         2667 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAYYCM010000100         8232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000100 DAYYCM010000000
VERSION     DAYYCM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.104"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHAY1_002620"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHAY1_002620"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8594562.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHAY1_002621"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHAY1_002621"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8594563.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHAY1_002622"
     CDS             2594..3379
                     /locus_tag="ACHAY1_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8594564.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHAY1_002623"
     CDS             3392..4243
                     /locus_tag="ACHAY1_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8594565.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHAY1_002624"
     CDS             complement(4367..4819)
                     /locus_tag="ACHAY1_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594566.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ACHAY1_002625"
     CDS             complement(5284..5652)
                     /locus_tag="ACHAY1_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8594567.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="ACHAY1_002626"
     CDS             5802..6284
                     /locus_tag="ACHAY1_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8594568.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="ACHAY1_002627"
     CDS             6321..7331
                     /locus_tag="ACHAY1_002627"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8594569.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="ACHAY1_002628"
     CDS             complement(7446..8159)
                     /locus_tag="ACHAY1_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594570.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DAYYCM010000101         7812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000101 DAYYCM010000000
VERSION     DAYYCM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.105"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHAY1_002629"
     CDS             complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHAY1_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8594571.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1384..1902)
                     /locus_tag="ACHAY1_002630"
     CDS             complement(1384..1902)
                     /locus_tag="ACHAY1_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594572.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1918..2244)
                     /locus_tag="ACHAY1_002631"
     CDS             complement(1918..2244)
                     /locus_tag="ACHAY1_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8594573.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2257..4236)
                     /locus_tag="ACHAY1_002632"
     CDS             complement(2257..4236)
                     /locus_tag="ACHAY1_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8594574.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4257..4571)
                     /locus_tag="ACHAY1_002633"
     CDS             complement(4257..4571)
                     /locus_tag="ACHAY1_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8594575.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4739..5032
                     /locus_tag="ACHAY1_002634"
     CDS             4739..5032
                     /locus_tag="ACHAY1_002634"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8594576.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5110..6222)
                     /locus_tag="ACHAY1_002635"
     CDS             complement(5110..6222)
                     /locus_tag="ACHAY1_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8594577.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6375..7223
                     /locus_tag="ACHAY1_002636"
                     /pseudo
     CDS             6375..7223
                     /locus_tag="ACHAY1_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7576..>7812)
                     /locus_tag="ACHAY1_002637"
     CDS             complement(7576..>7812)
                     /locus_tag="ACHAY1_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8594578.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2501 t
ORIGIN      
        1 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
       61 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
      121 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
      181 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
      241 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
      301 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
      361 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
      421 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
      481 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
      541 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
      601 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
      661 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
      721 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
      781 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
      841 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
      901 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
      961 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     1021 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     1081 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     1141 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     1201 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     1261 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     1321 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     1381 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     1441 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     1501 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     1561 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     1621 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     1681 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     1741 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     1801 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     1861 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     1921 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     1981 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     2041 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     2101 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     2161 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     2221 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     2281 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     2341 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     2401 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     2461 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     2521 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     2581 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     2641 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     2701 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     2761 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     2821 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     2881 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     2941 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     3001 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     3061 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     3121 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     3181 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     3241 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     3301 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     3361 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     3421 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     3481 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     3541 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     3601 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     3661 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     3721 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     3781 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     3841 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     3901 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     3961 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     4021 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     4081 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     4141 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     4201 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     4261 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     4321 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     4381 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     4441 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
     4501 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
     4561 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
     4621 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
     4681 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
     4741 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
     4801 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
     4861 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
     4921 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
     4981 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
     5041 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
     5101 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
     5161 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
     5221 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
     5281 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
     5341 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
     5401 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
     5461 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
     5521 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
     5581 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
     5641 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
     5701 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
     5761 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
     5821 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
     5881 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
     5941 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
     6001 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
     6061 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
     6121 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
     6181 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
     6241 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
     6301 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
     6361 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
     6421 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
     6481 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
     6541 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
     6601 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
     6661 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
     6721 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
     6781 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
     6841 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
     6901 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
     6961 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
     7021 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
     7081 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
     7141 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
     7201 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
     7261 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
     7321 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
     7381 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
     7441 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
     7501 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
     7561 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
     7621 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
     7681 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
     7741 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
     7801 tggtgtcgca tc
//
LOCUS       DAYYCM010000102         7733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000102 DAYYCM010000000
VERSION     DAYYCM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.106"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..958)
                     /locus_tag="ACHAY1_002638"
     CDS             complement(233..958)
                     /locus_tag="ACHAY1_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594579.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1112..1438)
                     /locus_tag="ACHAY1_002639"
     CDS             complement(1112..1438)
                     /locus_tag="ACHAY1_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594580.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1475..1840)
                     /locus_tag="ACHAY1_002640"
     CDS             complement(1475..1840)
                     /locus_tag="ACHAY1_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8594581.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1877..2092)
                     /locus_tag="ACHAY1_002641"
     CDS             complement(1877..2092)
                     /locus_tag="ACHAY1_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594582.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2221..2556
                     /locus_tag="ACHAY1_002642"
     CDS             2221..2556
                     /locus_tag="ACHAY1_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594583.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2632..4629)
                     /locus_tag="ACHAY1_002643"
     CDS             complement(2632..4629)
                     /locus_tag="ACHAY1_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594584.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4727..5002)
                     /locus_tag="ACHAY1_002644"
     CDS             complement(4727..5002)
                     /locus_tag="ACHAY1_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594585.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5234..5968
                     /locus_tag="ACHAY1_002645"
     CDS             5234..5968
                     /locus_tag="ACHAY1_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594586.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6007..7149
                     /locus_tag="ACHAY1_002646"
     CDS             6007..7149
                     /locus_tag="ACHAY1_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594587.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2617 a         1398 c         1110 g         2608 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag
      481 gtggtatagc tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt
      541 ttatatttcc atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc
      601 cttgttctaa taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt
      661 caagaagagt atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac
      721 ttgatacctc gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat
      781 ccatagaagt ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca
      841 tgtctgatac atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa
      901 ttcctccaat tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt
      961 ttcctcctat acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc
     1021 agactattat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc
     1081 ctgtttgaac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg
     1141 gtatctctac atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac
     1201 ttccattgtc taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg
     1261 tcacatcatc acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg
     1321 gtacagatga cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa
     1381 tagccaatac aacgatacct ataatgacaa acttcttatt catatttact ttattcaact
     1441 ttttatctcc tatctccaaa atgattttct ctcatcagta actcattgac atccccaata
     1501 gtacaccaaa gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat
     1561 ctttctctcg atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa
     1621 atgctaatga tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt
     1681 gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg
     1741 tgtctgttcc ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac
     1801 tttcatttgt tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca
     1861 ttcaattgct tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta
     1921 ttggtacact taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc
     1981 ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt
     2041 gggaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc
     2101 cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac
     2161 ttcaaatgtc attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta
     2221 atgaaacaga agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca
     2281 caaaaagaat tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat
     2341 tctgtaacag aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct
     2401 gtaagccatt ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat
     2461 cgctctctta gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg
     2521 caagaaaatg aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt
     2581 aacggtgggt gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa
     2641 tataattggg tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa
     2701 ttgataaata ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat
     2761 ccctgcccct ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt
     2821 cttaatatcc ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt
     2881 ttctattaaa gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt
     2941 gttaataagt tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac
     3001 ttcatgtgct tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg
     3061 aggaaatact cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa
     3121 atattgatat gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg
     3181 aatcacagaa gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa
     3241 gtttcctaac gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac
     3301 atcttcatca tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt
     3361 acatttatct atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc
     3421 ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag
     3481 caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt
     3541 gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa
     3601 atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc
     3661 tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac aattactgga
     3721 atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg
     3781 atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat
     3841 ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc
     3901 tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt
     3961 ttcattgact agttcatact ctctccctga atcactttga caatttttag aacatgtcat
     4021 ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac
     4081 tgcatctgac ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca
     4141 cccggggatg ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat
     4201 tgcttctttt aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact
     4261 tttttgtatc gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct
     4321 taagtatcta ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt
     4381 gttatcgaca tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt
     4441 attctttgtc ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc
     4501 tcttgtaact tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt
     4561 tgtaactggc ttctctaata atttttcttt atctacataa attggaatat taatcattga
     4621 tgtttccatt tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat
     4681 aaaaacaaac tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc
     4741 gttccatgaa actatcaata tcagattttt taaatctaat gaccgtatca atttttataa
     4801 caggtaaccc gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt
     4861 cacttgcctg ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca
     4921 taggctttag cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc
     4981 tatcaatgat ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc
     5041 taagaattat cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat
     5101 caaacaaaaa atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt
     5161 gtcaatagtt ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg
     5221 aggtgtggtc aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact
     5281 cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct
     5341 tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac
     5401 ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga
     5461 ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt atagagatag
     5521 cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca
     5581 gttcccacca gtacactcta atttagatac atggctggat agatattcca aattagaacc
     5641 agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag
     5701 tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga
     5761 caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga
     5821 atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac
     5881 tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa
     5941 aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat
     6001 gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat
     6061 aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt
     6121 tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac
     6181 ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact
     6241 catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat
     6301 caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa
     6361 aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact
     6421 gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa
     6481 acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag
     6541 cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc
     6601 agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac
     6661 atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt
     6721 aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact
     6781 gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt
     6841 ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac
     6901 attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt
     6961 attacacctc atgggtttag acatacacat gctagtttat tattagaagc aggagaatct
     7021 gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat
     7081 gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct
     7141 catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag
     7201 ctagaaccct tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt
     7261 ccaatgactc ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa
     7321 caaattactt tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat
     7381 atcgtatcca aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac
     7441 catgaagtta agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag
     7501 ttaatcactt tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg
     7561 tggcgtgctt ttgctagaaa atatataact gttctttata aagaaatata aggattaaac
     7621 attttttatc atagagtttt acttatctat agaattatgt ctgttcctaa ttcagtttga
     7681 tgaattaact gataaaaaaa tatctactat ttaaagatac agggataagt aag
//
LOCUS       DAYYCM010000103         7629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000103 DAYYCM010000000
VERSION     DAYYCM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.107"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..346)
                     /locus_tag="ACHAY1_002647"
                     /pseudo
     CDS             complement(143..346)
                     /locus_tag="ACHAY1_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            312..509
                     /locus_tag="ACHAY1_002648"
     CDS             312..509
                     /locus_tag="ACHAY1_002648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594588.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(552..1127)
                     /locus_tag="ACHAY1_002649"
     CDS             complement(552..1127)
                     /locus_tag="ACHAY1_002649"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8594589.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1282..1808)
                     /locus_tag="ACHAY1_002650"
                     /pseudo
     CDS             complement(1282..1808)
                     /locus_tag="ACHAY1_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2265..3776
                     /gene="gadC"
                     /locus_tag="ACHAY1_002651"
     CDS             2265..3776
                     /gene="gadC"
                     /locus_tag="ACHAY1_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8594590.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3789..5189
                     /locus_tag="ACHAY1_002652"
     CDS             3789..5189
                     /locus_tag="ACHAY1_002652"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8594591.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5354..6789)
                     /locus_tag="ACHAY1_002653"
                     /pseudo
     CDS             complement(5354..6789)
                     /locus_tag="ACHAY1_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7182..>7629
                     /locus_tag="ACHAY1_002654"
     CDS             7182..>7629
                     /locus_tag="ACHAY1_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8594592.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2397 a         1287 c         1380 g         2565 t
ORIGIN      
        1 ttttaatttt gattttcaat aaaaatatat tacaataata atcgtaatag ctaatgaaac
       61 aatttctcaa tctttttacc actccaaaca gggagcgtca ataattttgt gtaaataact
      121 cgtccttctg caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt
      181 gttggaagcc tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata
      241 ctaggaaacg ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct
      301 tgatttgttt attgaaagat tcgattgtag aaccagaagg aatggagaac acttttgaac
      361 tttacaacaa aactactgtt gataaattaa ttccaatttt agttccaact gatttaaatg
      421 gtcatgatgt tttacttcgt tttaacggat ttttaggtga ggattataga tacctaaaaa
      481 tttctgatcc tcaaccttta acagaataat aaaaaacgtg taatcaatta tttgattaca
      541 cgttttttat ttcaatcttc gttgtagtag tggttgcgac ataccgaaag gtaatcttca
      601 ttacctccta ctacaatctg ttcacctgaa taaactggaa taccatcaac aatacgagca
      661 ttcattacag cctttttatt gcaaaaatgg caaatagttt taatttcctc aattttatca
      721 gcaaatgtca ataatgcttc ggatcccttg aagagattat tttgaaaatc attcttcaat
      781 ccatagcaaa ttactggtac ttcctcattc attacgataa gtgatagatc aataatattc
      841 tctctactta aaaattgaga ctcatcgacc aaaatacaat caacttttcc gtactgatca
      901 ataatctcca ctaattctcc gtacacatcc atttcagaag taattgcaat agctggtttc
      961 gaaacaccta tacgactctc aacttttcca aatcctgatc gagtatctat tgcgcttgtc
     1021 attatgataa ctctcttccc ttgttcttca taattatgag ccactttcag tagctcaata
     1081 ctctttccac tattcattgt tccatatctg taaaataatt gtgccattaa atttattccc
     1141 ttcttttaca attcaattat tccttaattc catctgcttt tatgcagctg ctcgtttatt
     1201 ccttgatatg tctataacat ctactatccc taaaaccaat ttataagcca ttagagatgg
     1261 tattcctgaa catctctcat attgtttttt gagtggtata tcatctgaaa aactgaagga
     1321 atcatgaaaa tagcgacata cttatcattt agagttctaa ctttaaatgc atttacttct
     1381 tcagtcagca ctttagtcat gttggacatg gtttggggcg tgtagtgatg cccatacatt
     1441 ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag gtgaatgacg
     1501 gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg cttgaactcg
     1561 ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt gaccgtacgg
     1621 tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa
     1681 tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca
     1741 cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa
     1801 tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag
     1861 ggacttcctt ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat
     1921 taatacttgc tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt
     1981 cgcagctttt tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag
     2041 ctttttcact aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca
     2101 cttttctaaa aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact
     2161 ttgtcttgta gaaaagaggt agacaaaata atgcaagctc aaataagcga ttttccataa
     2221 ccttggtagg aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt
     2281 tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc
     2341 cgacctttgc aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat
     2401 ggtttctacc agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg
     2461 gtgggatttt cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt
     2521 ttttccaatg gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag
     2581 cgctgtcgta tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg
     2641 gtgtattagt cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg
     2701 ctaaaattgc taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg
     2761 gtttagcagc agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag
     2821 cttttattcc tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg
     2881 cttatatggg cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa
     2941 actacccatt ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg
     3001 gtttctcagt agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc
     3061 aaacatttga aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga
     3121 ttgctctcat gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat
     3181 cacgagggat gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta
     3241 ataaacaaga cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg
     3301 cagcagtctt aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct
     3361 taactgttgt catttatctc gtaggatatt tactattttt cctaggttac tttgtattag
     3421 tttttaagaa aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa
     3481 cgattatcgg tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac
     3541 caccagcttc aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct
     3601 ttgtagtgac tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca
     3661 caactattga ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag
     3721 ctgctagagg agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg
     3781 aattcattat gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt
     3841 ttggctctgc aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg
     3901 aaccacgaat tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat
     3961 taaacttagc tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc
     4021 aaacgttaga aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa
     4081 accgctgtgt aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg
     4141 gaacttcaac gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg
     4201 cttggcgtaa acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg
     4261 tgatctcttc tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac
     4321 tgagagaagt cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt
     4381 atgtggatga gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt
     4441 atgatgatat caagggtctg aatgatttag ttgaagctca caataaacaa actgactata
     4501 aagtatacat tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg
     4561 atctagtttg ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat
     4621 atggtttggt atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag
     4681 aagaattagt atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact
     4741 tctctcatag tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg
     4801 atggttatcg tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga
     4861 tcgaaaaaac tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct
     4921 ataagctgaa agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt
     4981 taatgaaggg atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta
     5041 ttcaacgctt agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag
     5101 atatgaacca agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg
     5161 atgtgaaaac gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat
     5221 taactaataa aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata
     5281 tgatattcat ttgttacact ctcaatctta gctgttgttc tattcatcat tactcttatt
     5341 gtaagtagtt cagtcaaatt tgcttgttca attgatcgat aacagtatga attgtttgaa
     5401 aagtaatcgg atatataatt gttttctcac tacctaagcc ttcttggtat aaagaactat
     5461 cgcaaagtat aatccgctga gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt
     5521 tacaaatgat attactaata gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat
     5581 agtataaata cctaaagtct gacggagcta ctatgaacac ttcaattgga gatttccgtt
     5641 tatgctcatt taaaagactt aaactaatgg ttagtttacg aatagaattc tcattccaac
     5701 gcagaaataa attgttttgt tttcgccatt gttcaagaat ttcatgaact tctaagtaca
     5761 aagcgcgttg ctcttcattt aacaagtaag tcttatcagg ttgaaatatc tgaatatcag
     5821 cccaaagtga gcggataaac gggagaagtg ctttatcaaa aagtatctga ttttcttcag
     5881 atatattcag agataagata agtttttgac tgagctcaat caccaaaggg ttatctgcta
     5941 catggttttg ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat
     6001 aatttctaga gttgaataga aagtagatat aaaggcattc atcttcgtca tgacataaag
     6061 agaaagaaag ttgctctaat cctttctgta ttagctgaaa caagggagta tgtcgggcaa
     6121 ctcttttttc ttcctgacta aaatatattg ggttagtctt agatctctgc aaaccgatag
     6181 cgatacctaa taaaataaat tgtttttcaa ctgtagggta acttcttgaa aaaaatgttt
     6241 ttctacataa tcaatcaatt gttgaccgct ttgaataatt gatttattta gctggatcaa
     6301 ctctgtccga tttgtctcat agattatcgt tagtaacaaa cttcgataat ccttttcagg
     6361 aatttcaaaa tggccatcag catttaagta ctgcatttgt ttaaaaaaat taatcaactc
     6421 atttcgtaag ttgtaaactt tgcttaacga aatgaaggta gtatcagcta tattactcca
     6481 attgtgttcc cctgataaat aatgtcctag tacattgaaa aagtcagagc gctgatagat
     6541 gagttgagtg agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa agctacttct
     6601 agatttctcc actgaaataa tttgctgatg gagtatgtcg gtaatgcatt ccaagtcgtt
     6661 agtgacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct gaatggtccc
     6721 tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct ccagataggt
     6781 ttccaacata gtactcctcc tagtcatttt atacataact attataactc taccaatcgg
     6841 aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag atggggtatt
     6901 tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga attaataccc
     6961 gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt tttgctttta
     7021 tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca tgttgataaa
     7081 gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa tcggattctt
     7141 gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa gacataactt
     7201 tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac gattataatc
     7261 ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt gaagatggct
     7321 ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc ttggttgatg
     7381 tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta ccggtaacag
     7441 ttattgataa acccatagaa gaacgtatgg cttcaacaat tcgacacaat cgtgcaagag
     7501 ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa aatggatggt
     7561 cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt cgctttaagc
     7621 aagtgtctg
//
LOCUS       DAYYCM010000104         7421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000104 DAYYCM010000000
VERSION     DAYYCM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.108"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..249)
                     /locus_tag="ACHAY1_002655"
     CDS             complement(<1..249)
                     /locus_tag="ACHAY1_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594593.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISL"
     gene            complement(367..627)
                     /locus_tag="ACHAY1_002656"
     CDS             complement(367..627)
                     /locus_tag="ACHAY1_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594594.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(764..1177)
                     /locus_tag="ACHAY1_002657"
     CDS             complement(764..1177)
                     /locus_tag="ACHAY1_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8594595.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1633..2118)
                     /locus_tag="ACHAY1_002658"
     CDS             complement(1633..2118)
                     /locus_tag="ACHAY1_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594596.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2257..2472)
                     /locus_tag="ACHAY1_002659"
     CDS             complement(2257..2472)
                     /locus_tag="ACHAY1_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8594597.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2696..3496
                     /locus_tag="ACHAY1_002660"
     CDS             2696..3496
                     /locus_tag="ACHAY1_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8594598.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3515..5383
                     /locus_tag="ACHAY1_002661"
     CDS             3515..5383
                     /locus_tag="ACHAY1_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8594599.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5376..5867
                     /locus_tag="ACHAY1_002662"
     CDS             5376..5867
                     /locus_tag="ACHAY1_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8594600.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5855..6409
                     /locus_tag="ACHAY1_002663"
     CDS             5855..6409
                     /locus_tag="ACHAY1_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8594601.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6427..>7421
                     /locus_tag="ACHAY1_002664"
     CDS             6427..>7421
                     /locus_tag="ACHAY1_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8594602.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2633 a         1193 c         1418 g         2177 t
ORIGIN      
        1 aagagagatc ataagcccca atgaatgact aaaaagaaaa gcagaatcac atgccatatc
       61 atcccatatt tcttcaagaa aataaataag cgggaaaaag ggaaggaata gtagaacatc
      121 gatccagcac catttttttc ttcttttgaa ataacataaa acaataaaca tgaacgtgca
      181 gggaagtaaa aacaaagctt tactcataac taacaaaata ccagctcctt ttattttttt
      241 ctcccccaca ttcaaagagt aacaatggta tgtacttaat aatagaaaaa tggatcaact
      301 tgtatgaata acagctgaaa agatctgcac caaataaaaa acttccagcc aaaactgact
      361 ggaagtttat ttttttctga aatattctaa cacaaatatg aaaaagatgg ccgaagctaa
      421 taagccgatt tcgtagcaaa acaatgagat accagagtcg taataataaa atcctctaat
      481 cttatggata aaatagatca tcggacaagc agataagagt aagtatttgt taggcctttt
      541 gaataagtaa aagaaagcac tggtcattat gataaatggt atccaaaaca aaagtttact
      601 taagacaatc aaatgatcaa ctcccatatt ttttaccaag agagatcata acatctttat
      661 cgtaaaaaca aaatattttt ttgctcattt tgaaaattta aacatactcg ttcttatcca
      721 aatatataaa aatcgcccct cattcatcga gaggcgataa gttctaattt aactcaattt
      781 tttttgtttc ttttggctga taagctgttt taggtaatac caatttcagc acaccgtttt
      841 taaaggttcc agtgattttt tcttcatcaa cattaggaag atagaatgaa cgtttgtaag
      901 agaccgaatg tctttctttc cgtacatagt tgccatcttc tttatcttct gtatgggact
      961 catgattggc ggagatcgtc aaagtattat ctgaatattc aactgtcgtg tcttctttgt
     1021 cacagccagg catatcagca gttagtttgt attctttttc attttcgact aaatctactt
     1081 ttggataact tgaaacacct aaaaaatcat tgaatgctgg actaaacacg tcattgaaat
     1141 cagggaacat gtcgcgaatg ctaggtacat ttgccatttt tgatcatcct ttcaataagt
     1201 attttttatt tatccctatt tacgtaataa ctctattttg aaaaaaatgc aagtataatg
     1261 gttagctttg tgaatatttc atctaatacg atacaaaggc tatctttcta atttgaaaaa
     1321 gggatcatta aaataataag aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat
     1381 ccaaatatag aaaaattgaa tctagaaaac cgcccgacaa taaatttcat taactcagtt
     1441 gaccgaaaat gaagtagata ccaaaaattt atcaagagag caagtagaga agtgagtata
     1501 ttcataagag aaatagctgt aacgaattat taggttaatg caaatgctta tttatactcc
     1561 gtataacttt ctcagaaaaa atgataccaa taatgacttg ctagtactgt aaatccgtat
     1621 aatatcatct aatcaaaatg gtgttccatt tgcatctgtc atcgtaatcg atccttcggg
     1681 tgacactcga taaaaaccaa cgcttccaga tcctccttgg gaacggattt gtttgctgga
     1741 agctgtgaac aaataatctc gatcaatttt ttgataaaag ctaataacaa tatcgtcact
     1801 ttgtaattcg gaacggtggg caataagaaa tagaagtgct ttgtcagagt tttctaatgg
     1861 aagagaaggt acagtgcttt cttgcatttc agatgttgaa gaaatgctaa acgactgaat
     1921 acttgattgg ctatgttgcg aatctacatt accagtagaa gtattagtaa tggaagtagt
     1981 agaagtactt ttagttgaac caacctcagt tgttgaaatg gctgctgaag attctttaac
     2041 agaattcttt gttttatatc cacaggcaga aaaaagtatt acagttccca acaagcctgc
     2101 gtaaatccct ttatgcatga tagctcctcc taaaatagat tatctttatt cagtatatca
     2161 cttaaacaaa gaaaagcaga tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt
     2221 catgaagata ataacttttt taataattat agctttctaa tatctttcat caacttttct
     2281 agtaatcatt gggaaaaatt tcaagcagta gatcgcacaa atacctacga cgatataaat
     2341 gatcttcgca aaaatagttg ttgaaccacc agcaatcata gctactaaat caagttcgaa
     2401 caagcctacc aacagccagt tcaaccctcc cacaatcaat aaagttaatg caatagcatc
     2461 taaagttttc attagaattc tcctttctta aatgataaaa caattatata tcaaaaagag
     2521 aaacattgct tatataacgc tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt
     2581 ttttgctctt ccattagtac acatttttta gctgaagctg taaatttcat gtcttaattt
     2641 tttttagtga gcttttatac tggatttgta agaaaaaaga aggagaggat cacatatgtc
     2701 ggattggttg gatattgcag gaaaagtagt aattgtaact ggaggttcct caggaattgg
     2761 aagaagtatc gtcgaaaatt tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac
     2821 tgaatgccgt atacagcatg aaaacctact atctttaaaa gtcgatgtgt cttctaaaac
     2881 ggacatagaa gaagggatat ataaagtaat gaaacatttc aagacgatcg atggtctagt
     2941 caataatgca ggaataaata ttccttctct tctaatagat agaaatcatc caaaaagcaa
     3001 gtatgaatta tcagaacaag tttttgataa gatgatcgct gtcaatcaaa aaagtgtata
     3061 tttgatgagc caagcagttg gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa
     3121 tctttcttct gaatcaggat tggaaggatc agaaggacag agctgttatg ctgctacaaa
     3181 agcagcgatg aacagtttta ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg
     3241 tgtggtaggt gttgcaccag ggatactgga agaaaccggt ttgagaacac aagaatatga
     3301 agaagctctt tcttatacga gaggtataag cgtcgaacag ctgagaaacg gttacagtcg
     3361 aacaagtacg atccctctag gaaggagtgg taagctgcaa gaagtggcgg atctagtctg
     3421 ctattatctt tcagaaagat caagttatat cactggagtc acgactaata tttcaggagg
     3481 taaaacaaga ggataaagga taggggggac gcacgtgacg ctagtaaaca gatggtacca
     3541 attattgaaa ttactagtca ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat
     3601 caaaaccagt aatcaaacca taaaaaaaga tattggggag ttgaatgaag aaattgccgg
     3661 tattgcaaaa atcatccaaa aaaacaaaca ttttgaactt gaaatttatg attttgattt
     3721 ttttgatgag atcatgcaag gaaaattgaa aacagccagt gatttcaatt cggcatccaa
     3781 aagaatcgct tatttactaa ataggctgat cgaaacagag gatttcttac gtatggatga
     3841 tttgtctgaa gaagtcggtg tcagcagagg aacagtggtc aaggatctaa agacattaaa
     3901 agagatgatc aatgattttg atgtaaagat tacaggcaca ccaaataaag gactaagaat
     3961 aataggagat gagctagaat tacgactact tttattttat tttgtttctc cctacttttc
     4021 agagacgaaa acagaacagt ttctagaaga aaaacaagca atcaaacatt ttcagaagaa
     4081 gacgaatgct agtaaacaag aaatcaccct tttaacgaaa gttatcggta tttctttgaa
     4141 tagaatctct agtaactatc tgttgaagaa gcccatcaat cgttacagtg ggtgttttga
     4201 atatatccaa gaattcaatg aactgatcaa tcagttggaa gaaatctatg aactaacttt
     4261 gagccaatat gaaaaagatt ttatcagttt tcctttgaac atcatcaaca caagtataac
     4321 gatgataagg aaagctaact ctgatttgcg cttatatttc gaccccatga tgatgcgtat
     4381 tcgacgatta gatctcattg atctggatga gtccttctta tatcaagaga tgaaacagca
     4441 cctctcttta ttgataaatc gggtaatttt tcgttatcga ttgactgatt tattttatgg
     4501 tgagatagaa aaaaagtatc ctttttctta ccaaatcgct agcgaatgca tacaagcatt
     4561 agaagctatc ttgcaacata agatctctaa agtagagatc agctacctaa cgttgtattt
     4621 ggagttagca tttcgatatc aaaaagagca tttttcctct aaaaaagtag ccattgttca
     4681 tacgacagga aaaggaacag cattaatgat ccaaagacaa atcaaaagga tattggggag
     4741 ggatatacaa ataacacatg tttcagaaga acaatatgaa aaagtagatc taaacaaata
     4801 tttcgctgtt tttacaacga ttccgttaaa aaatgtaaat gaagagacac cggtgatcca
     4861 tttgactaat ctttttgatg actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa
     4921 gcgattagcc gggtttcatt ctactgtttt tttctatcaa atatggaaag aaaaaaagac
     4981 gtatcaagaa tatttaaaca aattagtcgc tcctttgatc agaggctatt atgtggatga
     5041 agaatttgaa aaaaggatct tgaaaagaga atatcagcaa agcacatttg ttaacaaaga
     5101 atttgctttt cctcacacaa ccaaccagct atctcaacac attgttttat cagtagggct
     5161 aataactgat caaggtttag cagaaacagg aggaaatgga gttatttttt tatttgccat
     5221 acctgaagaa atgacggctg aagttgagaa tgaattattt gagctatacg atatcctatt
     5281 tgaactcata aaaatgggaa tagatgagtt tattgaatta gcaccgacac aagaatcatt
     5341 tttaaaacta atcaaaagta aaggagagag acttcatgaa tgatatgtat ctattcatag
     5401 ttttcgcagc ttcagcttat gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt
     5461 tcaatcaaac atatagcgaa ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg
     5521 ggaaaatccg ttcaggcacg attgttatgc tagctataga cgatcatgat caagttttag
     5581 aagtcagaaa aatgcaaggg gttacatctt tggcaaaatt tcattcatta cctgtctatg
     5641 cgggtgaaga tatccattac atcgatcgtt accatccctc agttcgagcg gaagatcgtt
     5701 tgacacaagc agctttagaa aatgccagag aagtatactt acgaatagag gcaggaaatg
     5761 aaaaaagtga acaaccttca ttgcctttta ttgctatgct caataatttt cggatatgga
     5821 aaagtacgat caaagaaaaa aggagtgaca aacgatggat atcataacaa aatttgctga
     5881 gggatttatg aggctgttcc aaacaggtgc agaaacattc ataagctgga tgacaggaat
     5941 cgtaccagtt gtcttgatgc tgatggttgc aatgaatacg ctgattgctt ttttaggtga
     6001 agaccgagta acgaaagtag ccaaattatc agcgaaaaat ccgcttactc gttaccttgt
     6061 gttaccgttc ttatctgctt ttatgttggg aaatcctatg tcatttacaa tggctcgttt
     6121 ccttccagaa tactacaaac caagctatta tgctgctcaa gctcaatttt gtcatacgag
     6181 caatggtgta ttcccccata ttaatcctgg agaattgttt gtatggatgg ggattgctca
     6241 agggatcgaa accttagggc taaattcgat ggagctggct atccgatact tgcttgtggg
     6301 actgctgatg aactttattg gtggttggat aactgatttt actactggat ttgtatgcag
     6361 acagcaagga atcgttctta gcaaaaaagt agagctttca gttgattaga gaaaggagag
     6421 ttttaaatgt atggtgatat taagatcgta agaggaaacg gcgggtatgg aggtcctttg
     6481 attattaggc cgacaaaaga taaacacaag ttcatctatg tcacaggtgg aggtgagagg
     6541 ccaatgatcg tagataaaat agaagaactg acaggtatgg agtctatcaa tgggttcaaa
     6601 acatcaatac ctgatagtga aatcgcgctt gcaatcgttg actgtggagg gactcttaga
     6661 tgtggaattt atcctaaaaa agggatacca accatcaata tcgtttccac aggaaaaagt
     6721 ggcccattag cacaatatat cactgaagat atttatgttt cagcagtagg gttagaacaa
     6781 atacagccac taatggaaac tgatgataac aaagaaacag taaatactgc atctccagat
     6841 aaaaagtaca aatatgatac ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac
     6901 tggatagcaa aaattggaat gggcgcggga aaagtagttg ctacattcaa tcaatcagca
     6961 agagaagcag tagatacgat gatccggaca gtgatccctt ttatggcttt cgtttccctt
     7021 ttgattggtg tgatacaagg ttctgggatc ggtaatgtat ttgctaacat tatggcacct
     7081 ttagcaggaa atgcagttgg attaacaatc attggcttta tttgttcact gccattttta
     7141 tctcctttgc ttggtccagg ggcagttatt agccaagtga ttggaacatt gatcggtgta
     7201 gagattggga aaggaaatat tcctccccaa ttagcattac cggcactatt tgctatcaat
     7261 actcaaaatg catgtgattt tattccagtc ggtctaggtt tggaagaagc aaaagctgag
     7321 acagtagaag taggtgtacc atcagttctt tattcaagat ttcttaacgg catcccacga
     7381 gttatcattg cttgggtagc aagttttgga ttatatagct g
//
LOCUS       DAYYCM010000105         7141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000105 DAYYCM010000000
VERSION     DAYYCM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.109"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHAY1_002665"
     CDS             <1..615
                     /locus_tag="ACHAY1_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8594603.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHAY1_002666"
     CDS             612..1385
                     /locus_tag="ACHAY1_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8594604.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHAY1_002667"
     CDS             1382..1780
                     /locus_tag="ACHAY1_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8594605.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHAY1_002668"
     CDS             1965..2636
                     /locus_tag="ACHAY1_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594606.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHAY1_002669"
     CDS             2695..3135
                     /locus_tag="ACHAY1_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594607.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHAY1_002670"
     CDS             3147..3815
                     /locus_tag="ACHAY1_002670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8594608.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHAY1_002671"
     CDS             3879..4502
                     /locus_tag="ACHAY1_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8594609.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHAY1_002672"
     CDS             4558..4944
                     /locus_tag="ACHAY1_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8594610.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHAY1_002673"
     CDS             5157..6548
                     /locus_tag="ACHAY1_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8594611.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAYYCM010000106         6754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000106 DAYYCM010000000
VERSION     DAYYCM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.111"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHAY1_002674"
     CDS             complement(<1..182)
                     /locus_tag="ACHAY1_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8594612.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHAY1_002675"
     CDS             517..951
                     /locus_tag="ACHAY1_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8594613.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHAY1_002676"
     CDS             974..1294
                     /locus_tag="ACHAY1_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594614.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHAY1_002677"
     CDS             1435..1626
                     /locus_tag="ACHAY1_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594615.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHAY1_002678"
     CDS             complement(1697..1954)
                     /locus_tag="ACHAY1_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8594616.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHAY1_002679"
     CDS             2112..2375
                     /locus_tag="ACHAY1_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594617.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHAY1_002680"
     CDS             2399..2587
                     /locus_tag="ACHAY1_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594618.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHAY1_002681"
     CDS             complement(2670..2855)
                     /locus_tag="ACHAY1_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594619.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHAY1_002682"
     CDS             3070..3441
                     /locus_tag="ACHAY1_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8594620.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHAY1_002683"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHAY1_002683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHAY1_002684"
     CDS             complement(3709..4137)
                     /locus_tag="ACHAY1_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594621.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHAY1_002685"
     CDS             complement(4197..4955)
                     /locus_tag="ACHAY1_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8594622.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHAY1_002686"
     CDS             5250..5447
                     /locus_tag="ACHAY1_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594623.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHAY1_002687"
     CDS             5553..6035
                     /locus_tag="ACHAY1_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594624.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHAY1_002688"
     CDS             complement(6093..6689)
                     /locus_tag="ACHAY1_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8594625.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2279 a         1043 c         1334 g         2098 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agca
//
LOCUS       DAYYCM010000107         6642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000107 DAYYCM010000000
VERSION     DAYYCM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.112"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2833
                     /locus_tag="ACHAY1_002689"
     CDS             158..2833
                     /locus_tag="ACHAY1_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8594626.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3015..3254
                     /locus_tag="ACHAY1_002690"
     CDS             3015..3254
                     /locus_tag="ACHAY1_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594627.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3270..4643
                     /locus_tag="ACHAY1_002691"
     CDS             3270..4643
                     /locus_tag="ACHAY1_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594628.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4647..6281
                     /locus_tag="ACHAY1_002692"
     CDS             4647..6281
                     /locus_tag="ACHAY1_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594629.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6444..>6642
                     /locus_tag="ACHAY1_002693"
     CDS             6444..>6642
                     /locus_tag="ACHAY1_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594630.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2330 a         1071 c         1334 g         1907 t
ORIGIN      
        1 aagaaaaaag ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg
       61 gatatatttt tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca
      121 tacaaataaa aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat
      181 tcagaagcat ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt
      241 ttctacatta aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat
      301 tattaactat ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc
      361 tattaaacaa gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa
      421 aattcggttt gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag
      481 cgaaaagaaa aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag
      541 aatagaatca tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa
      601 tgaacaacca aataatcacg agacgattct tactattcag aattttcttc aattacttca
      661 ccgaagattt ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag
      721 attatgcaaa ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca
      781 tcgtttatct agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat
      841 tcttgaacag atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc
      901 atcttttaga gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta
      961 tcctgaatta acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt
     1021 tgacgaatgt ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac
     1081 ggctgataat aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca
     1141 agactttgta aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga
     1201 atccaatcaa gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt
     1261 gaaagaaggt caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa
     1321 tatcgaacaa aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga
     1381 aatagaaata gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg
     1441 cttaatcatt aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga
     1501 gaatgctcca atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat
     1561 gatttttcag ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc
     1621 atgctaccaa ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta
     1681 tgttttagga acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg
     1741 ctatttaaac gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa
     1801 gagcacaacg ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac
     1861 tacggttctt agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa
     1921 tcagggaggt gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag
     1981 ctcgttgatc tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca
     2041 gcttatgcca caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga
     2101 aaattatttc aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat
     2161 caagtttaca aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt
     2221 tggttggggt ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga
     2281 agcaactttt gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa
     2341 gcaacgcatt ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat
     2401 actgggctat cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca
     2461 gttaattgta gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt
     2521 gttggtgatg aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac
     2581 gatttgggga aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat
     2641 cccgtttgag gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag
     2701 gcaattgctt gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca
     2761 catgaatcgt caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta
     2821 ttttcgattg taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat
     2881 atttcttatt gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca
     2941 aacggaagta aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag
     3001 aaaggaaaat tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata
     3061 ttcaagaaca tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa
     3121 taagaggggt atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat
     3181 atttagaaaa tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt
     3241 tagttcattc atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg
     3301 tttatgaaga agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt
     3361 tagagaaata tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag
     3421 cggagcgtcc agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg
     3481 tgatctatgt ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta
     3541 ttgagttgtg ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt
     3601 ctttctcaca cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac
     3661 aacaacgaaa cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac
     3721 ggcaatattt gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac
     3781 aagaagtaca cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat
     3841 atggctacaa aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc
     3901 cttttcagcc ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca
     3961 aaggaggatc tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct
     4021 ttttacaagc ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt
     4081 acttattacg tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc
     4141 aatggaatac aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc
     4201 aaggaatttt tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt
     4261 tttttcaata cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg
     4321 aaaagcgaaa agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga
     4381 ttaaggcatt tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc
     4441 ttcaaaagcc gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga
     4501 aagaatggct tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc
     4561 ggattgaggt aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa
     4621 atattttaga aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt
     4681 atattcgcgt gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac
     4741 aaaatggacg acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg
     4801 aaggaatcag tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg
     4861 ctcggaacca tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata
     4921 tgttagatgt cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga
     4981 tctcggaaca atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga
     5041 gtttcggtga atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct
     5101 tagtgaaaga taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg
     5161 acacgaatgg tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga
     5221 tctatgcaaa atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc
     5281 aaggatatca aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac
     5341 gcaataaaat ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac
     5401 gacggaaggg gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt
     5461 cggaagaaga ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat
     5521 ggaaccatac aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac
     5581 cgatggcagc gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat
     5641 attattcttg ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc
     5701 gtgccgaaca agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag
     5761 aagttcttcc ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat
     5821 tggaacaaga actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa
     5881 aatgggaagc tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag
     5941 acgaattgac tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct
     6001 ttcaacaagg agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc
     6061 gttttcttgc ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga
     6121 aaataacctt tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg
     6181 ccgtggtaca acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg
     6241 ctatttttgt gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa
     6301 cgttagaggg gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga
     6361 ggtccttatc ataaataggg atatttatta atgaatattt gaaatataat tgtatataag
     6421 aattaaaaag agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat
     6481 acactgaact tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa
     6541 agaccagaac tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc
     6601 cgaaggggat taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DAYYCM010000108         6599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000108 DAYYCM010000000
VERSION     DAYYCM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.113"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHAY1_002694"
     CDS             complement(1273..1938)
                     /locus_tag="ACHAY1_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594631.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHAY1_002695"
     CDS             2064..4100
                     /locus_tag="ACHAY1_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8594632.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHAY1_002696"
     CDS             4104..4742
                     /locus_tag="ACHAY1_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594633.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHAY1_002697"
     CDS             4770..5198
                     /locus_tag="ACHAY1_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594634.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHAY1_002698"
     CDS             5655..6566
                     /locus_tag="ACHAY1_002698"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8594635.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2269 a          999 c         1219 g         2112 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaat
//
LOCUS       DAYYCM010000109         6326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000109 DAYYCM010000000
VERSION     DAYYCM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.114"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAY1_002699"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHAY1_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHAY1_002700"
     CDS             complement(1878..3080)
                     /locus_tag="ACHAY1_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594636.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHAY1_002701"
     CDS             complement(3140..3343)
                     /locus_tag="ACHAY1_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8594637.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHAY1_002702"
     CDS             complement(3485..4552)
                     /locus_tag="ACHAY1_002702"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8594638.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHAY1_002703"
     CDS             complement(4610..4717)
                     /locus_tag="ACHAY1_002703"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594639.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHAY1_002704"
     CDS             4880..5692
                     /locus_tag="ACHAY1_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594640.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHAY1_002705"
     CDS             complement(6066..6257)
                     /locus_tag="ACHAY1_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594641.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1883 a         1298 c          997 g         2148 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaa
//
LOCUS       DAYYCM010000110         6321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000110 DAYYCM010000000
VERSION     DAYYCM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.115"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACHAY1_002706"
     CDS             <1..186
                     /locus_tag="ACHAY1_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8594642.1"
                     /translation="KQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISL
                     VIYFLKIKNRIIDDYNS"
     gene            199..1206
                     /locus_tag="ACHAY1_002707"
     CDS             199..1206
                     /locus_tag="ACHAY1_002707"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8594643.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1218..2222
                     /gene="neuB"
                     /locus_tag="ACHAY1_002708"
     CDS             1218..2222
                     /gene="neuB"
                     /locus_tag="ACHAY1_002708"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8594644.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2219..3367
                     /gene="neuC"
                     /locus_tag="ACHAY1_002709"
     CDS             2219..3367
                     /gene="neuC"
                     /locus_tag="ACHAY1_002709"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8594645.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3340..4017
                     /locus_tag="ACHAY1_002710"
     CDS             3340..4017
                     /locus_tag="ACHAY1_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8594646.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3968..5149
                     /locus_tag="ACHAY1_002711"
     CDS             3968..5149
                     /locus_tag="ACHAY1_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8594647.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5162..6139
                     /locus_tag="ACHAY1_002712"
     CDS             5162..6139
                     /locus_tag="ACHAY1_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8594648.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6139..>6321
                     /locus_tag="ACHAY1_002713"
     CDS             6139..>6321
                     /locus_tag="ACHAY1_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8594649.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYD"
BASE COUNT         2361 a          790 c         1204 g         1966 t
ORIGIN      
        1 aaacaattat ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct
       61 ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg
      121 ataattagtt tagttatata ttttttaaag attaagaatc gaattataga tgactataat
      181 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
      241 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      301 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      361 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      421 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      481 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      541 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      601 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      661 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      721 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      781 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      841 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      901 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
      961 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
     1021 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
     1081 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
     1141 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     1201 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     1261 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     1321 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     1381 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
     1441 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
     1501 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
     1561 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
     1621 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
     1681 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
     1741 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
     1801 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
     1861 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
     1921 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
     1981 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
     2041 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
     2101 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
     2161 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
     2221 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
     2281 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
     2341 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
     2401 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
     2461 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     2521 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     2581 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     2641 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     2701 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     2761 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     2821 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     2881 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     2941 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     3001 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     3061 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     3121 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     3181 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     3241 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     3301 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
     3361 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
     3421 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
     3481 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
     3541 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
     3601 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
     3661 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
     3721 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
     3781 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
     3841 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
     3901 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
     3961 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
     4021 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
     4081 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
     4141 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
     4201 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
     4261 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     4321 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     4381 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     4441 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     4501 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     4561 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     4621 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     4681 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     4741 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     4801 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     4861 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     4921 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     4981 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     5041 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     5101 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     5161 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     5221 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     5281 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     5341 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     5401 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     5461 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     5521 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     5581 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     5641 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     5701 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     5761 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
     5821 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
     5881 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
     5941 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
     6001 acatactatt attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga
     6061 aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta acttaattaa
     6121 cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag
     6181 agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca
     6241 gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac
     6301 gatttggata cacattatga t
//
LOCUS       DAYYCM010000111         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000111 DAYYCM010000000
VERSION     DAYYCM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.116"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAY1_002714"
     CDS             725..811
                     /locus_tag="ACHAY1_002714"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8594650.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHAY1_002715"
     CDS             complement(932..1621)
                     /locus_tag="ACHAY1_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594651.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHAY1_002716"
     CDS             complement(1866..2780)
                     /locus_tag="ACHAY1_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8594652.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHAY1_002717"
     CDS             complement(2762..3142)
                     /locus_tag="ACHAY1_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8594653.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHAY1_002718"
     CDS             complement(3592..3879)
                     /locus_tag="ACHAY1_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8594654.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHAY1_002719"
     CDS             complement(3899..4123)
                     /locus_tag="ACHAY1_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8594655.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHAY1_002720"
     CDS             complement(4578..4748)
                     /locus_tag="ACHAY1_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594656.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHAY1_002721"
     CDS             complement(4763..5296)
                     /locus_tag="ACHAY1_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8594657.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHAY1_002722"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHAY1_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8594658.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYCM010000112         6008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000112 DAYYCM010000000
VERSION     DAYYCM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.117"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHAY1_002723"
     CDS             59..415
                     /locus_tag="ACHAY1_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8594659.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHAY1_002724"
     CDS             405..671
                     /locus_tag="ACHAY1_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594660.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHAY1_002725"
     CDS             830..1831
                     /locus_tag="ACHAY1_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8594661.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHAY1_002726"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHAY1_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHAY1_002727"
     CDS             3055..3300
                     /locus_tag="ACHAY1_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594662.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHAY1_002728"
     CDS             3398..3619
                     /locus_tag="ACHAY1_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594663.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHAY1_002729"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHAY1_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHAY1_002730"
     CDS             4761..5300
                     /locus_tag="ACHAY1_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594664.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT         2213 a          823 c         1071 g         1901 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatatttt
//
LOCUS       DAYYCM010000113         5717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000113 DAYYCM010000000
VERSION     DAYYCM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.118"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHAY1_002731"
     CDS             196..921
                     /locus_tag="ACHAY1_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594665.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHAY1_002732"
     CDS             911..1420
                     /locus_tag="ACHAY1_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594666.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHAY1_002733"
     CDS             complement(1490..2938)
                     /locus_tag="ACHAY1_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8594667.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHAY1_002734"
     CDS             complement(2938..3654)
                     /locus_tag="ACHAY1_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8594668.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHAY1_002735"
     CDS             complement(3635..3985)
                     /locus_tag="ACHAY1_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8594669.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHAY1_002736"
     CDS             4129..4902
                     /locus_tag="ACHAY1_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8594670.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHAY1_002737"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHAY1_002737"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5485..>5717)
                     /locus_tag="ACHAY1_002738"
     CDS             complement(5485..>5717)
                     /locus_tag="ACHAY1_002738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594671.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1660 a         1123 c          993 g         1941 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaata
//
LOCUS       DAYYCM010000114         5537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000114 DAYYCM010000000
VERSION     DAYYCM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.119"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHAY1_002739"
     CDS             complement(56..1561)
                     /locus_tag="ACHAY1_002739"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8594672.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHAY1_002740"
     CDS             complement(1653..2243)
                     /locus_tag="ACHAY1_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8594673.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHAY1_002741"
     CDS             2422..4068
                     /locus_tag="ACHAY1_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8594674.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHAY1_002742"
     CDS             4117..4836
                     /locus_tag="ACHAY1_002742"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8594675.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..>5537
                     /locus_tag="ACHAY1_002743"
     CDS             5315..>5537
                     /locus_tag="ACHAY1_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8594676.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFL"
BASE COUNT         1668 a         1006 c         1122 g         1741 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttat
//
LOCUS       DAYYCM010000115         5489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000115 DAYYCM010000000
VERSION     DAYYCM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.120"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(244..1263)
                     /locus_tag="ACHAY1_002744"
     CDS             complement(244..1263)
                     /locus_tag="ACHAY1_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8594677.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1260..1484)
                     /locus_tag="ACHAY1_002745"
     CDS             complement(1260..1484)
                     /locus_tag="ACHAY1_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8594678.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1481..1774)
                     /locus_tag="ACHAY1_002746"
     CDS             complement(1481..1774)
                     /locus_tag="ACHAY1_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594679.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1811..1948)
                     /locus_tag="ACHAY1_002747"
     CDS             complement(1811..1948)
                     /locus_tag="ACHAY1_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8594680.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1950..2381)
                     /locus_tag="ACHAY1_002748"
     CDS             complement(1950..2381)
                     /locus_tag="ACHAY1_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594681.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2395..2889)
                     /locus_tag="ACHAY1_002749"
     CDS             complement(2395..2889)
                     /locus_tag="ACHAY1_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594682.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2889..3338)
                     /locus_tag="ACHAY1_002750"
     CDS             complement(2889..3338)
                     /locus_tag="ACHAY1_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594683.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3342..3962)
                     /locus_tag="ACHAY1_002751"
     CDS             complement(3342..3962)
                     /locus_tag="ACHAY1_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594684.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3962..4870)
                     /locus_tag="ACHAY1_002752"
     CDS             complement(3962..4870)
                     /locus_tag="ACHAY1_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8594685.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4883..>5489)
                     /locus_tag="ACHAY1_002753"
     CDS             complement(4883..>5489)
                     /locus_tag="ACHAY1_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8594686.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
BASE COUNT         1504 a         1213 c          865 g         1907 t
ORIGIN      
        1 acctacgacc ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtactggtt
       61 gttgccacat aaagccataa acaatcaacc agtagaatgt gtggcaacaa acctgttatc
      121 gcatagcttg gagtgtgact attatgggtg atagtgaaga tatgcgataa catcactatt
      181 ttatcgaatg atttttatag ttgtcaatat agttatgtaa tgctcctcaa cgaggagcta
      241 tttttatcgt ttaggaatat ttaaatacca acgtttgtca tggaaatctt gcgcaccgcc
      301 tttagtgttt ccctctggat cattcgttgc ccgcatcatg acatagactt tcttattagg
      361 gaagttacgc atgttaaaag atacatgata gccaacattt ccagaagtat tataagcttg
      421 atttacatct gctctataaa ttccatcagc tcttactcga gctaattctt tcccagtatt
      481 gtaatccata atgaagatat actcgtattt atagttagca atgtgccatc cagctacatg
      541 caaatttgca ttttcgattt ctccaaactg atcaatgtgg gcgtaattcg ttccatctgt
      601 cagtgtagga ttagctgcac cagctcgtgt tggatcaatg acaggcttgt tttcagaagt
      661 tgttggattt tcatcggtaa atccatgagc taaatcatat gctaattttt ctttacttac
      721 gcccatttca gaaagataac cgtaaggatc tgtatgatcg ccccagatgt tttgtgttac
      781 ccataaatgc gatttgattc ccggttggtt ataaggagtg tccaacgtta atggaatacc
      841 atatttcatt gctgaatctc tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt
      901 tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga ctgttggcat tagcatacga
      961 accagcaccg tactgtacat aaccaggttg accgacttga taaacaattc cgccgtctcc
     1021 cacaatataa gcagtataag cgctagtcca tgaacgttgc atatactgcg cttcattgcg
     1081 tcctgttgct gtttcattag ccgtttcatg cagtaaaata tactgattat ttgctacttg
     1141 tgagctacct tcatttgcgc ccaaattaaa ttcattgttg atagtatagg caaatccgtt
     1201 aattggcaat aaaaaaagag ccgttaacag gctcatcgca gtaatagtaa ttttcttttt
     1261 catttgtttc ctccttcttc gctttcagcc gagaacattt tgtaggttcg atttgataca
     1321 cccaacacac tccctaaaaa cgcgccaaaa ccagtaatga tgacaacaca gatatctgtg
     1381 tactgccaat tgagcgcttt accaactaac cccacgaaag tagctagtgc gggaataatt
     1441 accagtgcga accattttag tacttcgaac gttttattat tcattttctt ctctccctaa
     1501 acaaagtttt gatttgttgc gtgtgttcca ccaatttttc tgtatgtgta tctaatcttt
     1561 catcgtgttt ctttagttct tcatgaatca tcaatcgatc tgatttgctc gattctaaat
     1621 ctttagtcag caaatctaaa ttgtggctta cttttgaaag agtctcagta attttcgaga
     1681 aagatgcagc aatcggtcta attactaata aaatcaaaga aacgatagcg gttattgatc
     1741 ctgctatcat tccccattcc cctaaattaa tcatgtgaca actcctttac cttaaataaa
     1801 aacgcatcaa ttaagatgcg ctcttatctt tattaatgat tttatctgct tcttcgtctg
     1861 taatgcataa tggaacgaaa accattactt gttcgttagt gaaacagccc cagtcataca
     1921 ttagtttgat atcttcatat gaatacatgt tacttcccac cttgtaattg ttttttgatt
     1981 tcttcgattt ccttggtatt ttgtacagaa gcaagcatcg tttttgaatt caactgcgct
     2041 aaactttctg acttttcttt caataagaca ttttcttgct tgattgctac attgtttagc
     2101 attgctttag aatttagctg tttcaattca tcattttctg ccttcagcgt ttcgtataat
     2161 gccttaatat tagccaattc atctacgcta tcaacagcat tccctttttt ctcttcttca
     2221 gtagctaact caacccactg ttttttatca aaatcaaatc ttggtctcca gttaggaact
     2281 ggtggtttaa tctctgtaca gttttcaggg atattttctt gattgttcaa aatgatttgc
     2341 tcgaatccgt aaggcttgat tgatttgtaa actactttca ttagatttcc tcccttaaat
     2401 tgaataagtg ataacaaatg aataagctga tccataactt gaatttcttc tccatttaat
     2461 ggctccgtct gcgccaatag atagttgagc actgttcaaa gtagaacggt ctattgaccc
     2521 aaccagttgt tcaaaactaa ttggtgtccg atagccttct ggaacagtta aaataaccga
     2581 atcattcccg ccactacttt ttccgtttaa agccacaaaa tatatagaaa ctgtttttcc
     2641 ttcacgataa agctttgctg tccctgtatt accgttagtg acaaccagat ttaccacttc
     2701 atatctgtta tcatcagtcg tcaaaacacg tttattattg attgttacag aacctgaaaa
     2761 attcttatgt cctgcaatgg cttggtctcc atctgtctgc accaatgctt ctttaccatc
     2821 aatagcttct acatgcgttt ttaagtattt agcaactccg tcttctttta attgaacaat
     2881 atcagccatt aaaccgtccc cactttctca aatgtaatat tcgctaatcc atcaagtttc
     2941 actttatcgg ttgctgacat taaacctgct gtcgtagttg tagcattacc tggatttttc
     3001 tgtgctccag ctgcaattcc atccaactta gttttatctg tggacgacat caacccattt
     3061 gctgtagttg tagctacagc tgtagttgtg gcatttattc caggatcacc tttatctccc
     3121 tttggtaaaa caaaattaaa tctagctgca gatgatgttc ctacattcgt aacagaagcg
     3181 gttgaaccac tagaaacggt tcctatggta attgttgctg cttggccagg atcgccttta
     3241 tctcccttca ccgttgttgg tttgccttct atagcattcc aatgagtttg tggataaacc
     3301 tgtacattgc tttgttttat ttttacgata tctgtcattc tctatacctc cccaattttt
     3361 tcaaaagtaa aatttggaat tctttcgttt gtgtaatttt ctgcttgatt tacagcttct
     3421 tggaattttt gatctacata tgactgattg acaccaccag tcccactacc acctgtagaa
     3481 ctaattgttc catcttcatc aatagtgatg ttttctccag cagtataatt tttcagtgaa
     3541 tcgagttttt cttttaactc cagcgaaaaa ttgacatcag attgtctgac tgcagagact
     3601 ttccctgtct catcaatcgt caataattca ccaactttta tgccacccaa acggtcgata
     3661 gtggcaactg gcaacacgta gtcaccttct ccactgttta aaattttttg atacatttct
     3721 gccgtgataa tgccgtcatc ttcttcactt gcatagggta attctgtgag aacattctct
     3781 aatcccaaat cttctttagt aatgacgacg gctccaattt ttccattaac agataaaact
     3841 gttgcttctc ctgctattat ttcttctaac ccaagaactg cggaagcatg tgtgattgga
     3901 aagaattggc gttgtacacc cgttttttca tccgtttcca tcattcgttt agttttagcc
     3961 attagatcac cccaactttc tcaagagtga acacattttg ctttggatca tcaacggttg
     4021 cgatgatcaa ggctccttct tcaatcggga atgctacagt tccaactttt tcgacttcat
     4081 gattatctga aaagagatca tcctgcaaaa tatcagtcac ttcaatctcc ccgtattcaa
     4141 tcgtgaaaag cgttgctcgc aatttctgat ataaatagtc catatctgcc agcaaacgct
     4201 ctgaaataga cgcatgacga actccttgaa tatctactcg cgcatccgtt agttcggcta
     4261 acatgactcc acctggatca atcgtcttta aaatatcctt gattgattca aaccatgcca
     4321 aatagtctga ttcttgtcct tctctccaat cttggaaact gtgttcctgt tcttgtctcc
     4381 aacgttcaaa ttcttctttc ctttcattca tccagtccgt aaaatcgcct ttattttcat
     4441 taataaagtc cgtcatatcc gcaattaaat cttcgattga ttgccaataa gaacccattt
     4501 ctccttctgt ttttgaaaca gcattaatga caaaataaga gaagtcttga gttgttccaa
     4561 tcaagtcttc tcctttaaat ataatgaaat tggctgtttg tcgatgtaaa cactgcatgg
     4621 aatatttatc aaaaatatag ttaattttcc cttttttagc atccacaatt tttgtttcta
     4681 attggactgg atatttccca cctacaactg attcaaaata taccttacat tctgataaat
     4741 cgtagggaag accattttca actatagtcg cttccataac ctctgtattc ttattgcctt
     4801 gtcgaacttg aatcatcccc acgtaattat agggttctgt tgtacttaat atgacattcc
     4861 actttgccat taaatcacct ccctattttg gtggtatgac aatggaagaa attgcacttg
     4921 cactataata ctggcgatct aactttccac aaatcatacc taactctgtg ttttgttcat
     4981 aagtttgcat acgaccatta gctaagcctc ggataacgcc agtatgtcca taagttccat
     5041 ctgcaaacca tgaacccact tgtccacctc ttgcccaatt aataattgca ccaactacta
     5101 attgatcata tctagggttt tgaatcactt tccaaccaac agcagaccaa tcataggcaa
     5161 taccaatatc agatgctgca gaagtatttc ctatcacatg agttaaacca tattttgttc
     5221 cagcacccat gccacaacca cccagatatc ctgaatattc tgcagacaag ccataacact
     5281 gaccattgcc aattcgttgt ccaatcaaag actctaaatg ttttagtcct gcttctccag
     5341 tagctcctcc tggttttaaa tctttaaatt tgttatacca gttaactgca taatcttgac
     5401 gttcaggatg tgtcgctgcc ggacgttcat agtttcgttc aaaagcatat gctgcttgtc
     5461 ttggatcagt acaggcctta aatccatca
//
LOCUS       DAYYCM010000116         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000116 DAYYCM010000000
VERSION     DAYYCM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.121"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAY1_002754"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAY1_002754"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8594687.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHAY1_002755"
     CDS             complement(1297..2133)
                     /locus_tag="ACHAY1_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594688.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHAY1_002756"
     CDS             complement(2317..3534)
                     /locus_tag="ACHAY1_002756"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8594689.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHAY1_002757"
     CDS             complement(3683..3844)
                     /locus_tag="ACHAY1_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594690.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHAY1_002758"
     tRNA            3917..4000
                     /locus_tag="ACHAY1_002758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACHAY1_002759"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACHAY1_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAYYCM010000117         5295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000117 DAYYCM010000000
VERSION     DAYYCM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.122"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHAY1_002760"
     CDS             <1..107
                     /locus_tag="ACHAY1_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8594691.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHAY1_002761"
     CDS             complement(176..904)
                     /locus_tag="ACHAY1_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8594692.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHAY1_002762"
     CDS             complement(917..2197)
                     /locus_tag="ACHAY1_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8594693.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHAY1_002763"
     CDS             complement(2338..2940)
                     /locus_tag="ACHAY1_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8594694.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHAY1_002764"
     CDS             3185..3391
                     /locus_tag="ACHAY1_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594695.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHAY1_002765"
     CDS             3703..4422
                     /locus_tag="ACHAY1_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8594696.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHAY1_002766"
     CDS             complement(4503..5186)
                     /locus_tag="ACHAY1_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8594697.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
BASE COUNT         1834 a          898 c          976 g         1587 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttc
//
LOCUS       DAYYCM010000118         5011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000118 DAYYCM010000000
VERSION     DAYYCM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.123"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..397
                     /locus_tag="ACHAY1_002767"
     CDS             98..397
                     /locus_tag="ACHAY1_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594698.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            662..1537
                     /locus_tag="ACHAY1_002768"
     CDS             662..1537
                     /locus_tag="ACHAY1_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594699.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1534..1752
                     /locus_tag="ACHAY1_002769"
     CDS             1534..1752
                     /locus_tag="ACHAY1_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594700.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1739..4366
                     /locus_tag="ACHAY1_002770"
     CDS             1739..4366
                     /locus_tag="ACHAY1_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8594701.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4381..4599
                     /locus_tag="ACHAY1_002771"
     CDS             4381..4599
                     /locus_tag="ACHAY1_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594702.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1966 a          622 c          994 g         1429 t
ORIGIN      
        1 aatacaaata aatacatttt tttacgcaat ttgtatttat ttgtacttat aaaggtagag
       61 gagttataga ataattaatt aaaaaggaga ggtttttgtg gaagcaataa aattaatgat
      121 ttcagaagaa caggaggaaa tgtttctaaa gcagttttat caaattgcta caaaagctat
      181 tgaaaaggct caagaaaatg cggggatgac aagggaatat ctcaatcaaa aagaaatggc
      241 ggaatactta aatgtgagtg aaaacttttt aaaaaatgct gtgagaaatg agggattgcc
      301 cgtagttgaa ttaggggcaa gaaaatttta ttcgaaaaag gcagttaatg agtttatgtt
      361 tgctcgaatg aaaagagggg aaaatattgg aagatgatga agtagaaatt ataaaaaaat
      421 gctttaaaaa aagaaaaaaa gtgttcccaa aagtttgcag actgggggaa cactcaataa
      481 taaagtgctg agaaaacttt atttatagct attatatcaa aaaagaaggt gaaaaggtgt
      541 tttcttctgg aaatgttgta gttgacaaac ttagagaaat aaattttgac ggaaatgtaa
      601 tccacacaca tggtacgatc aattaagaaa aaaaacgaaa aaaggtgttg agaagcccta
      661 tttgaatgca atagttatct tagcagaaat tacctattgg tatagaccta aaaaaatttt
      721 taacgatgat ggtcagctac ttggttatca aaaaaagttt cttgaagata ttttgcaaaa
      781 atcatataag caattaagca aaaaaacagg attatcagaa cgagctataa aagatgcaat
      841 tgtgtactta gagaaaaagg ggattatcaa aagggttttt agaaatattt attatgagaa
      901 aagagcagtt acattatcaa atgttatgta cattgtttta aatgtacata aattgataga
      961 aattactttt cctactttgg attgcgaaaa aaaggagtat atgccgaaaa ttcgtcagag
     1021 gggtatcgaa aaaaagagat atgtctcttt aaaaaaaggt agaggtatgt acaaatttgt
     1081 atatacgtat acaaagacta cttcacagaa catatcaaag actactctcg ttatggaaga
     1141 agaggatatt aatataaaga ggatttacag taagattagt gttctttatg acgcaaataa
     1201 cgaagatggt cgaaaattat ttcaagtgta tcactatttg agaaacgcta atgtagatac
     1261 agaagatatc agaaaaatca ttgaatattt atctgaaaat actcagtatc ttattgctga
     1321 gtgtattata aaacaacatg aagcatgctt ttttcaaaga aaaactgaag aagggttata
     1381 cgattatgta acatattttt tgaatggact tcagaaaaga tataaaagtc aatttttaga
     1441 aaatgtagag cctatagatc agttcaaaaa agctttcgaa atcaaagagt tacctgaaat
     1501 tactatgcat aattggttga aaggagaaga atcatgatta aaaatcgctt aatagatcaa
     1561 atgtggtgtc ttgatcaaaa tacagatact ctcgaattaa tcgagagtat ctgcttcaac
     1621 acagtagttc tcgatttaag agctgaaaat gacaattgtg taactcatat cataattaat
     1681 caaaaaatgg ctcagcagct ctctgagagc ctaagaaaat gggctgaagg agaaaactat
     1741 gaaagcaatt aaagaaaaaa tcgttagtag aaaaataaac tggttagcac tcagtgtact
     1801 tgttttaatg attagcgttg tttttacaag taatattgtt gaagctagca ttttcaattg
     1861 gtttgatgac acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct
     1921 aaaaaaatac aatggagatt tccttagtca gttcgggatt tggcttggat gggctgtggt
     1981 aaaaggcatg tttactgtga ctgattcgat tcaaaatatg attccagatg tgttggattt
     2041 gttcaatttc attgaaagta cagggctaaa taatgtttat caatcagtca taaatacaat
     2101 tgtcgttgga ttaatgatcc tttcattgat gtttgttggc tacaagatga ttactggaaa
     2161 aggaacaata gatttaagat ctgttggaat gaatatagtg atgtctgtag cgttaattct
     2221 tttaatgcca acaatgattt ctagcggtat tcaatttgcc aaaatatttt acaatgatgc
     2281 tactacgatt acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac
     2341 agatctggca tatattaata aaacagatca atactcaagc attgataaaa cggaggatcg
     2401 aaataagtta acacgaaaaa attttcaaca gacagattta acacaagtgt tgacggataa
     2461 agtgattgat aaattggaaa aagaaaatcc agctgcggat aatcttcgct atgaattagt
     2521 tgaaaattct aataatgaat ttgttgccac aaagttttct gataacttct tatcaacatt
     2581 ttctgatagc ttgaaatcag gttattatcg atatcaagca aatctttggg ggatttcgat
     2641 tgggttgaca gcgttggcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat
     2701 attagaactt gcatttaaac gtgtgttagg tgttcttgtt tttgcgacag atattgaaac
     2761 aggacaacgt tcaaaagttg ttttatccga tattttacaa tgttacttaa cagttggatt
     2821 tcaagggttt ggactatcta tgtttgctat gtttatcaat tttttaaatg ctggacaagg
     2881 tatctccaca aatattttta taaaaacaat tgcttatatc tgtgctgtgt ttgtgctgat
     2941 aaaaggcagt ggaacagtca tgagatactt tggtgttgat attggactca aagaaggcta
     3001 tgggcaactt gcttctgctt ttggtatggg cgcaatgctg tttagaaaag gatcaactgg
     3061 atttaatcgt gctaaaggaa gcggaaatgg aaatggatca gacagtggag aaggagaaga
     3121 tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca aaactggaag
     3181 agctttggga tatgcccatg aacgtggatt atcaggttta gcttcagatg gagctacaat
     3241 ggctagtgaa agagcaataa aaccatttaa gagtatgcgt gatatggcta atgataccaa
     3301 gaataagttc aaagaaggtt tagatgatgg tacagtgtcc gctattaata aaaatagcaa
     3361 acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt ccatgccaac
     3421 acgtctttcg gatagaaaag atggtgctaa ggttgaaaat gccgatagaa ttatgtctag
     3481 ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata atcctgtaag
     3541 tgcaattcag caaaaagttc aacaagatat tgaagaacgt aaaaatgcta tgcacggtca
     3601 agctaagtct gctgaagaat taattaatca gaagcgacaa gaagctaaat acacgcctga
     3661 agcaatgaat cgtgaggaaa tgttaaggaa acgtgtagaa gggcgtacag gagctaatac
     3721 ggacgaaaaa gaagcgttga ccaaagaaag aatccaagaa gctaaaaact caaataccgg
     3781 attagaaaaa ttggtcaaag aaaatctaca aaattctaca agtagtcaag gtggccgatc
     3841 agtagatgtc cgagaaaata ttcaaggttc attgaatggt catgtcggac ggaatgttga
     3901 tgtaagagag aatttacaaa gctcttcaaa tggtcaaggt actaagacaa ttgatgttcg
     3961 agaaaatgta caaaattcta caagtagtca agggtttaaa tcaatggatg taagagagaa
     4021 tgtacaaaat tctacaagta gtcaagggtt taaatcaatg gatgtaagag agaatgtaca
     4081 aagttctaca agtagtcaag gattcaaaac agtagatgtt cgggagaacg ttcaaaaaga
     4141 tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc
     4201 gaaaaaattt gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga
     4261 taagggatca aaaccaagac gaagatttac ctatgaagac aacgagttat tcagagatac
     4321 acttaatgat cctaatccgc ttttcgataa tctattaaaa aaataaaagg gggaattgtc
     4381 atgaaattaa ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa
     4441 tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt
     4501 caagaagaat taaagatgaa acatgagata aaaaacaact tgaaatattg ttatctagaa
     4561 aaaagtgaag agataatcca tgaattaaat ggcttttaaa aattgtattt gacatgaatt
     4621 tacgacaagg aattagtggg gattaagcga ttattctctt ccactttaga gaaataatgg
     4681 gttaaaaagc cagaatacgt agagcaatat tgacaaccta taaaggagct attgttgaaa
     4741 attgaagtaa caaaaagata aagggaggtt tacatctatc agattaaaag tgatagtaca
     4801 cccatgaaac ctgcaaaaga gttgaaccta atgtatatcg gtttaactct ttttatttgc
     4861 aaattaaaaa aatatcaccg aagtgacgaa tgaaactatg accaaattaa gcaagaaaat
     4921 atatcttgca aataaagtag tataaaaaga tattgataaa gccaatcaaa actttgaatt
     4981 tcaaacgtta gataatcaaa cagatacaaa a
//
LOCUS       DAYYCM010000119         4975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000119 DAYYCM010000000
VERSION     DAYYCM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.124"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1264
                     /locus_tag="ACHAY1_002772"
     CDS             44..1264
                     /locus_tag="ACHAY1_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8594703.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1261..3981
                     /locus_tag="ACHAY1_002773"
     CDS             1261..3981
                     /locus_tag="ACHAY1_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8594704.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3965..4909
                     /locus_tag="ACHAY1_002774"
     CDS             3965..4909
                     /locus_tag="ACHAY1_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8594705.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1847 a          817 c         1001 g         1310 t
ORIGIN      
        1 attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga aatttatcca
       61 tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg ataaaactgt
      121 acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat
      181 aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc
      241 attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga accatatacc
      301 tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt
      361 tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa
      421 aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga
      481 caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt tgtaccacgg
      541 ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa agatgcaaga
      601 aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct taaatgaaaa
      661 gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac aagcttctac
      721 atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga ttaaagtagc
      781 agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga caaaagaggt
      841 cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt taattgagat
      901 aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac aatcaggaga
      961 gctattagac tatttgttag aggaattctc agataggata aatgatttct ttatttggcg
     1021 tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt ctgctaaatt
     1081 gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga
     1141 agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat atcaagaaga
     1201 aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg aggatcagga
     1261 atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgac
     1321 ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc aacgatttat
     1381 caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa aaaggattat
     1441 acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc tgtatatggt
     1501 gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg
     1561 gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac gaaagagtta
     1621 ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca tttgagagaa
     1681 aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca agtattccaa
     1741 aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg ccaaaaatta
     1801 ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa
     1861 gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag
     1921 cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc cagacagcaa
     1981 gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag cactacaggt
     2041 cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta tcagcaaaca
     2101 aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat ggatcagcag
     2161 tcagccagat attattttta tttagagcag gagcaggaac taaaagatct ccagcaactt
     2221 caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga gactagagaa
     2281 acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg
     2341 gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag aaggattgct
     2401 gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca agagatgatt
     2461 gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa tcagtcagag
     2521 atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc aataatcatt
     2581 gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg tgcaggcatg
     2641 attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga tttctctgag
     2701 ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc tcaattagct
     2761 gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca gcaagaagta
     2821 tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact catgaaagaa
     2881 tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg gaaacataaa
     2941 agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga gtttgttaaa
     3001 gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct ttcccgtttt
     3061 atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag
     3121 caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata cagcgaacaa
     3181 ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa
     3241 caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac tccactattt
     3301 cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa agaaaagcaa
     3361 tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa
     3421 gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc tagccggcaa
     3481 aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc cgggacattc
     3541 ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt aagagcagca
     3601 ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact gaaaaatgat
     3661 acactgactt taacagacgg atatacagaa tttcccatct atgagttatc tacaggtacg
     3721 aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc taaccgttcg
     3781 atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg aaagtatcaa
     3841 ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat
     3901 aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt agaaggagca
     3961 aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat ttgtcctgat
     4021 caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca
     4081 agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg aaatcaataa
     4141 atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc
     4201 tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag agcctagttt
     4261 atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattgtt
     4321 gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta ataaatatca
     4381 aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg ccagtggatt
     4441 agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg aatatcctga
     4501 gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa aggtgattga
     4561 actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg gtcatctggt
     4621 attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga
     4681 agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag aatatggctc
     4741 acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca atatcgatgc
     4801 ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg
     4861 gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat
     4921 ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg ttttc
//
LOCUS       DAYYCM010000120         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000120 DAYYCM010000000
VERSION     DAYYCM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.125"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHAY1_002775"
     CDS             85..972
                     /locus_tag="ACHAY1_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8594706.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAY1_002776"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAY1_002776"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8594707.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHAY1_002777"
     CDS             2274..2720
                     /locus_tag="ACHAY1_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8594708.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHAY1_002778"
     CDS             2993..4345
                     /locus_tag="ACHAY1_002778"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8594709.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHAY1_002779"
     CDS             4516..4803
                     /locus_tag="ACHAY1_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8594710.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYCM010000121         4749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000121 DAYYCM010000000
VERSION     DAYYCM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.126"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAY1_002780"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAY1_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8594711.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHAY1_002781"
     CDS             complement(2066..3064)
                     /locus_tag="ACHAY1_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8594712.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHAY1_002782"
     CDS             complement(3290..3943)
                     /locus_tag="ACHAY1_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8594713.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAY1_002783"
                     /pseudo
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAY1_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
BASE COUNT         1392 a         1000 c          742 g         1615 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaactta cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctat
//
LOCUS       DAYYCM010000122         4698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000122 DAYYCM010000000
VERSION     DAYYCM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.127"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHAY1_002784"
     CDS             complement(543..2048)
                     /locus_tag="ACHAY1_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8594714.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHAY1_002785"
     CDS             complement(2045..2731)
                     /locus_tag="ACHAY1_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8594715.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAY1_002786"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAY1_002786"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8594716.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1327 a          964 c          777 g         1630 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttggctc
     4441 tataatttat gggactgatg aatcagaatt gattcttcag ccccattttt attttaggta
     4501 ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga
     4561 cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga
     4621 tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg
     4681 ttttctcacc tgctcccc
//
LOCUS       DAYYCM010000123         4625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000123 DAYYCM010000000
VERSION     DAYYCM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.128"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            994..1962
                     /locus_tag="ACHAY1_002787"
     CDS             994..1962
                     /locus_tag="ACHAY1_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8594717.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1979..2503
                     /locus_tag="ACHAY1_002788"
     CDS             1979..2503
                     /locus_tag="ACHAY1_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8594718.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2879..3160
                     /locus_tag="ACHAY1_002789"
     CDS             2879..3160
                     /locus_tag="ACHAY1_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594719.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3162..3578
                     /locus_tag="ACHAY1_002790"
     CDS             3162..3578
                     /locus_tag="ACHAY1_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594720.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4208..>4625
                     /locus_tag="ACHAY1_002791"
     CDS             4208..>4625
                     /locus_tag="ACHAY1_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphopain proregion domain-containing protein"
                     /protein_id="HFM8594721.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVD"
BASE COUNT         1765 a          806 c          746 g         1308 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caaacacttt agtgagtttt gtagaaagta
       61 cctttaaagg tactttcaca ttacgaacga tacgtccgta ggacgtactg aaagggtggg
      121 atttatgagc gaactaatgc aaaatttggc tagcaaaaaa agttaaaaaa tatatgctaa
      181 taatcgagcg aaatgaacac tttttcaagt gagcgaatcc gccatgacaa agctgaagca
      241 gtttatttca gctttgtcat ggcgtgtgcc tagactttta aaaaagtcgg cacacgagca
      301 aatatcttta tcaagctcta atttgcgttt taagacgttt gaaacgtcta agtatataaa
      361 tatataagag taggtttaag agtgcttaaa tcgaatttta gagggcattc agacaacttt
      421 agggttgtta gggcttgccc tgaccgcctg taaggcgctt taagaacaag ataagggatt
      481 tatactaaga ctgtcttaaa acgtctcaaa cgaaggctca tagcttctca aagagttcct
      541 tatgaacatt tttagcaaag gagccggaag cgactgcgca gtataatgtg agagcagagc
      601 gatcacacct ttttaagcga ctgtagggag taagattccc agttgagaaa aatgcgcgaa
      661 tctgtttgga gaaaaaagaa gtggtttttt aggcgggaaa ttttgtcccc ctcttttata
      721 atctcttttt aaacctcttt ttaaacctct ttaccgtcgg ctatatcctt actctcccaa
      781 gggaatgacg cttttatcta catcgttttg tctgtttatc tacatcgttt tgtctgttta
      841 tctacatcgt tttgtctgtt tatctacatc gttttgtctg tttgtacatt gaattagtac
      901 atcgacgatg ttaatataag atgtagataa acaggcattt tgtatttcat aacgtgtatc
      961 tcttgtgcga gtaagaaagg agctatttta aatatgtcta atgaaatcgt aaagtatcat
     1021 catgaattaa atacaattcc gttgcgaaaa tttacaccag tcgaaatgaa tttgttcttt
     1081 tctatcgtat caagaatgcg tgatgtaggt gataaaaaag tacaatttac attcgagcaa
     1141 ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa
     1201 acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa
     1261 cgtgaacgtt ttgttttatt taatcaattt aaaatcgacg gaaaatctga tattcctttt
     1321 gctgaaatac aagttcatga aaaagcctta cccttactta ataatcttga agaatgggtg
     1381 cgctattctc tccaacaatt caatgaatta gaaagcagtt attcaaaaac aatgtttcga
     1441 ttactaaaac aatacagaac caaaggtttc gcttattttt caaaagaaga ctttcatgaa
     1501 ttacttgata ttcctaaaag ttacaaccaa ggtacgattg atcaacgtgt gttcaatcca
     1561 atacgacaag aactaaccgc aatctttaaa ggtttaacaa tcaaaaagaa atacggaaaa
     1621 ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat aaaaaaatca
     1681 gacgacttta acaaacatgc cccaacaaaa gaagaacaaa acaaagcatt agaactagct
     1741 aaccaacaag ttcaatctga aaaagcacct acttcattac aagaaaaaat caaagaacaa
     1801 aagcgaaaat tggaactttt acaacagtta gaaaagttag aaagagaaca aaatacccaa
     1861 aataaagaaa ataatgcaca aaaatcagag ataaattccg atattccttc agaactaaaa
     1921 aaagacttat tagacaatct tcaaaactta ttcaaagatt gaggggtttt tagattttat
     1981 gacagaaaaa acaatcaaag aacttgctga agaattagga gttagtaaaa cagcaattaa
     2041 caaaaaagtt tctgacgaaa atcgaaaact ttggttttcg aaaatcggaa acaagtttgt
     2101 gattaatgaa accggacaaa aggctataaa atcaatgttt tttaacgaaa acgaaaactc
     2161 aaatcaacaa ctagttggcg aaaaaacgaa aaccgaaaac cacaaaaacg ccaaccaagt
     2221 tcccgaatta gaagtccttt ctctcctaga acaacagtta aatcaaaaag atatgcaact
     2281 acaagaaaaa gacgagcaaa taaaacaaat gcaaaaatta ctcgaccacc aacaacaact
     2341 caccctgcaa gccaaccaac aaatcgagaa actgcaggaa caactacagt tgacttatac
     2401 ggaagaatct tcggaaaata aatcaacgac tctttctgaa gaaacagaat cgatagaaaa
     2461 acaatcaaag gcggagaaaa aatggtggca attctggcaa taaaaccagc aatttaaatt
     2521 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
     2581 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
     2641 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
     2701 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
     2761 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
     2821 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
     2881 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
     2941 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
     3001 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
     3061 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
     3121 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
     3181 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
     3241 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
     3301 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
     3361 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
     3421 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
     3481 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     3541 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     3601 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     3661 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttggggcgct
     3721 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     3781 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     3841 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     3901 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     3961 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     4021 gtgagcgtct ctatccattg gtagaggggc tacttggtct agtcgacatt atgttaacta
     4081 gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     4141 ttatcgcata ttgtatattt taattatata attataggtg tataccactt aaatttaaca
     4201 caaagacgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc tcttttcctt
     4261 gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa attctaattt
     4321 caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa ctgattttga
     4381 acactccata acttcaattc ttttaatgga aaataacacc gaaactgata ttaactatac
     4441 tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt ctattttctt
     4501 tccagtattt tcagacgctg ataatacgat taaatatatt tataccgtca aaaaaaatac
     4561 aaatggagaa tattcaggaa gtatctcaca atttttagca aaagaacttc aaaatctagt
     4621 agata
//
LOCUS       DAYYCM010000124         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000124 DAYYCM010000000
VERSION     DAYYCM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.129"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHAY1_002792"
     CDS             186..2855
                     /locus_tag="ACHAY1_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8594722.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHAY1_002793"
     CDS             complement(2946..3560)
                     /locus_tag="ACHAY1_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8594723.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHAY1_002794"
     CDS             3680..4219
                     /locus_tag="ACHAY1_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594724.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHAY1_002795"
     CDS             4216..4554
                     /locus_tag="ACHAY1_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8594725.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYCM010000125         4563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000125 DAYYCM010000000
VERSION     DAYYCM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.130"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHAY1_002796"
     CDS             complement(<1..300)
                     /locus_tag="ACHAY1_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8594726.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHAY1_002797"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHAY1_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHAY1_002798"
     CDS             complement(1014..1304)
                     /locus_tag="ACHAY1_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594727.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHAY1_002799"
     CDS             1706..1942
                     /locus_tag="ACHAY1_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594728.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHAY1_002800"
     CDS             1949..3901
                     /locus_tag="ACHAY1_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8594729.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHAY1_002801"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHAY1_002801"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8594730.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1560 a          803 c          755 g         1445 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agc
//
LOCUS       DAYYCM010000126         4365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000126 DAYYCM010000000
VERSION     DAYYCM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.131"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACHAY1_002802"
     CDS             152..1075
                     /locus_tag="ACHAY1_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8594731.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACHAY1_002803"
     CDS             1081..2334
                     /locus_tag="ACHAY1_002803"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8594732.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACHAY1_002804"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACHAY1_002804"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8594733.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          939 g         1232 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattt
//
LOCUS       DAYYCM010000127         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000127 DAYYCM010000000
VERSION     DAYYCM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.132"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHAY1_002805"
     CDS             127..579
                     /locus_tag="ACHAY1_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594734.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHAY1_002806"
     CDS             593..1282
                     /locus_tag="ACHAY1_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594735.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHAY1_002807"
     CDS             1407..3758
                     /locus_tag="ACHAY1_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8594736.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHAY1_002808"
     CDS             3838..4233
                     /locus_tag="ACHAY1_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594737.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAYYCM010000128         4301 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000128 DAYYCM010000000
VERSION     DAYYCM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.133"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHAY1_002809"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHAY1_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8594738.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHAY1_002810"
     CDS             complement(2020..2922)
                     /locus_tag="ACHAY1_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8594739.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHAY1_002811"
     CDS             complement(2919..3611)
                     /locus_tag="ACHAY1_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8594740.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHAY1_002812"
     CDS             complement(3654..4295)
                     /locus_tag="ACHAY1_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8594741.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1322 a          920 c          753 g         1306 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag t
//
LOCUS       DAYYCM010000129         4255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000129 DAYYCM010000000
VERSION     DAYYCM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.135"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..493)
                     /locus_tag="ACHAY1_002813"
     CDS             complement(8..493)
                     /locus_tag="ACHAY1_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8594742.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(495..1166)
                     /locus_tag="ACHAY1_002814"
     CDS             complement(495..1166)
                     /locus_tag="ACHAY1_002814"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594743.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1429..2622)
                     /locus_tag="ACHAY1_002815"
     CDS             complement(1429..2622)
                     /locus_tag="ACHAY1_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594744.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2706..2906)
                     /locus_tag="ACHAY1_002816"
     CDS             complement(2706..2906)
                     /locus_tag="ACHAY1_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8594745.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3330..3563)
                     /locus_tag="ACHAY1_002817"
     CDS             complement(3330..3563)
                     /locus_tag="ACHAY1_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594746.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3570..4001)
                     /locus_tag="ACHAY1_002818"
     CDS             complement(3570..4001)
                     /locus_tag="ACHAY1_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8594747.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1047 a         1064 c          827 g         1317 t
ORIGIN      
        1 caactcttta tttacctttt ttgtatgctg cactgtcaat ccaagttcct aaattgttgt
       61 atccatcatc acttatataa tcatattggc catcaataat ttcgtcaaac cacaaatatt
      121 ctccatctgg tttttgccca attattttaa ttactgattg actagtagtt gtcatctggt
      181 taaaatcttt tattttacct atattaacgg ccaaaatagc atttcctcgc ttataactct
      241 cctggatttc atatttaaca tattctcgat caagagtttc tttacctatc agcaccacag
      301 tcactgatgt tccaattaat tgattatcta tccagttttt tatagcaata tctccattac
      361 gtttaacttt ctcaaattca gccttatcta caaaaccagc ttcctttgtt cctcgcaaag
      421 tccagctgtt tctaactacc attgctcttg ttacatctgc ataatggaaa ctaaaaaaaa
      481 cccttctcac catattaatc ctcctgaata ctatttatta aactgacaat ttgttcaact
      541 tttttatcta cttctccttc agtatctaat ttttctagcg cttgaaattt actttttaag
      601 gaatcatcaa aataatcact gaaattctct tgaatcttat caaacaaaac cttacttgca
      661 cctttggtaa atccaatcgg aactactata ttttcatttt gaacagataa ttcaaattct
      721 tcaatcattc catctgctgt aactatttca cctgattcaa gtttgtttcc aaaaacaaaa
      781 atactaatcc cagactctgc aataatgttt tctcgatatc ttctccactc atgaggcata
      841 tcaaggggaa gtggtcttaa agaaagtacg tcttgtatgt tattttttct taaatttctt
      901 ctctgcttta gaactcctga aacaatacta ttaccaattc caagtccgta acctgatact
      961 attctataac cggattcaga aagcctatat gctaatttct cgacaaattt taatcctttc
     1021 gattcagatg taaatggagt ataatcaaca gcacttcccg aaataaaaat agtttttctc
     1081 aaatactttt ttctaatact tttcaaaata tcagtaatct cagaataatc atctacttca
     1141 acaacgttta tcgaaaatct ggacaaatca ttgatttgta gtcttctttt tgtcagttca
     1201 taattatatc tctcttgatc aaaaaaatcc tcagaatcga caaaattttc ctttaaaatt
     1261 atatgggatt ttagattatc tttttagaag ttcttgaatg tcggtatagc ccgtgtttcc
     1321 gggcttttcc ggcatttttg atattgttag atacttacaa atgttcttat tacctctcac
     1381 catttccccc tcaaaagtag taaatggagt agtaaaaccg gccttgtgtc aggctgccag
     1441 ccgttccatt tctgcccttg cggaatcgaa tgtggcgtgt gcataatagt tcagcgtcat
     1501 ggtgatattg gaatgtccca taatgtactg caaggctttg gggttcattc ccgcattggc
     1561 taaccgggtg cagaatgtat gacgcatagc atggggcgtc gtcgttttcg gcaacgcttc
     1621 ttcatggctc ttgttgtact tcttcacaag cctgccaaac atcccgccat aatctaccgc
     1681 cgtcatggga tagccgttct gtttgagaaa caggaaattg gcatacccgt caataatgaa
     1741 cggctttgca cctttgcggt tttttatcac tcgctggaac gcctgataca cttcctcatt
     1801 catggggatt tttctgacgc cgctttgggt cttgggttcg tcaatatagt aaccgacgcc
     1861 ggtatttctc aaaagctggt ggctcacgat gattacccgg ttttcaaaat ccaaatcttt
     1921 gtccgtcagt ccgcacagtt ccgaaatgcg aagccctgtc cccagcagaa taacgattgc
     1981 gtcataatgc ttttggtaaa ccttatcacc ttgaataaag gacagaaaac tttcttcctg
     2041 tgctggtgta agaggcacct ttggttccgt atcgtcgtcc agaacgtcac taagctggaa
     2101 gtcaaacggg ttttttcgga tgcaatcgtc ctgtatggcg gtgtaaaaag ccgctttcag
     2161 ggaacgcttg tcgttattga tggtcttata agaaaggccc ttttctttca tccgaagcgc
     2221 ccattccttt gcgtcggaca gcttcacact gtcaatcggg cagctcccca gccgatcttg
     2281 ttccagaata cgcatcagcc gttcccggcc cagtttggtg ttgtgcttca cattgccccg
     2341 gtgcctgatc tgcttggcgt agagctggca gacggtcatt ttcttgccga tggtgtctat
     2401 cccgtcgtca aggtctttct ggatttcttt ctccttttcc cggagtgaaa tatcctcccg
     2461 tttacctgcc ggggtcttat ccgtaggcac taacttccat gagtaaacaa actgcggttt
     2521 tccaaaggta tcaacatatt tgtaagcata tcgtccgtct tttctctggc tctctccaga
     2581 ccgcaaaacg cgatttttgc tgtctcgtct tttttctgac atctcgtcat gctcctttcc
     2641 atgacggaaa gagccgcata tatgcttata aattattata acatatctgc ggcccttttt
     2701 cactattaga ttgtgtccaa agtgtcaatt attttctcaa actgcttgcg tttgatctgg
     2761 atgcggttgc cattcataat gacccagcca gaggccggat tttcctcggc cagcttacgc
     2821 aatttgtttt ctccgatacg gaaatattta gacgcttcct caatcgtcag cgtgtatttt
     2881 tcccaaatag gcacatcatg gttcataatg cagccccctt tcacaaattc aaaggtttgt
     2941 gtgatttagg ggcatagacg gacggctgct ggcacagctc cacgggactt tcacccccgc
     3001 gtggttctca cgcggcccta cccattgcct gcgacgcttt taacgctcgg actgtggcta
     3061 taagggagta tcatagtcct gcctgtacgt cgtcgcccgc aagccgccac gctcacattg
     3121 cggcgtggtg ttcctcgctc cgcttttccc gtagggaaaa gtatcatggc gcaccagccg
     3181 tccggctcgg cacagacaga atgtgtccgt atgccctatg atgcgccgcc gttatcttgc
     3241 gggcatattt atcaaggtgc gatcaggcca ccgggaaaac accgaaacgg ggaaagggta
     3301 agccgtttca tgctgccggg gccggttcgt cagttcgtga cgatagcgtg gatgagcttg
     3361 atttccagac ggcgcttcat ggcctcgtcc agacacacat gcggatagcc gttttcgtca
     3421 tagagcgtcc gggtacacag cttgttcatg taaccgctgt aatactgcaa aacgcgctct
     3481 acggctccgg catccccagc gcgggcggcc tcgatcaccg aaaggggcaa aagctcaatg
     3541 cccttgtagt tcgggttctt catccgcgtc taacctccct tcggcatcaa cgcctttagc
     3601 tgtttccgca gctccgataa ggtatttgtc ctgtgccgct gaacggcact ccgggacatt
     3661 cccacaaggc cgccgatctc accatctgcc atatccagaa cacagtgcag gattaaaata
     3721 ctctgctcca tgctgggcag cgcggcaaag gcttcggcaa caagctcatt gttgatatgt
     3781 aggtcatagc catgcgacga aaacgtaaag ctgtcgctgg ggtagtgatc tactgtacag
     3841 agcttgtcca aatctgcctg tgacaaagag cttagtgaca cttcacggtc acggcggcgc
     3901 ttcatttcag ccaagtagct tttggcctcg ttacgcagta ccgtcttgca aaaagcatcg
     3961 aacatgcacc gcgcttcata gtcgcggggg atagcatcca tcttctcacc ccctttctgt
     4021 gggggactta ggtggttctc tccctttccg ctaacaagac tttgacaagg ccgccagcta
     4081 cccgctaaat acctcctttt tgaaaatttc tcaaaaaatt tttttgggga cagccgggcc
     4141 gcaaaagacg acaaaaatcg cccggcaggt cgcaaaccta ccgggcgaga aaatgctttg
     4201 ctatgctgct gatttacatg gtatagtaca tactcataac cggaatagcc ctgtg
//
LOCUS       DAYYCM010000130         4252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000130 DAYYCM010000000
VERSION     DAYYCM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.136"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..572
                     /locus_tag="ACHAY1_002819"
     CDS             216..572
                     /locus_tag="ACHAY1_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8594748.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(813..1817)
                     /locus_tag="ACHAY1_002820"
     CDS             complement(813..1817)
                     /locus_tag="ACHAY1_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8594749.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            1983..2834
                     /locus_tag="ACHAY1_002821"
     CDS             1983..2834
                     /locus_tag="ACHAY1_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8594750.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2851..3096
                     /locus_tag="ACHAY1_002822"
     CDS             2851..3096
                     /locus_tag="ACHAY1_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594751.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            3283..3987
                     /locus_tag="ACHAY1_002823"
     CDS             3283..3987
                     /locus_tag="ACHAY1_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594752.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1414 a          797 c          745 g         1296 t
ORIGIN      
        1 agtcaactaa gagcactcgt ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa
       61 aatgtgtttt ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa
      121 caaaatgagg aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt
      181 ctatttcgtt aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg
      241 tcgttttagg catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg
      301 aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg
      361 gagagaaatt cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa
      421 aacaggtgac gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac
      481 tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc
      541 ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa
      601 aaagaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
      661 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt ctcacgctct
      721 ttttttgtta tattcgtctt ttccgatcct tctcttgaca caattcattt aactgatcac
      781 tggtacttga acacctaata ttctagtcat ttttataaac gattcaatac ttcatccaca
      841 tttttgatca tcacggatat cgtaccatcc ggattattca caaattcaat caaatctggg
      901 ttgcgatata catctactgg tacgatcagt tcgatcccgt ttgatagctt gaatttttgt
      961 tttccaaatt ttttctcaga aatttcttgt acttctttta caggaggtgc ttctgggaca
     1021 aatccttgct cttgtacttc ttctttaaaa gccatttttg ccgtgatgtt ttccttgaag
     1081 actttttccg caatcatctc atgatccaac cgaccttttt cttctatcgt atcatagaca
     1141 gcttctttca aatcagccat gatatcaaat tcttctgtct caaatttttt tccaagatgc
     1201 ttggctactt tttggatcac ttttacattt tcttctaaag aaggtgcagg aacactttcg
     1261 atcactcttc cagaaaaata aagctctttt ttgccagaaa actcatattt tttttcaatt
     1321 aattcgtagg tccaatcagt caaattcaca gcgaatgctt catctgcttt ttgtgttttc
     1381 gcccctagaa ttgctcgatt caaaatcaag cggttgtcga tccctgcttc gtctgcttca
     1441 acaaagtgag tgtaagcctc acgataattg actttcaata aagataagta catcactgta
     1501 tctaattcat acaagacaac aaatacatct gcactaggtg cttcttcact ttcttggtag
     1561 atatcgaacc accgctgaac gattgcttca cttgcttcca caaaattttc ttgagcgatt
     1621 ttaagtgatt caacgatcat gctttcttct gctagttgtc cagtttttgt ttgagcagaa
     1681 gagatttttt ggatcttctt ttgaagaaaa tcacgaatat attcttttgt cagatccagc
     1741 tctttttgtg aaaagactgg agaaccagtt tctctgtcaa taatatgcag aattgctttt
     1801 tttaaataga tatccatttt ctcattccct tctgtcttag tttacggaaa aaaaatcaaa
     1861 aataccagaa tcaaaatcat tttcttttaa acaaaaattt caagagtaga aacaaaatgg
     1921 attctttttt tcgaaaaatg cgctacactt atagttgaac gaataaaaaa ggagctgaaa
     1981 gtatgaattc attaacaagc acttatcgtc tatcaaatgg ttacgaaatc ccggtagtag
     2041 gattcggcac atggcaaaca cctgatgggg atgtcgctgt ttcctctgtt aaagaagcgc
     2101 tggctgcagg ttatcgccat atcgatactg cccaaggata caagaatgaa gaaagcgtcg
     2161 gccaagcgat taaggaaagt ggaattccta gagaagagat attcttgaca acaaaattat
     2221 ggaatgcaaa ccacagctat gaacttgtga tgtcttcttt tgaagaatca ttgaagaagc
     2281 tccaaacaga ttatttagat ctgtttttga tccactggcc aaaccctgtt gcttttcgtg
     2341 ataattggga acaagcaaat gcagatacat ggcgagcatt tgaagaactg tatgaagcag
     2401 gtaaaatcaa agcaatcggt gtgagcaatt tcttacccca ccatctagat acactagcaa
     2461 aaacagcaaa aattatgccg atggtcaatc aagttttcct tgcaccaggc gaattacaga
     2521 caactgtagt tgagtatgca aaaaaacatg atatgatcct cgaagcatat agtccattag
     2581 gcactggtaa aatcttcgat gtgcctgaaa tgaaacaaat cgctgaagca catgacaaga
     2641 cgattgcaca agttgctttg cgttggtcgc tgcaacacga attccttcct cttcctaagt
     2701 cagtgacacc aagcagaatc aaagaaaata ctgaattatt tgattttgaa ttaacagaag
     2761 aagaaatgaa acaaatcgat cagttagatg gtgtagtcgg caaagcaaaa gacccagata
     2821 ctacccaatt ttaagtcgaa aggtgatttt atggatccaa tcgaacgctt gaattctctc
     2881 tcggaagaag ttacccagac ttttcattcc gattttgtct ttttgatcga tgcagagaaa
     2941 atccaacatt ttcctgcaag aaactggaca catgatcaaa tcatcgaaga actgaaaaaa
     3001 cggttcgatc attcgctaat ggtcaaaccc tggcacgaac atgaagtcat ttattcacct
     3061 gaactacctg tttttgcctt gataccgaaa aaatagaaag aatggaacgg attatgtata
     3121 tatacataat ccgttccatt ttttattttt ctttcaaaaa acgggaaaat acgcaaattt
     3181 ttctgttgta atttgcgtat acgcaagttt ttttgcatta ctccttttca tttggctgtt
     3241 tttttcttat aatcgaaagg gtatgagcga aggagagaac atatgaagaa aaaacagaaa
     3301 caaaaattat taaatcaatt ccgtccttca ttggatggtg taagaggaca attattccgt
     3361 tctctagaag aagagagctt gcgacgttat ggtttgccgt tacaagtcat gcttgatccc
     3421 aaaaaccgtc aaatcttaac gatcggatta gctgggaaaa caaatgaaga tacacggatc
     3481 aacgtgccaa tcgatgacaa cttcaatact gtattaaaga gaattcgttc aggagaaaac
     3541 gggattttcg aacgattccg tgacaatctt cttattgaaa tcgtgtctta ctggaatgat
     3601 caacgattga aaaacgatga accaactgcg caagctgttg cgacaccagt tacagaagaa
     3661 acagtcagtg tgccagaaga aaaaacggaa aaaccaaaag cggacacaac tgaaaataca
     3721 cgctcaactg atacgaatgc cgctttaagt tttgctgatt tttcaaaagc cgttgctgag
     3781 tatcctaaat tttacacaga acaaaatgaa catgaagtag ttattaatga aaaaaatgct
     3841 gatgaacctc gtaaattagc aactatttct atgactactg aaaatgagtt cgttatcgaa
     3901 aaagcactcg aacggaaata caaagtcaaa ttgactttga ttcctttgat cgaacaattt
     3961 gcagcgacag cgattactga tcgataaaaa ttctatttgt cataaaaggc tgggatttaa
     4021 aatcccagcc tttttagtca tataataatt tctacagcaa gtaaaaagaa attccttcta
     4081 acatcaggct caaatatcag cttgacctgt ccaacaaatc acctatgatc ctacaatgtt
     4141 aggcgacttc tcttctacct gaagatatct tggagttgcc tgatttctta agatgcctaa
     4201 ccttaactat aaaaaattct aataataaac atattttctg atagagaaaa aa
//
LOCUS       DAYYCM010000131         4211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000131 DAYYCM010000000
VERSION     DAYYCM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.137"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..715
                     /locus_tag="ACHAY1_002824"
     CDS             14..715
                     /locus_tag="ACHAY1_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8594753.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1287..2303)
                     /gene="tsaD"
                     /locus_tag="ACHAY1_002825"
     CDS             complement(1287..2303)
                     /gene="tsaD"
                     /locus_tag="ACHAY1_002825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8594754.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2300..2764)
                     /gene="rimI"
                     /locus_tag="ACHAY1_002826"
     CDS             complement(2300..2764)
                     /gene="rimI"
                     /locus_tag="ACHAY1_002826"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8594755.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2771..3313)
                     /gene="rimI"
                     /locus_tag="ACHAY1_002827"
     CDS             complement(2771..3313)
                     /gene="rimI"
                     /locus_tag="ACHAY1_002827"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8594756.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3297..4019)
                     /gene="tsaB"
                     /locus_tag="ACHAY1_002828"
     CDS             complement(3297..4019)
                     /gene="tsaB"
                     /locus_tag="ACHAY1_002828"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8594757.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1232 a          909 c          729 g         1341 t
ORIGIN      
        1 agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca
       61 acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct
      121 tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta
      181 tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta
      241 ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac
      301 ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta
      361 ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc
      421 taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga
      481 ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg
      541 tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac
      601 acgtgaaact gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga
      661 caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag aataacgcga
      721 acaaaaataa gcagtgaccc tgatatttat cagggtcact gcttattttc ctccctaaaa
      781 cttctgaaag atccggcagt tttagatcac tgggcaagac tcaaagattt tctatgtgtg
      841 tttcagttat gtaatattca ctgtttgcaa aaaatactga tagattaact agaggaagcg
      901 tcttgtcctc atccgaccta gattattcga tattcctcga caataaacga ttaatcagtg
      961 agggcaatca gaggaacgga taatgatgaa taagctgtaa aaaatagggc tttgacaaaa
     1021 gcgtgtcaca gcccttattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag
     1081 tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt
     1141 aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat
     1201 aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga
     1261 tgcagggcag atttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc
     1321 aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt
     1381 gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta
     1441 gttgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct
     1501 acaatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca
     1561 tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc
     1621 tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca
     1681 taattatctt catggatcat cgctcgtgga aaatggtaat tatcttttcc ctgatgagct
     1741 agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca
     1801 taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct
     1861 tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg
     1921 aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc
     1981 ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg
     2041 acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct
     2101 tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact
     2161 tcaggaacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat
     2221 aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc
     2281 gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga
     2341 tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt
     2401 ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca
     2461 gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc
     2521 cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt
     2581 atccaataaa gccacagatc tggttatctt caactaaaag cagataatca gagtgaggct
     2641 gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt
     2701 cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa
     2761 tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt
     2821 cagtgtaata tctttttttg atcgttgttg cttgaaaacc taacttccga taaagtcttt
     2881 gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag
     2941 caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc
     3001 gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa
     3061 tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg
     3121 atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca
     3181 gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt
     3241 cgattgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa
     3301 attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct
     3361 tctacccgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcagctaaa
     3421 gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc
     3481 atcgcactag gcaatatttc ctttatttca tagaagaatt tgtgacaatc actcccgacg
     3541 aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt
     3601 acatcttcaa gtacaatttc taatctattt tctttccagc ggtatgcccc agcgtataca
     3661 ttttttcttc gagcatcaaa catgggcaca ataattttat ccacaccgat gcaattagcc
     3721 gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt
     3781 tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg
     3841 attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc
     3901 atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg
     3961 tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata
     4021 tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg
     4081 aggatgaaaa taccaaaggc aaaactagta caatgataca tgattatctg ctggcgggca
     4141 aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc acttcatttc cccacagctt
     4201 ttccatcttt t
//
LOCUS       DAYYCM010000132         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000132 DAYYCM010000000
VERSION     DAYYCM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.138"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACHAY1_002829"
     CDS             complement(<1..1155)
                     /locus_tag="ACHAY1_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8594758.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACHAY1_002830"
     CDS             complement(1158..3407)
                     /locus_tag="ACHAY1_002830"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8594759.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACHAY1_002831"
     CDS             complement(3504..4007)
                     /locus_tag="ACHAY1_002831"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594760.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          743 c          580 g         1433 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attacat
//
LOCUS       DAYYCM010000133         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000133 DAYYCM010000000
VERSION     DAYYCM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.139"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHAY1_002832"
     CDS             85..1434
                     /locus_tag="ACHAY1_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8594761.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHAY1_002833"
     CDS             complement(1562..2152)
                     /locus_tag="ACHAY1_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8594762.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHAY1_002834"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHAY1_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8594763.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYCM010000134         4030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000134 DAYYCM010000000
VERSION     DAYYCM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.140"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..181
                     /locus_tag="ACHAY1_002835"
     CDS             <1..181
                     /locus_tag="ACHAY1_002835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594764.1"
                     /translation="GIWLANIELAKPFSVFCRKGPYSHFHITNDTSIGRIKRVGLMSK
                     LKQNLASNPSKKYTY"
     gene            958..1926
                     /locus_tag="ACHAY1_002836"
     CDS             958..1926
                     /locus_tag="ACHAY1_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8594765.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1936..2424
                     /locus_tag="ACHAY1_002837"
     CDS             1936..2424
                     /locus_tag="ACHAY1_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8594766.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            2720..3001
                     /locus_tag="ACHAY1_002838"
     CDS             2720..3001
                     /locus_tag="ACHAY1_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594767.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            3003..3419
                     /locus_tag="ACHAY1_002839"
     CDS             3003..3419
                     /locus_tag="ACHAY1_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594768.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1502 a          702 c          671 g         1155 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtccata ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga acaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt tatttaatca atttaaaatc
     1261 gatggaaaag ctgatattcc ttttgctgaa atacaagtcc atgagaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggttcgatat tctctccaac aattcaatga attagaaagc
     1381 agttattcca aaaccatgtt tagattatta aaacaataca gaaccaaagg aattgccaat
     1441 ttttcaaaag aagactttca tgaattactt gatatcccta aaagttatag agaaagtgat
     1501 ataaataaaa aaattataaa gcctattcgc caagaactaa ccgcaatctt caaaggtttg
     1561 acaatccaaa agagatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgctccaac caaagaagaa
     1681 caaaataaag ctttggaact agctaatcaa caggttcaat ctgaaaaaga acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaaacga aaattagaac ttttacaaca gttagcagaa
     1801 ttagaaagaa aacaaaatac cccaagtaag aaaattaaca aacaaatagc agataacaat
     1861 tccgatatta ccccagaatt taaagaaaac ttactagata atctccaaaa cttatttaaa
     1921 gaataagggg tttttatgac agataaaact atcaaagaat tagcagatat tctaggtgtt
     1981 tcaaaagata aagtgaagta tcaactgagg aaattaccta gtaattttac ctacaaaaaa
     2041 ggaaatatta cctacctgaa aaaagaagca gtaacaacaa ttacaaaggt tttatcaggt
     2101 aatgaatcgg gtaacttacc tagtgaatta cccgattcat tacctaccga ttacctactc
     2161 aaggagattg aaactaagaa taatcatatt gaagaaaaag ataggcaaat tgaaaaactt
     2221 caaatagctt tagaaacgca gcaaaaatta ctcgaccagc aacaacaact caccctacaa
     2281 gccaatcaac aaatcaaaaa acttcacgaa caactccagc taacctatgc agaagaatct
     2341 tcagaaaaag aacccctttc tgttactgaa gaaactcaaa aaggacaaaa caaggagaaa
     2401 aaatggtggc aattctggca ataacccaac aatttttatt cataaaaatt gttgggttac
     2461 tttctttttt tgcttttaca agcatagcga catgatctca cgtcgctatt ttttaaagag
     2521 cataaggaaa cgcggtatca ctttcttacg aaaggtgcta ggtaggctgt gcctaccacg
     2581 tttctaagaa aaaaatttgc tatcactttt tcgtcattgt ggatagctta tgtatagtgt
     2641 ttcggcaaaa aacagctata caaatactat atttgtatga tatacataaa ttatacacaa
     2701 atgcgaaagg aattcttcta tggacatttt agttcggaat attgatccaa aagttatttc
     2761 agaaatagat agacgttgcc aagaactcac aacaaaaact ggcaaaaaat ggagccgtaa
     2821 tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag gcacttaggc attacaaaaa
     2881 agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa atactgaaac
     2941 attgaacgac tatgttcata caaacaacca actcattgaa acacttatcg gaggaatata
     3001 acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac attgacgttt
     3061 tagattccat catcgaaaaa aatccagata tcaataacta ctcggaagcc attcgttttc
     3121 tatgtatgag ttatgaacct aatactaata cgacaactac cagcaaccaa gcaaaactaa
     3181 atgcgatggg aaaagaaatt tctattatgt ccgaaatcat tgcagaattt gctgatttat
     3241 atctaaaaga tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag
     3301 aaaaaattga aaatcaactc aatccctccc gacgaaatca acccaaaaat cgtcaaaaag
     3361 aaaccaatca aacattcact tctagaaatt ttgacccaac aaactttcta aaaagatgaa
     3421 aaagaaaccg aaaatttttt ctatttctca gtttcttttt tagggggcag tcttccagca
     3481 agatttgcaa gttaccggta acttgcacaa aaaaagaaaa atccgcttgt gcgaatcctt
     3541 ctttttgcat tttggggcgc tgccccaatc tccaaatatc ggttatttaa tagcgtggta
     3601 actcaaaaaa atagaacgta ctctgaaata aaaaaagact gctctaacga gcagccaata
     3661 aaaaataatg agcgtttatt cttgaaaaag cagcgcgctt atgaagcgct gccaaaaatt
     3721 taccatattt tatgaaaaat aacaagtttc taaaaaggaa tcggttaaga ttcacaaaga
     3781 atcgtaaaca gacaatagcg gaaaatcaag cttccgaacg taccttttag ctagaccaat
     3841 gaaaaatcac aaagtgagcg gctggatccc ttggtacagg cgttacttgg tagtatacac
     3901 attatgttaa ctaacgctat aaataacgat tatgttccca aaacaatatt ttatgaacta
     3961 taacaaaaat ttgttatcgc atattgtata ttttaattat aggtgtatac cacttaaatt
     4021 taacacaaag
//
LOCUS       DAYYCM010000135         3992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000135 DAYYCM010000000
VERSION     DAYYCM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.141"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            741..1781
                     /locus_tag="ACHAY1_002840"
     CDS             741..1781
                     /locus_tag="ACHAY1_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8594769.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2605..2937
                     /gene="traE"
                     /locus_tag="ACHAY1_002841"
     CDS             2605..2937
                     /gene="traE"
                     /locus_tag="ACHAY1_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8594770.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3556..3897
                     /locus_tag="ACHAY1_002842"
     CDS             3556..3897
                     /locus_tag="ACHAY1_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8594771.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1460 a          510 c          719 g         1303 t
ORIGIN      
        1 cttgtacaaa tttatctgat attctcttat taagaactac ctaatttggg tagttcttat
       61 tttttacatt ttaagtagta tttaattgca atttggtagt atttttttat tatcttattc
      121 ttagaacggt agtacttaat ataaatatag gtagtatcta atttaagtat taatggtagt
      181 atctaaatta acaggtagta tgtaactact ttaaagtaat ttttttcaga ttaacacagt
      241 agtatcttat taaataagtt aattttaatg gtagtacata attattatat atatctgaca
      301 ttggtagtac ttattgtatt ttttagtagt atataatcta ttgttttcta gcctaaagcc
      361 ttgtcttata agaataatat aatatatact actgaaataa ggtagtatat attactagta
      421 atggtagtat gttttatatg cataaggtag ttcttaattt attttttgtt ttgacactgg
      481 tagtacttaa ttatctttaa ttttaaaact aaattttatt catacgtttt tctggtagta
      541 tttaattata aataatattt atttaaagta gttcttaatg actttaaagg tagtacataa
      601 ttcattttat ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca
      661 ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac
      721 gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca
      781 acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga
      841 aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca
      901 aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta
      961 gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg
     1021 gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata
     1081 gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg
     1141 atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg
     1201 acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc
     1261 aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag
     1321 acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag
     1381 acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat
     1441 taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta
     1501 ttcataatgc aaagaaaaaa gctgaaatag aaagtgatat ttcgatagtt tttgaagaac
     1561 tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc
     1621 aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa
     1681 attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg
     1741 caagctcccc aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga
     1801 ttgttgcttc atggagtaac attaaaatta aaaatttttt attattctaa ttatattacg
     1861 aatattacaa aataatagct attttaatag aatttataag ttaaaatatt atgataaaaa
     1921 tcacaaacgt agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga
     1981 aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga
     2041 taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa
     2101 tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga
     2161 ttttcgtttt gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag
     2221 gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt
     2281 ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac
     2341 agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt
     2401 acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat
     2461 gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc
     2521 atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt
     2581 taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt
     2641 tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc
     2701 caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag
     2761 gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta
     2821 aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga
     2881 agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat cggctgaatt
     2941 gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact
     3001 tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat caacaattcc
     3061 cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg gtcaatcgag
     3121 taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag cgcagagtaa
     3181 acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat catgagcagc
     3241 tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat
     3301 ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac
     3361 gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg
     3421 ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga
     3481 acaagtcctg aataaagtga aaaaattgat ataaaacata gagaaaatga aatttacgta
     3541 atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt
     3601 aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag gggcggaaat
     3661 tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact
     3721 gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa
     3781 atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg
     3841 agtgattata ttgtcagctt tgaaatcatt gaatttgtag cggataaaga agaataaaaa
     3901 ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg
     3961 tagtattacc ttaggcattg agtaaggaat tt
//
LOCUS       DAYYCM010000136         3949 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000136 DAYYCM010000000
VERSION     DAYYCM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.142"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..555
                     /locus_tag="ACHAY1_002843"
     CDS             <1..555
                     /locus_tag="ACHAY1_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8594772.1"
                     /translation="TIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELA
                     VSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFS
                     VSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRV
                     FKKYAGVSPKQYQLGLVDNFNNIS"
     gene            572..1006
                     /locus_tag="ACHAY1_002844"
     CDS             572..1006
                     /locus_tag="ACHAY1_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594773.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1623..>3949
                     /gene="esp"
                     /locus_tag="ACHAY1_002845"
     CDS             1623..>3949
                     /gene="esp"
                     /locus_tag="ACHAY1_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8594774.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1443 a          557 c          752 g         1197 t
ORIGIN      
        1 acgattcggt ctgaaaaaaa ttattctatc attgtttttg aaaagttagc acaaacgtct
       61 ataacattag gaatggatat aatagaagcg tatcaaagta gagatgcttt gatacaagaa
      121 aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga gggattctgg gattgtttat
      181 tatactaagg aaatcggaaa aactaagata gagcatcttt ctcccttaat ttcttctgtc
      241 gttcaattca tcggcttaaa tatctataaa agaattactg taaaggaaat agctaattat
      301 ttttctgtaa gtgaaactaa attaagagaa agctttaaga atgaaatgca tattacgatt
      361 tataattata tctctaagcg aaaaatttct acggctaaaa taatgttgaa atcaaaccat
      421 actatcagtg aagtttcttt aggtttagga ttttcagatt cttcacactt ttcgagagta
      481 ttcaaaaagt acgcaggtgt atctcctaag caatatcaat taggtttagt ggacaatttc
      541 aacaacatta gctgagaggt tttattttat tatgagacta tatcatttac gtagtttgga
      601 ggagttgagg aaattggatt ttaacaaaaa atttttttgg gaaatttctg atttgagttg
      661 tttcgtagaa tctgagcgtc taagtaatgg taataatagt gtgttaaatg aatatgacaa
      721 agtagtaatt aataaagtaa aaagtttatg tgaagaagct agagaatcta tcagtcatat
      781 taatggtagc aattttaaac aaacattatt taatctgatt aagctagatg ctaaaatatc
      841 aagttattta ttctttttga tacatgatga atttatggat tatcaaaaat tggatcagtt
      901 aattgaaaga gaaagctggg aagattatta cgtgggtcta actttttcag aaaagttaaa
      961 caactacaag ttaatagatt ataagtattt gagtgaaagc aattaattat tcatgtagag
     1021 tactagacac tttaaatatg gacaattcaa ttataaagaa aaagaatcaa taatttaatg
     1081 aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact
     1141 ccaatcattc gtttaagatt ctaggaatca ttcagaaatg ctgtgaaacc gatactttat
     1201 aattataaag aaggtgattt caaattccga agaaggaatt tgaagtcacc tttttactta
     1261 tgtcacagtc tcattcagta atgcgggata aggctcctta ttccgttatt tgaatactct
     1321 gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac
     1381 ttaatttcag aaagaataca tacatatctt tttatatgtt ttcagctgct gaatggttaa
     1441 aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt tttttgttgt aatgttcatg
     1501 aaattctcta agtaaattag ttaacataag gttagtaaga ttcctattct gtgaataata
     1561 cgtatcagat ttatctttta ttcttactag gaaagtaatt attaagggag ttgatttgaa
     1621 taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa cgagtattga
     1681 agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttgattggt gttggtcttg
     1741 tcttgggaac aactgaacta gttaaagcac aagatgaaat ttctccaagc agcccacttg
     1801 aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat acttttcagg
     1861 agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt cctcaagaac
     1921 taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga aaacataata
     1981 ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga ttagctaaat
     2041 atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga cggacctttg
     2101 taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat tatattcgtg
     2161 caagtgatgg attatttgct ggggcagaaa caaccgatac gcaaaccgct cctaatgggg
     2221 taattacgtt tgaaaagagc ctagatgaaa tttttaaaga gataggcata gacaatctca
     2281 agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat gataaaattg
     2341 taccgggtat tgatagtacg ggctactttt taacagattc agatgatttc tataatagct
     2401 tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg
     2461 agaaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat ggagctataa
     2521 tcttagatca taagttaacg aaaaattata atttctcata ttcatcttcc gcaaagggca
     2581 cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta gcgggtatac
     2641 agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa
     2701 aagtagctga tttagcaatc gagcgacggg aaggtcatga aaattatggc ataggttcaa
     2761 taactaataa tgatttaact aaacttatcg attttgctaa tgcaagtcca cgtccagtcg
     2821 ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa gatatgaaag
     2881 caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt gattcttggt
     2941 tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat tattatttgc
     3001 aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac acgaatccat
     3061 atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt aaggggaaag
     3121 tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat aaagaaggta
     3181 ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca atttcagata
     3241 ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa
     3301 aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc gttattaagg
     3361 aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca gatgctaaag
     3421 aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg aaaacagagc
     3481 ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag atcggaaatc
     3541 gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg gaaaatgatg
     3601 caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga aaacctacat
     3661 ttgataaacc agatgttcct ttagctaaag atgccttttc aatttcagat gtctataata
     3721 agtacttcgg taatgcaagt gttgatgcaa acactggtat tgttacattc actccagcta
     3781 aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt gtttaccaag
     3841 atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt tatgaaaatc
     3901 caggagaaaa cattcctgca ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DAYYCM010000137         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000137 DAYYCM010000000
VERSION     DAYYCM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.143"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHAY1_002846"
     CDS             complement(718..3867)
                     /locus_tag="ACHAY1_002846"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8594775.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAYYCM010000138         3792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000138 DAYYCM010000000
VERSION     DAYYCM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.144"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..287
                     /locus_tag="ACHAY1_002847"
     CDS             <1..287
                     /locus_tag="ACHAY1_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594776.1"
                     /translation="HAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(413..1090)
                     /gene="nth"
                     /locus_tag="ACHAY1_002848"
                     /pseudo
     CDS             complement(413..1090)
                     /gene="nth"
                     /locus_tag="ACHAY1_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1097..1795)
                     /locus_tag="ACHAY1_002849"
     CDS             complement(1097..1795)
                     /locus_tag="ACHAY1_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8594777.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2063..3247
                     /locus_tag="ACHAY1_002850"
     CDS             2063..3247
                     /locus_tag="ACHAY1_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8594778.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3253..3696)
                     /locus_tag="ACHAY1_002851"
     CDS             complement(3253..3696)
                     /locus_tag="ACHAY1_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8594779.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          980 a          753 c          628 g         1431 t
ORIGIN      
        1 ctcatgcaat gattctcact tttttatggt tgttgttcac cagaatctat atttttctga
       61 tgagcctgaa accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat
      121 tatcacttat catttttatt acacctttag atagatacgt cacttattta tataatcctt
      181 tggaatactg gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt
      241 ttcccaataa aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc
      301 tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc
      361 tccataataa caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc
      421 gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata
      481 ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatcaaccaa
      541 aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg
      601 tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac
      661 gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat
      721 ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt
      781 gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc
      841 gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg
      901 catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg
      961 tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt
     1021 ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt
     1081 tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt
     1141 cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt
     1201 cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt
     1261 caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa
     1321 tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag
     1381 aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt
     1441 gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga
     1501 tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga
     1561 taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca
     1621 tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca
     1681 actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta
     1741 aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt
     1801 ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt
     1861 tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc
     1921 atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc
     1981 cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat
     2041 tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg
     2101 atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt
     2161 tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat
     2221 gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca
     2281 ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct
     2341 ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac
     2401 ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag
     2461 aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat
     2521 tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt
     2581 tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa
     2641 acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt
     2701 caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg
     2761 ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt
     2821 ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat
     2881 gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga
     2941 aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt
     3001 atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca
     3061 actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat
     3121 acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc
     3181 atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc actacaataa
     3241 aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa
     3301 tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca
     3361 tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa
     3421 atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag
     3481 tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt
     3541 cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa
     3601 tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc
     3661 cagatattct tctattagca gttgtctttt atccatttct acaccttctt tttctttcat
     3721 tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat
     3781 ctgaaaccaa ga
//
LOCUS       DAYYCM010000139         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000139 DAYYCM010000000
VERSION     DAYYCM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.145"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHAY1_002852"
     CDS             complement(<1..353)
                     /locus_tag="ACHAY1_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594780.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHAY1_002853"
     CDS             complement(613..1161)
                     /locus_tag="ACHAY1_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594781.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHAY1_002854"
     CDS             complement(1162..2019)
                     /locus_tag="ACHAY1_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8594782.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHAY1_002855"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHAY1_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHAY1_002856"
     CDS             complement(2581..2910)
                     /locus_tag="ACHAY1_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8594783.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHAY1_002857"
     CDS             complement(2987..3259)
                     /locus_tag="ACHAY1_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8594784.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHAY1_002858"
     CDS             complement(3259..3522)
                     /locus_tag="ACHAY1_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8594785.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYCM010000140         3540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000140 DAYYCM010000000
VERSION     DAYYCM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.146"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHAY1_002859"
     CDS             complement(<1..2232)
                     /locus_tag="ACHAY1_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8594786.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHAY1_002860"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHAY1_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1003 a          779 c          551 g         1207 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
//
LOCUS       DAYYCM010000141         3531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000141 DAYYCM010000000
VERSION     DAYYCM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.147"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1030
                     /gene="galE"
                     /locus_tag="ACHAY1_002861"
     CDS             41..1030
                     /gene="galE"
                     /locus_tag="ACHAY1_002861"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8594787.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1042..2526
                     /gene="galT"
                     /locus_tag="ACHAY1_002862"
     CDS             1042..2526
                     /gene="galT"
                     /locus_tag="ACHAY1_002862"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8594788.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2550..3530
                     /locus_tag="ACHAY1_002863"
     CDS             2550..3530
                     /locus_tag="ACHAY1_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8594789.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1198 a          646 c          775 g          912 t
ORIGIN      
        1 atcaacaaac acaattttac taatcaaagg agtgtttttt atgacaattt tagtactagg
       61 cggagccggt tatatcggtt cgcacgctgt agaccagcta gttcaaaagg ggtatcaagt
      121 agctgtggta gataatttgc tcacaggtca caaacaagca gtacatccag atgctcattt
      181 ttatgaagga gacatccggg ataaagaatt tctacgttcc gtatttgaaa aggaaccgat
      241 tgaaggagta atccattttg ctgcaagctc tttagtagga gaatctgtcg aaaaaccatt
      301 gatgtatttc aacaacaacg tatacggtat gcaaatctta cttgaagtca tgcacgagtt
      361 caatgtgaat aaaatcgtgt tctcttctac tgcagcaact tacggagaac caaaagagtc
      421 tccaatcaca gaagatacac cagctaatcc taaaaatccg tatggtgaaa gtaaattgat
      481 gatggaaaaa atgatgaagt ggtgtgacca agcatatggc atgcgctatg tcgctttgcg
      541 ctacttcaat gtagccggag caaaagcaga tgcatctatc ggagaagatc atacaccaga
      601 aacgcatctt gtcccaatca ttttacaagt agctcttggt caaagaaaag cattggctgt
      661 ttatggagac gattatgata caccagatgg aacatgtatc agagattatg ttcaagtaga
      721 agatttgatt gctgcacata ttttagcttt ggaatacttg aaagaaggaa atgaaagcaa
      781 cttcttcaat ttaggcagca gcaaaggata ctcagtcaag gaaatgctag aagcagctag
      841 agaagtgaca ggaaaagaaa ttccagcaga aatagcacca agaagagctg gagatcctag
      901 ccgattagtc gcttcaagtg aaaaagcgag agaggttctg ggttggaaac ctgaatacac
      961 agatatcaaa gcaattatca aaacagcatg ggattggcat gtcagccatc caaatggtta
     1021 tgaagaatag gggaatgaga tatgtcaatc agtcaaacga tttgtgattt tgccacactt
     1081 gcgatcgaag ctggcggatg gatggaaatg gaccgcttat atttacagaa tcgtctttta
     1141 gggatggtag gagaagatgc tctagaaagc attgaagtaa gaccagttaa ggaagaatct
     1201 gtagacttat tggatcagct tgtcgctaag gcaagagaaa atggtgttct ttcagaattg
     1261 ccagcagaac aagagatttt tgaagcgcaa ttaatggatt ttctcacacc gccaccttca
     1321 gtcgtcaatg cattctttgc acagcactat tcgaaaagcc cagaggaagc aactgagtat
     1381 ttctttaatt taagtaagaa aaatgactac atcaaaacaa gagccatcgc aaaaaacatc
     1441 aaatttccgg cagagtcgga gtatggtgaa ctggaaatca caatcaattt atctaagcca
     1501 gaaaaagatc caaaacaaat cgcagcagaa cgcgaggcgg taaaagtaga ctatcctacc
     1561 tgcatgcttt gcatggaaaa cgaaggatat agaggtcgct tgaactatcc agcccgcacc
     1621 aatcaccgga ttattcgaat gaatctagac ggtgaaagct ggggattcca atattctcct
     1681 tatgcttatt acaatgaaca ttcgatcatt ttatcagaac aacaccgccc gatgaaaatc
     1741 acaaaggaaa catttgctag actgcttcgg attactgaag tcttgcctca ttactttgtt
     1801 ggttcgaatg ctgacttgcc gattgttgga ggatcgattc tttctcatga tcattatcag
     1861 gcaggacgtc atgaatttcc tatggcaaaa gcaccgatag aaaaacttat caaactagca
     1921 gattatccgc aagtcatcgc aggagtcgtg aaatggccga tgtctgtgat ccgtttgcaa
     1981 tcaacagaga aaaaattgtt gattgaagca gctaactcag tattggaaaa gtggaaagca
     2041 tattcggatg aaacagtatc tatcagagct ttttcagcag atggtacacc acaccatacg
     2101 attacgccaa ttgcacgaag aagggacaat tactttgagt tggaccttgt ccttcgagac
     2161 aacaatgttt cagaagaaca tcctgatggg atttttcatc cacataaaga tgtccaacat
     2221 atcaaaaagg agaacattgg gttgatcgaa gtaatggggc ttgctgtttt accacctcgt
     2281 ttgaaaccag aactagtaga agtagagcat ttcttattag gagaagaaaa ccagatggct
     2341 gaatatcatc gtccgtgggc agaacaatta aaaaaagaac atcctgattt gacgaaagat
     2401 acggtcaccg atgtcgtaca aaaatctgta ggtcaggttt ttgcacgtgt actggaagac
     2461 gctggagtat tcaagcgaga tgaaaaagga caggcagcgt ttgaacgatt tactgcaatt
     2521 ttataaacgt aaaaaaggag gagagacaaa tggcaacaat taaagacatt gcgcagttgg
     2581 caggagtttc tcctgctact gtttcaagag ttctcaacta tgatcctgat ctttccgttg
     2641 gcttagccac aaagcaaaaa atttttgaag cagcagaacg cttaaattat acgaaacatc
     2701 gtaaaaatgc agcaaatact aagcaaacgc ttcgcctgat ccagtggtac gacgatacgg
     2761 aagagcttga agatctttat tatttatcta ttcgtctagg aatcgaaaaa aaggcagaag
     2821 aagcaaacgt tactttattg aaagaaacgt gggattccat tagtgatctt ccgacagatg
     2881 gaacgatcgc actaggaaaa ttcgacgaag ctcagttatt agaaatgaaa ggatcccaaa
     2941 atgccttgct tttggtagat tctgacggca cttctcaagg aatcgactct ctagttgtcg
     3001 actttaagtc aagtgttcaa aaagtcattg aacattttgt ctcccataaa gcgcaacata
     3061 tcgctatgct tgctggaaca gaatatacga agaaaaacca tcagcggcta aaagatccca
     3121 gaattatggc atttcaacaa gttttggcag aaaaaggcta cgacgaaggt ccaattatcg
     3181 aagcagattt ttctgtcgag tcaggatatc aagcagtgaa gcagtattta aaaacgaatc
     3241 cgcatgttcc tgatgcgctt tttgcagcta atgatgcatt agcgattggc gcactgaaag
     3301 cgattcaaga aaaaggttta gtggtaccag aagatatttc tgtgatgggt ttcaatgatg
     3361 tcagtgtagc gaaatatgta acgccctctc tttctactgt aaaagtttat accgaatgga
     3421 tgggagagct agcagtggag acgattcttc aattaagtaa ttccaaagca ccggtttcaa
     3481 gaaagattat tgtggaaaca gagttgatta ttcgagaatc aacgaaataa a
//
LOCUS       DAYYCM010000142         3432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000142 DAYYCM010000000
VERSION     DAYYCM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.148"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1175
                     /locus_tag="ACHAY1_002864"
                     /pseudo
     CDS             34..1175
                     /locus_tag="ACHAY1_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1301..2380)
                     /locus_tag="ACHAY1_002865"
     CDS             complement(1301..2380)
                     /locus_tag="ACHAY1_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8594790.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2483..2923)
                     /locus_tag="ACHAY1_002866"
     CDS             complement(2483..2923)
                     /locus_tag="ACHAY1_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8594791.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2980..3390)
                     /locus_tag="ACHAY1_002867"
     CDS             complement(2980..3390)
                     /locus_tag="ACHAY1_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8594792.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         1267 a          656 c          598 g          911 t
ORIGIN      
        1 ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat cttaatatca
       61 acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac agaggagaca
      121 gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac ctttcaggat
      181 gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac agcgaccatc
      241 aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc tgttacattt
      301 gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac tatccaacac
      361 aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa accagcgctt
      421 tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc gtatagtcag
      481 cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt tgaagaacca
      541 acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga
      601 gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca accagagcca
      661 gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac tccagaacca
      721 gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact
      781 gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca
      841 gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag
      901 gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag
      961 ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc
     1021 aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt
     1081 cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt
     1141 ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca
     1201 tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat
     1261 atttggggtc gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt
     1321 ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg
     1381 gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa
     1441 atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag
     1501 actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc
     1561 cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga
     1621 tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag
     1681 ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata
     1741 ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag
     1801 aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa
     1861 aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta
     1921 aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa
     1981 tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat
     2041 taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga
     2101 atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag
     2161 ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa
     2221 atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata
     2281 ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat
     2341 cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa
     2401 taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca
     2461 taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca
     2521 tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact
     2581 tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg
     2641 taacatttac gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg
     2701 ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct
     2761 ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg
     2821 taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat
     2881 ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt
     2941 tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag
     3001 agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc
     3061 taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc
     3121 taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc
     3181 cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa
     3241 accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc
     3301 aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt
     3361 gatcattttc gtagaataca tttttttcat aaaattacca cctttaatta tattttatac
     3421 aattgaattt ta
//
LOCUS       DAYYCM010000143         3283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000143 DAYYCM010000000
VERSION     DAYYCM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.149"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1854
                     /gene="alsS"
                     /locus_tag="ACHAY1_002868"
     CDS             190..1854
                     /gene="alsS"
                     /locus_tag="ACHAY1_002868"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8594793.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1867..2577
                     /gene="budA"
                     /locus_tag="ACHAY1_002869"
     CDS             1867..2577
                     /gene="budA"
                     /locus_tag="ACHAY1_002869"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8594794.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2707..3204
                     /locus_tag="ACHAY1_002870"
     CDS             2707..3204
                     /locus_tag="ACHAY1_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8594795.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1111 a          537 c          722 g          913 t
ORIGIN      
        1 ctttcaatta agaatgacag ttttttatgt aaaaactgtc attctttttc attttttgaa
       61 acatatggct aaacaaatga aataaaacat gctatacttt tgaatgaaac aggtttagaa
      121 actgtatcac ttatttgtga ttatttctaa ctaataatta aaaataaagc ataaaaagga
      181 cgatgttcaa tggctgaaaa aatgaaacaa ggttcgaaat ctgtagtaga aagtttgatt
      241 aatcatcatg ttgattatgt attcggtata ccaggtgcaa aaatcgatgg tgtatttaat
      301 gaattagagg atcaaggtcc tgaattaatc gtgactcgtc acgaacaaaa tgctgcattt
      361 atggcacaag caatcggacg tattacagga gagccaggag tagttatcgc aacaagtggt
      421 cctggtgcta gtaacttagc tacaggtttg gtaacagcga cagcagaagg tgacccagtt
      481 ttagcaattg ccggacaagt aaaacgaagc gatctattga agttgaccca tcaaagcatg
      541 gataatgcag cactgttcca gccaatcaca aaatacagtg cagaaatcca agatccagaa
      601 acaatttcag aagtgattgc caatgcttat cgtatggcta agagttcaaa aaaaggcgcg
      661 agctttatca gtatcccgca agatgtagtg gatgcacctg tccaaggtaa tgtcatcaag
      721 ccattgagcg atcctaaatt aggttctgct tctgcagatg atatccgtta cctagctgaa
      781 cgcattagag aagcaaaact accggtacta ttagtaggta tgcgaggatc aagtgaaaaa
      841 gaaacgttag ccatcaggca attagttgaa aagacagcat tgcctgttgt agagacattc
      901 caagcagctg gagtgatctc acgtgaatta gaagcgcatt tctttggtcg tgtgggttta
      961 tttagaaacc aaccaggaga tatgcttttg aaacgaagtg atctagttat tgcaattggc
     1021 tatgatccaa tcgaatatga agcaagaaac tggaatgcgg aaaaagatgc gcggatcatt
     1081 gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc agcctgaaag agaattgatc
     1141 ggtgatatct cagccactct tgatttattg acaggttcat tagagcctca acaagtgtca
     1201 gaagatgcaa aagaatattt agcatctctt caagcaaaat taacggagcg ggacattgtt
     1261 caatcaaaag gagaagcagg tatcttgcat cctttagaag ttattaacac gcttcagtca
     1321 aaagtaacag atgatatgac tgttaccgta gatgtaggta gtcattatat ttggatggca
     1381 cgccacttta gaagttatga acctcgtcat ttgttattca gtaatggtat gcaaacgctt
     1441 ggtgttgcat tgccatgggc gatttctgct gcattggtgc gtccaaatac acagatcgta
     1501 tcagtttccg gcgatggggg atttttattc tctgcccagg atttagaaac ggcggtacgt
     1561 aaaaaattga atatcgttca tttgatttgg aatgatggac actacaatat ggtagagttc
     1621 caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact ttggtccagt tgattttgtg
     1681 aaatatgcag aagcttttgg cgctaaagga atccgagcta caagtgtaga ggaattagaa
     1741 aaagctttag aagaaggatt tgcgacagaa ggacccgtaa ttattgatat tccgattgat
     1801 taccgtgata atgagaaatt gggagaaaca atcttaccag atcaattcta ttaaggaagt
     1861 gtcgacatga ctgaaaaaat cttataccag cacgggacat taggagcttt gatggccggc
     1921 ttgatggatg gaacagaaac gattgctcat atattagaaa aaggaactct gggattggga
     1981 acattgcatg ggcttgatgg tgaagtcatc tttttagatg gcgtagctta tcaaggaaga
     2041 tcagatggct cagttgccca attagatgga agtgaactta ccccctatgc agcaattaca
     2101 gattttacgc cagatactag tttttctgtc tcaaaaactg cagatggaga acaattgaaa
     2161 aaagacatat tagtaagaga agccggggaa aacctatttt tggcagtgaa gataactgga
     2221 ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag aaaaacctta cagaagacta
     2281 gcaaaaattt cagagtctca accagaattc caacaaagca atgtccaagg aacattagtc
     2341 gggttcttta cgcctgaatt attccaagga gttgctgcag cgggatttca cctgcatttt
     2401 atcgatgata cacatacatt tggcggacat gtaatggatt ttgaagtggc tgaaggaaaa
     2461 gttgagatta gccgcatctc gtcccttgta cagcattttc cagttgatga tccgactttt
     2521 gttcacgaaa cgattgatta tgctgattta gcagcagaaa tccaacaagc agaatagaaa
     2581 aagacagcga gatgcgttca aggtgattaa cctgacgtat ctcgtttttt tgaggagaaa
     2641 acctagcgtt tacaaaaaaa ctgagctata atcttctcca tagatcaaaa aggagcggtt
     2701 tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg aaattttatt attacagcaa
     2761 caaggattat tagaagcaga agataactgg ttagcaaatt tagcaaattc atctgcattg
     2821 ttgaatgaga ctttgcctga gactgtcttt gcaggatatt atttattcga tggtaatgaa
     2881 ttgattttag gaccgttcca agggcgagtt tcttgcacac gtatcaagat gggaaaagga
     2941 gtttgtggag aatcagctga aaaacaagaa acattgatcg ttgatgatgt caaaacccat
     3001 gcaaattaca tttcctgcga ttcagccgca caatcagaaa tcgtagtgcc aatggtaaaa
     3061 gaaggtaatt taatcggtgt tttagatatc gattgcggag taacgaaagg gtatgatgaa
     3121 atcgatcaag catttctaga aaaatatgta gatcttttat tagcaaattt taatataaat
     3181 gctttttcat ttattaataa ctaaaatcgt ctattgaaac aaaaaagaag agaaatcaag
     3241 aataaaaaga gccggaaaga cagtgggcat ttctgttttt cgg
//
LOCUS       DAYYCM010000144         3279 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000144 DAYYCM010000000
VERSION     DAYYCM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.150"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..715
                     /locus_tag="ACHAY1_002871"
     CDS             <1..715
                     /locus_tag="ACHAY1_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8594796.1"
                     /translation="NIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVT
                     GIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNAL
                     KDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNL
                     VKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTE
                     LTRQMKRDAGAVGGTLYAT"
     gene            705..1445
                     /locus_tag="ACHAY1_002872"
     CDS             705..1445
                     /locus_tag="ACHAY1_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8594797.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1442..>3279
                     /locus_tag="ACHAY1_002873"
     CDS             1442..>3279
                     /locus_tag="ACHAY1_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8594798.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1235 a          397 c          631 g         1016 t
ORIGIN      
        1 taatattaaa cagtttttca aagatacttg gaaaaatatt aaagatacgg catacaatac
       61 gtggatttct ataaaaaata ccatgattaa tacatggaat aacattaagg actctttctg
      121 gaatattgtt actggaattg ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg
      181 tgtttcaaat gctattaatc gggtaaaaga aatctttaat tcgttaaggg aagtcaattt
      241 atttgaaatt ggcaagaaca tcattgatgg acttataaat ggtgtaaaag aaaagtggaa
      301 tgctttgaaa gatactatca aaggtattgc aggtaatatc aaaaattgga ttaaaggagc
      361 tttagatatt cattctccat caagatggat gcgtgatatg gttggtaaaa atattgttca
      421 aggtattatc gttggtattg ataaagagca aagtaagtta gatcaaacca tgacaaattt
      481 agtaaaaaca ccgtctgttc aacctgtagt aaatggaata agtcacctac caattacaca
      541 aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt catttacatt taaatgttta
      601 tggagattta cctgattcaa tggttcgaaa gattactaca aaaattaaaa cagaattgac
      661 aagacaaatg aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa cttaagagag
      721 gacaattttt tattaatcaa cattattctt ctgaatttaa tgtgtatatt caaaacaggc
      781 ctgcttctgt ttcagctagt cgagtaattg aattgagaga gcgagaagga aatgattcta
      841 tcattattga taaaggctat tacaaaaatg tgactagaaa gattgagtgt tactataaag
      901 caccttcaat tgatatggtg caggagtggg aagatcgaat cactgaatgg ttagatatgt
      961 cgtcttatag tgattttatt ctttactatg atgaacaata tatttatcaa gctgtagtaa
     1021 tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct tttgaattta
     1081 cagtgagtat tcgaccattt aaggaaaatt acaatggtag atttactatt cgacaaacag
     1141 aaacttttga aatttataac ccagagaagt attcttcaaa accgcttatt aaattgagtg
     1201 gttctggaga tgcttctttt tatattaata aggataaata tgatttgaaa tcgttggaca
     1261 gagaattatt tatagattct aaattagaag aggcttatcg aaaattagat ggtaatttag
     1321 aacatcagga tcaggtcacc ttatttttag attttccatt tttatatcct ggaaaaaatg
     1381 aaattaaatg gacgaaaaat attcattcat ttgaaataat gcctaggtgg tggagaaaag
     1441 tatgaaacca agaatatata gtcctactga aacagatttc tcaacgaatg gcttaggaat
     1501 tttaaaggat tgtacaagat gtgaaatata tgaagtagct aatggaaaat atgaattaga
     1561 gttggattat cctttaggaa ctagatttga tgaatatttt gaaaatgact atcaaataaa
     1621 agcaaagcca aacgatcaag aagagtatca tatctttttt attgatgata aagatataga
     1681 tactttttta aatacagtaa ctatttatgc tcagagtcgt acaaatcgac ttggaagacg
     1741 ggtagtcact cttgtggaag tggactctaa aactggtcaa gaagcgatat caattattga
     1801 aactaaaatg gataaaaaat ctgacatacg actttattct gatattacgg ctgtttctag
     1861 cacaatcttt gaagcgagaa atgttttaaa ttgtattgct ggtgaacaag gatcattgct
     1921 tcaatattgg ggcggagaaa ttaaacgtga accatttaag ctttctttgt taaagcgaag
     1981 aggtcgcaat aatgttggaa cgattcggta tggaaaagat atgtctggtt tgaaggtcaa
     2041 attagattgg acaggtataa aaacaagaat tattccatat gctgatcctc aaagtgaagt
     2101 aggtacgact aatcgaattt atggttcgcc agtagatagt gcctatatta ataactatcc
     2161 tgatgtctat acagaacatg ttcagtttac agaagaacaa ggagtaaaag acattaagag
     2221 cttaaataaa atagctaaaa attactttaa aacgatcaat cctggctgtg ataagcctaa
     2281 agtttctatt acggttgaat ttgataagtt gactgatagt gaagaagcga aagaatttgc
     2341 gaagattaga aattatggtc tgtttgatac atttaaaata tatcataaaa aatataatat
     2401 ttatcttgaa tcaaaggtta gtggactaca atacgattct ttatctgaaa aaactttgaa
     2461 attagaagca ggagacattc aagttgcttt ttatcagcaa caagctgtaa ctattcagga
     2521 taaattaaaa gattatgcaa cgaataatta tatgagtgat tttaatgatt atgtttcttc
     2581 aatgattaca ggtcaaggca gtgcaggagg atatgtagtt ttatggccaa aagaaaaacc
     2641 ttccaacatc tttattatgg ataatccaga tttagaaaag gcaagagaag tactaagaat
     2701 gaataaaaat gggattgctt tttctaagaa aggatggcgg gggcctttta attcagcttg
     2761 gacattagat agtatattta atgccaattt tatacaaact ggattaatca aagcagatat
     2821 ctttcaaaat tcttttaaca agactggaga tgtattgaaa ttagttaatg gactactcca
     2881 aatttggaac aacaaaaaga aaataatgga gttaactaaa aaaggaatgg aattttggaa
     2941 ttctaagggt tcaattggaa cgattggaac aactgattct gttggtaatc cttttcctgg
     3001 ggcttctact ccaaccccta ttgaagataa ttctttagtt attcgtacaa atggagacgg
     3061 caaatatatt ttgatttctc ctaaagtagg aaaaggatta gttttattag gaaatggtaa
     3121 agcaatttat tttggagact tagatgtaca aggaaaactt acagttaaag gaaaagaaat
     3181 aattcctgga caaggtggtg gtccatcggg aggtggagaa tctactggtg ggtatcctag
     3241 tgaattaaaa acagatgcag aaaagagagc ttggagaat
//
LOCUS       DAYYCM010000145         3261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000145 DAYYCM010000000
VERSION     DAYYCM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.151"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..212
                     /locus_tag="ACHAY1_002874"
     CDS             <1..212
                     /locus_tag="ACHAY1_002874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594799.1"
                     /translation="INLPNKFIGGFKMSHTLLIKDTLNILDLNINIHFEENCLTKEKY
                     KGQICLIYKGTLQYTPEEYPHLPIY"
     gene            272..505
                     /locus_tag="ACHAY1_002875"
                     /pseudo
     CDS             272..505
                     /locus_tag="ACHAY1_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(553..672)
                     /locus_tag="ACHAY1_002876"
                     /pseudo
     CDS             complement(553..672)
                     /locus_tag="ACHAY1_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(1888..3054)
                     /locus_tag="ACHAY1_002877"
     CDS             complement(1888..3054)
                     /locus_tag="ACHAY1_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8594800.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1096 a          623 c          439 g         1103 t
ORIGIN      
        1 gaattaattt gcccaacaaa ttcataggag gattcaagat gtctcatact ctgcttatca
       61 aagataccct aaatattcta gacctaaata tcaatatcca tttcgaggaa aattgtttaa
      121 ctaaagaaaa atacaaagga caaatttgct tgatttacaa agggactctt cagtacacac
      181 cagaagaata tccacatctt ccaatatatt aaaagaacct tcttgaatca ttgagtgaaa
      241 gaaaagagat tcgtcactat ttgaggactg tttaactaga gacattagcg atctgcctga
      301 aacctataca accactttac gaacgtttaa aaattttctg ccacagattc aaaatgcgct
      361 taactgcagc tattcaaaca gaccattgga atgtttaaac aaccacatca aagtattaaa
      421 acgcaatacg tatggctttc gcagctttta caatttcaaa ctacggatca tgattcgtca
      481 tggcaaaaca tttttaacca aataaacaga agaatgtggg ataaatctaa aaatcagttt
      541 tgctacagac tctcgagtaa acggcgggag cagaagcaac tccttcggaa ataagccgaa
      601 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttctcg gagttaaaca
      661 cttctgtccc agcctcttct gtccaaatta ttctaagatt tctctttacc aacatcattt
      721 gacaaagagc ttcttttttg aatactatat tcgttctgtt tttcatcttc aaatacgaac
      781 tcatccgcag aaatatttgt cacaactcca tgaatcttct tttcattacc acctatatac
      841 cacaggattc ctacacagaa acaacaaaaa aatagcataa aataggtttt ctttatttta
      901 tatccctcct tttagattaa aagctcacag aaaacgcttt ctaaaaagac aatagaataa
      961 tcataatttt tcattttgta ttatttatac aaataatacg catgttactg aaaaatgata
     1021 tggttcccta ctttttgaga taagttaaat aaaatctgtc atcgataaaa cagctagtac
     1081 agtaatgata aatagtaaga acttaaaata ttttggattt ttatttttga aataatcgaa
     1141 gaaaagtaaa agtgataacg aaatagcaaa caagattata tctataagat tattcattga
     1201 gaaatcgccc catatggaac tctattttga cctgacacac cgagacataa agcaatcata
     1261 tcctctgcat tgttttatac tattagaact atagctacac ctattggatg ctactggctc
     1321 taagattaat gctaatacag taacatcaat acctaaacta gcagaaactc tatttgcatc
     1381 ttcaatttca gatgcctgga ctgtcgtgac tagcgccgta gtagaaaaat ttactggtac
     1441 taacaaagat gcagacacga cttttatttc acttatcctt tatcccataa cctaaacttc
     1501 aatcattact tgtgagctga tcaatgttct tccaagtctg aaagaacatt gatcagcttg
     1561 gcaaacaaat aatgttttca aatacttgat cgatgaaaaa aagctattgg taataacagt
     1621 aattgcattt tccattttcg tgtcagatac ttcgcttaca gctggtaaac gatcaatccc
     1681 attgtcaaat tttggatttt tttctgatga tgtcttgctt tagttcataa aataatccag
     1741 caataaattc ctcgcaagga tagccaactt cattttccta atcggaattt ttaaacttat
     1801 cggtatagat acagatgcaa tgagaacaaa catttatttc gagtcaccat ctaaaatatt
     1861 ttcctccatg tacccgttca ttattattta gttatcacca aagatatctc tggtataaac
     1921 tttttcttca acatccttta aaatatcttc aaaccgatta gatacgatca catcagaaat
     1981 atttttaaat tcattaatat ctttcaatac ttgtgaatga taaaattcat cttcatccaa
     2041 tgtaggctcg taaacaacca cttcaattcc tttggcttta atccgtttca taattccttg
     2101 gatcgacgaa tatcgaaaat tatctgaacc ggccttcatc gttaaacgat agattccaac
     2161 aacatttggt tttttcgcca aaatctgtcc agcaataaaa tctttccttg ttgtatttgc
     2221 atcaacaatt gcactgatga tattgcttgg tactttttca tagttggctc ttaattgttt
     2281 tgtgtctttg ggtaaacagt atccaccata gccaaaagaa ggattattat aatgcgaacc
     2341 gatccgtgga tccaaaccga caccttcaat aatttgctta ctatcaagcc cacgaaattc
     2401 tgcatacgta tctaattcat tgaaatacgc cactcgcaat gccaaatatg tattcgagaa
     2461 taatttaata gcttctgctt cagttgagtg agtcaaaaga actgatgcat ctttgttaaa
     2521 tgcgtttctt tgaaacatag ttgcaatctc gcgaccacga gcagaacatt ctccaactat
     2581 aattcttgat ggatataagt tgtcgtacaa agcttttcct tcacgcaaaa attcaggtga
     2641 aaaaatgatg ttttctgtac caaatttcgc tttaatttca tttgcatagc caactggaac
     2701 tgtcgactta ataatgaatg ttgaatctgg acgaattgct aaagccttct caattacatc
     2761 ttctactgtt gatgtattaa aatagttttt cttttcgtca taatctgttg gagttgcaat
     2821 aatcaagtaa tcaccgaagt ttactgcatc agtaaaattt gacgtaaatt cgacatttaa
     2881 gtcatcccgt tttaaaaatt catgaacttc tttatcttct attggtgaga tgccattttg
     2941 taacatcgtg attttttctt ccacgatatc ataagctttg acctgctctt tttgtgctaa
     3001 taatagtgaa tttgctagac caacatagcc taatccgaaa actgaaattt tcatattctt
     3061 ccatacgctc caatcaaatt tcattttata gtattttagc tgttatcctt ttgaacatat
     3121 tccttattca atcaatatta tttaatagaa aaaatttttt tgcttcaaaa tcatcgaggt
     3181 tcgatattta ataaattcat ttttattttt ctcataatgc tcctctatag ttgaaatcac
     3241 tttacatgaa ctacccgcta c
//
LOCUS       DAYYCM010000146         3213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000146 DAYYCM010000000
VERSION     DAYYCM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.152"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..152
                     /locus_tag="ACHAY1_002878"
     CDS             69..152
                     /locus_tag="ACHAY1_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8594801.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHAY1_002879"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHAY1_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8594802.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="ACHAY1_002880"
     CDS             complement(1277..2140)
                     /locus_tag="ACHAY1_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8594803.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="ACHAY1_002881"
     CDS             complement(2142..2414)
                     /locus_tag="ACHAY1_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8594804.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="ACHAY1_002882"
     CDS             complement(2431..2640)
                     /locus_tag="ACHAY1_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8594805.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2739..>3213)
                     /locus_tag="ACHAY1_002883"
     CDS             complement(2739..>3213)
                     /locus_tag="ACHAY1_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8594806.1"
                     /translation="RLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAIVASDYVMIPLQ
                     AEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSNLEELYKEHKED
                     NLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERIIQLENEKE"
BASE COUNT          978 a          566 c          509 g         1160 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattaat cacttgtgat ttgtgattct attctttttc attttctaat
     2761 tgaatgattc gctcaatcat ttcaaaaaat acgttctcat acatagacaa aacttttttg
     2821 tcatagcctt tgtgttctgt aatgccattt ttagaccaag tacttacttt attacttcgc
     2881 ttgataatat ttcggaaaac caaattatcc tctttatgtt ctttgtacag ttcttccagg
     2941 tttgatttta tcgttgcgct gtccgtatca actaaataag gaacaaaacc aatcatatct
     3001 agtccagggt taaactgttc ttgcaaatca atcaaatagg aaatatagtt ttgaatgttg
     3061 tttgtacttt cttcttctgc ttgtaaaggg atcataacgt aatcactcgc cacgattgca
     3121 ttatttgtat aaacgcttgg cgttggtaca gtatcaataa taataagatc atagtcactt
     3181 tttaaaggtg ctaaaagagt agcaagcaat cta
//
LOCUS       DAYYCM010000147         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000147 DAYYCM010000000
VERSION     DAYYCM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.153"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHAY1_002884"
     CDS             complement(63..2555)
                     /locus_tag="ACHAY1_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8594807.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHAY1_002885"
     CDS             complement(2562..3029)
                     /locus_tag="ACHAY1_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8594808.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAYYCM010000148         3059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000148 DAYYCM010000000
VERSION     DAYYCM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.154"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..951
                     /locus_tag="ACHAY1_002886"
     CDS             184..951
                     /locus_tag="ACHAY1_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594809.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            941..2983
                     /locus_tag="ACHAY1_002887"
     CDS             941..2983
                     /locus_tag="ACHAY1_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594810.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          988 a          488 c          617 g          966 t
ORIGIN      
        1 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
       61 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
      121 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
      181 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
      241 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
      301 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
      361 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
      421 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
      481 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
      541 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
      601 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
      661 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
      721 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
      781 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
      841 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
      901 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
      961 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
     1021 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
     1081 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
     1141 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
     1201 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
     1261 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
     1321 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
     1381 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
     1441 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
     1501 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
     1561 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
     1621 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
     1681 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
     1741 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
     1801 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
     1861 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
     1921 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
     1981 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
     2041 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
     2101 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
     2161 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
     2221 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
     2281 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
     2341 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
     2401 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
     2461 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
     2521 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
     2581 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
     2641 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
     2701 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
     2761 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
     2821 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
     2881 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
     2941 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
     3001 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacg
//
LOCUS       DAYYCM010000149         2861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000149 DAYYCM010000000
VERSION     DAYYCM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.155"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHAY1_002888"
     CDS             <1..1087
                     /locus_tag="ACHAY1_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594811.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHAY1_002889"
     CDS             complement(1305..2447)
                     /locus_tag="ACHAY1_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8594812.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHAY1_002890"
     CDS             complement(2452..2637)
                     /locus_tag="ACHAY1_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8594813.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          949 a          526 c          448 g          938 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa g
//
LOCUS       DAYYCM010000150         2755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000150 DAYYCM010000000
VERSION     DAYYCM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.157"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHAY1_002891"
     CDS             complement(139..1338)
                     /locus_tag="ACHAY1_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8594814.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..>2755)
                     /locus_tag="ACHAY1_002892"
     CDS             complement(1468..>2755)
                     /locus_tag="ACHAY1_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594815.1"
                     /translation="LISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNV
                     KGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTG
                     VNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDA
                     DNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQ
                     DATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFG
                     SLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYF
                     LAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKF
                     MIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          842 a          531 c          412 g          970 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgaga
//
LOCUS       DAYYCM010000151         2701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000151 DAYYCM010000000
VERSION     DAYYCM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.158"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..398
                     /locus_tag="ACHAY1_002893"
     CDS             75..398
                     /locus_tag="ACHAY1_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594816.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            562..816
                     /locus_tag="ACHAY1_002894"
     CDS             562..816
                     /locus_tag="ACHAY1_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594817.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            886..1131
                     /locus_tag="ACHAY1_002895"
     CDS             886..1131
                     /locus_tag="ACHAY1_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594818.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1207..1572
                     /locus_tag="ACHAY1_002896"
     CDS             1207..1572
                     /locus_tag="ACHAY1_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8594819.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1633..2196)
                     /locus_tag="ACHAY1_002897"
     CDS             complement(1633..2196)
                     /locus_tag="ACHAY1_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594820.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          791 a          477 c          423 g         1010 t
ORIGIN      
        1 tgtcataact attttgttac tttaattttt atttgcttat tttagaaaaa atgttattct
       61 tttatttatt ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt
      121 tagttgcggc agcagcttat tttctccaat taaattatca aacctcagga attctgttta
      181 ttggaatgat ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata
      241 aaggaaaaag atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct
      301 atgggatcat cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga
      361 attacttaga tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca
      421 gttcctgctt atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct
      481 tttattttcc tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg
      541 aatcgttgag gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg
      601 gtcgcactgg tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta
      661 gctattttga ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt
      721 gaacccgata gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc
      781 attctttatt tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct
      841 ttctatagtc attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa
      901 gctattttac tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta
      961 tctcaagtag ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa
     1021 gaaggagaag aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt
     1081 tccgttgatt acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca
     1141 gccttggtgc tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac
     1201 atttctatgc gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta
     1261 ggacaacatc gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact
     1321 gtggattatg tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg
     1381 gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt
     1441 ggcaaatcat gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat
     1501 ccggaacacg atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt
     1561 tatcttacct aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt
     1621 aaccatctta gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc
     1681 aaaactaaaa gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca
     1741 tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag
     1801 attgcttctt ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt
     1861 actacatcga tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc
     1921 tctgtagtca ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt
     1981 tgtgatcagt tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat
     2041 tgatagtaaa aatagttttt ttatcataag gttccccccc tgaaaataga ctaaaataat
     2101 aacgctttct ttttttgttt tcagacgaaa attaataatt tctaaatatt ctttaaaaaa
     2161 gcttataact attatgcctt ttttaatata taacacagtt atttttaaaa ataagaatgc
     2221 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
     2281 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
     2341 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
     2401 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
     2461 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
     2521 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
     2581 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
     2641 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
     2701 t
//
LOCUS       DAYYCM010000152         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000152 DAYYCM010000000
VERSION     DAYYCM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.159"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHAY1_002898"
     CDS             699..785
                     /locus_tag="ACHAY1_002898"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8594821.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHAY1_002899"
     CDS             complement(1087..1311)
                     /locus_tag="ACHAY1_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594822.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHAY1_002900"
     CDS             1677..1961
                     /locus_tag="ACHAY1_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594823.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHAY1_002901"
     CDS             1964..2380
                     /locus_tag="ACHAY1_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACHAY1_002902"
     CDS             complement(2517..>2675)
                     /locus_tag="ACHAY1_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8594825.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAYYCM010000153         2431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000153 DAYYCM010000000
VERSION     DAYYCM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.160"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..2258
                     /locus_tag="ACHAY1_002903"
     CDS             72..2258
                     /locus_tag="ACHAY1_002903"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8594826.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT          931 a          410 c          482 g          608 t
ORIGIN      
        1 caacgaaact ttaaataacc aacttgaaat aaacaaattc aaagaagaaa gatgggaagg
       61 aaatcaatca gttgaatgaa aaagaaattc tccgattaca aaaagaattg aaacgctggg
      121 agaacaagta taaaaagctt caacaagtct ttcggctaca agaagaaatc attgctacct
      181 atgaagcggc ttatggcgaa gcacccggac ttattgagga agaagtacaa gtaaaagaaa
      241 gtgaaggaaa cagctctttc tttaaaatga tggagaaacg ttcctataat cgcttcaatc
      301 aagaacaaaa agcagctatc acatatgata tgaagaaaca tcttcgaatc atagcaggag
      361 ctgggagcgg caaaacgcaa acaatctgtg caaaagctgt ttatctgatg acgcaaaaac
      421 aagtagacga agaacgcata ctaatgatca cttttacaag gaacgctgca aatgaactaa
      481 aaaaacgagt agataatttc agccagagaa aaaccgctgt ccatatcgga acattccata
      541 gtatcttttt taggatatac aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag
      601 gaatacaagg agacttttca aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc
      661 tgatacgtaa atataatctt tatatatttg atcaatatgg agaaaaaacg attgcttctc
      721 ggttagacta ttggcagaat atgaaccttt cagtagaaga aatggttcag ttagttaaag
      781 aaaaatatga ttccattgat aagaatgcac gacagccgat atcagaacga ctgtatggat
      841 tgctcacgga actccaagaa caaaaaagaa gccagcaatt gctagagttc aatgatgtac
      901 tccaaaatct gaaaagcgct ttggagaatg aggagatcca gcattatatc ggccaaaaat
      961 atgattatct attcattgat gaatttcaag atactaatcc acttcaatgg gaaatcgtga
     1021 aacggatgac aaaggaaaac aggatcaagt tgattgttgt tggagatgat gatcagagta
     1081 tctatggatt cagaggatca gagccagcat acatcaagaa ctttgaaaaa gaatatccta
     1141 ccaaaactct tttcttatta actaattatc gttctcgggc agctattgtc caaggagcaa
     1201 atagattgat cagctataat aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa
     1261 aagaagcagg tgttatggaa gcctatttgt tttctgatac taaagcagaa agtcagtggc
     1321 taatcaaaca aattcagaca tttattcaaa aaaatgggaa atataaggaa tcaatcatat
     1381 tataccgatc agcttcacaa acgcagcagc ttgtccaatc tctgctgaaa acgaatattc
     1441 cttttgtact ggaagcagat agtccatatg aaggtatctt tggtatcaag tctttccaac
     1501 atttctatca aaaaattgtt cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg
     1561 cctaccagca attgctccgg cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg
     1621 atcaatactt ttctcctaaa tatcaaaata tagccgttgc agattatatt ttgtcgataa
     1681 gccccaattt aaaatcgaaa tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa
     1741 cagaccagca tcaagaaatc tattcgcttg ttcaaagcta tcttcatctg ccacgtatag
     1801 ctaaggaaat cgaccagagt gatcaagaat ggatacgaga agaagtaaag cagcatgcta
     1861 ctttcagaag actccaagcc ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa
     1921 cccgcttgat tgcttacaga caaggaaaac ttgatgcact atgtatccag agcattcaca
     1981 aaagtaaagg tcttagttac cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc
     2041 cttataacgg agcgacagaa aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag
     2101 taacgacgat agaagaagaa cgaagactat tttatgttgc gatgactaga gcaagaaatc
     2161 gtctgtatct atgtgtacct caaatgaaaa attcaagaaa attgaaagta tcgagattta
     2221 tcaaagaaac cgggagtcgt gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa
     2281 gtggtcagtt tcaagaaaga agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa
     2341 atttcagctt attttcgaag aagctatttc tggaacacag tttattcgga aaagggaggt
     2401 gtgacaagaa ttttgtcaca cctcccttat t
//
LOCUS       DAYYCM010000154         2428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000154 DAYYCM010000000
VERSION     DAYYCM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.161"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHAY1_002904"
     CDS             316..570
                     /locus_tag="ACHAY1_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8594827.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHAY1_002905"
     CDS             571..888
                     /locus_tag="ACHAY1_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8594828.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHAY1_002906"
     CDS             1447..2382
                     /locus_tag="ACHAY1_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594829.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          878 a          343 c          457 g          750 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaa
//
LOCUS       DAYYCM010000155         2426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000155 DAYYCM010000000
VERSION     DAYYCM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.162"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2276
                     /locus_tag="ACHAY1_002907"
     CDS             123..2276
                     /locus_tag="ACHAY1_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8594830.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          829 a          426 c          536 g          635 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAYYCM010000156         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000156 DAYYCM010000000
VERSION     DAYYCM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.163"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAY1_002908"
     CDS             207..2069
                     /locus_tag="ACHAY1_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8594831.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHAY1_002909"
     CDS             2069..>2400
                     /locus_tag="ACHAY1_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8594832.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYCM010000157         2212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000157 DAYYCM010000000
VERSION     DAYYCM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.164"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1015)
                     /locus_tag="ACHAY1_002910"
     CDS             complement(<1..1015)
                     /locus_tag="ACHAY1_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8594833.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFL"
     gene            complement(1079..1438)
                     /locus_tag="ACHAY1_002911"
                     /pseudo
     CDS             complement(1079..1438)
                     /locus_tag="ACHAY1_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1616..1936)
                     /locus_tag="ACHAY1_002912"
                     /pseudo
     CDS             complement(1616..1936)
                     /locus_tag="ACHAY1_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          677 a          424 c          310 g          801 t
ORIGIN      
        1 ctaagaaata aaaggcattg gcacaggtca attgaatgat atttttcaaa ttgccaacat
       61 tttcactaaa gcgatagttt aataaaaaat ctaataattg ttgagatatc agtattttct
      121 ttgctagtct ttcttcttct gatttaaaaa atatcttgat taactgtttt ctttcataaa
      181 gagaacgact gtctaatgtt ggaagtgtta gctttacagg tattcttctc aacaaagttg
      241 ataatagttt atcctcaggt ggttctgatg ttgcaaatat aatccagcaa cttgatgtgt
      301 accagttttc attatcaccc atccgatgaa atttcctcgt atccataaac tgaaaaagtt
      361 tctcctgaca ttcattgctc aatgcatgaa tttcatctaa aaataagact ccattattag
      421 ctaattctgc taaacccttt cttccatctt cggctcctgt atatgcacct tttacatgcc
      481 caaaaaaatt ggacaagaaa agttctggat tattggcata ttcactacaa ttaactgaaa
      541 tgaaagatgc cttctcttct atataattat tggccaacaa atccttatac acataatttg
      601 ctaaatagct ttttccagtt ccagtctcgc catgaagtaa aatgggtaag ccatttggcg
      661 gatagtagat tgctgcttta atttgagcaa ttacatttcg cagacttcct tgacttccga
      721 taaccctttc tattccaacc ctaaaagagc tatcatacca tttttccgct tcctctgaag
      781 agctaaaaat ttgttgattt ggaactttct ccggtaaaga aaagaagatt actggacgag
      841 tattgatttt tccaaattct ccatctttta cgaattcatt caaatattga gagacaatat
      901 tacgactaac atttaatttt tcagatattt tctgtgctgt attgttcccc catttatttt
      961 taatctcaga gttctctatg acataattga caatgcgttc ttttaattct tccatcacag
     1021 cacctcctcg acactattcg aattttgccg acagtatttt tccataaata tgacttgcta
     1081 aaaaggcttt gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga
     1141 cgttgaaaag atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca
     1201 gatcttcttt tgattcttca tctgaaagta attcacgata caacatcgtt tgtttgctta
     1261 ctgtgtcata ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt
     1321 cggataaata tttatagaaa atcagcccca ataaataatt tttatattct gacgcatcca
     1381 ttttactacg gagattatct gccgcactaa ataacttctg atttaattct gcacccaaaa
     1441 agatacactc ctatctctta tttaaagttg ctatataccc tcttatttta gcatactcag
     1501 ttttagaaaa agcaaaaaaa taaagaaact tattcttgat aacaagctac gtgacgtata
     1561 tccctaacac aaaaatagcc atatcctttg gtttggatac ggctatttga tattactcat
     1621 tcaattatca aatcacagta ggtgtgaatc tcatcgggat cggcaatatc tacttctgat
     1681 taggatcaaa ggtgattttc tcaataaagg tgtgatacaa agctttaact tgcttcttct
     1741 cactctggat aagaaagcaa tccaaactag tcaatatctg ttggacatct ttgacttgaa
     1801 tcggttcccc ttgttgttga aagatcccta agatttcatt ctctcgaatt tgtaactgtc
     1861 ttcgtttttc agtcaattca tttaaacgat cttttaacat cggaaacaat tcgggactat
     1921 cttccaaagc agcttcctgt ttttgctcta atagttagat tttgcgtacc attttcttct
     1981 tttattttcc ttcgattgct gattttaata tagtctaaag gaaatttata tttattgtat
     2041 tctttataac ttcaagagaa cttcgtttga gtattactgt atttttttaa ttccataaga
     2101 aaattagtta ttacctgcca cctaatcagc aaatctgaac ctgagtcaat ataattaata
     2161 ttattttcca tctccaacat tgtaagtact gttggtgtta atatagggac tt
//
LOCUS       DAYYCM010000158         2194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000158 DAYYCM010000000
VERSION     DAYYCM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.165"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..575)
                     /locus_tag="ACHAY1_002913"
     CDS             complement(<1..575)
                     /locus_tag="ACHAY1_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8594834.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRM"
     gene            complement(1158..1340)
                     /locus_tag="ACHAY1_002914"
                     /pseudo
     CDS             complement(1158..1340)
                     /locus_tag="ACHAY1_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1799..>2194)
                     /locus_tag="ACHAY1_002915"
     CDS             complement(1799..>2194)
                     /locus_tag="ACHAY1_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8594835.1"
                     /translation="MYNKKLEQETKRKIVPPEIKTWWRLELQLRRGKATDWYAMVHES
                     LDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYKYRNLLKKESQSDELVN
                     HLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          631 a          507 c          346 g          710 t
ORIGIN      
        1 tacatcctta tttgtcgctc actggcacga ctaccccaat atgccgtttc cagcttccca
       61 gaccgcccat agattggttt gaatgaaaca gggtcaacca cacgatattg cgtaataaaa
      121 tcatcaggca catcgataat gtcgcaagca atatcagcac gtgagaaatg tggttctaaa
      181 aataaatcat ggataaagtt cttcatcgaa cccgccaaaa attggctaat tttatttggg
      241 ttaaagtcaa ttcgcccttg cccttcttga tatttcaaaa tttcaatgta agcaatattc
      301 tcatcatgct tatccacaat ttgccaacca tttagaccaa cagatttaac ataaccatta
      361 ttttcattca gtcgcattaa ctgttcaaag tctaaaatca atacatcatt ttgcgtgtga
      421 taataaatat tctctttgag ttttccaaca atggtaattc ggtcaattga ccactgtatt
      481 ttaagggggg tggtcaaagt ttgcacactt aaccgcctgt tagatagggc tgttaaattt
      541 tcccactcat ttatgatcgt gccacgttta ctcaaatttt gttcctcctc aaagaaaaag
      601 agacttttgg aaagaatttc cagagttcac gtaaccctaa aaattcatat ccaaaaatct
      661 ctagcttgaa gaaacctaaa aatattcgta taatgaacct gagttacgga gctaggtgaa
      721 gtagtttccg taaatagtcg agagtgttag cgcactgctc ggcttttatt ttgctctttt
      781 ttctcgtttt ctttgattat agcactcctc ctaaaaaaat aaaaagacct cagggaattt
      841 cattccccga accccttgag cctcctcctg ccgaaatgga aagggcgcac ccgctgttta
      901 tttcattcag ctatcggctt tcacaatcta acacgatcgt tccgctacca gtcgccttac
      961 aggctccatt ttcgctcctc taacgtgttt taacgattgt gccgataact aaacgaaata
     1021 aacgctaaaa ccgctcagaa cgcaaaatat gccattttag agctattgta gaaaatgtag
     1081 tttgaaatgc aattttcttc tttcagcttt ttatttccca cttgtaaatc tgctaattat
     1141 tttctaccag acaaaccatt gattttttgg ctatcagtta actcacgatt attatgagaa
     1201 atgcttgtat tctttgttcc ctttttgaac gaaatactaa accctaaggt gaacccctcc
     1261 ttcgcgctgt cgctaataaa aagagtgtaa acctttctgt tactctttga caaagagtaa
     1321 cattttccta attgtgacat ggctgtcaca attccaaacg ctctccgagg gttttcgctg
     1381 tcacaagtga caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc
     1441 tttattttgc ttcacgcaaa ataatcgctt atcttaaaca ttgacgtaaa tttactctca
     1501 cttatctgac ttttttactc ggtatcactc cgagtaagcc gtcatattca ttgggataat
     1561 ctcgtctgct tcgctctaat tttccagagt gaggggtaac tccttccccc gaattacccc
     1621 aaagagtgac actgggatat ccctaaattt attataaaaa gccaagtctc tttgcgcaac
     1681 tgttttatcc aagtcggcat ttgcggattc gttagcgttt tttcgctctt gctgcaaacg
     1741 cacgacccac catgccgact tgtgctgaaa ataaaaaagt ggacaaaaat tcatccactc
     1801 attcttccgt cacgtccaac cccaaaagcc acgtatccaa ttcttttttc aaaacatctg
     1861 ccgattccga aaatgtctct cgtaaatgat taactaactc atcactttgg ctttcttttt
     1921 tcaacaaatt tcgatattta tattttgtat gacgagccaa gccagcccat aaattttgat
     1981 ctgaaatcaa tcctgcaatc ataattctat cagtatgctt tgtatcagtc ggcaaaaaat
     2041 gaggacttgc gaaactgtct aaactttcat gaaccatcgc ataccaatca gtagcttttc
     2101 cacgccgtaa ctgcaattcc agtcgccacc aagtttttat ttccggtggt acaattttcc
     2161 gtttggtttc ttgttccaat tttttgttgt acat
//
LOCUS       DAYYCM010000159         2164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000159 DAYYCM010000000
VERSION     DAYYCM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.166"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHAY1_002916"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHAY1_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHAY1_002917"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHAY1_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHAY1_002918"
     CDS             1898..2059
                     /locus_tag="ACHAY1_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594836.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          783 a          335 c          366 g          680 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agtt
//
LOCUS       DAYYCM010000160         2159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000160 DAYYCM010000000
VERSION     DAYYCM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.167"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAY1_002919"
     CDS             <1..156
                     /locus_tag="ACHAY1_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8594837.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHAY1_002920"
     CDS             175..1452
                     /locus_tag="ACHAY1_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8594838.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHAY1_002921"
     CDS             1462..2118
                     /locus_tag="ACHAY1_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8594839.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          907 a          191 c          369 g          692 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagttta
//
LOCUS       DAYYCM010000161         2088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000161 DAYYCM010000000
VERSION     DAYYCM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.168"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..978)
                     /locus_tag="ACHAY1_002922"
     CDS             complement(<1..978)
                     /locus_tag="ACHAY1_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM8594840.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKF"
     gene            complement(1230..>2088)
                     /locus_tag="ACHAY1_002923"
     CDS             complement(1230..>2088)
                     /locus_tag="ACHAY1_002923"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8594841.1"
                     /translation="NNSILGMKIRNKINQLNQMKKRDNLLPAKYTLKSMTGKGPIKDY
                     SNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIKEPDFSHPLVDYYLYTDNVIT
                     SEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYDFTLYVDGNVTITSDITGLLN
                     SSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIEQISKYREEGFPDNFGLAEAT
                     VILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVLWKSGHAMKEVTTLGNNVYKS
                     AKLRIISHY"
BASE COUNT          661 a          389 c          281 g          757 t
ORIGIN      
        1 gaatttttta atgccctcat ccaaggttat attcggttta aagttgattt tttcctctaa
       61 atcttcaata tcggcataag ttctaacgac atcacccggc tgcatgtcca aaaaaatctt
      121 gttcgcttta acacctaaat acttttcaat tgtttcaaca aactccaaca atgcaaccgg
      181 cttattattt ccaatattgt atatcttata tggtgcccaa ctttcactac tcaaatcatc
      241 gatgtctgtc cagtcggagt tgggttcagg tactttattg attaatctag tgattccctc
      301 tacgatatca tcgatgtaag tgaaatctct ttccatcttt ccatggttaa aaatcttaat
      361 ttcttttcca gataaaatat tcttggtaaa agagaaatag gccatatctg gtcgcccaaa
      421 tggaccatag acagtgaaaa atctcagccc agttgttggt attttataaa ggtggctata
      481 agtatgagcc attaattcat tcgatttttt ggtagccgca taaaggctaa ctggatgatc
      541 caccgaatct tctgttgaaa acggtacttt cgtgttccca ccatatactg aactagatga
      601 tgcatataat agatgcttta cagggaagtt tcggcaggct tcaagaatat taagaaaacc
      661 gataatatta gaatcaatat aagcataagg gttttcaata gaatatctaa cacctgcttg
      721 agcagctaaa tgaattacta tgtcaaaacc ttcactttca aatagagaat ctaatttttc
      781 tttctctaat agatccattc tgataaattt aaaattatta taatcgtaca gtatcgttaa
      841 tcgatcttct tttaattgga catcataata atcattgaga ttatcaatcg ctacaatttg
      901 attttcagtt tccttgagta attttctaga caaatgaaaa ccaataaatc ctgctccacc
      961 tgttattaat attttcattt ttattcatcc acctttttct gaaaggaacc aatagggtta
     1021 aacccaaaat tgctttcttc tttatactcg tattacattt attatttagg cataccccat
     1081 aatttaagat ataacgaagt ttatgatact atgcgtcttc tccattcact caaaaaatat
     1141 aacttaattt tttatgactt taaaaaggaa atcttctatc tccaaatatt tactaggatt
     1201 tgaaagaata gtttagaaat catttagggc taataatgac ttatgattct taatttagcg
     1261 cttttatata cattattgcc taaggtagtc acttccttca ttgcatgtcc ggacttccac
     1321 aacacgtaag gaaatgcgag ctgatctcgc ccactttctg agttcaaata ctctctccac
     1381 cacaaatcta gaagattctc agcgattgaa ttatttaaat caactaaaat aaccgtggct
     1441 tcagccaatc caaaattatc aggaaatcct tcttctctgt acttactaat ttgctctata
     1501 atactttcct tattaccttt gccaactgca attaatgcat ctgcctcaga atataaagat
     1561 tttctttcac tatgtttatg catacttaac ccaagtttgg aagaactgtt cagtaatccg
     1621 gtaatatcag aagttattgt tacattacca tcaacataca aagtaaaatc gtagtcagta
     1681 aatagctcat taggatgaaa tttaatatat ctattgatca aaatattatt ttttaattcc
     1741 gatatatatt taggtatctc tctaatattc caatgctcac ttgtaatcac attatctgta
     1801 tacaaatagt aatctaccaa tggatgagaa aaatccggtt ctttaataga atcataatta
     1861 ccagtgatac aactatacac agcaatcctt ttaggtgttc cttgattagt taactctttt
     1921 tttcttagtt catcactgcg attctctctg tttgaatagt ctttaatagg ccccttaccg
     1981 gtcatcgatt taagggtata ttttgctggc aacagattat ctcgtttttt catttggtta
     2041 agttgattaa ttttatttcg tattttcatg cctaaaatag aattattt
//
LOCUS       DAYYCM010000162         2040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000162 DAYYCM010000000
VERSION     DAYYCM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.169"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..424
                     /locus_tag="ACHAY1_002924"
     CDS             <1..424
                     /locus_tag="ACHAY1_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8594842.1"
                     /translation="LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIK
                     DYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKD
                     VHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(555..1031)
                     /locus_tag="ACHAY1_002925"
     CDS             complement(555..1031)
                     /locus_tag="ACHAY1_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8594843.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(991..1347)
                     /locus_tag="ACHAY1_002926"
     CDS             complement(991..1347)
                     /locus_tag="ACHAY1_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594844.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1325..1510)
                     /locus_tag="ACHAY1_002927"
     CDS             complement(1325..1510)
                     /locus_tag="ACHAY1_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594845.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1511..1783)
                     /locus_tag="ACHAY1_002928"
     CDS             complement(1511..1783)
                     /locus_tag="ACHAY1_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8594846.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1780..1980)
                     /locus_tag="ACHAY1_002929"
     CDS             complement(1780..1980)
                     /locus_tag="ACHAY1_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594847.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT          608 a          388 c          291 g          753 t
ORIGIN      
        1 tttaccagaa aatattgaaa aagtttctcc tacatttgtt gatatttata ctcaagccac
       61 acaagctaaa tctgagcaat tggaacaaat tgctggagtt ggttatagaa aagctctaga
      121 atttctaatt aaagattatg caatatctaa aaattcagag gatgaagata aaataaaaaa
      181 aatgttatta ggtaaaacaa ttgaaaatta tttgaatgat tttcctaaac tccaaagatt
      241 agccaaagct gctacttgga ttggtaatga tgaaacgcac tatgttcgta gacatactaa
      301 taaagatgtt catgatatgg atgcatttat taaatcagca gcgcaattta tcgcggctga
      361 ttatgatgct gatttagctg atcaatttat aaatggaaat aactttatgc caactactaa
      421 ttaaaagaat aaaagagcca cagataccaa ttaataaaac ggttgggata tcttggctct
      481 ttcatatttt tttgacacta atagaatatc atgctgctaa caatgtttac gaacatttag
      541 tgaacagtgt aattttagaa taaagagagt tgttctttgt acttctcgct attgtaaaaa
      601 tcaatttcgt tttctttttt caatcggtca gcttgtctgt gttcatactc atctaaaaag
      661 ttcaatgtct ttctaatttc tgcatgtcgc tgtctgacat acgatgagct atacccaaca
      721 gattcagcaa tatcttctaa gctcatttga tcaacatatt tcatcttaac aatttgatta
      781 tcgataccag aaaagctatc tatcagcaat aacatttctt ctttttgctc gatcaacaat
      841 tccaattcat tttctatctt ttggatattc tcttctaaag aagaagttct cgaattcttt
      901 tcgatgcgga cgtttgctaa atcaccacag acccatcgat tcaattcaag cttactttta
      961 ttaagattcc actttaagta aagaatctgc tcatctagtt cttggtaatc ttttaaccaa
     1021 tgaaatctca caaacgccac cccttatggt agaatagtat tgcgctgcta tctcgaaagg
     1081 ggtagctttt ttattttttc ttagccgttc cattctggat tgccttttgt tccaaacgtc
     1141 gttttttctt cttaatcttt gattttgttt ttcccataca attctccttt ccgaaaacac
     1201 attttgttcg atttttaata ctaaataaaa gttggccaga atttttcgtt cgtctttact
     1261 catctttttt tgaataagat tactagcata aaaatagtta gtaccacgaa aatgaacagg
     1321 taaatcaatt acgttcacct tcttcatgcc agttatcagc cactagaatc tttgcgttag
     1381 tgccgccacc gttttctttt ttgtattttt caaatgcttt ataagcagct gtttttgatt
     1441 tatagatttt tccggaaccg actgggaaca tccatgtgcc ctctgtgata gctaagatgt
     1501 atactttcat ttaattcacc cctttaatcc ccaacactac atatccggac ttctgcatgt
     1561 aatcagtaat ataagtgatc tctgctttgt atgagtcacc agtgtaacta aatgttggtc
     1621 tcgttagtcc aggtaattca tgtttaatgt attttttata ttcacgtagt attaactgat
     1681 caccaacttt gaagtcacga tcattcttgc ggatctcgaa ttgtttgcgt ccgctcgtca
     1741 ctgcttcaaa atattctggt agtattttta gttcgtggat cattctgttt cctccttcaa
     1801 aattcgctca tcatcaaaaa gtgaatctcc acaaaatcct tctcccaaat atttatcgtt
     1861 catgtataag tcaaccaaat atttgtgatc tgcttctcga tattcattaa tattaacgat
     1921 ctttattttg taaacattac cttcgtcgct cactatcttg aattgttctc tgactttcac
     1981 caccgttcct cctctttttg aaaaaatttt agtgtttttg taagaccccc taaatcagct
//
LOCUS       DAYYCM010000163         1987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000163 DAYYCM010000000
VERSION     DAYYCM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.170"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..803)
                     /locus_tag="ACHAY1_002930"
     CDS             complement(<1..803)
                     /locus_tag="ACHAY1_002930"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8594848.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFS"
     gene            complement(775..1671)
                     /locus_tag="ACHAY1_002931"
     CDS             complement(775..1671)
                     /locus_tag="ACHAY1_002931"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8594849.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1661..>1987)
                     /locus_tag="ACHAY1_002932"
     CDS             complement(1661..>1987)
                     /locus_tag="ACHAY1_002932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594850.1"
                     /translation="KVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDT
                     KEIVKRYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYK
                     VMNDEK"
BASE COUNT          696 a          334 c          244 g          713 t
ORIGIN      
        1 gaaaatatat atgagcttaa gctgataact tttttttcgt aaatattcat ttttgataat
       61 ttatgaacta aaaaaagatt tgctgaaaaa aagagagcaa acatcactac gtgagaaatc
      121 gatgtactgt ttctatcaaa atatgcatca tacttcgtat ttataaatat tgaactgata
      181 taactttgtc caatagagaa taggagcaag ctaaggattg taacatatcc agtaatcttt
      241 ttattaattt ttacaataaa aaaagggaga taaaaatatg caagattatg gaacagtcca
      301 gccactaaga taaaaaaaat atacttaaat atagctttgc tgcctcgcaa tttagttaga
      361 tgataaaaag ctagtaatat gaatatcaat gcgctaaaca tacgtacttg tattgcttgt
      421 gaagtaaaac caaaaataaa aaacaagaac aaaactaaaa aaacattagt tgtgaataat
      481 ctgatgacat acaataaagc tgagagtaag acagtagaaa taatcaataa aaaaatctga
      541 tactgactgg aaataaaggt acatacgtac ataacagctc tgaatcccgg ttcaatacta
      601 tctgaactaa agtccatacc aaattgtgct atcccattat acaagacaga atatgcataa
      661 taatcagcgt tttgtgtgtt aaatgccatg atgatccaca ttatcaaaag tagcacccca
      721 gacaaaatct tgttcgatgt actccatttt ttttgtgaaa cactcttttc ttgattaatc
      781 tccaatgacg cctctgtatc catctcgaac cccccttata gccgctctaa atctactgag
      841 cttatcagtc tcaaaagcta ttgcaataaa aaatttcaat aatcttttat tcataaggcg
      901 ggaaaattcc ttatcacttt tatacattct tttcaccaat gttgaatttc ttgcaataaa
      961 atactttcgt gttgaattat gattgtctat tttaattttt tttgatccta tttttaaatt
     1021 ttctccatct ccaacgtgat gataaaaata gacttgattt gttacataaa tgttatagcc
     1081 attatttgca acccttttac aaaattcaac atcaatctga tcaataaaaa attcatcctt
     1141 catatttcca acatcgtcaa ttaattcact tttgaaaaac attccagagg aatttgcaaa
     1201 tgctattttc atggaaccac tagttgaagc aaagtgtttt tttacgtatc tccctctctc
     1261 atttttgcag gtaaaagaga cagattcatc attgctattc tcaaagtaat tgggaacaat
     1321 aatcccaatg tccttatctt tcgcttcaat attggtaaac tcttctaata attttaaaga
     1381 aagattcttt tgtatgatgg tatcttgatc tagtaaaagg atataatcac atttctcttt
     1441 tttggcaatt tcaataccta tgttttgagc tgtcgcaagc ccaatgttct cattcatttt
     1501 ttttaaaatt aagttttcca tcatttcaaa ttgaaaagag ttaatattat ctgagccgtt
     1561 atccacgata ataagtttgt tctgtttctc ttgtattaac gtatccacta ataactttat
     1621 gttattatct ggattgtagg taacaataat tatcccaatt ttatttttca tcgttcatca
     1681 ccttgtacac ttcattccat ttcattttca atacttgctc agagaatata gtggcgtaaa
     1741 tttttctaac attttcattt atgtttttat tacactgata aaatttcaac atctcatttt
     1801 catcaacggt actattataa ttgattccga cattatatct tttaactatt tctttcgtat
     1861 ctcctgataa atttgaaatt ataggtaatc caaacctaaa atactcaatc gatttcagac
     1921 ttaaaccaat tatggtattt gacttaaata aatttagtcc aaaatgacat ttatcgagta
     1981 taacttt
//
LOCUS       DAYYCM010000164         1917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000164 DAYYCM010000000
VERSION     DAYYCM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.171"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1546
                     /locus_tag="ACHAY1_002933"
     CDS             122..1546
                     /locus_tag="ACHAY1_002933"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8594851.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            1632..>1917
                     /locus_tag="ACHAY1_002934"
     CDS             1632..>1917
                     /locus_tag="ACHAY1_002934"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594852.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVN"
BASE COUNT          606 a          251 c          341 g          719 t
ORIGIN      
        1 tagctattca catattcaaa aaaattttta ctacgaggac tcacacgata cagaggaatt
       61 taggtagttc taattaaacc agaaagtcat ctatagatgt gagttgaggg aggtcatacg
      121 gttggatagt agcagtaata aatcacattc aatagctaaa aaagctttta tttacgcaat
      181 aggaacatta tcatcaaaaa tgatatctgt cattataatc cctatctatg cagtatatat
      241 ttctgttgcc gatttaggaa gctttgatta tcaaaataca attgctcaat ttctttctcc
      301 agttattttt ggagcaatat gggaagcaat tttacgcatg ggattaggca aagaaaaaaa
      361 tattcagaaa aaagtagtga cgaacagtat ttgttttgtt atagcaaata cagtgatatt
      421 agccattttt ttaccaataa tatatagaat gattttcccg aatttagagt attatcaatt
      481 gtttgttttt caattaattt tcactcctat cctaagtgtt tttcaatata ttgtaagagc
      541 actaggtgaa tcaaagattt ttgtgatgag tggtataatc gctgcgggta tcaacctgtt
      601 actccttact atttttgttg ttacaatggg tatggggata cttggtctct tactctctat
      661 cataatttct aatgttataa atttgatttt tttgattttc agggggcaga tctttcaata
      721 tttcgcaacg agttcttttg attttttatt tttaaagtcc atgctgaaat actctatccc
      781 actcactttg aatctcactt ttggatggct aatagttggt ttttcaagat tttatattgg
      841 aacgtacatc ggagtaacag aaaatggtat tttttctttt gcgatgaaat tttcatctat
      901 aattttaact cttacaagta ttgttagtat gtctatgata gaggatgcgg ttatcacgtc
      961 tgagaatgat gatttcatcg aaagattcgt tgagaaaaat acctatattt ttaaaatgta
     1021 cattaggtta gcgactcttt ctattcctat catttgctgt gcatactctt ttataaccaa
     1081 ccctgattat aaaaaatcat tagttctagt tccaatatta gttattatgg catgtttcag
     1141 tgggatgaca acaaatatcg gaaatatatt tgcagtatac aataaaacaa atcagttatt
     1201 aatgatttca gtagtgtcag gtctattgag tgttgttggc acctatttat taggctattc
     1261 ttttggagta attggtgtct catttgctac tttgctcggt actttagtat tatttggact
     1321 tagatattat tttggaagga aaattgctta ttatgcattt gattggaagc gaatatcgct
     1381 acttttagtt ctctattccg caatcgcttt tgtatgcatt catagaggct tctattttaa
     1441 tcttttctta acgattattt ttagtgctat tttcgttttt acgtataaac aattaattat
     1501 aacgtatcta aaaaaatttt tcagaataaa aatggctaaa aggtgagggt atacgaagta
     1561 agaatagaat ctttcgaaga ataaagagaa aactatttgg aaaaaatctg cgagaatgta
     1621 ttaatgaagg tttgatattc ggaaaaaaac atagtttagt agaggttcct gaatttggga
     1681 gcgagccata tttaattgaa ataggggata atttcagatg ttcatttggt gttaaattta
     1741 taacacacga tggtggagtc tgggtcttaa ggaagatgag ttctgtgagc gagtcaactt
     1801 caaaaatagg taggatcaaa ttgggaaata atatttttat tggtatttac tctattgttt
     1861 tcccaggagt ttcaattggc gataatgtaa ttattagagc gaacacttta gtaaata
//
LOCUS       DAYYCM010000165         1848 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000165 DAYYCM010000000
VERSION     DAYYCM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.172"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1612
                     /locus_tag="ACHAY1_002935"
     CDS             20..1612
                     /locus_tag="ACHAY1_002935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8594853.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            1605..>1848
                     /locus_tag="ACHAY1_002936"
     CDS             1605..>1848
                     /locus_tag="ACHAY1_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8594854.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          642 a          300 c          392 g          514 t
ORIGIN      
        1 gagataggag tgtatctttt tgggtgcaga attaaatcaa aaattattta gtgcggcaga
       61 taatctccgt agcaaaatgg atgcgtcaga atataaaaat tatttattgg gactgatttt
      121 ctataaatat ttatccgacc gtttgttgga acaagtggtt cttttagcag atgaatcctt
      181 agaggaatat gatacggtaa gcaaacaaac gatgttgtat cgcgaattac tttcagatga
      241 agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg cgatttcgcc
      301 agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaatga
      361 tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga
      421 tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat
      481 taccgaagtc attaagaaat tgaatgacgt ggaagtttta ggtcacgatg gggacgtgat
      541 cggggatgct tatgaatttc tgatcagtca atttgcttca gaagcaggga agaaagccgg
      601 agaattttac acgcctcata tggtttctga tatgatggca caaatcgtga cgttggatca
      661 aaaagaacga cgattcttta gtgtttttga tccaaccatg ggttcaggtt cattgatgtt
      721 aaatgtgcgt aactacttaa ctcacccaga taacgttaaa tatcatgggc aagaattaaa
      781 tacaacgaca tacaatctag caaaaatgaa cttgatttta catggggtag atgccgaaga
      841 aatgaatctc cgcaatgggg atacgttgaa taaggattgg ccaacagatg aaccttacac
      901 atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacctt
      961 cttagacgat tctcggttta atcgttatgg aaaattagcg ccaaaatcca aagcagattt
     1021 tgcgtttctt cttcatggtt tctatcattt gaaggaaaca gggactatgg ccatcgtctt
     1081 gccacacgga gttttattcc gtggcgctgc agaaggggtc attcgtcaaa aattactaga
     1141 agacggcagt atttatgctg tgatcggtat gcctgcgaac ttgttcttcg ggacatcgat
     1201 tccaacgacg gtgattgtat tgaaaaagaa ccgccaaact cgagacgtat tattcattga
     1261 cgctagtcga gaatttgtca aaggaaaaaa tcagaataaa ttatcggaag aaaacattca
     1321 aaaaatcctc gaaacctatg ctgaaagaaa agatgtcgag aaatacgctc atttggcaaa
     1381 ctttgatgaa atcaaagaaa atgactataa cttaaatatt ccacgctatg tagatacgtt
     1441 tgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca
     1501 agaacagcaa gtgctagaaa aagaactttt agaagctatc tcttccctgc aaacaacacc
     1561 tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc aaacatgagt aatgatatac
     1621 aaccagaaat tcgtttccca ggatttacgg aagcttggga acagcgtaag tttgaatgct
     1681 tacttgataa aaaagatgga gtaaggaggg gaccatttgg gagtgcctta aagaaagaat
     1741 tctttgtatc taatagcaat tttgttgtct atgaacaaca aaatgctatt tatgataatt
     1801 acgaaacaag atataaaatt actgaaaaaa aatataatga attaatca
//
LOCUS       DAYYCM010000166         1711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000166 DAYYCM010000000
VERSION     DAYYCM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.173"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACHAY1_002937"
     CDS             complement(<1..161)
                     /locus_tag="ACHAY1_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8594855.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEIS"
     gene            complement(158..811)
                     /locus_tag="ACHAY1_002938"
     CDS             complement(158..811)
                     /locus_tag="ACHAY1_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8594856.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1099..>1711
                     /locus_tag="ACHAY1_002939"
     CDS             1099..>1711
                     /locus_tag="ACHAY1_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8594857.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          548 a          311 c          234 g          618 t
ORIGIN      
        1 ctagaaattt catttgccat attatgaatc ttttcttttc tcgtctccaa atatgtttta
       61 ggaaagataa aaaaaattaa tgaatgaact aatataatta ttattccaag aacagaaaat
      121 atttgtatga acatttttgg aaatatcttt aattttttca ttttctctcc aatttatatc
      181 ctacatttct tatagtagtt atacagtcta attgtaattt ttttctaagt tcttttatat
      241 atacatctat tactcgatca taaggagttt cttcgctatc tttccataca taatcaataa
      301 tttgcattct tgttaacact tgtccattat tatccaataa acattttaat acttctagtt
      361 cttttgcatt tacatctatt tcttcaccat ttattttagc tgtatagcta ttaaaattaa
      421 ccgatagatc cttatattca aacttctcta aatgtccata attcttctta attaaagaat
      481 ctattctagc ttttaagacc ggcaatgaaa atggcttttc tacatatccg tctactaaat
      541 tagtaaatgc atcaatttta tattcttcat cactaaatgc agtcaagatt agaattggta
      601 aattgctttt ctttctaatt tctttcaaca cttctaaacc atttataaaa ggtatctgaa
      661 tgtctaagat aaccaaattt atatcgctat taaattttga caaggcttct cttccatctt
      721 tagcttgaat aacagtatat ccaaattctg aaagatattc acttattccc tctcttatca
      781 tattatcatc ttcaacagtt aatattttca tatatacctc cacttaagcc tatacactag
      841 atattataca gcatattatg gctctataat atctagtgtt ggtgacacct tctgttgcca
      901 atactttttc ttttttctaa taaatgcaaa cagacacctt gaaatccttt agaataaagg
      961 gttctataat attttgtgtt gttggaaaat cgaaggcgat tttctactaa cactttttct
     1021 tcgtttcaac tcaaaaagag actcactttt ggtaaaattt agtcgaccaa aacatcaccc
     1081 taccaaagga gtcgctctat ggataatcat actagaaaat tactcgtatt aacagataaa
     1141 catttatttt ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag
     1201 tttattaaag ggagattgga tgactcacct tcccattgtg aacactgtgg atcgattcgt
     1261 attatccgca acggttccta tacgacacgt actcagatat tgaaagtcaa agaaaagttg
     1321 actattttag aattaaaacg tacccgtttt ctatgctatg attgtgggca gaccttttct
     1381 gcgaaaacgg acttagttga tgaacatcat caactaacaa aggaactgaa gcaggcgatt
     1441 ctcatagaac tgtacgaaaa tcaatcacgt aaactaatcg ctaagaagta cttcgtctcg
     1501 gatgggacgg tgacacggat cttgcgtgaa gcaacgaagc attaccagcc acgtatgaac
     1561 ttccttccga cggtgttgtg tatggatgag ttcaaatcaa tgaagtctgt ttctggttcc
     1621 atgagtttta tttgtgttga tgggacaacc catcagctat ttacgatcct tgaagatcgc
     1681 agactttaca aactaactca atacttcctg c
//
LOCUS       DAYYCM010000167         1694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000167 DAYYCM010000000
VERSION     DAYYCM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.174"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACHAY1_002940"
     CDS             <1..544
                     /locus_tag="ACHAY1_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594858.1"
                     /translation="IPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGID
                     ETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLI
                     SPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEE
                     KILLPENWAELRKLVEYGII"
     gene            531..>1694
                     /locus_tag="ACHAY1_002941"
     CDS             531..>1694
                     /locus_tag="ACHAY1_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8594859.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAA"
BASE COUNT          555 a          220 c          273 g          646 t
ORIGIN      
        1 aattcccaaa atagatgaaa acagtaaatt gatgaaaaac atacaatcaa tctttgggat
       61 agaagaagtt tttgtcggag ataaatgtat cttttttgat caacctttat tatctgatgg
      121 ttatggcata gatgaaacag atattttcgc taaagtaaat agtatatttt ctggaaaaaa
      181 agaaacttta attaagcttc atcctagagc aaggacaaat cgttatggtg aaaaaaacat
      241 attgaattcc agtttaccgt ttgaagtctt gctacttgaa aatgacttct cttcaacact
      301 tttgattagt cctatatcta caatttcttt ttcaccatct atgatgtttg aaaagacgat
      361 caactctatt atgttggcta aattaatttt aaatgaatat acagcattat ctgaagataa
      421 aaaagaaaca ttgacaaata ttgttctttt ctgtgaacat tataaccgtc tatcagaaga
      481 aaaaatttta ctcccagaaa actgggcaga attaagaaag ttggtagagt atgggattat
      541 ttaacacact ttccaagtca aaattagtta agtccagttt ctggtatact atagggaatt
      601 tttttattaa gggaatttca ttcataacta ttcctatttt tgtaaatatt actactcaag
      661 cagaatatgg tttgattaat aatttcactt catttgtatc aattttttcg attcttgttg
      721 ggctaagcct aaattcggca attaataatg caagttttga ctataaagat gacataaatg
      781 aatttatgtc atcggtactt tctctagcta cactttcgtt aattgtgttc ttattaatag
      841 ggaacatata ttttttgttt tcaaatgctt tttttgatat gaaccaactt gtatttaatt
      901 tgatgttatt acaaagtttt gctagttttg taattagtta tatccagtct tattttacga
      961 ttaactttaa ccattttaaa tatttgattt tatcttttat atctactttt ttgaatattg
     1021 caatctctgt agctttgatg cagacattat attttgatga taaatatgtt ggcagagtag
     1081 tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga ataatgataa
     1141 agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc
     1201 taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt atgattaatt
     1261 cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata ggtattattc
     1321 taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat ggtgagatga
     1381 gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta gtctttactt
     1441 tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg gctccgaaag
     1501 actattattc tgggttatct ttagtggttc cgataacctt aggctatttc tttcagtttt
     1561 tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt attgcgattg
     1621 gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata cctaggttag
     1681 gaataattgc tgct
//
LOCUS       DAYYCM010000168         1682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000168 DAYYCM010000000
VERSION     DAYYCM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.175"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHAY1_002942"
     CDS             200..742
                     /locus_tag="ACHAY1_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594860.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACHAY1_002943"
     CDS             898..1347
                     /locus_tag="ACHAY1_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8594861.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>1682)
                     /locus_tag="ACHAY1_002944"
     CDS             complement(1377..>1682)
                     /locus_tag="ACHAY1_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8594862.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          636 a          240 c          324 g          482 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gt
//
LOCUS       DAYYCM010000169         1655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000169 DAYYCM010000000
VERSION     DAYYCM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.176"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHAY1_002945"
     CDS             <1..1412
                     /locus_tag="ACHAY1_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8594863.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1655
                     /locus_tag="ACHAY1_002946"
     CDS             1546..>1655
                     /locus_tag="ACHAY1_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFM8594864.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKS"
BASE COUNT          656 a          251 c          355 g          393 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcaga
//
LOCUS       DAYYCM010000170         1647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000170 DAYYCM010000000
VERSION     DAYYCM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.177"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHAY1_002947"
     CDS             <1..1604
                     /locus_tag="ACHAY1_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8594865.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          604 a          269 c          336 g          438 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagta
//
LOCUS       DAYYCM010000171         1637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000171 DAYYCM010000000
VERSION     DAYYCM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.178"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1316
                     /locus_tag="ACHAY1_002948"
     CDS             <1..1316
                     /locus_tag="ACHAY1_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8594866.1"
                     /translation="VDIFKQVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNES
                     FELLFSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISKD
                     IEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEKI
                     DRYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDIDGLYDNNVEELVTVAA
                     ITIDDFDYYIRGHQKVSSLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTST
                     VQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNV
                     IWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLD
                     NPDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            1451..1579
                     /locus_tag="ACHAY1_002949"
     CDS             1451..1579
                     /locus_tag="ACHAY1_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8594867.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          630 a          215 c          312 g          480 t
ORIGIN      
        1 tcgtagacat attcaagcag gtgttaaatc taagcttaga tttgaatttg attgaattgt
       61 taatttcccg agatacggaa cataaacaaa gaaaaataac tgaagaattc ggtgtagata
      121 agtggaatga atcgtttgag ctattgttta gcgaaaatgc gttaaataaa aaagttaatg
      181 actttttgtt atcaaatgga ctaaatgaag aggaatatcc taccattttt gaaattgact
      241 attcatatgc attaaaagaa gaagagtatc aattgttggc gatagctcta gaaagcattt
      301 caaaagatat tgaggatttg aatggattgt cagaattgat tcatttggat attcgtgaat
      361 tttggaaact gaaattaaga tcttatctag ataagaaagc ggaacattac cgccagattt
      421 tatatagcaa tgcaaataaa agcggtgatg aaatactcaa agaaacattc ctagaaaaaa
      481 ttgatagata caaagattat caatttgaga tttctgaaat tccaaattca gttcaagtcg
      541 acattgagaa agaaagtaag aagagatttc cagagctaag tgtgtcaatt actgatgaaa
      601 ttgacattga tggcctgtat gataataatg tcgaagagtt agtcacggtt gcagctatta
      661 caatagatga ttttgattat tatattagag gtcatcaaaa agtttcttcc ttactgtatt
      721 ttggtattcc gtcccgttta agagaagata tactgatatt cgttactaaa gaaaatgatg
      781 ttgatttgaa cggagaaaaa tctggtatct ctattgaagg aacatcaact gttcaaacca
      841 ttcttgaacc atcgaatagt gttagtagga aaggtaagaa cacacgaaat ggagaaagaa
      901 gtaaacgaga ttatgataat agaaactccg caaatgatga tgctggtgaa acagcagaac
      961 aaattgctta cgctgaattg actcagaatc aaaaacttaa tgtaatttgg aatacgaaat
     1021 attctagtaa atcttcagat cgtaataagc agccaccaaa cggaattgtt tgtgatatgt
     1081 gggttcatga cccagagaat gggaatatgt actttgaggt aaaatcatca attacagaat
     1141 ttgaaatgtc gattaatgaa tataattcta tgttagataa ccctgactct tacgaggtcg
     1201 ttttagtcaa tcgtgatacc aaagagataa gtcgtcataa atttgatgaa cttgatggat
     1261 tgaaacaggc aagtagctat cttttcaaat ttaagcaaaa gagaagtgac ggttgagtga
     1321 tttgtttcca caaacctatt caatctatca actcaaaaat cataaacaac cattattata
     1381 gtaggaagat acagagtgta ctctcaacgc atatcgaatt agtcgcttca ttcgttaaag
     1441 gagaataaaa atggaccaaa aagaaaaaat gaaagaattg atcaaaaaga aacaaggtgg
     1501 aggccgatcc aaacaaatgg aaacacctaa gaatgatcgt aaaaacatga gaaaaggccc
     1561 taaaatcttt aataaataga cgtgctaaag aaagactaag aagtgttaac ttttgttatt
     1621 gtttcttagt cttttta
//
LOCUS       DAYYCM010000172         1586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000172 DAYYCM010000000
VERSION     DAYYCM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.179"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..241
                     /locus_tag="ACHAY1_002950"
     CDS             <1..241
                     /locus_tag="ACHAY1_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594868.1"
                     /translation="LGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEIL
                     EKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(603..842)
                     /locus_tag="ACHAY1_002951"
     CDS             complement(603..842)
                     /locus_tag="ACHAY1_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594869.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1128..1442
                     /locus_tag="ACHAY1_002952"
     CDS             1128..1442
                     /locus_tag="ACHAY1_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594870.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          541 a          216 c          279 g          550 t
ORIGIN      
        1 tttaggaaaa aaatatattt gccaacctat tggcttgaaa caaaaagtga ttggagaagt
       61 aatcagtaaa ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg
      121 caatgaaata ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt
      181 ggaaacaaca gaatatctat ataactatag caacaaatat gaacatactt tagtttcata
      241 aagatttaaa taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt
      301 agttggcagt gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt
      361 ctctgtaacg aggcctggtg gagaaactcg ataaaaacct atactgccag aactgtccta
      421 ttttcggata gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa
      481 tgcaaaactg atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg
      541 ttgcatttag ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga
      601 agctaattca gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt
      661 tttgcagagg tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta
      721 gaaattgttg aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca
      781 gaagtcccac aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc
      841 attatgtaca cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa
      901 atcaatgcaa aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg
      961 agagggtgat atcccagttc aaaccagata cattggttac catctgtatt aacgattatg
     1021 cctctaaacg ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct
     1081 agaggtaatt tttttgggta tttaaatagt tgctaggagg atagcttttg gatttttaca
     1141 gtaattttat cttaataata gcaatattat tacttctaaa tatttggttt tttgataaga
     1201 gtagaaatgc agggataggt tttagaacca aacgaagtac gtcttctgaa aaaaagtggg
     1261 tatattcaca gactattttt tacggagggg ttatttcaat cagtttactt tcttcaacac
     1321 tttattcttt taatgttata gacgtttcca tgtcaaattt tatatctatt attggaatcc
     1381 ttatttctgc aattattaca caactacttc ttgtgtttga agagaagtca aaaaataatt
     1441 aaagcaatta tgccttttag tacaggaatt attacttctt acaaatgtat agcgaatgtg
     1501 aagcctgctg ttttaattgt tctagaaaaa cttttgagtg aacaattatt ttatgttagg
     1561 atgaattaat ttctctagag tttata
//
LOCUS       DAYYCM010000173         1506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000173 DAYYCM010000000
VERSION     DAYYCM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.180"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1461
                     /locus_tag="ACHAY1_002953"
     CDS             <1..1461
                     /locus_tag="ACHAY1_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8594871.1"
                     /translation="KQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVA
                     NGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLA
                     LPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALL
                     APLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKG
                     FIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAE
                     EKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIF
                     IFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWL
                     LGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTP
                     GTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          411 a          289 c          318 g          488 t
ORIGIN      
        1 aaacagcaag acaagggctt cttcgggcag ccgaagggat tgtcaacttt gttcttcact
       61 gagatgtggg aacgatttag ttattacggt atgcgtgcat tacttatcta ttatatgtac
      121 gatacagttg ccaacggggg acttggatta ccacgagcaa cagcattagc tatcatgtcg
      181 atctacggat ctttgatcta catgtctagt atcgtcggcg gatgggtatc tgaccgagtg
      241 ttaggagcaa aacgcactgt attcttcggt ggagttttca ttatgcttgg ccacatcgtc
      301 ttggctttac catttggtgt aagtacattg tttgtttcga tggcattgat tattatcgga
      361 accgcctttt tgaaaccaaa tgtttctggc atggtcggac atctttattc aaagactgac
      421 ttgcgaagag atgcaggatt ttcgattttt tacatgggtg tcaacttagg ggcattatta
      481 gcaccactaa tcgttggtac aatcggtcaa gaatataact atcatttagg gttctcttta
      541 gctgctattg ggatgttctt tggattatta caatacatca tccaaggacg caagacatta
      601 gacggagtgg gtacaacacc gccgaatcct ttgacaactg aagaacgtaa aggattcatc
      661 cgtaacttga tcatcgcact tgttgttatc gctgcagtat tcggcggggc acaactaact
      721 ggtcatctga caattgactt ttttgtcaat acaatcagtg tattggggat tatcttaccg
      781 ttgtattact ttatcaaaat gctgacttca aaagatgtaa ctgctgaaga aaaaccaaga
      841 gtatgggctt atatcccatt attccttgca gcaatcattt tctggtcatt acaagaacaa
      901 ggttcatcta tcttagcttt atttgctagt gaacgtacac aatctacgct atttggtgta
      961 acgatccaag caagctggta tcaatcattg aatccaattt tcatctttat tctgacacca
     1021 gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac catctacagt agtgaaattc
     1081 tctcttggac ttttatttgc aggattatcg tacttgctat tgatgttgcc gggtatgctt
     1141 tatggtacaa gtggaaaagt aagtccattg tggttgttgg gaagtttctt catcgtggtt
     1201 attgctgaat tgtgtctttc accagttggt ttgtctgtga caacaaaatt agcaccacga
     1261 gcatttgaat cacaaacaat cgctatttgg ttcttagctg atgcttcttc acaagcaatc
     1321 aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat ctgcttactt cggtattatc
     1381 ggtgcagtag caatcgtcgg tggtatcttg ctatttactg tgaaagaacc aatcaaaaaa
     1441 ctaatgggct ctattcatta atcaatgaac ataaaaacag gaattgctta tttgcagcag
     1501 ttcctg
//
LOCUS       DAYYCM010000174         1489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000174 DAYYCM010000000
VERSION     DAYYCM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.181"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..514
                     /locus_tag="ACHAY1_002954"
     CDS             104..514
                     /locus_tag="ACHAY1_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8594872.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            528..884
                     /locus_tag="ACHAY1_002955"
     CDS             528..884
                     /locus_tag="ACHAY1_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8594873.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          548 a          210 c          273 g          458 t
ORIGIN      
        1 aataatcagt tattttgatt ttttttatat tttattagct aataattggt attttaaaag
       61 aaattactgt agaattggat tataattaaa aaggagtgag aaaatgataa aagtatatac
      121 ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga
      181 atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt
      241 ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa
      301 gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata
      361 tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa
      421 tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat
      481 ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag
      541 tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc
      601 aattgcctta tcaatttgtt gacttagaag aacgcgattt aagtacagaa gaagcagaag
      661 aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt
      721 ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca
      781 cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg
      841 gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa
      901 tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta
      961 tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa
     1021 aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg
     1081 atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt aaaaaacata
     1141 taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagttg ttaaaaaaga
     1201 accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca
     1261 aatgataagt tatgataaat gagttgctgt aagatttcga aaaacgaaat aaaaaaaact
     1321 tttaaaaaag agttgacaat tacttgtctc tctgatatga tataaaagtt gctacggcag
     1381 ccaaaaacga atcggggaac actcctgatg aagtagacct ttgaaaactg aacaaagtaa
     1441 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaaca
//
LOCUS       DAYYCM010000175         1489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000175 DAYYCM010000000
VERSION     DAYYCM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.182"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..>1489)
                     /locus_tag="ACHAY1_002956"
     CDS             complement(12..>1489)
                     /locus_tag="ACHAY1_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594874.1"
                     /translation="SIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGY
                     NLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAM
                     SILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKK
                     KIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLI
                     GSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQ
                     NTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYS
                     SVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYS
                     SFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGI
                     NKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          573 a          199 c          204 g          513 t
ORIGIN      
        1 gtgctagcaa atcattcatg gcttgttctt atttttaatt ttatgcaata aaaaagaaga
       61 acaattagcg tgattataga aataaataat gagattttag aaaaaaatgt gtatgaatac
      121 ctgattgtta ctttatttat tccactttca ctttttactg taacaaaagg acctccttgt
      181 tttagtactg tagcattttt tgaattaaca gtaaccgtta atcctttata ccggaataca
      241 ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa atgtaatttc atctttattt
      301 ggagaaaagc tactataaat aacttgacca tcttctgtaa cttgatggtt tttcaaaagc
      361 aattgatctt ttttatagcc tataggtaca taatcaaaat tctcaaattc ttcaagttta
      421 ttttccattt gttcattaga ataagttcct tgaaaattca tttctttcac catgttaact
      481 actgatgaat aatttaaaat tgaaatagct aatattaata atagtataaa atttcttctc
      541 cttgtataag aaaatttttc caactgtgga gaaataaata gagatactga aaaagatatg
      601 aacaatgttg aaaatatcat aaaacgccaa ggagcttgaa taatatttaa aggagtattt
      661 tgtaaggcaa accatggaaa tatttttgta caaagtagaa tagatattat acccattata
      721 taagtgattt tagataagat tcctaatttt ttaaagttaa atgttagtat aattaaacaa
      781 ataagcaaag ttaagccaat accaaatgat agtaattctg aatctatact tccaatcaaa
      841 aactctttac ccagataaat tgctcttttt tgtaatatat ccacataagg tctattcaga
      901 tcttgataca atgtctgctc aatcatagga cctataaacg ctagtgaaag aagtaaagta
      961 actatagcag cttttattaa tttaataatt aagaagattt ctattttctt tttccgataa
     1021 aattgaataa ttaaagcaat tataatcatt accatagtca tagctacaga aagaacatga
     1081 gtgtaaatta ataaagtcat gctaatcgcc aacataaccc aagattcttt ttttgagtcg
     1141 taaagcttta taaacccgag taaaataaga ggaagtattg acattgcgag taattccccc
     1201 attgcccctc gaagaaatat ttctacactc ctaaaagaag atgttgtata tataatagcg
     1261 aaaaaggagc tagtaacatg ttttttggta atttcataca ttacataatg acatataaat
     1321 agagtaatga gcgtaacaat ataaagaaaa aggttatagc ctattgctaa attagtaaac
     1381 ttgatcaata aaaataaagg atatagagtt aaccatgggt aaaaattatt tacttgcatg
     1441 ccatatgatc taaacgtttc aaaattaata ggggagctaa aaatagatt
//
LOCUS       DAYYCM010000176         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000176 DAYYCM010000000
VERSION     DAYYCM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.183"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHAY1_002957"
     CDS             64..972
                     /locus_tag="ACHAY1_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8594875.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHAY1_002958"
     CDS             972..>1480
                     /locus_tag="ACHAY1_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594876.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYCM010000177         1479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000177 DAYYCM010000000
VERSION     DAYYCM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.184"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAY1_002959"
     CDS             131..505
                     /locus_tag="ACHAY1_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8594877.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAY1_002960"
     CDS             complement(619..1248)
                     /locus_tag="ACHAY1_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8594878.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAY1_002961"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAY1_002961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          500 a          225 c          260 g          494 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataatagga
//
LOCUS       DAYYCM010000178         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000178 DAYYCM010000000
VERSION     DAYYCM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.185"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHAY1_002962"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHAY1_002962"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8594879.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta t
//
LOCUS       DAYYCM010000179         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000179 DAYYCM010000000
VERSION     DAYYCM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.186"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAY1_002963"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAY1_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8594880.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYCM010000180         1360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000180 DAYYCM010000000
VERSION     DAYYCM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.187"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1360
                     /locus_tag="ACHAY1_002964"
     CDS             82..>1360
                     /locus_tag="ACHAY1_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8594881.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          463 a          262 c          257 g          378 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DAYYCM010000181         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000181 DAYYCM010000000
VERSION     DAYYCM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.188"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHAY1_002965"
     rRNA            <1..1355
                     /locus_tag="ACHAY1_002965"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYCM010000182         1304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000182 DAYYCM010000000
VERSION     DAYYCM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.189"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHAY1_002966"
     CDS             290..1135
                     /locus_tag="ACHAY1_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8594882.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          491 a          156 c          213 g          444 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttg
//
LOCUS       DAYYCM010000183         1282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000183 DAYYCM010000000
VERSION     DAYYCM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.190"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHAY1_002967"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHAY1_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHAY1_002968"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHAY1_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          453 a          215 c          175 g          439 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac ta
//
LOCUS       DAYYCM010000184         1280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000184 DAYYCM010000000
VERSION     DAYYCM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.191"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..242
                     /locus_tag="ACHAY1_002969"
     CDS             <1..242
                     /locus_tag="ACHAY1_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8594883.1"
                     /translation="RQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLIL
                     DIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            247..1194
                     /locus_tag="ACHAY1_002970"
     CDS             247..1194
                     /locus_tag="ACHAY1_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8594884.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          419 a          185 c          267 g          409 t
ORIGIN      
        1 ctaggcaaca gacacctgaa gtttttgaaa tgaatgcctc aatgtattct tttaaacctg
       61 aatttctaaa aaaaagtaat tatatttttg atgggcaatg tgggataata aaaatgagag
      121 attatctaat tttagatatt gatagtgaag aagattttaa atggttagaa tttttgtttc
      181 ctaaattttt agaagattct tcggagcttc aagatatcta taattattta tatcggaggt
      241 aaaaggatga gctgttatct tatcaccggt ggggctggct tcattggttc taccttagcc
      301 aatcatttag gagaagaaca tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta
      361 gaaaatcttg atatgacgaa aaatatcact tttattgagg gagatgtcgc tgagaaatcc
      421 ttgatggaag agatcatgaa agctaataaa tttgattata tctttcattt agcagcagtt
      481 gctagtgtgg cagattcagt tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt
      541 gtattaatga ttttagaatt gatccgcaag tatcaacctg atttaaaacg gatcgtgttt
      601 agttcttcag cggctgttta tggggatgaa ccgactttac caaaaaaaga agagtccgtg
      661 attcgcccat tgacgcctta tgcggttgat aaattcgctg cagaacaata tgtgctagat
      721 tattgtcatt tatatgatgt acccggtagc gcagttcgtt ttttcaatgt ctatggtcca
      781 aatcaaaatc cgaattctcc atacagtggt gtgatttcta ttctcgtaga tcgctataag
      841 aaacaaatcg taggagaagc aacttcattt acattatttg gtgatggaag ccagtctaga
      901 gactttgttt atatagatga tgtaatccaa gcattacttt tagttgcgaa tgaggaagct
      961 gcactgggga aacaattcaa tgttggaacg ggtaaagcaa ccacactttt ggaattgatt
     1021 catgcaatca atgaaattct cggtgtagaa ttgacacttg aatacaaaga agagcgttcg
     1081 ggtgatatcc atgactctct agcagatatt actaagatcc agtcaattgg ctatcaaccg
     1141 aaatatggta ttttaagtgg aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata
     1201 ctaagataag attagagggt aaattcaggc tctttgtcaa taaggactga tgagttttgt
     1261 aaaatcaaat ctgggataga
//
LOCUS       DAYYCM010000185         1204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000185 DAYYCM010000000
VERSION     DAYYCM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.192"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHAY1_002971"
     CDS             <1..969
                     /locus_tag="ACHAY1_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8594885.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          218 c          243 g          340 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccg
//
LOCUS       DAYYCM010000186         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000186 DAYYCM010000000
VERSION     DAYYCM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.193"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHAY1_002972"
     CDS             <1..1076
                     /locus_tag="ACHAY1_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8594886.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAYYCM010000187         1168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000187 DAYYCM010000000
VERSION     DAYYCM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.195"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHAY1_002973"
     CDS             288..695
                     /locus_tag="ACHAY1_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594887.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHAY1_002974"
     CDS             709..1110
                     /locus_tag="ACHAY1_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594888.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          437 a          144 c          200 g          387 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaa
//
LOCUS       DAYYCM010000188         1106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000188 DAYYCM010000000
VERSION     DAYYCM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.196"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..800
                     /locus_tag="ACHAY1_002975"
     CDS             <1..800
                     /locus_tag="ACHAY1_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8594889.1"
                     /translation="GINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLL
                     QNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVV
                     GNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKF
                     LGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDF
                     VSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            833..>1106
                     /locus_tag="ACHAY1_002976"
     CDS             833..>1106
                     /locus_tag="ACHAY1_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8594890.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYV"
BASE COUNT          435 a          120 c          205 g          346 t
ORIGIN      
        1 ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc
       61 atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa
      121 ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa
      181 gtctgtttca aaaccacccc tttgagatag ttcataatgg tattgattta gagctttata
      241 gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt
      301 ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga
      361 tttttttaga aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt
      421 cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat
      481 ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc
      541 cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat
      601 tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat
      661 cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata
      721 aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta
      781 tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat ttttgaaaat
      841 atgttatttg atattggcat acaatgatat tggcaactta aagagattgg tcaatcgatt
      901 gaacgaaaat gcagttttta ttattcatat agataaaaaa actgatatag aaccatttat
      961 tgaagcattt aaatcatatg ataatgttga atttttatgt aataaaaacc gtgttaaaat
     1021 ttattggggt ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga gagcattaca
     1081 aaatgagaaa tatttaaaat atgtat
//
LOCUS       DAYYCM010000189         1080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000189 DAYYCM010000000
VERSION     DAYYCM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.197"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..697
                     /locus_tag="ACHAY1_002977"
     CDS             <1..697
                     /locus_tag="ACHAY1_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8594891.1"
                     /translation="ANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLAT
                     IPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLL
                     FGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYA
                     FYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYY
                     LGSISYLYLKKEN"
     gene            699..>1080
                     /locus_tag="ACHAY1_002978"
     CDS             699..>1080
                     /locus_tag="ACHAY1_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594892.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTY"
BASE COUNT          370 a          129 c          183 g          398 t
ORIGIN      
        1 tgccaattca aaatttagaa agagcattag agaagtacta cctgtaaaaa tcaaatggat
       61 ggtaagcata ttattagttc tattggtata tttttctttt ggtactaatt atgctaagga
      121 ttttttagca acaattcctg tatcgattga tagaaaagta gaagttatag agtcaaatga
      181 atcagataaa actccacttc cagaaaaaga agtaatatta cagcattcta gtgataactc
      241 cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg cttaaagtta taagaccaac
      301 tttgttattt ggttatggag agaccgattt ctatgggaat agcgatgcaa aatttttgaa
      361 aaaagtagat cttgatacta cagatgtgaa tgaactaaaa agagcacatg gaaacatgca
      421 taatggattt cttcaagtac ttgtatcaag tggtataatt gcttttatat gtatctatgc
      481 tttctatgtg ttaaatatct ttagtttact caaaacattt tttagaagta aagtgaatta
      541 tgcgtttctt ggtattgttt taatttttat actatctatt tttatagatg aaatggtgga
      601 ggctcatgta cttttcaata agcgcgatgt catttcaatt gttttctggt attatttggg
      661 aagcatttct tacttgtatt taaaaaagga gaattaatat gaagacagct ttcattgctt
      721 ggactccttt acatttgatt aatatattaa atgtttatca aaatttttac cgagaatcaa
      781 aaggagattt atttatttat tctgaatttg aaaacgctag aagatatagt gagcgtataa
      841 aagaattagg gctatttaat gatgttgttt ttgtgaatca ccggaaaatc ggttcttcct
      901 ttaaacgtaa aattaacttg ttaactaacc ataatcaatt tttctcggat gatttatccg
      961 tttatgatga aatttttata cagggggaaa attatttcac aaaaatactt ttctccaatg
     1021 tttacaaaaa aaatccagat gtaaaactaa attatataga agatggtttg ggtacttatt
//
LOCUS       DAYYCM010000190         1065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000190 DAYYCM010000000
VERSION     DAYYCM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.198"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>1065)
                     /locus_tag="ACHAY1_002979"
     CDS             complement(38..>1065)
                     /locus_tag="ACHAY1_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8594893.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          178 c          160 g          381 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caact
//
LOCUS       DAYYCM010000191         1037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000191 DAYYCM010000000
VERSION     DAYYCM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.199"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHAY1_002980"
     tRNA            complement(20..91)
                     /locus_tag="ACHAY1_002980"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHAY1_002981"
     tRNA            complement(113..200)
                     /locus_tag="ACHAY1_002981"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHAY1_002982"
     tRNA            complement(225..298)
                     /locus_tag="ACHAY1_002982"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHAY1_002983"
     tRNA            complement(312..382)
                     /locus_tag="ACHAY1_002983"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHAY1_002984"
     tRNA            complement(384..459)
                     /locus_tag="ACHAY1_002984"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHAY1_002985"
     tRNA            complement(478..551)
                     /locus_tag="ACHAY1_002985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHAY1_002986"
     tRNA            complement(564..653)
                     /locus_tag="ACHAY1_002986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHAY1_002987"
     tRNA            complement(677..750)
                     /locus_tag="ACHAY1_002987"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHAY1_002988"
     tRNA            complement(764..837)
                     /locus_tag="ACHAY1_002988"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHAY1_002989"
     tRNA            complement(865..937)
                     /locus_tag="ACHAY1_002989"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHAY1_002990"
     tRNA            complement(957..1030)
                     /locus_tag="ACHAY1_002990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAYYCM010000192         1021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000192 DAYYCM010000000
VERSION     DAYYCM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.200"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACHAY1_002991"
     CDS             2..460
                     /locus_tag="ACHAY1_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8594894.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACHAY1_002992"
     CDS             469..696
                     /locus_tag="ACHAY1_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594895.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DAYYCM010000193         1003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000193 DAYYCM010000000
VERSION     DAYYCM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.201"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACHAY1_002993"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACHAY1_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAYYCM010000194          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000194 DAYYCM010000000
VERSION     DAYYCM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.202"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHAY1_002994"
     CDS             <1..>986
                     /locus_tag="ACHAY1_002994"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8594896.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYCM010000195          963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000195 DAYYCM010000000
VERSION     DAYYCM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.204"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..783
                     /locus_tag="ACHAY1_002995"
     CDS             91..783
                     /locus_tag="ACHAY1_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8594897.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          422 a          147 c          161 g          233 t
ORIGIN      
        1 aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat
       61 gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt
      121 gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt
      181 ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat
      241 acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa
      301 cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta
      361 gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc
      421 aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac
      481 caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca
      541 caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca
      601 ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac
      661 gaagcaactg ctgaaaactc aagctggaaa caatatattc aaaagttaga aaaagcaaaa
      721 gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa
      781 taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactaagt
      841 agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt
      901 ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat
      961 tga
//
LOCUS       DAYYCM010000196          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000196 DAYYCM010000000
VERSION     DAYYCM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.206"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHAY1_002996"
     rRNA            <1..>943
                     /locus_tag="ACHAY1_002996"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYCM010000197          937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000197 DAYYCM010000000
VERSION     DAYYCM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.207"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..705
                     /locus_tag="ACHAY1_002997"
     CDS             13..705
                     /locus_tag="ACHAY1_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8594898.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          420 a          110 c          140 g          267 t
ORIGIN      
        1 gagaagataa aagtgagaaa aaagattatt attaatgctg gagtaattat aggattgatg
       61 ttagtaattt tctccggaaa gcaatatgct gataatattt tattatgggg aggggaagat
      121 aatattgaaa agatcaacaa caatttaact atattagaca cagctctaga aaataaagag
      181 caaaaaatta gtattttaaa ttcacaattg tcttcaaaca accaaactct tgaacaacta
      241 agaactaata ttgaagtttc caaacaaaaa gtggcatctt tagaaaacga caagaatcaa
      301 ttagtatcag agaaaactaa tttagaaaat caattgtcag caaaagactc tgaattgcaa
      361 ggaaaacaaa atgaaattaa tgcgaaaata gacgaaatta accaaaaagt agctgaaata
      421 aatcgattaa atagcgattg gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa
      481 gaacaatcta ctattaatag tttaaaccag caaattaatc aaatgttgag tgaaaaaaat
      541 aacattcaaa atcaactgca agctactcag caaaagctag aagaagaaaa gtcaaaaaat
      601 agtagtttga ataattacat tgaaaagctt caaaaagcta aaaatgatgt agaggatacc
      661 gcaaataaag cacaacaaat tgtaaatgaa catacaatcc aatagatata gttttaattt
      721 aacaaatggt atgtttggag ataatgagat agaatataaa tgtcataata ttatacaatc
      781 taaacaccgt ttccttttct atttttgatg acatcagtat atagctactc gaaagagtag
      841 tctttttatt attctcgctt attattgata tgaaaatttc gatttgacaa aaggtaccga
      901 ataattttgt aatatgaatt attatcaaga agtaatt
//
LOCUS       DAYYCM010000198          918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000198 DAYYCM010000000
VERSION     DAYYCM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.208"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>918
                     /locus_tag="ACHAY1_002998"
     CDS             <1..>918
                     /locus_tag="ACHAY1_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8594899.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECL"
BASE COUNT          278 a          185 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaa
//
LOCUS       DAYYCM010000199          904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000199 DAYYCM010000000
VERSION     DAYYCM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.209"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            768..>904
                     /locus_tag="ACHAY1_002999"
     CDS             768..>904
                     /locus_tag="ACHAY1_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8594900.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QL"
BASE COUNT          331 a          145 c          114 g          314 t
ORIGIN      
        1 tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga
       61 gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc
      121 attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt
      181 acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt
      241 ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc
      301 aaagacaata attattatga tccgttatta tctaagcact atacagataa taggaatttt
      361 tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta
      421 ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata
      481 cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc
      541 tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc
      601 ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac
      661 atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg
      721 cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt
      781 gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg
      841 tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac
      901 aact
//
LOCUS       DAYYCM010000200          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000200 DAYYCM010000000
VERSION     DAYYCM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.211"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHAY1_003000"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHAY1_003000"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAYYCM010000201          804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000201 DAYYCM010000000
VERSION     DAYYCM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.212"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(474..773)
                     /locus_tag="ACHAY1_003001"
     CDS             complement(474..773)
                     /locus_tag="ACHAY1_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8594901.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          280 a          126 c          114 g          284 t
ORIGIN      
        1 aagagtatta tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat
       61 gtatcctttt atggattaat gaaaggatgt ttcacatagt tatatttctg tatatttgaa
      121 aagttttact ttgattttta aatagaaaga catttgggtt atattgtgag ataataataa
      181 agaagagttt aaagcgttcc ccaaaaacca ctgccccata agtgtgttac gctttaaact
      241 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttacaatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
      361 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
      421 tcataagtat ggtgataaaa tccgttccgg gctacctttt taggtagcct actttaatct
      481 ttgtatcttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgctt
      541 cgagcgtcta gcacatcagt cacgatatca aacaaaaaat atacgtcttt cttcattcta
      601 gttttcgcag cagggatttt aaagtagttc ttattagaat agtagagatt aattaataag
      661 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      721 aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag cataaccatt
      781 actccgatct atttttaaac ttag
//
LOCUS       DAYYCM010000202          795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000202 DAYYCM010000000
VERSION     DAYYCM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.213"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>795
                     /locus_tag="ACHAY1_003002"
     CDS             <1..>795
                     /locus_tag="ACHAY1_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8594902.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          270 a          117 c          148 g          260 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttca
//
LOCUS       DAYYCM010000203          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000203 DAYYCM010000000
VERSION     DAYYCM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.214"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..379)
                     /locus_tag="ACHAY1_003003"
                     /pseudo
     CDS             complement(266..379)
                     /locus_tag="ACHAY1_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          269 a          113 c          123 g          228 t
ORIGIN      
        1 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
       61 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      121 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
      181 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
      241 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
      301 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
      361 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
      421 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
      481 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
      541 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
      601 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
      661 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
      721 ctatgcttta tat
//
LOCUS       DAYYCM010000204          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000204 DAYYCM010000000
VERSION     DAYYCM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.215"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHAY1_003004"
     CDS             complement(54..629)
                     /locus_tag="ACHAY1_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8594903.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYCM010000205          689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000205 DAYYCM010000000
VERSION     DAYYCM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.216"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..285)
                     /locus_tag="ACHAY1_003005"
     CDS             complement(<1..285)
                     /locus_tag="ACHAY1_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594904.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNST"
BASE COUNT          216 a          125 c          104 g          244 t
ORIGIN      
        1 agttgaattt tttaaatcta taagagtaaa accaattcct tcatactgaa tttctgaaaa
       61 tcgaattttt ctcccagttg atagatcaag gggcagaaaa ccttttatta cattgtgagt
      121 gtcaccatca tttacaatgt cattaggaat tttgatttta gaattctgtg gagcacctat
      181 tggaagataa taattcatta taattttgta gtcttcatga ggaaataaag tacttttagg
      241 attgctaaga tattctcgta ccgaatctaa gaatggctta tccattgaat cttcatcccc
      301 atttaaacta aaaaacattc ctacaattgc ttttgcaact ctaagtgaat tcattttctt
      361 tattgaatgt gttaaatcag acttaccatt ctttaaactt gtattgttat aaaggaaatt
      421 tttattgtct tctgcaaatc taatatattc ctcaacatac tcactaccaa aaaaactatt
      481 acatttacta cataacgtat tggaatggca acagtttttt tgacaaattt tataaggtgc
      541 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
      601 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
      661 gctcattatc aaggattcgc tgcgccaat
//
LOCUS       DAYYCM010000206          660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000206 DAYYCM010000000
VERSION     DAYYCM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.217"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>660
                     /locus_tag="ACHAY1_003006"
     CDS             19..>660
                     /locus_tag="ACHAY1_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594905.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYS"
BASE COUNT          274 a          103 c          103 g          180 t
ORIGIN      
        1 ataaaaagga gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa
       61 ttacgccttt catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca
      121 cgtgcgacca tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa
      181 ttattatcaa ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa
      241 tatataactt cataccttat cgatataggg tatgagctat atattaagga cttcccagaa
      301 atacctcaca aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat
      361 aaagactatg agttattgaa tcctataata aaaaatatat ttacaaaata ttactcaaaa
      421 gattttgaaa tttatctaaa tgagatactt gctaataata tcaatcctct gattgaagag
      481 tacgctaacc aaagacactc tatctctaaa gagctatttt tcaaaagagc caacagtaaa
      541 cttcaagaca agataagaaa tggtgatttc aaatacggtg aaaaagaaag aatgattgat
      601 tctttaaaac agctattaga cgaaatggac ttagcctata aagatatttc aaagtattca
//
LOCUS       DAYYCM010000207          657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000207 DAYYCM010000000
VERSION     DAYYCM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.218"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            319..618
                     /locus_tag="ACHAY1_003007"
     CDS             319..618
                     /locus_tag="ACHAY1_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8594906.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          252 a           83 c          121 g          201 t
ORIGIN      
        1 taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga
       61 taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta
      121 ggcgtaattc taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt
      181 ttaaaataaa aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat
      241 ttaaaaaaat ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat
      301 ttttgggagg aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta
      361 tatgatctcg ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca
      421 aagacaggct tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt
      481 ttacgtccgt tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag
      541 atatcaacaa taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt
      601 acttttgggc atttataaaa acgagttatt catctcaatg aattaaaaag tatagtc
//
LOCUS       DAYYCM010000208          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000208 DAYYCM010000000
VERSION     DAYYCM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.219"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACHAY1_003008"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHAY1_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAYYCM010000209          605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000209 DAYYCM010000000
VERSION     DAYYCM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.220"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>605
                     /locus_tag="ACHAY1_003009"
     CDS             <1..>605
                     /locus_tag="ACHAY1_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594907.1"
                     /translation="LHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQ
                     NAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYT
                     YNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEE
                     IGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGT"
BASE COUNT          247 a           63 c          110 g          185 t
ORIGIN      
        1 tattacatgg aagagttatt aacttagtta atcgaaaaag tacgactaaa tttgcaaact
       61 cattctggtt tttaaaaaat attaataaaa cagtggaaaa gaatggagtt tataaaggtg
      121 attttcattt tcaaaatgct gagactattg aacgagaaca tcattatttc gatttattag
      181 tgaatacagt ggtagaatta gcgaaatggg caaagtcaaa cgaaacttat agtcaagtac
      241 ttaaaagaat tatgagtcaa gaaacttcat ttatctatga gttaagcaag tcaaatttga
      301 aatatactta taatgaagat gaacttgaaa ttttagataa tccagaaaat tcacaatgga
      361 ttaaagagtt gaagagagaa gactttattg agacactaag aattctaaaa tcgttatttg
      421 atttgaaatt agccaaagct accatcatgg gaattgaaag agattcatat tattgggagg
      481 aaattggact atatgctccc cagattgaag gtgaagataa agagaaaagg atagacaaca
      541 ttattaacta tattaatagt ttaagtgaac ctttaaaatg tagaggactt gattttatag
      601 gaaca
//
LOCUS       DAYYCM010000210          598 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000210 DAYYCM010000000
VERSION     DAYYCM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.221"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..172
                     /locus_tag="ACHAY1_003010"
     CDS             <1..172
                     /locus_tag="ACHAY1_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8594908.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            173..>598
                     /locus_tag="ACHAY1_003011"
     CDS             173..>598
                     /locus_tag="ACHAY1_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8594909.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
BASE COUNT          234 a           68 c          111 g          185 t
ORIGIN      
        1 aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga
       61 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
      121 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
      181 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
      241 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
      301 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
      361 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
      421 tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa
      481 tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct
      541 tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagtt
//
LOCUS       DAYYCM010000211          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000211 DAYYCM010000000
VERSION     DAYYCM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.222"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHAY1_003012"
     tRNA            complement(65..150)
                     /locus_tag="ACHAY1_003012"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHAY1_003013"
     tRNA            complement(167..238)
                     /locus_tag="ACHAY1_003013"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHAY1_003014"
     tRNA            complement(250..322)
                     /locus_tag="ACHAY1_003014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHAY1_003015"
     tRNA            complement(340..421)
                     /locus_tag="ACHAY1_003015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHAY1_003016"
     tRNA            complement(429..501)
                     /locus_tag="ACHAY1_003016"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHAY1_003017"
     tRNA            complement(505..577)
                     /locus_tag="ACHAY1_003017"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYCM010000212          576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000212 DAYYCM010000000
VERSION     DAYYCM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.223"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..304
                     /locus_tag="ACHAY1_003018"
     CDS             5..304
                     /locus_tag="ACHAY1_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8594910.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(340..>576)
                     /locus_tag="ACHAY1_003019"
     CDS             complement(340..>576)
                     /locus_tag="ACHAY1_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594911.1"
                     /translation="LKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRML
                     EKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          181 a           96 c           85 g          214 t
ORIGIN      
        1 ggttatgctt tttaatgaga cacaattatg gtttaaattt gacccttcga atagatttat
       61 caaagatttt tataaagtat ggaattcaga agttttcttt ttagcaatcg aagatagctt
      121 attaatcaat ctctactatt ctaataagaa ctatttcaaa attcctgctg cgaaaactag
      181 aatgaagaag ggtgtatact ttttgtttga tatcgtgact gacgtgccgg acgcacgtag
      241 cgatcatcgg cgttatgact atataaagta tactttcgtt gatccagaaa gatacaaaga
      301 ttaaataaaa aaataacaaa caaactcgct tgaaattcat taggtgagtt tgtttattat
      361 tttttctgct tctcgattca tcagcgccat atctgttttc gtaatggttt cattaggaat
      421 atagtcagca cattttcttt tctctaacat tctataccca ctattatgta ttcctaaggt
      481 attcaattcc ttaactgttg aaaacaaatt ccaaacggtt tggtggcttc cttttccgct
      541 acttccatta taaataatat cataattttc tttcaa
//
LOCUS       DAYYCM010000213          550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000213 DAYYCM010000000
VERSION     DAYYCM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.224"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAY1_003020"
     CDS             complement(132..509)
                     /locus_tag="ACHAY1_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594912.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          163 a           99 c          100 g          188 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt
//
LOCUS       DAYYCM010000214          469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000214 DAYYCM010000000
VERSION     DAYYCM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.228"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>469
                     /locus_tag="ACHAY1_003021"
     CDS             <1..>469
                     /locus_tag="ACHAY1_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8594913.1"
                     /translation="ACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNL
                     AYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSY
                     FYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           84 g          144 t
ORIGIN      
        1 gcttgtgtta tatggggcat aattaatggc tatactagcc aaagagccac gtatagagcg
       61 gtttgttccg tcttatttcc taatggtgac tttcctagta ggagtcgatt cactcgctta
      121 agttcaaact tagcttacac tatcaagatt attcgatact ttttcatcaa gaagctcacc
      181 aaaggtgaac tagtcggaat tatagatagt tttcctagtc cattatgtaa accagttaga
      241 aatagacaag caaaactatt gaatcaaata gccaaagttg gctataactc aacaaaaaaa
      301 tcttattttt atggtcttaa aattcatatg atcgttacta agactggctt tccgattact
      361 tactcaatca cgaatccagg tgtccatgac gtgaaagtgt tagagacttt atcggaagaa
      421 gcgaatcttc ccaatatcct aggggacaaa ggatatatta gccataaaa
//
LOCUS       DAYYCM010000215          461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000215 DAYYCM010000000
VERSION     DAYYCM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.229"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>461
                     /locus_tag="ACHAY1_003022"
     CDS             <1..>461
                     /locus_tag="ACHAY1_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8594914.1"
                     /translation="GTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQ
                     PITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKY
                     TWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          187 a           68 c          108 g           98 t
ORIGIN      
        1 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca
       61 gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag
      121 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
      181 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      241 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      301 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      361 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      421 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAYYCM010000216          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000216 DAYYCM010000000
VERSION     DAYYCM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.231"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHAY1_003023"
     CDS             <1..>451
                     /locus_tag="ACHAY1_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594915.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYCM010000217          437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000217 DAYYCM010000000
VERSION     DAYYCM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.232"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           75 c           60 g          176 t
ORIGIN      
        1 gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa
       61 cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg
      121 agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat
      181 catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat
      241 ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga
      301 tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct
      361 cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata atttaatttt
      421 tttgttccta tttattt
//
LOCUS       DAYYCM010000218          421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000218 DAYYCM010000000
VERSION     DAYYCM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.233"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAY1_003024"
     CDS             complement(<1..132)
                     /locus_tag="ACHAY1_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8594916.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          143 a           82 c           83 g          113 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 g
//
LOCUS       DAYYCM010000219          408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000219 DAYYCM010000000
VERSION     DAYYCM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.234"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHAY1_003025"
     CDS             <1..387
                     /locus_tag="ACHAY1_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594917.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           74 c           65 g          113 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggac
//
LOCUS       DAYYCM010000220          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000220 DAYYCM010000000
VERSION     DAYYCM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.236"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHAY1_003026"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHAY1_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYCM010000221          303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000221 DAYYCM010000000
VERSION     DAYYCM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.238"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAY1_003027"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAY1_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8594918.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          113 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atg
//
LOCUS       DAYYCM010000222          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000222 DAYYCM010000000
VERSION     DAYYCM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.240"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHAY1_003028"
     CDS             <1..>282
                     /locus_tag="ACHAY1_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8594919.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYCM010000223          268 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000223 DAYYCM010000000
VERSION     DAYYCM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.242"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>268)
                     /locus_tag="ACHAY1_003029"
     CDS             complement(36..>268)
                     /locus_tag="ACHAY1_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8594920.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           79 a           39 c           47 g          103 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaata
//
LOCUS       DAYYCM010000224          256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000224 DAYYCM010000000
VERSION     DAYYCM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.243"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           61 c           37 g           79 t
ORIGIN      
        1 cttgtcaaag gggcgcaagc tcccccttga atccccctca aaacccacgc tccacaatac
       61 aaaaaaggct agcaaatgct agccactttt tgtaaagttt cgcttgtttc accttcggtg
      121 aaattattgt tttgtaatat caaacaacta ttcatcaatc agctttaaaa aattcaattg
      181 gtttagctgt cccttctgtc ccatcattcg ttgtcaaaat aaatacgagc ttttttggag
      241 aatccaaact aaaagt
//
LOCUS       DAYYCM010000225          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000225 DAYYCM010000000
VERSION     DAYYCM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.244"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHAY1_003030"
     CDS             <1..>253
                     /locus_tag="ACHAY1_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8594921.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYCM010000226          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000226 DAYYCM010000000
VERSION     DAYYCM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.denovo.246"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACHAY1_003031"
     CDS             <1..>247
                     /locus_tag="ACHAY1_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8594922.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAYYCM010000227        35533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.017, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000227 DAYYCM010000000
VERSION     DAYYCM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.017"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..389
                     /locus_tag="ACHAY1_003032"
     CDS             75..389
                     /locus_tag="ACHAY1_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594923.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(442..1215)
                     /locus_tag="ACHAY1_003033"
     CDS             complement(442..1215)
                     /locus_tag="ACHAY1_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594924.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1320..1565
                     /locus_tag="ACHAY1_003034"
     CDS             1320..1565
                     /locus_tag="ACHAY1_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8594925.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1721..2572
                     /locus_tag="ACHAY1_003035"
     CDS             1721..2572
                     /locus_tag="ACHAY1_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8594926.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2605..3282
                     /locus_tag="ACHAY1_003036"
     CDS             2605..3282
                     /locus_tag="ACHAY1_003036"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8594927.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3770..4735
                     /locus_tag="ACHAY1_003037"
     CDS             3770..4735
                     /locus_tag="ACHAY1_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8594928.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4757..4969
                     /locus_tag="ACHAY1_003038"
     CDS             4757..4969
                     /locus_tag="ACHAY1_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8594929.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5044..5520
                     /locus_tag="ACHAY1_003039"
     CDS             5044..5520
                     /locus_tag="ACHAY1_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8594930.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5517..7325
                     /locus_tag="ACHAY1_003040"
     CDS             5517..7325
                     /locus_tag="ACHAY1_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8594931.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7402..7617
                     /locus_tag="ACHAY1_003041"
     CDS             7402..7617
                     /locus_tag="ACHAY1_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8594932.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7709..8572
                     /locus_tag="ACHAY1_003042"
     CDS             7709..8572
                     /locus_tag="ACHAY1_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8594933.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8609..9145
                     /locus_tag="ACHAY1_003043"
     CDS             8609..9145
                     /locus_tag="ACHAY1_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8594934.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            9159..9581
                     /locus_tag="ACHAY1_003044"
     CDS             9159..9581
                     /locus_tag="ACHAY1_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8594935.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9511..11910
                     /locus_tag="ACHAY1_003045"
     CDS             9511..11910
                     /locus_tag="ACHAY1_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8594936.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11942..13933
                     /locus_tag="ACHAY1_003046"
     CDS             11942..13933
                     /locus_tag="ACHAY1_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8594937.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13956..14207
                     /locus_tag="ACHAY1_003047"
     CDS             13956..14207
                     /locus_tag="ACHAY1_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8594938.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14197..15426
                     /locus_tag="ACHAY1_003048"
     CDS             14197..15426
                     /locus_tag="ACHAY1_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8594939.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15423..17504
                     /locus_tag="ACHAY1_003049"
     CDS             15423..17504
                     /locus_tag="ACHAY1_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8594940.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17653..21573
                     /locus_tag="ACHAY1_003050"
     CDS             17653..21573
                     /locus_tag="ACHAY1_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8594941.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21574..22518
                     /locus_tag="ACHAY1_003051"
     CDS             21574..22518
                     /locus_tag="ACHAY1_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594942.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22522..22725
                     /locus_tag="ACHAY1_003052"
     CDS             22522..22725
                     /locus_tag="ACHAY1_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8594943.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23025..23261)
                     /locus_tag="ACHAY1_003053"
     CDS             complement(23025..23261)
                     /locus_tag="ACHAY1_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594944.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23444..23815)
                     /locus_tag="ACHAY1_003054"
     CDS             complement(23444..23815)
                     /locus_tag="ACHAY1_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8594945.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23812..25140)
                     /locus_tag="ACHAY1_003055"
     CDS             complement(23812..25140)
                     /locus_tag="ACHAY1_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8594946.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25101..25430)
                     /gene="mobC"
                     /locus_tag="ACHAY1_003056"
     CDS             complement(25101..25430)
                     /gene="mobC"
                     /locus_tag="ACHAY1_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8594947.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25688..26058)
                     /locus_tag="ACHAY1_003057"
                     /pseudo
     CDS             complement(25688..26058)
                     /locus_tag="ACHAY1_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            26203..26388
                     /locus_tag="ACHAY1_003058"
     CDS             26203..26388
                     /locus_tag="ACHAY1_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8594948.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26546..26713
                     /locus_tag="ACHAY1_003059"
     CDS             26546..26713
                     /locus_tag="ACHAY1_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594949.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27331..27990
                     /gene="vanR-B"
                     /locus_tag="ACHAY1_003060"
     CDS             27331..27990
                     /gene="vanR-B"
                     /locus_tag="ACHAY1_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8594950.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27993..29354
                     /gene="vanS-B"
                     /locus_tag="ACHAY1_003061"
     CDS             27993..29354
                     /gene="vanS-B"
                     /locus_tag="ACHAY1_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8594951.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(29445..30746)
                     /locus_tag="ACHAY1_003062"
     CDS             complement(29445..30746)
                     /locus_tag="ACHAY1_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8594952.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            31025..31831
                     /locus_tag="ACHAY1_003063"
     CDS             31025..31831
                     /locus_tag="ACHAY1_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8594953.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            31849..32556
                     /locus_tag="ACHAY1_003064"
     CDS             31849..32556
                     /locus_tag="ACHAY1_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8594954.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            32553..33524
                     /locus_tag="ACHAY1_003065"
     CDS             32553..33524
                     /locus_tag="ACHAY1_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8594955.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            33517..34545
                     /gene="vanB"
                     /locus_tag="ACHAY1_003066"
     CDS             33517..34545
                     /gene="vanB"
                     /locus_tag="ACHAY1_003066"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8594956.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            34551..35159
                     /locus_tag="ACHAY1_003067"
     CDS             34551..35159
                     /locus_tag="ACHAY1_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8594957.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(35225..35398)
                     /locus_tag="ACHAY1_003068"
     CDS             complement(35225..35398)
                     /locus_tag="ACHAY1_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594958.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9386 a         8652 c         9260 g         8235 t
ORIGIN      
        1 tgaaaaatga tagagaaaat ctgatacgtc cgcaacaatg gacaattgaa aatgaaagtg
       61 aggaaattgg aagaatggct gatgaaaata aagttgttcc gcttgaaaca ctagtgataa
      121 ataaggaaga gtttgagcaa ctttttcttg atttttttgt tcaatatgac aggatttatg
      181 attttagttc gtcactagat ttatttgaat tagaggcaga aataatagaa aacagtcgag
      241 aggagaatgg taaaatcatc atttcagtag aaaaattcaa tgaattattc tcagaaacat
      301 ttgaaataga tacagattat tatcaaccac aagaagtttt ccaatcattt agaaatcaga
      361 ttattcgctc aggtaagacc gtaaactgaa aaacaataaa agaaacgttg aaagaataat
      421 tattccttca acgtttcttt tttagaatgt tttaatgacc aaagcagttt cattgtcagg
      481 cttaggtaat tcatgaaatc ccgtatttat agtgctcaat tgatcaacta accatgtttt
      541 actttttgaa ttgtagataa atctaaaatt aatatcgtta ttatcgtgtt gaaggggcgt
      601 ctccaaataa tctgctgtat attcatagtc aacgtaacct gctaatacaa aagagagtat
      661 ccctttattt aatgttagat ttaatggata atcatacgac tttatttttt tcaacttcat
      721 attgtacata tcttcagcca taaaagttga gttcgtgatt tcatcttggg tctcattaaa
      781 aaagttatcc gaataatcgc ttatttttga aaagtcttta ttggcaccag cctcaaaaaa
      841 ttgttcaact ttttcagtat aaagttgtgc taacgctcct ttttcaatat taactttaat
      901 attttcatgt ctatcaattt gtgtgctgtt cagtaaggga agatcagctt ttagttttat
      961 atcgtcttga tttctttcaa tttcaattgg caaaatcttt tcagtagatt cagtttctga
     1021 taaaggaaca tctagttttt tatcatgata ataagcttca aatccatctg gaaccggaaa
     1081 aaaatgtatt gggtaattct tttctgaggt tgttgtacta gtttccgtac ttgtagaact
     1141 tgtgtcagaa gtggtacacc cgcttatagt tccgatgagt aaagcgcaag ttagaatgat
     1201 tgcaaatttc ttcaaaaaaa ttcctcctta aaattgaata tggactaata ctagtcccat
     1261 tgtagcatac cgaatcttta agattaataa gaaaaaggac taattttagt ccaaaaggtg
     1321 tgctattctt gaaagaggaa aaaattattg ataaagaaaa tgtaatcggt agcaatatta
     1381 gacgagtccg attagaaaaa ggaattggtc aaaccgagct gattagagac ctacagcttc
     1441 gtaaaattgc tataactagg gaaacgttgg ttaagattga aggtggccgc caacacatta
     1501 agttggatca attgaaagcc atcaaagata ttctacaggt gtcttatgaa gatttgcttc
     1561 aatgactact tgtagtcatt tcgtttgaaa ttattatgtt acacccttat gtgtcacgaa
     1621 gaaatactgg tataataatg aatgggatat gtaacgatag ggtatactgt tatgttgcct
     1681 aataaattgt ggatcaaaat acaagaatgg agggatatcc ttgtataagg catatgaatt
     1741 ggattttaca aatgctgact taaatgcatt ctcctcaagc tcgcaatcat atacacatgt
     1801 agtaaatcaa atagatcaca accaaaaatc tattaacaag agaatagaaa atctatttga
     1861 aagtgaaaat gatttagttg attattattc taaggacagc aaaattctag atgcagacga
     1921 aattattaaa gattggtttc caactataaa agcagatatt tttatatctc actctcatgc
     1981 tgatgaaaaa ttagctatac gttttgcatc atggctattt gaaaattttg gtcttactgc
     2041 atttattgat tcttcggttt ggggatacag ttctgattta ttaaaaaaaa tagaccaaaa
     2101 atattgctat aaggaacaaa caaaaacata tgattatgat aaaagaaatg taacaactag
     2161 tcatgttcat atgatgcttt ctacagcttt aaataatatg attgatagta cagaatgtct
     2221 gttttttctt aatacaccta attcaattag tttgagcaat gaaatcacta atgaacagaa
     2281 atttacgtat tcgccatggc tatatagcga actgacgaca gcttcaatag tggaaaaaaa
     2341 gaatccacga ttggaaagta atcctcaaat gagcaccgag gatgttagaa gcattataaa
     2401 gcactattct tcgcaagaaa atctgcaaat aaaatattct ttaaatttaa gtgaattaac
     2461 ttatttacga aaagctgatt tgaaaaaatg ggctttagat gctgtggaat gcactaaagg
     2521 aagtaataca ttagatattt tatataaaaa caccattttc actaatctct aatcaattta
     2581 ctgaatcgaa aggaaccggg tagaatggat aagaaagttt taataaagag gttctctgaa
     2641 gctattgaaa aaggaaatgc agcaatattt gcaggtgcag gattatcgat gtcacaagga
     2701 tatgtaagtt ggccggaact acttaatgat cctgcaacag aaatcgggtt ggattctaaa
     2761 aaagaaacag acttagttac tctggctcaa tattataaaa atgaaaatgg tggatcaaga
     2821 ggaattttga atcagatact tatggataat tttggtgaag aattagaaat atctgaaaat
     2881 catcgaatac tagcaagttt acctattgaa acttattgga caactaatta tgatcatcta
     2941 atagaaaaat caatccgaga agcttataaa aaccctcagg ttaagaaaaa ttatactcag
     3001 ttagcaacaa ctaatccaaa tgtggataca attgtttata aaatgcatgg tgatatagat
     3061 gatgtcagtt cgacagtaat aacaagagat gattatgaaa aatatgatga ttcgtacgcg
     3121 ttatttaaag aaactttaaa aggagatttg ttaacaaaaa cttttctctt tttaggattt
     3181 agttttacag accctaattt agaaagaatt ttatcagata taagatgggt tttgagagaa
     3241 aaccaaagac ctcattattg tataatgaga aaaatattat agaaaaaatt ttaggttatc
     3301 gctgaaagcc ccggaaatac gggcatttcg caggatatga gaactcaatt tactaccaat
     3361 ttactacttt cggattgagc cgcatatatg ctgaaccata ctgctaatac ccgccacccc
     3421 aaatacggca catcataaat tgaatacgac accgtaaacg cggcgttcct ctttcccgtc
     3481 atgggagaac aggacgccgc ttttttatgc ccaaatagag aaaggaggcg acgccatgct
     3541 atttacttcc ggccctgacc gggcgtttga acggctgatg caggaaaagc ccggtttcga
     3601 tcactacgac ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc gattttaccg
     3661 cccggattgg aaatatcaat tctgccagtt tgcagagtgt ccctatcagc cgggcaagct
     3721 gaccgactat gcagcggtca aattcacagt gaaaggagcg aacaacgcta tggcagtttt
     3781 ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat caccacctgc ggaataagaa
     3841 cctgacctta aaggccaagg gcctgctgtc gcagatgttg tccttgccgg aggattggga
     3901 ctatacccta aaaggactgt cctatatcaa ccgggaaagc atcgacgcga tcaggacagc
     3961 ggtatgggag cttgaaaaag ctggatatat ccggcgtgag cagggccgtg acgcaaaagg
     4021 caaaatggcc gatatggtct ataccatcta cgaacagccg gtattggaaa acccggtatt
     4081 ggaaaatcca acatcggata acccggtgtt ggaaaatccg acatcggata atccggcgtc
     4141 ggaaaatcca acgcaattaa ataaagaagt acaaagaact gacttatcaa aaaaagaaaa
     4201 aataattaca gatggacaaa gtacccattc cattcctatc ccatccccga cccctgcccc
     4261 tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa aggacaggaa aggatgaggc
     4321 tgtacggatt tatcgggaaa tcattttgga gaacatcgag tatgcctatc tcattcagga
     4381 caggagcatt gaccgtgaac agcttgacga aatcgtagac ctgatgctgg aaaccgtctg
     4441 caccgcccga aagacaatcc gtgtcgccgg ggacgactac cctgccgagc tggtgaaatc
     4501 caagtacatg aagctgaccg gcgaacatat ccgctttgtc cttgactgtc tgcgggagaa
     4561 cacaacgaaa gtccgcaata tcaaacaata cctgcgggct atgctgttca acgcccccag
     4621 caccatttcc aactactaca cggcccttgt cgcgcacgac atggcacagc ccgattgggg
     4681 aaagcctaaa tccggcctgc cggactattc ctgcggcgag ggcgaaagcc tgtaacgaga
     4741 aaaggaggac gaccctatga agcagggcgc attgattttt gacgagcgaa cagaccgtta
     4801 cgacatccgc tttgatctgg ccgactatta cggcggcctg cattgcgggg agtgtttcga
     4861 cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa atggccgaaa actggtatct
     4921 tgtcggcatc cgggccgacg acctttccgg cctgcgggtg cggatttgac ctatatctac
     4981 ctgcggcgtc tggtggttcc gccttagacg ccgcttttga ctttctgaca aggagggatg
     5041 cgattgcagg aagaagttac tcaaaagacc attgccctgt ccatgaaaac gggcaagctc
     5101 acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc aacaccgggc caagggcaaa
     5161 aacacgctgc accacggcca gcagagccta aagcagctta aaaagcatgg ggctgatctc
     5221 tccaacattg agatcaccga ggccaacatt ggagcgttca agccatgcgc caagaaatac
     5281 ggcgtggatt ttaccttacg aaaagataag accacccagc cgccccacta cgttgtgatt
     5341 ttcaaggcca aggatgcaga taaccttgat ctggcgtttc gggagtttac cgccaagacg
     5401 ctttccaagg agcagcggcc ctctatccgc aaggttttgt ccgctgtgaa gcaaaaggcg
     5461 gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg aaagggggct ggaactgtga
     5521 agcctgacgt aaagaaattg ctgatcctga acctcccgta tctgattttt gtgtacctgt
     5581 ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc tgacctgtcg gggaagctcc
     5641 tgcattttgc ggatggtttt gcggcggctt ttgccaaccc tctgccaagc ctgcacctgt
     5701 ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg gctggtggtg tactgcaaga
     5761 gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc tgggggacgg
     5821 ccaaagacat agcgccttac atcgacccca agtttgaaaa taatatcctg ctcacccaaa
     5881 cggaacggct taccatgacc ggcaggccca aagaccccaa gacagcgcgg aataaaaacg
     5941 tgctggtgat cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa cccaacctga
     6001 tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat ccgaaaggca
     6061 ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt aaaatcctga
     6121 ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc cattcggaaa
     6181 aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag ggggaaaagt
     6241 cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg atcggctata
     6301 tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa atgataaacg
     6361 ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc atgtttgagc
     6421 gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt
     6481 ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt gcgccctttg
     6541 acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac accatcgggg
     6601 acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt aatttcattc
     6661 tggctatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg
     6721 gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac attggccaaa
     6781 tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg gcctctatta
     6841 ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag atcatttcgg
     6901 ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag gagatttccg
     6961 atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg ggaagccaaa
     7021 cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa gacgaaattg
     7081 ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg
     7141 agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc gacaagaaga
     7201 atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag caagtggtaa
     7261 cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg
     7321 aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat gagccggaaa
     7381 ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac tttgcagact
     7441 atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct gttggaggga
     7501 tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcagcttgt cgctggcggc
     7561 ggcgtggccc tgatcggtgt cactctggtt cccctgctgt ccgggctgct gggctgatcc
     7621 tggcctttgt ccctgtccaa acccttaact aatcgcctcc gcgtcctccc ggccagaccg
     7681 ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa atcattgaag aatggataaa
     7741 aggcatcctc atggacggta tcacgggcaa cctgtccggg ctgttcgata cggtaaatgc
     7801 caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg caggcatgga acagcggtat
     7861 tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg gggccatact
     7921 cgcccttgtc atgtgctatg agcttatcca gatgataacg gaaaagaaca atatgcacga
     7981 ctttgacagc tccatgttct tccgctggat attcaaatcc gcgtttgcaa tcctgatcgt
     8041 aacgaacaca tggaacatcg tcatgggcgt attcgacgcc acccaaagcg tcgtcaatca
     8101 aagcgccggt gtgattatcg ggcagacatc cattgatttt aacaccctga taccagacct
     8161 tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg gggctgtggt tccaaaccct
     8221 tgttgtgggc ctcaccatga atatcctttc catctgcatt ttccttgtga cctatggccg
     8281 catggtggaa atctatgttg tcacgtcgct ggggcctatc ccccttgcca caatgggcaa
     8341 cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc tgggcttcca
     8401 agccttttta atcatggttg tggtgggtat ctacgcggtg ctgatacaga atatcggttc
     8461 cgcttccgac atatccggcg caatctgggg ctgtatgggc tatacggtgc tgctgtgctt
     8521 ctgcctgttc aagaccggca gtatatcaaa ggccgtattc ggggcgcatt gatcccaaaa
     8581 tccaagcata cacaggagga acttgtttat ttcaacgtac aacatcattt acgctgaccc
     8641 gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg gaaaatcact attccaccat
     8701 gagcattgac gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag actgcctgct
     8761 gtttttgtgg gccacattcc ccatgctgcc ggaagctctg cggttaatca aagcgtgggg
     8821 cttttcattc aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa gccttacatg
     8881 gttttacggg ctgggccgct ggacaagggg aaacgcggaa atctgcctgc tggcaacaca
     8941 cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc cgattgaaca
     9001 acacagcaaa aagccggatg tgacccgtga aaaaatcgta gagctggcgg gcgatttgcc
     9061 ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg ggaacgaggt
     9121 ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat ggcttatgta accgtcccca
     9181 aggacttaac ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg caactgatct
     9241 gtttttccgg ggcgctggtg atcggcgtcc ccttatattt tctcacccgt gactatctta
     9301 ccaacagcgc ggcggccctg ctcatggtat ttgccatgct gccgggcttt ttgcttgcca
     9361 tgtacgagcg tcacggccag ccccttgaag tggtgattat gcagatcatc cagtgctgct
     9421 tcatccagcc aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc cttgtgcggc
     9481 aataccagat ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc aagagaaacc
     9541 gaaaaaacaa agctgacccg cgcccagcga aaagaaattg acgcggtaat ccgtaaatat
     9601 aagggcgacg gcaggcccca cacggcgcag cagagcatcc cctacgaggt catgtacccg
     9661 gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt gcattgagtt tgcggacatc
     9721 agctatcagc ttgcccagcc ggacacgcag acggctatct ttgaaaaact gtgcgacctg
     9781 tataactatg tggacgcctc cattcatatc cagttttctt tcctcaatcg gaaagttgac
     9841 ccggtgcagt acgccaagag ctttgagatc gcgccgcagg gggacgactt cgacgacatc
     9901 cgggccgagt acaccggcat tctgcaaaag cagcttgcaa acggcaacaa cggcatggta
     9961 aagaccaaat acctgacatt caccatcgag gccgaaagcg tgaaagcggc gcgagccaga
    10021 ttgaagcgca tcggctttga cctgttaggc tatttcaaga gcatgggggc cgtagcccat
    10081 gtgatggacg gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc ggacggcgag
    10141 atattcaatt ttgactggaa gtggctggcc ccgtccggcc tctccaccaa ggattttatt
    10201 gccccgtcct ccctgtgctt cggcaatgca aagacattcg gcatgggcgg caaatacggc
    10261 gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg acgatatgct ggccgacttc
    10321 ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc aggccattga acagaccaag
    10381 gcgatcaaga ccatcaagcg caaaattacc gaccttgacg ctatgaagat tgcggagcag
    10441 aaaaaggccg tccgcagcgg ttacgatatg gacattcttc ccagcgacct tgccacctac
    10501 ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg gctgttccag
    10561 ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa agctgaataa cgacgtgttc
    10621 caagccgcag gcgtggccca aaagcataac tgtccccttg tccggctgga ctaccagcag
    10681 gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat acagcgcagc
    10741 cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct ttttcagggc
    10801 ggcgcggcca tgtattacgg catcaacgcc aagtcccgca acatgattat gctggacagg
    10861 aagcaggccc gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg caaatccatg
    10921 agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat ttttatttca
    10981 gacccggagg ccgaatatta cccccttgtc aaacggctcc acggacaggt aatccggctg
    11041 tcgcctacca gcaaggactt tgtgaacccg ctggacatca acctgaatta ttcggaggac
    11101 gataacccgc tggccctgaa atccgacttt gtgctgtcgt tctgtgagct tgtcatgggc
    11161 ggcaagaacg ggctggaagc cattgaaaag accgtgatag accgggctgt gcgcgtgatc
    11221 taccggccct atctggcaga cccccgcccg gagaatatgc cgatcctgtc cgacctccac
    11281 aaagcgttgt tagatcagca tgtcccggag gctgaccggg tggcgcaggc ccttgacctg
    11341 tatgtgtccg gctccctgaa cgtatttaac caccgtacca acgtggacat cggcaaccgg
    11401 cttgtgtcct ttgacattaa ggagctgggc aagcagctca aaaagctggg gatgctcatt
    11461 gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa ggccacatgg
    11521 tattttgccg acgagttcca tttgcttttg aaagaggaac agacggcggc ctattctgcg
    11581 gagatttgga agcggtttag gaaatggggc ggcattccaa caggtgccac ccagaacgtc
    11641 aaagacctgc tttcctcccc ggaaattgaa aatatccttg aaaacagcga cttcattacg
    11701 ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa tttgtccacc
    11761 gaacagcaga agtacatcga caattccgaa cccggcgagg gccttttgat ctttgaaaac
    11821 gtggtgctgc cctttaccaa ccccatcccg cacaacaccc agctttacaa gattatgacg
    11881 acccgcttaa acgaggtggc gggagtatga cccggcgacg ccacaaagga ggtgaaccac
    11941 catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg acgggctgac
    12001 gctggaaaat caggccaccg gcgaggtgga gaacatttcc agccgggagg ccgaacagga
    12061 tttgtccgcg ccggggccgg gcggcaccgc cgataagctg ctggaacggg ccgacgccat
    12121 ccatgacggc cataaggcca agaaagccgc gaagcaggcc ggggctgtgg ccgccgaggg
    12181 ggacgctgcc ctgcaccggc catccgcccg gctccaactg tcggaggaag aacgggccga
    12241 cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc tggataaggc
    12301 ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag
    12361 cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc cctccctgaa
    12421 acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg ggaaaatcca
    12481 tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg aacttgccga
    12541 gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga aaccctactg
    12601 ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt tctaccagaa
    12661 atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt cccggttctg
    12721 gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg gccagaccgc
    12781 aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag cggccaaggc
    12841 cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga aaggggcgct
    12901 ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac agtcctgcac
    12961 cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt ccgaggatag
    13021 cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc aagactatct
    13081 ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg acgagatcag
    13141 ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga tatttaccat
    13201 cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc tgacagaaaa
    13261 cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg cggagggcaa
    13321 cacccataca gacacctacc aagtgccgta tgactattac atctgctacg tcacgctgga
    13381 aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc taagtttcta
    13441 ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca gccagtaccc
    13501 caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg ctttggagga
    13561 tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg
    13621 gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa
    13681 tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca tctgcacccc
    13741 tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa cctacgacac
    13801 ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa
    13861 cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt acagttacgg
    13921 gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt gaccgcatcg
    13981 aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag cttcgggagc
    14041 tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg cgcggcatga
    14101 acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg
    14161 ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac cagacggatt
    14221 tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt tcccgttacc
    14281 gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac aaccgaaccc
    14341 gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga cgggacagaa
    14401 accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga cctgttttcc
    14461 gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca gatcaccacc
    14521 ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg
    14581 aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc caatgggacg
    14641 acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc ggagcgcatc
    14701 ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc taccggcgag
    14761 ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga actgttcaat
    14821 ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat tgacgacatc
    14881 ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg
    14941 ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag
    15001 gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac ccccgctgct
    15061 gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg cccggtatgt
    15121 aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc ggaggaaccc
    15181 gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg
    15241 gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc caaggctgaa
    15301 accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga tttggaggac
    15361 gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca ggaggacgag
    15421 gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg
    15481 tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat ctggtgagct
    15541 ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag cgtttcaaaa
    15601 aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc gtccccgacg
    15661 acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac gtgaccggcc
    15721 ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc gtctaccaga
    15781 tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag gacagtgcta
    15841 ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg
    15901 ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg cttttttccg
    15961 tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta aaaatgctgg
    16021 tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc gtccatatca
    16081 cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag gcaaacaccg
    16141 tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga
    16201 cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct aaccggcttt
    16261 tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta aagaagctgc
    16321 tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc gccgtggaaa
    16381 gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac ctttccccgc
    16441 agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt atccccaccg
    16501 ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag ctgctgtccc
    16561 tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg
    16621 ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc ctttcccccg
    16681 gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc gacgaggacg
    16741 ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca gacgtggccg
    16801 cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat acgcttttgt
    16861 ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc aagggattag
    16921 gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc tttgtccaga
    16981 gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg gtgctgccgg
    17041 aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc gagatcgcca
    17101 aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg cgggagctgg
    17161 tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc
    17221 ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa aactactact
    17281 gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca
    17341 agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa
    17401 agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt ttggctgata
    17461 ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca gtaacacagc
    17521 aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc
    17581 ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca gaacagagaa
    17641 aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca gtttttggaa
    17701 cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct gcaagccgcc
    17761 ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa cgggtattcc
    17821 tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc cgacgtggac
    17881 gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat cgcagagctg
    17941 aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat catcgactac
    18001 accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg acagttgggc
    18061 gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa agccgggatt
    18121 gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt tacccagtac
    18181 ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg
    18241 gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga accgcaggcc
    18301 cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat cccgatcatg
    18361 ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc
    18421 gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat gtccaagatg
    18481 cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc gattgtggcg
    18541 ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga gaaaggcatt
    18601 aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaac cgacccggcc
    18661 acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg
    18721 gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg caaggatttt
    18781 cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga tttctttgcc
    18841 gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga cggggccaag
    18901 ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag cgaattgcag
    18961 accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag
    19021 gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa
    19081 agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc
    19141 tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc
    19201 atccgggcca cggccagcga attgataacc gagatcgacg gccattttgc ccagcttcag
    19261 caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca
    19321 gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg
    19381 cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg
    19441 catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat
    19501 gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg
    19561 cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa
    19621 ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc
    19681 tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag
    19741 ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa
    19801 accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc
    19861 cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc
    19921 gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc
    19981 gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac
    20041 accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca
    20101 tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat
    20161 acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac
    20221 cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag
    20281 aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt
    20341 ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt
    20401 gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct
    20461 tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc
    20521 ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa
    20581 gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc
    20641 gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa
    20701 aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc
    20761 tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta
    20821 cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt
    20881 cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg
    20941 caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg
    21001 aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgatc
    21061 gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa
    21121 ccgttccagt cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc
    21181 attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc
    21241 actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat
    21301 gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca
    21361 tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag
    21421 cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag
    21481 ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg
    21541 cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt
    21601 ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc
    21661 attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg
    21721 ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa
    21781 gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga caagatactg
    21841 gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag
    21901 ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac
    21961 cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc
    22021 cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg
    22081 ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc
    22141 aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg
    22201 gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag
    22261 atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg
    22321 ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg
    22381 attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag
    22441 cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag
    22501 aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt
    22561 atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgccggtt
    22621 atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg
    22681 gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc
    22741 gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg
    22801 catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc tgcaaacatg
    22861 cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa
    22921 ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg
    22981 tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc
    23041 gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca
    23101 atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg
    23161 tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct
    23221 ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac
    23281 tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg
    23341 ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat
    23401 accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg
    23461 taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg
    23521 agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac
    23581 agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg
    23641 ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa
    23701 tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat
    23761 gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc
    23821 ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat
    23881 gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc
    23941 ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc
    24001 ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg
    24061 ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt
    24121 gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt
    24181 gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc
    24241 cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa
    24301 attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc
    24361 cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc
    24421 tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt
    24481 aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc
    24541 ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc
    24601 aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc
    24661 atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag
    24721 cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg
    24781 atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg
    24841 ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat
    24901 ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc
    24961 cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt
    25021 ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc
    25081 gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat
    25141 atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg
    25201 ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc
    25261 gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg
    25321 taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc
    25381 tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt
    25441 cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg
    25501 gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt
    25561 tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag
    25621 aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg
    25681 atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt
    25741 caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga
    25801 ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag
    25861 gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg gcccgtcgtt
    25921 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
    25981 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
    26041 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
    26101 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
    26161 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
    26221 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
    26281 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
    26341 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
    26401 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
    26461 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
    26521 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
    26581 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
    26641 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
    26701 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
    26761 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
    26821 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
    26881 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
    26941 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
    27001 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
    27061 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
    27121 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
    27181 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
    27241 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
    27301 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
    27361 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
    27421 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
    27481 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
    27541 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
    27601 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
    27661 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
    27721 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
    27781 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
    27841 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
    27901 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
    27961 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
    28021 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
    28081 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
    28141 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
    28201 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
    28261 tctatgccac accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg
    28321 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
    28381 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
    28441 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
    28501 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
    28561 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
    28621 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
    28681 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
    28741 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
    28801 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
    28861 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
    28921 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
    28981 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
    29041 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
    29101 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
    29161 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa
    29221 aaagtgggcg aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc
    29281 tccagtatgc gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca
    29341 catcaacatt gtaaatattt aaaacttaaa tgattttggg ctctttgtca aataggactg
    29401 atagagtgag ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat
    29461 tcataggtaa tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga
    29521 aagccataag cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca
    29581 tttgaataag gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt
    29641 tttatagaat gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa
    29701 gtgtccgcct ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt
    29761 cgtaattcgt cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc
    29821 caaccaaaat tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc
    29881 agaagccgag acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga
    29941 tcaaagcttt tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca
    30001 atgtgaaaac gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca
    30061 taagaaacat tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat
    30121 tgcataaaat aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt
    30181 ccagtatcgc catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga
    30241 aattcatcca ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc
    30301 agagaagcga gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc
    30361 gtcattgctt gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata
    30421 aagcagtttc tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag
    30481 tggtagcgtt gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga
    30541 atgagcgatt ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac
    30601 aaaggacagc gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc
    30661 ttaatgctct tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt
    30721 agtttgatat gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc
    30781 aattctaacg gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg
    30841 attttctatc agtcctagaa attatagagc cgattttgac cgacaggtat aaccctgccg
    30901 gtcttttttt gctgctacag gaaaactaca gactggctac agggaaagta cagatgcgct
    30961 tgccataata acaatcgtac cagccacaaa tcgtagtttt attgcaaagg aggcattcga
    31021 tcaaatggaa aaaagcaact atcattccaa tgcggatcat cacaaacggc atatgaaaca
    31081 atccgtggaa aagcgggctt ttctatgtgc gttcattatt tcgttcacag tctgcacgct
    31141 gtttttgggg tggagactgg cttccgtatt ggaggcaaca cagataccgc ccatccctgc
    31201 aactcataca ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg ctcttgccac
    31261 cgccaaagaa cagggagatg aacaggaatg gagcctgatt ttagtgaaca ggcagaaccc
    31321 catccccgca cagtacgatg tggagcttga gcaactatca aatggtgagc ggatagatat
    31381 tcggatttct ccctatcttc aagatttgtt tgatgccgca agaactgatg gagtttaccc
    31441 gattgtcgca tccggatacc gaacaacaga aaaacagcaa gaaattatgg atgaaaaaat
    31501 tgccgaatat aaggcgaaag gctacacctc tgcacaggct aaagcggaag cagaaacttg
    31561 ggtggccgtg ccgggaacga gcgagcatca gcttggtctt gctgtggata tcaatgcgga
    31621 cggaattcat tcaacaggca acgaggttta tagatggctg gatgaaaaca gctatcgttt
    31681 tggttttatt cgtcgctacc caccagacaa gacagagata accggtgtga gcaacgagcc
    31741 gtggcattac cgatatgttg gcatcgaagc tgccacagag atgtacaacc aaggggtttg
    31801 ccttgaggaa tatttaaaac cagaaaaatg agaaaaggat gttatgttat ggacagaaaa
    31861 agattgacac agcgtttccc gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc
    31921 ttttatgcgg gaatgagatt tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg
    31981 ctttcccatt tgctctttga aacggattgc gcattataca accacaatac cggatttgac
    32041 atgatatacc aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc
    32101 ttattgataa gaccggggga aaccttttct ttctggttgt tcctgcatac gccattgaca
    32161 attatccagc gcagaggtca cgaagtaaag gagtttccag agccaaacag tgacgagatt
    32221 aaaggggtgg atgcaaccat ttcagagggc tggattgatt taaaagtgcg aaacgatacc
    32281 gactgcacct accaaatatg ggtgacctta gatgatgaga aaatcatcgg tcaggtgttc
    32341 gccgacaaag agccccaagc attatacaaa attacaaatg gcagtatcca gtatgtccgt
    32401 gaaagtggcg ggatttatga atatgcccag gttaaacgga tgcaagttgc cttaggtacc
    32461 ggggaaataa tagattgcaa gctgctttat acaaacaaat gcaaaatctg ttatcccctc
    32521 ccggaaagtg tggatattca ggaggagaac caatgagaaa aagtgtgggc attactgttt
    32581 ttggatgcga acaggatgag gcaaatgctt tccgcgcttt atcgccggat tttcatatta
    32641 tccctacgct gattagcgat gcgatatcgg cagacaacgc aaaattggcc gctggcaatc
    32701 aatgcgttag cgtaagccat aagtccgagg tttccgaggc gacaattctt gcgctgagaa
    32761 aggtcggggt aaaatacatt tctacccgca gcatcggctg cgatcacatt gatacgactg
    32821 ccgccgagag aatgggaatc tcggttggca cggttgcgta ttcgccggac agcgttgcgg
    32881 attatgcttt gatgctgatg ctgatggcca tacggggtgc aaaacccacc atgcacgccg
    32941 tggcgcaaca agatttcaga ttggatcgta tccgggggaa agaactgggg gatatgactg
    33001 tgggagttat tggaaccggc catatcgggc aagcggtcgt caaaaggctg cggggatttg
    33061 gatgccatgt gctggcctat gataacagcc gaaaaatgga tgcagattat gtccagcttg
    33121 atgagcttct aaaaaacagc gatattgtta cgctccatgt gccgctttgt gcggataccc
    33181 gccatctgat cggtcagaag caaattggag agatgaagca aggcgcattt ttaatcaaca
    33241 ccgggcgcgg agcgcttgtc gataccgggg cgctggtgga ggcgttggaa agcggaaagc
    33301 tgggtggtgc ggcactggat gtgttggagg gcgaggatca gtttgtttat accgattgct
    33361 cgcagaaagt gcttgaccat ccctttttgt cgcagctttt aaggatgccg aatgtgatca
    33421 tcacacccca tacggcgtac tacactgagc gtgtgctgca ggatactaca gaaaaaacaa
    33481 tcaggaattg tctcaatttt gaaaggagtt tacagcatga atagaataaa agtcgcaatc
    33541 atcttcggcg gttgctcgga ggaacatgat gtgtcggtaa aagccgcaat agaaattgct
    33601 gcgaacattg atacggaaaa attcgatccg cactacatcg gaattacaaa aaacggtgta
    33661 tggaagctat gcaagaagcc atgtacggaa tgggaagccg acagtctccc cgccatactc
    33721 tccccggata ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg
    33781 cgtattgatg tggctttccc ggttttgcat ggcaaatgcg gggaggatgg tgcgatacag
    33841 gggctgtttg tattgtctgg tatcccctat gtgggctgtg atattcaaag ctccgcagct
    33901 tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa
    33961 tttcaaatga ttgataaagg tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt
    34021 gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa
    34081 cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg
    34141 atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt gattgtcggc
    34201 gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga aaacgagccg
    34261 gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt cgaggaacga
    34321 aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag agggcttgcc
    34381 cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt caataccctg
    34441 cccggtttta catcgtacag ccgctaccca cgtatgatgg ccgccgcagg aatcacgctt
    34501 cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt atggaaaatg
    34561 gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag tacgccacat
    34621 gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa
    34681 aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt ggctatggct
    34741 tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag
    34801 cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag cgagccgagt
    34861 tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc gcaattgatc
    34921 ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac ttcgatttta
    34981 tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca caaaatcgaa
    35041 gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc
    35101 actataagtt gattgatgag ccataccccg atacctattt taattttgcc gtttcataat
    35161 gaaagtattg gttttgtatc agattttgga tttgataaaa tctatgctgg tcatggacaa
    35221 tcttttactg gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta tagtattcaa
    35281 tctgcccagc ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc acgcccattt
    35341 tgtattttgc tggcggcggt tttgagaaat caaggccgcc gttcttttct ggcagcaaaa
    35401 ggtgtggcgg cccataggag gatgccgcta tgctgttttg ctattgccat gctctaatcc
    35461 acttccacca ctcaaaaaaa gccttgcccc tccatgaact gaaccccaaa agttgaacta
    35521 tttaatggac tgt
//
LOCUS       DAYYCM010000228        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.073, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000228 DAYYCM010000000
VERSION     DAYYCM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.073"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHAY1_003069"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHAY1_003069"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8594959.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHAY1_003070"
     CDS             complement(2159..3031)
                     /locus_tag="ACHAY1_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8594960.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHAY1_003071"
     CDS             complement(3373..4245)
                     /locus_tag="ACHAY1_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8594961.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHAY1_003072"
     CDS             4373..4942
                     /locus_tag="ACHAY1_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8594962.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHAY1_003073"
     CDS             5009..5746
                     /locus_tag="ACHAY1_003073"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8594963.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHAY1_003074"
     CDS             5977..7245
                     /locus_tag="ACHAY1_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8594964.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHAY1_003075"
     CDS             7732..8196
                     /locus_tag="ACHAY1_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8594965.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHAY1_003076"
     CDS             8180..8809
                     /locus_tag="ACHAY1_003076"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8594966.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHAY1_003077"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHAY1_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8594967.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHAY1_003078"
     CDS             10688..11134
                     /locus_tag="ACHAY1_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8594968.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACHAY1_003079"
     CDS             complement(11333..11848)
                     /locus_tag="ACHAY1_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8594969.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACHAY1_003080"
     CDS             11965..13167
                     /locus_tag="ACHAY1_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8594970.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAYYCM010000229        10087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.090, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000229 DAYYCM010000000
VERSION     DAYYCM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.090"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACHAY1_003081"
     CDS             353..523
                     /locus_tag="ACHAY1_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8594971.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACHAY1_003082"
     CDS             537..1139
                     /locus_tag="ACHAY1_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594972.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACHAY1_003083"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACHAY1_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACHAY1_003084"
     CDS             2143..2640
                     /locus_tag="ACHAY1_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8594973.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACHAY1_003085"
     CDS             2641..3057
                     /locus_tag="ACHAY1_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8594974.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACHAY1_003086"
     CDS             3077..4621
                     /locus_tag="ACHAY1_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8594975.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACHAY1_003087"
     CDS             4764..4988
                     /locus_tag="ACHAY1_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594976.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACHAY1_003088"
     CDS             5003..5872
                     /locus_tag="ACHAY1_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8594977.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACHAY1_003089"
     CDS             5853..6587
                     /locus_tag="ACHAY1_003089"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8594978.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACHAY1_003090"
     CDS             6620..7528
                     /locus_tag="ACHAY1_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8594979.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACHAY1_003091"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACHAY1_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8594980.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACHAY1_003092"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACHAY1_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8594981.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACHAY1_003093"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACHAY1_003093"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACHAY1_003094"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACHAY1_003094"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1571 c         2207 g         2761 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ctgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAYYCM010000230         9967 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.091, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000230 DAYYCM010000000
VERSION     DAYYCM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.091"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1107
                     /locus_tag="ACHAY1_003095"
     CDS             211..1107
                     /locus_tag="ACHAY1_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8594982.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1257..2198)
                     /locus_tag="ACHAY1_003096"
     CDS             complement(1257..2198)
                     /locus_tag="ACHAY1_003096"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8594983.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2324..3445
                     /locus_tag="ACHAY1_003097"
     CDS             2324..3445
                     /locus_tag="ACHAY1_003097"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8594984.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3703..5205
                     /gene="eat(A)"
                     /locus_tag="ACHAY1_003098"
     CDS             3703..5205
                     /gene="eat(A)"
                     /locus_tag="ACHAY1_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8594985.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5272..5799
                     /locus_tag="ACHAY1_003099"
     CDS             5272..5799
                     /locus_tag="ACHAY1_003099"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8594986.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6010..7071
                     /locus_tag="ACHAY1_003100"
     CDS             6010..7071
                     /locus_tag="ACHAY1_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8594987.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7074..7970
                     /gene="murQ"
                     /locus_tag="ACHAY1_003101"
     CDS             7074..7970
                     /gene="murQ"
                     /locus_tag="ACHAY1_003101"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8594988.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7984..9966
                     /locus_tag="ACHAY1_003102"
     CDS             7984..9966
                     /locus_tag="ACHAY1_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8594989.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         3281 a         1761 c         2176 g         2749 t
ORIGIN      
        1 atttaacaat gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt
       61 aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt
      121 tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga
      181 aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca
      241 aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa
      301 gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg
      361 atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc
      421 aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg
      481 aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca
      541 aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct
      601 agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca
      661 gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca
      721 catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg
      781 gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca
      841 tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat
      901 catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg
      961 gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca
     1021 gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat
     1081 gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa
     1141 acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt
     1201 gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat
     1261 tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt
     1321 gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca
     1381 gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg
     1441 tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct
     1501 tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct
     1561 ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt
     1621 ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt
     1681 aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc
     1741 aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga
     1801 ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt
     1861 acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg
     1921 gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag
     1981 tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc
     2041 acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca
     2101 ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg
     2161 ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa
     2221 tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa
     2281 atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc
     2341 aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg
     2401 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
     2461 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
     2521 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
     2581 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
     2641 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
     2701 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
     2761 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
     2821 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
     2881 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
     2941 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
     3001 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
     3061 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
     3121 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
     3181 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
     3241 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
     3301 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
     3361 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     3421 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     3481 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     3541 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     3601 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     3661 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     3721 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     3781 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     3841 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     3901 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     3961 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     4021 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     4081 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     4141 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     4201 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     4261 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     4321 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     4381 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     4441 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     4501 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     4561 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     4621 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     4681 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     4741 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     4801 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     4861 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     4921 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     4981 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     5041 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     5101 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     5161 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     5221 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     5281 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     5341 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     5401 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     5461 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     5521 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     5581 gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt
     5641 cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat
     5701 gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca
     5761 ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa
     5821 ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc
     5881 ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg
     5941 aaattttgtt tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag
     6001 agggataata tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa
     6061 cagtatatcc gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata
     6121 ccagaagatg atgtttcggc atatcaaaag cgattgatcg aactaggaag cgaagcgaaa
     6181 aagaataaac tagaattgat ggtcgatatt tcaggagagg cattggaacg agcgggtttt
     6241 tcattagaag atctaacgcc tttgaaagca attggtgtaa caggtttaag aatggattat
     6301 catatttcca atcagcagat tgccgactgg tcacatcaat tgaaaatcag tttgaatgct
     6361 agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga tttctcacag
     6421 attgaagctt ggcataatta ttatccacgt cccgaaacag ggctggacaa agaatggtat
     6481 cagaaaaaaa atcaatggct caaaattcaa ggtctccttg tccaaggttt tgtaccagga
     6541 gatacagagt tgagaggccc actatatcaa ggattgccaa ctttggaaga acatcgaggg
     6601 atccatcctc ttgcaggcgc tctagatcta ttagcaagta acacggatat tgtttacatc
     6661 ggagatgcag gtctgtcaga aaacgttcag gagcaatttg cttctttcca aaaagaacag
     6721 acagtcttgc tccatacgga acctgtagat gaagaatttt acgaatatat attagggaag
     6781 catacgaatc ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc acgttttcga
     6841 gagattccgc ctatcccagc aagaaataca gctacgcgta tgaaagggtc tattacttta
     6901 gataacgaaa agtatttgcg ctatatgggt gagatccaat taaccaaata tgatttgcca
     6961 gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag aagagctacc attgatcaac
     7021 caaatcaaag ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca
     7081 aaatcaattt agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg
     7141 agatgagtat ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa gtggccgctg
     7201 cggttgaaaa agaactgaca agtatcgaac cagtgattgc taacacgatt gagtccttta
     7261 aaaaaggtgg acgtctgatt tatatgggag ccggaacaag tggaagacta ggtgtgttag
     7321 atgctgctga atgcgtaccg acattcggtg tagaaccgga aatggtcata ggtctaattg
     7381 ctggtggaga agaagcaatg actgtagctg tcgaaggggc cgaagattct tatgagctag
     7441 ggcaagaaga tctaatcaag ctgaatttga caaaaaatga tatagttatt ggtatcgcag
     7501 ctagcggtcg tacaccatat gtgatcggtg gattagatta cgcaaaatcg atcggtgctg
     7561 tcacaggaac gatttcttgc aataaacaag ccgaaatcag taaacacgcg gatttcccaa
     7621 tcgaagtcga tgcaggtcca gaatttttga caggctctac tcgattgaag tcaggaacag
     7681 cacaaaaact tatcttgaat atgatttcta ctatcagtat gattggtata ggtaaagtat
     7741 tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac
     7801 gaatcatcat gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac tttgaagaag
     7861 cagatcaaaa tgttaagctg gctatcgtca tgattttgac aaatagtgat aaagaggaag
     7921 ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg gaccttatga gggggaaaag
     7981 accatggcaa aaaacgaact gactttggaa gaactggcaa aacaaattta taaaggggca
     8041 ggcggcatgg gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc
     8101 atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta
     8161 gtagaagatg aacagcttca agtcatcatc ggacctggga aagtgaacaa agtagcacag
     8221 caaatggttg atttggcagg cgtgaaatta ggagaaaaac tttcttctgt aagtacagct
     8281 acagcaaatt catcagcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa
     8341 gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa agacatttct
     8401 aatatctttg tgccaatgat tcctgcattt gtcggtactg ggatcgtggc agggattgcc
     8461 gcagttttag caaacttagt cacagccgga acacttgatg cagcaacatg gcaacaatat
     8521 atcgatgtga tgaacatctt gaaaaacggg atgtttagct acctagttat ctatacaggt
     8581 atcaatgctg cacaagtttt tggtgcaaca ccaactcttg gtggagttat cggggccgtt
     8641 gtgatgctga cagggatgaa tccggaagca ccactgaaaa acttatttac tggtgaagct
     8701 cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct attgtcaatc
     8761 gttgagaaaa aactgcataa agtagtacct gatgcggtag atatcatcgt tacaccaact
     8821 ctttcattga ttgccatcgg tctgattgaa attttcttca tcatgccttt agcaggtttc
     8881 atttctgatg gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg agcatttgcc
     8941 ggcttcgttc taggcacatt attcttgccg atggttatgt tcggactgca tcaaattttg
     9001 actccaatcc atgtgcaaat gattgatgaa actggtcgta cgctattgct tccaattctt
     9061 gcgatggctg gtgcaggaca agtgggtgca gcattggcat tatggattcg ctgcaaaaaa
     9121 gataaagaat taacagaaat gatcaaagga gctttaccag tcgggatcct aggaatcggc
     9181 gaaccattga tttacggagt aacattacct ctaggacgcc catttattac tgcttgtatc
     9241 ggtggaggta tcggcggggc agtgatcgga atgatcggaa atgtcggggc tatcgctatt
     9301 ggaccttcag gagccgcttt gatcccattg atctctgacg gtaaatggtt aggctacatc
     9361 cttggattat tggcagctta tgctggtggc ttcgttgcta cattcttctt cggtattcct
     9421 aaagaccgtt tagaaaaaga agaattagcg actgaaacag ccacagtaaa tgaagtgcct
     9481 gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta tcctctcatc agttgctgac
     9541 ggaacagttg tttctttaga taaagcaagt gatccagttt ttgcacaaaa gatgatgggt
     9601 gaaggctact ttgtagaacc agaaaacgga catatctatt ctccagttac tggggtagtc
     9661 agctctgtct tcccaacgaa acacgctatt gggatcacaa cgccaaatgg tttagaaatc
     9721 ttgcttcata tgggaatcaa cacagttgac ttaggcggta cgccatttga tgtgaaagta
     9781 acagaagggc agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcgatc
     9841 aaagcagcag gcaaagaaac atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa
     9901 aactttgtct tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa
     9961 gcttaat
//
LOCUS       DAYYCM010000231         7000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.110, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000231 DAYYCM010000000
VERSION     DAYYCM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.110"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1267
                     /gene="mef(H)"
                     /locus_tag="ACHAY1_003103"
                     /gene_synonym="mefH"
     CDS             29..1267
                     /gene="mef(H)"
                     /locus_tag="ACHAY1_003103"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8594990.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1494..1913
                     /locus_tag="ACHAY1_003104"
     CDS             1494..1913
                     /locus_tag="ACHAY1_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8594991.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1918..2157
                     /locus_tag="ACHAY1_003105"
     CDS             1918..2157
                     /locus_tag="ACHAY1_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8594992.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2382..3305)
                     /locus_tag="ACHAY1_003106"
     CDS             complement(2382..3305)
                     /locus_tag="ACHAY1_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8594993.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3337..4101)
                     /gene="map"
                     /locus_tag="ACHAY1_003107"
     CDS             complement(3337..4101)
                     /gene="map"
                     /locus_tag="ACHAY1_003107"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8594994.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4259..4699
                     /locus_tag="ACHAY1_003108"
     CDS             4259..4699
                     /locus_tag="ACHAY1_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8594995.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(4877..5446)
                     /locus_tag="ACHAY1_003109"
     CDS             complement(4877..5446)
                     /locus_tag="ACHAY1_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8594996.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            5701..6933
                     /locus_tag="ACHAY1_003110"
     CDS             5701..6933
                     /locus_tag="ACHAY1_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8594997.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         2208 a         1236 c         1304 g         2252 t
ORIGIN      
        1 gtttgattaa ataaaaagga gagattttat gcaagcagaa aaaaaacttt ttaatcgtga
       61 ttttatactt gttgttatag gacagataat ttctttattt ggaaacgcta tcctacgatt
      121 tgcattaccg tactatttgt taaaagaaac tggttcatca gctttgtttg gtattgctac
      181 agcctgctct tttttaccta tggttatttt gtcgctggta ggtggtatta ttgctgacag
      241 gacaaataaa agaaacatta tggtcatact agattttgta acagcaatca taattttagt
      301 attttatttg tcattgggaa aaattcctat catacctttg tttattgttg ctttgatgtt
      361 actttatggt atttctggtg catatcaacc agcggtacaa gcaagtattc ctctattggt
      421 atcatcagat aaacttaccg tcggtaatgc tataataaat caagtaaata ctttagcaaa
      481 ctttatcgga ccagctataa gcggagtagt ttttcaattt tatggtatca atcccatttt
      541 aattgttagt attatatgtt ttactttttc agccattatg gaaattttta tccatattcc
      601 acaccaaaaa agacctcgtg aagtaggtgt aattgcagtt gctaaaaacg atttaaaaga
      661 tagttatacc tttttgaaaa aggagaaacc catttttata tccgtaatct ttttggtttg
      721 cattttcaat ttggttttaa gtgctgtttt aattgttggt ctgcctatct taatttctga
      781 agttttaaaa atgccagata cactatatgg tttttcacaa tcagcattat cattaggtgg
      841 tttatgtggt ggtattttaa cagctcttgt tgcttcaaag ttaaaactaa aaaaccttta
      901 taccttgtta cttatttgtt ctgtttctgt tttacttatg ggaatttcgc tatttttaga
      961 attgcctgca atagtttcct actgggtagt tacagtgatg tgctttgtgg ctatgggtgt
     1021 atctactatg tttgttgttc aaatttacac tcttgttcag acacaaacac caccgcaatt
     1081 agtaggaaag ataatggcta cgctggtagc agttgctatg tgcggtcaac ctattggaca
     1141 aatggtttac ggtattttat tcgatgtatt tatacaagaa acatgggttg ttatgtttgt
     1201 agcttctatt gcttcaatca tgatttcaat atattcaaaa agaatttttc aaaagttaga
     1261 gatataatac aaggggttaa atagtatgtt aggctaaaaa caatatataa aagaaaattt
     1321 gaaatctgct aaaaggatag tgttataaaa gaaagagtaa tattgcagta tttaaggctc
     1381 gcacaaatat atgttttgtg tgggcttttt tcttaccatt tttctgtatt tctaaagggt
     1441 ataaacggta ttccgtttat atatttcagc attagcagaa agggggtgag attatgagga
     1501 catcttcttt cgagcatatc gtcagattac agttcaattc tttaatgctg attgtcatta
     1561 agaacaaagt gaaaagtcga aacagacagc ttgcaagaca ttctaaacgt gaagtattgt
     1621 tttgtgaaat agccgaaacg aaacaagttg aacgtggaac gattgatacc tgtttttatg
     1681 attgtgtatc atttaaagtc ttgcacttta caatttatgt atctgatgaa acacttggta
     1741 cagttttaca tagactgtca gaaaaacagc gtagtgcgat tttattacgt tattttcaag
     1801 gaatgaacga cagagaaatt tcagaactgt accatgtatc acgttctgct atttgcagta
     1861 gaagaactag aggactaagg aagttaaaaa tgcttttgag cgaaaggaag taaagaaatg
     1921 agtaaagatt atggctatcc ctcttttgaa ttgatttgca aagcgtcaag cggagatgaa
     1981 atggcagtta aagagatttt gaagttttat gacggttata tttctaaatt atgcttgcgt
     2041 cctttttacc actctgaaag tggtaaaatc attatgcagg ttgatgaaga actaaaagga
     2101 gaaattcata cagacatgat gaaagcaata ttgaaatttg aaatcggagt gaagtaatca
     2161 actgtataaa aaaatcggga gatacacagt ttcgtgctat ctcccttagt agttttacgg
     2221 tcatatcaag gctcattcaa tcgtggtaaa ttcgtggtaa gatgagtgtt tttctatact
     2281 tcaaaatgct tgaatgtata gtgtttatgc ggataattga aaattagata taaaatttca
     2341 aataaattac tgatataaat ttacaataag aactcttgtt tctacttttt tttatgagaa
     2401 aatttagttt tcaatttatt gaaccatttt cggcttggat ctcgacgttt tttgatttga
     2461 tgacccgtca cgtattcttg aacaactgca ttgatgattc cgcccaaaat gatgatcgta
     2521 gcagcaaaat taagccaaag catgagtaca ataaaacttc ctatgatctg ataaccgctg
     2581 atcctcgttg caaaatacct agcatagata ctaaacactt gtgataacag catccagccg
     2641 acagtagcga agatcgtgcc cggaatgatc gtcttcaact tcaactgagc attagggaca
     2701 attaaataaa tcaagaacat gatgacaaat aaagctacca atgtgattgg ccacttcaaa
     2761 gtttggaact gatcgataaa gttcacgggt atttgtacga ttggctgaat agcatccaaa
     2821 atcagctgtc ccaacccaag aacgatagtt accccactaa tagcaatcat gaacaagaca
     2881 atgacaatta gtgagaagaa acggacgatc aaaaaatttt ttctattttc tacgccatac
     2941 gctttattca tcgcaatttg caaagcattg atactttgac ttgcagacca taaagccgct
     3001 aatgcagaaa cggataacaa gcctcctgaa ctttgtgtca aaaggtcttt gattgcagga
     3061 ccgataaact ggtaaacggt ctcaggaatc acttctcgta aataggtcag tacagtctct
     3121 tgatcgatgt ggagaaacgg taaaatatta ccaaaagcaa taatcaatgg aaataaagac
     3181 aagagtaaat aataagcaac gacgacagag gtcgtactca tttctgaatc agtgatgcgc
     3241 ttttgagtcg tctcaataaa gcgcatcaga ttttcatttt gttttatttt gttccaaact
     3301 ttcatgtacc tctcccgtac ctattgactg gatagatcaa taagtgcctt cttctccttg
     3361 tgatgtcaaa attcttggac catctttagt aatagccaat gtatgttcat attggcaaga
     3421 aagtccgccg tctcttgtat acgcagtcca gccgttagga tccattttca ttttccaagt
     3481 tccggtatta accataggtt cgatcgtgat aaccatgcct tctttcaacc gcaacccttt
     3541 tccagcttct ccgtaatgag gaactgctgg ttcttcatga atcgtaggac caacgccatg
     3601 accaataaaa tcacgaacga tcgctagatt ttcgccttct acataagttt ggatagcatg
     3661 accgatgtca ccaatccgat tccctacttg tgcttgctca ataccgatgt acaaagcttt
     3721 cttcgttact tccatcaatc ggtcgatctc aggtgtagat tctccaacta cataggccca
     3781 acaagaatca gaaatgccgc ctttcaaatc gatacacata tcgactttta ccaaatcacc
     3841 gtttttcaaa ggctttttac gagggaatcc atgacaaatt tcatcattta tactgataca
     3901 agtcgcatat tcataccctt caaagccaat ttgcgcagca attccgccat gactctcgat
     3961 atagtttctg acaaatacct caatatccca acttgtgatt cctggtttta taaaatcacg
     4021 cagattttta tgtacatctg ctaataaagc tccagattct gccatcattt caatttcacg
     4081 tgctgatttt aaagtaatca tattttcgtc tcctttttac aaatccctct cattttaaca
     4141 tagcggcata catttagcca aatgacattc cccaggtgtc tctaggtttt attttctttt
     4201 caatgatgtt ttggtataat tcataattgt taaataatta taaacgagga ggcacatcat
     4261 ggctttagca aaaatcgtat atgctagcat gacaggaaac actgaagaaa tcgccgatat
     4321 tgttgcagaa gcatttgaaa atctggatat cgaagtcgaa atcaacgaat gtacccaagt
     4381 agatgcagat gaattcgagg aggcagatat ttgcgtagta gctacttata catacgatga
     4441 tggcgatctg cctgatgaga tcgtagactt ttatgaagac ttgcaagaat tggatctatt
     4501 gggaaaaatt tacggtgtct gcggatctgg tgatactttt tacgatgaat tctgcaaatc
     4561 ggtggatgat ttcgatgctg cttttgcaaa aacaggcgca tcaaaaggag ccgaaaatgt
     4621 taaagtggat ctgaatgctg aagaagaaga tatcgaaaat ttagaagctt tcgctaaaga
     4681 actagtcgca aaaatttaat atacttgcaa taaaagagcc atccagattt attcaatctg
     4741 tatggctctt ttattcattg cattcatgga agagttcttc gggagttaaa aatgcatacg
     4801 ctgtaagaaa aacgaacttt tccttatttt gacacatctc ttgttaccta ctgatttgtg
     4861 gaacttttga gtatattcaa tcgatttcga ttggttcttt gaatttcaag atagcatcta
     4921 tactgtcttg gatcatcttt tcaatctgtt catcagttgg atggatgatg cttgaagaca
     4981 tcaaagctgc gatcccagta gctatgatcc atgtcccatt atacaaagat tcgatctgct
     5041 cttctgaaag attcacataa gctggatcgc gtttcactgt attcacaaag taagtataag
     5101 agaaattctg catctctttt cctccaccat attcttctaa gtacagtgaa cgatacaact
     5161 tcttctctct tcttgcaaaa tggatataat taaaagcaag atcgataatc gtattccctg
     5221 tatgctcaac agtgaacact tcatattgta aatgatcata gatctgctca aaaagagctt
     5281 gtctcaagtc atccatattt ttaaactcaa gataaatcgg ttgcgtggaa caattcattt
     5341 tgttcgcgat gtttctcgca gtaaatcgtg taaatccatc ggtggcagct acttcataag
     5401 cagctgccaa aatctgttct ctagtaatcg tcttttttct tgccatattt tttaatctcc
     5461 ttaatttttt tataacgctt ccaccattga ttaatttcat tgtaacatgt tttttttgat
     5521 atgcaaaatt agtttttgat tttcttcaca agctgaaata gcaagtagta acatatatca
     5581 tgaaacttaa ccttaaaaaa ataacctcct ttttttattt aaagcctcat tgtttgaatc
     5641 ataattaaat ggtatcatgc taatagttac cagaaaaaat ggtgaggagg aatttttatt
     5701 atgttatctg attttaatga acttctaaaa aaatatgcac gcttaattgc agaaactggc
     5761 gtagccacag aaaaaggaca cacagttgtt ttacagatca gcgtggaaca ggcacccctt
     5821 gctcgattga tcacacaaga agcttataaa ctaggagccg ctgaggttat cgttcaatgg
     5881 actgacgacc agatccaaag agaatttctt ttacatgctg caacggatcg tattgagaat
     5941 gtaccacaat ctaaaatcga tcaagcggat gaatggcttg aaaaaggagc aagcaggatc
     6001 agcgttgttt ctgccgatcc cgatgcattt gctggtgtag atagccatcg agtagcgtct
     6061 tatcaatcag cgaatggcaa agcactaatg aatttacgca aggcaactca agcaaataaa
     6121 gtcagttgga ctgtcgtggc tgcggctggt aaacaatggg cagcaaaagt cttcccagaa
     6181 ctgccagaag aaaaacaagt tgaggcatta tgggatcaga ttttcaaaac gactcgtgtc
     6241 tatgaagaag accctgtctt agcatggaag aagcatgatg aaaaattagc aaaaaaagca
     6301 gaagaattga atcgagaaca attttctgcc cttcactata ctgctcccgg tacagatatc
     6361 attatcggat tgcctaaaaa ccatctttgg gaaggcgctg gaagctataa tgctcgtgga
     6421 gaaaaattca tggcaaatat gccgacggaa gaagtgttca cagcacctga cagtcatcga
     6481 gtagatggtt acatttcaag tacaaagcca ctgagttatg ctggcaccat catctcgggt
     6541 atgaaattta cttttaaaga tggaaaagtt gttgattttt ctgccgaaca aggagaagat
     6601 gtcttagcaa aactgcttga tacagatgaa ggtgcgcgtc gtttaggaga agtcgcactt
     6661 gtacctgacc cttcacctat ttctcaatct ggcatcatct ttttcaatac attatttgat
     6721 gaaaacgcat cgaatcactt agcactaggt tctgcttatg ccttcagtgt gaaaggcggt
     6781 acagaaatgt ccgatgaaga attagcagaa gctggattga atagaagtca aacccacgta
     6841 gatttcatgg taggatctga caagatggat atcgacggaa tccgtgaaga tggctctaca
     6901 gtaccgattt tccgaaatgg cgattgggct tgagtatact ttcgcacaca tgcaaaaaat
     6961 aatggctctt tgtcaataag gactgatgag ttgtgcaaaa
//
LOCUS       DAYYCM010000232         4272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000232 DAYYCM010000000
VERSION     DAYYCM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.134"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..621
                     /gene="dfrG"
                     /locus_tag="ACHAY1_003111"
     CDS             124..621
                     /gene="dfrG"
                     /locus_tag="ACHAY1_003111"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8594998.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(693..2645)
                     /locus_tag="ACHAY1_003112"
     CDS             complement(693..2645)
                     /locus_tag="ACHAY1_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8594999.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2652..2888)
                     /locus_tag="ACHAY1_003113"
     CDS             complement(2652..2888)
                     /locus_tag="ACHAY1_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8595000.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3290..3580
                     /locus_tag="ACHAY1_003114"
     CDS             3290..3580
                     /locus_tag="ACHAY1_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8595001.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3852..4201)
                     /locus_tag="ACHAY1_003115"
                     /pseudo
     CDS             complement(3852..4201)
                     /locus_tag="ACHAY1_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1344 a          722 c          746 g         1460 t
ORIGIN      
        1 attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca ggataatgag
       61 aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta
      121 taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat
      181 gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat
      241 ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga
      301 aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca
      361 atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa
      421 cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat
      481 gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt
      541 gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa
      601 agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca
      661 caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa
      721 tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct
      781 gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca
      841 tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat
      901 ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg
      961 tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt
     1021 ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta
     1081 tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga
     1141 cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat
     1201 cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat
     1261 tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt
     1321 aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc
     1381 attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta
     1441 taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat
     1501 tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa
     1561 gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt
     1621 cttgtatctt catttaattc atccattgaa atattccaat cataggcaac atcagaacgg
     1681 aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct
     1741 tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag
     1801 tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc
     1861 tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag
     1921 gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc
     1981 atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct
     2041 ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg
     2101 cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct
     2161 tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt
     2221 tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag
     2281 ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta
     2341 tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg
     2401 tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga
     2461 tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt
     2521 gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt
     2581 gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg
     2641 gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta
     2701 cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat
     2761 tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc
     2821 cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga
     2881 aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca
     2941 atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat
     3001 tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca
     3061 gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact
     3121 acttaaatct atactgctgc ctcttttttt tagtacatgt ccttcatctt cgcaggaagt
     3181 gccaatatga ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct
     3241 tctttacaaa gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa
     3301 atatggaaca gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt
     3361 cagtttaaat ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt
     3421 agacgaaggg atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt
     3481 gagatcctat catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata
     3541 cccaagaaaa aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa
     3601 aaatgtccta gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac
     3661 gagcatacgc tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga
     3721 tgaatcacta aactttccaa actcatttga gggatattgc ttaaagattg aacagatcaa
     3781 ttcgaagccc tattagtatt cgctcaatgg ggggatcaga ggccataaaa agagactagt
     3841 cattactagt ctcaggtagt ggaattgtag gattttaaac gttcctggag agtaccggta
     3901 tgtaactcga agaggtgatt atcatagtcg taaaagtaaa ttgaagaagc ctcgcctttc
     3961 acacgagaac gtcctggttc gacagttaaa cctaacattt ggatttttga gacaaacgaa
     4021 tctatatctt gttcgtcaat tttaaaagca atatgattgt atgtctttgg cagtttattc
     4081 gacgaattct gcattactgc aatccatagg tccttaacta agaaaaattt ttctggatat
     4141 aatgaaaatt tttttgttga ctatcgtaga tttcttttgc atcaaataag gtatttaaca
     4201 attaagtatt taaaagataa cttttaagtt atctttaagt aatgcttaaa gctatgttat
     4261 actctattag ga
//
LOCUS       DAYYCM010000233         2771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000233 DAYYCM010000000
VERSION     DAYYCM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.156"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHAY1_003116"
     CDS             110..193
                     /locus_tag="ACHAY1_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8595002.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHAY1_003117"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHAY1_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8595003.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHAY1_003118"
     CDS             complement(1318..2181)
                     /locus_tag="ACHAY1_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8595004.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHAY1_003119"
     CDS             complement(2183..2455)
                     /locus_tag="ACHAY1_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8595005.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHAY1_003120"
     CDS             complement(2472..2681)
                     /locus_tag="ACHAY1_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8595006.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DAYYCM010000234          958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5841
            SAMEA115707986-rid22593743.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAYYCM010000234 DAYYCM010000000
VERSION     DAYYCM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707986
            Sequence Read Archive: ERR13197711
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290193.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:46:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,121
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 37 of 93
            Pseudo Genes (incomplete)         :: 44 of 93
            Pseudo Genes (internal stop)      :: 28 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707986-rid22593743.guided.205"
                     /isolate="VRE5841"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..893
                     /gene="aac(6')"
                     /locus_tag="ACHAY1_003121"
     CDS             345..893
                     /gene="aac(6')"
                     /locus_tag="ACHAY1_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8595007.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          340 a          138 c          191 g          289 t
ORIGIN      
        1 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
       61 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
      121 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
      181 ctttcctatt ttgaataaag tgtttctgta taatttttta ttttataagt aagataaata
      241 atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt
      301 ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat
      361 ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc
      421 cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa
      481 tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat
      541 acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc
      601 aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca
      661 cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt
      721 atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat
      781 tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca
      841 aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag
      901 aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa aattactt
//
